Rw2G014110

heat shock factor protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013192
Physical Location & Seq
Reverse (-)
15625280 .. 15627967
2688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G014110.1

Sequence Viewer

Length: 891 bp
ATGCAGATGATCAAAATGGCTCAAAGGTCAGTTCCGGCGCCGTTTCTGATGAAGACGTTCCAGTTGGTGGATGATCCGGCCAGCGACGACGTGATATCTTGGAACGGAAATGGGACAGCGTTTGTGGTTTGGAAGACGGTGGATTTCGCCCGGGATATGTTGCCTAATTATTTCAAGCACAACAACTTCTCAAGCTTCGTTCGCCAGCTTAACACCTATGGCTTTCGAAAGACCGTGCCGGACAAATGGGAGTTCGCGAACGACAACTTCCGGCGAGGTGAGAAGGAGCTGCTCTCCGAGATCCGTCGCCGAAAATCTGTGTCGTCATCTCAGGCTCAGGCAAACGCAGCGGAAAAATCCGGCGGAGACGCCCCGTCAACCCCGTCCAACTCCGGCGAGGACCTGGCGTCAACTTCCACGTCTTCGCCGGACTCGAAGAATCCGGGATCGGTGGAGACAGCGGCGGCGGTGTGCCAGGCGGTGGACTTATCCGGCGAGAACGAGAAACTGAAGAAAGACAACGAGACGCTGAGCTCGGAGCTTGCACAAACAAAGAAGCAGTGTGATGAGCTGGTGGCGTTTCTGATGGAGTACATGAAGGTCGGGCCCGATCAGATCAATCAGATTATGCGGCAAGGAAGCTTAGGGTCCAGCTGTGATGTTGATGATCTTGGTCGTGGTCACACTGAGAATGATAATGACGAAAAAGTTGTGGCGGGAGGAGAAGAGGGTTTGAAACTGTTTGGGGTTTGGCTGAAGGGGAACGAGAACGAGGAGAAGAAGAAAAGGAGGGAGAAGAAATCGGGTGGTGGTGGTGGGCCGCAAGCGAAGAAGATGAAGATGGCGGAGTTTCACGCGCCGCTGAGGAAGAGCGGGAAGGTGTGCAACTAA

Protein Analysis

296

Amino Acids

32.59

Weight (kDa)

7.61

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 15 - 104 8.7e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013192)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36990
fragaria_vesca FvH4_1g16030
malus_domestica MD02G1171800.v1.1 MD15G1283700.v1.1
prunus_persica Prupe.7G133600_v2.0.a1
pyrus_communis pycom02g13850 pycom15g24670
rosa_chinensis RchiOBHm_Chr2g0105601
rosa_laevigata RLG00000017444
rosa_multiflora Rmu_co8474487.1_g000001 Rmu_sc0001051.1_g000029
rosa_roxburghii Rroxscaffold_2G00138040
rosa_rugosa Rorug02G0129000
rosa_samantha Rh2AG180500 Rh2BG189300 Rh2CG184700 Rh2DG186200
rosa_wichuraiana Rw2G014110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 37
AccB7I CCANNNNNTGG 2 cut(s) 67, 481
AccBSI CCGCTC 1 cut(s) 873
AccII CGCG 2 cut(s) 257, 857
AclWI GGATC 3 cut(s) 68, 295, 454
AcoI YGGCCR 1 cut(s) 78
AcuI CTGAAG 2 cut(s) 530, 776
AcyI GRCGYC 3 cut(s) 38, 369, 407
AfaI GTAC 1 cut(s) 593
AfiI CCNNNNNNNGG 2 cut(s) 67, 481
AgsI TTSAA 2 cut(s) 175, 736
AhdI GACNNNNNGTC 2 cut(s) 373, 406
AjiI CACGTC 2 cut(s) 91, 420
AjnI CCWGG 2 cut(s) 402, 474
AluBI AGCT 8 cut(s) 195, 208, 289, 534, 541, 571, 642, 654
AluI AGCT 8 cut(s) 195, 208, 289, 534, 541, 571, 642, 654
Alw21I GWGCWC 1 cut(s) 536
Alw26I GTCTC 3 cut(s) 360, 449, 518
AlwI GGATC 3 cut(s) 68, 295, 454
Ama87I CYCGRG 1 cut(s) 150
AoxI GGCC 3 cut(s) 78, 605, 818
ApaI GGGCCC 1 cut(s) 609
ApeKI GCWGC 2 cut(s) 289, 347
Asp700I GAANNNNTTC 1 cut(s) 56
AspLEI GCGC 2 cut(s) 40, 859
AspS9I GGNCC 5 cut(s) 400, 605, 606, 648, 818
AsuC2I CCSGG 3 cut(s) 151, 152, 444
AsuHPI GGTGA 1 cut(s) 290
AsuII TTCGAA 1 cut(s) 226
AvaI CYCGRG 1 cut(s) 150
AvaII GGWCC 2 cut(s) 400, 648
BaeGI GKGCMC 1 cut(s) 609
BanI GGYRCC 1 cut(s) 37
BanII GRGCYC 2 cut(s) 536, 609
BbsI GAAGAC 3 cut(s) 59, 140, 414
Bbv12I GWGCWC 1 cut(s) 536
BbvCI CCTCAGC 1 cut(s) 863
BbvI GCAGC 2 cut(s) 276, 359
BccI CCATC 2 cut(s) 580, 835
BceAI ACGGC 1 cut(s) 25
BciT130I CCWGG 2 cut(s) 404, 476
BclI TGATCA 1 cut(s) 9
BcnI CCSGG 3 cut(s) 151, 152, 444
BcoDI GTCTC 3 cut(s) 360, 449, 518
BfoI RGCGCY 1 cut(s) 41
BisI GCNGC 7 cut(s) 290, 348, 462, 465, 632, 821, 860
BlpI GCTNAGC 1 cut(s) 530
BlsI GCNGC 7 cut(s) 291, 349, 463, 466, 633, 822, 861
Bme1390I CCNGG 5 cut(s) 151, 152, 404, 444, 476
Bme18I GGWCC 2 cut(s) 400, 648
BmeRI GACNNNNNGTC 2 cut(s) 373, 406
BmeT110I CYCGRG 1 cut(s) 150
BmgBI CACGTC 2 cut(s) 91, 420
BmgT120I GGNCC 5 cut(s) 400, 605, 606, 648, 818
BmiI GGNNCC 3 cut(s) 39, 607, 649
BmrFI CCNGG 5 cut(s) 151, 152, 404, 444, 476
BpiI GAAGAC 3 cut(s) 59, 140, 414
BplI GAGNNNNNCTC 2 cut(s) 278, 310
Bpu10I CCTNAGC 3 cut(s) 336, 643, 863
Bpu1102I GCTNAGC 1 cut(s) 530
Bpu14I TTCGAA 1 cut(s) 226
BpuEI CTTGAG 1 cut(s) 175
BpuMI CCSGG 3 cut(s) 151, 152, 444
BsaHI GRCGYC 3 cut(s) 38, 369, 407
BsaJI CCNNGG 1 cut(s) 150
Bsc4I CCNNNNNNNGG 2 cut(s) 67, 481
Bse1I ACTGG 1 cut(s) 61
BseBI CCWGG 2 cut(s) 404, 476
BseDI CCNNGG 1 cut(s) 150
BseGI GGATG 1 cut(s) 76
BseLI CCNNNNNNNGG 2 cut(s) 67, 481
BseMII CTCAG 5 cut(s) 344, 350, 521, 678, 854
BseNI ACTGG 1 cut(s) 61
BseRI GAGGAG 2 cut(s) 735, 788
BseSI GKGCMC 1 cut(s) 609
BseXI GCAGC 2 cut(s) 276, 359
Bsh1236I CGCG 2 cut(s) 257, 857
BshFI GGCC 3 cut(s) 80, 607, 820
BshNI GGYRCC 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 536
BsiHKCI CYCGRG 1 cut(s) 150
BslFI GGGAC 1 cut(s) 127
BslI CCNNNNNNNGG 2 cut(s) 67, 481
BsmAI GTCTC 3 cut(s) 360, 449, 518
BsmBI CGTCTC 2 cut(s) 360, 518
BsmFI GGGAC 1 cut(s) 127
BsnI GGCC 3 cut(s) 80, 607, 820
BsoBI CYCGRG 1 cut(s) 150
Bsp119I TTCGAA 1 cut(s) 226
Bsp120I GGGCCC 1 cut(s) 605
Bsp1286I GDGCHC 2 cut(s) 536, 609
Bsp143I GATC 7 cut(s) 9, 73, 300, 446, 610, 615, 667
Bsp1720I GCTNAGC 1 cut(s) 530
Bsp68I TCGCGA 1 cut(s) 257
BspANI GGCC 3 cut(s) 80, 607, 820
BspCNI CTCAG 5 cut(s) 343, 349, 522, 679, 855
BspFNI CGCG 2 cut(s) 257, 857
BspLI GGNNCC 3 cut(s) 39, 607, 649
BspPI GGATC 3 cut(s) 68, 295, 454
BspQI GCTCTTC 1 cut(s) 863
BspT104I TTCGAA 1 cut(s) 226
BspT107I GGYRCC 1 cut(s) 37
BsrBI CCGCTC 1 cut(s) 873
BsrI ACTGG 1 cut(s) 61
BssECI CCNNGG 1 cut(s) 150
BssMI GATC 7 cut(s) 9, 73, 300, 446, 610, 615, 667
BssNI GRCGYC 3 cut(s) 38, 369, 407
Bst2UI CCWGG 2 cut(s) 404, 476
Bst4CI ACNGT 3 cut(s) 139, 235, 741
Bst6I CTCTTC 2 cut(s) 720, 863
BstACI GRCGYC 3 cut(s) 38, 369, 407
BstBI TTCGAA 1 cut(s) 226
BstC8I GCNNGC 4 cut(s) 82, 206, 543, 825
BstDEI CTNAG 6 cut(s) 330, 336, 530, 643, 687, 863
BstF5I GGATG 1 cut(s) 76
BstFNI CGCG 2 cut(s) 257, 857
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 2 cut(s) 40, 859
BstKTI GATC 7 cut(s) 12, 76, 303, 449, 613, 618, 670
BstMAI GTCTC 3 cut(s) 360, 449, 518
BstMBI GATC 7 cut(s) 9, 73, 300, 446, 610, 615, 667
BstMWI GCNNNNNNNGC 2 cut(s) 201, 347
BstNI CCWGG 2 cut(s) 404, 476
BstSCI CCNGG 5 cut(s) 149, 150, 402, 442, 474
BstSLI GKGCMC 1 cut(s) 609
BstUI CGCG 2 cut(s) 257, 857
BstV1I GCAGC 2 cut(s) 276, 359
BstV2I GAAGAC 3 cut(s) 59, 140, 414
BstX2I RGATCY 1 cut(s) 300
BstYI RGATCY 1 cut(s) 300
BsuRI GGCC 3 cut(s) 80, 607, 820
BtrI CACGTC 2 cut(s) 91, 420
BtsCI GGATG 1 cut(s) 76
BtsI GCAGTG 1 cut(s) 566
BtsIMutI CAGTG 2 cut(s) 566, 684
BtuMI TCGCGA 1 cut(s) 257
Cac8I GCNNGC 4 cut(s) 82, 206, 543, 825
CfoI GCGC 2 cut(s) 40, 859
Cfr13I GGNCC 5 cut(s) 400, 605, 606, 648, 818
Cfr9I CCCGGG 1 cut(s) 150
CseI GACGC 3 cut(s) 377, 396, 535
Csp6I GTAC 1 cut(s) 592
CviAII CATG 1 cut(s) 595
CviQI GTAC 1 cut(s) 592
DdeI CTNAG 6 cut(s) 330, 336, 530, 643, 687, 863
DinI GGCGCC 1 cut(s) 39
DpnI GATC 7 cut(s) 11, 75, 302, 448, 612, 617, 669
DpnII GATC 7 cut(s) 9, 73, 300, 446, 610, 615, 667
DriI GACNNNNNGTC 2 cut(s) 373, 406
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 2 cut(s) 720, 863
Eam1105I GACNNNNNGTC 2 cut(s) 373, 406
EarI CTCTTC 2 cut(s) 720, 863
EciI GGCGGA 2 cut(s) 378, 860
Ecl136II GAGCTC 1 cut(s) 534
Eco24I GRGCYC 2 cut(s) 536, 609
Eco32I GATATC 1 cut(s) 96
Eco47I GGWCC 2 cut(s) 400, 648
Eco53kI GAGCTC 1 cut(s) 534
Eco57I CTGAAG 2 cut(s) 530, 776
Eco88I CYCGRG 1 cut(s) 150
EcoICRI GAGCTC 1 cut(s) 534
EcoO109I RGGNCCY 1 cut(s) 400
EcoRII CCWGG 2 cut(s) 402, 474
EcoRV GATATC 1 cut(s) 96
EcoT38I GRGCYC 2 cut(s) 536, 609
EgeI GGCGCC 1 cut(s) 39
EheI GGCGCC 1 cut(s) 39
Esp3I CGTCTC 2 cut(s) 360, 518
FaeI CATG 1 cut(s) 598
FaiI YATR 4 cut(s) 158, 219, 596, 629
FaqI GGGAC 1 cut(s) 127
FatI CATG 1 cut(s) 594
FauI CCCGC 2 cut(s) 709, 866
FbaI TGATCA 1 cut(s) 9
Fnu4HI GCNGC 7 cut(s) 290, 348, 462, 465, 632, 821, 860
FokI GGATG 1 cut(s) 83
FriOI GRGCYC 2 cut(s) 536, 609
Fsp4HI GCNGC 7 cut(s) 290, 348, 462, 465, 632, 821, 860
GlaI GCGC 2 cut(s) 39, 858
GluI GCNGC 7 cut(s) 290, 348, 462, 465, 632, 821, 860
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 3 cut(s) 80, 607, 820
HgaI GACGC 3 cut(s) 377, 396, 535
HhaI GCGC 2 cut(s) 40, 859
Hin1I GRCGYC 3 cut(s) 38, 369, 407
Hin1II CATG 1 cut(s) 598
Hin6I GCGC 2 cut(s) 38, 857
HinP1I GCGC 2 cut(s) 38, 857
HincII GTYRAC 2 cut(s) 378, 411
HindII GTYRAC 2 cut(s) 378, 411
HindIII AAGCTT 2 cut(s) 193, 640
HinfI GANTC 2 cut(s) 431, 439
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 3 cut(s) 378, 411, 484
Hpy188I TCNGA 6 cut(s) 48, 298, 538, 585, 615, 624
Hpy188III TCNNGA 1 cut(s) 256
Hpy8I GTNNAC 3 cut(s) 378, 411, 484
Hpy99I CGWCG 3 cut(s) 89, 92, 309
HpyAV CCTTC 4 cut(s) 277, 592, 751, 871
HpyCH4III ACNGT 3 cut(s) 139, 235, 741
HpyCH4IV ACGT 3 cut(s) 56, 90, 419
HpyCH4V TGCA 3 cut(s) 4, 545, 885
HpyF10VI GCNNNNNNNGC 2 cut(s) 201, 347
HpyF3I CTNAG 6 cut(s) 330, 336, 530, 643, 687, 863
HpySE526I ACGT 3 cut(s) 56, 90, 419
Hsp92I GRCGYC 3 cut(s) 38, 369, 407
Hsp92II CATG 1 cut(s) 598
HspAI GCGC 2 cut(s) 38, 857
KasI GGCGCC 1 cut(s) 37
Ksp22I TGATCA 1 cut(s) 9
Kzo9I GATC 7 cut(s) 9, 73, 300, 446, 610, 615, 667
LguI GCTCTTC 1 cut(s) 863
LmnI GCTCC 2 cut(s) 286, 538
Lsp1109I GCAGC 2 cut(s) 276, 359
MaeII ACGT 3 cut(s) 56, 90, 419
MaeIII GTNAC 1 cut(s) 680
MalI GATC 7 cut(s) 11, 75, 302, 448, 612, 617, 669
MbiI CCGCTC 1 cut(s) 873
MboI GATC 7 cut(s) 9, 73, 300, 446, 610, 615, 667
MflI RGATCY 1 cut(s) 300
MhlI GDGCHC 2 cut(s) 536, 609
MluCI AATT 1 cut(s) 166
Mly113I GGCGCC 1 cut(s) 38
MlyI GAGTC 1 cut(s) 425
MmeI TCCRAC 1 cut(s) 411
MnlI CCTC 7 cut(s) 269, 391, 713, 721, 766, 783, 858
MroXI GAANNNNTTC 1 cut(s) 56
MseI TTAA 1 cut(s) 210
MspA1I CMGCKG 4 cut(s) 350, 461, 654, 862
MspR9I CCNGG 5 cut(s) 151, 152, 404, 444, 476
MvaI CCWGG 2 cut(s) 404, 476
MvnI CGCG 2 cut(s) 257, 857
MwoI GCNNNNNNNGC 2 cut(s) 201, 347
NarI GGCGCC 1 cut(s) 38
NciI CCSGG 3 cut(s) 151, 152, 444
NdeII GATC 7 cut(s) 9, 73, 300, 446, 610, 615, 667
NlaIII CATG 1 cut(s) 598
NlaIV GGNNCC 3 cut(s) 39, 607, 649
NmuCI GTSAC 1 cut(s) 680
NruI TCGCGA 1 cut(s) 257
NspV TTCGAA 1 cut(s) 226
PciSI GCTCTTC 1 cut(s) 863
PcsI WCGNNNNNNNCGW 1 cut(s) 431
PdmI GAANNNNTTC 1 cut(s) 56
PfeI GAWTC 1 cut(s) 439
PflMI CCANNNNNTGG 2 cut(s) 67, 481
PfoI TCCNGGA 1 cut(s) 442
PkrI GCNGC 7 cut(s) 291, 349, 463, 466, 633, 822, 861
PleI GAGTC 1 cut(s) 425
PluTI GGCGCC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 425
PpuMI RGGWCCY 1 cut(s) 400
Psp124BI GAGCTC 1 cut(s) 536
Psp5II RGGWCCY 1 cut(s) 400
Psp6I CCWGG 2 cut(s) 402, 474
PspGI CCWGG 2 cut(s) 402, 474
PspN4I GGNNCC 3 cut(s) 39, 607, 649
PspOMI GGGCCC 1 cut(s) 605
PspPI GGNCC 5 cut(s) 400, 605, 606, 648, 818
PspPPI RGGWCCY 1 cut(s) 400
PsuI RGATCY 1 cut(s) 300
PvuII CAGCTG 1 cut(s) 654
RruI TCGCGA 1 cut(s) 257
RsaI GTAC 1 cut(s) 593
RsaNI GTAC 1 cut(s) 592
SacI GAGCTC 1 cut(s) 536
SapI GCTCTTC 1 cut(s) 863
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 7 cut(s) 290, 348, 462, 465, 632, 821, 860
Sau3AI GATC 7 cut(s) 9, 73, 300, 446, 610, 615, 667
Sau96I GGNCC 5 cut(s) 400, 605, 606, 648, 818
SchI GAGTC 1 cut(s) 425
ScrFI CCNGG 5 cut(s) 151, 152, 404, 444, 476
SduI GDGCHC 2 cut(s) 536, 609
SfoI GGCGCC 1 cut(s) 39
SfuI TTCGAA 1 cut(s) 226
SinI GGWCC 2 cut(s) 400, 648
SmaI CCCGGG 1 cut(s) 152
SmlI CTYRAG 1 cut(s) 190
SmoI CTYRAG 1 cut(s) 190
Sse9I AATT 1 cut(s) 166
SspDI GGCGCC 1 cut(s) 37
SstI GAGCTC 1 cut(s) 536
StyD4I CCNGG 5 cut(s) 149, 150, 402, 442, 474
TaaI ACNGT 3 cut(s) 139, 235, 741
TaiI ACGT 3 cut(s) 59, 93, 422
TaqI TCGA 2 cut(s) 226, 434
TasI AATT 1 cut(s) 166
TatI WGTACW 1 cut(s) 591
TauI GCSGC 5 cut(s) 464, 467, 634, 823, 862
TfiI GAWTC 1 cut(s) 439
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 2 cut(s) 566, 691
TseFI GTSAC 1 cut(s) 680
TseI GCWGC 2 cut(s) 289, 347
Tsp45I GTSAC 1 cut(s) 680
TspDTI ATGAA 3 cut(s) 65, 611, 851
TspGWI ACGGA 2 cut(s) 120, 293
TspMI CCCGGG 1 cut(s) 150
TspRI CASTG 2 cut(s) 566, 691
Van91I CCANNNNNTGG 2 cut(s) 67, 481
VpaK11BI GGWCC 2 cut(s) 400, 648
XmaI CCCGGG 1 cut(s) 150
XmnI GAANNNNTTC 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.