RLG00000017444

heat shock factor protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013192
Physical Location & Seq
Reverse (-)
16843778 .. 16847739
3962 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017444

Sequence Viewer

Length: 888 bp
ATGATCAAAATGGCTCAAAGGTCAGTTCCGGCGCCGTTTCTGATGAAGACGTTCCAGTTGGTGGATGATCCGGCCAGCGACGACGTGATATCTTGGAACGGAAGTGGGACGGCTTTTGTGGTTTGGAAGACGGTGGATTTCGCCCGGGATATGTTGCCTAATTATTTCAAGCACAACAACTTCTCAAGCTTCGTTCGCCAGCTTAACACCTATGGCTTTCGAAAGACCGTGCCGGACAAATGGGAGTTTGCGAACGACAACTTCCGGCGAGGTGAGAAGGAGCTGCTCTCCGAGATCCGCCGTCGCAAATCGGTGTCTTCATCTCCGGCTCAGGCCAACGCCGAGGAAAAATCCGGCGGAGACGCCCCGTCAACCCCGTCCAACTCCGGCGAGGACCTGGCGTCAACTTCCACGTCTTCGCCGGACTCGAAGAATCCGGGATCGGTGGAGACAGCGGCGGCGGTGTGCCAGGCGGTGGACTTATCCGGCGAGAACGAGAAACTGAAGAAAGACAACGAGACGCTGAGCTCGGAGCTTGCACAAACAAAGAAGCAGTGTGATGAGCTGGTGGCGTTTCTGATGGAGTACATGAAGGTCGGGCCCGATCAGATCAATCAGATTATGCGGCAAGGAAGCTTAGGGTCCAGCTGTGATGTTGATGATGGTGGTCGTGGTCACAGTGAGAATGATAATGACGAAAAAGTTGTGGCGGGAGAAGAAGAGGGTTTGAAACTGTTTGGGGTTTGGCTGAAGGGGAACGAGAACGAGGAGAAGAAGAAAAGGCGGGAGGAGAAATCGGGTGGTGGTGGTGGTGGGCCGCAATCGAAGAAGATGAAGATGGCGGAGTTTCACGCGCCGCTGAGGAAGAGCGGGAAGGTGTGCAACTGA

Protein Analysis

296

Amino Acids

32.4

Weight (kDa)

5.83

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 13 - 102 1.3e-29 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013192)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36990
fragaria_vesca FvH4_1g16030
malus_domestica MD02G1171800.v1.1 MD15G1283700.v1.1
prunus_persica Prupe.7G133600_v2.0.a1
pyrus_communis pycom02g13850 pycom15g24670
rosa_chinensis RchiOBHm_Chr2g0105601
rosa_laevigata RLG00000017444
rosa_multiflora Rmu_co8474487.1_g000001 Rmu_sc0001051.1_g000029
rosa_roxburghii Rroxscaffold_2G00138040
rosa_rugosa Rorug02G0129000
rosa_samantha Rh2AG180500 Rh2BG189300 Rh2CG184700 Rh2DG186200
rosa_wichuraiana Rw2G014110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 31
AccB7I CCANNNNNTGG 2 cut(s) 61, 475
AccBSI CCGCTC 1 cut(s) 870
AccII CGCG 1 cut(s) 854
AclWI GGATC 3 cut(s) 62, 289, 448
AcoI YGGCCR 1 cut(s) 72
AcuI CTGAAG 2 cut(s) 524, 770
AcyI GRCGYC 3 cut(s) 32, 363, 401
AfaI GTAC 1 cut(s) 587
AfiI CCNNNNNNNGG 2 cut(s) 61, 475
AgsI TTSAA 2 cut(s) 169, 730
AhdI GACNNNNNGTC 2 cut(s) 367, 400
AjiI CACGTC 2 cut(s) 85, 414
AjnI CCWGG 2 cut(s) 396, 468
AluBI AGCT 8 cut(s) 189, 202, 283, 528, 535, 565, 636, 648
AluI AGCT 8 cut(s) 189, 202, 283, 528, 535, 565, 636, 648
Alw21I GWGCWC 1 cut(s) 530
Alw26I GTCTC 3 cut(s) 354, 443, 512
AlwI GGATC 3 cut(s) 62, 289, 448
Ama87I CYCGRG 1 cut(s) 144
AoxI GGCC 4 cut(s) 72, 333, 599, 815
ApaI GGGCCC 1 cut(s) 603
ApeKI GCWGC 1 cut(s) 283
Asp700I GAANNNNTTC 1 cut(s) 50
AspLEI GCGC 2 cut(s) 34, 856
AspS9I GGNCC 5 cut(s) 394, 599, 600, 642, 815
AsuC2I CCSGG 3 cut(s) 145, 146, 438
AsuHPI GGTGA 1 cut(s) 284
AsuII TTCGAA 1 cut(s) 220
AvaI CYCGRG 1 cut(s) 144
AvaII GGWCC 2 cut(s) 394, 642
BaeGI GKGCMC 1 cut(s) 603
BanI GGYRCC 1 cut(s) 31
BanII GRGCYC 2 cut(s) 530, 603
BbsI GAAGAC 4 cut(s) 53, 134, 309, 408
Bbv12I GWGCWC 1 cut(s) 530
BbvCI CCTCAGC 1 cut(s) 860
BbvI GCAGC 1 cut(s) 270
BccI CCATC 3 cut(s) 574, 656, 832
BceAI ACGGC 3 cut(s) 19, 126, 285
BciT130I CCWGG 2 cut(s) 398, 470
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 3 cut(s) 145, 146, 438
BcoDI GTCTC 3 cut(s) 354, 443, 512
BfoI RGCGCY 1 cut(s) 35
BisI GCNGC 6 cut(s) 284, 456, 459, 626, 818, 857
BlpI GCTNAGC 1 cut(s) 524
BlsI GCNGC 6 cut(s) 285, 457, 460, 627, 819, 858
Bme1390I CCNGG 5 cut(s) 145, 146, 398, 438, 470
Bme18I GGWCC 2 cut(s) 394, 642
BmeRI GACNNNNNGTC 2 cut(s) 367, 400
BmeT110I CYCGRG 1 cut(s) 144
BmgBI CACGTC 2 cut(s) 85, 414
BmgT120I GGNCC 5 cut(s) 394, 599, 600, 642, 815
BmiI GGNNCC 3 cut(s) 33, 601, 643
BmrFI CCNGG 5 cut(s) 145, 146, 398, 438, 470
BpiI GAAGAC 4 cut(s) 53, 134, 309, 408
BplI GAGNNNNNCTC 2 cut(s) 272, 304
Bpu10I CCTNAGC 3 cut(s) 330, 637, 860
Bpu1102I GCTNAGC 1 cut(s) 524
Bpu14I TTCGAA 1 cut(s) 220
BpuEI CTTGAG 1 cut(s) 169
BpuMI CCSGG 3 cut(s) 145, 146, 438
BsaHI GRCGYC 3 cut(s) 32, 363, 401
BsaJI CCNNGG 2 cut(s) 144, 342
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 475
Bse1I ACTGG 1 cut(s) 55
BseBI CCWGG 2 cut(s) 398, 470
BseDI CCNNGG 2 cut(s) 144, 342
BseGI GGATG 1 cut(s) 70
BseLI CCNNNNNNNGG 2 cut(s) 61, 475
BseMII CTCAG 3 cut(s) 344, 515, 851
BseNI ACTGG 1 cut(s) 55
BseRI GAGGAG 2 cut(s) 782, 803
BseSI GKGCMC 1 cut(s) 603
BseXI GCAGC 1 cut(s) 270
Bsh1236I CGCG 1 cut(s) 854
BshFI GGCC 4 cut(s) 74, 335, 601, 817
BshNI GGYRCC 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 530
BsiHKCI CYCGRG 1 cut(s) 144
BslFI GGGAC 1 cut(s) 121
BslI CCNNNNNNNGG 2 cut(s) 61, 475
BsmAI GTCTC 3 cut(s) 354, 443, 512
BsmBI CGTCTC 2 cut(s) 354, 512
BsmFI GGGAC 1 cut(s) 121
BsnI GGCC 4 cut(s) 74, 335, 601, 817
BsoBI CYCGRG 1 cut(s) 144
Bsp119I TTCGAA 1 cut(s) 220
Bsp120I GGGCCC 1 cut(s) 599
Bsp1286I GDGCHC 2 cut(s) 530, 603
Bsp143I GATC 6 cut(s) 3, 67, 294, 440, 604, 609
Bsp1720I GCTNAGC 1 cut(s) 524
BspANI GGCC 4 cut(s) 74, 335, 601, 817
BspCNI CTCAG 3 cut(s) 343, 516, 852
BspFNI CGCG 1 cut(s) 854
BspLI GGNNCC 3 cut(s) 33, 601, 643
BspPI GGATC 3 cut(s) 62, 289, 448
BspQI GCTCTTC 1 cut(s) 860
BspT104I TTCGAA 1 cut(s) 220
BspT107I GGYRCC 1 cut(s) 31
BsrBI CCGCTC 1 cut(s) 870
BsrI ACTGG 1 cut(s) 55
BssECI CCNNGG 2 cut(s) 144, 342
BssMI GATC 6 cut(s) 3, 67, 294, 440, 604, 609
BssNI GRCGYC 3 cut(s) 32, 363, 401
Bst2UI CCWGG 2 cut(s) 398, 470
Bst4CI ACNGT 4 cut(s) 133, 229, 680, 735
Bst6I CTCTTC 2 cut(s) 714, 860
BstACI GRCGYC 3 cut(s) 32, 363, 401
BstBI TTCGAA 1 cut(s) 220
BstC8I GCNNGC 3 cut(s) 76, 200, 537
BstDEI CTNAG 4 cut(s) 330, 524, 637, 860
BstF5I GGATG 1 cut(s) 70
BstFNI CGCG 1 cut(s) 854
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 2 cut(s) 34, 856
BstKTI GATC 6 cut(s) 6, 70, 297, 443, 607, 612
BstMAI GTCTC 3 cut(s) 354, 443, 512
BstMBI GATC 6 cut(s) 3, 67, 294, 440, 604, 609
BstMWI GCNNNNNNNGC 1 cut(s) 195
BstNI CCWGG 2 cut(s) 398, 470
BstSCI CCNGG 5 cut(s) 143, 144, 396, 436, 468
BstSLI GKGCMC 1 cut(s) 603
BstUI CGCG 1 cut(s) 854
BstV1I GCAGC 1 cut(s) 270
BstV2I GAAGAC 4 cut(s) 53, 134, 309, 408
BstX2I RGATCY 1 cut(s) 294
BstYI RGATCY 1 cut(s) 294
BsuRI GGCC 4 cut(s) 74, 335, 601, 817
BtrI CACGTC 2 cut(s) 85, 414
BtsCI GGATG 1 cut(s) 70
BtsI GCAGTG 1 cut(s) 560
BtsIMutI CAGTG 2 cut(s) 560, 685
Cac8I GCNNGC 3 cut(s) 76, 200, 537
CfoI GCGC 2 cut(s) 34, 856
Cfr13I GGNCC 5 cut(s) 394, 599, 600, 642, 815
Cfr9I CCCGGG 1 cut(s) 144
CseI GACGC 3 cut(s) 371, 390, 529
Csp6I GTAC 1 cut(s) 586
CviAII CATG 1 cut(s) 589
CviQI GTAC 1 cut(s) 586
DdeI CTNAG 4 cut(s) 330, 524, 637, 860
DinI GGCGCC 1 cut(s) 33
DpnI GATC 6 cut(s) 5, 69, 296, 442, 606, 611
DpnII GATC 6 cut(s) 3, 67, 294, 440, 604, 609
DriI GACNNNNNGTC 2 cut(s) 367, 400
EaeI YGGCCR 1 cut(s) 72
Eam1104I CTCTTC 2 cut(s) 714, 860
Eam1105I GACNNNNNGTC 2 cut(s) 367, 400
EarI CTCTTC 2 cut(s) 714, 860
EciI GGCGGA 3 cut(s) 287, 372, 857
Ecl136II GAGCTC 1 cut(s) 528
Eco24I GRGCYC 2 cut(s) 530, 603
Eco32I GATATC 1 cut(s) 90
Eco47I GGWCC 2 cut(s) 394, 642
Eco53kI GAGCTC 1 cut(s) 528
Eco57I CTGAAG 2 cut(s) 524, 770
Eco88I CYCGRG 1 cut(s) 144
EcoICRI GAGCTC 1 cut(s) 528
EcoO109I RGGNCCY 1 cut(s) 394
EcoRII CCWGG 2 cut(s) 396, 468
EcoRV GATATC 1 cut(s) 90
EcoT38I GRGCYC 2 cut(s) 530, 603
EgeI GGCGCC 1 cut(s) 33
EheI GGCGCC 1 cut(s) 33
Esp3I CGTCTC 2 cut(s) 354, 512
FaeI CATG 1 cut(s) 592
FaiI YATR 4 cut(s) 152, 213, 590, 623
FaqI GGGAC 1 cut(s) 121
FatI CATG 1 cut(s) 588
FauI CCCGC 3 cut(s) 703, 777, 863
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 6 cut(s) 284, 456, 459, 626, 818, 857
FokI GGATG 1 cut(s) 77
FriOI GRGCYC 2 cut(s) 530, 603
Fsp4HI GCNGC 6 cut(s) 284, 456, 459, 626, 818, 857
GlaI GCGC 2 cut(s) 33, 855
GluI GCNGC 6 cut(s) 284, 456, 459, 626, 818, 857
HaeII RGCGCY 1 cut(s) 35
HaeIII GGCC 4 cut(s) 74, 335, 601, 817
HgaI GACGC 3 cut(s) 371, 390, 529
HhaI GCGC 2 cut(s) 34, 856
Hin1I GRCGYC 3 cut(s) 32, 363, 401
Hin1II CATG 1 cut(s) 592
Hin6I GCGC 2 cut(s) 32, 854
HinP1I GCGC 2 cut(s) 32, 854
HincII GTYRAC 2 cut(s) 372, 405
HindII GTYRAC 2 cut(s) 372, 405
HindIII AAGCTT 2 cut(s) 187, 634
HinfI GANTC 2 cut(s) 425, 433
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 3 cut(s) 372, 405, 478
Hpy188I TCNGA 6 cut(s) 42, 292, 532, 579, 609, 618
Hpy8I GTNNAC 3 cut(s) 372, 405, 478
Hpy99I CGWCG 3 cut(s) 83, 86, 306
HpyAV CCTTC 4 cut(s) 271, 586, 745, 868
HpyCH4III ACNGT 4 cut(s) 133, 229, 680, 735
HpyCH4IV ACGT 3 cut(s) 50, 84, 413
HpyCH4V TGCA 2 cut(s) 539, 882
HpyF10VI GCNNNNNNNGC 1 cut(s) 195
HpyF3I CTNAG 4 cut(s) 330, 524, 637, 860
HpySE526I ACGT 3 cut(s) 50, 84, 413
Hsp92I GRCGYC 3 cut(s) 32, 363, 401
Hsp92II CATG 1 cut(s) 592
HspAI GCGC 2 cut(s) 32, 854
KasI GGCGCC 1 cut(s) 31
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 6 cut(s) 3, 67, 294, 440, 604, 609
LguI GCTCTTC 1 cut(s) 860
LmnI GCTCC 2 cut(s) 280, 532
Lsp1109I GCAGC 1 cut(s) 270
MaeII ACGT 3 cut(s) 50, 84, 413
MaeIII GTNAC 1 cut(s) 674
MalI GATC 6 cut(s) 5, 69, 296, 442, 606, 611
MbiI CCGCTC 1 cut(s) 870
MboI GATC 6 cut(s) 3, 67, 294, 440, 604, 609
MflI RGATCY 1 cut(s) 294
MhlI GDGCHC 2 cut(s) 530, 603
MluCI AATT 1 cut(s) 160
Mly113I GGCGCC 1 cut(s) 32
MlyI GAGTC 1 cut(s) 419
MmeI TCCRAC 1 cut(s) 405
MnlI CCTC 7 cut(s) 263, 337, 385, 715, 760, 781, 855
MroXI GAANNNNTTC 1 cut(s) 50
MseI TTAA 1 cut(s) 204
MspA1I CMGCKG 3 cut(s) 455, 648, 859
MspR9I CCNGG 5 cut(s) 145, 146, 398, 438, 470
MvaI CCWGG 2 cut(s) 398, 470
MvnI CGCG 1 cut(s) 854
MwoI GCNNNNNNNGC 1 cut(s) 195
NarI GGCGCC 1 cut(s) 32
NciI CCSGG 3 cut(s) 145, 146, 438
NdeII GATC 6 cut(s) 3, 67, 294, 440, 604, 609
NlaIII CATG 1 cut(s) 592
NlaIV GGNNCC 3 cut(s) 33, 601, 643
NmeAIII GCCGAG 1 cut(s) 367
NmuCI GTSAC 1 cut(s) 674
NspV TTCGAA 1 cut(s) 220
PciSI GCTCTTC 1 cut(s) 860
PcsI WCGNNNNNNNCGW 1 cut(s) 425
PdmI GAANNNNTTC 1 cut(s) 50
PfeI GAWTC 1 cut(s) 433
PflMI CCANNNNNTGG 2 cut(s) 61, 475
PfoI TCCNGGA 1 cut(s) 436
PkrI GCNGC 6 cut(s) 285, 457, 460, 627, 819, 858
PleI GAGTC 1 cut(s) 419
PluTI GGCGCC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 419
PpuMI RGGWCCY 1 cut(s) 394
Psp124BI GAGCTC 1 cut(s) 530
Psp5II RGGWCCY 1 cut(s) 394
Psp6I CCWGG 2 cut(s) 396, 468
PspGI CCWGG 2 cut(s) 396, 468
PspN4I GGNNCC 3 cut(s) 33, 601, 643
PspOMI GGGCCC 1 cut(s) 599
PspPI GGNCC 5 cut(s) 394, 599, 600, 642, 815
PspPPI RGGWCCY 1 cut(s) 394
PsuI RGATCY 1 cut(s) 294
PvuII CAGCTG 1 cut(s) 648
RsaI GTAC 1 cut(s) 587
RsaNI GTAC 1 cut(s) 586
SacI GAGCTC 1 cut(s) 530
SapI GCTCTTC 1 cut(s) 860
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 6 cut(s) 284, 456, 459, 626, 818, 857
Sau3AI GATC 6 cut(s) 3, 67, 294, 440, 604, 609
Sau96I GGNCC 5 cut(s) 394, 599, 600, 642, 815
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 5 cut(s) 145, 146, 398, 438, 470
SduI GDGCHC 2 cut(s) 530, 603
SfoI GGCGCC 1 cut(s) 33
SfuI TTCGAA 1 cut(s) 220
SinI GGWCC 2 cut(s) 394, 642
SmaI CCCGGG 1 cut(s) 146
SmlI CTYRAG 1 cut(s) 184
SmoI CTYRAG 1 cut(s) 184
Sse9I AATT 1 cut(s) 160
SspDI GGCGCC 1 cut(s) 31
SstI GAGCTC 1 cut(s) 530
StyD4I CCNGG 5 cut(s) 143, 144, 396, 436, 468
TaaI ACNGT 4 cut(s) 133, 229, 680, 735
TaiI ACGT 3 cut(s) 53, 87, 416
TaqI TCGA 3 cut(s) 220, 428, 824
TasI AATT 1 cut(s) 160
TatI WGTACW 1 cut(s) 585
TauI GCSGC 5 cut(s) 458, 461, 628, 820, 859
TfiI GAWTC 1 cut(s) 433
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TscAI CASTG 2 cut(s) 560, 685
TseFI GTSAC 1 cut(s) 674
TseI GCWGC 1 cut(s) 283
Tsp45I GTSAC 1 cut(s) 674
TspDTI ATGAA 4 cut(s) 59, 309, 605, 848
TspGWI ACGGA 1 cut(s) 114
TspMI CCCGGG 1 cut(s) 144
TspRI CASTG 2 cut(s) 560, 685
Van91I CCANNNNNTGG 2 cut(s) 61, 475
VpaK11BI GGWCC 2 cut(s) 394, 642
XmaI CCCGGG 1 cut(s) 144
XmnI GAANNNNTTC 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.