Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0013192

Physical Location & Seq

Genomic Coordinates Reverse (-)

25644254 .. 25647387

Gene Structure 3134 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1283700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 885 bp

ATGGCGCAAAGGTCCGTTCCGGCGCCGTTTCTGATGAAAACGTATCAACTTGTGGATGATCCGAGCTCGGATGACGTGATATCGTGGAATGAAAGTGGGACGACGTTTGTGGTTTGGAAGACAGTGGATTTTGCGAGGGACATGTTGCCTAAATATTTCAAGCACAACAATTTCTCAAGCTTCGTCCGCCAGCTTAATACCTATGGATTTCGAAAGACTGTGCCGGACAAATGGGAGTTCGCGAACGACAACTTCCAGCGAGGGCAGAAGGAGCTCCTGTCCGAGATCCGCCGCCGGAAATCTGTGACGTCAACGGCGGCGCACGCACCCCCCGGCGAAAAATCCGGAGTACCATCGACACCGTCGAACTCGGGGGAGGAGATGGCGTCGACGTCGACGTCGTCGCCGGACTCGAAGAACCCGGGGTCGGTGGAGACGGCGGCTCCGAGCCAGGTGGCGGATTTATCGGACGAGAACCAGAAGCTGAAGCGGGAGAACGAAAATTTGAACTCGGAGCTGGCGCAGACGAAGAAGCAGTGCGACGAGCTGGTGGGGTTTCTGGTGGAGCACATGAAGATGAAGCCCGATCAGATCAACCGCATCATCGGGCAGGGAATCTGTGGGCCCAGCGGCGATGATTTTGAAGCGGACGATCATGGTCTTGATCTGGATATGGAGGGTGGTGATGATGAGGAAGAAGAGGAGGGATTGAAGCTGTTTGGGGTTTGGGTGAAAGGAGAGGAGAAAATGAAGGGGAAGAAAACGGGGCGATATGAGATATTGGGGGTTGGTGGTGGCGGTGCCGGCGGGCCCCGCGCGAAGAGGATGAAGGGAGGGGTGGAGTTTCACGCGCCGATGATGAGGACCGGGAAGGTGTGCAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HSF_DNA-bind

Protein Analysis

295

Amino Acids

32.42

Weight (kDa)

5.44

Isoelectric Point (pI)

37.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSF_DNA-bind	PF00447	10 - 99	2.5e-31	HSF-type DNA-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013192)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36990
fragaria_vesca	FvH4_1g16030
malus_domestica	MD02G1171800.v1.1 MD15G1283700.v1.1
prunus_persica	Prupe.7G133600_v2.0.a1
pyrus_communis	pycom02g13850 pycom15g24670
rosa_chinensis	RchiOBHm_Chr2g0105601
rosa_laevigata	RLG00000017444
rosa_multiflora	Rmu_co8474487.1_g000001 Rmu_sc0001051.1_g000029
rosa_roxburghii	Rroxscaffold_2G00138040
rosa_rugosa	Rorug02G0129000
rosa_samantha	Rh2AG180500 Rh2BG189300 Rh2CG184700 Rh2DG186200
rosa_wichuraiana	Rw2G014110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	397
AatII	GACGTC	3 cut(s)	311, 395, 401
AccB1I	GGYRCC	2 cut(s)	22, 800
AccI	GTMKAC	2 cut(s)	389, 395
AccII	CGCG	4 cut(s)	242, 816, 818, 851
AccIII	TCCGGA	1 cut(s)	344
AclWI	GGATC	2 cut(s)	53, 280
AcsI	RAATTY	1 cut(s)	502
AcuI	CTGAAG	1 cut(s)	506
AcyI	GRCGYC	5 cut(s)	23, 308, 386, 392, 398
AfaI	GTAC	1 cut(s)	351
AfiI	CCNNNNNNNGG	2 cut(s)	427, 457
AflIII	ACRYGT	1 cut(s)	141
AgsI	TTSAA	4 cut(s)	160, 508, 644, 712
AjiI	CACGTC	1 cut(s)	76
AjnI	CCWGG	1 cut(s)	450
AluBI	AGCT	8 cut(s)	66, 180, 193, 274, 484, 517, 547, 715
AluI	AGCT	8 cut(s)	66, 180, 193, 274, 484, 517, 547, 715
Alw21I	GWGCWC	3 cut(s)	68, 276, 570
Alw26I	GTCTC	1 cut(s)	428
AlwI	GGATC	2 cut(s)	53, 280
AlwNI	CAGNNNCTG	1 cut(s)	484
Ama87I	CYCGRG	2 cut(s)	370, 421
Aor13HI	TCCGGA	1 cut(s)	344
AoxI	GGCC	2 cut(s)	623, 809
ApaI	GGGCCC	2 cut(s)	627, 813
ApoI	RAATTY	1 cut(s)	502
AspLEI	GCGC	6 cut(s)	7, 25, 322, 523, 818, 853
AspS9I	GGNCC	6 cut(s)	12, 623, 624, 809, 810, 864
AsuC2I	CCSGG	4 cut(s)	333, 422, 423, 868
AsuHPI	GGTGA	2 cut(s)	695, 742
AsuII	TTCGAA	1 cut(s)	211
AvaI	CYCGRG	2 cut(s)	370, 421
AvaII	GGWCC	2 cut(s)	12, 864
BaeGI	GKGCMC	2 cut(s)	627, 813
BanI	GGYRCC	2 cut(s)	22, 800
BanII	GRGCYC	4 cut(s)	68, 276, 627, 813
BbsI	GAAGAC	1 cut(s)	125
Bbv12I	GWGCWC	3 cut(s)	68, 276, 570
BccI	CCATC	2 cut(s)	361, 376
BceAI	ACGGC	3 cut(s)	10, 330, 453
BciT130I	CCWGG	1 cut(s)	452
BcnI	CCSGG	4 cut(s)	333, 422, 423, 868
BcoDI	GTCTC	1 cut(s)	428
BfaI	CTAG	1 cut(s)	883
BfoI	RGCGCY	1 cut(s)	26
BisI	GCNGC	4 cut(s)	292, 318, 441, 631
BlsI	GCNGC	4 cut(s)	293, 319, 442, 632
Bme1390I	CCNGG	5 cut(s)	333, 422, 423, 452, 868
Bme18I	GGWCC	2 cut(s)	12, 864
BmeT110I	CYCGRG	2 cut(s)	370, 421
BmgBI	CACGTC	1 cut(s)	76
BmgT120I	GGNCC	6 cut(s)	12, 623, 624, 809, 810, 864
BmiI	GGNNCC	6 cut(s)	24, 444, 625, 802, 811, 812
BmrFI	CCNGG	5 cut(s)	333, 422, 423, 452, 868
BmsI	GCATC	1 cut(s)	609
BpiI	GAAGAC	1 cut(s)	125
Bpu14I	TTCGAA	1 cut(s)	211
BpuEI	CTTGAG	1 cut(s)	160
BpuMI	CCSGG	4 cut(s)	333, 422, 423, 868
BsaHI	GRCGYC	5 cut(s)	23, 308, 386, 392, 398
BsaJI	CCNNGG	3 cut(s)	331, 421, 422
BsaWI	WCCGGW	1 cut(s)	344
BsaXI	ACNNNNNCTCC	6 cut(s)	263, 293, 371, 401, 427, 457
Bsc4I	CCNNNNNNNGG	2 cut(s)	427, 457
Bse118I	RCCGGY	1 cut(s)	803
BseAI	TCCGGA	1 cut(s)	344
BseBI	CCWGG	1 cut(s)	452
BseDI	CCNNGG	3 cut(s)	331, 421, 422
BseGI	GGATG	3 cut(s)	61, 76, 831
BseLI	CCNNNNNNNGG	2 cut(s)	427, 457
BseRI	GAGGAG	3 cut(s)	392, 716, 755
BseSI	GKGCMC	2 cut(s)	627, 813
BseYI	CCCAGC	1 cut(s)	626
Bsh1236I	CGCG	4 cut(s)	242, 816, 818, 851
BshFI	GGCC	2 cut(s)	625, 811
BshNI	GGYRCC	2 cut(s)	22, 800
BsiHKAI	GWGCWC	3 cut(s)	68, 276, 570
BsiHKCI	CYCGRG	2 cut(s)	370, 421
BsiSI	CCGG	9 cut(s)	20, 224, 295, 333, 345, 407, 422, 804, 867
BslFI	GGGAC	2 cut(s)	112, 152
BslI	CCNNNNNNNGG	2 cut(s)	427, 457
BsmAI	GTCTC	1 cut(s)	428
BsmBI	CGTCTC	1 cut(s)	428
BsmFI	GGGAC	2 cut(s)	112, 152
BsnI	GGCC	2 cut(s)	625, 811
BsoBI	CYCGRG	2 cut(s)	370, 421
Bsp119I	TTCGAA	1 cut(s)	211
Bsp120I	GGGCCC	2 cut(s)	623, 809
Bsp1286I	GDGCHC	5 cut(s)	68, 276, 570, 627, 813
Bsp13I	TCCGGA	1 cut(s)	344
Bsp143I	GATC	6 cut(s)	58, 285, 586, 591, 652, 664
Bsp68I	TCGCGA	1 cut(s)	242
BspANI	GGCC	2 cut(s)	625, 811
BspEI	TCCGGA	1 cut(s)	344
BspFNI	CGCG	4 cut(s)	242, 816, 818, 851
BspLI	GGNNCC	6 cut(s)	24, 444, 625, 802, 811, 812
BspPI	GGATC	2 cut(s)	53, 280
BspT104I	TTCGAA	1 cut(s)	211
BspT107I	GGYRCC	2 cut(s)	22, 800
BsrFI	RCCGGY	1 cut(s)	803
BssAI	RCCGGY	1 cut(s)	803
BssECI	CCNNGG	3 cut(s)	331, 421, 422
BssMI	GATC	6 cut(s)	58, 285, 586, 591, 652, 664
BssNI	GRCGYC	5 cut(s)	23, 308, 386, 392, 398
Bst2UI	CCWGG	1 cut(s)	452
Bst4CI	ACNGT	3 cut(s)	124, 220, 363
Bst6I	CTCTTC	2 cut(s)	693, 815
BstACI	GRCGYC	5 cut(s)	23, 308, 386, 392, 398
BstBI	TTCGAA	1 cut(s)	211
BstC8I	GCNNGC	5 cut(s)	191, 324, 519, 805, 809
BstF5I	GGATG	3 cut(s)	61, 76, 831
BstFNI	CGCG	4 cut(s)	242, 816, 818, 851
BstH2I	RGCGCY	1 cut(s)	26
BstHHI	GCGC	6 cut(s)	7, 25, 322, 523, 818, 853
BstKTI	GATC	6 cut(s)	61, 288, 589, 594, 655, 667
BstMAI	GTCTC	1 cut(s)	428
BstMBI	GATC	6 cut(s)	58, 285, 586, 591, 652, 664
BstMWI	GCNNNNNNNGC	5 cut(s)	186, 271, 323, 804, 813
BstNI	CCWGG	1 cut(s)	452
BstNSI	RCATGY	1 cut(s)	145
BstSCI	CCNGG	5 cut(s)	331, 420, 421, 450, 866
BstSLI	GKGCMC	2 cut(s)	627, 813
BstUI	CGCG	4 cut(s)	242, 816, 818, 851
BstV2I	GAAGAC	1 cut(s)	125
BstX2I	RGATCY	1 cut(s)	285
BstYI	RGATCY	1 cut(s)	285
BsuRI	GGCC	2 cut(s)	625, 811
BtgZI	GCGATG	1 cut(s)	648
BtrI	CACGTC	1 cut(s)	76
BtsCI	GGATG	3 cut(s)	61, 76, 831
BtsI	GCAGTG	1 cut(s)	542
BtsIMutI	CAGTG	2 cut(s)	129, 542
BtuMI	TCGCGA	1 cut(s)	242
Cac8I	GCNNGC	5 cut(s)	191, 324, 519, 805, 809
CaiI	CAGNNNCTG	1 cut(s)	484
CfoI	GCGC	6 cut(s)	7, 25, 322, 523, 818, 853
Cfr10I	RCCGGY	1 cut(s)	803
Cfr13I	GGNCC	6 cut(s)	12, 623, 624, 809, 810, 864
Cfr9I	CCCGGG	1 cut(s)	421
CseI	GACGC	1 cut(s)	375
Csp6I	GTAC	1 cut(s)	350
CviAII	CATG	3 cut(s)	142, 571, 656
CviQI	GTAC	1 cut(s)	350
DinI	GGCGCC	1 cut(s)	24
DpnI	GATC	6 cut(s)	60, 287, 588, 593, 654, 666
DpnII	GATC	6 cut(s)	58, 285, 586, 591, 652, 664
DrdI	GACNNNNNNGTC	1 cut(s)	397
DseDI	GACNNNNNNGTC	1 cut(s)	397
Eam1104I	CTCTTC	2 cut(s)	693, 815
EarI	CTCTTC	2 cut(s)	693, 815
EciI	GGCGGA	3 cut(s)	176, 278, 473
Ecl136II	GAGCTC	2 cut(s)	66, 274
Eco24I	GRGCYC	4 cut(s)	68, 276, 627, 813
Eco32I	GATATC	1 cut(s)	81
Eco47I	GGWCC	2 cut(s)	12, 864
Eco53kI	GAGCTC	2 cut(s)	66, 274
Eco57I	CTGAAG	1 cut(s)	506
Eco88I	CYCGRG	2 cut(s)	370, 421
EcoICRI	GAGCTC	2 cut(s)	66, 274
EcoO109I	RGGNCCY	1 cut(s)	810
EcoRII	CCWGG	1 cut(s)	450
EcoRV	GATATC	1 cut(s)	81
EcoT38I	GRGCYC	4 cut(s)	68, 276, 627, 813
EgeI	GGCGCC	1 cut(s)	24
EheI	GGCGCC	1 cut(s)	24
Esp3I	CGTCTC	1 cut(s)	428
FaeI	CATG	3 cut(s)	145, 574, 659
FaiI	YATR	6 cut(s)	143, 204, 572, 657, 674, 774
FaqI	GGGAC	2 cut(s)	112, 152
FatI	CATG	3 cut(s)	141, 570, 655
FauI	CCCGC	3 cut(s)	483, 800, 821
FblI	GTMKAC	2 cut(s)	389, 395
Fnu4HI	GCNGC	4 cut(s)	292, 318, 441, 631
FokI	GGATG	3 cut(s)	68, 83, 838
FriOI	GRGCYC	4 cut(s)	68, 276, 627, 813
Fsp4HI	GCNGC	4 cut(s)	292, 318, 441, 631
FspBI	CTAG	1 cut(s)	883
GlaI	GCGC	6 cut(s)	6, 24, 321, 522, 817, 852
GluI	GCNGC	4 cut(s)	292, 318, 441, 631
GsaI	CCCAGC	1 cut(s)	630
HaeII	RGCGCY	1 cut(s)	26
HaeIII	GGCC	2 cut(s)	625, 811
HapII	CCGG	9 cut(s)	20, 224, 295, 333, 345, 407, 422, 804, 867
HgaI	GACGC	1 cut(s)	375
HhaI	GCGC	6 cut(s)	7, 25, 322, 523, 818, 853
Hin1I	GRCGYC	5 cut(s)	23, 308, 386, 392, 398
Hin1II	CATG	3 cut(s)	145, 574, 659
Hin6I	GCGC	6 cut(s)	5, 23, 320, 521, 816, 851
HinP1I	GCGC	6 cut(s)	5, 23, 320, 521, 816, 851
HincII	GTYRAC	3 cut(s)	312, 390, 396
HindII	GTYRAC	3 cut(s)	312, 390, 396
HindIII	AAGCTT	1 cut(s)	178
HinfI	GANTC	2 cut(s)	410, 615
HpaII	CCGG	9 cut(s)	20, 224, 295, 333, 345, 407, 422, 804, 867
HphI	GGTGA	2 cut(s)	695, 742
Hpy166II	GTNNAC	3 cut(s)	312, 390, 396
Hpy188I	TCNGA	8 cut(s)	33, 63, 70, 283, 447, 469, 514, 591
Hpy188III	TCNNGA	4 cut(s)	241, 345, 662, 668
Hpy8I	GTNNAC	3 cut(s)	312, 390, 396
Hpy99I	CGWCG	9 cut(s)	106, 367, 391, 394, 397, 400, 403, 406, 545
HpyAV	CCTTC	4 cut(s)	262, 745, 823, 865
HpyCH4III	ACNGT	3 cut(s)	124, 220, 363
HpyCH4IV	ACGT	6 cut(s)	41, 75, 104, 308, 392, 398
HpyCH4V	TGCA	1 cut(s)	879
HpyF10VI	GCNNNNNNNGC	5 cut(s)	186, 271, 323, 804, 813
HpySE526I	ACGT	6 cut(s)	41, 75, 104, 308, 392, 398
Hsp92I	GRCGYC	5 cut(s)	23, 308, 386, 392, 398
Hsp92II	CATG	3 cut(s)	145, 574, 659
HspAI	GCGC	6 cut(s)	5, 23, 320, 521, 816, 851
KasI	GGCGCC	1 cut(s)	22
Kpn2I	TCCGGA	1 cut(s)	344
KroI	GCCGGC	1 cut(s)	803
KroNI	GCCGGC	1 cut(s)	805
Kzo9I	GATC	6 cut(s)	58, 285, 586, 591, 652, 664
LmnI	GCTCC	5 cut(s)	271, 279, 448, 514, 565
LweI	GCATC	1 cut(s)	609
MaeI	CTAG	1 cut(s)	883
MaeII	ACGT	6 cut(s)	41, 75, 104, 308, 392, 398
MaeIII	GTNAC	1 cut(s)	304
MalI	GATC	6 cut(s)	60, 287, 588, 593, 654, 666
MboI	GATC	6 cut(s)	58, 285, 586, 591, 652, 664
MboII	GAAGA	8 cut(s)	130, 427, 541, 586, 707, 710, 769, 832
MflI	RGATCY	1 cut(s)	285
MhlI	GDGCHC	5 cut(s)	68, 276, 570, 627, 813
MluCI	AATT	2 cut(s)	169, 502
Mly113I	GGCGCC	1 cut(s)	23
MlyI	GAGTC	1 cut(s)	404
MroI	TCCGGA	1 cut(s)	344
MroNI	GCCGGC	1 cut(s)	803
MseI	TTAA	1 cut(s)	195
MslI	CAYNNNNRTG	1 cut(s)	575
MspA1I	CMGCKG	1 cut(s)	630
MspI	CCGG	9 cut(s)	20, 224, 295, 333, 345, 407, 422, 804, 867
MspR9I	CCNGG	5 cut(s)	333, 422, 423, 452, 868
MvaI	CCWGG	1 cut(s)	452
MvnI	CGCG	4 cut(s)	242, 816, 818, 851
MwoI	GCNNNNNNNGC	5 cut(s)	186, 271, 323, 804, 813
NaeI	GCCGGC	1 cut(s)	805
NarI	GGCGCC	1 cut(s)	23
NciI	CCSGG	4 cut(s)	333, 422, 423, 868
NdeII	GATC	6 cut(s)	58, 285, 586, 591, 652, 664
NgoMIV	GCCGGC	1 cut(s)	803
NlaIII	CATG	3 cut(s)	145, 574, 659
NlaIV	GGNNCC	6 cut(s)	24, 444, 625, 802, 811, 812
NmuCI	GTSAC	1 cut(s)	304
NruI	TCGCGA	1 cut(s)	242
NspI	RCATGY	1 cut(s)	145
NspV	TTCGAA	1 cut(s)	211
PciI	ACATGT	1 cut(s)	141
PcsI	WCGNNNNNNNCGW	5 cut(s)	362, 395, 398, 410, 443
PdiI	GCCGGC	1 cut(s)	805
PfeI	GAWTC	1 cut(s)	615
PflFI	GACNNNGTC	2 cut(s)	361, 400
PkrI	GCNGC	4 cut(s)	293, 319, 442, 632
PleI	GAGTC	1 cut(s)	404
PluTI	GGCGCC	1 cut(s)	26
PpsI	GAGTC	1 cut(s)	404
PscI	ACATGT	1 cut(s)	141
Psp124BI	GAGCTC	2 cut(s)	68, 276
Psp6I	CCWGG	1 cut(s)	450
PspFI	CCCAGC	1 cut(s)	626
PspGI	CCWGG	1 cut(s)	450
PspN4I	GGNNCC	6 cut(s)	24, 444, 625, 802, 811, 812
PspOMI	GGGCCC	2 cut(s)	623, 809
PspPI	GGNCC	6 cut(s)	12, 623, 624, 809, 810, 864
PstNI	CAGNNNCTG	1 cut(s)	484
PsuI	RGATCY	1 cut(s)	285
PsyI	GACNNNGTC	2 cut(s)	361, 400
RruI	TCGCGA	1 cut(s)	242
RsaI	GTAC	1 cut(s)	351
RsaNI	GTAC	1 cut(s)	350
RseI	CAYNNNNRTG	1 cut(s)	575
SacI	GAGCTC	2 cut(s)	68, 276
SalI	GTCGAC	2 cut(s)	388, 394
SaqAI	TTAA	1 cut(s)	195
SatI	GCNGC	4 cut(s)	292, 318, 441, 631
Sau3AI	GATC	6 cut(s)	58, 285, 586, 591, 652, 664
Sau96I	GGNCC	6 cut(s)	12, 623, 624, 809, 810, 864
SchI	GAGTC	1 cut(s)	404
ScrFI	CCNGG	5 cut(s)	333, 422, 423, 452, 868
SduI	GDGCHC	5 cut(s)	68, 276, 570, 627, 813
SfaNI	GCATC	1 cut(s)	609
SfoI	GGCGCC	1 cut(s)	24
SfuI	TTCGAA	1 cut(s)	211
SgrDI	CGTCGACG	2 cut(s)	388, 394
SinI	GGWCC	2 cut(s)	12, 864
SmaI	CCCGGG	1 cut(s)	423
SmiMI	CAYNNNNRTG	1 cut(s)	575
SmlI	CTYRAG	1 cut(s)	175
SmoI	CTYRAG	1 cut(s)	175
Sse9I	AATT	2 cut(s)	169, 502
SspDI	GGCGCC	1 cut(s)	22
SspI	AATATT	1 cut(s)	155
SspMI	CTAG	1 cut(s)	883
SstI	GAGCTC	2 cut(s)	68, 276
StyD4I	CCNGG	5 cut(s)	331, 420, 421, 450, 866
TaaI	ACNGT	3 cut(s)	124, 220, 363
TaiI	ACGT	6 cut(s)	44, 78, 107, 311, 395, 401
TaqI	TCGA	6 cut(s)	211, 356, 365, 389, 395, 413
TasI	AATT	2 cut(s)	169, 502
TauI	GCSGC	4 cut(s)	294, 320, 443, 633
TfiI	GAWTC	1 cut(s)	615
Tru1I	TTAA	1 cut(s)	195
Tru9I	TTAA	1 cut(s)	195
TscAI	CASTG	2 cut(s)	129, 542
TseFI	GTSAC	1 cut(s)	304
Tsp45I	GTSAC	1 cut(s)	304
TspDTI	ATGAA	6 cut(s)	50, 105, 587, 593, 764, 842
TspGWI	ACGGA	1 cut(s)	4
TspMI	CCCGGG	1 cut(s)	421
TspRI	CASTG	2 cut(s)	129, 542
Tth111I	GACNNNGTC	2 cut(s)	361, 400
VpaK11BI	GGWCC	2 cut(s)	12, 864
XapI	RAATTY	1 cut(s)	502
XceI	RCATGY	1 cut(s)	145
XmaI	CCCGGG	1 cut(s)	421
XmiI	GTMKAC	2 cut(s)	389, 395
XspI	CTAG	1 cut(s)	883
ZraI	GACGTC	3 cut(s)	309, 393, 399

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.