Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0011222

Physical Location & Seq

Genomic Coordinates Reverse (-)

21889652 .. 21893238

Gene Structure 3587 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG225400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2148 bp

ATGGTGACTGGGTGGAGAAGGGCCTTCTGCACGTCTATCCCTAAAGACAGACACACCAAAGTTGTAACAGAGAAGCAGCAGCAACAATGTGAAAACACCAACACCAACACCAACAATAACCAAAGCCCCAAAATCACCTCCAAATTCGGTTTCTTCTCCTCTTCCTCAAACCCATCCACGCCTCGCCTTCAATCCCAGCCGGTGTCGACTCCAAGCCTCCGCTGCCGAACCACCACAGCCGCCGCCACAGCACCCACCACCCCCAATTCTTCCCTCCCCAACAGCCCAAAACTGCAATGCAACGTCCCTGCCACCCCGAAAAAAATGACCTACAGCCCCGGAGTGTTCCACCGATCCAACCCCTCCTCCCCGAAATCGCCTTCCAGCTTCTCCCTCCTCAAATCCACCCTCCGCCTCAACAAAAGTAGATGCGGAATCTGCTTGCAGAGCGTGAAAAGTGGTCAAGGTACGGCCATTTTCACAGCCGAGTGCTCCCACTCCTTTCACTTTCCTTGCGTCGCCACCCATGTCAAGCAGCAACCTCTCATGCTCTGCCCCGTCTGCCACGCCACCTGGAAGGAGCTGCCTCTGCTCGAAACTCTCACTCCTCCTCCTCGCACCAATACCAAGCTCAGAGACGTCAAGATCAAGCCCCTGAGGGTCTACAACGACGACGAGCCCCTCATGTCGCCCACCTCCGGCGCCAGGTTCAATCCCATTCCGGAGTCCGACGAAAACGAGGACGAAAACGACGCCGTTGAGTTCCAGGGCTTCTTTGTCAATCCTGATGCGAATAATGTCTCTTCCGGGAATCGCAAGAGTGTGGAGTTTAGTTTGCTGCCTGAATCGGCGGTGGTGGCGCTTGGGAGGAGCTTCGAGACTTACGCCGTCGTCTTGAAAGTCAAAGCTCCGCAGCTTGTGGAAGGCAGAGCGACGTCGTCGTCGCGAAGGGCGCCGATTGACTTGGTGACCGTGGTCGACGTCAGCGCCAATACCAGCCGCGCCAGGATTCAGGCCACCAAACGCGCGTTGCGGTTGATCATTTCTTCTCTCTCCGACGCCGACAGGCTCTCCATCGTGGCCTTCTCGTCCACCTCCAAGAGGCTGCTGCCGTTACGGAGGATGACGTCGCCCGGCCGGAGATCGGCGCGTCGGATAGTCGACGCGCTCTGCGGCGTTGGCCAGGGTATGTGTGTCAACGACGCGCTCAAGAAGGCGGCCAAGGTGTTGCAAGACCGCCGGGAGAGGAACCCCGTCGCTAGCATTATGCTCCTCTCGGAAGGCGGCGGCGGCGGCGTTTCCGCCAATCAGAAGCGCTCCTCCCCGGTCGTGTCCTCCACGCGCTTCCCCCACCTCGACATCCCCGTCCACACCGTCGGATTAGCCGAGACTACCGACGACGCGTTCGCCAAGTGCGTGGGGGGTTTGCTAAGCGTGGTGGTGAAAGACGTCAAGCTCCAGCTCAGCGTCGTAACCGGTTCAGCCAACGCCGAGATCGCGGCGGTTTATTCTCTCACGGGCCGGCCCGCGGCTCTCGGATCCGGTTCGATCCGGCTCGGCGACCTCTACGCCGGAGAAGAGCGAGAGCTGCTCGTCGAGTTGAAAGTCCCGGTCAATTCCGGCGGGTCCCACACCCAAAACGCGATGATGTCCGTACGGTCCACCTACAGAGACCCGTCGTCGAATGAGCTCGTATTTTCCAAAGAGCGGGCCCTCCTCGTACCGCGCTCGCAAGCCGTCCGATCGCCTTCTACCTCTTCCTCCAACCCCAACATCCAACGGCTGCGAAATCTCCACGTCACGGCTCGAGCCGTGGCCGAATCGCGCCGGTTCTTGGAGCGAAACGACTTCTCAGGCGCCCATCACTTGCTGACTTCGGCTCGAGCTCTGCTGCTCCAGTCGAGCTCGGCTTCGGCGGAGGAGTTCCTGCGCGGCTTGGAGGCTGAGCTGGCGGAGCTGCACCGTGGAAGGCAGCACCAGAGTGTGGCCCAGAGGCAGAGAGGGAACGGGCACGTGGAGGAGAAGCCGGAACCGCTGACGCCGACCTCGGCTTGGCGCGCCGCCGAGAGATTGGCTAAGGTGGCTATAATGAGGAAGTCCATGAACAGAGTCAGCGACTTACACGGCTTCGAAAACGCCAGATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000741 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006807 GO:0006996 GO:0006997 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009553 GO:0009559 GO:0009561 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009987 GO:0010197 GO:0016020 GO:0016043 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022622 GO:0030054 GO:0030154 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048229 GO:0048284 GO:0048364 GO:0048731 GO:0048856 GO:0048869 GO:0050896 GO:0055044 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0099402 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

VWA VWA_2 VWA_3 zf-RING_11

Protein Analysis

715

Amino Acids

77.75

Weight (kDa)

10.02

Isoelectric Point (pI)

53.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	143 - 188	4.6e-07	Ring finger domain
zf-RING_11	PF17123	144 - 173	1.5e-08	RING-like zinc finger
VWA	PF00092	321 - 467	8.7e-11	von Willebrand factor type A domain
VWA_3	PF13768	321 - 467	4.5e-10	von Willebrand factor type A domain
VWA_2	PF13519	322 - 428	2.5e-14	von Willebrand factor type A domain
WAV3_C	PF25243	590 - 663	4.7e-35	E3 ubiquitin-protein ligase WAV3-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22680 AT4G37890 AT4G37890
fragaria_vesca	FvH4_1g18891
malus_domestica	MD01G1045900.v1.1 MD15G1305700.v1.1
prunus_persica	Prupe.6G210600_v2.0.a1
pyrus_communis	pycom01g07210 pycom15g26960
rosa_chinensis	RchiOBHm_Chr2g0109851
rosa_laevigata	RLG00000017767
rosa_multiflora	Rmu_co8072298.1_g000001 Rmu_co8290675.1_g000001 Rmu_sc0008299.1_g000001
rosa_roxburghii	Rroxscaffold_2G00133910
rosa_samantha	Rh2AG215300 Rh2BG225400 Rh2CG217200 Rh2DG220800
rosa_wichuraiana	Rw2G016630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	940, 1364
AatII	GACGTC	5 cut(s)	642, 938, 984, 1130, 1452
AccB1I	GGYRCC	3 cut(s)	701, 952, 1856
AccB7I	CCANNNNNTGG	1 cut(s)	1984
AccBSI	CCGCTC	1 cut(s)	1708
AccI	GTMKAC	4 cut(s)	206, 663, 978, 1161
AccIII	TCCGGA	1 cut(s)	721
AclWI	GGATC	4 cut(s)	348, 1533, 1543, 1546
AcoI	YGGCCR	5 cut(s)	471, 1135, 1180, 1218, 1815
AcsI	RAATTY	1 cut(s)	143
AcvI	CACGTG	1 cut(s)	2014
AfaI	GTAC	3 cut(s)	469, 1656, 1722
AfeI	AGCGCT	1 cut(s)	1316
AflIII	ACRYGT	1 cut(s)	1401
AgeI	ACCGGT	1 cut(s)	1475
AgsI	TTSAA	4 cut(s)	191, 712, 898, 1603
AhdI	GACNNNNNGTC	1 cut(s)	1678
AjiI	CACGTC	2 cut(s)	33, 1798
AjnI	CCWGG	5 cut(s)	572, 704, 765, 1004, 1182
AjuI	GAANNNNNNNTTGG	2 cut(s)	134, 166
AleI	CACNNNNGTG	1 cut(s)	1980
Alw21I	GWGCWC	4 cut(s)	494, 1692, 1888, 1907
Alw26I	GTCTC	5 cut(s)	630, 805, 872, 1382, 1665
AlwI	GGATC	4 cut(s)	348, 1533, 1543, 1546
Ama87I	CYCGRG	2 cut(s)	1806, 1881
Aor13HI	TCCGGA	1 cut(s)	721
Aor51HI	AGCGCT	1 cut(s)	1316
ApaI	GGGCCC	1 cut(s)	1714
ApoI	RAATTY	1 cut(s)	143
ArsI	GACNNNNNNTTYG	2 cut(s)	1388, 1420
AscI	GGCGCGCC	1 cut(s)	2056
AsiGI	ACCGGT	1 cut(s)	1475
AspS9I	GGNCC	8 cut(s)	21, 1519, 1524, 1626, 1659, 1710, 1711, 1987
AsuC2I	CCSGG	6 cut(s)	339, 808, 1134, 1241, 1325, 1610
AsuHPI	GGTGA	4 cut(s)	16, 127, 979, 1453
AsuII	TTCGAA	1 cut(s)	2130
AsuNHI	GCTAGC	1 cut(s)	1259
AvaI	CYCGRG	2 cut(s)	1806, 1881
AvaII	GGWCC	2 cut(s)	1626, 1659
AxyI	CCTNAGG	1 cut(s)	656
BaeGI	GKGCMC	2 cut(s)	1714, 2013
BalI	TGGCCA	1 cut(s)	1182
BamHI	GGATCC	1 cut(s)	1538
BanI	GGYRCC	3 cut(s)	701, 952, 1856
BanII	GRGCYC	5 cut(s)	681, 1692, 1714, 1888, 1907
BbrPI	CACGTG	1 cut(s)	2014
Bbv12I	GWGCWC	4 cut(s)	494, 1692, 1888, 1907
BccI	CCATC	3 cut(s)	181, 1082, 1869
BceAI	ACGGC	9 cut(s)	486, 740, 872, 1096, 1721, 1796, 1796, 1818, 2140
BciT130I	CCWGG	5 cut(s)	574, 706, 767, 1006, 1184
BclI	TGATCA	1 cut(s)	1038
BcnI	CCSGG	6 cut(s)	339, 808, 1134, 1241, 1325, 1610
BcoDI	GTCTC	5 cut(s)	630, 805, 872, 1382, 1665
BfaI	CTAG	1 cut(s)	1260
BfmI	CTRYAG	2 cut(s)	331, 1666
BfoI	RGCGCY	6 cut(s)	705, 863, 956, 990, 1318, 1860
BlpI	GCTNAGC	3 cut(s)	1430, 1463, 1944
Bme18I	GGWCC	2 cut(s)	1626, 1659
BmeRI	GACNNNNNGTC	1 cut(s)	1678
BmeT110I	CYCGRG	2 cut(s)	1806, 1881
BmgBI	CACGTC	2 cut(s)	33, 1798
BmgT120I	GGNCC	8 cut(s)	21, 1519, 1524, 1626, 1659, 1710, 1711, 1987
BmiI	GGNNCC	9 cut(s)	703, 954, 1250, 1540, 1627, 1628, 1712, 1858, 2031
BmrI	ACTGGG	1 cut(s)	18
BmsI	GCATC	2 cut(s)	419, 778
BmtI	GCTAGC	1 cut(s)	1263
BmuI	ACTGGG	1 cut(s)	18
BoxI	GACNNNNGTC	1 cut(s)	974
BplI	GAGNNNNNCTC	2 cut(s)	1575, 1607
BpmI	CTGGAG	2 cut(s)	1442, 1880
Bpu10I	CCTNAGC	1 cut(s)	2076
Bpu1102I	GCTNAGC	3 cut(s)	1430, 1463, 1944
Bpu14I	TTCGAA	1 cut(s)	2130
BpuEI	CTTGAG	1 cut(s)	1193
BpuMI	CCSGG	6 cut(s)	339, 808, 1134, 1241, 1325, 1610
BsaAI	YACGTR	1 cut(s)	2014
BsaI	GGTCTC	1 cut(s)	1665
BsaWI	WCCGGW	3 cut(s)	721, 1475, 1541
BsaXI	ACNNNNNCTCC	8 cut(s)	350, 380, 589, 595, 619, 625, 1055, 1085
Bse118I	RCCGGY	4 cut(s)	199, 1475, 1521, 1827
Bse1I	ACTGG	2 cut(s)	13, 1897
Bse21I	CCTNAGG	1 cut(s)	656
Bse3DI	GCAATG	1 cut(s)	302
BseAI	TCCGGA	1 cut(s)	721
BseBI	CCWGG	5 cut(s)	574, 706, 767, 1006, 1184
BseGI	GGATG	4 cut(s)	173, 1128, 1359, 1773
BseMI	GCAATG	1 cut(s)	302
BseMII	CTCAG	5 cut(s)	646, 647, 1477, 1866, 1935
BseNI	ACTGG	2 cut(s)	13, 1897
BsePI	GCGCGC	1 cut(s)	2056
BseSI	GKGCMC	2 cut(s)	1714, 2013
BseX3I	CGGCCG	1 cut(s)	1135
BseYI	CCCAGC	1 cut(s)	195
BsgI	GTGCAG	2 cut(s)	13, 1943
Bsh1285I	CGRYCG	3 cut(s)	1138, 1329, 1745
BshNI	GGYRCC	3 cut(s)	701, 952, 1856
BshTI	ACCGGT	1 cut(s)	1475
BsiEI	CGRYCG	3 cut(s)	1138, 1329, 1745
BsiHKAI	GWGCWC	4 cut(s)	494, 1692, 1888, 1907
BsiHKCI	CYCGRG	2 cut(s)	1806, 1881
BsiWI	CGTACG	1 cut(s)	1654
BslFI	GGGAC	3 cut(s)	290, 1592, 1612
BsmAI	GTCTC	5 cut(s)	630, 805, 872, 1382, 1665
BsmBI	CGTCTC	1 cut(s)	630
BsmFI	GGGAC	3 cut(s)	290, 1592, 1612
Bso31I	GGTCTC	1 cut(s)	1665
BsoBI	CYCGRG	2 cut(s)	1806, 1881
Bsp119I	TTCGAA	1 cut(s)	2130
Bsp120I	GGGCCC	1 cut(s)	1710
Bsp1286I	GDGCHC	7 cut(s)	494, 681, 1692, 1714, 1888, 1907, 2013
Bsp13I	TCCGGA	1 cut(s)	721
Bsp143I	GATC	8 cut(s)	353, 645, 1038, 1142, 1494, 1538, 1548, 1742
Bsp1720I	GCTNAGC	3 cut(s)	1430, 1463, 1944
Bsp68I	TCGCGA	1 cut(s)	946
BspCNI	CTCAG	5 cut(s)	645, 648, 1476, 1865, 1936
BspEI	TCCGGA	1 cut(s)	721
BspLI	GGNNCC	9 cut(s)	703, 954, 1250, 1540, 1627, 1628, 1712, 1858, 2031
BspOI	GCTAGC	1 cut(s)	1263
BspPI	GGATC	4 cut(s)	348, 1533, 1543, 1546
BspQI	GCTCTTC	1 cut(s)	1572
BspT104I	TTCGAA	1 cut(s)	2130
BspT107I	GGYRCC	3 cut(s)	701, 952, 1856
BspTNI	GGTCTC	1 cut(s)	1665
BsrBI	CCGCTC	1 cut(s)	1708
BsrDI	GCAATG	1 cut(s)	302
BsrFI	RCCGGY	4 cut(s)	199, 1475, 1521, 1827
BsrI	ACTGG	2 cut(s)	13, 1897
BssAI	RCCGGY	4 cut(s)	199, 1475, 1521, 1827
BssHII	GCGCGC	1 cut(s)	2056
BssMI	GATC	8 cut(s)	353, 645, 1038, 1142, 1494, 1538, 1548, 1742
BssT1I	CCWWGG	1 cut(s)	1221
Bst2UI	CCWGG	5 cut(s)	574, 706, 767, 1006, 1184
Bst4CI	ACNGT	4 cut(s)	973, 1375, 1659, 1964
Bst6I	CTCTTC	4 cut(s)	166, 808, 1572, 1762
BstBAI	YACGTR	1 cut(s)	2014
BstBI	TTCGAA	1 cut(s)	2130
BstC8I	GCNNGC	9 cut(s)	443, 1261, 1523, 1527, 1710, 1730, 1734, 1950, 2058
BstDEI	CTNAG	7 cut(s)	632, 656, 1430, 1463, 1852, 1944, 2076
BstDSI	CCRYGG	4 cut(s)	972, 1527, 1812, 1963
BstEII	GGTNACC	1 cut(s)	967
BstENI	CCTNNNNNAGG	1 cut(s)	1099
BstF5I	GGATG	4 cut(s)	173, 1128, 1359, 1773
BstH2I	RGCGCY	6 cut(s)	705, 863, 956, 990, 1318, 1860
BstKTI	GATC	8 cut(s)	356, 648, 1041, 1145, 1497, 1541, 1551, 1745
BstMAI	GTCTC	5 cut(s)	630, 805, 872, 1382, 1665
BstMBI	GATC	8 cut(s)	353, 645, 1038, 1142, 1494, 1538, 1548, 1742
BstMCI	CGRYCG	3 cut(s)	1138, 1329, 1745
BstNI	CCWGG	5 cut(s)	574, 706, 767, 1006, 1184
BstPAI	GACNNNNGTC	1 cut(s)	974
BstPI	GGTNACC	1 cut(s)	967
BstSFI	CTRYAG	2 cut(s)	331, 1666
BstSLI	GKGCMC	2 cut(s)	1714, 2013
BstX2I	RGATCY	1 cut(s)	1538
BstXI	CCANNNNNNTGG	1 cut(s)	1417
BstYI	RGATCY	1 cut(s)	1538
BstZI	CGGCCG	1 cut(s)	1135
Bsu36I	CCTNAGG	1 cut(s)	656
BtgI	CCRYGG	4 cut(s)	972, 1527, 1812, 1963
BtgZI	GCGATG	1 cut(s)	1658
BtrI	CACGTC	2 cut(s)	33, 1798
BtsCI	GGATG	4 cut(s)	173, 1128, 1359, 1773
BtuMI	TCGCGA	1 cut(s)	946
Cac8I	GCNNGC	9 cut(s)	443, 1261, 1523, 1527, 1710, 1730, 1734, 1950, 2058
Cfr10I	RCCGGY	4 cut(s)	199, 1475, 1521, 1827
Cfr13I	GGNCC	8 cut(s)	21, 1519, 1524, 1626, 1659, 1710, 1711, 1987
Cfr42I	CCGCGG	1 cut(s)	1530
CseI	GACGC	9 cut(s)	505, 761, 1067, 1139, 1172, 1211, 1409, 1456, 2047
Csp6I	GTAC	3 cut(s)	468, 1655, 1721
CspAI	ACCGGT	1 cut(s)	1475
CviAII	CATG	4 cut(s)	527, 547, 685, 2101
CviQI	GTAC	3 cut(s)	468, 1655, 1721
DdeI	CTNAG	7 cut(s)	632, 656, 1430, 1463, 1852, 1944, 2076
DinI	GGCGCC	3 cut(s)	703, 954, 1858
DpnI	GATC	8 cut(s)	355, 647, 1040, 1144, 1496, 1540, 1550, 1744
DpnII	GATC	8 cut(s)	353, 645, 1038, 1142, 1494, 1538, 1548, 1742
DrdI	GACNNNNNNGTC	2 cut(s)	940, 1364
DriI	GACNNNNNGTC	1 cut(s)	1678
DseDI	GACNNNNNNGTC	2 cut(s)	940, 1364
EaeI	YGGCCR	5 cut(s)	471, 1135, 1180, 1218, 1815
EagI	CGGCCG	1 cut(s)	1135
Eam1104I	CTCTTC	4 cut(s)	166, 808, 1572, 1762
Eam1105I	GACNNNNNGTC	1 cut(s)	1678
EarI	CTCTTC	4 cut(s)	166, 808, 1572, 1762
EciI	GGCGGA	4 cut(s)	401, 1291, 1931, 1967
Ecl136II	GAGCTC	3 cut(s)	1690, 1886, 1905
EclXI	CGGCCG	1 cut(s)	1135
Eco130I	CCWWGG	1 cut(s)	1221
Eco24I	GRGCYC	5 cut(s)	681, 1692, 1714, 1888, 1907
Eco31I	GGTCTC	1 cut(s)	1665
Eco47I	GGWCC	2 cut(s)	1626, 1659
Eco47III	AGCGCT	1 cut(s)	1316
Eco52I	CGGCCG	1 cut(s)	1135
Eco53kI	GAGCTC	3 cut(s)	1690, 1886, 1905
Eco72I	CACGTG	1 cut(s)	2014
Eco81I	CCTNAGG	1 cut(s)	656
Eco88I	CYCGRG	2 cut(s)	1806, 1881
Eco91I	GGTNACC	1 cut(s)	967
EcoICRI	GAGCTC	3 cut(s)	1690, 1886, 1905
EcoNI	CCTNNNNNAGG	1 cut(s)	1099
EcoO109I	RGGNCCY	3 cut(s)	21, 1626, 1711
EcoO65I	GGTNACC	1 cut(s)	967
EcoRII	CCWGG	5 cut(s)	572, 704, 765, 1004, 1182
EcoT14I	CCWWGG	1 cut(s)	1221
EcoT38I	GRGCYC	5 cut(s)	681, 1692, 1714, 1888, 1907
EgeI	GGCGCC	3 cut(s)	703, 954, 1858
EheI	GGCGCC	3 cut(s)	703, 954, 1858
ErhI	CCWWGG	1 cut(s)	1221
Esp3I	CGTCTC	1 cut(s)	630
FaeI	CATG	4 cut(s)	530, 550, 688, 2104
FaiI	YATR	7 cut(s)	528, 548, 686, 1190, 1268, 2087, 2102
FaqI	GGGAC	3 cut(s)	290, 1592, 1612
FatI	CATG	4 cut(s)	526, 546, 684, 2100
FauI	CCCGC	3 cut(s)	1534, 1616, 1701
FbaI	TGATCA	1 cut(s)	1038
FblI	GTMKAC	4 cut(s)	206, 663, 978, 1161
FokI	GGATG	4 cut(s)	160, 1135, 1346, 1760
FriOI	GRGCYC	5 cut(s)	681, 1692, 1714, 1888, 1907
FseI	GGCCGGCC	1 cut(s)	1525
FspBI	CTAG	1 cut(s)	1260
GsaI	CCCAGC	1 cut(s)	199
GsuI	CTGGAG	2 cut(s)	1442, 1880
HaeII	RGCGCY	6 cut(s)	705, 863, 956, 990, 1318, 1860
HgaI	GACGC	9 cut(s)	505, 761, 1067, 1139, 1172, 1211, 1409, 1456, 2047
Hin1II	CATG	4 cut(s)	530, 550, 688, 2104
HincII	GTYRAC	4 cut(s)	207, 979, 1162, 1198
HindII	GTYRAC	4 cut(s)	207, 979, 1162, 1198
HinfI	GANTC	8 cut(s)	208, 435, 725, 811, 845, 1009, 1820, 2109
HphI	GGTGA	4 cut(s)	16, 127, 979, 1453
Hpy166II	GTNNAC	8 cut(s)	207, 664, 979, 1092, 1162, 1198, 1369, 1662
Hpy188I	TCNGA	9 cut(s)	635, 730, 1057, 1155, 1279, 1311, 1379, 1538, 1742
Hpy188III	TCNNGA	7 cut(s)	643, 722, 785, 877, 895, 945, 1210
Hpy8I	GTNNAC	8 cut(s)	207, 664, 979, 1092, 1162, 1198, 1369, 1662
HpyCH4III	ACNGT	4 cut(s)	973, 1375, 1659, 1964
HpyCH4IV	ACGT	9 cut(s)	32, 303, 639, 935, 981, 1127, 1449, 1797, 2013
HpyCH4V	TGCA	6 cut(s)	30, 295, 300, 445, 1231, 1960
HpyF3I	CTNAG	7 cut(s)	632, 656, 1430, 1463, 1852, 1944, 2076
HpySE526I	ACGT	9 cut(s)	32, 303, 639, 935, 981, 1127, 1449, 1797, 2013
Hsp92II	CATG	4 cut(s)	530, 550, 688, 2104
KasI	GGCGCC	3 cut(s)	701, 952, 1856
KflI	GGGWCCC	1 cut(s)	1626
Kpn2I	TCCGGA	1 cut(s)	721
KroI	GCCGGC	1 cut(s)	1521
KroNI	GCCGGC	1 cut(s)	1523
Ksp22I	TGATCA	1 cut(s)	1038
KspI	CCGCGG	1 cut(s)	1530
Kzo9I	GATC	8 cut(s)	353, 645, 1038, 1142, 1494, 1538, 1548, 1742
LguI	GCTCTTC	1 cut(s)	1572
LweI	GCATC	2 cut(s)	419, 778
MaeI	CTAG	1 cut(s)	1260
MaeII	ACGT	9 cut(s)	32, 303, 639, 935, 981, 1127, 1449, 1797, 2013
MaeIII	GTNAC	6 cut(s)	4, 64, 967, 1113, 1471, 1798
MalI	GATC	8 cut(s)	355, 647, 1040, 1144, 1496, 1540, 1550, 1744
MbiI	CCGCTC	1 cut(s)	1708
MboI	GATC	8 cut(s)	353, 645, 1038, 1142, 1494, 1538, 1548, 1742
MboII	GAAGA	7 cut(s)	145, 153, 261, 795, 1038, 1589, 1749
MflI	RGATCY	1 cut(s)	1538
MhlI	GDGCHC	7 cut(s)	494, 681, 1692, 1714, 1888, 1907, 2013
MlsI	TGGCCA	1 cut(s)	1182
MluCI	AATT	3 cut(s)	143, 265, 1615
MluI	ACGCGT	1 cut(s)	1401
MluNI	TGGCCA	1 cut(s)	1182
Mly113I	GGCGCC	3 cut(s)	702, 953, 1857
MlyI	GAGTC	3 cut(s)	202, 734, 2118
MmeI	TCCRAC	7 cut(s)	381, 753, 1080, 1133, 1357, 1788, 1801
Mox20I	TGGCCA	1 cut(s)	1182
MroI	TCCGGA	1 cut(s)	721
MroNI	GCCGGC	1 cut(s)	1521
MscI	TGGCCA	1 cut(s)	1182
MslI	CAYNNNNRTG	1 cut(s)	1980
Msp20I	TGGCCA	1 cut(s)	1182
MspA1I	CMGCKG	3 cut(s)	222, 1529, 2035
MvaI	CCWGG	5 cut(s)	574, 706, 767, 1006, 1184
NaeI	GCCGGC	1 cut(s)	1523
NarI	GGCGCC	3 cut(s)	702, 953, 1857
NciI	CCSGG	6 cut(s)	339, 808, 1134, 1241, 1325, 1610
NdeII	GATC	8 cut(s)	353, 645, 1038, 1142, 1494, 1538, 1548, 1742
NgoMIV	GCCGGC	1 cut(s)	1521
NheI	GCTAGC	1 cut(s)	1259
NlaIII	CATG	4 cut(s)	530, 550, 688, 2104
NlaIV	GGNNCC	9 cut(s)	703, 954, 1250, 1540, 1627, 1628, 1712, 1858, 2031
NmeAIII	GCCGAG	7 cut(s)	511, 1411, 1516, 1536, 1886, 2027, 2089
NmuCI	GTSAC	3 cut(s)	4, 967, 1798
NruI	TCGCGA	1 cut(s)	946
NspV	TTCGAA	1 cut(s)	2130
OliI	CACNNNNGTG	1 cut(s)	1980
PaeR7I	CTCGAG	2 cut(s)	1806, 1881
PalAI	GGCGCGCC	1 cut(s)	2056
PauI	GCGCGC	1 cut(s)	2056
PciSI	GCTCTTC	1 cut(s)	1572
PcsI	WCGNNNNNNNCGW	3 cut(s)	1362, 1383, 1413
PdiI	GCCGGC	1 cut(s)	1523
PfeI	GAWTC	5 cut(s)	435, 811, 845, 1009, 1820
Pfl23II	CGTACG	1 cut(s)	1654
PflFI	GACNNNGTC	1 cut(s)	937
PflMI	CCANNNNNTGG	1 cut(s)	1984
PfoI	TCCNGGA	1 cut(s)	806
PinAI	ACCGGT	1 cut(s)	1475
Ple19I	CGATCG	1 cut(s)	1745
PleI	GAGTC	3 cut(s)	202, 733, 2117
PluTI	GGCGCC	3 cut(s)	705, 956, 1860
PmaCI	CACGTG	1 cut(s)	2014
PmlI	CACGTG	1 cut(s)	2014
PpsI	GAGTC	3 cut(s)	202, 733, 2117
Ppu21I	YACGTR	1 cut(s)	2014
PpuMI	RGGWCCY	1 cut(s)	1626
PshAI	GACNNNNGTC	1 cut(s)	974
Psp124BI	GAGCTC	3 cut(s)	1692, 1888, 1907
Psp5II	RGGWCCY	1 cut(s)	1626
Psp6I	CCWGG	5 cut(s)	572, 704, 765, 1004, 1182
PspCI	CACGTG	1 cut(s)	2014
PspEI	GGTNACC	1 cut(s)	967
PspFI	CCCAGC	1 cut(s)	195
PspGI	CCWGG	5 cut(s)	572, 704, 765, 1004, 1182
PspLI	CGTACG	1 cut(s)	1654
PspN4I	GGNNCC	9 cut(s)	703, 954, 1250, 1540, 1627, 1628, 1712, 1858, 2031
PspOMI	GGGCCC	1 cut(s)	1710
PspPI	GGNCC	8 cut(s)	21, 1519, 1524, 1626, 1659, 1710, 1711, 1987
PspPPI	RGGWCCY	1 cut(s)	1626
PspXI	VCTCGAGB	2 cut(s)	1806, 1881
PsuI	RGATCY	1 cut(s)	1538
PsyI	GACNNNGTC	1 cut(s)	937
PteI	GCGCGC	1 cut(s)	2056
PvuI	CGATCG	1 cut(s)	1745
RigI	GGCCGGCC	1 cut(s)	1525
RruI	TCGCGA	1 cut(s)	946
RsaI	GTAC	3 cut(s)	469, 1656, 1722
RsaNI	GTAC	3 cut(s)	468, 1655, 1721
RseI	CAYNNNNRTG	1 cut(s)	1980
SacI	GAGCTC	3 cut(s)	1692, 1888, 1907
SacII	CCGCGG	1 cut(s)	1530
SalI	GTCGAC	3 cut(s)	205, 977, 1160
SapI	GCTCTTC	1 cut(s)	1572
Sau3AI	GATC	8 cut(s)	353, 645, 1038, 1142, 1494, 1538, 1548, 1742
Sau96I	GGNCC	8 cut(s)	21, 1519, 1524, 1626, 1659, 1710, 1711, 1987
SchI	GAGTC	3 cut(s)	202, 734, 2118
SduI	GDGCHC	7 cut(s)	494, 681, 1692, 1714, 1888, 1907, 2013
SfaNI	GCATC	2 cut(s)	419, 778
SfcI	CTRYAG	2 cut(s)	331, 1666
SfoI	GGCGCC	3 cut(s)	703, 954, 1858
Sfr274I	CTCGAG	2 cut(s)	1806, 1881
Sfr303I	CCGCGG	1 cut(s)	1530
SfuI	TTCGAA	1 cut(s)	2130
SgrBI	CCGCGG	1 cut(s)	1530
SgsI	GGCGCGCC	1 cut(s)	2056
SinI	GGWCC	2 cut(s)	1626, 1659
SlaI	CTCGAG	2 cut(s)	1806, 1881
SmiMI	CAYNNNNRTG	1 cut(s)	1980
SmlI	CTYRAG	3 cut(s)	1208, 1806, 1881
SmoI	CTYRAG	3 cut(s)	1208, 1806, 1881
Sse9I	AATT	3 cut(s)	143, 265, 1615
SspDI	GGCGCC	3 cut(s)	701, 952, 1856
SspMI	CTAG	1 cut(s)	1260
SstI	GAGCTC	3 cut(s)	1692, 1888, 1907
StyI	CCWWGG	1 cut(s)	1221
TaaI	ACNGT	4 cut(s)	973, 1375, 1659, 1964
TaiI	ACGT	9 cut(s)	35, 306, 642, 938, 984, 1130, 1452, 1800, 2016
TasI	AATT	3 cut(s)	143, 265, 1615
TfiI	GAWTC	5 cut(s)	435, 811, 845, 1009, 1820
TseFI	GTSAC	3 cut(s)	4, 967, 1798
Tsp45I	GTSAC	3 cut(s)	4, 967, 1798
TspDTI	ATGAA	1 cut(s)	2117
TspGWI	ACGGA	2 cut(s)	1132, 1642
Tth111I	GACNNNGTC	1 cut(s)	937
Van91I	CCANNNNNTGG	1 cut(s)	1984
VpaK11BI	GGWCC	2 cut(s)	1626, 1659
XagI	CCTNNNNNAGG	1 cut(s)	1099
XapI	RAATTY	1 cut(s)	143
XhoI	CTCGAG	2 cut(s)	1806, 1881
XmiI	GTMKAC	4 cut(s)	206, 663, 978, 1161
XspI	CTAG	1 cut(s)	1260
ZraI	GACGTC	5 cut(s)	640, 936, 982, 1128, 1450

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.