pycom02g13850

heat shock factor protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0013192
Physical Location & Seq
Reverse (-)
10436227 .. 10438451
2225 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g13850.1

Sequence Viewer

Length: 885 bp
ATGGCGCAAAGGTCCGTTCCGGCGCCGTTTCTGACGAAGACGTATCTACTGGTGGATGATCCGAGCTTGGACGACGTGATATCATGGAATGAAAGTGGGACGACGTTTGTGGTTTGGAAGACTCTGGATTTTGCAAGGGACTTGTTGCCTAAATATTTCAAACACAACAATTTCTCAAGCTTCGTCCGCCAGCTCAATACCTATGGATTTCGAAAGACCGTGCCGGACAAATGGGAGTTCGCGAACGAGAATTTCCAGCGAGGGCAGAAGGAGCTCCTGTCCGAGATCCGCCGCCGAAAACCGGTGACATCAACGGCGGCCCAGGCAGCGCTAGGTGAAAAATCTGGAGGGCCATCGACACCGTCGAACTCAGGGGAGGAGCTGGCGTCGACGTCGACGTCGTTGCCGGACTCGAAGAATCCGGGGTCGGTGGAGACGGCGACGCTGAGCCAGGTGGCGGATTTATCGGACGAGAACAAGAAGCTGAAGCGGGAGAACGAGAATCTGAACTCGGAGCTGGCGCAGACAAAGAAGCAGTGCGACGAGCTGGTGGGGTTTCTGGTGGAGTACGTGAAGATGGGGCCCGAACAGATCAACCGCATCATTGGGCGTGGAAGCTATGGGCCCACCCGCGATGATTTTGAACCGGACGATCGTGGTCTTGCTCTTGATATGGACGGTGGTGATGATGACGAAGAAGAGAAGGAAGGACTGAAGCTGTTTGGGGTTTGGGTGAAGGGGGACGAGAAAATGAAGGGGAAGAAAACGGGGCGAGATGAGAAATTCGGTGTTTGTGGTGTCGGTGGTGTTCCCCGCGCGAAGAGGATGAAGGGCGGCGTGGATTTTCACGCGCCGATGATGAGGAGCGGGAAGGTGTGTAACTAG

Protein Analysis

295

Amino Acids

32.64

Weight (kDa)

5.93

Isoelectric Point (pI)

32.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 10 - 99 1.9e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013192)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36990
fragaria_vesca FvH4_1g16030
malus_domestica MD02G1171800.v1.1 MD15G1283700.v1.1
prunus_persica Prupe.7G133600_v2.0.a1
pyrus_communis pycom02g13850 pycom15g24670
rosa_chinensis RchiOBHm_Chr2g0105601
rosa_laevigata RLG00000017444
rosa_multiflora Rmu_co8474487.1_g000001 Rmu_sc0001051.1_g000029
rosa_roxburghii Rroxscaffold_2G00138040
rosa_rugosa Rorug02G0129000
rosa_samantha Rh2AG180500 Rh2BG189300 Rh2CG184700 Rh2DG186200
rosa_wichuraiana Rw2G014110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 397
AatII GACGTC 2 cut(s) 395, 401
AccB1I GGYRCC 1 cut(s) 22
AccBSI CCGCTC 1 cut(s) 867
AccI GTMKAC 2 cut(s) 389, 395
AccII CGCG 5 cut(s) 242, 633, 816, 818, 851
AclWI GGATC 2 cut(s) 53, 280
AcsI RAATTY 2 cut(s) 250, 782
AcuI CTGAAG 2 cut(s) 506, 734
AcyI GRCGYC 4 cut(s) 23, 386, 392, 398
AfaI GTAC 1 cut(s) 569
AfeI AGCGCT 1 cut(s) 330
AfiI CCNNNNNNNGG 2 cut(s) 301, 457
AgeI ACCGGT 1 cut(s) 301
AgsI TTSAA 2 cut(s) 160, 644
AjiI CACGTC 1 cut(s) 76
AjnI CCWGG 2 cut(s) 321, 450
Alw21I GWGCWC 1 cut(s) 276
Alw26I GTCTC 1 cut(s) 428
AlwI GGATC 2 cut(s) 53, 280
Aor51HI AGCGCT 1 cut(s) 330
AoxI GGCC 4 cut(s) 318, 350, 581, 623
ApaI GGGCCC 2 cut(s) 585, 627
ApeKI GCWGC 1 cut(s) 326
ApoI RAATTY 2 cut(s) 250, 782
AsiGI ACCGGT 1 cut(s) 301
AspLEI GCGC 6 cut(s) 7, 25, 331, 523, 818, 853
AspS9I GGNCC 7 cut(s) 12, 319, 350, 581, 582, 623, 624
AsuC2I CCSGG 1 cut(s) 423
AsuHPI GGTGA 4 cut(s) 316, 347, 695, 745
AsuII TTCGAA 1 cut(s) 211
AvaII GGWCC 1 cut(s) 12
BaeGI GKGCMC 2 cut(s) 585, 627
BanI GGYRCC 1 cut(s) 22
BanII GRGCYC 3 cut(s) 276, 585, 627
BbsI GAAGAC 2 cut(s) 44, 125
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 1 cut(s) 338
BccI CCATC 2 cut(s) 361, 571
BceAI ACGGC 3 cut(s) 10, 330, 453
BcgI CGANNNNNNTGC 2 cut(s) 385, 419
BciT130I CCWGG 2 cut(s) 323, 452
BcnI CCSGG 1 cut(s) 423
BcoDI GTCTC 1 cut(s) 428
BfaI CTAG 2 cut(s) 332, 883
BfoI RGCGCY 2 cut(s) 26, 332
BisI GCNGC 4 cut(s) 292, 318, 327, 835
BlpI GCTNAGC 1 cut(s) 446
BlsI GCNGC 4 cut(s) 293, 319, 328, 836
Bme1390I CCNGG 3 cut(s) 323, 423, 452
Bme18I GGWCC 1 cut(s) 12
BmgBI CACGTC 1 cut(s) 76
BmgT120I GGNCC 7 cut(s) 12, 319, 350, 581, 582, 623, 624
BmiI GGNNCC 4 cut(s) 24, 582, 583, 625
BmrFI CCNGG 3 cut(s) 323, 423, 452
BmsI GCATC 1 cut(s) 609
BpiI GAAGAC 2 cut(s) 44, 125
BpmI CTGGAG 1 cut(s) 366
Bpu1102I GCTNAGC 1 cut(s) 446
Bpu14I TTCGAA 1 cut(s) 211
BpuEI CTTGAG 1 cut(s) 160
BpuMI CCSGG 1 cut(s) 423
BsaAI YACGTR 1 cut(s) 571
BsaHI GRCGYC 4 cut(s) 23, 386, 392, 398
BsaJI CCNNGG 2 cut(s) 321, 422
BsaWI WCCGGW 2 cut(s) 301, 646
BsaXI ACNNNNNCTCC 4 cut(s) 263, 293, 371, 401
Bsc4I CCNNNNNNNGG 2 cut(s) 301, 457
Bse118I RCCGGY 1 cut(s) 301
Bse1I ACTGG 1 cut(s) 54
BseBI CCWGG 2 cut(s) 323, 452
BseDI CCNNGG 2 cut(s) 321, 422
BseGI GGATG 2 cut(s) 61, 831
BseLI CCNNNNNNNGG 2 cut(s) 301, 457
BseMII CTCAG 2 cut(s) 384, 437
BseNI ACTGG 1 cut(s) 54
BseRI GAGGAG 2 cut(s) 392, 877
BseSI GKGCMC 2 cut(s) 585, 627
BseXI GCAGC 1 cut(s) 338
Bsh1236I CGCG 5 cut(s) 242, 633, 816, 818, 851
Bsh1285I CGRYCG 1 cut(s) 655
BshFI GGCC 4 cut(s) 320, 352, 583, 625
BshNI GGYRCC 1 cut(s) 22
BshTI ACCGGT 1 cut(s) 301
BsiEI CGRYCG 1 cut(s) 655
BsiHKAI GWGCWC 1 cut(s) 276
BsiSI CCGG 6 cut(s) 20, 224, 302, 407, 422, 647
BslFI GGGAC 3 cut(s) 112, 152, 755
BslI CCNNNNNNNGG 2 cut(s) 301, 457
BsmAI GTCTC 1 cut(s) 428
BsmBI CGTCTC 1 cut(s) 428
BsmFI GGGAC 3 cut(s) 112, 152, 755
BsnI GGCC 4 cut(s) 320, 352, 583, 625
Bsp119I TTCGAA 1 cut(s) 211
Bsp120I GGGCCC 2 cut(s) 581, 623
Bsp1286I GDGCHC 3 cut(s) 276, 585, 627
Bsp143I GATC 4 cut(s) 58, 285, 591, 652
Bsp1720I GCTNAGC 1 cut(s) 446
Bsp68I TCGCGA 1 cut(s) 242
BspANI GGCC 4 cut(s) 320, 352, 583, 625
BspCNI CTCAG 2 cut(s) 383, 438
BspFNI CGCG 5 cut(s) 242, 633, 816, 818, 851
BspLI GGNNCC 4 cut(s) 24, 582, 583, 625
BspPI GGATC 2 cut(s) 53, 280
BspT104I TTCGAA 1 cut(s) 211
BspT107I GGYRCC 1 cut(s) 22
BsrBI CCGCTC 1 cut(s) 867
BsrFI RCCGGY 1 cut(s) 301
BsrI ACTGG 1 cut(s) 54
BssAI RCCGGY 1 cut(s) 301
BssECI CCNNGG 2 cut(s) 321, 422
BssMI GATC 4 cut(s) 58, 285, 591, 652
BssNI GRCGYC 4 cut(s) 23, 386, 392, 398
Bst2UI CCWGG 2 cut(s) 323, 452
Bst4CI ACNGT 3 cut(s) 220, 363, 680
Bst6I CTCTTC 2 cut(s) 693, 815
BstACI GRCGYC 4 cut(s) 23, 386, 392, 398
BstBAI YACGTR 1 cut(s) 571
BstBI TTCGAA 1 cut(s) 211
BstC8I GCNNGC 3 cut(s) 191, 384, 519
BstDEI CTNAG 2 cut(s) 370, 446
BstF5I GGATG 2 cut(s) 61, 831
BstFNI CGCG 5 cut(s) 242, 633, 816, 818, 851
BstH2I RGCGCY 2 cut(s) 26, 332
BstHHI GCGC 6 cut(s) 7, 25, 331, 523, 818, 853
BstKTI GATC 4 cut(s) 61, 288, 594, 655
BstMAI GTCTC 1 cut(s) 428
BstMBI GATC 4 cut(s) 58, 285, 591, 652
BstMCI CGRYCG 1 cut(s) 655
BstMWI GCNNNNNNNGC 4 cut(s) 186, 271, 323, 326
BstNI CCWGG 2 cut(s) 323, 452
BstSCI CCNGG 3 cut(s) 321, 421, 450
BstSLI GKGCMC 2 cut(s) 585, 627
BstUI CGCG 5 cut(s) 242, 633, 816, 818, 851
BstV1I GCAGC 1 cut(s) 338
BstV2I GAAGAC 2 cut(s) 44, 125
BstX2I RGATCY 1 cut(s) 285
BstYI RGATCY 1 cut(s) 285
BsuRI GGCC 4 cut(s) 320, 352, 583, 625
BtgZI GCGATG 1 cut(s) 648
BtrI CACGTC 1 cut(s) 76
BtsCI GGATG 2 cut(s) 61, 831
BtsI GCAGTG 1 cut(s) 542
BtsIMutI CAGTG 1 cut(s) 542
BtuMI TCGCGA 1 cut(s) 242
Cac8I GCNNGC 3 cut(s) 191, 384, 519
CfoI GCGC 6 cut(s) 7, 25, 331, 523, 818, 853
Cfr10I RCCGGY 1 cut(s) 301
Cfr13I GGNCC 7 cut(s) 12, 319, 350, 581, 582, 623, 624
CseI GACGC 2 cut(s) 375, 451
Csp6I GTAC 1 cut(s) 568
CspAI ACCGGT 1 cut(s) 301
CviAII CATG 1 cut(s) 84
CviQI GTAC 1 cut(s) 568
DdeI CTNAG 2 cut(s) 370, 446
DinI GGCGCC 1 cut(s) 24
DpnI GATC 4 cut(s) 60, 287, 593, 654
DpnII GATC 4 cut(s) 58, 285, 591, 652
DrdI GACNNNNNNGTC 1 cut(s) 397
DseDI GACNNNNNNGTC 1 cut(s) 397
Eam1104I CTCTTC 2 cut(s) 693, 815
EarI CTCTTC 2 cut(s) 693, 815
EciI GGCGGA 3 cut(s) 176, 278, 473
Ecl136II GAGCTC 1 cut(s) 274
Eco24I GRGCYC 3 cut(s) 276, 585, 627
Eco32I GATATC 1 cut(s) 81
Eco47I GGWCC 1 cut(s) 12
Eco47III AGCGCT 1 cut(s) 330
Eco53kI GAGCTC 1 cut(s) 274
Eco57I CTGAAG 2 cut(s) 506, 734
EcoICRI GAGCTC 1 cut(s) 274
EcoO109I RGGNCCY 1 cut(s) 581
EcoRII CCWGG 2 cut(s) 321, 450
EcoRV GATATC 1 cut(s) 81
EcoT38I GRGCYC 3 cut(s) 276, 585, 627
EgeI GGCGCC 1 cut(s) 24
EheI GGCGCC 1 cut(s) 24
Esp3I CGTCTC 1 cut(s) 428
FaeI CATG 1 cut(s) 87
FaiI YATR 4 cut(s) 85, 204, 621, 674
FaqI GGGAC 3 cut(s) 112, 152, 755
FatI CATG 1 cut(s) 83
FauI CCCGC 4 cut(s) 483, 638, 821, 860
FblI GTMKAC 2 cut(s) 389, 395
Fnu4HI GCNGC 4 cut(s) 292, 318, 327, 835
FokI GGATG 2 cut(s) 68, 838
FriOI GRGCYC 3 cut(s) 276, 585, 627
Fsp4HI GCNGC 4 cut(s) 292, 318, 327, 835
FspBI CTAG 2 cut(s) 332, 883
GlaI GCGC 6 cut(s) 6, 24, 330, 522, 817, 852
GluI GCNGC 4 cut(s) 292, 318, 327, 835
GsuI CTGGAG 1 cut(s) 366
HaeII RGCGCY 2 cut(s) 26, 332
HaeIII GGCC 4 cut(s) 320, 352, 583, 625
HapII CCGG 6 cut(s) 20, 224, 302, 407, 422, 647
HgaI GACGC 2 cut(s) 375, 451
HhaI GCGC 6 cut(s) 7, 25, 331, 523, 818, 853
Hin1I GRCGYC 4 cut(s) 23, 386, 392, 398
Hin1II CATG 1 cut(s) 87
Hin6I GCGC 6 cut(s) 5, 23, 329, 521, 816, 851
HinP1I GCGC 6 cut(s) 5, 23, 329, 521, 816, 851
HincII GTYRAC 2 cut(s) 390, 396
HindII GTYRAC 2 cut(s) 390, 396
HindIII AAGCTT 1 cut(s) 178
HinfI GANTC 4 cut(s) 121, 410, 418, 502
HpaII CCGG 6 cut(s) 20, 224, 302, 407, 422, 647
HphI GGTGA 4 cut(s) 316, 347, 695, 745
Hpy166II GTNNAC 2 cut(s) 390, 396
Hpy188I TCNGA 6 cut(s) 33, 63, 283, 469, 507, 514
Hpy188III TCNNGA 4 cut(s) 125, 241, 345, 668
Hpy8I GTNNAC 2 cut(s) 390, 396
HpyAV CCTTC 7 cut(s) 262, 697, 701, 730, 748, 823, 865
HpyCH4III ACNGT 3 cut(s) 220, 363, 680
HpyCH4IV ACGT 6 cut(s) 41, 75, 104, 392, 398, 570
HpyCH4V TGCA 1 cut(s) 134
HpyF10VI GCNNNNNNNGC 4 cut(s) 186, 271, 323, 326
HpyF3I CTNAG 2 cut(s) 370, 446
HpySE526I ACGT 6 cut(s) 41, 75, 104, 392, 398, 570
Hsp92I GRCGYC 4 cut(s) 23, 386, 392, 398
Hsp92II CATG 1 cut(s) 87
HspAI GCGC 6 cut(s) 5, 23, 329, 521, 816, 851
KasI GGCGCC 1 cut(s) 22
Kzo9I GATC 4 cut(s) 58, 285, 591, 652
LmnI GCTCC 5 cut(s) 271, 279, 379, 514, 864
Lsp1109I GCAGC 1 cut(s) 338
LweI GCATC 1 cut(s) 609
MaeI CTAG 2 cut(s) 332, 883
MaeII ACGT 6 cut(s) 41, 75, 104, 392, 398, 570
MaeIII GTNAC 2 cut(s) 304, 878
MalI GATC 4 cut(s) 60, 287, 593, 654
MbiI CCGCTC 1 cut(s) 867
MboI GATC 4 cut(s) 58, 285, 591, 652
MboII GAAGA 8 cut(s) 49, 130, 427, 586, 707, 710, 772, 832
MflI RGATCY 1 cut(s) 285
MhlI GDGCHC 3 cut(s) 276, 585, 627
MluCI AATT 3 cut(s) 169, 250, 782
Mly113I GGCGCC 1 cut(s) 23
MlyI GAGTC 2 cut(s) 115, 404
MnlI CCTC 5 cut(s) 254, 341, 370, 816, 855
MspI CCGG 6 cut(s) 20, 224, 302, 407, 422, 647
MspR9I CCNGG 3 cut(s) 323, 423, 452
MvaI CCWGG 2 cut(s) 323, 452
MvnI CGCG 5 cut(s) 242, 633, 816, 818, 851
MwoI GCNNNNNNNGC 4 cut(s) 186, 271, 323, 326
NarI GGCGCC 1 cut(s) 23
NciI CCSGG 1 cut(s) 423
NdeII GATC 4 cut(s) 58, 285, 591, 652
NlaIII CATG 1 cut(s) 87
NlaIV GGNNCC 4 cut(s) 24, 582, 583, 625
NmuCI GTSAC 1 cut(s) 304
NruI TCGCGA 1 cut(s) 242
NspV TTCGAA 1 cut(s) 211
PcsI WCGNNNNNNNCGW 3 cut(s) 362, 395, 398
PfeI GAWTC 2 cut(s) 418, 502
PflFI GACNNNGTC 1 cut(s) 361
PinAI ACCGGT 1 cut(s) 301
PkrI GCNGC 4 cut(s) 293, 319, 328, 836
Ple19I CGATCG 1 cut(s) 655
PleI GAGTC 2 cut(s) 115, 404
PluTI GGCGCC 1 cut(s) 26
PpsI GAGTC 2 cut(s) 115, 404
Ppu21I YACGTR 1 cut(s) 571
Psp124BI GAGCTC 1 cut(s) 276
Psp6I CCWGG 2 cut(s) 321, 450
PspGI CCWGG 2 cut(s) 321, 450
PspN4I GGNNCC 4 cut(s) 24, 582, 583, 625
PspOMI GGGCCC 2 cut(s) 581, 623
PspPI GGNCC 7 cut(s) 12, 319, 350, 581, 582, 623, 624
PsuI RGATCY 1 cut(s) 285
PsyI GACNNNGTC 1 cut(s) 361
PvuI CGATCG 1 cut(s) 655
RruI TCGCGA 1 cut(s) 242
RsaI GTAC 1 cut(s) 569
RsaNI GTAC 1 cut(s) 568
SacI GAGCTC 1 cut(s) 276
SalI GTCGAC 2 cut(s) 388, 394
SatI GCNGC 4 cut(s) 292, 318, 327, 835
Sau3AI GATC 4 cut(s) 58, 285, 591, 652
Sau96I GGNCC 7 cut(s) 12, 319, 350, 581, 582, 623, 624
SchI GAGTC 2 cut(s) 115, 404
ScrFI CCNGG 3 cut(s) 323, 423, 452
SduI GDGCHC 3 cut(s) 276, 585, 627
SfaNI GCATC 1 cut(s) 609
SfoI GGCGCC 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 211
SgrDI CGTCGACG 2 cut(s) 388, 394
SinI GGWCC 1 cut(s) 12
SmlI CTYRAG 1 cut(s) 175
SmoI CTYRAG 1 cut(s) 175
Sse9I AATT 3 cut(s) 169, 250, 782
SspDI GGCGCC 1 cut(s) 22
SspI AATATT 1 cut(s) 155
SspMI CTAG 2 cut(s) 332, 883
SstI GAGCTC 1 cut(s) 276
StyD4I CCNGG 3 cut(s) 321, 421, 450
TaaI ACNGT 3 cut(s) 220, 363, 680
TaiI ACGT 6 cut(s) 44, 78, 107, 395, 401, 573
TaqI TCGA 6 cut(s) 211, 356, 365, 389, 395, 413
TasI AATT 3 cut(s) 169, 250, 782
TauI GCSGC 3 cut(s) 294, 320, 837
TfiI GAWTC 2 cut(s) 418, 502
TscAI CASTG 1 cut(s) 542
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 1 cut(s) 326
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 3 cut(s) 105, 767, 842
TspGWI ACGGA 1 cut(s) 4
TspRI CASTG 1 cut(s) 542
Tth111I GACNNNGTC 1 cut(s) 361
VpaK11BI GGWCC 1 cut(s) 12
XapI RAATTY 2 cut(s) 250, 782
XmiI GTMKAC 2 cut(s) 389, 395
XspI CTAG 2 cut(s) 332, 883
ZraI GACGTC 2 cut(s) 393, 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.