Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0011222

Physical Location & Seq

Genomic Coordinates Forward (+)

71434360 .. 71436984

Gene Structure 2625 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00133910.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2208 bp

ATGCTCTTCTTCCATTTTGCTAAAATCAATCCCAAATCAGAAACAGAGACAAGGAATCAAAAAATGGTGACTGGGTGGAGAAGGGCCTTCTGCACATCTATCCCTAAAGACAGACACACCAAAGTTGTAACAGAGAAGCAGCAGCAGCAGCAACAATGTGAAAACACCAACACCAACACCAACACCAACAATAACCAAAGCCCCAAAATCACCTCCAAATTCGGTTTCTTCTCATCTTCCTCAAACCCATCCACGCCTCGCCTTCAATCCCAGCCGGTCTCGACTCCAAGCCTCCGCTGCAGAACCACCGCCGCCACAGCACCCACCACCCCGAATTCTTCCCTCCCCAACAGCCCAAAACTGCAATGCAACGTCGCTGCCACCACCACAACCCCAAAAAAAATCACCTACAGCCCGAGACTGTTCCACCGATCCAACCCCTCCTCCCCGAAATCGCCTTCCAGCTTCTCCCTCCTCAAATCCACCCTCCGCCTCAACAAAAGTAGATGCGGAATCTGCTTGCGGAGCGTGAAAAGTGGTCAAGGTACGGCCATTTTCACAGCCGAGTGCTCCCACTCGTTTCACTATCCTTGCGTCGCCACCCATGTCAAGAAGCAACCTCTCATGCTCTGCCCCGTCTGCAACGCCACCTGGAAAGAGCTGCCTCTGCTCGAAACTCTCACTCCTCGCACCAATACTAAGCCCGAGTCCAAGCTCAGAGACCTCAAGACCAAACCCTTGAGGGTTTACAACGACGACGAGCCGCTCATGTCGCCCACTTCCGGCGCCAGGTTCAATCCCATTCCCGAGTCCGACGAAAACGAGGAGGAAAACGACGCTGTTGAGTTCCAGGGCTTCTTTGTCAATCCTGATAGGAATAATGTTTGTTCCGGCAATCCGAAGAGTGTGGAGTTCAGTCTGTTACCTGAATCGGTGGTCGTGGCGCTTGGGACTCACGCCGTCGTCTTGAAAGTCAAAGCTCCGCAGCTTGTGGAAGGTAGAACGACGTCGTCGCGAAGGCCGCCGATTGACTTGGTGACGGTGGTCGACGTCAGCGCCAATACCAGCCGCGCCAGGATTCAGGCCACCAAACGCGCGTTGCGGTTGATCGTTTCTTCTCTCTCCGACGCCGACAGGCTCTCCATCGTGGCCTTCTCGTCCACCTCCAAGAGGCTGCTGCCGTTACGGAGGATGACGTCGGCCGGCCGGAGATCGGCGCGTCGGATAGTCGACGCGCTCTGCGGCGTTGGCCAGGGTATGTGTGTCAACGACGCGCTCAAGAAGGCGGCGAAGGTGTTGCAAGACCGCCGGGAGAGGAACCCCGTCGCTAGCATTATGCTGCTATCAGACGGCGGCGTTTCCGCCAATCAGAAGCGCTCCTCCCCGGTCGTTTCCTCCACGCGCTTCCCCCACCTCGACATCCCCGTCCACACCGTCGGATTAACCGACACTACCGACGACGCGTTCGCTAAGTGCGTCGGGGGTTTGCTAAGCGTGGTGGTGAAAGACGTCAAGCTCCAGCTCAGCGTCGTAACCAGTTCAGCCCACGCCGAGATCGCGGCGGTTTATTCCCTCACCGGCCGGCCCACGGCTCTCGGATCCGGTTCGATCCGGCTCGGCGACCTCTACGCCGGAGAAGAGCGAGAGCTGCTCGTCGAGTTGAAAGTCCCGGTCAATTCCGGCGGGTCCCACACCCAAAACGCGATGATGTCCGTACGGTCCACCTACAGAGACCCGTCGTCGAATGAGCTCGTATTTTCCCAAGAGCGGGCCCTCCTCGTACCGCGCTCGCAAGCCGTCCGATCGTCTTCTTCCTCTTCCTCCAACCCCAACATCCAACGGCTGCGAAATCTCCACGTCACGGCTCGAGCCGTGGCCGAGTCGCGCCGGTTCTTGGAGCGAAACGACTTCTCAGGCGCCTATCACTTGCTGACTTCGGCTCGAGCTCTGCTGCTCCAGTCGAGCTCGGCTTCGGTGGAGGAGTTCCTGCGCGGCTTGGAGGCTGAGCTGGCGGAGCTGCACCGTGGAAGGCAGCACCAGAGTGTGGCCCAGAGGCAGAGAGGGAACGGACACGTGGAGGAGAAGCCGGAGCCGCTGACGCCGACCTCGGCTTGGCGCGCCGCCGAGAGATTGGCTAAGGTGGCTATAATGAGGAAGTCCATGAACAGAGTCAGCGACTTACACGGCTTCGAAAACGCCAGATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000741 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006807 GO:0006996 GO:0006997 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009553 GO:0009559 GO:0009561 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009987 GO:0010197 GO:0016020 GO:0016043 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022622 GO:0030054 GO:0030154 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048229 GO:0048284 GO:0048364 GO:0048731 GO:0048856 GO:0048869 GO:0050896 GO:0055044 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0099402 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

VWA VWA_2 VWA_3 zf-RING_11

Protein Analysis

735

Amino Acids

80.65

Weight (kDa)

10.04

Isoelectric Point (pI)

50.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	169 - 215	1.9e-07	Ring finger domain
zf-RING_11	PF17123	170 - 199	1e-08	RING-like zinc finger
VWA_3	PF13768	344 - 487	5e-13	von Willebrand factor type A domain
VWA	PF00092	344 - 488	8e-13	von Willebrand factor type A domain
VWA_2	PF13519	345 - 451	6e-16	von Willebrand factor type A domain
WAV3_C	PF25243	610 - 683	9.3e-35	E3 ubiquitin-protein ligase WAV3-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22680 AT4G37890 AT4G37890
fragaria_vesca	FvH4_1g18891
malus_domestica	MD01G1045900.v1.1 MD15G1305700.v1.1
prunus_persica	Prupe.6G210600_v2.0.a1
pyrus_communis	pycom01g07210 pycom15g26960
rosa_chinensis	RchiOBHm_Chr2g0109851
rosa_laevigata	RLG00000017767
rosa_multiflora	Rmu_co8072298.1_g000001 Rmu_co8290675.1_g000001 Rmu_sc0008299.1_g000001
rosa_roxburghii	Rroxscaffold_2G00133910
rosa_samantha	Rh2AG215300 Rh2BG225400 Rh2CG217200 Rh2DG220800
rosa_wichuraiana	Rw2G016630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1424
AatII	GACGTC	4 cut(s)	1010, 1053, 1199, 1512
AccB1I	GGYRCC	2 cut(s)	785, 1916
AccB7I	CCANNNNNTGG	1 cut(s)	2044
AccBSI	CCGCTC	2 cut(s)	766, 1768
AccI	GTMKAC	2 cut(s)	1047, 1230
AclWI	GGATC	4 cut(s)	426, 1593, 1603, 1606
AcoI	YGGCCR	6 cut(s)	549, 1200, 1204, 1249, 1579, 1875
AcsI	RAATTY	2 cut(s)	218, 334
AcvI	CACGTG	1 cut(s)	2074
AcyI	GRCGYC	8 cut(s)	786, 1007, 1050, 1128, 1196, 1509, 1917, 2099
AfaI	GTAC	3 cut(s)	547, 1716, 1782
AfeI	AGCGCT	1 cut(s)	1376
AflIII	ACRYGT	2 cut(s)	1461, 2071
AgsI	TTSAA	4 cut(s)	266, 796, 970, 1663
AhdI	GACNNNNNGTC	1 cut(s)	1738
AjiI	CACGTC	1 cut(s)	1858
AjnI	CCWGG	5 cut(s)	650, 788, 849, 1073, 1251
AjuI	GAANNNNNNNTTGG	2 cut(s)	209, 241
AleI	CACNNNNGTG	1 cut(s)	2040
Alw21I	GWGCWC	4 cut(s)	572, 1752, 1948, 1967
Alw26I	GTCTC	5 cut(s)	41, 283, 412, 714, 1725
AlwI	GGATC	4 cut(s)	426, 1593, 1603, 1606
Ama87I	CYCGRG	5 cut(s)	415, 704, 806, 1866, 1941
Aor51HI	AGCGCT	1 cut(s)	1376
ApaI	GGGCCC	1 cut(s)	1774
ApoI	RAATTY	2 cut(s)	218, 334
ArsI	GACNNNNNNTTYG	2 cut(s)	1448, 1480
AscI	GGCGCGCC	1 cut(s)	2116
AspS9I	GGNCC	7 cut(s)	84, 1584, 1686, 1719, 1770, 1771, 2047
AsuC2I	CCSGG	3 cut(s)	1310, 1385, 1670
AsuHPI	GGTGA	6 cut(s)	79, 202, 397, 1048, 1513, 1567
AsuII	TTCGAA	1 cut(s)	2190
AsuNHI	GCTAGC	1 cut(s)	1328
AvaI	CYCGRG	5 cut(s)	415, 704, 806, 1866, 1941
AvaII	GGWCC	2 cut(s)	1686, 1719
BaeGI	GKGCMC	1 cut(s)	1774
BalI	TGGCCA	1 cut(s)	1251
BamHI	GGATCC	1 cut(s)	1598
BanI	GGYRCC	2 cut(s)	785, 1916
BanII	GRGCYC	4 cut(s)	1752, 1774, 1948, 1967
BbrPI	CACGTG	1 cut(s)	2074
BbsI	GAAGAC	1 cut(s)	1800
Bbv12I	GWGCWC	4 cut(s)	572, 1752, 1948, 1967
BccI	CCATC	2 cut(s)	256, 1151
BcgI	CGANNNNNNTGC	2 cut(s)	1279, 1313
BciT130I	CCWGG	5 cut(s)	652, 790, 851, 1075, 1253
BcnI	CCSGG	3 cut(s)	1310, 1385, 1670
BcoDI	GTCTC	5 cut(s)	41, 283, 412, 714, 1725
BfaI	CTAG	1 cut(s)	1329
BfmI	CTRYAG	3 cut(s)	298, 409, 1726
BfoI	RGCGCY	5 cut(s)	789, 947, 1059, 1378, 1920
BlpI	GCTNAGC	3 cut(s)	1490, 1523, 2004
Bme1390I	CCNGG	8 cut(s)	652, 790, 851, 1075, 1253, 1310, 1385, 1670
Bme18I	GGWCC	2 cut(s)	1686, 1719
BmeRI	GACNNNNNGTC	1 cut(s)	1738
BmeT110I	CYCGRG	5 cut(s)	415, 704, 806, 1866, 1941
BmgBI	CACGTC	1 cut(s)	1858
BmgT120I	GGNCC	7 cut(s)	84, 1584, 1686, 1719, 1770, 1771, 2047
BmiI	GGNNCC	8 cut(s)	787, 1319, 1600, 1687, 1688, 1772, 1918, 2091
BmrFI	CCNGG	8 cut(s)	652, 790, 851, 1075, 1253, 1310, 1385, 1670
BmrI	ACTGGG	1 cut(s)	81
BmsI	GCATC	1 cut(s)	497
BmtI	GCTAGC	1 cut(s)	1332
BmuI	ACTGGG	1 cut(s)	81
BoxI	GACNNNNGTC	1 cut(s)	1043
BpiI	GAAGAC	1 cut(s)	1800
BplI	GAGNNNNNCTC	2 cut(s)	1635, 1667
BpmI	CTGGAG	2 cut(s)	1502, 1940
Bpu10I	CCTNAGC	1 cut(s)	2136
Bpu1102I	GCTNAGC	3 cut(s)	1490, 1523, 2004
Bpu14I	TTCGAA	1 cut(s)	2190
BpuEI	CTTGAG	3 cut(s)	710, 760, 1262
BpuMI	CCSGG	3 cut(s)	1310, 1385, 1670
BsaAI	YACGTR	1 cut(s)	2074
BsaHI	GRCGYC	8 cut(s)	786, 1007, 1050, 1128, 1196, 1509, 1917, 2099
BsaI	GGTCTC	3 cut(s)	283, 714, 1725
BsaJI	CCNNGG	7 cut(s)	850, 1252, 1383, 1587, 1872, 2023, 2106
BsaWI	WCCGGW	1 cut(s)	1601
BsaXI	ACNNNNNCTCC	6 cut(s)	428, 458, 667, 697, 1124, 1154
Bse118I	RCCGGY	5 cut(s)	274, 1202, 1577, 1581, 1887
Bse1I	ACTGG	3 cut(s)	76, 1536, 1957
Bse3DI	GCAATG	1 cut(s)	371
BseBI	CCWGG	5 cut(s)	652, 790, 851, 1075, 1253
BseDI	CCNNGG	7 cut(s)	850, 1252, 1383, 1587, 1872, 2023, 2106
BseGI	GGATG	4 cut(s)	248, 1197, 1419, 1833
BseMI	GCAATG	1 cut(s)	371
BseMII	CTCAG	4 cut(s)	730, 1537, 1926, 1995
BseNI	ACTGG	3 cut(s)	76, 1536, 1957
BsePI	GCGCGC	1 cut(s)	2116
BseRI	GAGGAG	8 cut(s)	433, 464, 675, 839, 1369, 1766, 1994, 2093
BseSI	GKGCMC	1 cut(s)	1774
BseX3I	CGGCCG	3 cut(s)	1200, 1204, 1579
BseYI	CCCAGC	1 cut(s)	270
BsgI	GTGCAG	2 cut(s)	76, 2003
Bsh1285I	CGRYCG	5 cut(s)	1203, 1207, 1389, 1582, 1805
BshNI	GGYRCC	2 cut(s)	785, 1916
BsiEI	CGRYCG	5 cut(s)	1203, 1207, 1389, 1582, 1805
BsiHKAI	GWGCWC	4 cut(s)	572, 1752, 1948, 1967
BsiHKCI	CYCGRG	5 cut(s)	415, 704, 806, 1866, 1941
BsiWI	CGTACG	1 cut(s)	1714
BslFI	GGGAC	3 cut(s)	964, 1652, 1672
BsmAI	GTCTC	5 cut(s)	41, 283, 412, 714, 1725
BsmFI	GGGAC	3 cut(s)	964, 1652, 1672
Bso31I	GGTCTC	3 cut(s)	283, 714, 1725
BsoBI	CYCGRG	5 cut(s)	415, 704, 806, 1866, 1941
Bsp119I	TTCGAA	1 cut(s)	2190
Bsp120I	GGGCCC	1 cut(s)	1770
Bsp1286I	GDGCHC	5 cut(s)	572, 1752, 1774, 1948, 1967
Bsp143I	GATC	7 cut(s)	431, 1107, 1211, 1554, 1598, 1608, 1802
Bsp1720I	GCTNAGC	3 cut(s)	1490, 1523, 2004
Bsp68I	TCGCGA	1 cut(s)	1015
BspCNI	CTCAG	4 cut(s)	729, 1536, 1925, 1996
BspLI	GGNNCC	8 cut(s)	787, 1319, 1600, 1687, 1688, 1772, 1918, 2091
BspMAI	CTGCAG	1 cut(s)	302
BspOI	GCTAGC	1 cut(s)	1332
BspPI	GGATC	4 cut(s)	426, 1593, 1603, 1606
BspQI	GCTCTTC	2 cut(s)	11, 1632
BspT104I	TTCGAA	1 cut(s)	2190
BspT107I	GGYRCC	2 cut(s)	785, 1916
BspTNI	GGTCTC	3 cut(s)	283, 714, 1725
BsrBI	CCGCTC	2 cut(s)	766, 1768
BsrDI	GCAATG	1 cut(s)	371
BsrFI	RCCGGY	5 cut(s)	274, 1202, 1577, 1581, 1887
BsrI	ACTGG	3 cut(s)	76, 1536, 1957
BssAI	RCCGGY	5 cut(s)	274, 1202, 1577, 1581, 1887
BssECI	CCNNGG	7 cut(s)	850, 1252, 1383, 1587, 1872, 2023, 2106
BssHII	GCGCGC	1 cut(s)	2116
BssMI	GATC	7 cut(s)	431, 1107, 1211, 1554, 1598, 1608, 1802
BssNI	GRCGYC	8 cut(s)	786, 1007, 1050, 1128, 1196, 1509, 1917, 2099
Bst2UI	CCWGG	5 cut(s)	652, 790, 851, 1075, 1253
Bst4CI	ACNGT	5 cut(s)	423, 1042, 1435, 1719, 2024
Bst6I	CTCTTC	4 cut(s)	11, 896, 1632, 1822
BstACI	GRCGYC	8 cut(s)	786, 1007, 1050, 1128, 1196, 1509, 1917, 2099
BstBAI	YACGTR	1 cut(s)	2074
BstBI	TTCGAA	1 cut(s)	2190
BstC8I	GCNNGC	9 cut(s)	521, 1204, 1330, 1583, 1770, 1790, 1794, 2010, 2118
BstDEI	CTNAG	8 cut(s)	699, 716, 1470, 1490, 1523, 1912, 2004, 2136
BstDSI	CCRYGG	3 cut(s)	1587, 1872, 2023
BstENI	CCTNNNNNAGG	1 cut(s)	1168
BstF5I	GGATG	4 cut(s)	248, 1197, 1419, 1833
BstH2I	RGCGCY	5 cut(s)	789, 947, 1059, 1378, 1920
BstKTI	GATC	7 cut(s)	434, 1110, 1214, 1557, 1601, 1611, 1805
BstMAI	GTCTC	5 cut(s)	41, 283, 412, 714, 1725
BstMBI	GATC	7 cut(s)	431, 1107, 1211, 1554, 1598, 1608, 1802
BstMCI	CGRYCG	5 cut(s)	1203, 1207, 1389, 1582, 1805
BstNI	CCWGG	5 cut(s)	652, 790, 851, 1075, 1253
BstPAI	GACNNNNGTC	1 cut(s)	1043
BstSCI	CCNGG	8 cut(s)	650, 788, 849, 1073, 1251, 1308, 1383, 1668
BstSFI	CTRYAG	3 cut(s)	298, 409, 1726
BstSLI	GKGCMC	1 cut(s)	1774
BstV2I	GAAGAC	1 cut(s)	1800
BstX2I	RGATCY	1 cut(s)	1598
BstYI	RGATCY	1 cut(s)	1598
BstZI	CGGCCG	3 cut(s)	1200, 1204, 1579
BtgI	CCRYGG	3 cut(s)	1587, 1872, 2023
BtgZI	GCGATG	1 cut(s)	1718
BtrI	CACGTC	1 cut(s)	1858
BtsCI	GGATG	4 cut(s)	248, 1197, 1419, 1833
BtuMI	TCGCGA	1 cut(s)	1015
Cac8I	GCNNGC	9 cut(s)	521, 1204, 1330, 1583, 1770, 1790, 1794, 2010, 2118
Cfr10I	RCCGGY	5 cut(s)	274, 1202, 1577, 1581, 1887
Cfr13I	GGNCC	7 cut(s)	84, 1584, 1686, 1719, 1770, 1771, 2047
Csp6I	GTAC	3 cut(s)	546, 1715, 1781
CviAII	CATG	4 cut(s)	605, 625, 769, 2161
CviQI	GTAC	3 cut(s)	546, 1715, 1781
DdeI	CTNAG	8 cut(s)	699, 716, 1470, 1490, 1523, 1912, 2004, 2136
DinI	GGCGCC	2 cut(s)	787, 1918
DpnI	GATC	7 cut(s)	433, 1109, 1213, 1556, 1600, 1610, 1804
DpnII	GATC	7 cut(s)	431, 1107, 1211, 1554, 1598, 1608, 1802
DrdI	GACNNNNNNGTC	1 cut(s)	1424
DriI	GACNNNNNGTC	1 cut(s)	1738
DseDI	GACNNNNNNGTC	1 cut(s)	1424
EaeI	YGGCCR	6 cut(s)	549, 1200, 1204, 1249, 1579, 1875
EagI	CGGCCG	3 cut(s)	1200, 1204, 1579
Eam1104I	CTCTTC	4 cut(s)	11, 896, 1632, 1822
Eam1105I	GACNNNNNGTC	1 cut(s)	1738
EarI	CTCTTC	4 cut(s)	11, 896, 1632, 1822
EciI	GGCGGA	3 cut(s)	479, 1351, 2027
Ecl136II	GAGCTC	3 cut(s)	1750, 1946, 1965
EclXI	CGGCCG	3 cut(s)	1200, 1204, 1579
Eco24I	GRGCYC	4 cut(s)	1752, 1774, 1948, 1967
Eco31I	GGTCTC	3 cut(s)	283, 714, 1725
Eco47I	GGWCC	2 cut(s)	1686, 1719
Eco47III	AGCGCT	1 cut(s)	1376
Eco52I	CGGCCG	3 cut(s)	1200, 1204, 1579
Eco53kI	GAGCTC	3 cut(s)	1750, 1946, 1965
Eco72I	CACGTG	1 cut(s)	2074
Eco88I	CYCGRG	5 cut(s)	415, 704, 806, 1866, 1941
EcoICRI	GAGCTC	3 cut(s)	1750, 1946, 1965
EcoNI	CCTNNNNNAGG	1 cut(s)	1168
EcoO109I	RGGNCCY	3 cut(s)	84, 1686, 1771
EcoRI	GAATTC	1 cut(s)	334
EcoRII	CCWGG	5 cut(s)	650, 788, 849, 1073, 1251
EcoT38I	GRGCYC	4 cut(s)	1752, 1774, 1948, 1967
EgeI	GGCGCC	2 cut(s)	787, 1918
EheI	GGCGCC	2 cut(s)	787, 1918
FaeI	CATG	4 cut(s)	608, 628, 772, 2164
FaiI	YATR	7 cut(s)	606, 626, 770, 1259, 1337, 2147, 2162
FaqI	GGGAC	3 cut(s)	964, 1652, 1672
FatI	CATG	4 cut(s)	604, 624, 768, 2160
FauI	CCCGC	2 cut(s)	1676, 1761
FblI	GTMKAC	2 cut(s)	1047, 1230
FokI	GGATG	4 cut(s)	235, 1204, 1406, 1820
FriOI	GRGCYC	4 cut(s)	1752, 1774, 1948, 1967
FseI	GGCCGGCC	2 cut(s)	1206, 1585
FspBI	CTAG	1 cut(s)	1329
GsaI	CCCAGC	1 cut(s)	274
GsuI	CTGGAG	2 cut(s)	1502, 1940
HaeII	RGCGCY	5 cut(s)	789, 947, 1059, 1378, 1920
Hin1I	GRCGYC	8 cut(s)	786, 1007, 1050, 1128, 1196, 1509, 1917, 2099
Hin1II	CATG	4 cut(s)	608, 628, 772, 2164
HincII	GTYRAC	3 cut(s)	1048, 1231, 1267
HindII	GTYRAC	3 cut(s)	1048, 1231, 1267
HphI	GGTGA	6 cut(s)	79, 202, 397, 1048, 1513, 1567
Hpy166II	GTNNAC	7 cut(s)	748, 1048, 1161, 1231, 1267, 1429, 1722
Hpy188III	TCNNGA	8 cut(s)	280, 610, 727, 806, 869, 967, 1014, 1279
Hpy8I	GTNNAC	7 cut(s)	748, 1048, 1161, 1231, 1267, 1429, 1722
HpyCH4III	ACNGT	5 cut(s)	423, 1042, 1435, 1719, 2024
HpyCH4IV	ACGT	7 cut(s)	372, 1007, 1050, 1196, 1509, 1857, 2073
HpyCH4V	TGCA	7 cut(s)	93, 300, 364, 369, 642, 1300, 2020
HpyF3I	CTNAG	8 cut(s)	699, 716, 1470, 1490, 1523, 1912, 2004, 2136
HpySE526I	ACGT	7 cut(s)	372, 1007, 1050, 1196, 1509, 1857, 2073
Hsp92I	GRCGYC	8 cut(s)	786, 1007, 1050, 1128, 1196, 1509, 1917, 2099
Hsp92II	CATG	4 cut(s)	608, 628, 772, 2164
KasI	GGCGCC	2 cut(s)	785, 1916
KflI	GGGWCCC	1 cut(s)	1686
KroI	GCCGGC	2 cut(s)	1202, 1581
KroNI	GCCGGC	2 cut(s)	1204, 1583
Kzo9I	GATC	7 cut(s)	431, 1107, 1211, 1554, 1598, 1608, 1802
LguI	GCTCTTC	2 cut(s)	11, 1632
LmnI	GCTCC	9 cut(s)	525, 575, 985, 1382, 1521, 1897, 1959, 2014, 2089
LweI	GCATC	1 cut(s)	497
MaeI	CTAG	1 cut(s)	1329
MaeII	ACGT	7 cut(s)	372, 1007, 1050, 1196, 1509, 1857, 2073
MaeIII	GTNAC	7 cut(s)	67, 127, 921, 1036, 1182, 1531, 1858
MalI	GATC	7 cut(s)	433, 1109, 1213, 1556, 1600, 1610, 1804
MbiI	CCGCTC	2 cut(s)	766, 1768
MboI	GATC	7 cut(s)	431, 1107, 1211, 1554, 1598, 1608, 1802
MboII	GAAGA	9 cut(s)	220, 228, 330, 913, 1107, 1649, 1800, 1803, 1809
MflI	RGATCY	1 cut(s)	1598
MhlI	GDGCHC	5 cut(s)	572, 1752, 1774, 1948, 1967
MlsI	TGGCCA	1 cut(s)	1251
MluCI	AATT	3 cut(s)	218, 334, 1675
MluI	ACGCGT	1 cut(s)	1461
MluNI	TGGCCA	1 cut(s)	1251
Mly113I	GGCGCC	2 cut(s)	786, 1917
MlyI	GAGTC	6 cut(s)	277, 716, 818, 946, 1889, 2178
MmeI	TCCRAC	7 cut(s)	459, 837, 1149, 1202, 1417, 1848, 1861
Mox20I	TGGCCA	1 cut(s)	1251
MroNI	GCCGGC	2 cut(s)	1202, 1581
MscI	TGGCCA	1 cut(s)	1251
MseI	TTAA	1 cut(s)	1442
MslI	CAYNNNNRTG	1 cut(s)	2040
Msp20I	TGGCCA	1 cut(s)	1251
MspA1I	CMGCKG	2 cut(s)	297, 2095
MspR9I	CCNGG	8 cut(s)	652, 790, 851, 1075, 1253, 1310, 1385, 1670
MvaI	CCWGG	5 cut(s)	652, 790, 851, 1075, 1253
NaeI	GCCGGC	2 cut(s)	1204, 1583
NarI	GGCGCC	2 cut(s)	786, 1917
NciI	CCSGG	3 cut(s)	1310, 1385, 1670
NdeII	GATC	7 cut(s)	431, 1107, 1211, 1554, 1598, 1608, 1802
NgoMIV	GCCGGC	2 cut(s)	1202, 1581
NheI	GCTAGC	1 cut(s)	1328
NlaIII	CATG	4 cut(s)	608, 628, 772, 2164
NlaIV	GGNNCC	8 cut(s)	787, 1319, 1600, 1687, 1688, 1772, 1918, 2091
NmeAIII	GCCGAG	7 cut(s)	589, 1576, 1596, 1903, 1946, 2087, 2149
NmuCI	GTSAC	3 cut(s)	67, 1036, 1858
NruI	TCGCGA	1 cut(s)	1015
NspV	TTCGAA	1 cut(s)	2190
OliI	CACNNNNGTG	1 cut(s)	2040
PaeR7I	CTCGAG	2 cut(s)	1866, 1941
PalAI	GGCGCGCC	1 cut(s)	2116
PauI	GCGCGC	1 cut(s)	2116
PciSI	GCTCTTC	2 cut(s)	11, 1632
PcsI	WCGNNNNNNNCGW	3 cut(s)	1422, 1443, 1473
PdiI	GCCGGC	2 cut(s)	1204, 1583
PfeI	GAWTC	4 cut(s)	55, 513, 929, 1078
Pfl23II	CGTACG	1 cut(s)	1714
PflFI	GACNNNGTC	1 cut(s)	1009
PflMI	CCANNNNNTGG	1 cut(s)	2044
Ple19I	CGATCG	1 cut(s)	1805
PleI	GAGTC	6 cut(s)	277, 715, 817, 946, 1888, 2177
PluTI	GGCGCC	2 cut(s)	789, 1920
PmaCI	CACGTG	1 cut(s)	2074
PmlI	CACGTG	1 cut(s)	2074
PpsI	GAGTC	6 cut(s)	277, 715, 817, 946, 1888, 2177
Ppu21I	YACGTR	1 cut(s)	2074
PpuMI	RGGWCCY	1 cut(s)	1686
PshAI	GACNNNNGTC	1 cut(s)	1043
Psp124BI	GAGCTC	3 cut(s)	1752, 1948, 1967
Psp5II	RGGWCCY	1 cut(s)	1686
Psp6I	CCWGG	5 cut(s)	650, 788, 849, 1073, 1251
PspCI	CACGTG	1 cut(s)	2074
PspFI	CCCAGC	1 cut(s)	270
PspGI	CCWGG	5 cut(s)	650, 788, 849, 1073, 1251
PspLI	CGTACG	1 cut(s)	1714
PspN4I	GGNNCC	8 cut(s)	787, 1319, 1600, 1687, 1688, 1772, 1918, 2091
PspOMI	GGGCCC	1 cut(s)	1770
PspPI	GGNCC	7 cut(s)	84, 1584, 1686, 1719, 1770, 1771, 2047
PspPPI	RGGWCCY	1 cut(s)	1686
PspXI	VCTCGAGB	2 cut(s)	1866, 1941
PstI	CTGCAG	1 cut(s)	302
PsuI	RGATCY	1 cut(s)	1598
PsyI	GACNNNGTC	1 cut(s)	1009
PteI	GCGCGC	1 cut(s)	2116
PvuI	CGATCG	1 cut(s)	1805
RigI	GGCCGGCC	2 cut(s)	1206, 1585
RruI	TCGCGA	1 cut(s)	1015
RsaI	GTAC	3 cut(s)	547, 1716, 1782
RsaNI	GTAC	3 cut(s)	546, 1715, 1781
RseI	CAYNNNNRTG	1 cut(s)	2040
SacI	GAGCTC	3 cut(s)	1752, 1948, 1967
SalI	GTCGAC	2 cut(s)	1046, 1229
SapI	GCTCTTC	2 cut(s)	11, 1632
SaqAI	TTAA	1 cut(s)	1442
Sau3AI	GATC	7 cut(s)	431, 1107, 1211, 1554, 1598, 1608, 1802
Sau96I	GGNCC	7 cut(s)	84, 1584, 1686, 1719, 1770, 1771, 2047
SchI	GAGTC	6 cut(s)	277, 716, 818, 946, 1889, 2178
ScrFI	CCNGG	8 cut(s)	652, 790, 851, 1075, 1253, 1310, 1385, 1670
SduI	GDGCHC	5 cut(s)	572, 1752, 1774, 1948, 1967
SfaNI	GCATC	1 cut(s)	497
SfcI	CTRYAG	3 cut(s)	298, 409, 1726
SfoI	GGCGCC	2 cut(s)	787, 1918
Sfr274I	CTCGAG	2 cut(s)	1866, 1941
SfuI	TTCGAA	1 cut(s)	2190
SgsI	GGCGCGCC	1 cut(s)	2116
SinI	GGWCC	2 cut(s)	1686, 1719
SlaI	CTCGAG	2 cut(s)	1866, 1941
SmiMI	CAYNNNNRTG	1 cut(s)	2040
SmlI	CTYRAG	5 cut(s)	725, 739, 1277, 1866, 1941
SmoI	CTYRAG	5 cut(s)	725, 739, 1277, 1866, 1941
Sse9I	AATT	3 cut(s)	218, 334, 1675
SspDI	GGCGCC	2 cut(s)	785, 1916
SspMI	CTAG	1 cut(s)	1329
SstI	GAGCTC	3 cut(s)	1752, 1948, 1967
StyD4I	CCNGG	8 cut(s)	650, 788, 849, 1073, 1251, 1308, 1383, 1668
TaaI	ACNGT	5 cut(s)	423, 1042, 1435, 1719, 2024
TaiI	ACGT	7 cut(s)	375, 1010, 1053, 1199, 1512, 1860, 2076
TasI	AATT	3 cut(s)	218, 334, 1675
TfiI	GAWTC	4 cut(s)	55, 513, 929, 1078
Tru1I	TTAA	1 cut(s)	1442
Tru9I	TTAA	1 cut(s)	1442
TseFI	GTSAC	3 cut(s)	67, 1036, 1858
Tsp45I	GTSAC	3 cut(s)	67, 1036, 1858
TspDTI	ATGAA	1 cut(s)	2177
TspGWI	ACGGA	3 cut(s)	1201, 1702, 2082
Tth111I	GACNNNGTC	1 cut(s)	1009
Van91I	CCANNNNNTGG	1 cut(s)	2044
VpaK11BI	GGWCC	2 cut(s)	1686, 1719
XagI	CCTNNNNNAGG	1 cut(s)	1168
XapI	RAATTY	2 cut(s)	218, 334
XhoI	CTCGAG	2 cut(s)	1866, 1941
XmiI	GTMKAC	2 cut(s)	1047, 1230
XspI	CTAG	1 cut(s)	1329
ZraI	GACGTC	4 cut(s)	1008, 1051, 1197, 1510

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.