MD15G1365100.v1.1

Metallophosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013686
Physical Location & Seq
Forward (+)
44207311 .. 44218646
11336 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1365100.v1.1.491

Sequence Viewer

Length: 894 bp
ATGCTGCTGGTTTTGGGCGATATCTCGGCGCGGGGTCACAATTTGCCGAAAACCAAGTACGTTTCGGTGATCCACCAGTTCCACAGGGTACTGGGTCCGTTTCTGGGGCTGCCGCTCCACGTTGTTCTTGGAGACCGGGATATCGGAGAATGCGATGAGCTGAGTGCGGAGTCGGTGAGTTGGGTTTCGAGGAGGTTGCCGGGTCTGGACTCCGCCGGCTGCGGAGCTTTCGAAATCGGAAATGTGAGCTTTGTGTCGCTTAGTGCGGTGGCGTTGCTTTGTGGGAACAATGAGCTGAGGTTCAGTGTCGAGAAGGTGGTGGAGAGAGAAAGCATGGATTTTCAGATGGAGAGGGAGAGAGTGAATGAGGGAGGGGATGAAAACGACCGATTAGGAAAGAGGGTTTATGAGTTTGGGTGGAGAGAGAATGCTATGTCATCTGGGTCCGGTCCTGTGCTCTTGCTTCACTTCCCATTAAACCGGAATGTGAATGCCGCTAACAATGGTGGGAGTTCAAGCATATTGGATGGCAGGGGGGCTGTTGGTACAGGGCCATACAAGTTGTCGCACAAGCTACCGCTAAATGCTACTGAGTATATCTTTCAAGCTCTCAAACCAAGGATTGTTTTCAGCGCTCACACCCAAGAATTCTATGATCACTTCCATCCAGATGGAACTCGCGAGGTGACTGTTCCGGCCATGACGTGGAATGCAAGGGACGATCCTGGATTTGTTGTTGCCATTTTCCGGGGGAACAAAGGAGCAGTAAGCGTCAGCTATTGCTCACTTGCGAGGGAATCTCACGTTCTTGTTGCCTATGTATCTCTTCTGATTCTTTTGTTATCAACGATAATTTTCTCAACACCTGCTCCGAGATGTTTAAGACAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

298

Amino Acids

32.65

Weight (kDa)

6.8

Isoelectric Point (pI)

29.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013686)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g37850 FvH4_5g37850
malus_domestica MD15G1365100.v1.1
prunus_persica Prupe.1G513100_v2.0.a1
pyrus_communis pycom15g32740
rosa_chinensis RchiOBHm_Chr7g0239831
rosa_laevigata RLG00000000727
rosa_multiflora Rmu_sc0000540.1_g000066 Rmu_sc0007193.1_g000009
rosa_roxburghii Rroxscaffold_3G00220780
rosa_rugosa Rorug07G0329600 Rorug07G0329700 Rorug07G0329800
rosa_samantha Rh7AG483800 Rh7BG456800 Rh7CG501300 Rh7DG469500
rosa_wichuraiana Rw7G040580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 874
Acc36I ACCTGC 1 cut(s) 874
AccB7I CCANNNNNTGG 1 cut(s) 705
AccBSI CCGCTC 1 cut(s) 115
AccII CGCG 2 cut(s) 31, 681
AciI CCGC 8 cut(s) 31, 113, 167, 213, 222, 266, 495, 578
AclWI GGATC 2 cut(s) 64, 716
AcoI YGGCCR 1 cut(s) 696
AcsI RAATTY 1 cut(s) 647
AfaI GTAC 3 cut(s) 59, 90, 547
AfeI AGCGCT 1 cut(s) 634
AfiI CCNNNNNNNGG 3 cut(s) 104, 705, 747
AgsI TTSAA 2 cut(s) 516, 605
AjiI CACGTC 1 cut(s) 705
AjnI CCWGG 1 cut(s) 724
AluBI AGCT 7 cut(s) 160, 227, 249, 295, 574, 608, 777
AluI AGCT 7 cut(s) 160, 227, 249, 295, 574, 608, 777
Alw21I GWGCWC 1 cut(s) 459
Alw26I GTCTC 1 cut(s) 126
AlwI GGATC 2 cut(s) 64, 716
Aor51HI AGCGCT 1 cut(s) 634
AoxI GGCC 2 cut(s) 551, 696
ApeKI GCWGC 3 cut(s) 4, 109, 219
ApoI RAATTY 1 cut(s) 647
AspLEI GCGC 2 cut(s) 31, 635
AspS9I GGNCC 4 cut(s) 95, 444, 449, 551
AsuC2I CCSGG 3 cut(s) 137, 201, 749
AsuHPI GGTGA 3 cut(s) 79, 187, 697
AsuII TTCGAA 1 cut(s) 231
AvaII GGWCC 3 cut(s) 95, 444, 449
Bbv12I GWGCWC 1 cut(s) 459
BbvCI CCTCAGC 1 cut(s) 296
BbvI GCAGC 2 cut(s) 96, 206
BccI CCATC 4 cut(s) 340, 521, 665, 672
BcgI CGANNNNNNTGC 2 cut(s) 178, 212
BciT130I CCWGG 1 cut(s) 726
BclI TGATCA 1 cut(s) 655
BcnI CCSGG 3 cut(s) 137, 201, 749
BcoDI GTCTC 1 cut(s) 126
BfoI RGCGCY 1 cut(s) 636
BfuAI ACCTGC 1 cut(s) 874
BisI GCNGC 5 cut(s) 5, 110, 113, 220, 495
BlsI GCNGC 5 cut(s) 6, 111, 114, 221, 496
Bme1390I CCNGG 4 cut(s) 137, 201, 726, 749
Bme18I GGWCC 3 cut(s) 95, 444, 449
BmgBI CACGTC 1 cut(s) 705
BmgT120I GGNCC 4 cut(s) 95, 444, 449, 551
BmiI GGNNCC 2 cut(s) 96, 445
BmrFI CCNGG 4 cut(s) 137, 201, 726, 749
BmrI ACTGGG 1 cut(s) 101
BmuI ACTGGG 1 cut(s) 101
BplI GAGNNNNNCTC 2 cut(s) 784, 816
Bpu10I CCTNAGC 1 cut(s) 296
Bpu14I TTCGAA 1 cut(s) 231
BpuMI CCSGG 3 cut(s) 137, 201, 749
BsaI GGTCTC 1 cut(s) 126
BsaJI CCNNGG 2 cut(s) 617, 748
BsaWI WCCGGW 2 cut(s) 446, 480
BsaXI ACNNNNNCTCC 2 cut(s) 853, 883
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 705, 747
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 2 cut(s) 76, 96
BseBI CCWGG 1 cut(s) 726
BseDI CCNNGG 2 cut(s) 617, 748
BseGI GGATG 3 cut(s) 382, 532, 664
BseLI CCNNNNNNNGG 3 cut(s) 104, 705, 747
BseMII CTCAG 3 cut(s) 152, 287, 582
BseNI ACTGG 2 cut(s) 76, 96
BseRI GAGGAG 1 cut(s) 205
BseXI GCAGC 2 cut(s) 96, 206
Bsh1236I CGCG 2 cut(s) 31, 681
Bsh1285I CGRYCG 1 cut(s) 388
BshFI GGCC 2 cut(s) 553, 698
BsiEI CGRYCG 1 cut(s) 388
BsiHKAI GWGCWC 1 cut(s) 459
BsiSI CCGG 7 cut(s) 136, 200, 216, 447, 481, 695, 748
BslFI GGGAC 1 cut(s) 731
BslI CCNNNNNNNGG 3 cut(s) 104, 705, 747
BsmAI GTCTC 1 cut(s) 126
BsmFI GGGAC 1 cut(s) 731
BsmI GAATGC 4 cut(s) 155, 433, 496, 715
BsnI GGCC 2 cut(s) 553, 698
Bso31I GGTCTC 1 cut(s) 126
Bsp119I TTCGAA 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 459
Bsp143I GATC 3 cut(s) 69, 655, 721
Bsp68I TCGCGA 1 cut(s) 681
BspACI CCGC 8 cut(s) 31, 113, 167, 213, 222, 266, 495, 578
BspANI GGCC 2 cut(s) 553, 698
BspCNI CTCAG 3 cut(s) 153, 288, 583
BspFNI CGCG 2 cut(s) 31, 681
BspLI GGNNCC 2 cut(s) 96, 445
BspMI ACCTGC 1 cut(s) 874
BspPI GGATC 2 cut(s) 64, 716
BspT104I TTCGAA 1 cut(s) 231
BspTNI GGTCTC 1 cut(s) 126
BsrBI CCGCTC 1 cut(s) 115
BsrFI RCCGGY 1 cut(s) 215
BsrI ACTGG 2 cut(s) 76, 96
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 2 cut(s) 617, 748
BssMI GATC 3 cut(s) 69, 655, 721
BssT1I CCWWGG 1 cut(s) 617
Bst2UI CCWGG 1 cut(s) 726
Bst4CI ACNGT 1 cut(s) 691
Bst6I CTCTTC 1 cut(s) 831
BstBI TTCGAA 1 cut(s) 231
BstC8I GCNNGC 1 cut(s) 217
BstDEI CTNAG 4 cut(s) 161, 260, 296, 591
BstF5I GGATG 3 cut(s) 382, 532, 664
BstFNI CGCG 2 cut(s) 31, 681
BstH2I RGCGCY 1 cut(s) 636
BstHHI GCGC 2 cut(s) 31, 635
BstKTI GATC 3 cut(s) 72, 658, 724
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 3 cut(s) 69, 655, 721
BstMCI CGRYCG 1 cut(s) 388
BstNI CCWGG 1 cut(s) 726
BstSCI CCNGG 4 cut(s) 135, 199, 724, 747
BstUI CGCG 2 cut(s) 31, 681
BstV1I GCAGC 2 cut(s) 96, 206
BstXI CCANNNNNNTGG 1 cut(s) 671
BsuRI GGCC 2 cut(s) 553, 698
BtgZI GCGATG 1 cut(s) 168
BtrI CACGTC 1 cut(s) 705
BtsCI GGATG 3 cut(s) 382, 532, 664
BtsIMutI CAGTG 1 cut(s) 310
BtuMI TCGCGA 1 cut(s) 681
BveI ACCTGC 1 cut(s) 874
Cac8I GCNNGC 1 cut(s) 217
CfoI GCGC 2 cut(s) 31, 635
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 4 cut(s) 95, 444, 449, 551
CseI GACGC 1 cut(s) 760
Csp6I GTAC 3 cut(s) 58, 89, 546
CviAII CATG 2 cut(s) 334, 700
CviQI GTAC 3 cut(s) 58, 89, 546
DdeI CTNAG 4 cut(s) 161, 260, 296, 591
DpnI GATC 3 cut(s) 71, 657, 723
DpnII GATC 3 cut(s) 69, 655, 721
EaeI YGGCCR 1 cut(s) 696
Eam1104I CTCTTC 1 cut(s) 831
EarI CTCTTC 1 cut(s) 831
EciI GGCGGA 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 617
Eco31I GGTCTC 1 cut(s) 126
Eco32I GATATC 2 cut(s) 22, 142
Eco47I GGWCC 3 cut(s) 95, 444, 449
Eco47III AGCGCT 1 cut(s) 634
EcoRI GAATTC 1 cut(s) 647
EcoRII CCWGG 1 cut(s) 724
EcoRV GATATC 2 cut(s) 22, 142
EcoT14I CCWWGG 1 cut(s) 617
ErhI CCWWGG 1 cut(s) 617
FaeI CATG 2 cut(s) 337, 703
FaiI YATR 9 cut(s) 335, 408, 434, 521, 556, 597, 654, 701, 819
FaqI GGGAC 1 cut(s) 731
FatI CATG 2 cut(s) 333, 699
FauI CCCGC 1 cut(s) 24
FbaI TGATCA 1 cut(s) 655
Fnu4HI GCNGC 5 cut(s) 5, 110, 113, 220, 495
FokI GGATG 3 cut(s) 389, 539, 651
Fsp4HI GCNGC 5 cut(s) 5, 110, 113, 220, 495
GlaI GCGC 2 cut(s) 30, 634
GluI GCNGC 5 cut(s) 5, 110, 113, 220, 495
HaeII RGCGCY 1 cut(s) 636
HaeIII GGCC 2 cut(s) 553, 698
HapII CCGG 7 cut(s) 136, 200, 216, 447, 481, 695, 748
HgaI GACGC 1 cut(s) 760
HhaI GCGC 2 cut(s) 31, 635
Hin1II CATG 2 cut(s) 337, 703
Hin6I GCGC 2 cut(s) 29, 633
HinP1I GCGC 2 cut(s) 29, 633
HinfI GANTC 4 cut(s) 170, 209, 797, 832
HpaII CCGG 7 cut(s) 136, 200, 216, 447, 481, 695, 748
HphI GGTGA 3 cut(s) 79, 187, 697
Hpy188I TCNGA 5 cut(s) 146, 239, 345, 831, 873
Hpy188III TCNNGA 4 cut(s) 206, 310, 668, 680
HpyAV CCTTC 1 cut(s) 307
HpyCH4III ACNGT 1 cut(s) 691
HpyCH4IV ACGT 4 cut(s) 60, 120, 704, 804
HpyCH4V TGCA 1 cut(s) 713
HpyF3I CTNAG 4 cut(s) 161, 260, 296, 591
HpySE526I ACGT 4 cut(s) 60, 120, 704, 804
Hsp92II CATG 2 cut(s) 337, 703
HspAI GCGC 2 cut(s) 29, 633
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 655
Kzo9I GATC 3 cut(s) 69, 655, 721
LmnI GCTCC 4 cut(s) 120, 224, 761, 874
Lsp1109I GCAGC 2 cut(s) 96, 206
MaeII ACGT 4 cut(s) 60, 120, 704, 804
MaeIII GTNAC 2 cut(s) 35, 685
MalI GATC 3 cut(s) 71, 657, 723
MbiI CCGCTC 1 cut(s) 115
MboI GATC 3 cut(s) 69, 655, 721
MboII GAAGA 1 cut(s) 818
MhlI GDGCHC 1 cut(s) 459
MluCI AATT 3 cut(s) 40, 647, 852
MlyI GAGTC 2 cut(s) 179, 203
MnlI CCTC 9 cut(s) 183, 186, 291, 345, 361, 365, 393, 676, 786
MroNI GCCGGC 1 cut(s) 215
MseI TTAA 2 cut(s) 476, 881
MslI CAYNNNNRTG 1 cut(s) 669
MspI CCGG 7 cut(s) 136, 200, 216, 447, 481, 695, 748
MspR9I CCNGG 4 cut(s) 137, 201, 726, 749
Mva1269I GAATGC 4 cut(s) 155, 433, 496, 715
MvaI CCWGG 1 cut(s) 726
MvnI CGCG 2 cut(s) 31, 681
NaeI GCCGGC 1 cut(s) 217
NciI CCSGG 3 cut(s) 137, 201, 749
NdeII GATC 3 cut(s) 69, 655, 721
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 2 cut(s) 337, 703
NlaIV GGNNCC 2 cut(s) 96, 445
NmeAIII GCCGAG 1 cut(s) 5
NmuCI GTSAC 2 cut(s) 35, 685
NruI TCGCGA 1 cut(s) 681
NspV TTCGAA 1 cut(s) 231
PaqCI CACCTGC 1 cut(s) 874
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PctI GAATGC 4 cut(s) 155, 433, 496, 715
PdiI GCCGGC 1 cut(s) 217
PfeI GAWTC 2 cut(s) 797, 832
PflMI CCANNNNNTGG 1 cut(s) 705
PfoI TCCNGGA 1 cut(s) 724
PkrI GCNGC 5 cut(s) 6, 111, 114, 221, 496
PleI GAGTC 2 cut(s) 178, 203
PpsI GAGTC 2 cut(s) 178, 203
Psp6I CCWGG 1 cut(s) 724
PspGI CCWGG 1 cut(s) 724
PspN4I GGNNCC 2 cut(s) 96, 445
PspPI GGNCC 4 cut(s) 95, 444, 449, 551
RruI TCGCGA 1 cut(s) 681
RsaI GTAC 3 cut(s) 59, 90, 547
RsaNI GTAC 3 cut(s) 58, 89, 546
RseI CAYNNNNRTG 1 cut(s) 669
SaqAI TTAA 2 cut(s) 476, 881
SatI GCNGC 5 cut(s) 5, 110, 113, 220, 495
Sau3AI GATC 3 cut(s) 69, 655, 721
Sau96I GGNCC 4 cut(s) 95, 444, 449, 551
SchI GAGTC 2 cut(s) 179, 203
ScrFI CCNGG 4 cut(s) 137, 201, 726, 749
SduI GDGCHC 1 cut(s) 459
SfuI TTCGAA 1 cut(s) 231
SinI GGWCC 3 cut(s) 95, 444, 449
SmiMI CAYNNNNRTG 1 cut(s) 669
Sse9I AATT 3 cut(s) 40, 647, 852
SsiI CCGC 8 cut(s) 31, 113, 167, 213, 222, 266, 495, 578
StyD4I CCNGG 4 cut(s) 135, 199, 724, 747
StyI CCWWGG 1 cut(s) 617
TaaI ACNGT 1 cut(s) 691
TaiI ACGT 4 cut(s) 63, 123, 707, 807
TaqI TCGA 3 cut(s) 188, 231, 309
TaqII GACCGA 1 cut(s) 402
TasI AATT 3 cut(s) 40, 647, 852
TauI GCSGC 2 cut(s) 115, 497
TfiI GAWTC 2 cut(s) 797, 832
Tru1I TTAA 2 cut(s) 476, 881
Tru9I TTAA 2 cut(s) 476, 881
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 2 cut(s) 35, 685
TseI GCWGC 3 cut(s) 4, 109, 219
Tsp45I GTSAC 2 cut(s) 35, 685
TspDTI ATGAA 1 cut(s) 393
TspGWI ACGGA 1 cut(s) 87
TspRI CASTG 1 cut(s) 310
Van91I CCANNNNNTGG 1 cut(s) 705
VpaK11BI GGWCC 3 cut(s) 95, 444, 449
XapI RAATTY 1 cut(s) 647
XcmI CCANNNNNNNNNTGG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.