MD15G1351000.v1.1
ERF Family

superfamily. Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006838
Physical Location & Seq
Reverse (-)
41713455 .. 41723087
9633 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1351000.v1.1.491

Sequence Viewer

Length: 3582 bp
ATGGCCGATTCAGCTCAGAACGAGTCGCCGACGACGCCTAAACGGCAAGTCGTTCGCCGGAAACTGGTCCAGACCACATTGTTCCCTCTCAAACCCCAGCAACAGCTCGAGGTAAACGGCGATCTGAAGGCCGCGAGAGGTAATGCTGGTGACGACGAATACGGCGACGACGAGGACTTCTGTGGGAGTCAAAGTAAAACGAAGAGGAAGTCAAAGGGAAAGAAGACGCCTCCCGTTAAAGCCCCGAAGAAGGACGGTAAGCGATCCACGAATGGTACACCGAAGAAGAAGATTAATGGAACTGAAGTGGAGAGTGAAGATGCGCCTCGAGTAGTCACCGATTTGAGGCTAGAGGCTAGATTGAAAGCTGAGGAAACATCACGGATATTTTCAGGAATGTTTTCTGGAAAGCAAATACATCCATTTTTTTCAATTTGTAAAGCTGGAAAAAGAAAGCGAGAGGCAACTGAAGTTGATGGCGATTTGAGCTACGTTGGGAGGAAGGATAAAGAGATCACTAGTGGTCCGATTCATGTGTTTGAAAGGACTCAGGATGATGCTGTGTTCCTTGATTGGAGAAACTGGACATTTTGTGAGGACACGTTTATGAAAAGTGGTCCAGACATGGAATGCACGTCCTCATCAATTTTTGAAGGCTCTGTAGAGTGCTTAAATTTTGAGAAGCTTCCCATTGTTTTCCAACCCTGCAAATTATCGACTTTTCAGAATGTGGTATCCTTAGATCAGCATTGTATTCAACAAGAATTCGCTCATGATATATCACCAACAGTCCCTGGCTTCTTAGTCGATGAGCAATTGATGCACTATCAGCAGTCAAAGGAAGTAGAGGATAATGAGATGCTTAAGGTTGATTTGCTTTCTCAGCATACCACTTATATAAAGAAGTCAGATATTGAGCAGCAGAAAATTCTCGAGGAAAGGTTGATGTCAAACTATTCCAGTTGTGGTAATCAGCCCACAAATAGCTTATGGACATATAAGTACCGGCCAATGAAGGCTATTGATGTATGTGGTAACCATGAATCTGTGAATTTTTTGAGTGAGTGGCTTCGTCTTTGGTATGAAAGAGATTTTCGAGCCAACAGAGATCTCACTAGGTGTTCTCAAAGTGGCTCTGATTTAGAAAGTGAAGATGAAGAGGCAAGCATGAAGAAGAATAATGTTCTCTTAGTTACAGGGCCAACTGGGAGTGGAAAATCTGCAGCTATTTATGCTTGTGCCCAAGAGCAAGGTTTTAAGGTTTTGGAGCTCAGTGCATCAGAATGTCGGAATGGGGCTCTTGTAAAACAGAGGTTTGGAGAGGCTTTGAAATCTCGCAATTTTAGAAGATCAGTGGCAAATCCTGAGGGTTCACAGAACAAGTCTATAGTGAAATCCCTGTTTGTCGATGCAAATGGTATGACAGACCTTGAGATGGCTGATGATGTTATTGAATTGATAGCCGTATCAGATGAAGATTCTCATGAGGCAACTGAAACCTCTGTAAAATCCGTCTCTAAGGAGGATTATAGCGAAGTTAAACATCTTATACTTTTTGACGATGTGGACATCATTTTTCTTGAAGATCGTGGTTTCATTGCAGCAATTCAGCAGATAGCTAAAACAGCCAAGGGGCCCATAATACTGACCAGCAATAGTGACAATCCTGTCCTGCCAGACAGTTTTGATAGATTACAAGTGCGTTTCACGTTGCCATCACAAAAGGAGCTGCATTCTCTTGCATATATGGTTTCTGCTTCGGAGAGAGCCAACATACAACCTTTGCTTCTTGAGCGACTTATTGAGTGTTGTCGGGGAGATATTCGGAAGATCTTTATGCATCTTCAGTTCTGGTGTCAGGGTGGAAGTTTTAGAAAAGATACCAAAATGCGAGAGTGGTATGGTTCCCTGCTATTTGATGTTGAGGCTGGCCATCTGATGCTGCCAAATATGTTACCCTGGGATTTACCTTCTCAGTTATCTGATGTAGTAGAGAAAGAGATAACAAAGGCATTATACATGATGGAAGAAAGTCCAAGATCAATGGACGTAATTGAAGAAAATCTTCACGAGATAGAAGTGCAATACAGTATGGACATGCCTTGTAATGGAATGGAAAGTTTAGAGGCCAAGAAGGTAGAAATGCTGAGCAGGAATGGCTCTGTTCATGACTGCTATCACTATACAGCTCAAACTGATACTGCTTCTGAGTTTTCCAACGATTTAGGTACCCCATTTTCCTCGTGTCGGCGTCATGTTCGGAATATGCAAGCTGTTGTAATGTCTGATTCTGAAGATGAGTTTATGACCAATGGATATCGTATAGTTACAGATAACGCTAACGATGAAGTCCTTGGTATTAACCCATTATCTGAGGAGCTACTTATTTTTGGAGCGGCTAATATAGATGGACCTTGTGAATGTTCAGAACTTGCAGACGAGGTGCACATAAGCGAGACTTGCAACTCAGTTGATATATCATGTGTACCGGAATCGTCTTTTGTTCCTGAAACTGAAATTGATAATATGCCAGAATTATCTTCTCAAACAGTGTCTTCTGACCATTTTGCCAACGCCATTAAAAGAGTATCTTTAGACGATGAATTACATGGTGGAGCCAGCAATCTTAATAAACTAACCTTCGTACAAGGTAACTCTGATAAGTGGGGAAGTGGCTGTGCTACAATTGCAGAGTATTCTCACAAGGAGTTTGAGAATCAAAAGGAACATGCGGAGACTGTTGCAAGGGCTTATCAGTTGATGGATGAGTGTAGCCACATGGATTTTAATGAGGGCTCGCGGTTTATACAGGGCCAAAAGTCCTCAGCGGTGACAGATTTAGTACAAGATTCATGGGACAAACTTCGTGGCTCCCGCATTGATCTTAGACAGTATATTGCATTCGAACAACCCAATGCCTCTCAGATTGTCATGCTTGCTGATCAAATGAGCAATTTGATCTCAGAAACTGACATGTTGTTTTCTAAATGCCAATCATTGACAAATGATTCTTTTGAACTATCAATGATCCCCTTGGAGGAGTCAGATGCATACAGCTGGTGTGATGAGCGATTATTGTTGGCGTCTACAATTGCACAGCATGGGTTTTGCTTCTATGCAAAACGCATTTCTTCTGTAGGATCAAAAGAAGGTAGTGTAGGGAGGGTGGATTTGGCTGGGGAAATGCTGGCTAACACAGCCAGTACGATGGCATTCAGTAAGTTAATTGGGAAGGGTATGAGAGCAAGTAAGGATTCATCTGCCGAAAGAAATTCAGAGACGTTCCTGCCAAATGCGACAAGTGAAATCCAATCACGTGTATTTGATGTAATTCAGTCAATTGTACCTTCAAGAACGTACTCAAATTTGAGAGGTGGTGCACACCATGAGTATCTTTCTTCTCTGCGCCACATTTCAAGATCAGAAGCTTCTCGGTTATCAGAAGGAGCTGAGAAGACCACCAGAAGTAGGAGGAGGAGGGTTGCTCCGCACTACTTAAGTAGTGGTGCACTGATGTTATCTCCTGAACATATATCGTTGCTGGATCAGTATGGCTCATACGGGAAAACCTCTTCACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0001775 GO:0002200 GO:0002204 GO:0002208 GO:0002250 GO:0002252 GO:0002263 GO:0002285 GO:0002312 GO:0002366 GO:0002376 GO:0002377 GO:0002381 GO:0002440 GO:0002443 GO:0002449 GO:0002460 GO:0002520 GO:0002562 GO:0002637 GO:0002639 GO:0002682 GO:0002684 GO:0002694 GO:0002696 GO:0002697 GO:0002699 GO:0002700 GO:0002702 GO:0002703 GO:0002705 GO:0002706 GO:0002708 GO:0002712 GO:0002714 GO:0002819 GO:0002821 GO:0002822 GO:0002824 GO:0002889 GO:0002891 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006310 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0006955 GO:0006974 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0008283 GO:0008284 GO:0008630 GO:0009058 GO:0009059 GO:0009893 GO:0009966 GO:0009968 GO:0009987 GO:0010564 GO:0010604 GO:0010646 GO:0010648 GO:0010941 GO:0012501 GO:0016064 GO:0016444 GO:0016445 GO:0016447 GO:0019219 GO:0019222 GO:0019724 GO:0022402 GO:0023051 GO:0023052 GO:0023057 GO:0030888 GO:0030890 GO:0031323 GO:0031325 GO:0032501 GO:0032502 GO:0032943 GO:0032944 GO:0032946 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0035556 GO:0042100 GO:0042113 GO:0042127 GO:0042770 GO:0042771 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0045190 GO:0045191 GO:0045321 GO:0045830 GO:0045911 GO:0045935 GO:0046483 GO:0046649 GO:0046651 GO:0048302 GO:0048304 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048731 GO:0048856 GO:0050670 GO:0050671 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0050867 GO:0050871 GO:0050896 GO:0051052 GO:0051054 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051249 GO:0051251 GO:0051716 GO:0051726 GO:0060255 GO:0060548 GO:0065007 GO:0070661 GO:0070663 GO:0070665 GO:0071704 GO:0072331 GO:0072332 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0097190 GO:0097193 GO:1901360 GO:1901576 GO:1901796 GO:1901797 GO:1901987 GO:1901990 GO:1902165 GO:1902166 GO:1902229 GO:1902230 GO:1902253 GO:1902254 GO:1902531 GO:1902532 GO:2000026 GO:2001020 GO:2001021 GO:2001233 GO:2001234 GO:2001242 GO:2001243
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA Rad17

Protein Analysis

1194

Amino Acids

133.87

Weight (kDa)

5.15

Isoelectric Point (pI)

50.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1667
Acc65I GGTACC 1 cut(s) 2228
AccB1I GGYRCC 1 cut(s) 2228
AccBSI CCGCTC 1 cut(s) 2396
AccI GTMKAC 1 cut(s) 3086
AccII CGCG 2 cut(s) 134, 2800
AciI CCGC 7 cut(s) 132, 2396, 2732, 2800, 2828, 2875, 3488
AclWI GGATC 4 cut(s) 258, 3022, 3148, 3552
AcoI YGGCCR 3 cut(s) 3, 1007, 1930
AcsI RAATTY 6 cut(s) 673, 764, 927, 1051, 3271, 3364
AcuI CTGAAG 5 cut(s) 146, 324, 489, 1829, 2313
AcvI CACGTG 1 cut(s) 3317
AcyI GRCGYC 4 cut(s) 35, 227, 2251, 3083
AdeI CACNNNGTG 1 cut(s) 1119
AfaI GTAC 9 cut(s) 277, 1004, 2230, 2487, 2646, 2844, 3205, 3345, 3359
AfiI CCNNNNNNNGG 8 cut(s) 64, 250, 345, 1435, 2108, 2247, 3037, 3468
AflII CTTAAG 2 cut(s) 863, 3496
AflIII ACRYGT 3 cut(s) 600, 2973, 3316
AhlI ACTAGT 1 cut(s) 518
AjiI CACGTC 1 cut(s) 636
AjnI CCWGG 2 cut(s) 793, 1958
Alw21I GWGCWC 4 cut(s) 1272, 2448, 3382, 3511
Alw26I GTCTC 4 cut(s) 1519, 2450, 2729, 3272
Alw44I GTGCAC 3 cut(s) 2444, 3378, 3507
AlwI GGATC 4 cut(s) 258, 3022, 3148, 3552
AlwNI CAGNNNCTG 2 cut(s) 794, 2969
Ama87I CYCGRG 3 cut(s) 107, 327, 932
AoxI GGCC 8 cut(s) 3, 129, 1007, 1199, 1634, 1930, 2127, 2812
ApaI GGGCCC 1 cut(s) 1638
ApaLI GTGCAC 3 cut(s) 2444, 3378, 3507
ApeKI GCWGC 5 cut(s) 919, 1223, 1601, 1729, 1942
ApoI RAATTY 6 cut(s) 673, 764, 927, 1051, 3271, 3364
ArsI GACNNNNNNTTYG 2 cut(s) 194, 226
AseI ATTAAT 1 cut(s) 294
Asp700I GAANNNNTTC 2 cut(s) 400, 2064
Asp718I GGTACC 1 cut(s) 2228
AspLEI GCGC 2 cut(s) 325, 3408
AspS9I GGNCC 8 cut(s) 67, 524, 617, 1199, 1634, 1635, 2411, 2812
AsuHPI GGTGA 4 cut(s) 161, 328, 774, 2842
AsuII TTCGAA 1 cut(s) 2904
AvaI CYCGRG 3 cut(s) 107, 327, 932
AvaII GGWCC 4 cut(s) 67, 524, 617, 2411
AxyI CCTNAGG 1 cut(s) 1365
BaeGI GKGCMC 5 cut(s) 1243, 1638, 2448, 3382, 3511
BalI TGGCCA 1 cut(s) 1932
BanI GGYRCC 1 cut(s) 2228
BanII GRGCYC 4 cut(s) 1272, 1300, 1638, 2798
BauI CACGAG 2 cut(s) 2069, 2242
BbrPI CACGTG 1 cut(s) 3317
BbsI GAAGAC 3 cut(s) 230, 2547, 3461
Bbv12I GWGCWC 4 cut(s) 1272, 2448, 3382, 3511
BbvCI CCTCAGC 2 cut(s) 369, 2824
BbvI GCAGC 5 cut(s) 931, 1235, 1613, 1716, 1929
BccI CCATC 8 cut(s) 470, 1429, 1723, 1941, 2017, 2402, 2755, 3202
BceAI ACGGC 4 cut(s) 59, 133, 178, 1448
BcgI CGANNNNNNTGC 2 cut(s) 35, 69
BciT130I CCWGG 2 cut(s) 795, 1960
BciVI GTATCC 1 cut(s) 745
BclI TGATCA 1 cut(s) 2941
BcoDI GTCTC 4 cut(s) 1519, 2450, 2729, 3272
BcuI ACTAGT 1 cut(s) 518
BfaI CTAG 4 cut(s) 350, 357, 519, 1116
BfmI CTRYAG 4 cut(s) 660, 1221, 1386, 3135
BfrI CTTAAG 2 cut(s) 863, 3496
BfuI GTATCC 1 cut(s) 745
BglI GCCNNNNNGGC 1 cut(s) 43
BglII AGATCT 2 cut(s) 1108, 1830
BisI GCNGC 7 cut(s) 132, 920, 1224, 1602, 1730, 1943, 2397
BlpI GCTNAGC 1 cut(s) 2147
BlsI GCNGC 7 cut(s) 133, 921, 1225, 1603, 1731, 1944, 2398
Bme1390I CCNGG 2 cut(s) 795, 1960
Bme18I GGWCC 4 cut(s) 67, 524, 617, 2411
BmeT110I CYCGRG 3 cut(s) 107, 327, 932
BmgBI CACGTC 1 cut(s) 636
BmgT120I GGNCC 8 cut(s) 67, 524, 617, 1199, 1634, 1635, 2411, 2812
BmiI GGNNCC 6 cut(s) 1635, 1636, 1906, 2230, 2617, 2872
BmrFI CCNGG 2 cut(s) 795, 1960
BmrI ACTGGG 1 cut(s) 1215
BmsI GCATC 9 cut(s) 310, 547, 810, 849, 1286, 1399, 1849, 1929, 3037
BmuI ACTGGG 1 cut(s) 1215
BpiI GAAGAC 3 cut(s) 230, 2547, 3461
BplI GAGNNNNNCTC 2 cut(s) 3469, 3501
Bpu10I CCTNAGC 2 cut(s) 369, 2824
Bpu1102I GCTNAGC 1 cut(s) 2147
Bpu14I TTCGAA 1 cut(s) 2904
BpuEI CTTGAG 2 cut(s) 1451, 1811
BsaAI YACGTR 1 cut(s) 3317
BsaHI GRCGYC 4 cut(s) 35, 227, 2251, 3083
BsaJI CCNNGG 6 cut(s) 793, 1629, 1958, 1959, 2353, 3033
BsaWI WCCGGW 1 cut(s) 2488
BsaXI ACNNNNNCTCC 2 cut(s) 178, 208
Bsc4I CCNNNNNNNGG 8 cut(s) 64, 250, 345, 1435, 2108, 2247, 3037, 3468
Bse118I RCCGGY 1 cut(s) 1005
Bse1I ACTGG 5 cut(s) 69, 587, 960, 1210, 3201
Bse21I CCTNAGG 1 cut(s) 1365
Bse3DI GCAATG 1 cut(s) 1596
BseBI CCWGG 2 cut(s) 795, 1960
BseDI CCNNGG 6 cut(s) 793, 1629, 1958, 1959, 2353, 3033
BseGI GGATG 3 cut(s) 418, 559, 2770
BseLI CCNNNNNNNGG 8 cut(s) 64, 250, 345, 1435, 2108, 2247, 3037, 3468
BseMI GCAATG 1 cut(s) 1596
BseNI ACTGG 5 cut(s) 69, 587, 960, 1210, 3201
BseRI GAGGAG 4 cut(s) 2390, 3053, 3487, 3490
BseSI GKGCMC 5 cut(s) 1243, 1638, 2448, 3382, 3511
BseXI GCAGC 5 cut(s) 931, 1235, 1613, 1716, 1929
BseYI CCCAGC 2 cut(s) 96, 3176
Bsh1236I CGCG 2 cut(s) 134, 2800
BshFI GGCC 8 cut(s) 5, 131, 1009, 1201, 1636, 1932, 2129, 2814
BshNI GGYRCC 1 cut(s) 2228
BsiHKAI GWGCWC 4 cut(s) 1272, 2448, 3382, 3511
BsiHKCI CYCGRG 3 cut(s) 107, 327, 932
BsiSI CCGG 3 cut(s) 58, 1006, 2489
BslFI GGGAC 2 cut(s) 776, 2870
BslI CCNNNNNNNGG 8 cut(s) 64, 250, 345, 1435, 2108, 2247, 3037, 3468
BsmAI GTCTC 4 cut(s) 1519, 2450, 2729, 3272
BsmBI CGTCTC 2 cut(s) 1519, 3272
BsmFI GGGAC 2 cut(s) 776, 2870
BsmI GAATGC 4 cut(s) 635, 1732, 2900, 3212
BsnI GGCC 8 cut(s) 5, 131, 1009, 1201, 1636, 1932, 2129, 2814
BsoBI CYCGRG 3 cut(s) 107, 327, 932
Bsp119I TTCGAA 1 cut(s) 2904
Bsp120I GGGCCC 1 cut(s) 1634
Bsp1286I GDGCHC 8 cut(s) 1243, 1272, 1300, 1638, 2448, 2798, 3382, 3511
Bsp1720I GCTNAGC 1 cut(s) 2147
BspACI CCGC 7 cut(s) 132, 2396, 2732, 2800, 2828, 2875, 3488
BspANI GGCC 8 cut(s) 5, 131, 1009, 1201, 1636, 1932, 2129, 2814
BspFNI CGCG 2 cut(s) 134, 2800
BspHI TCATGA 3 cut(s) 772, 1483, 2167
BspLI GGNNCC 6 cut(s) 1635, 1636, 1906, 2230, 2617, 2872
BspMAI CTGCAG 1 cut(s) 1225
BspPI GGATC 4 cut(s) 258, 3022, 3148, 3552
BspT104I TTCGAA 1 cut(s) 2904
BspT107I GGYRCC 1 cut(s) 2228
BspTI CTTAAG 2 cut(s) 863, 3496
BsrBI CCGCTC 1 cut(s) 2396
BsrDI GCAATG 1 cut(s) 1596
BsrFI RCCGGY 1 cut(s) 1005
BsrI ACTGG 5 cut(s) 69, 587, 960, 1210, 3201
BssAI RCCGGY 1 cut(s) 1005
BssECI CCNNGG 6 cut(s) 793, 1629, 1958, 1959, 2353, 3033
BssNI GRCGYC 4 cut(s) 35, 227, 2251, 3083
BssSI CACGAG 2 cut(s) 2069, 2242
BssT1I CCWWGG 3 cut(s) 1629, 2353, 3033
Bst2BI CACGAG 2 cut(s) 2069, 2242
Bst2UI CCWGG 2 cut(s) 795, 1960
Bst4CI ACNGT 7 cut(s) 257, 790, 1682, 2090, 2551, 2740, 2892
Bst6I CTCTTC 3 cut(s) 197, 1152, 3577
BstACI GRCGYC 4 cut(s) 35, 227, 2251, 3083
BstAFI CTTAAG 2 cut(s) 863, 3496
BstAPI GCANNNNNTGC 1 cut(s) 820
BstBAI YACGTR 1 cut(s) 3317
BstBI TTCGAA 1 cut(s) 2904
BstC8I GCNNGC 7 cut(s) 1165, 1930, 2271, 2620, 2798, 2937, 3189
BstEII GGTNACC 1 cut(s) 1034
BstF5I GGATG 3 cut(s) 418, 559, 2770
BstFNI CGCG 2 cut(s) 134, 2800
BstHHI GCGC 2 cut(s) 325, 3408
BstMAI GTCTC 4 cut(s) 1519, 2450, 2729, 3272
BstNI CCWGG 2 cut(s) 795, 1960
BstNSI RCATGY 3 cut(s) 2101, 2732, 2977
BstPI GGTNACC 1 cut(s) 1034
BstSCI CCNGG 2 cut(s) 793, 1958
BstSFI CTRYAG 4 cut(s) 660, 1221, 1386, 3135
BstSLI GKGCMC 5 cut(s) 1243, 1638, 2448, 3382, 3511
BstUI CGCG 2 cut(s) 134, 2800
BstV1I GCAGC 5 cut(s) 931, 1235, 1613, 1716, 1929
BstV2I GAAGAC 3 cut(s) 230, 2547, 3461
BstX2I RGATCY 2 cut(s) 1108, 1830
BstXI CCANNNNNNTGG 1 cut(s) 3208
BstYI RGATCY 2 cut(s) 1108, 1830
Bsu36I CCTNAGG 1 cut(s) 1365
BsuI GTATCC 1 cut(s) 745
BsuRI GGCC 8 cut(s) 5, 131, 1009, 1201, 1636, 1932, 2129, 2814
BtrI CACGTC 1 cut(s) 636
BtsCI GGATG 3 cut(s) 418, 559, 2770
BtsIMutI CAGTG 5 cut(s) 1279, 1359, 2556, 3509, 3577
Cac8I GCNNGC 7 cut(s) 1165, 1930, 2271, 2620, 2798, 2937, 3189
CaiI CAGNNNCTG 2 cut(s) 794, 2969
CciI TCATGA 3 cut(s) 772, 1483, 2167
CfoI GCGC 2 cut(s) 325, 3408
Cfr10I RCCGGY 1 cut(s) 1005
Cfr13I GGNCC 8 cut(s) 67, 524, 617, 1199, 1634, 1635, 2411, 2812
CseI GACGC 4 cut(s) 43, 235, 2240, 3072
Csp6I GTAC 9 cut(s) 276, 1003, 2229, 2486, 2645, 2843, 3204, 3344, 3358
CspCI CAANNNNNGTGG 2 cut(s) 2848, 2883
CviQI GTAC 9 cut(s) 276, 1003, 2229, 2486, 2645, 2843, 3204, 3344, 3358
DraIII CACNNNGTG 1 cut(s) 1119
DrdI GACNNNNNNGTC 1 cut(s) 1667
DseDI GACNNNNNNGTC 1 cut(s) 1667
EaeI YGGCCR 3 cut(s) 3, 1007, 1930
Eam1104I CTCTTC 3 cut(s) 197, 1152, 3577
EarI CTCTTC 3 cut(s) 197, 1152, 3577
Ecl136II GAGCTC 1 cut(s) 1270
Eco130I CCWWGG 3 cut(s) 1629, 2353, 3033
Eco24I GRGCYC 4 cut(s) 1272, 1300, 1638, 2798
Eco32I GATATC 1 cut(s) 2318
Eco47I GGWCC 4 cut(s) 67, 524, 617, 2411
Eco53kI GAGCTC 1 cut(s) 1270
Eco57I CTGAAG 5 cut(s) 146, 324, 489, 1829, 2313
Eco72I CACGTG 1 cut(s) 3317
Eco81I CCTNAGG 1 cut(s) 1365
Eco88I CYCGRG 3 cut(s) 107, 327, 932
Eco91I GGTNACC 1 cut(s) 1034
EcoICRI GAGCTC 1 cut(s) 1270
EcoO109I RGGNCCY 1 cut(s) 1634
EcoO65I GGTNACC 1 cut(s) 1034
EcoRI GAATTC 1 cut(s) 764
EcoRII CCWGG 2 cut(s) 793, 1958
EcoRV GATATC 1 cut(s) 2318
EcoT14I CCWWGG 3 cut(s) 1629, 2353, 3033
EcoT22I ATGCAT 2 cut(s) 1842, 3052
EcoT38I GRGCYC 4 cut(s) 1272, 1300, 1638, 2798
ErhI CCWWGG 3 cut(s) 1629, 2353, 3033
Esp3I CGTCTC 2 cut(s) 1519, 3272
FalI AAGNNNNNCTT 6 cut(s) 2049, 2081, 2443, 2475, 2575, 2607
FaqI GGGAC 2 cut(s) 776, 2870
FauI CCCGC 1 cut(s) 2882
FbaI TGATCA 1 cut(s) 2941
FblI GTMKAC 1 cut(s) 3086
Fnu4HI GCNGC 7 cut(s) 132, 920, 1224, 1602, 1730, 1943, 2397
FokI GGATG 3 cut(s) 405, 566, 2777
FriOI GRGCYC 4 cut(s) 1272, 1300, 1638, 2798
Fsp4HI GCNGC 7 cut(s) 132, 920, 1224, 1602, 1730, 1943, 2397
FspBI CTAG 4 cut(s) 350, 357, 519, 1116
GlaI GCGC 2 cut(s) 324, 3407
GluI GCNGC 7 cut(s) 132, 920, 1224, 1602, 1730, 1943, 2397
GsaI CCCAGC 2 cut(s) 100, 3180
HaeIII GGCC 8 cut(s) 5, 131, 1009, 1201, 1636, 1932, 2129, 2814
HapII CCGG 3 cut(s) 58, 1006, 2489
HgaI GACGC 4 cut(s) 43, 235, 2240, 3072
HhaI GCGC 2 cut(s) 325, 3408
Hin1I GRCGYC 4 cut(s) 35, 227, 2251, 3083
Hin6I GCGC 2 cut(s) 323, 3406
HinP1I GCGC 2 cut(s) 323, 3406
HindIII AAGCTT 2 cut(s) 683, 3426
HpaII CCGG 3 cut(s) 58, 1006, 2489
HphI GGTGA 4 cut(s) 161, 328, 774, 2842
Hpy166II GTNNAC 9 cut(s) 115, 278, 1373, 1567, 2446, 2486, 3087, 3380, 3509
Hpy8I GTNNAC 9 cut(s) 115, 278, 1373, 1567, 2446, 2486, 3087, 3380, 3509
Hpy99I CGWCG 5 cut(s) 34, 37, 158, 170, 173
HpyCH4III ACNGT 7 cut(s) 257, 790, 1682, 2090, 2551, 2740, 2892
HpyCH4IV ACGT 8 cut(s) 492, 602, 635, 1709, 2049, 3281, 3316, 3356
HpySE526I ACGT 8 cut(s) 492, 602, 635, 1709, 2049, 3281, 3316, 3356
Hsp92I GRCGYC 4 cut(s) 35, 227, 2251, 3083
HspAI GCGC 2 cut(s) 323, 3406
KpnI GGTACC 1 cut(s) 2232
Ksp22I TGATCA 1 cut(s) 2941
LmnI GCTCC 8 cut(s) 1267, 1726, 2377, 2393, 2615, 2876, 3446, 3490
Lsp1109I GCAGC 5 cut(s) 931, 1235, 1613, 1716, 1929
LweI GCATC 9 cut(s) 310, 547, 810, 849, 1286, 1399, 1849, 1929, 3037
MaeI CTAG 4 cut(s) 350, 357, 519, 1116
MaeII ACGT 8 cut(s) 492, 602, 635, 1709, 2049, 3281, 3316, 3356
MaeIII GTNAC 9 cut(s) 149, 334, 1034, 1192, 1658, 1953, 2326, 2651, 2830
MbiI CCGCTC 1 cut(s) 2396
MfeI CAATTG 4 cut(s) 815, 2685, 3090, 3339
MflI RGATCY 2 cut(s) 1108, 1830
MhlI GDGCHC 8 cut(s) 1243, 1272, 1300, 1638, 2448, 2798, 3382, 3511
MlsI TGGCCA 1 cut(s) 1932
MluNI TGGCCA 1 cut(s) 1932
MlyI GAGTC 4 cut(s) 32, 196, 541, 3050
MmeI TCCRAC 3 cut(s) 724, 1268, 2241
Mox20I TGGCCA 1 cut(s) 1932
Mph1103I ATGCAT 2 cut(s) 1842, 3052
MroXI GAANNNNTTC 2 cut(s) 400, 2064
MscI TGGCCA 1 cut(s) 1932
MslI CAYNNNNRTG 2 cut(s) 605, 1282
Msp20I TGGCCA 1 cut(s) 1932
MspA1I CMGCKG 2 cut(s) 2828, 3057
MspCI CTTAAG 2 cut(s) 863, 3496
MspI CCGG 3 cut(s) 58, 1006, 2489
MspR9I CCNGG 2 cut(s) 795, 1960
MunI CAATTG 4 cut(s) 815, 2685, 3090, 3339
Mva1269I GAATGC 4 cut(s) 635, 1732, 2900, 3212
MvaI CCWGG 2 cut(s) 795, 1960
MvnI CGCG 2 cut(s) 134, 2800
NlaIV GGNNCC 6 cut(s) 1635, 1636, 1906, 2230, 2617, 2872
NmuCI GTSAC 4 cut(s) 149, 334, 1658, 2830
NsiI ATGCAT 2 cut(s) 1842, 3052
NspI RCATGY 3 cut(s) 2101, 2732, 2977
NspV TTCGAA 1 cut(s) 2904
PaeR7I CTCGAG 3 cut(s) 107, 327, 932
PagI TCATGA 3 cut(s) 772, 1483, 2167
PasI CCCWGGG 1 cut(s) 1959
PciI ACATGT 1 cut(s) 2973
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 168
PctI GAATGC 4 cut(s) 635, 1732, 2900, 3212
PdmI GAANNNNTTC 2 cut(s) 400, 2064
PkrI GCNGC 7 cut(s) 133, 921, 1225, 1603, 1731, 1944, 2398
PleI GAGTC 4 cut(s) 31, 195, 541, 3049
PmaCI CACGTG 1 cut(s) 3317
PmlI CACGTG 1 cut(s) 3317
PpsI GAGTC 4 cut(s) 31, 195, 541, 3049
Ppu21I YACGTR 1 cut(s) 3317
PscI ACATGT 1 cut(s) 2973
PshBI ATTAAT 1 cut(s) 294
Psp124BI GAGCTC 1 cut(s) 1272
Psp6I CCWGG 2 cut(s) 793, 1958
PspCI CACGTG 1 cut(s) 3317
PspEI GGTNACC 1 cut(s) 1034
PspFI CCCAGC 2 cut(s) 96, 3176
PspGI CCWGG 2 cut(s) 793, 1958
PspN4I GGNNCC 6 cut(s) 1635, 1636, 1906, 2230, 2617, 2872
PspOMI GGGCCC 1 cut(s) 1634
PspPI GGNCC 8 cut(s) 67, 524, 617, 1199, 1634, 1635, 2411, 2812
PspXI VCTCGAGB 2 cut(s) 107, 327
PstI CTGCAG 1 cut(s) 1225
PstNI CAGNNNCTG 2 cut(s) 794, 2969
PsuI RGATCY 2 cut(s) 1108, 1830
PvuII CAGCTG 1 cut(s) 3057
RsaI GTAC 9 cut(s) 277, 1004, 2230, 2487, 2646, 2844, 3205, 3345, 3359
RsaNI GTAC 9 cut(s) 276, 1003, 2229, 2486, 2645, 2843, 3204, 3344, 3358
RseI CAYNNNNRTG 2 cut(s) 605, 1282
SacI GAGCTC 1 cut(s) 1272
SatI GCNGC 7 cut(s) 132, 920, 1224, 1602, 1730, 1943, 2397
Sau96I GGNCC 8 cut(s) 67, 524, 617, 1199, 1634, 1635, 2411, 2812
SchI GAGTC 4 cut(s) 32, 196, 541, 3050
ScrFI CCNGG 2 cut(s) 795, 1960
SduI GDGCHC 8 cut(s) 1243, 1272, 1300, 1638, 2448, 2798, 3382, 3511
SfaNI GCATC 9 cut(s) 310, 547, 810, 849, 1286, 1399, 1849, 1929, 3037
SfcI CTRYAG 4 cut(s) 660, 1221, 1386, 3135
Sfr274I CTCGAG 3 cut(s) 107, 327, 932
SfuI TTCGAA 1 cut(s) 2904
SinI GGWCC 4 cut(s) 67, 524, 617, 2411
SlaI CTCGAG 3 cut(s) 107, 327, 932
SmiMI CAYNNNNRTG 2 cut(s) 605, 1282
SmlI CTYRAG 7 cut(s) 107, 327, 863, 932, 1430, 1790, 3496
SmoI CTYRAG 7 cut(s) 107, 327, 863, 932, 1430, 1790, 3496
SpeI ACTAGT 1 cut(s) 518
SsiI CCGC 7 cut(s) 132, 2396, 2732, 2800, 2828, 2875, 3488
SspMI CTAG 4 cut(s) 350, 357, 519, 1116
SstI GAGCTC 1 cut(s) 1272
StyD4I CCNGG 2 cut(s) 793, 1958
StyI CCWWGG 3 cut(s) 1629, 2353, 3033
TaaI ACNGT 7 cut(s) 257, 790, 1682, 2090, 2551, 2740, 2892
TaiI ACGT 8 cut(s) 495, 605, 638, 1712, 2052, 3284, 3319, 3359
TaqI TCGA 8 cut(s) 108, 328, 716, 807, 933, 1096, 1407, 2904
TatI WGTACW 1 cut(s) 2842
TauI GCSGC 2 cut(s) 134, 2399
TscAI CASTG 4 cut(s) 1279, 1359, 2556, 3516
TseFI GTSAC 4 cut(s) 149, 334, 1658, 2830
TseI GCWGC 5 cut(s) 919, 1223, 1601, 1729, 1942
Tsp45I GTSAC 4 cut(s) 149, 334, 1658, 2830
TspGWI ACGGA 2 cut(s) 397, 1501
TspRI CASTG 4 cut(s) 1279, 1359, 2556, 3516
Vha464I CTTAAG 2 cut(s) 863, 3496
VneI GTGCAC 3 cut(s) 2444, 3378, 3507
VpaK11BI GGWCC 4 cut(s) 67, 524, 617, 2411
VspI ATTAAT 1 cut(s) 294
XapI RAATTY 6 cut(s) 673, 764, 927, 1051, 3271, 3364
XceI RCATGY 3 cut(s) 2101, 2732, 2977
XhoI CTCGAG 3 cut(s) 107, 327, 932
XmiI GTMKAC 1 cut(s) 3086
XmnI GAANNNNTTC 2 cut(s) 400, 2064
XspI CTAG 4 cut(s) 350, 357, 519, 1116
Zsp2I ATGCAT 2 cut(s) 1842, 3052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.