Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0008037

Physical Location & Seq

Genomic Coordinates Forward (+)

14691397 .. 14694279

Gene Structure 2883 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1186000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1704 bp

ATGGCTCAAGCCGCGCGGCTTAACCTCCGACTGCAGAAGGAGCTCAAGCTCCTCCTCACTGATCCTCCGCCGGGCGCCTCTTTCCCTCTCCTCGCTTCCTCCTCCTCCCTCACCACCATCGACGCCCAGATCAAAGGTCCAGAAGGAACCGTGTACGCCGAAGGGTTCTTCAACATTAAGATTCAGATACCCGAGAGGTACCCGTTTCAGCCTCCGAGTGTGACATTTGCGACGCGAATTTACCACCCGAATATCGACACCGGAGGCCGGATTTGCCTCGACATTCTCAATCTTCCTCCGAAGGGGGCATGGCAACCATCATTGAACATTTCAACTGTGCTTACAAGCATTGGGTTGTTATTGAGTGAACCCAATCCAGATGATGGCCTCATGCGTGAAGCGAGTAGGGAATTCAAATACAACAGGCAAGCTTTCGACCAGAAAGCTCGATCTATGACTCAGAAATATGCCCAGTCTTCCGCTTCTGGGAGTGTTTGTGACTCTCAATCTCAACCTCAAAGCAGTGCAAATCCACTCACAGTGCTGCTGGAGGTTAAAGCTTCAATCCATGAGTCAAAACATGAAGCAGAGGAGTCTGCTGTGAGTAGTCATAAGAAGGTAATTGGGGGTAGCAGGAAGCTGTCACTGGAGTCTTCAGGCCAAACCCAGAAAAGGGATGTTTGCGGGAAAGGAAATGAAGTGCCAAAAGTGCAACTCTTTGCCTCTGATTTCGGAAACAAGATGGAAGTCAAAGGAACAGAAAAGTTGCTGGAAGATAAGTGCAGTCAGAGTCAGGAGAAGATATGTGGGAGCAGACGAAAACTATCGCTAGAAGCCCTCAGCCAACCCGAGAAGAAAAGTGATGGTATGGAGAATGTGGTGGCAAATCACCGTTCATCACTTAAACTGCAAAACCTTCCTGTGCCATCTTCAGGATCGGTTGCACTGCAATCTGGCAACAGTTACAAGCAAGGACTTCATCAGAGTCAAGATAGTAAATTGATAGATGACAACACAGGAGTGAGATCAAAGAGGCTGCGTCAATTCGAAAACAAGCAGTCATTGGCTTCTTTAAACACATCCCAAATATTTGATGAAAACAACGATAAAAAGTTCATTACCCCTCAACTGCCGCCCTCAGTGTCCCATTCCGATGCGTTATCGGAGCCTTTGTCAGCGGCACCAGCTCTTGACAGCATCAACTCACAACCACATAAAGATTTGATTGATGGAATGGGAAATGGCTCCGTTGGTATAAGTTGCAAGAAGCTTTGCTTAGCTGGCAAGAAGCTTTCGTTGGGATCTAGAGGCTCATCAGAGGCACATGGAAAGGCTAACAAGGAGAATGTGGCGCCAAAGTTGACTGAGAATCATACAGAATGTGAGATGGCTCATTTGCAAGAGAACAACAATAATAACACGCTTCCGCAGCCCCGGAGTATCTCTTCATATCCATCCAAGGCCCTTCCTGAGCAACAACAGAATTTGAAGCGGGATGAGAAGCAACAGTCTGATGAAGATTACAATTGTGGGATTGCTGCAGGGATTAATAAGCAAAAGGAAGGGAAAAAATTGGCAATGTGTGAGGCTGAGGCAGTGATTGTGTTGGATAGTGAAAGTAGTGATGAGGAAAGGAGTAGACCTGCGATGACTCTATCAGTGCTAGCAAGAAAGCGAATGGGAAAGTGGAGAGTAAGAACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0031625 GO:0032446 GO:0033554 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044464 GO:0050896 GO:0051716 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko03460 map03460

Pfam Domains

Protein Families

UQ_con

Protein Analysis

568

Amino Acids

61.93

Weight (kDa)

8.86

Isoelectric Point (pI)

60.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UQ_con	PF00179	10 - 151	6.5e-41	Ubiquitin-conjugating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008037)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24515 AT3G24515 AT3G24515 AT3G24515
fragaria_vesca	FvH4_1g04830 FvH4_1g04830 FvH4_4g32890
malus_domestica	MD08G1128600.v1.1 MD14G1109900.v1.1 MD15G1186000.v1.1
prunus_persica	Prupe.7G230900_v2.0.a1
pyrus_communis	pycom15g16670
rosa_chinensis	RchiOBHm_Chr2g0090471
rosa_laevigata	RLG00000016116
rosa_multiflora	Rmu_sc0004514.1_g000022 Rmu_sc0014034.1_g000001
rosa_roxburghii	Rroxscaffold_2G00151110
rosa_rugosa	Rorug02G0008200 Rorug02G0008300
rosa_samantha	Rh2AG053700 Rh2BG053200 Rh2CG055300 Rh2DG053900
rosa_wichuraiana	Rw2G004330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1651
Acc65I	GGTACC	1 cut(s)	198
AccB1I	GGYRCC	4 cut(s)	74, 198, 1180, 1351
AccB7I	CCANNNNNTGG	1 cut(s)	383
AccI	GTMKAC	1 cut(s)	1639
AccII	CGCG	3 cut(s)	14, 16, 235
AciI	CCGC	9 cut(s)	12, 16, 68, 480, 684, 1133, 1178, 1427, 1492
AclWI	GGATC	3 cut(s)	56, 943, 1309
AcsI	RAATTY	3 cut(s)	237, 410, 1483
AcuI	CTGAAG	2 cut(s)	639, 915
AcyI	GRCGYC	3 cut(s)	75, 123, 1352
AfaI	GTAC	2 cut(s)	155, 200
AfiI	CCNNNNNNNGG	8 cut(s)	71, 267, 302, 383, 486, 672, 673, 932
AgsI	TTSAA	6 cut(s)	172, 325, 333, 415, 564, 1489
AjuI	GAANNNNNNNTTGG	2 cut(s)	1280, 1312
AleI	CACNNNNGTG	1 cut(s)	1019
Alw21I	GWGCWC	1 cut(s)	45
AlwI	GGATC	3 cut(s)	56, 943, 1309
Ama87I	CYCGRG	2 cut(s)	191, 848
AoxI	GGCC	4 cut(s)	265, 385, 658, 1461
ApeKI	GCWGC	4 cut(s)	544, 1036, 1429, 1538
ApoI	RAATTY	3 cut(s)	237, 410, 1483
AseI	ATTAAT	1 cut(s)	1548
Asp718I	GGTACC	1 cut(s)	198
AspLEI	GCGC	3 cut(s)	16, 77, 1354
AspS9I	GGNCC	2 cut(s)	137, 1462
AsuC2I	CCSGG	2 cut(s)	72, 1435
AsuHPI	GGTGA	2 cut(s)	103, 881
AsuII	TTCGAA	1 cut(s)	1047
AsuNHI	GCTAGC	1 cut(s)	1663
AvaI	CYCGRG	2 cut(s)	191, 848
AvaII	GGWCC	1 cut(s)	137
BanI	GGYRCC	4 cut(s)	74, 198, 1180, 1351
BanII	GRGCYC	1 cut(s)	45
BbsI	GAAGAC	2 cut(s)	468, 645
Bbv12I	GWGCWC	1 cut(s)	45
BbvCI	CCTCAGC	2 cut(s)	839, 1590
BbvI	GCAGC	4 cut(s)	531, 1023, 1441, 1525
BccI	CCATC	9 cut(s)	125, 325, 377, 736, 857, 934, 1223, 1381, 1462
BcnI	CCSGG	2 cut(s)	72, 1435
BfaI	CTAG	3 cut(s)	830, 1305, 1664
BfmI	CTRYAG	2 cut(s)	32, 1539
BfoI	RGCGCY	2 cut(s)	78, 1355
BfuAI	ACCTGC	1 cut(s)	1651
BisI	GCNGC	8 cut(s)	12, 17, 545, 1037, 1133, 1179, 1430, 1539
BlpI	GCTNAGC	1 cut(s)	1276
BlsI	GCNGC	8 cut(s)	13, 18, 546, 1038, 1134, 1180, 1431, 1540
Bme1390I	CCNGG	2 cut(s)	72, 1435
Bme18I	GGWCC	1 cut(s)	137
BmeT110I	CYCGRG	2 cut(s)	191, 848
BmgT120I	GGNCC	2 cut(s)	137, 1462
BmiI	GGNNCC	7 cut(s)	76, 148, 200, 1167, 1182, 1246, 1353
BmrFI	CCNGG	2 cut(s)	72, 1435
BmrI	ACTGGG	1 cut(s)	466
BmsI	GCATC	2 cut(s)	1144, 1206
BmtI	GCTAGC	1 cut(s)	1667
BmuI	ACTGGG	1 cut(s)	466
BpiI	GAAGAC	2 cut(s)	468, 645
BpmI	CTGGAG	2 cut(s)	569, 668
Bpu10I	CCTNAGC	3 cut(s)	839, 1470, 1590
Bpu1102I	GCTNAGC	1 cut(s)	1276
Bpu14I	TTCGAA	1 cut(s)	1047
BpuEI	CTTGAG	1 cut(s)	29
BpuMI	CCSGG	2 cut(s)	72, 1435
BsaHI	GRCGYC	3 cut(s)	75, 123, 1352
BsaJI	CCNNGG	2 cut(s)	1433, 1458
BsaWI	WCCGGW	1 cut(s)	260
BsaXI	ACNNNNNCTCC	6 cut(s)	49, 79, 481, 511, 1157, 1187
Bsc4I	CCNNNNNNNGG	8 cut(s)	71, 267, 302, 383, 486, 672, 673, 932
Bse1I	ACTGG	2 cut(s)	472, 651
Bse3DI	GCAATG	1 cut(s)	1584
BseDI	CCNNGG	2 cut(s)	1433, 1458
BseGI	GGATG	4 cut(s)	682, 1079, 1454, 1501
BseLI	CCNNNNNNNGG	8 cut(s)	71, 267, 302, 383, 486, 672, 673, 932
BseMI	GCAATG	1 cut(s)	1584
BseMII	CTCAG	6 cut(s)	473, 853, 1152, 1356, 1461, 1581
BseNI	ACTGG	2 cut(s)	472, 651
BseRI	GAGGAG	6 cut(s)	41, 44, 80, 91, 94, 605
BseXI	GCAGC	4 cut(s)	531, 1023, 1441, 1525
BsgI	GTGCAG	1 cut(s)	802
Bsh1236I	CGCG	3 cut(s)	14, 16, 235
BshFI	GGCC	4 cut(s)	267, 387, 660, 1463
BshNI	GGYRCC	4 cut(s)	74, 198, 1180, 1351
BsiHKAI	GWGCWC	1 cut(s)	45
BsiHKCI	CYCGRG	2 cut(s)	191, 848
BsiSI	CCGG	4 cut(s)	71, 261, 268, 1435
BslFI	GGGAC	1 cut(s)	1129
BslI	CCNNNNNNNGG	8 cut(s)	71, 267, 302, 383, 486, 672, 673, 932
BsmFI	GGGAC	1 cut(s)	1129
BsnI	GGCC	4 cut(s)	267, 387, 660, 1463
BsoBI	CYCGRG	2 cut(s)	191, 848
Bsp119I	TTCGAA	1 cut(s)	1047
Bsp1286I	GDGCHC	1 cut(s)	45
Bsp143I	GATC	6 cut(s)	61, 129, 449, 935, 1025, 1301
Bsp1720I	GCTNAGC	1 cut(s)	1276
BspACI	CCGC	9 cut(s)	12, 16, 68, 480, 684, 1133, 1178, 1427, 1492
BspANI	GGCC	4 cut(s)	267, 387, 660, 1463
BspCNI	CTCAG	6 cut(s)	472, 852, 1151, 1357, 1462, 1582
BspFNI	CGCG	3 cut(s)	14, 16, 235
BspLI	GGNNCC	7 cut(s)	76, 148, 200, 1167, 1182, 1246, 1353
BspMAI	CTGCAG	2 cut(s)	36, 1543
BspMI	ACCTGC	1 cut(s)	1651
BspOI	GCTAGC	1 cut(s)	1667
BspPI	GGATC	3 cut(s)	56, 943, 1309
BspT104I	TTCGAA	1 cut(s)	1047
BspT107I	GGYRCC	4 cut(s)	74, 198, 1180, 1351
BsrDI	GCAATG	1 cut(s)	1584
BsrI	ACTGG	2 cut(s)	472, 651
BssECI	CCNNGG	2 cut(s)	1433, 1458
BssMI	GATC	6 cut(s)	61, 129, 449, 935, 1025, 1301
BssNI	GRCGYC	3 cut(s)	75, 123, 1352
BssT1I	CCWWGG	1 cut(s)	1458
Bst4CI	ACNGT	6 cut(s)	151, 337, 541, 893, 962, 1509
Bst6I	CTCTTC	1 cut(s)	1450
BstACI	GRCGYC	3 cut(s)	75, 123, 1352
BstBI	TTCGAA	1 cut(s)	1047
BstC8I	GCNNGC	3 cut(s)	429, 1282, 1665
BstDEI	CTNAG	7 cut(s)	459, 839, 1138, 1276, 1365, 1470, 1590
BstF5I	GGATG	4 cut(s)	682, 1079, 1454, 1501
BstFNI	CGCG	3 cut(s)	14, 16, 235
BstH2I	RGCGCY	2 cut(s)	78, 1355
BstHHI	GCGC	3 cut(s)	16, 77, 1354
BstKTI	GATC	6 cut(s)	64, 132, 452, 938, 1028, 1304
BstMBI	GATC	6 cut(s)	61, 129, 449, 935, 1025, 1301
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 40, 273, 709, 1184, 1281, 1429
BstSCI	CCNGG	2 cut(s)	70, 1433
BstSFI	CTRYAG	2 cut(s)	32, 1539
BstUI	CGCG	3 cut(s)	14, 16, 235
BstV1I	GCAGC	4 cut(s)	531, 1023, 1441, 1525
BstV2I	GAAGAC	2 cut(s)	468, 645
BstX2I	RGATCY	1 cut(s)	1301
BstYI	RGATCY	1 cut(s)	1301
BsuRI	GGCC	4 cut(s)	267, 387, 660, 1463
BtgZI	GCGATG	1 cut(s)	1661
BtsCI	GGATG	4 cut(s)	682, 1079, 1454, 1501
BtsI	GCAGTG	3 cut(s)	529, 944, 1602
BtsIMutI	CAGTG	8 cut(s)	57, 529, 546, 644, 944, 1146, 1602, 1665
BveI	ACCTGC	1 cut(s)	1651
Cac8I	GCNNGC	3 cut(s)	429, 1282, 1665
CfoI	GCGC	3 cut(s)	16, 77, 1354
Cfr13I	GGNCC	2 cut(s)	137, 1462
CseI	GACGC	3 cut(s)	131, 241, 1028
Csp6I	GTAC	2 cut(s)	154, 199
CviAII	CATG	5 cut(s)	309, 391, 569, 581, 1325
CviQI	GTAC	2 cut(s)	154, 199
DdeI	CTNAG	7 cut(s)	459, 839, 1138, 1276, 1365, 1470, 1590
DinI	GGCGCC	2 cut(s)	76, 1353
DpnI	GATC	6 cut(s)	63, 131, 451, 937, 1027, 1303
DpnII	GATC	6 cut(s)	61, 129, 449, 935, 1025, 1301
DraI	TTTAAA	1 cut(s)	1074
Eam1104I	CTCTTC	1 cut(s)	1450
EarI	CTCTTC	1 cut(s)	1450
EciI	GGCGGA	1 cut(s)	57
Ecl136II	GAGCTC	1 cut(s)	43
Eco130I	CCWWGG	1 cut(s)	1458
Eco24I	GRGCYC	1 cut(s)	45
Eco47I	GGWCC	1 cut(s)	137
Eco53kI	GAGCTC	1 cut(s)	43
Eco57I	CTGAAG	2 cut(s)	639, 915
Eco88I	CYCGRG	2 cut(s)	191, 848
EcoICRI	GAGCTC	1 cut(s)	43
EcoO109I	RGGNCCY	1 cut(s)	1462
EcoRI	GAATTC	1 cut(s)	410
EcoT14I	CCWWGG	1 cut(s)	1458
EcoT38I	GRGCYC	1 cut(s)	45
EgeI	GGCGCC	2 cut(s)	76, 1353
EheI	GGCGCC	2 cut(s)	76, 1353
ErhI	CCWWGG	1 cut(s)	1458
FaeI	CATG	5 cut(s)	312, 394, 572, 584, 1328
FalI	AAGNNNNNCTT	2 cut(s)	1259, 1291
FaqI	GGGAC	1 cut(s)	1129
FatI	CATG	5 cut(s)	308, 390, 568, 580, 1324
FauI	CCCGC	2 cut(s)	677, 1485
FblI	GTMKAC	1 cut(s)	1639
Fnu4HI	GCNGC	8 cut(s)	12, 17, 545, 1037, 1133, 1179, 1430, 1539
FokI	GGATG	4 cut(s)	689, 1066, 1441, 1508
FriOI	GRGCYC	1 cut(s)	45
Fsp4HI	GCNGC	8 cut(s)	12, 17, 545, 1037, 1133, 1179, 1430, 1539
FspBI	CTAG	3 cut(s)	830, 1305, 1664
GlaI	GCGC	3 cut(s)	15, 76, 1353
GluI	GCNGC	8 cut(s)	12, 17, 545, 1037, 1133, 1179, 1430, 1539
GsuI	CTGGAG	2 cut(s)	569, 668
HaeII	RGCGCY	2 cut(s)	78, 1355
HaeIII	GGCC	4 cut(s)	267, 387, 660, 1463
HapII	CCGG	4 cut(s)	71, 261, 268, 1435
HgaI	GACGC	3 cut(s)	131, 241, 1028
HhaI	GCGC	3 cut(s)	16, 77, 1354
Hin1I	GRCGYC	3 cut(s)	75, 123, 1352
Hin1II	CATG	5 cut(s)	312, 394, 572, 584, 1328
Hin6I	GCGC	3 cut(s)	14, 75, 1352
HinP1I	GCGC	3 cut(s)	14, 75, 1352
HincII	GTYRAC	1 cut(s)	1362
HindII	GTYRAC	1 cut(s)	1362
HindIII	AAGCTT	4 cut(s)	429, 558, 1268, 1289
HpaII	CCGG	4 cut(s)	71, 261, 268, 1435
HphI	GGTGA	2 cut(s)	103, 881
Hpy166II	GTNNAC	4 cut(s)	154, 368, 1362, 1640
Hpy188III	TCNNGA	8 cut(s)	140, 377, 794, 933, 989, 1190, 1305, 1469
Hpy8I	GTNNAC	4 cut(s)	154, 368, 1362, 1640
Hpy99I	CGWCG	2 cut(s)	125, 235
HpyAV	CCTTC	8 cut(s)	31, 137, 155, 295, 610, 926, 1475, 1556
HpyCH4III	ACNGT	6 cut(s)	151, 337, 541, 893, 962, 1509
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 40, 273, 709, 1184, 1281, 1429
HpyF3I	CTNAG	7 cut(s)	459, 839, 1138, 1276, 1365, 1470, 1590
Hsp92I	GRCGYC	3 cut(s)	75, 123, 1352
Hsp92II	CATG	5 cut(s)	312, 394, 572, 584, 1328
HspAI	GCGC	3 cut(s)	14, 75, 1352
KasI	GGCGCC	2 cut(s)	74, 1351
KpnI	GGTACC	1 cut(s)	202
Kzo9I	GATC	6 cut(s)	61, 129, 449, 935, 1025, 1301
LmnI	GCTCC	5 cut(s)	40, 54, 810, 1165, 1250
Lsp1109I	GCAGC	4 cut(s)	531, 1023, 1441, 1525
LweI	GCATC	2 cut(s)	1144, 1206
MaeI	CTAG	3 cut(s)	830, 1305, 1664
MaeIII	GTNAC	4 cut(s)	220, 497, 642, 962
MalI	GATC	6 cut(s)	63, 131, 451, 937, 1027, 1303
MboI	GATC	6 cut(s)	61, 129, 449, 935, 1025, 1301
MfeI	CAATTG	1 cut(s)	1525
MflI	RGATCY	1 cut(s)	1301
MhlI	GDGCHC	1 cut(s)	45
MluCI	AATT	8 cut(s)	237, 410, 621, 998, 1043, 1483, 1525, 1571
Mly113I	GGCGCC	2 cut(s)	75, 1352
MlyI	GAGTC	8 cut(s)	451, 494, 581, 602, 659, 799, 994, 1645
MmeI	TCCRAC	2 cut(s)	52, 1587
MseI	TTAA	6 cut(s)	21, 177, 555, 903, 1073, 1548
MslI	CAYNNNNRTG	2 cut(s)	1019, 1152
MspA1I	CMGCKG	1 cut(s)	1178
MspI	CCGG	4 cut(s)	71, 261, 268, 1435
MspR9I	CCNGG	2 cut(s)	72, 1435
MunI	CAATTG	1 cut(s)	1525
MvnI	CGCG	3 cut(s)	14, 16, 235
MwoI	GCNNNNNNNGC	7 cut(s)	11, 40, 273, 709, 1184, 1281, 1429
NarI	GGCGCC	2 cut(s)	75, 1352
NciI	CCSGG	2 cut(s)	72, 1435
NdeII	GATC	6 cut(s)	61, 129, 449, 935, 1025, 1301
NheI	GCTAGC	1 cut(s)	1663
NlaIII	CATG	5 cut(s)	312, 394, 572, 584, 1328
NlaIV	GGNNCC	7 cut(s)	76, 148, 200, 1167, 1182, 1246, 1353
NmuCI	GTSAC	3 cut(s)	220, 497, 642
NspV	TTCGAA	1 cut(s)	1047
OliI	CACNNNNGTG	1 cut(s)	1019
PfeI	GAWTC	2 cut(s)	181, 1369
PflMI	CCANNNNNTGG	1 cut(s)	383
PkrI	GCNGC	8 cut(s)	13, 18, 546, 1038, 1134, 1180, 1431, 1540
PleI	GAGTC	8 cut(s)	451, 494, 580, 601, 658, 798, 993, 1645
PluTI	GGCGCC	2 cut(s)	78, 1355
PpsI	GAGTC	8 cut(s)	451, 494, 580, 601, 658, 798, 993, 1645
PshBI	ATTAAT	1 cut(s)	1548
Psp124BI	GAGCTC	1 cut(s)	45
PspN4I	GGNNCC	7 cut(s)	76, 148, 200, 1167, 1182, 1246, 1353
PspPI	GGNCC	2 cut(s)	137, 1462
PstI	CTGCAG	2 cut(s)	36, 1543
PsuI	RGATCY	1 cut(s)	1301
RsaI	GTAC	2 cut(s)	155, 200
RsaNI	GTAC	2 cut(s)	154, 199
RseI	CAYNNNNRTG	2 cut(s)	1019, 1152
SacI	GAGCTC	1 cut(s)	45
SaqAI	TTAA	6 cut(s)	21, 177, 555, 903, 1073, 1548
SatI	GCNGC	8 cut(s)	12, 17, 545, 1037, 1133, 1179, 1430, 1539
Sau3AI	GATC	6 cut(s)	61, 129, 449, 935, 1025, 1301
Sau96I	GGNCC	2 cut(s)	137, 1462
SchI	GAGTC	8 cut(s)	451, 494, 581, 602, 659, 799, 994, 1645
ScrFI	CCNGG	2 cut(s)	72, 1435
SduI	GDGCHC	1 cut(s)	45
SfaNI	GCATC	2 cut(s)	1144, 1206
SfcI	CTRYAG	2 cut(s)	32, 1539
SfoI	GGCGCC	2 cut(s)	76, 1353
SfuI	TTCGAA	1 cut(s)	1047
SinI	GGWCC	1 cut(s)	137
SmiMI	CAYNNNNRTG	2 cut(s)	1019, 1152
SmlI	CTYRAG	2 cut(s)	6, 44
SmoI	CTYRAG	2 cut(s)	6, 44
Sse9I	AATT	8 cut(s)	237, 410, 621, 998, 1043, 1483, 1525, 1571
SsiI	CCGC	9 cut(s)	12, 16, 68, 480, 684, 1133, 1178, 1427, 1492
SspDI	GGCGCC	2 cut(s)	74, 1351
SspI	AATATT	1 cut(s)	1089
SspMI	CTAG	3 cut(s)	830, 1305, 1664
SstI	GAGCTC	1 cut(s)	45
StyD4I	CCNGG	2 cut(s)	70, 1433
StyI	CCWWGG	1 cut(s)	1458
TaaI	ACNGT	6 cut(s)	151, 337, 541, 893, 962, 1509
TaqI	TCGA	6 cut(s)	120, 255, 279, 435, 448, 1047
TasI	AATT	8 cut(s)	237, 410, 621, 998, 1043, 1483, 1525, 1571
TauI	GCSGC	4 cut(s)	14, 19, 1135, 1181
TfiI	GAWTC	2 cut(s)	181, 1369
Tru1I	TTAA	6 cut(s)	21, 177, 555, 903, 1073, 1548
Tru9I	TTAA	6 cut(s)	21, 177, 555, 903, 1073, 1548
TscAI	CASTG	8 cut(s)	64, 529, 546, 651, 951, 1146, 1602, 1665
TseFI	GTSAC	3 cut(s)	220, 497, 642
TseI	GCWGC	4 cut(s)	544, 1036, 1429, 1538
Tsp45I	GTSAC	3 cut(s)	220, 497, 642
TspDTI	ATGAA	8 cut(s)	597, 711, 885, 968, 1105, 1110, 1437, 1530
TspGWI	ACGGA	1 cut(s)	1237
TspRI	CASTG	8 cut(s)	64, 529, 546, 651, 951, 1146, 1602, 1665
Van91I	CCANNNNNTGG	1 cut(s)	383
VpaK11BI	GGWCC	1 cut(s)	137
VspI	ATTAAT	1 cut(s)	1548
XapI	RAATTY	3 cut(s)	237, 410, 1483
XbaI	TCTAGA	1 cut(s)	1304
XmiI	GTMKAC	1 cut(s)	1639
XspI	CTAG	3 cut(s)	830, 1305, 1664

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.