Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011222

Physical Location & Seq

Genomic Coordinates Reverse (-)

30028734 .. 30030016

Gene Structure 1283 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1305700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1236 bp

ATGAGCTACGAGACGCACGCGGTGGTTCTGAAGCTCAAAACACCGCAAACCAATGAGTCCAAGACGGCGTCGTTAGAGCTCCGTGCTCCGATTGACTTGGTGACGGTGGTGGACGTGAACGTGAGCACGAGCAATGCGAGGATTCAGATGATGAAGCGCACGATGCGACTTGTAGCATATTCCTCCACTTCGAAGAGGTTGTTGCCGCTCCGGCGAATGACCTTCGTCGGTCGACGTTCGGCTCGTCGGATTATGGATGCGCTATGTGCCCTCGGTCAGAGGATCTGTCTCAACGACGCGCTTAAGAAGGCCGCAAAGGTGATCCAAGATCGGCGCGAGAAAAATGCTGTTGCGAGTATCATACTTCTATTGAATGCCGCTCAACCAAAAACCAATCAGAAGCGATCTTCTTCCCCTATCGTGTCTTCCACGCGTTTCTCTCACCTCGACATCCCCGTCCACACCGTCGCAGTCGGGGACGGTTGCCACAACGCACTTGTAAACGTCGTGGTCCAGGATCTTTGTCTCCAACTCAGCGTCATTTTGGGTTCATTACCGACCAAGATCGCTGCTATCTACTCACTCACGAGTGGGCCCGCGGCTCTAGGATCCGTTTCGATCCGACTCGGTGACTTCTACGCCGGAGAGGAACGAGAGTTATTAGTCGAACTGAAAATGTCGTTTTCCGCCATGGCAGGGCCTCACGCATCCCAAAATGTGATATCTGTGAGGTCCACACACAGGGTCTCGTCGTCCAATGAGTTTGTGTGTTCAAAAGAGCGGGTCCTCCTCCTACCTCGATCGCAGACCGTCCGATCGTCACCGTTGAACTCGAACATCGAGCGGTTGAAGAACCTCCACGTAGTGGCTAGAGCCGTGGCGGAGTCGCGGAGGTTTATGGAGAGGAGCAGCGACTTTTCGAGCGCGTACCACCTGCTGTCGTCGGCGCGAACGTTGCTCCTGCATTCGAGCTCAGCTTCGGTAGAGGAGTTCCTGCGTGGTTTGGAGGGGGAGATGGCTGAGTTGCAGAGGCGGCGGCAGGTTCAGCTGCTGGCGAAGGTTGAGGCGAAGCAAAGCAAGAAAAGATTCAACAACGGACAGGTGGAGGACAAGCAGGAGCCGCTAACGCCGACGTTGGCTTGGCGCGCCGCTGAGAAATTGGCTAAGGTGGCTACGTTAAGGAAGTCCATGAACAGAGTTAGCGACTTACACGGCTTCGAAAATGCCAGATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000741 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006807 GO:0006996 GO:0006997 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009553 GO:0009559 GO:0009561 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009987 GO:0010197 GO:0016020 GO:0016043 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022622 GO:0030054 GO:0030154 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048229 GO:0048284 GO:0048364 GO:0048731 GO:0048856 GO:0048869 GO:0050896 GO:0055044 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0099402 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

VWA VWA_2 VWA_3 zf-RING_11

Protein Analysis

412

Amino Acids

45.52

Weight (kDa)

10.35

Isoelectric Point (pI)

56.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WAV3_C	PF25243	278 - 349	4.2e-29	E3 ubiquitin-protein ligase WAV3-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22680 AT4G37890 AT4G37890
fragaria_vesca	FvH4_1g18891
malus_domestica	MD01G1045900.v1.1 MD15G1305700.v1.1
prunus_persica	Prupe.6G210600_v2.0.a1
pyrus_communis	pycom01g07210 pycom15g26960
rosa_chinensis	RchiOBHm_Chr2g0109851
rosa_laevigata	RLG00000017767
rosa_multiflora	Rmu_co8072298.1_g000001 Rmu_co8290675.1_g000001 Rmu_sc0008299.1_g000001
rosa_roxburghii	Rroxscaffold_2G00133910
rosa_samantha	Rh2AG215300 Rh2BG225400 Rh2CG217200 Rh2DG220800
rosa_wichuraiana	Rw2G016630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	942
AasI	GACNNNNNNGTC	1 cut(s)	455
Acc36I	ACCTGC	2 cut(s)	942, 1030
AccB7I	CCANNNNNTGG	1 cut(s)	865
AccBSI	CCGCTC	4 cut(s)	208, 380, 781, 844
AccI	GTMKAC	1 cut(s)	232
AccII	CGCG	9 cut(s)	20, 299, 336, 433, 599, 889, 926, 949, 1146
AclI	AACGTT	1 cut(s)	953
AclWI	GGATC	6 cut(s)	290, 316, 525, 603, 613, 616
AcuI	CTGAAG	1 cut(s)	50
AcyI	GRCGYC	1 cut(s)	68
AdeI	CACNNNGTG	2 cut(s)	22, 865
AfaI	GTAC	1 cut(s)	929
AfiI	CCNNNNNNNGG	3 cut(s)	696, 741, 865
AflII	CTTAAG	1 cut(s)	302
AflIII	ACRYGT	1 cut(s)	431
AgsI	TTSAA	5 cut(s)	373, 774, 829, 850, 1090
AjiI	CACGTC	1 cut(s)	115
AjnI	CCWGG	1 cut(s)	513
AluBI	AGCT	6 cut(s)	6, 34, 79, 972, 977, 1048
AluI	AGCT	6 cut(s)	6, 34, 79, 972, 977, 1048
Alw21I	GWGCWC	4 cut(s)	81, 88, 128, 974
Alw26I	GTCTC	4 cut(s)	5, 293, 530, 751
AlwI	GGATC	6 cut(s)	290, 316, 525, 603, 613, 616
AlwNI	CAGNNNCTG	1 cut(s)	1051
AoxI	GGCC	3 cut(s)	309, 593, 698
ApaI	GGGCCC	1 cut(s)	597
ApeKI	GCWGC	3 cut(s)	569, 909, 1048
AscI	GGCGCGCC	1 cut(s)	1144
Asp700I	GAANNNNTTC	1 cut(s)	1085
AspLEI	GCGC	8 cut(s)	159, 262, 301, 336, 926, 949, 1146, 1148
AspS9I	GGNCC	6 cut(s)	511, 593, 594, 698, 732, 784
AsuHPI	GGTGA	5 cut(s)	112, 331, 434, 641, 813
AsuII	TTCGAA	2 cut(s)	191, 1218
AvaII	GGWCC	3 cut(s)	511, 732, 784
BaeGI	GKGCMC	2 cut(s)	271, 597
BamHI	GGATCC	1 cut(s)	608
BanII	GRGCYC	3 cut(s)	81, 597, 974
BauI	CACGAG	2 cut(s)	127, 586
BbsI	GAAGAC	1 cut(s)	417
Bbv12I	GWGCWC	4 cut(s)	81, 88, 128, 974
BbvI	GCAGC	3 cut(s)	556, 921, 1035
BccI	CCATC	1 cut(s)	1009
BceAI	ACGGC	3 cut(s)	81, 860, 1228
BcgI	CGANNNNNNTGC	2 cut(s)	326, 360
BciT130I	CCWGG	1 cut(s)	515
BcoDI	GTCTC	4 cut(s)	5, 293, 530, 751
BfaI	CTAG	2 cut(s)	605, 870
BfrI	CTTAAG	1 cut(s)	302
BfuAI	ACCTGC	2 cut(s)	942, 1030
BglI	GCCNNNNNGGC	1 cut(s)	211
BlpI	GCTNAGC	1 cut(s)	973
Bme1390I	CCNGG	1 cut(s)	515
Bme18I	GGWCC	3 cut(s)	511, 732, 784
BmgBI	CACGTC	1 cut(s)	115
BmgT120I	GGNCC	6 cut(s)	511, 593, 594, 698, 732, 784
BmiI	GGNNCC	4 cut(s)	595, 610, 785, 1119
BmrFI	CCNGG	1 cut(s)	515
BmsI	GCATC	3 cut(s)	153, 247, 716
BoxI	GACNNNNGTC	1 cut(s)	224
BpiI	GAAGAC	1 cut(s)	417
Bpu10I	CCTNAGC	1 cut(s)	1164
Bpu1102I	GCTNAGC	1 cut(s)	973
Bpu14I	TTCGAA	2 cut(s)	191, 1218
BsaAI	YACGTR	1 cut(s)	862
BsaHI	GRCGYC	1 cut(s)	68
BsaI	GGTCTC	1 cut(s)	751
BsaJI	CCNNGG	4 cut(s)	271, 597, 690, 876
Bsc4I	CCNNNNNNNGG	3 cut(s)	696, 741, 865
Bse3DI	GCAATG	1 cut(s)	139
BseBI	CCWGG	1 cut(s)	515
BseDI	CCNNGG	4 cut(s)	271, 597, 690, 876
BseGI	GGATG	3 cut(s)	262, 450, 707
BseLI	CCNNNNNNNGG	3 cut(s)	696, 741, 865
BseMI	GCAATG	1 cut(s)	139
BseMII	CTCAG	4 cut(s)	547, 987, 1011, 1143
BsePI	GCGCGC	1 cut(s)	1144
BseRI	GAGGAG	3 cut(s)	779, 919, 1001
BseSI	GKGCMC	2 cut(s)	271, 597
BseXI	GCAGC	3 cut(s)	556, 921, 1035
Bsh1236I	CGCG	9 cut(s)	20, 299, 336, 433, 599, 889, 926, 949, 1146
Bsh1285I	CGRYCG	3 cut(s)	232, 803, 818
BshFI	GGCC	3 cut(s)	311, 595, 700
BsiEI	CGRYCG	3 cut(s)	232, 803, 818
BsiHKAI	GWGCWC	4 cut(s)	81, 88, 128, 974
BsiSI	CCGG	2 cut(s)	211, 642
BslFI	GGGAC	1 cut(s)	491
BslI	CCNNNNNNNGG	3 cut(s)	696, 741, 865
BsmAI	GTCTC	4 cut(s)	5, 293, 530, 751
BsmBI	CGTCTC	1 cut(s)	5
BsmFI	GGGAC	1 cut(s)	491
BsmI	GAATGC	2 cut(s)	379, 964
BsnI	GGCC	3 cut(s)	311, 595, 700
Bso31I	GGTCTC	1 cut(s)	751
Bsp119I	TTCGAA	2 cut(s)	191, 1218
Bsp120I	GGGCCC	1 cut(s)	593
Bsp1286I	GDGCHC	6 cut(s)	81, 88, 128, 271, 597, 974
Bsp1720I	GCTNAGC	1 cut(s)	973
Bsp19I	CCATGG	1 cut(s)	690
BspANI	GGCC	3 cut(s)	311, 595, 700
BspCNI	CTCAG	4 cut(s)	546, 986, 1012, 1144
BspFNI	CGCG	9 cut(s)	20, 299, 336, 433, 599, 889, 926, 949, 1146
BspLI	GGNNCC	4 cut(s)	595, 610, 785, 1119
BspMI	ACCTGC	2 cut(s)	942, 1030
BspPI	GGATC	6 cut(s)	290, 316, 525, 603, 613, 616
BspT104I	TTCGAA	2 cut(s)	191, 1218
BspTI	CTTAAG	1 cut(s)	302
BspTNI	GGTCTC	1 cut(s)	751
BsrBI	CCGCTC	4 cut(s)	208, 380, 781, 844
BsrDI	GCAATG	1 cut(s)	139
BssECI	CCNNGG	4 cut(s)	271, 597, 690, 876
BssHII	GCGCGC	1 cut(s)	1144
BssNI	GRCGYC	1 cut(s)	68
BssSI	CACGAG	2 cut(s)	127, 586
BssT1I	CCWWGG	1 cut(s)	690
Bst2BI	CACGAG	2 cut(s)	127, 586
Bst2UI	CCWGG	1 cut(s)	515
Bst4CI	ACNGT	5 cut(s)	106, 466, 482, 811, 825
Bst6I	CTCTTC	1 cut(s)	188
BstACI	GRCGYC	1 cut(s)	68
BstAFI	CTTAAG	1 cut(s)	302
BstBAI	YACGTR	1 cut(s)	862
BstBI	TTCGAA	2 cut(s)	191, 1218
BstC8I	GCNNGC	4 cut(s)	18, 597, 1053, 1146
BstDEI	CTNAG	5 cut(s)	533, 973, 1020, 1152, 1164
BstDSI	CCRYGG	3 cut(s)	597, 690, 876
BstF5I	GGATG	3 cut(s)	262, 450, 707
BstFNI	CGCG	9 cut(s)	20, 299, 336, 433, 599, 889, 926, 949, 1146
BstHHI	GCGC	8 cut(s)	159, 262, 301, 336, 926, 949, 1146, 1148
BstMAI	GTCTC	4 cut(s)	5, 293, 530, 751
BstMCI	CGRYCG	3 cut(s)	232, 803, 818
BstNI	CCWGG	1 cut(s)	515
BstPAI	GACNNNNGTC	1 cut(s)	224
BstSCI	CCNGG	1 cut(s)	513
BstSLI	GKGCMC	2 cut(s)	271, 597
BstUI	CGCG	9 cut(s)	20, 299, 336, 433, 599, 889, 926, 949, 1146
BstV1I	GCAGC	3 cut(s)	556, 921, 1035
BstV2I	GAAGAC	1 cut(s)	417
BstX2I	RGATCY	3 cut(s)	282, 517, 608
BstYI	RGATCY	3 cut(s)	282, 517, 608
BsuRI	GGCC	3 cut(s)	311, 595, 700
BtgI	CCRYGG	3 cut(s)	597, 690, 876
BtrI	CACGTC	1 cut(s)	115
BtsCI	GGATG	3 cut(s)	262, 450, 707
BveI	ACCTGC	2 cut(s)	942, 1030
Cac8I	GCNNGC	4 cut(s)	18, 597, 1053, 1146
CaiI	CAGNNNCTG	1 cut(s)	1051
CfoI	GCGC	8 cut(s)	159, 262, 301, 336, 926, 949, 1146, 1148
Cfr13I	GGNCC	6 cut(s)	511, 593, 594, 698, 732, 784
Cfr42I	CCGCGG	1 cut(s)	600
CseI	GACGC	4 cut(s)	22, 57, 305, 526
Csp6I	GTAC	1 cut(s)	928
CviAII	CATG	2 cut(s)	691, 1189
CviQI	GTAC	1 cut(s)	928
DdeI	CTNAG	5 cut(s)	533, 973, 1020, 1152, 1164
DraIII	CACNNNGTG	2 cut(s)	22, 865
DrdI	GACNNNNNNGTC	1 cut(s)	455
DseDI	GACNNNNNNGTC	1 cut(s)	455
Eam1104I	CTCTTC	1 cut(s)	188
EarI	CTCTTC	1 cut(s)	188
EciI	GGCGGA	2 cut(s)	676, 896
Ecl136II	GAGCTC	2 cut(s)	79, 972
Eco130I	CCWWGG	1 cut(s)	690
Eco24I	GRGCYC	3 cut(s)	81, 597, 974
Eco31I	GGTCTC	1 cut(s)	751
Eco32I	GATATC	1 cut(s)	723
Eco47I	GGWCC	3 cut(s)	511, 732, 784
Eco53kI	GAGCTC	2 cut(s)	79, 972
Eco57I	CTGAAG	1 cut(s)	50
EcoICRI	GAGCTC	2 cut(s)	79, 972
EcoO109I	RGGNCCY	2 cut(s)	698, 784
EcoRII	CCWGG	1 cut(s)	513
EcoRV	GATATC	1 cut(s)	723
EcoT14I	CCWWGG	1 cut(s)	690
EcoT38I	GRGCYC	3 cut(s)	81, 597, 974
ErhI	CCWWGG	1 cut(s)	690
Esp3I	CGTCTC	1 cut(s)	5
FaeI	CATG	2 cut(s)	694, 1192
FaiI	YATR	7 cut(s)	178, 254, 265, 362, 692, 899, 1190
FaqI	GGGAC	1 cut(s)	491
FatI	CATG	2 cut(s)	690, 1188
FauI	CCCGC	2 cut(s)	604, 774
FblI	GTMKAC	1 cut(s)	232
FokI	GGATG	3 cut(s)	269, 437, 694
FriOI	GRGCYC	3 cut(s)	81, 597, 974
FspBI	CTAG	2 cut(s)	605, 870
GlaI	GCGC	8 cut(s)	158, 261, 300, 335, 925, 948, 1145, 1147
HaeIII	GGCC	3 cut(s)	311, 595, 700
HapII	CCGG	2 cut(s)	211, 642
HgaI	GACGC	4 cut(s)	22, 57, 305, 526
HhaI	GCGC	8 cut(s)	159, 262, 301, 336, 926, 949, 1146, 1148
Hin1I	GRCGYC	1 cut(s)	68
Hin1II	CATG	2 cut(s)	694, 1192
Hin6I	GCGC	8 cut(s)	157, 260, 299, 334, 924, 947, 1144, 1146
HinP1I	GCGC	8 cut(s)	157, 260, 299, 334, 924, 947, 1144, 1146
HincII	GTYRAC	1 cut(s)	233
HindII	GTYRAC	1 cut(s)	233
HinfI	GANTC	5 cut(s)	56, 142, 624, 884, 1086
HpaII	CCGG	2 cut(s)	211, 642
HphI	GGTGA	5 cut(s)	112, 331, 434, 641, 813
Hpy166II	GTNNAC	6 cut(s)	112, 118, 233, 460, 502, 735
Hpy188I	TCNGA	8 cut(s)	30, 90, 147, 249, 279, 399, 623, 815
Hpy188III	TCNNGA	1 cut(s)	586
Hpy8I	GTNNAC	6 cut(s)	112, 118, 233, 460, 502, 735
HpyAV	CCTTC	3 cut(s)	232, 301, 1051
HpyCH4III	ACNGT	5 cut(s)	106, 466, 482, 811, 825
HpyCH4IV	ACGT	8 cut(s)	114, 120, 235, 504, 861, 953, 1133, 1175
HpyCH4V	TGCA	2 cut(s)	964, 1027
HpyF3I	CTNAG	5 cut(s)	533, 973, 1020, 1152, 1164
HpySE526I	ACGT	8 cut(s)	114, 120, 235, 504, 861, 953, 1133, 1175
Hsp92I	GRCGYC	1 cut(s)	68
Hsp92II	CATG	2 cut(s)	694, 1192
HspAI	GCGC	8 cut(s)	157, 260, 299, 334, 924, 947, 1144, 1146
KspI	CCGCGG	1 cut(s)	600
LmnI	GCTCC	6 cut(s)	84, 91, 213, 906, 963, 1117
Lsp1109I	GCAGC	3 cut(s)	556, 921, 1035
LweI	GCATC	3 cut(s)	153, 247, 716
MaeI	CTAG	2 cut(s)	605, 870
MaeII	ACGT	8 cut(s)	114, 120, 235, 504, 861, 953, 1133, 1175
MaeIII	GTNAC	3 cut(s)	100, 629, 819
MbiI	CCGCTC	4 cut(s)	208, 380, 781, 844
MboII	GAAGA	5 cut(s)	205, 399, 402, 417, 862
MflI	RGATCY	3 cut(s)	282, 517, 608
MhlI	GDGCHC	6 cut(s)	81, 88, 128, 271, 597, 974
MluCI	AATT	1 cut(s)	1157
MluI	ACGCGT	1 cut(s)	431
MlyI	GAGTC	3 cut(s)	65, 618, 893
MmeI	TCCRAC	3 cut(s)	227, 553, 646
MroXI	GAANNNNTTC	1 cut(s)	1085
MseI	TTAA	2 cut(s)	303, 1178
MspA1I	CMGCKG	3 cut(s)	599, 1048, 1151
MspCI	CTTAAG	1 cut(s)	302
MspI	CCGG	2 cut(s)	211, 642
MspR9I	CCNGG	1 cut(s)	515
Mva1269I	GAATGC	2 cut(s)	379, 964
MvaI	CCWGG	1 cut(s)	515
MvnI	CGCG	9 cut(s)	20, 299, 336, 433, 599, 889, 926, 949, 1146
NcoI	CCATGG	1 cut(s)	690
NlaIII	CATG	2 cut(s)	694, 1192
NlaIV	GGNNCC	4 cut(s)	595, 610, 785, 1119
NmuCI	GTSAC	3 cut(s)	100, 629, 819
NspV	TTCGAA	2 cut(s)	191, 1218
PalAI	GGCGCGCC	1 cut(s)	1144
PaqCI	CACCTGC	1 cut(s)	942
PauI	GCGCGC	1 cut(s)	1144
PcsI	WCGNNNNNNNCGW	4 cut(s)	134, 241, 453, 950
PctI	GAATGC	2 cut(s)	379, 964
PdmI	GAANNNNTTC	1 cut(s)	1085
PfeI	GAWTC	2 cut(s)	142, 1086
PflFI	GACNNNGTC	1 cut(s)	67
PflMI	CCANNNNNTGG	1 cut(s)	865
PfoI	TCCNGGA	1 cut(s)	513
Ple19I	CGATCG	2 cut(s)	803, 818
PleI	GAGTC	3 cut(s)	64, 618, 892
PpsI	GAGTC	3 cut(s)	64, 618, 892
Ppu21I	YACGTR	1 cut(s)	862
PpuMI	RGGWCCY	1 cut(s)	784
PshAI	GACNNNNGTC	1 cut(s)	224
Psp124BI	GAGCTC	2 cut(s)	81, 974
Psp1406I	AACGTT	1 cut(s)	953
Psp5II	RGGWCCY	1 cut(s)	784
Psp6I	CCWGG	1 cut(s)	513
PspGI	CCWGG	1 cut(s)	513
PspN4I	GGNNCC	4 cut(s)	595, 610, 785, 1119
PspOMI	GGGCCC	1 cut(s)	593
PspPI	GGNCC	6 cut(s)	511, 593, 594, 698, 732, 784
PspPPI	RGGWCCY	1 cut(s)	784
PstNI	CAGNNNCTG	1 cut(s)	1051
PsuI	RGATCY	3 cut(s)	282, 517, 608
PsyI	GACNNNGTC	1 cut(s)	67
PteI	GCGCGC	1 cut(s)	1144
PvuI	CGATCG	2 cut(s)	803, 818
PvuII	CAGCTG	1 cut(s)	1048
RsaI	GTAC	1 cut(s)	929
RsaNI	GTAC	1 cut(s)	928
SacI	GAGCTC	2 cut(s)	81, 974
SacII	CCGCGG	1 cut(s)	600
SalI	GTCGAC	1 cut(s)	231
SaqAI	TTAA	2 cut(s)	303, 1178
Sau96I	GGNCC	6 cut(s)	511, 593, 594, 698, 732, 784
SchI	GAGTC	3 cut(s)	65, 618, 893
ScrFI	CCNGG	1 cut(s)	515
SduI	GDGCHC	6 cut(s)	81, 88, 128, 271, 597, 974
SfaNI	GCATC	3 cut(s)	153, 247, 716
Sfr303I	CCGCGG	1 cut(s)	600
SfuI	TTCGAA	2 cut(s)	191, 1218
SgrBI	CCGCGG	1 cut(s)	600
SgsI	GGCGCGCC	1 cut(s)	1144
SinI	GGWCC	3 cut(s)	511, 732, 784
SmlI	CTYRAG	1 cut(s)	302
SmoI	CTYRAG	1 cut(s)	302
Sse9I	AATT	1 cut(s)	1157
SspMI	CTAG	2 cut(s)	605, 870
SstI	GAGCTC	2 cut(s)	81, 974
StyD4I	CCNGG	1 cut(s)	513
StyI	CCWWGG	1 cut(s)	690
TaaI	ACNGT	5 cut(s)	106, 466, 482, 811, 825
TaiI	ACGT	8 cut(s)	117, 123, 238, 507, 864, 956, 1136, 1178
TaqII	GACCGA	2 cut(s)	218, 263
TasI	AATT	1 cut(s)	1157
TauI	GCSGC	8 cut(s)	208, 314, 380, 602, 1036, 1039, 1123, 1151
TfiI	GAWTC	2 cut(s)	142, 1086
Tru1I	TTAA	2 cut(s)	303, 1178
Tru9I	TTAA	2 cut(s)	303, 1178
TseFI	GTSAC	3 cut(s)	100, 629, 819
TseI	GCWGC	3 cut(s)	569, 909, 1048
Tsp45I	GTSAC	3 cut(s)	100, 629, 819
TspDTI	ATGAA	3 cut(s)	167, 540, 1205
TspGWI	ACGGA	3 cut(s)	71, 601, 1110
Tth111I	GACNNNGTC	1 cut(s)	67
Van91I	CCANNNNNTGG	1 cut(s)	865
Vha464I	CTTAAG	1 cut(s)	302
VpaK11BI	GGWCC	3 cut(s)	511, 732, 784
XmiI	GTMKAC	1 cut(s)	232
XmnI	GAANNNNTTC	1 cut(s)	1085
XspI	CTAG	2 cut(s)	605, 870

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.