Rw2G003600

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008099
Physical Location & Seq
Forward (+)
3321421 .. 3325112
3692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003600.1

Sequence Viewer

Length: 1176 bp
ATGGCTAGCACACAACTTTCCGACCAAGTTTTCAGGCCAATCCCTGCCGTGTGCGTCAGTTTGCGGCGACTGAGCACCACTAAACCGAATAAACTACCATGCCACCCACGTCCCAAAGCCTTGAAACACAAACACTTGGGGATTCAAACAGCGCAGAACTACTCAAAGTGGGCTGTTAGACTTAGCTTAGTAGACCAAAGCCCGCCAAAATCAACCGTCGACATGGAACGGTTAGTCGATTTTCTGTACCAGGATTTAGTGCACTTGTTTGATGATCAAGGAATTGATCGGACGGCATATGATGAACGTGTGAAGTTCAGGGATCCAATCACCAAGCATGACTCTATAACTGGCTACTTGTTGAACATTGCCTTCTTGAAGACAGTCTTCACTCCCAAGTTCCAGTTGCATTGGGTCAAACAGTCAGGACCATATGAAATCACTACAAGGTGGACTATGGTTATGAAATTCATCCCTCTGCCTTGGAAACCAGAATTGATCTTCACTGGTACTTCTGTTATGGGAATCAACCCTGAAACCGGGAAGTTCTGCAGCCATGTGGACTTCTGGGATTCCATAAGGAAGAATGACTATTTTTCTGTAGAAGGTTTGTTAGATGTATTCAAGCAGCTGAGGATTTACAAAACTCCAGACTTGGAAACACCAAAATATGAGATACTAAAGAGAACAGCAAATTATGAGGTAAGAAAGTACTCCCCTTTTATAGTGGTAGAAGGAAGTGTAGACAAGCTGTCTGGATCAGCTGGTTTCAATGATGTTACTGGGTACATCTTCGGAAAGAACTCGAAAATGGAGAAGATACCAATGACAACTCCAGTGTTCACAGAGGCATCTGATGCTGAATTTTCCAAAGTATCCATCAAAGTATGTCTTCCACTAGACAAGGATTTAAGCAGTTTACCAGATCCTAATGCAGAAACAATTACCTTGAAAAAAATGGAAGAAGGCATTGCTGCTGTTACCAAGTTCAGTGGGAAGCCCACCGAAGATGTTGTTCGTGAACAGGAGAAATTGCTGCGTGCTAGTCTTATTAGAGATGGTCTCAAACCTAAGGCCGGTTGTATGCTTGCTCGTTACAATGACCCCGGCCGAACTTGGAGCTTTATACTGAGAAATGAAGTGCTGATATGGCTGGAGGAATTCACATTGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358 SOUL

Protein Analysis

391

Amino Acids

44.76

Weight (kDa)

8.93

Isoelectric Point (pI)

28.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 77 - 186 2.8e-28 Uncharacterized conserved protein (DUF2358)
SOUL PF04832 219 - 383 1.4e-37 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 192, 219, 744
AciI CCGC 2 cut(s) 64, 203
AclWI GGATC 4 cut(s) 317, 330, 766, 920
AcoI YGGCCR 1 cut(s) 1108
AcsI RAATTY 3 cut(s) 467, 863, 1160
AfaI GTAC 4 cut(s) 248, 511, 713, 788
AfiI CCNNNNNNNGG 2 cut(s) 539, 1076
AflIII ACRYGT 1 cut(s) 307
AgsI TTSAA 7 cut(s) 124, 146, 364, 379, 625, 772, 952
AhdI GACNNNNNGTC 1 cut(s) 751
AjiI CACGTC 1 cut(s) 110
AjnI CCWGG 1 cut(s) 249
AluBI AGCT 5 cut(s) 186, 631, 751, 764, 1122
AluI AGCT 5 cut(s) 186, 631, 751, 764, 1122
Alw21I GWGCWC 2 cut(s) 77, 264
Alw26I GTCTC 1 cut(s) 1067
Alw44I GTGCAC 1 cut(s) 260
AlwI GGATC 4 cut(s) 317, 330, 766, 920
AoxI GGCC 3 cut(s) 35, 1074, 1108
ApaLI GTGCAC 1 cut(s) 260
ApeKI GCWGC 4 cut(s) 552, 628, 974, 1036
ApoI RAATTY 3 cut(s) 467, 863, 1160
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 1 cut(s) 428
AsuC2I CCSGG 2 cut(s) 541, 1107
AsuHPI GGTGA 1 cut(s) 322
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 1 cut(s) 428
AxyI CCTNAGG 1 cut(s) 1071
BaeGI GKGCMC 1 cut(s) 264
BaeI ACNNNNGTAYC 2 cut(s) 501, 534
BamHI GGATCC 1 cut(s) 322
BbsI GAAGAC 3 cut(s) 379, 386, 884
Bbv12I GWGCWC 2 cut(s) 77, 264
BbvCI CCTCAGC 1 cut(s) 632
BbvI GCAGC 4 cut(s) 564, 640, 961, 1023
BccI CCATC 2 cut(s) 887, 1052
BceAI ACGGC 2 cut(s) 32, 309
BciT130I CCWGG 1 cut(s) 251
BciVI GTATCC 1 cut(s) 886
BclI TGATCA 1 cut(s) 274
BcnI CCSGG 2 cut(s) 541, 1107
BcoDI GTCTC 1 cut(s) 1067
BfaI CTAG 3 cut(s) 6, 899, 1044
BfmI CTRYAG 2 cut(s) 550, 600
BfuI GTATCC 1 cut(s) 886
BisI GCNGC 5 cut(s) 65, 553, 629, 975, 1037
BlsI GCNGC 5 cut(s) 66, 554, 630, 976, 1038
BmcAI AGTACT 1 cut(s) 713
Bme1390I CCNGG 3 cut(s) 251, 541, 1107
Bme18I GGWCC 1 cut(s) 428
BmeRI GACNNNNNGTC 1 cut(s) 751
BmgBI CACGTC 1 cut(s) 110
BmgT120I GGNCC 1 cut(s) 428
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 3 cut(s) 251, 541, 1107
BmrI ACTGGG 1 cut(s) 792
BmsI GCATC 2 cut(s) 847, 860
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 792
BpiI GAAGAC 3 cut(s) 379, 386, 884
BplI GAGNNNNNCTC 2 cut(s) 1047, 1079
BpmI CTGGAG 3 cut(s) 633, 819, 1175
Bpu10I CCTNAGC 1 cut(s) 632
BpuMI CCSGG 2 cut(s) 541, 1107
BsaI GGTCTC 1 cut(s) 1067
BsaJI CCNNGG 2 cut(s) 482, 1105
Bsc4I CCNNNNNNNGG 2 cut(s) 539, 1076
Bse118I RCCGGY 1 cut(s) 1076
Bse1I ACTGG 5 cut(s) 355, 403, 511, 787, 836
Bse21I CCTNAGG 1 cut(s) 1071
Bse3DI GCAATG 2 cut(s) 366, 969
BseBI CCWGG 1 cut(s) 251
BseDI CCNNGG 2 cut(s) 482, 1105
BseGI GGATG 1 cut(s) 471
BseLI CCNNNNNNNGG 2 cut(s) 539, 1076
BseMI GCAATG 2 cut(s) 366, 969
BseMII CTCAG 3 cut(s) 62, 623, 1121
BseNI ACTGG 5 cut(s) 355, 403, 511, 787, 836
BseSI GKGCMC 1 cut(s) 264
BseX3I CGGCCG 1 cut(s) 1108
BseXI GCAGC 4 cut(s) 564, 640, 961, 1023
Bsh1285I CGRYCG 1 cut(s) 1111
BshFI GGCC 3 cut(s) 37, 1076, 1110
BsiEI CGRYCG 1 cut(s) 1111
BsiHKAI GWGCWC 2 cut(s) 77, 264
BsiSI CCGG 3 cut(s) 540, 1077, 1107
BslFI GGGAC 1 cut(s) 96
BslI CCNNNNNNNGG 2 cut(s) 539, 1076
BsmAI GTCTC 1 cut(s) 1067
BsmFI GGGAC 1 cut(s) 96
BsnI GGCC 3 cut(s) 37, 1076, 1110
Bso31I GGTCTC 1 cut(s) 1067
Bsp1286I GDGCHC 2 cut(s) 77, 264
Bsp143I GATC 6 cut(s) 274, 286, 322, 498, 758, 925
BspACI CCGC 2 cut(s) 64, 203
BspANI GGCC 3 cut(s) 37, 1076, 1110
BspCNI CTCAG 3 cut(s) 63, 624, 1122
BspLI GGNNCC 1 cut(s) 324
BspMAI CTGCAG 1 cut(s) 554
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 4 cut(s) 317, 330, 766, 920
BspTNI GGTCTC 1 cut(s) 1067
BsrDI GCAATG 2 cut(s) 366, 969
BsrFI RCCGGY 1 cut(s) 1076
BsrI ACTGG 5 cut(s) 355, 403, 511, 787, 836
BssAI RCCGGY 1 cut(s) 1076
BssECI CCNNGG 2 cut(s) 482, 1105
BssMI GATC 6 cut(s) 274, 286, 322, 498, 758, 925
BssT1I CCWWGG 1 cut(s) 482
Bst2UI CCWGG 1 cut(s) 251
Bst4CI ACNGT 4 cut(s) 217, 231, 385, 423
BstAPI GCANNNNNTGC 1 cut(s) 857
BstC8I GCNNGC 4 cut(s) 7, 203, 1041, 1089
BstDEI CTNAG 6 cut(s) 71, 182, 187, 632, 1071, 1130
BstF5I GGATG 1 cut(s) 471
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 6 cut(s) 277, 289, 325, 501, 761, 928
BstMAI GTCTC 1 cut(s) 1067
BstMBI GATC 6 cut(s) 274, 286, 322, 498, 758, 925
BstMCI CGRYCG 1 cut(s) 1111
BstMWI GCNNNNNNNGC 2 cut(s) 857, 1150
BstNI CCWGG 1 cut(s) 251
BstSCI CCNGG 3 cut(s) 249, 539, 1105
BstSFI CTRYAG 2 cut(s) 550, 600
BstSLI GKGCMC 1 cut(s) 264
BstV1I GCAGC 4 cut(s) 564, 640, 961, 1023
BstV2I GAAGAC 3 cut(s) 379, 386, 884
BstX2I RGATCY 2 cut(s) 322, 925
BstYI RGATCY 2 cut(s) 322, 925
BstZI CGGCCG 1 cut(s) 1108
Bsu36I CCTNAGG 1 cut(s) 1071
BsuI GTATCC 1 cut(s) 886
BsuRI GGCC 3 cut(s) 37, 1076, 1110
BtrI CACGTC 1 cut(s) 110
BtsCI GGATG 1 cut(s) 471
BtsIMutI CAGTG 3 cut(s) 504, 843, 997
Cac8I GCNNGC 4 cut(s) 7, 203, 1041, 1089
CfoI GCGC 1 cut(s) 154
Cfr10I RCCGGY 1 cut(s) 1076
Cfr13I GGNCC 1 cut(s) 428
CseI GACGC 1 cut(s) 43
Csp6I GTAC 4 cut(s) 247, 510, 712, 787
CspCI CAANNNNNGTGG 2 cut(s) 973, 1008
CviAII CATG 4 cut(s) 99, 223, 338, 557
CviQI GTAC 4 cut(s) 247, 510, 712, 787
DdeI CTNAG 6 cut(s) 71, 182, 187, 632, 1071, 1130
DpnI GATC 6 cut(s) 276, 288, 324, 500, 760, 927
DpnII GATC 6 cut(s) 274, 286, 322, 498, 758, 925
DriI GACNNNNNGTC 1 cut(s) 751
EaeI YGGCCR 1 cut(s) 1108
EagI CGGCCG 1 cut(s) 1108
Eam1105I GACNNNNNGTC 1 cut(s) 751
EclXI CGGCCG 1 cut(s) 1108
Eco130I CCWWGG 1 cut(s) 482
Eco31I GGTCTC 1 cut(s) 1067
Eco47I GGWCC 1 cut(s) 428
Eco52I CGGCCG 1 cut(s) 1108
Eco81I CCTNAGG 1 cut(s) 1071
EcoRI GAATTC 1 cut(s) 1160
EcoRII CCWGG 1 cut(s) 249
EcoT14I CCWWGG 1 cut(s) 482
ErhI CCWWGG 1 cut(s) 482
FaeI CATG 4 cut(s) 102, 226, 341, 560
FalI AAGNNNNNCTT 4 cut(s) 371, 403, 876, 908
FaqI GGGAC 1 cut(s) 96
FatI CATG 4 cut(s) 98, 222, 337, 556
FauI CCCGC 1 cut(s) 210
FauNDI CATATG 2 cut(s) 298, 433
FbaI TGATCA 1 cut(s) 274
FblI GTMKAC 3 cut(s) 192, 219, 744
Fnu4HI GCNGC 5 cut(s) 65, 553, 629, 975, 1037
FokI GGATG 1 cut(s) 458
Fsp4HI GCNGC 5 cut(s) 65, 553, 629, 975, 1037
FspBI CTAG 3 cut(s) 6, 899, 1044
GlaI GCGC 1 cut(s) 153
GluI GCNGC 5 cut(s) 65, 553, 629, 975, 1037
GsuI CTGGAG 3 cut(s) 633, 819, 1175
HaeIII GGCC 3 cut(s) 37, 1076, 1110
HapII CCGG 3 cut(s) 540, 1077, 1107
HgaI GACGC 1 cut(s) 43
HhaI GCGC 1 cut(s) 154
Hin1II CATG 4 cut(s) 102, 226, 341, 560
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 4 cut(s) 142, 341, 525, 572
HpaII CCGG 3 cut(s) 540, 1077, 1107
HphI GGTGA 1 cut(s) 322
Hpy166II GTNNAC 9 cut(s) 193, 220, 262, 453, 562, 745, 843, 920, 1022
Hpy188I TCNGA 4 cut(s) 22, 291, 797, 856
Hpy188III TCNNGA 5 cut(s) 376, 426, 650, 756, 1019
Hpy8I GTNNAC 9 cut(s) 193, 220, 262, 453, 562, 745, 843, 920, 1022
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 4 cut(s) 382, 599, 728, 959
HpyCH4III ACNGT 4 cut(s) 217, 231, 385, 423
HpyCH4IV ACGT 2 cut(s) 109, 307
HpyCH4V TGCA 4 cut(s) 262, 409, 552, 935
HpyF10VI GCNNNNNNNGC 2 cut(s) 857, 1150
HpyF3I CTNAG 6 cut(s) 71, 182, 187, 632, 1071, 1130
HpySE526I ACGT 2 cut(s) 109, 307
Hsp92II CATG 4 cut(s) 102, 226, 341, 560
HspAI GCGC 1 cut(s) 152
Ksp22I TGATCA 1 cut(s) 274
Kzo9I GATC 6 cut(s) 274, 286, 322, 498, 758, 925
LmnI GCTCC 1 cut(s) 1119
Lsp1109I GCAGC 4 cut(s) 564, 640, 961, 1023
LweI GCATC 2 cut(s) 847, 860
MaeI CTAG 3 cut(s) 6, 899, 1044
MaeII ACGT 2 cut(s) 109, 307
MaeIII GTNAC 3 cut(s) 778, 979, 1094
MalI GATC 6 cut(s) 276, 288, 324, 500, 760, 927
MboI GATC 6 cut(s) 274, 286, 322, 498, 758, 925
MboII GAAGA 9 cut(s) 379, 391, 493, 595, 784, 829, 884, 974, 1019
MflI RGATCY 2 cut(s) 322, 925
MhlI GDGCHC 2 cut(s) 77, 264
MluCI AATT 8 cut(s) 282, 467, 494, 694, 863, 942, 1031, 1160
MlyI GAGTC 1 cut(s) 335
MmeI TCCRAC 1 cut(s) 45
MnlI CCTC 5 cut(s) 486, 627, 694, 841, 1150
MseI TTAA 1 cut(s) 911
MspA1I CMGCKG 2 cut(s) 631, 764
MspI CCGG 3 cut(s) 540, 1077, 1107
MspR9I CCNGG 3 cut(s) 251, 541, 1107
MvaI CCWGG 1 cut(s) 251
MwoI GCNNNNNNNGC 2 cut(s) 857, 1150
NciI CCSGG 2 cut(s) 541, 1107
NdeI CATATG 2 cut(s) 298, 433
NdeII GATC 6 cut(s) 274, 286, 322, 498, 758, 925
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 4 cut(s) 102, 226, 341, 560
NlaIV GGNNCC 1 cut(s) 324
PfeI GAWTC 3 cut(s) 142, 525, 572
PkrI GCNGC 5 cut(s) 66, 554, 630, 976, 1038
PleI GAGTC 1 cut(s) 335
PpsI GAGTC 1 cut(s) 335
Psp6I CCWGG 1 cut(s) 249
PspGI CCWGG 1 cut(s) 249
PspN4I GGNNCC 1 cut(s) 324
PspPI GGNCC 1 cut(s) 428
PstI CTGCAG 1 cut(s) 554
PsuI RGATCY 2 cut(s) 322, 925
PvuII CAGCTG 2 cut(s) 631, 764
RsaI GTAC 4 cut(s) 248, 511, 713, 788
RsaNI GTAC 4 cut(s) 247, 510, 712, 787
SalI GTCGAC 1 cut(s) 218
SaqAI TTAA 1 cut(s) 911
SatI GCNGC 5 cut(s) 65, 553, 629, 975, 1037
Sau3AI GATC 6 cut(s) 274, 286, 322, 498, 758, 925
Sau96I GGNCC 1 cut(s) 428
ScaI AGTACT 1 cut(s) 713
SchI GAGTC 1 cut(s) 335
ScrFI CCNGG 3 cut(s) 251, 541, 1107
SduI GDGCHC 2 cut(s) 77, 264
SfaNI GCATC 2 cut(s) 847, 860
SfcI CTRYAG 2 cut(s) 550, 600
SinI GGWCC 1 cut(s) 428
Sse9I AATT 8 cut(s) 282, 467, 494, 694, 863, 942, 1031, 1160
SsiI CCGC 2 cut(s) 64, 203
SspMI CTAG 3 cut(s) 6, 899, 1044
StyD4I CCNGG 3 cut(s) 249, 539, 1105
StyI CCWWGG 1 cut(s) 482
TaaI ACNGT 4 cut(s) 217, 231, 385, 423
TaiI ACGT 2 cut(s) 112, 310
TaqI TCGA 3 cut(s) 219, 237, 806
TasI AATT 8 cut(s) 282, 467, 494, 694, 863, 942, 1031, 1160
TatI WGTACW 1 cut(s) 711
TauI GCSGC 1 cut(s) 67
TfiI GAWTC 3 cut(s) 142, 525, 572
Tru1I TTAA 1 cut(s) 911
Tru9I TTAA 1 cut(s) 911
TscAI CASTG 3 cut(s) 511, 843, 997
TseI GCWGC 4 cut(s) 552, 628, 974, 1036
TspDTI ATGAA 5 cut(s) 318, 450, 460, 479, 1152
TspRI CASTG 3 cut(s) 511, 843, 997
VneI GTGCAC 1 cut(s) 260
VpaK11BI GGWCC 1 cut(s) 428
XapI RAATTY 3 cut(s) 467, 863, 1160
XmiI GTMKAC 3 cut(s) 192, 219, 744
XspI CTAG 3 cut(s) 6, 899, 1044
ZrmI AGTACT 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.