Rorug06G0253100
ERF Family

superfamily. Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006838
Physical Location & Seq
Forward (+)
39965600 .. 39969970
4371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0253100.1

Sequence Viewer

Length: 2700 bp
ATGGCGTCCTTGAACAACCAGCCATCCAAGAAGGCAATACGCACCCCCGGAGGCTCAGCTGGTAGCTCTCAGGGTAAGGCTAATTCAAGTGGCCAAACTGTGAAATTCGCTAGACGAACTTCGAGCGGAAGGTATGTCAGTCTCTCAAGAGAAGACCTTGACATGTCAGGGGAATTGTCAGGGGACTACATGAACTACACAGTTCATATTCCTCCCACCCCGGATAACCAACCCATGGACACTTCTGTGGCTGTCAAGGCGGAGGAGCAATATGTTTCTAACTCACTCTTCACCGGGGGATTCAACAGCGTGACACGTGCTCATTTGATGGACAAGGTAATTGATTCTGAGGTGACACATCCACAGATGGCCGGAGCCAAAGGTTCGGCGTGCATGATGCCTGCTTGTGACGGTAAGGTGATGAAGGACGAGAGGGGAGTCGATATAACTCCTTGTGATTGCAGGTTCAAAATATGCAGAGATTGCTATATGGATGCACAGAAGGATACAAATCTTTGTCCTGGTTGCAAGGAGCCATACCGAATGGGGGACTTAGATGATGATGGACCAGATTATGAGCGTGGAGGAGCATTGCAATTGCCTGGACCAGATGGAAATAGGGATAACATGTCAATGATGAAGAGGAACCAAACAGGTGAATTTGATCACAACAGGTGGTTGTTTGAGACAAGGGGTACCTATGGTGTTGGTAATGCATTTAATCCCCCAGAGGACGGTGGTTATGATGAAGGGTTCGGACCAGGATCAATGGATGCTACCGAAAAAGCTTTTAAGCCGCTAAGTAGGAAGATACCAATTGCAGCAGCCGTTATCAGCCCTTACAGGTTACTGATCTTTGTTCGATTCATAGTGTTGTGTTTCTTCTTGCATTGGAGAGTAGTGCATCCAAACAATGACGCAAGATGGTTGTGGATGATGTCAATTGTTTGTGAGCTGTGGTTTGCCTTCTCCTGGATTTTGGATCAAACTCCAAAGTTCTTCCCTATTAATCGTGCTACAGACCTAGAAGTCCTATATGAAAAGTTTGATATGCCATCTCCTTCCAATCCCACTGGTCGCTCTGACCTCCCCGGCATTGACATGTTTGTCTCTACTGCTGATCCTGACAAGGAGCCACCTCTTACCACTGCTAACACCATTCTTTCAATCTTGGCTGTTGATTACCCGGTGGAGAAGATTGCATGCTACATCTCCGATGACGGAGGTGCTCTTCTTACTTTTGAGGCAATGGCAGAGGCTGCTAGTTTTGCAGATTTGTGGGTGCCATTCTGCAAGAAACACACTATTGAACCAAGAAATCCTGATAGTTACTTCGCCTTAAAAGTTGACCCTACCAAGAACAAGAGCAGGACTGACTTTGTGAAGGATAGAAGGAAGATCAAAAGGGAGTATGATGAGTTCAAGGTAAGGATTAACGGGCTTCCTGACTCTATCAGGAGAAGATCTGATGCCTTCCATGCCAGGGAGGAAATGAAAATGTTAAAGCACATGAGGGAGACCGGTGCTGACCCTTTGGAGCAAGTCAAGGTCCCAAAGGCCACGTGGATGGCTGATGGCACACATTGGCCGGGTACTTGGTCCGTCCCTTCTAGTGACCATGCGAAAGGTGACCATTCAGGAATTCTACAGGTGATGCTCAAGCCTCCTAGCCCTGATCCACTAATGGGAGGGGCAGATGACAAGCTCTTAGATTTCACAGATGTTGATATACGACTCCCAATGTTTGTGTACATGTCAAGAGAGAAGCGGCCAGGCTATGATCACAACAAGAAAGCTGGTGCCATGAATGCCTTGGTACGAACTTCAGCCATCTTGTCCAATGGGCCATTCATCCTCAACCTCGACTGTGACCACTACATCTACAACTGCAAGGCCATCCGAGAAGGAATGTGCTTCATGATGGACCGAGGAGGAGAAGGTATCTGCTACATTCAGTTCCCTCAGAGATTTGAAGGCATTGATCCCTCGGATCGTTATGCCAACCATAACACTGTGTTTTTCGATGGCAATATGCGTGCTCTTGATGGTCTTCAAGGTCCAATGTACGTGGGAACTGGGACAATGTTTAGGCGGTTTGCCCTTTATGGTTTTGACCCTCCAAATCCTGACAAGGTCGCCGCACAGAAGGCGGCAACAGAAGCTACTGGTGACTCAGAGACACAAAAAGAGCCCTTGACGGCACCTCCGGCACAGACCACACAGCCCTTGACAGCTAGTGAACTCGACCCTGATCTTGATACCAATCTGCTGCCTAAGCGTTTTGGAAATTCTACCATGTTAGCTGAATCCATACCTGTTGCTGAGTATCAAGGACGCCCCCTAGCTGATCATCCTGCAGTCAAATTCGGACGCCCTCCTGGTATTCTTAGGGTACCACGTGATCCACTTGATGCTACAACTGTTGCTGAAGCTGTCTCTGCCATTTCTTGCTGGTACGAGGACAAGACTGAATGGGGAGACCGAGTGGGTTGGATTTATGGATCAGTCACAGAAGATGTTGTGACAGGCTACCGTATGCACAACCGTGGATGGCATTCAGTTTATTGTATCACCAAGCGTGATGCATTCCGGGGTTCAGCTCCGATTAATCTTACTGATCGACTCCACCAAGTGCTTAGGGCTGGCCCTGCTAGAGGTGAAGTGGTCTGGCATAGGATTGGAAGAGTGGTGGAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0001775 GO:0002200 GO:0002204 GO:0002208 GO:0002250 GO:0002252 GO:0002263 GO:0002285 GO:0002312 GO:0002366 GO:0002376 GO:0002377 GO:0002381 GO:0002440 GO:0002443 GO:0002449 GO:0002460 GO:0002520 GO:0002562 GO:0002637 GO:0002639 GO:0002682 GO:0002684 GO:0002694 GO:0002696 GO:0002697 GO:0002699 GO:0002700 GO:0002702 GO:0002703 GO:0002705 GO:0002706 GO:0002708 GO:0002712 GO:0002714 GO:0002819 GO:0002821 GO:0002822 GO:0002824 GO:0002889 GO:0002891 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006310 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0006955 GO:0006974 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0008283 GO:0008284 GO:0008630 GO:0009058 GO:0009059 GO:0009893 GO:0009966 GO:0009968 GO:0009987 GO:0010564 GO:0010604 GO:0010646 GO:0010648 GO:0010941 GO:0012501 GO:0016064 GO:0016444 GO:0016445 GO:0016447 GO:0019219 GO:0019222 GO:0019724 GO:0022402 GO:0023051 GO:0023052 GO:0023057 GO:0030888 GO:0030890 GO:0031323 GO:0031325 GO:0032501 GO:0032502 GO:0032943 GO:0032944 GO:0032946 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0035556 GO:0042100 GO:0042113 GO:0042127 GO:0042770 GO:0042771 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0045190 GO:0045191 GO:0045321 GO:0045830 GO:0045911 GO:0045935 GO:0046483 GO:0046649 GO:0046651 GO:0048302 GO:0048304 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048731 GO:0048856 GO:0050670 GO:0050671 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0050867 GO:0050871 GO:0050896 GO:0051052 GO:0051054 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051249 GO:0051251 GO:0051716 GO:0051726 GO:0060255 GO:0060548 GO:0065007 GO:0070661 GO:0070663 GO:0070665 GO:0071704 GO:0072331 GO:0072332 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0097190 GO:0097193 GO:1901360 GO:1901576 GO:1901796 GO:1901797 GO:1901987 GO:1901990 GO:1902165 GO:1902166 GO:1902229 GO:1902230 GO:1902253 GO:1902254 GO:1902531 GO:1902532 GO:2000026 GO:2001020 GO:2001021 GO:2001233 GO:2001234 GO:2001242 GO:2001243
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA Rad17

Protein Analysis

899

Amino Acids

100.35

Weight (kDa)

5.87

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_4 PF14570 133 - 181 4.5e-15 RING/Ubox like zinc-binding domain
Cellulose_synt PF03552 366 - 883 2.7e-228 Cellulose synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1028, 1106
Acc36I ACCTGC 1 cut(s) 453
Acc65I GGTACC 2 cut(s) 695, 2392
AccB1I GGYRCC 5 cut(s) 695, 1282, 1799, 2200, 2392
AccB7I CCANNNNNTGG 1 cut(s) 235
AccBSI CCGCTC 1 cut(s) 126
AciI CCGC 7 cut(s) 126, 260, 797, 1768, 2092, 2139, 2150
AclWI GGATC 8 cut(s) 772, 990, 1115, 1670, 1976, 1998, 2396, 2509
AcoI YGGCCR 4 cut(s) 91, 369, 1586, 1769
AcsI RAATTY 5 cut(s) 104, 659, 1641, 2287, 2363
AcuI CTGAAG 2 cut(s) 1809, 2448
AcvI CACGTG 3 cut(s) 317, 1563, 2399
AcyI GRCGYC 3 cut(s) 5, 2335, 2371
AdeI CACNNNGTG 1 cut(s) 2632
AfaI GTAC 7 cut(s) 697, 1594, 1751, 1818, 2066, 2394, 2456
AfiI CCNNNNNNNGG 7 cut(s) 235, 547, 734, 972, 1483, 1685, 2654
AflIII ACRYGT 5 cut(s) 162, 314, 627, 1101, 1752
AgeI ACCGGT 1 cut(s) 1520
AgsI TTSAA 9 cut(s) 13, 87, 304, 469, 1167, 1310, 1423, 1973, 2054
AjnI CCWGG 7 cut(s) 520, 601, 760, 971, 1481, 1771, 2377
AjuI GAANNNNNNNTTGG 2 cut(s) 1832, 1864
AleI CACNNNNGTG 2 cut(s) 245, 2544
Alw21I GWGCWC 3 cut(s) 322, 1231, 2041
Alw26I GTCTC 7 cut(s) 146, 680, 1114, 1511, 2171, 2440, 2472
AlwI GGATC 8 cut(s) 772, 990, 1115, 1670, 1976, 1998, 2396, 2509
AlwNI CAGNNNCTG 1 cut(s) 1259
AoxI GGCC 8 cut(s) 91, 369, 1557, 1586, 1769, 1844, 1893, 2644
ApeKI GCWGC 4 cut(s) 821, 824, 1259, 2269
ApoI RAATTY 5 cut(s) 104, 659, 1641, 2287, 2363
AseI ATTAAT 2 cut(s) 1008, 2607
AsiGI ACCGGT 1 cut(s) 1520
Asp718I GGTACC 2 cut(s) 695, 2392
AspS9I GGNCC 9 cut(s) 566, 605, 758, 1549, 1599, 1844, 1924, 2057, 2645
AsuC2I CCSGG 7 cut(s) 48, 221, 295, 1092, 1187, 1590, 2591
AsuHPI GGTGA 9 cut(s) 283, 364, 430, 668, 1640, 1663, 2180, 2563, 2669
AvaII GGWCC 7 cut(s) 566, 605, 758, 1549, 1599, 1924, 2057
BalI TGGCCA 1 cut(s) 93
BanI GGYRCC 5 cut(s) 695, 1282, 1799, 2200, 2392
BanII GRGCYC 1 cut(s) 2193
BbrPI CACGTG 3 cut(s) 317, 1563, 2399
BbsI GAAGAC 2 cut(s) 159, 2042
Bbv12I GWGCWC 3 cut(s) 322, 1231, 2041
BbvI GCAGC 4 cut(s) 833, 836, 1246, 2256
BceAI ACGGC 2 cut(s) 812, 2214
BciT130I CCWGG 7 cut(s) 522, 603, 762, 973, 1483, 1773, 2379
BciVI GTATCC 1 cut(s) 499
BclI TGATCA 3 cut(s) 664, 1780, 2347
BcnI CCSGG 7 cut(s) 48, 221, 295, 1092, 1187, 1590, 2591
BcoDI GTCTC 7 cut(s) 146, 680, 1114, 1511, 2171, 2440, 2472
BfaI CTAG 8 cut(s) 111, 1025, 1263, 1611, 1668, 2235, 2342, 2652
BfmI CTRYAG 3 cut(s) 1017, 1646, 2355
BfuAI ACCTGC 1 cut(s) 453
BfuI GTATCC 1 cut(s) 499
BglII AGATCT 1 cut(s) 1463
BisI GCNGC 8 cut(s) 797, 822, 825, 1260, 1769, 2139, 2151, 2270
BlpI GCTNAGC 1 cut(s) 55
BlsI GCNGC 8 cut(s) 798, 823, 826, 1261, 1770, 2140, 2152, 2271
Bme18I GGWCC 7 cut(s) 566, 605, 758, 1549, 1599, 1924, 2057
BmgT120I GGNCC 9 cut(s) 566, 605, 758, 1549, 1599, 1844, 1924, 2057, 2645
BmrI ACTGGG 1 cut(s) 2085
BmsI GCATC 8 cut(s) 387, 484, 763, 913, 1459, 1644, 2401, 2572
BmuI ACTGGG 1 cut(s) 2085
BpiI GAAGAC 2 cut(s) 159, 2042
Bpu10I CCTNAGC 2 cut(s) 2274, 2636
Bpu1102I GCTNAGC 1 cut(s) 55
BpuEI CTTGAG 2 cut(s) 130, 1643
BpuMI CCSGG 7 cut(s) 48, 221, 295, 1092, 1187, 1590, 2591
BsaAI YACGTR 4 cut(s) 317, 1563, 2068, 2399
BsaBI GATNNNNATC 2 cut(s) 510, 2262
BsaHI GRCGYC 3 cut(s) 5, 2335, 2371
BsaI GGTCTC 2 cut(s) 1511, 2472
BsaWI WCCGGW 1 cut(s) 1520
BsaXI ACNNNNNCTCC 4 cut(s) 2188, 2218, 2469, 2499
Bsc4I CCNNNNNNNGG 7 cut(s) 235, 547, 734, 972, 1483, 1685, 2654
Bse118I RCCGGY 1 cut(s) 1520
Bse1I ACTGG 3 cut(s) 1078, 2080, 2170
Bse3DI GCAATG 2 cut(s) 590, 1254
Bse8I GATNNNNATC 2 cut(s) 510, 2262
BseBI CCWGG 7 cut(s) 522, 603, 762, 973, 1483, 1773, 2379
BseJI GATNNNNATC 2 cut(s) 510, 2262
BseLI CCNNNNNNNGG 7 cut(s) 235, 547, 734, 972, 1483, 1685, 2654
BseMI GCAATG 2 cut(s) 590, 1254
BseMII CTCAG 6 cut(s) 69, 83, 339, 1976, 2187, 2313
BseNI ACTGG 3 cut(s) 1078, 2080, 2170
BseRI GAGGAG 4 cut(s) 278, 600, 1944, 1947
BseXI GCAGC 4 cut(s) 833, 836, 1246, 2256
BshFI GGCC 8 cut(s) 93, 371, 1559, 1588, 1771, 1846, 1895, 2646
BshNI GGYRCC 5 cut(s) 695, 1282, 1799, 2200, 2392
BshTI ACCGGT 1 cut(s) 1520
BsiHKAI GWGCWC 3 cut(s) 322, 1231, 2041
BslFI GGGAC 5 cut(s) 197, 563, 1535, 1589, 2092
BslI CCNNNNNNNGG 7 cut(s) 235, 547, 734, 972, 1483, 1685, 2654
BsmAI GTCTC 7 cut(s) 146, 680, 1114, 1511, 2171, 2440, 2472
BsmFI GGGAC 5 cut(s) 197, 563, 1535, 1589, 2092
BsmI GAATGC 3 cut(s) 1813, 2554, 2585
BsnI GGCC 8 cut(s) 93, 371, 1559, 1588, 1771, 1846, 1895, 2646
Bso31I GGTCTC 2 cut(s) 1511, 2472
Bsp1286I GDGCHC 4 cut(s) 322, 1231, 2041, 2193
Bsp1407I TGTACA 1 cut(s) 1749
Bsp1720I GCTNAGC 1 cut(s) 55
Bsp19I CCATGG 1 cut(s) 234
BspACI CCGC 7 cut(s) 126, 260, 797, 1768, 2092, 2139, 2150
BspANI GGCC 8 cut(s) 93, 371, 1559, 1588, 1771, 1846, 1895, 2646
BspCNI CTCAG 6 cut(s) 68, 82, 340, 1975, 2186, 2314
BspHI TCATGA 1 cut(s) 1917
BspMAI CTGCAG 1 cut(s) 2359
BspMI ACCTGC 1 cut(s) 453
BspPI GGATC 8 cut(s) 772, 990, 1115, 1670, 1976, 1998, 2396, 2509
BspQI GCTCTTC 1 cut(s) 1236
BspT107I GGYRCC 5 cut(s) 695, 1282, 1799, 2200, 2392
BspTNI GGTCTC 2 cut(s) 1511, 2472
BsrBI CCGCTC 1 cut(s) 126
BsrDI GCAATG 2 cut(s) 590, 1254
BsrFI RCCGGY 1 cut(s) 1520
BsrGI TGTACA 1 cut(s) 1749
BsrI ACTGG 3 cut(s) 1078, 2080, 2170
BssAI RCCGGY 1 cut(s) 1520
BssNI GRCGYC 3 cut(s) 5, 2335, 2371
BssT1I CCWWGG 2 cut(s) 234, 1812
Bst2UI CCWGG 7 cut(s) 522, 603, 762, 973, 1483, 1773, 2379
Bst4CI ACNGT 9 cut(s) 100, 202, 413, 737, 1868, 2014, 2422, 2534, 2546
Bst6I CTCTTC 4 cut(s) 293, 635, 1236, 2677
BstACI GRCGYC 3 cut(s) 5, 2335, 2371
BstAPI GCANNNNNTGC 2 cut(s) 483, 1259
BstAUI TGTACA 1 cut(s) 1749
BstBAI YACGTR 4 cut(s) 317, 1563, 2068, 2399
BstC8I GCNNGC 5 cut(s) 391, 402, 1204, 2037, 2644
BstDSI CCRYGG 2 cut(s) 234, 2545
BstEII GGTNACC 1 cut(s) 1628
BstENI CCTNNNNNAGG 1 cut(s) 2652
BstMAI GTCTC 7 cut(s) 146, 680, 1114, 1511, 2171, 2440, 2472
BstNI CCWGG 7 cut(s) 522, 603, 762, 973, 1483, 1773, 2379
BstNSI RCATGY 5 cut(s) 166, 631, 1105, 1206, 1756
BstPI GGTNACC 1 cut(s) 1628
BstSFI CTRYAG 3 cut(s) 1017, 1646, 2355
BstV1I GCAGC 4 cut(s) 833, 836, 1246, 2256
BstV2I GAAGAC 2 cut(s) 159, 2042
BstX2I RGATCY 1 cut(s) 1463
BstXI CCANNNNNNTGG 1 cut(s) 1567
BstYI RGATCY 1 cut(s) 1463
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 8 cut(s) 93, 371, 1559, 1588, 1771, 1846, 1895, 2646
BtgI CCRYGG 2 cut(s) 234, 2545
BtsI GCAGTG 1 cut(s) 1146
BtsIMutI CAGTG 3 cut(s) 1071, 1146, 2010
BveI ACCTGC 1 cut(s) 453
Cac8I GCNNGC 5 cut(s) 391, 402, 1204, 2037, 2644
CaiI CAGNNNCTG 1 cut(s) 1259
CciI TCATGA 1 cut(s) 1917
Cfr10I RCCGGY 1 cut(s) 1520
Cfr13I GGNCC 9 cut(s) 566, 605, 758, 1549, 1599, 1844, 1924, 2057, 2645
CseI GACGC 3 cut(s) 926, 2343, 2379
Csp6I GTAC 7 cut(s) 696, 1593, 1750, 1817, 2065, 2393, 2455
CspAI ACCGGT 1 cut(s) 1520
CspCI CAANNNNNGTGG 2 cut(s) 2049, 2084
CviQI GTAC 7 cut(s) 696, 1593, 1750, 1817, 2065, 2393, 2455
DraIII CACNNNGTG 1 cut(s) 2632
DrdI GACNNNNNNGTC 2 cut(s) 1028, 1106
DseDI GACNNNNNNGTC 2 cut(s) 1028, 1106
EaeI YGGCCR 4 cut(s) 91, 369, 1586, 1769
Eam1104I CTCTTC 4 cut(s) 293, 635, 1236, 2677
EarI CTCTTC 4 cut(s) 293, 635, 1236, 2677
EciI GGCGGA 1 cut(s) 275
Eco130I CCWWGG 2 cut(s) 234, 1812
Eco24I GRGCYC 1 cut(s) 2193
Eco31I GGTCTC 2 cut(s) 1511, 2472
Eco47I GGWCC 7 cut(s) 566, 605, 758, 1549, 1599, 1924, 2057
Eco57I CTGAAG 2 cut(s) 1809, 2448
Eco72I CACGTG 3 cut(s) 317, 1563, 2399
Eco91I GGTNACC 1 cut(s) 1628
EcoNI CCTNNNNNAGG 1 cut(s) 2652
EcoO109I RGGNCCY 1 cut(s) 1549
EcoO65I GGTNACC 1 cut(s) 1628
EcoRI GAATTC 1 cut(s) 1641
EcoRII CCWGG 7 cut(s) 520, 601, 760, 971, 1481, 1771, 2377
EcoT14I CCWWGG 2 cut(s) 234, 1812
EcoT22I ATGCAT 2 cut(s) 718, 2587
EcoT38I GRGCYC 1 cut(s) 2193
ErhI CCWWGG 2 cut(s) 234, 1812
FaqI GGGAC 5 cut(s) 197, 563, 1535, 1589, 2092
FbaI TGATCA 3 cut(s) 664, 1780, 2347
Fnu4HI GCNGC 8 cut(s) 797, 822, 825, 1260, 1769, 2139, 2151, 2270
FriOI GRGCYC 1 cut(s) 2193
Fsp4HI GCNGC 8 cut(s) 797, 822, 825, 1260, 1769, 2139, 2151, 2270
FspBI CTAG 8 cut(s) 111, 1025, 1263, 1611, 1668, 2235, 2342, 2652
GluI GCNGC 8 cut(s) 797, 822, 825, 1260, 1769, 2139, 2151, 2270
HaeIII GGCC 8 cut(s) 93, 371, 1559, 1588, 1771, 1846, 1895, 2646
HgaI GACGC 3 cut(s) 926, 2343, 2379
Hin1I GRCGYC 3 cut(s) 5, 2335, 2371
HincII GTYRAC 1 cut(s) 1348
HindII GTYRAC 1 cut(s) 1348
HindIII AAGCTT 1 cut(s) 786
HinfI GANTC 9 cut(s) 300, 344, 438, 864, 1448, 1734, 2171, 2306, 2622
HphI GGTGA 9 cut(s) 283, 364, 430, 668, 1640, 1663, 2180, 2563, 2669
Hpy166II GTNNAC 3 cut(s) 1348, 1750, 2240
Hpy8I GTNNAC 3 cut(s) 1348, 1750, 2240
HpyCH4III ACNGT 9 cut(s) 100, 202, 413, 737, 1868, 2014, 2422, 2534, 2546
HpyCH4IV ACGT 4 cut(s) 316, 1562, 2067, 2398
HpySE526I ACGT 4 cut(s) 316, 1562, 2067, 2398
Hsp92I GRCGYC 3 cut(s) 5, 2335, 2371
KpnI GGTACC 2 cut(s) 699, 2396
Ksp22I TGATCA 3 cut(s) 664, 1780, 2347
LguI GCTCTTC 1 cut(s) 1236
LmnI GCTCC 7 cut(s) 265, 374, 532, 587, 1132, 1537, 2605
Lsp1109I GCAGC 4 cut(s) 833, 836, 1246, 2256
LweI GCATC 8 cut(s) 387, 484, 763, 913, 1459, 1644, 2401, 2572
MaeI CTAG 8 cut(s) 111, 1025, 1263, 1611, 1668, 2235, 2342, 2652
MaeII ACGT 4 cut(s) 316, 1562, 2067, 2398
MbiI CCGCTC 1 cut(s) 126
MfeI CAATTG 3 cut(s) 596, 816, 942
MflI RGATCY 1 cut(s) 1463
MhlI GDGCHC 4 cut(s) 322, 1231, 2041, 2193
MlsI TGGCCA 1 cut(s) 93
MluNI TGGCCA 1 cut(s) 93
MlyI GAGTC 5 cut(s) 447, 1442, 1728, 2165, 2616
MmeI TCCRAC 1 cut(s) 2471
Mox20I TGGCCA 1 cut(s) 93
Mph1103I ATGCAT 2 cut(s) 718, 2587
MscI TGGCCA 1 cut(s) 93
MseI TTAA 7 cut(s) 720, 792, 1008, 1340, 1434, 1502, 2607
MslI CAYNNNNRTG 5 cut(s) 245, 632, 1100, 1565, 2544
Msp20I TGGCCA 1 cut(s) 93
MspA1I CMGCKG 1 cut(s) 59
MunI CAATTG 3 cut(s) 596, 816, 942
Mva1269I GAATGC 3 cut(s) 1813, 2554, 2585
MvaI CCWGG 7 cut(s) 522, 603, 762, 973, 1483, 1773, 2379
NciI CCSGG 7 cut(s) 48, 221, 295, 1092, 1187, 1590, 2591
NcoI CCATGG 1 cut(s) 234
NmuCI GTSAC 9 cut(s) 310, 352, 407, 1613, 1628, 1868, 2168, 2506, 2521
NsiI ATGCAT 2 cut(s) 718, 2587
NspI RCATGY 5 cut(s) 166, 631, 1105, 1206, 1756
OliI CACNNNNGTG 2 cut(s) 245, 2544
PaeI GCATGC 1 cut(s) 1206
PagI TCATGA 1 cut(s) 1917
PciI ACATGT 4 cut(s) 162, 627, 1101, 1752
PciSI GCTCTTC 1 cut(s) 1236
PctI GAATGC 3 cut(s) 1813, 2554, 2585
PfeI GAWTC 4 cut(s) 300, 344, 864, 2306
PflFI GACNNNGTC 1 cut(s) 2132
PflMI CCANNNNNTGG 1 cut(s) 235
PfoI TCCNGGA 1 cut(s) 971
PinAI ACCGGT 1 cut(s) 1520
PkrI GCNGC 8 cut(s) 798, 823, 826, 1261, 1770, 2140, 2152, 2271
PleI GAGTC 5 cut(s) 446, 1442, 1728, 2165, 2616
PmaCI CACGTG 3 cut(s) 317, 1563, 2399
PmlI CACGTG 3 cut(s) 317, 1563, 2399
PpsI GAGTC 5 cut(s) 446, 1442, 1728, 2165, 2616
Ppu21I YACGTR 4 cut(s) 317, 1563, 2068, 2399
PpuMI RGGWCCY 1 cut(s) 1549
PscI ACATGT 4 cut(s) 162, 627, 1101, 1752
PshBI ATTAAT 2 cut(s) 1008, 2607
Psp5II RGGWCCY 1 cut(s) 1549
Psp6I CCWGG 7 cut(s) 520, 601, 760, 971, 1481, 1771, 2377
PspCI CACGTG 3 cut(s) 317, 1563, 2399
PspEI GGTNACC 1 cut(s) 1628
PspGI CCWGG 7 cut(s) 520, 601, 760, 971, 1481, 1771, 2377
PspPI GGNCC 9 cut(s) 566, 605, 758, 1549, 1599, 1844, 1924, 2057, 2645
PspPPI RGGWCCY 1 cut(s) 1549
PsrI GAACNNNNNNTAC 2 cut(s) 1403, 1435
PstI CTGCAG 1 cut(s) 2359
PstNI CAGNNNCTG 1 cut(s) 1259
PsuI RGATCY 1 cut(s) 1463
PsyI GACNNNGTC 1 cut(s) 2132
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 7 cut(s) 697, 1594, 1751, 1818, 2066, 2394, 2456
RsaNI GTAC 7 cut(s) 696, 1593, 1750, 1817, 2065, 2393, 2455
RseI CAYNNNNRTG 5 cut(s) 245, 632, 1100, 1565, 2544
SapI GCTCTTC 1 cut(s) 1236
SaqAI TTAA 7 cut(s) 720, 792, 1008, 1340, 1434, 1502, 2607
SatI GCNGC 8 cut(s) 797, 822, 825, 1260, 1769, 2139, 2151, 2270
Sau96I GGNCC 9 cut(s) 566, 605, 758, 1549, 1599, 1844, 1924, 2057, 2645
SchI GAGTC 5 cut(s) 447, 1442, 1728, 2165, 2616
SduI GDGCHC 4 cut(s) 322, 1231, 2041, 2193
SfaNI GCATC 8 cut(s) 387, 484, 763, 913, 1459, 1644, 2401, 2572
SfcI CTRYAG 3 cut(s) 1017, 1646, 2355
SinI GGWCC 7 cut(s) 566, 605, 758, 1549, 1599, 1924, 2057
SmiMI CAYNNNNRTG 5 cut(s) 245, 632, 1100, 1565, 2544
SmlI CTYRAG 2 cut(s) 145, 1658
SmoI CTYRAG 2 cut(s) 145, 1658
SphI GCATGC 1 cut(s) 1206
SsiI CCGC 7 cut(s) 126, 260, 797, 1768, 2092, 2139, 2150
SspMI CTAG 8 cut(s) 111, 1025, 1263, 1611, 1668, 2235, 2342, 2652
StyI CCWWGG 2 cut(s) 234, 1812
TaaI ACNGT 9 cut(s) 100, 202, 413, 737, 1868, 2014, 2422, 2534, 2546
TaiI ACGT 4 cut(s) 319, 1565, 2070, 2401
TaqI TCGA 7 cut(s) 122, 441, 862, 1863, 2022, 2244, 2620
TaqII GACCGA 2 cut(s) 1941, 2496
TatI WGTACW 1 cut(s) 1749
TauI GCSGC 4 cut(s) 799, 1771, 2141, 2153
TfiI GAWTC 4 cut(s) 300, 344, 864, 2306
Tru1I TTAA 7 cut(s) 720, 792, 1008, 1340, 1434, 1502, 2607
Tru9I TTAA 7 cut(s) 720, 792, 1008, 1340, 1434, 1502, 2607
TscAI CASTG 3 cut(s) 1078, 1153, 2017
TseFI GTSAC 9 cut(s) 310, 352, 407, 1613, 1628, 1868, 2168, 2506, 2521
TseI GCWGC 4 cut(s) 821, 824, 1259, 2269
Tsp45I GTSAC 9 cut(s) 310, 352, 407, 1613, 1628, 1868, 2168, 2506, 2521
TspGWI ACGGA 2 cut(s) 1236, 1591
TspRI CASTG 3 cut(s) 1078, 1153, 2017
Tth111I GACNNNGTC 1 cut(s) 2132
Van91I CCANNNNNTGG 1 cut(s) 235
VpaK11BI GGWCC 7 cut(s) 566, 605, 758, 1549, 1599, 1924, 2057
VspI ATTAAT 2 cut(s) 1008, 2607
XagI CCTNNNNNAGG 1 cut(s) 2652
XapI RAATTY 5 cut(s) 104, 659, 1641, 2287, 2363
XceI RCATGY 5 cut(s) 166, 631, 1105, 1206, 1756
XcmI CCANNNNNNNNNTGG 2 cut(s) 1560, 1810
XspI CTAG 8 cut(s) 111, 1025, 1263, 1611, 1668, 2235, 2342, 2652
Zsp2I ATGCAT 2 cut(s) 718, 2587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.