pycom15g31350
ERF Family

superfamily. Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006838
Physical Location & Seq
Reverse (-)
29770353 .. 29780421
10069 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g31350.15

Sequence Viewer

Length: 3576 bp
ATGGCCGATTCAGCTCAGAACGAGTCGCCGACGACGCCTAAACGGCAAGTTCGCCGGAAACTGGTCCAGACCACATTGTTCCCTCTCAAACCCCAGCAACAGCTCGAGTTAAACGGCGATCTGAAGGCCGCGAGAGGTAATGCTGGTGACGACGACTGCGGCGACGACGAGGACTTCTGTGGGAGTCAAAGTAAAACGAAGAGGAAGTCAAGGGGAAAGAAGACGCCTCCCATTAAAGCCCCGAAGAAGGACGGTAAGCGATCCACGAATAGTACGCCGAAGAAGAAGATTAATGGAACTGAAGTGGAGAGTGAAGATGCGCCTCGAGTCGTCACTGATTTGAGGCTAGAGGCTAGATTGAAAGCTGAGGAAACGTCACGGATATTTTCAGGAATGTTTTCTGGAAAGCAAATACATCCATTTTTCTCAATTTGTAAAGCTGGAAAAAGAAAGCGAGAGGCAACTGAAGTTGATGTCGATTTGAGCTACGTTGGGAGGAAGGATAAAGAGATCACTAGTGGTCCGATTCATGTGTTTGAAAGGACTCAGGATGATGCTGTGTTCCTTGATTGGAGAAACTGGACATTTTGTGAGGACACGTTTATGAAAAGTGGTCCAGACATGGAATGCGCGTCCTCATCAATTTTTGAAGGCTCTGTCGAGTGCTTAAATTTCGACAAGCTTCCCATTGTTTTCCGACCCTGCAAATTATCGACTTTTCAGAATGTGGTATCCTTAGATCAGCATTGTATTCAACAAGAATTCGCTCATGATATATCACCAACAGTCCCTGGCTTCTTAGTCGATGAGCAGTTGATGCACTATCAGCAGTCAACGGAAGCAGAGGATAATGAGATGCTTAAAGTTGGTTTGCTTTCTCAGCATACCACTTATATAAAGAAGTCAGATATCGAGCAGCAGAAAATTCTCGAGGAAAGGTTGATGTCAAACTATTCCAGTTGTGGTAATCAGCCCACAAATAGCTTATGGACATATAAGTACCGGCCAATGAAGGCTATTGATGTATGTGGTAACCATGAATCTGTGAATTTTTTGAGCGAATGGCTTCGTCTTTGGTATGAAAGAGATTTTCGAGCCAACAAAGATCTCACTAGGTGTTCTCAAAGTGACTCTGATTTAGAAGGTGAAGATGAAGAGGCAAGCATGAAGAAGAATAATGTTCTCTTAGTTACAGGGCCAACTGGGAGTGGAAAATCTGCAGCTATTTATGCTTGTGCCCAAGAGCAAGGTTTTAAGGTTTTGGAGCTCAGTGCATCAGAATGTCGGAGTGGGGCTCTTGTAAAACAGAGGTTTGGAGAGGCTTTGAAATCTCACAATTTTAGAAGATCAGTGGGAAATCCTGAGGGTTCACAGAACAAGTCTACAGTGAAATCCCCGTTTGTTGATGCAAATGGTATGACAGACCATGAGATGGATGATGATGTTATTGAATTGATAGCCGTATCAGATGAAGATTCTCATGAGGCAACTGAAGCCTCTGTAAAATCCGTCTCTAAGGAGGATTATAGCGAAGTTAAACATCTTATACTTTTTGACGATGTGGACATCACTTTTCTTGAAGATCGTGGTTTCATTGCAGCAATTCAGCAGATAGCTAAAACAGCCAAGGGGCCCATAATACTGACCAGCAATAGTGACAATCCTGTCCTGCCAGACAGTTTTGACAGATTACAAGTGCGTTTCACGTTGCCATCACAAAAGGAGCTGCATTCTCTTGCATATATGGTTTCTGCTTTGGAGAGAGCCAACATACAACCTTTGCTGCTTGAGCGGCTTATTGAGTGTTGCCGGGGAGATATTCGGAAGATCTTTATGCATCTTCAGTTCTGGTGCCAGGGTGGAAGATTTAGAAAAGATACCAAAATGCGAGAGTGGTATGGTTCCCTGCTATTTGATGTTGAGGCTGGCCATCTGATGCTGCCAAATATGTTACCCTGGGATTTACCTTCTCAGTTATCTGATGTAGTAGAGAAAGAGATAACAAAGGCATTATACATGATGGAAGAAAGTCCAAGTTCAATGGACGTAATTGAAGAAAATCTTCACGAGATAGAAGTGCAATACAGTTTGGACATGCCTTGTAATGGAATGGAAAGTTTAGAGGCCAAGAAGGTAGAAATGCTGAGCAGGAATGGCTCTGTTCATGACTGCTATCACTATACGGCTCAAACTGATACTGCTTCTGAGTTTTCCAACGATTTAGGTACCCCATTTTCCTCATGTCGGCGTCATGTTCGGAATATGCAAGCTGTTGTAATGTCTGATTCTGAAGACGAGTTTATGACCAATGGATATCCTATAGTTACAGATAACGCTAACAATGAAGTCCTTGGTATTAACCCATTGCCTGAAGAGCTACTTATTTTTGGAGCGGCTAATATAGATGGACCTTGTGAATGTTCAGAACTTGCAGATGGGGTGCACATAAGCGAGACTTGCAACTCAGTTGATATATCATGTGTACCGGAATCGTCTTTTGTTCCTGAAACTGAAATTGACAATATGCCAGAATTATCTTCTCAAACAGTGTCTTCTGACCATTTTGCCAACGCCATGAAAAGAGTATCTTTAGACGATGAATTACATGGAGCCAACAATCTTAATAAACAAATATTCGTACAAGGTAACTCTGCTAAGTGGGGAAGTGGCTGTGCTACAATTGCAGAGTATTCTCACAAGGAGTTTGAGAATCAAAAGGAACATGCGGAGACTGTTGCAAGAGCTTATCAGTTGATGGATGAGTGTAGCCGCATGGATTTTAGTAAGGGCTCGCAATTTATACAGGGGCAAAAGTCCTCAGCGGTGACAGATTTAGTGCAAGATTCATGGGACAAACTTCGTGGCTCCCGCATTGATCTTAGGCAGTATATTGCATCCGAACAACCCAATGCCTCTCAGATTGTCATGCTTGCTGATCGAATGAGCAATTTGATCTCAGAAACTGACATGTTGTTTTCTAAATGCCAATCATTGATGAATGATTCTTTCGAACTATCAATGATCCCCTTGGAGGAGTCAGATGCATACAGTTGGTGTGACGAGAGATTATTGTTGGCGTCCACAATTGCGCAGCATGGGTTTTGCTTCTATGCAAAATGCATTTCTTCTGTAGGATCAAAAGAAGGTTGTGTAGGGAGGGTGGATTTGGCTGGGAAAATGCTGGCTAACACAGCCAGTACGATGGCATTCAGTAAGTTAATCGGGAAGGGTATGAGAGCAAGTAAGGCTTCATCTGCCGAAAGAAATTCAGAGACATCCTTGCCAAATGCGACAAGTGAAATCCAATCACGTGTATTTGATGTAATTCGGTCAATTGTACCTTCAAGAACGTACTCAAATTTGAGAGGTGGTGCACAGCATGAGTATCTTTCTTCTCTGCGCCACATTTCAAGATCAGAAGCTTCTCGGTTATCAGAAGGAGCTGAGAAGACCACCAGAAGTAGGAGGAGGGTTGCTCCGCACTACTTAAGTAGGGGTGCACTGATGTTATCTCCTGAACATATATCGTTGCTGGATCAGTATGGCTCATACGGGAAAACCTCCTCTTCATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0001775 GO:0002200 GO:0002204 GO:0002208 GO:0002250 GO:0002252 GO:0002263 GO:0002285 GO:0002312 GO:0002366 GO:0002376 GO:0002377 GO:0002381 GO:0002440 GO:0002443 GO:0002449 GO:0002460 GO:0002520 GO:0002562 GO:0002637 GO:0002639 GO:0002682 GO:0002684 GO:0002694 GO:0002696 GO:0002697 GO:0002699 GO:0002700 GO:0002702 GO:0002703 GO:0002705 GO:0002706 GO:0002708 GO:0002712 GO:0002714 GO:0002819 GO:0002821 GO:0002822 GO:0002824 GO:0002889 GO:0002891 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006310 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0006955 GO:0006974 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0008283 GO:0008284 GO:0008630 GO:0009058 GO:0009059 GO:0009893 GO:0009966 GO:0009968 GO:0009987 GO:0010564 GO:0010604 GO:0010646 GO:0010648 GO:0010941 GO:0012501 GO:0016064 GO:0016444 GO:0016445 GO:0016447 GO:0019219 GO:0019222 GO:0019724 GO:0022402 GO:0023051 GO:0023052 GO:0023057 GO:0030888 GO:0030890 GO:0031323 GO:0031325 GO:0032501 GO:0032502 GO:0032943 GO:0032944 GO:0032946 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0035556 GO:0042100 GO:0042113 GO:0042127 GO:0042770 GO:0042771 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0045190 GO:0045191 GO:0045321 GO:0045830 GO:0045911 GO:0045935 GO:0046483 GO:0046649 GO:0046651 GO:0048302 GO:0048304 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048731 GO:0048856 GO:0050670 GO:0050671 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0050867 GO:0050871 GO:0050896 GO:0051052 GO:0051054 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051249 GO:0051251 GO:0051716 GO:0051726 GO:0060255 GO:0060548 GO:0065007 GO:0070661 GO:0070663 GO:0070665 GO:0071704 GO:0072331 GO:0072332 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0097190 GO:0097193 GO:1901360 GO:1901576 GO:1901796 GO:1901797 GO:1901987 GO:1901990 GO:1902165 GO:1902166 GO:1902229 GO:1902230 GO:1902253 GO:1902254 GO:1902531 GO:1902532 GO:2000026 GO:2001020 GO:2001021 GO:2001233 GO:2001234 GO:2001242 GO:2001243
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA Rad17

Protein Analysis

1192

Amino Acids

133.36

Weight (kDa)

5.24

Isoelectric Point (pI)

51.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1664
Acc16I TGCGCA 1 cut(s) 3090
Acc65I GGTACC 1 cut(s) 2225
AccB1I GGYRCC 2 cut(s) 1851, 2225
AccB7I CCANNNNNTGG 1 cut(s) 1432
AccBSI CCGCTC 2 cut(s) 1792, 2393
AccI GTMKAC 1 cut(s) 1382
AccII CGCG 2 cut(s) 131, 632
AciI CCGC 9 cut(s) 129, 159, 1792, 2393, 2726, 2770, 2822, 2869, 3479
AclWI GGATC 4 cut(s) 255, 3016, 3142, 3543
AcoI YGGCCR 3 cut(s) 3, 1004, 1927
AcsI RAATTY 6 cut(s) 670, 761, 924, 1048, 3265, 3358
AcuI CTGAAG 7 cut(s) 143, 321, 486, 1512, 1826, 2310, 2391
AcvI CACGTG 1 cut(s) 3311
AcyI GRCGYC 4 cut(s) 35, 224, 2248, 3077
AdeI CACNNNGTG 1 cut(s) 1116
AfaI GTAC 8 cut(s) 274, 1001, 2227, 2484, 2640, 3199, 3339, 3353
AfiI CCNNNNNNNGG 7 cut(s) 61, 247, 1432, 2105, 2244, 3031, 3462
AflII CTTAAG 1 cut(s) 3487
AflIII ACRYGT 3 cut(s) 597, 2967, 3310
AhlI ACTAGT 1 cut(s) 515
AjnI CCWGG 3 cut(s) 790, 1854, 1955
Alw21I GWGCWC 4 cut(s) 1269, 2445, 3376, 3502
Alw26I GTCTC 4 cut(s) 1516, 2447, 2723, 3266
Alw44I GTGCAC 3 cut(s) 2441, 3372, 3498
AlwI GGATC 4 cut(s) 255, 3016, 3142, 3543
AlwNI CAGNNNCTG 2 cut(s) 791, 2963
Ama87I CYCGRG 3 cut(s) 104, 324, 929
AoxI GGCC 7 cut(s) 3, 126, 1004, 1196, 1631, 1927, 2124
ApaI GGGCCC 1 cut(s) 1635
ApaLI GTGCAC 3 cut(s) 2441, 3372, 3498
ApeKI GCWGC 7 cut(s) 916, 1220, 1598, 1726, 1783, 1939, 3091
ApoI RAATTY 6 cut(s) 670, 761, 924, 1048, 3265, 3358
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AseI ATTAAT 1 cut(s) 291
Asp700I GAANNNNTTC 3 cut(s) 397, 1065, 2061
Asp718I GGTACC 1 cut(s) 2225
AspLEI GCGC 4 cut(s) 322, 632, 3091, 3402
AspS9I GGNCC 7 cut(s) 64, 521, 614, 1196, 1631, 1632, 2408
AsuC2I CCSGG 1 cut(s) 1811
AsuHPI GGTGA 4 cut(s) 158, 771, 1157, 2836
AsuII TTCGAA 1 cut(s) 3009
AvaI CYCGRG 3 cut(s) 104, 324, 929
AvaII GGWCC 4 cut(s) 64, 521, 614, 2408
AxyI CCTNAGG 1 cut(s) 1362
BaeGI GKGCMC 5 cut(s) 1240, 1635, 2445, 3376, 3502
BalI TGGCCA 1 cut(s) 1929
BanI GGYRCC 2 cut(s) 1851, 2225
BanII GRGCYC 4 cut(s) 1269, 1297, 1635, 2792
BauI CACGAG 1 cut(s) 2066
BbrPI CACGTG 1 cut(s) 3311
BbsI GAAGAC 4 cut(s) 227, 2298, 2544, 3455
Bbv12I GWGCWC 4 cut(s) 1269, 2445, 3376, 3502
BbvCI CCTCAGC 2 cut(s) 366, 2818
BbvI GCAGC 7 cut(s) 928, 1232, 1610, 1713, 1770, 1926, 3103
BccI CCATC 8 cut(s) 1426, 1720, 1938, 2014, 2399, 2429, 2749, 3196
BceAI ACGGC 4 cut(s) 59, 130, 1445, 2199
BciT130I CCWGG 3 cut(s) 792, 1856, 1957
BciVI GTATCC 1 cut(s) 742
BcnI CCSGG 1 cut(s) 1811
BcoDI GTCTC 4 cut(s) 1516, 2447, 2723, 3266
BcuI ACTAGT 1 cut(s) 515
BfaI CTAG 4 cut(s) 347, 354, 516, 1113
BfmI CTRYAG 4 cut(s) 1218, 1383, 2319, 3129
BfrI CTTAAG 1 cut(s) 3487
BfuI GTATCC 1 cut(s) 742
BglI GCCNNNNNGGC 1 cut(s) 43
BglII AGATCT 2 cut(s) 1105, 1827
BlpI GCTNAGC 1 cut(s) 2144
Bme1390I CCNGG 4 cut(s) 792, 1811, 1856, 1957
Bme18I GGWCC 4 cut(s) 64, 521, 614, 2408
BmeT110I CYCGRG 3 cut(s) 104, 324, 929
BmgT120I GGNCC 7 cut(s) 64, 521, 614, 1196, 1631, 1632, 2408
BmiI GGNNCC 7 cut(s) 1632, 1633, 1853, 1903, 2227, 2611, 2866
BmrFI CCNGG 4 cut(s) 792, 1811, 1856, 1957
BmrI ACTGGG 1 cut(s) 1212
BmuI ACTGGG 1 cut(s) 1212
BpiI GAAGAC 4 cut(s) 227, 2298, 2544, 3455
BplI GAGNNNNNCTC 4 cut(s) 1279, 1311, 3460, 3492
Bpu10I CCTNAGC 2 cut(s) 366, 2818
Bpu1102I GCTNAGC 1 cut(s) 2144
Bpu14I TTCGAA 1 cut(s) 3009
BpuEI CTTGAG 1 cut(s) 1808
BpuMI CCSGG 1 cut(s) 1811
BsaAI YACGTR 1 cut(s) 3311
BsaHI GRCGYC 4 cut(s) 35, 224, 2248, 3077
BsaJI CCNNGG 8 cut(s) 790, 1626, 1810, 1855, 1955, 1956, 2350, 3027
BsaWI WCCGGW 1 cut(s) 2485
BsaXI ACNNNNNCTCC 2 cut(s) 175, 205
Bsc4I CCNNNNNNNGG 7 cut(s) 61, 247, 1432, 2105, 2244, 3031, 3462
Bse118I RCCGGY 1 cut(s) 1002
Bse1I ACTGG 5 cut(s) 66, 584, 957, 1207, 3195
Bse21I CCTNAGG 1 cut(s) 1362
Bse3DI GCAATG 2 cut(s) 1593, 2363
BseBI CCWGG 3 cut(s) 792, 1856, 1957
BseDI CCNNGG 8 cut(s) 790, 1626, 1810, 1855, 1955, 1956, 2350, 3027
BseGI GGATG 6 cut(s) 415, 556, 1441, 2764, 2894, 3275
BseLI CCNNNNNNNGG 7 cut(s) 61, 247, 1432, 2105, 2244, 3031, 3462
BseMI GCAATG 2 cut(s) 1593, 2363
BseNI ACTGG 5 cut(s) 66, 584, 957, 1207, 3195
BseRI GAGGAG 3 cut(s) 3047, 3481, 3553
BseSI GKGCMC 5 cut(s) 1240, 1635, 2445, 3376, 3502
BseXI GCAGC 7 cut(s) 928, 1232, 1610, 1713, 1770, 1926, 3103
BseYI CCCAGC 2 cut(s) 93, 3170
Bsh1236I CGCG 2 cut(s) 131, 632
BshFI GGCC 7 cut(s) 5, 128, 1006, 1198, 1633, 1929, 2126
BshNI GGYRCC 2 cut(s) 1851, 2225
BsiHKAI GWGCWC 4 cut(s) 1269, 2445, 3376, 3502
BsiHKCI CYCGRG 3 cut(s) 104, 324, 929
BsiSI CCGG 4 cut(s) 55, 1003, 1810, 2486
BslFI GGGAC 2 cut(s) 773, 2864
BslI CCNNNNNNNGG 7 cut(s) 61, 247, 1432, 2105, 2244, 3031, 3462
BsmAI GTCTC 4 cut(s) 1516, 2447, 2723, 3266
BsmBI CGTCTC 1 cut(s) 1516
BsmFI GGGAC 2 cut(s) 773, 2864
BsmI GAATGC 3 cut(s) 632, 1729, 3206
BsnI GGCC 7 cut(s) 5, 128, 1006, 1198, 1633, 1929, 2126
BsoBI CYCGRG 3 cut(s) 104, 324, 929
Bsp119I TTCGAA 1 cut(s) 3009
Bsp120I GGGCCC 1 cut(s) 1631
Bsp1286I GDGCHC 8 cut(s) 1240, 1269, 1297, 1635, 2445, 2792, 3376, 3502
Bsp1720I GCTNAGC 1 cut(s) 2144
BspACI CCGC 9 cut(s) 129, 159, 1792, 2393, 2726, 2770, 2822, 2869, 3479
BspANI GGCC 7 cut(s) 5, 128, 1006, 1198, 1633, 1929, 2126
BspFNI CGCG 2 cut(s) 131, 632
BspHI TCATGA 3 cut(s) 769, 1480, 2164
BspLI GGNNCC 7 cut(s) 1632, 1633, 1853, 1903, 2227, 2611, 2866
BspMAI CTGCAG 1 cut(s) 1222
BspPI GGATC 4 cut(s) 255, 3016, 3142, 3543
BspQI GCTCTTC 1 cut(s) 2367
BspT104I TTCGAA 1 cut(s) 3009
BspT107I GGYRCC 2 cut(s) 1851, 2225
BspTI CTTAAG 1 cut(s) 3487
BsrBI CCGCTC 2 cut(s) 1792, 2393
BsrDI GCAATG 2 cut(s) 1593, 2363
BsrFI RCCGGY 1 cut(s) 1002
BsrI ACTGG 5 cut(s) 66, 584, 957, 1207, 3195
BssAI RCCGGY 1 cut(s) 1002
BssECI CCNNGG 8 cut(s) 790, 1626, 1810, 1855, 1955, 1956, 2350, 3027
BssNI GRCGYC 4 cut(s) 35, 224, 2248, 3077
BssSI CACGAG 1 cut(s) 2066
BssT1I CCWWGG 3 cut(s) 1626, 2350, 3027
Bst2BI CACGAG 1 cut(s) 2066
Bst2UI CCWGG 3 cut(s) 792, 1856, 1957
Bst4CI ACNGT 8 cut(s) 254, 787, 1387, 1679, 2087, 2548, 2734, 3050
Bst6I CTCTTC 4 cut(s) 194, 1149, 2367, 3571
BstACI GRCGYC 4 cut(s) 35, 224, 2248, 3077
BstAFI CTTAAG 1 cut(s) 3487
BstAPI GCANNNNNTGC 1 cut(s) 817
BstBAI YACGTR 1 cut(s) 3311
BstBI TTCGAA 1 cut(s) 3009
BstC8I GCNNGC 6 cut(s) 1162, 1927, 2268, 2792, 2931, 3183
BstEII GGTNACC 1 cut(s) 1031
BstF5I GGATG 6 cut(s) 415, 556, 1441, 2764, 2894, 3275
BstFNI CGCG 2 cut(s) 131, 632
BstHHI GCGC 4 cut(s) 322, 632, 3091, 3402
BstMAI GTCTC 4 cut(s) 1516, 2447, 2723, 3266
BstNI CCWGG 3 cut(s) 792, 1856, 1957
BstNSI RCATGY 3 cut(s) 2098, 2726, 2971
BstPI GGTNACC 1 cut(s) 1031
BstSCI CCNGG 4 cut(s) 790, 1809, 1854, 1955
BstSFI CTRYAG 4 cut(s) 1218, 1383, 2319, 3129
BstSLI GKGCMC 5 cut(s) 1240, 1635, 2445, 3376, 3502
BstUI CGCG 2 cut(s) 131, 632
BstV1I GCAGC 7 cut(s) 928, 1232, 1610, 1713, 1770, 1926, 3103
BstV2I GAAGAC 4 cut(s) 227, 2298, 2544, 3455
BstX2I RGATCY 2 cut(s) 1105, 1827
BstXI CCANNNNNNTGG 1 cut(s) 3202
BstYI RGATCY 2 cut(s) 1105, 1827
Bsu36I CCTNAGG 1 cut(s) 1362
BsuI GTATCC 1 cut(s) 742
BsuRI GGCC 7 cut(s) 5, 128, 1006, 1198, 1633, 1929, 2126
BtsCI GGATG 6 cut(s) 415, 556, 1441, 2764, 2894, 3275
BtsIMutI CAGTG 6 cut(s) 333, 1276, 1356, 1392, 2553, 3500
Cac8I GCNNGC 6 cut(s) 1162, 1927, 2268, 2792, 2931, 3183
CaiI CAGNNNCTG 2 cut(s) 791, 2963
CciI TCATGA 3 cut(s) 769, 1480, 2164
CfoI GCGC 4 cut(s) 322, 632, 3091, 3402
Cfr10I RCCGGY 1 cut(s) 1002
Cfr13I GGNCC 7 cut(s) 64, 521, 614, 1196, 1631, 1632, 2408
CseI GACGC 5 cut(s) 43, 232, 621, 2237, 3066
Csp6I GTAC 8 cut(s) 273, 1000, 2226, 2483, 2639, 3198, 3338, 3352
CspCI CAANNNNNGTGG 2 cut(s) 2842, 2877
CviQI GTAC 8 cut(s) 273, 1000, 2226, 2483, 2639, 3198, 3338, 3352
DraIII CACNNNGTG 1 cut(s) 1116
DrdI GACNNNNNNGTC 1 cut(s) 1664
DseDI GACNNNNNNGTC 1 cut(s) 1664
EaeI YGGCCR 3 cut(s) 3, 1004, 1927
Eam1104I CTCTTC 4 cut(s) 194, 1149, 2367, 3571
EarI CTCTTC 4 cut(s) 194, 1149, 2367, 3571
Ecl136II GAGCTC 1 cut(s) 1267
Eco130I CCWWGG 3 cut(s) 1626, 2350, 3027
Eco24I GRGCYC 4 cut(s) 1269, 1297, 1635, 2792
Eco32I GATATC 2 cut(s) 910, 2315
Eco47I GGWCC 4 cut(s) 64, 521, 614, 2408
Eco53kI GAGCTC 1 cut(s) 1267
Eco57I CTGAAG 7 cut(s) 143, 321, 486, 1512, 1826, 2310, 2391
Eco72I CACGTG 1 cut(s) 3311
Eco81I CCTNAGG 1 cut(s) 1362
Eco88I CYCGRG 3 cut(s) 104, 324, 929
Eco91I GGTNACC 1 cut(s) 1031
EcoICRI GAGCTC 1 cut(s) 1267
EcoO109I RGGNCCY 1 cut(s) 1631
EcoO65I GGTNACC 1 cut(s) 1031
EcoRI GAATTC 1 cut(s) 761
EcoRII CCWGG 3 cut(s) 790, 1854, 1955
EcoRV GATATC 2 cut(s) 910, 2315
EcoT14I CCWWGG 3 cut(s) 1626, 2350, 3027
EcoT22I ATGCAT 3 cut(s) 1839, 3046, 3122
EcoT38I GRGCYC 4 cut(s) 1269, 1297, 1635, 2792
ErhI CCWWGG 3 cut(s) 1626, 2350, 3027
Esp3I CGTCTC 1 cut(s) 1516
FaqI GGGAC 2 cut(s) 773, 2864
FauI CCCGC 1 cut(s) 2876
FblI GTMKAC 1 cut(s) 1382
FokI GGATG 6 cut(s) 402, 563, 1448, 2771, 2881, 3262
FriOI GRGCYC 4 cut(s) 1269, 1297, 1635, 2792
FspBI CTAG 4 cut(s) 347, 354, 516, 1113
FspI TGCGCA 1 cut(s) 3090
GlaI GCGC 4 cut(s) 321, 631, 3090, 3401
GsaI CCCAGC 2 cut(s) 97, 3174
HaeIII GGCC 7 cut(s) 5, 128, 1006, 1198, 1633, 1929, 2126
HapII CCGG 4 cut(s) 55, 1003, 1810, 2486
HgaI GACGC 5 cut(s) 43, 232, 621, 2237, 3066
HhaI GCGC 4 cut(s) 322, 632, 3091, 3402
Hin1I GRCGYC 4 cut(s) 35, 224, 2248, 3077
Hin6I GCGC 4 cut(s) 320, 630, 3089, 3400
HinP1I GCGC 4 cut(s) 320, 630, 3089, 3400
HincII GTYRAC 1 cut(s) 834
HindII GTYRAC 1 cut(s) 834
HindIII AAGCTT 2 cut(s) 680, 3420
HpaII CCGG 4 cut(s) 55, 1003, 1810, 2486
HphI GGTGA 4 cut(s) 158, 771, 1157, 2836
Hpy166II GTNNAC 9 cut(s) 834, 1370, 1383, 1564, 2443, 2483, 3081, 3374, 3500
Hpy8I GTNNAC 9 cut(s) 834, 1370, 1383, 1564, 2443, 2483, 3081, 3374, 3500
Hpy99I CGWCG 5 cut(s) 34, 37, 155, 167, 170
HpyCH4III ACNGT 8 cut(s) 254, 787, 1387, 1679, 2087, 2548, 2734, 3050
HpyCH4IV ACGT 7 cut(s) 374, 489, 599, 1706, 2046, 3310, 3350
HpySE526I ACGT 7 cut(s) 374, 489, 599, 1706, 2046, 3310, 3350
Hsp92I GRCGYC 4 cut(s) 35, 224, 2248, 3077
HspAI GCGC 4 cut(s) 320, 630, 3089, 3400
KpnI GGTACC 1 cut(s) 2229
LguI GCTCTTC 1 cut(s) 2367
LmnI GCTCC 7 cut(s) 1264, 1723, 2390, 2609, 2870, 3440, 3481
Lsp1109I GCAGC 7 cut(s) 928, 1232, 1610, 1713, 1770, 1926, 3103
MaeI CTAG 4 cut(s) 347, 354, 516, 1113
MaeII ACGT 7 cut(s) 374, 489, 599, 1706, 2046, 3310, 3350
MbiI CCGCTC 2 cut(s) 1792, 2393
MfeI CAATTG 3 cut(s) 2679, 3084, 3333
MflI RGATCY 2 cut(s) 1105, 1827
MhlI GDGCHC 8 cut(s) 1240, 1269, 1297, 1635, 2445, 2792, 3376, 3502
MlsI TGGCCA 1 cut(s) 1929
MluNI TGGCCA 1 cut(s) 1929
MlyI GAGTC 6 cut(s) 32, 193, 336, 538, 1124, 3044
MmeI TCCRAC 3 cut(s) 721, 1265, 2238
Mox20I TGGCCA 1 cut(s) 1929
Mph1103I ATGCAT 3 cut(s) 1839, 3046, 3122
MroXI GAANNNNTTC 3 cut(s) 397, 1065, 2061
MscI TGGCCA 1 cut(s) 1929
MslI CAYNNNNRTG 2 cut(s) 602, 1279
Msp20I TGGCCA 1 cut(s) 1929
MspA1I CMGCKG 1 cut(s) 2822
MspCI CTTAAG 1 cut(s) 3487
MspI CCGG 4 cut(s) 55, 1003, 1810, 2486
MspR9I CCNGG 4 cut(s) 792, 1811, 1856, 1957
MunI CAATTG 3 cut(s) 2679, 3084, 3333
Mva1269I GAATGC 3 cut(s) 632, 1729, 3206
MvaI CCWGG 3 cut(s) 792, 1856, 1957
MvnI CGCG 2 cut(s) 131, 632
NciI CCSGG 1 cut(s) 1811
NlaIV GGNNCC 7 cut(s) 1632, 1633, 1853, 1903, 2227, 2611, 2866
NmuCI GTSAC 7 cut(s) 146, 331, 375, 1127, 1655, 2824, 3056
NsbI TGCGCA 1 cut(s) 3090
NsiI ATGCAT 3 cut(s) 1839, 3046, 3122
NspI RCATGY 3 cut(s) 2098, 2726, 2971
NspV TTCGAA 1 cut(s) 3009
PaeR7I CTCGAG 3 cut(s) 104, 324, 929
PagI TCATGA 3 cut(s) 769, 1480, 2164
PasI CCCWGGG 1 cut(s) 1956
PciI ACATGT 1 cut(s) 2967
PciSI GCTCTTC 1 cut(s) 2367
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 3 cut(s) 632, 1729, 3206
PdmI GAANNNNTTC 3 cut(s) 397, 1065, 2061
PfeI GAWTC 9 cut(s) 8, 526, 1040, 1475, 2285, 2489, 2710, 2843, 3002
PflMI CCANNNNNTGG 1 cut(s) 1432
PleI GAGTC 6 cut(s) 31, 192, 335, 538, 1124, 3043
PmaCI CACGTG 1 cut(s) 3311
PmlI CACGTG 1 cut(s) 3311
PpsI GAGTC 6 cut(s) 31, 192, 335, 538, 1124, 3043
Ppu21I YACGTR 1 cut(s) 3311
PscI ACATGT 1 cut(s) 2967
PshBI ATTAAT 1 cut(s) 291
Psp124BI GAGCTC 1 cut(s) 1269
Psp6I CCWGG 3 cut(s) 790, 1854, 1955
PspCI CACGTG 1 cut(s) 3311
PspEI GGTNACC 1 cut(s) 1031
PspFI CCCAGC 2 cut(s) 93, 3170
PspGI CCWGG 3 cut(s) 790, 1854, 1955
PspN4I GGNNCC 7 cut(s) 1632, 1633, 1853, 1903, 2227, 2611, 2866
PspOMI GGGCCC 1 cut(s) 1631
PspPI GGNCC 7 cut(s) 64, 521, 614, 1196, 1631, 1632, 2408
PspXI VCTCGAGB 2 cut(s) 104, 324
PstI CTGCAG 1 cut(s) 1222
PstNI CAGNNNCTG 2 cut(s) 791, 2963
PsuI RGATCY 2 cut(s) 1105, 1827
RsaI GTAC 8 cut(s) 274, 1001, 2227, 2484, 2640, 3199, 3339, 3353
RsaNI GTAC 8 cut(s) 273, 1000, 2226, 2483, 2639, 3198, 3338, 3352
RseI CAYNNNNRTG 2 cut(s) 602, 1279
SacI GAGCTC 1 cut(s) 1269
SapI GCTCTTC 1 cut(s) 2367
Sau96I GGNCC 7 cut(s) 64, 521, 614, 1196, 1631, 1632, 2408
SchI GAGTC 6 cut(s) 32, 193, 336, 538, 1124, 3044
ScrFI CCNGG 4 cut(s) 792, 1811, 1856, 1957
SduI GDGCHC 8 cut(s) 1240, 1269, 1297, 1635, 2445, 2792, 3376, 3502
SfcI CTRYAG 4 cut(s) 1218, 1383, 2319, 3129
Sfr274I CTCGAG 3 cut(s) 104, 324, 929
SfuI TTCGAA 1 cut(s) 3009
SinI GGWCC 4 cut(s) 64, 521, 614, 2408
SlaI CTCGAG 3 cut(s) 104, 324, 929
SmiMI CAYNNNNRTG 2 cut(s) 602, 1279
SmlI CTYRAG 5 cut(s) 104, 324, 929, 1787, 3487
SmoI CTYRAG 5 cut(s) 104, 324, 929, 1787, 3487
SpeI ACTAGT 1 cut(s) 515
SsiI CCGC 9 cut(s) 129, 159, 1792, 2393, 2726, 2770, 2822, 2869, 3479
SspI AATATT 1 cut(s) 2634
SspMI CTAG 4 cut(s) 347, 354, 516, 1113
SstI GAGCTC 1 cut(s) 1269
StyD4I CCNGG 4 cut(s) 790, 1809, 1854, 1955
StyI CCWWGG 3 cut(s) 1626, 2350, 3027
TaaI ACNGT 8 cut(s) 254, 787, 1387, 1679, 2087, 2548, 2734, 3050
TaiI ACGT 7 cut(s) 377, 492, 602, 1709, 2049, 3313, 3353
TaqII GACCGA 1 cut(s) 3318
TauI GCSGC 5 cut(s) 131, 162, 1795, 2396, 2772
TfiI GAWTC 9 cut(s) 8, 526, 1040, 1475, 2285, 2489, 2710, 2843, 3002
TscAI CASTG 6 cut(s) 340, 1276, 1356, 1392, 2553, 3507
TseFI GTSAC 7 cut(s) 146, 331, 375, 1127, 1655, 2824, 3056
TseI GCWGC 7 cut(s) 916, 1220, 1598, 1726, 1783, 1939, 3091
Tsp45I GTSAC 7 cut(s) 146, 331, 375, 1127, 1655, 2824, 3056
TspGWI ACGGA 3 cut(s) 394, 851, 1498
TspRI CASTG 6 cut(s) 340, 1276, 1356, 1392, 2553, 3507
Van91I CCANNNNNTGG 1 cut(s) 1432
Vha464I CTTAAG 1 cut(s) 3487
VneI GTGCAC 3 cut(s) 2441, 3372, 3498
VpaK11BI GGWCC 4 cut(s) 64, 521, 614, 2408
VspI ATTAAT 1 cut(s) 291
XapI RAATTY 6 cut(s) 670, 761, 924, 1048, 3265, 3358
XceI RCATGY 3 cut(s) 2098, 2726, 2971
XhoI CTCGAG 3 cut(s) 104, 324, 929
XmiI GTMKAC 1 cut(s) 1382
XmnI GAANNNNTTC 3 cut(s) 397, 1065, 2061
XspI CTAG 4 cut(s) 347, 354, 516, 1113
Zsp2I ATGCAT 3 cut(s) 1839, 3046, 3122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.