Rroxscaffold_3G00220780

Metallophosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013686
Physical Location & Seq
Forward (+)
3162618 .. 3167725
5108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00220780.1

Sequence Viewer

Length: 1281 bp
ATGGCGGTTGCCTTGAAGCCATTGTTGCCCATCATCATACTCTCCTCTCTGCTCCTCTACGAAGAGTGGGTCTCAACTCCCTCATGCAAACCCGAACCACCCGGACCCGAACCCGACCCGACCCAGGCCCATGCCCACCCGGACGACCTCAAGGTCATGATGGTCGCCAACCTCCTCCTCCTTGGCTCCGAATCCGGCTACTTCGACCTCCTCTTCCGCGACTACTACACCTCCAAATTCTTCACGAAATCGTTCCGGACTCTCAGGCCCGATATGCTCCTGGTCCTCGGCGACATCTCGGCGCGTGGCCACCACTTGCGGAGGAGCGAGTACGTCTCGGTGATCCGTCAGTTCCATCGGGTCCTGGGCCCGTTTCTGGACCTCCCGCTCCACGTGGTTCTCGGCGACCGGGACGTCGGAGTCTGCGACCGGCTGAGTGAGGAGTCGGTGGGCTGGATCGCGAGGAGGTTGCCGGGTTTGGACCCGGCCGGGTGCGGAGCCTTTGAGATTGGGAACGTCAGCTTCGTGTCGCTAAACGCCGTCGCTTTGCTGTGCGGGAATAGTGAGGTGAGGTTCAGTGTGGAGAAGGCGGTGGAGAGAGAGAGCGTGGATTTTCGAGTCGAAAATGAGGGCGGAAGTGAAGGTAGAGAGATGAAGGAGAGGGTTTATGAGTTCGAGTGGAGAGAGAATGCAATGGCGTCCGGGTCTGGACCTGTGGTTTTGCTTCACTTTCCATTGAGCCGAAATGTGAGTACTCCTGATTTTGGTGGGAGTCCTAATGTTTTGGACGGCAGGGAGGATGTTGGTAGAGGGCCGTACGATTTGTTACAGAAGCTACCGCCAAATGCTACTGAATACATATTTCAAGCTCTCAAGCCAAGGTCATTTTATGTCATTTTGAAAACTGAATTGCCCCCCGATGACTTGAGACTTGTATGCGTCTTTATTAGAATACTGTGTTATGTGATTGGCATTGCTCCTGTATATACAAATATGAGGATTGTTTTCAGTGCCCACACTCAGGAATTCTATGATCATTTCCATCCAGATGGAACCCGTGAGGTTACTGTTCCGGCCATGACATGGAATGCGAGGGATGATCCTGGATTTGTTATTGCCACTTTCCGAAGGAATGAAGGAGCAGTAAGCATCAGCCATTGCTCTCTTGCTAGGGAATCTCACATACTTGTAGCCTATGCATCTCTTCTGATTCTTTCGGTATCAATGCTGGCTTTCTCAAGCACACCTAGTTTAAGATCTTTCAAGACGATGATAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

426

Amino Acids

47.58

Weight (kDa)

5.78

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013686)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g37850 FvH4_5g37850
malus_domestica MD15G1365100.v1.1
prunus_persica Prupe.1G513100_v2.0.a1
pyrus_communis pycom15g32740
rosa_chinensis RchiOBHm_Chr7g0239831
rosa_laevigata RLG00000000727
rosa_multiflora Rmu_sc0000540.1_g000066 Rmu_sc0007193.1_g000009
rosa_roxburghii Rroxscaffold_3G00220780
rosa_rugosa Rorug07G0329600 Rorug07G0329700 Rorug07G0329800
rosa_samantha Rh7AG483800 Rh7BG456800 Rh7CG501300 Rh7DG469500
rosa_wichuraiana Rw7G040580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 152, 413, 419
AatII GACGTC 1 cut(s) 417
AccB7I CCANNNNNTGG 1 cut(s) 1083
AccBSI CCGCTC 1 cut(s) 388
AccII CGCG 3 cut(s) 219, 304, 461
AccIII TCCGGA 1 cut(s) 255
AciI CCGC 9 cut(s) 5, 217, 319, 386, 495, 555, 590, 633, 839
AclWI GGATC 3 cut(s) 337, 464, 1094
AcoI YGGCCR 3 cut(s) 307, 486, 1074
AcsI RAATTY 2 cut(s) 236, 1025
AcvI CACGTG 1 cut(s) 394
AcyI GRCGYC 2 cut(s) 414, 698
AfaI GTAC 3 cut(s) 332, 754, 818
AfiI CCNNNNNNNGG 5 cut(s) 124, 376, 764, 1021, 1083
AgsI TTSAA 4 cut(s) 16, 866, 901, 1264
AjnI CCWGG 4 cut(s) 123, 279, 363, 1102
AluBI AGCT 4 cut(s) 522, 835, 869, 1278
AluI AGCT 4 cut(s) 522, 835, 869, 1278
Alw26I GTCTC 3 cut(s) 76, 340, 922
AlwI GGATC 3 cut(s) 337, 464, 1094
Aor13HI TCCGGA 1 cut(s) 255
AoxI GGCC 7 cut(s) 126, 266, 307, 367, 486, 812, 1074
ApaI GGGCCC 1 cut(s) 371
ApoI RAATTY 2 cut(s) 236, 1025
ArsI GACNNNNNNTTYG 2 cut(s) 54, 86
Asp700I GAANNNNTTC 1 cut(s) 251
AspLEI GCGC 1 cut(s) 304
AsuC2I CCSGG 7 cut(s) 102, 140, 410, 474, 485, 490, 703
AsuHPI GGTGA 2 cut(s) 352, 580
AvaII GGWCC 6 cut(s) 104, 283, 361, 379, 481, 710
BaeGI GKGCMC 2 cut(s) 371, 1015
BalI TGGCCA 1 cut(s) 309
BanII GRGCYC 1 cut(s) 371
BbrPI CACGTG 1 cut(s) 394
BccI CCATC 5 cut(s) 38, 154, 363, 1043, 1050
BceAI ACGGC 3 cut(s) 524, 799, 805
BcgI CGANNNNNNTGC 2 cut(s) 451, 485
BciT130I CCWGG 4 cut(s) 125, 281, 365, 1104
BclI TGATCA 1 cut(s) 1033
BcnI CCSGG 7 cut(s) 102, 140, 410, 474, 485, 490, 703
BcoDI GTCTC 3 cut(s) 76, 340, 922
BfaI CTAG 2 cut(s) 1170, 1248
BglII AGATCT 1 cut(s) 1256
BmcAI AGTACT 1 cut(s) 754
Bme18I GGWCC 6 cut(s) 104, 283, 361, 379, 481, 710
BmiI GGNNCC 7 cut(s) 106, 187, 362, 369, 483, 499, 1054
BmsI GCATC 2 cut(s) 1158, 1208
BplI GAGNNNNNCTC 4 cut(s) 56, 88, 320, 352
BpuEI CTTGAG 4 cut(s) 134, 857, 946, 1222
BpuMI CCSGG 7 cut(s) 102, 140, 410, 474, 485, 490, 703
BsaAI YACGTR 1 cut(s) 394
BsaHI GRCGYC 2 cut(s) 414, 698
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 5 cut(s) 123, 181, 286, 364, 878
BsaWI WCCGGW 1 cut(s) 255
BsaXI ACNNNNNCTCC 2 cut(s) 215, 245
Bsc4I CCNNNNNNNGG 5 cut(s) 124, 376, 764, 1021, 1083
Bse118I RCCGGY 1 cut(s) 429
Bse3DI GCAATG 3 cut(s) 699, 972, 1156
BseAI TCCGGA 1 cut(s) 255
BseBI CCWGG 4 cut(s) 125, 281, 365, 1104
BseDI CCNNGG 5 cut(s) 123, 181, 286, 364, 878
BseGI GGATG 3 cut(s) 805, 1042, 1102
BseLI CCNNNNNNNGG 5 cut(s) 124, 376, 764, 1021, 1083
BseMI GCAATG 3 cut(s) 699, 972, 1156
BseMII CTCAG 3 cut(s) 277, 425, 1034
BseRI GAGGAG 8 cut(s) 34, 44, 164, 167, 200, 337, 455, 478
BseSI GKGCMC 2 cut(s) 371, 1015
BseX3I CGGCCG 1 cut(s) 486
Bsh1236I CGCG 3 cut(s) 219, 304, 461
Bsh1285I CGRYCG 3 cut(s) 409, 430, 489
BshFI GGCC 7 cut(s) 128, 268, 309, 369, 488, 814, 1076
BsiEI CGRYCG 3 cut(s) 409, 430, 489
BsiWI CGTACG 1 cut(s) 816
BslFI GGGAC 1 cut(s) 425
BslI CCNNNNNNNGG 5 cut(s) 124, 376, 764, 1021, 1083
BsmAI GTCTC 3 cut(s) 76, 340, 922
BsmBI CGTCTC 1 cut(s) 340
BsmFI GGGAC 1 cut(s) 425
BsmI GAATGC 2 cut(s) 694, 1093
BsnI GGCC 7 cut(s) 128, 268, 309, 369, 488, 814, 1076
Bso31I GGTCTC 1 cut(s) 76
Bsp120I GGGCCC 1 cut(s) 367
Bsp1286I GDGCHC 2 cut(s) 371, 1015
Bsp13I TCCGGA 1 cut(s) 255
Bsp143I GATC 5 cut(s) 342, 456, 1033, 1099, 1256
Bsp68I TCGCGA 1 cut(s) 461
BspACI CCGC 9 cut(s) 5, 217, 319, 386, 495, 555, 590, 633, 839
BspANI GGCC 7 cut(s) 128, 268, 309, 369, 488, 814, 1076
BspCNI CTCAG 3 cut(s) 276, 426, 1033
BspEI TCCGGA 1 cut(s) 255
BspFNI CGCG 3 cut(s) 219, 304, 461
BspHI TCATGA 1 cut(s) 156
BspLI GGNNCC 7 cut(s) 106, 187, 362, 369, 483, 499, 1054
BspPI GGATC 3 cut(s) 337, 464, 1094
BspTNI GGTCTC 1 cut(s) 76
BsrBI CCGCTC 1 cut(s) 388
BsrDI GCAATG 3 cut(s) 699, 972, 1156
BsrFI RCCGGY 1 cut(s) 429
BssAI RCCGGY 1 cut(s) 429
BssECI CCNNGG 5 cut(s) 123, 181, 286, 364, 878
BssMI GATC 5 cut(s) 342, 456, 1033, 1099, 1256
BssNI GRCGYC 2 cut(s) 414, 698
BssT1I CCWWGG 2 cut(s) 181, 878
Bst2UI CCWGG 4 cut(s) 125, 281, 365, 1104
Bst4CI ACNGT 2 cut(s) 957, 1069
Bst6I CTCTTC 3 cut(s) 57, 218, 1209
BstACI GRCGYC 2 cut(s) 414, 698
BstBAI YACGTR 1 cut(s) 394
BstC8I GCNNGC 1 cut(s) 1230
BstDEI CTNAG 3 cut(s) 263, 434, 1020
BstF5I GGATG 3 cut(s) 805, 1042, 1102
BstFNI CGCG 3 cut(s) 219, 304, 461
BstHHI GCGC 1 cut(s) 304
BstKTI GATC 5 cut(s) 345, 459, 1036, 1102, 1259
BstMAI GTCTC 3 cut(s) 76, 340, 922
BstMBI GATC 5 cut(s) 342, 456, 1033, 1099, 1256
BstMCI CGRYCG 3 cut(s) 409, 430, 489
BstMWI GCNNNNNNNGC 2 cut(s) 25, 274
BstNI CCWGG 4 cut(s) 125, 281, 365, 1104
BstSLI GKGCMC 2 cut(s) 371, 1015
BstUI CGCG 3 cut(s) 219, 304, 461
BstX2I RGATCY 1 cut(s) 1256
BstXI CCANNNNNNTGG 1 cut(s) 1049
BstYI RGATCY 1 cut(s) 1256
BstZI CGGCCG 1 cut(s) 486
BsuRI GGCC 7 cut(s) 128, 268, 309, 369, 488, 814, 1076
BtsCI GGATG 3 cut(s) 805, 1042, 1102
BtsIMutI CAGTG 2 cut(s) 583, 1015
BtuMI TCGCGA 1 cut(s) 461
Cac8I GCNNGC 1 cut(s) 1230
CciI TCATGA 1 cut(s) 156
CfoI GCGC 1 cut(s) 304
Cfr10I RCCGGY 1 cut(s) 429
CseI GACGC 2 cut(s) 687, 928
Csp6I GTAC 3 cut(s) 331, 753, 817
CviAII CATG 5 cut(s) 84, 131, 157, 1078, 1083
CviQI GTAC 3 cut(s) 331, 753, 817
DdeI CTNAG 3 cut(s) 263, 434, 1020
DpnI GATC 5 cut(s) 344, 458, 1035, 1101, 1258
DpnII GATC 5 cut(s) 342, 456, 1033, 1099, 1256
DrdI GACNNNNNNGTC 3 cut(s) 152, 413, 419
DseDI GACNNNNNNGTC 3 cut(s) 152, 413, 419
EaeI YGGCCR 3 cut(s) 307, 486, 1074
EagI CGGCCG 1 cut(s) 486
Eam1104I CTCTTC 3 cut(s) 57, 218, 1209
EarI CTCTTC 3 cut(s) 57, 218, 1209
EciI GGCGGA 1 cut(s) 648
EclXI CGGCCG 1 cut(s) 486
Eco130I CCWWGG 2 cut(s) 181, 878
Eco24I GRGCYC 1 cut(s) 371
Eco31I GGTCTC 1 cut(s) 76
Eco47I GGWCC 6 cut(s) 104, 283, 361, 379, 481, 710
Eco52I CGGCCG 1 cut(s) 486
Eco72I CACGTG 1 cut(s) 394
EcoO109I RGGNCCY 1 cut(s) 361
EcoRI GAATTC 1 cut(s) 1025
EcoRII CCWGG 4 cut(s) 123, 279, 363, 1102
EcoT14I CCWWGG 2 cut(s) 181, 878
EcoT22I ATGCAT 1 cut(s) 1201
EcoT38I GRGCYC 1 cut(s) 371
ErhI CCWWGG 2 cut(s) 181, 878
Esp3I CGTCTC 1 cut(s) 340
FaeI CATG 5 cut(s) 87, 134, 160, 1081, 1086
FaqI GGGAC 1 cut(s) 425
FatI CATG 5 cut(s) 83, 130, 156, 1077, 1082
FauI CCCGC 2 cut(s) 393, 548
FbaI TGATCA 1 cut(s) 1033
FokI GGATG 3 cut(s) 812, 1029, 1109
FriOI GRGCYC 1 cut(s) 371
FspBI CTAG 2 cut(s) 1170, 1248
GlaI GCGC 1 cut(s) 303
HaeIII GGCC 7 cut(s) 128, 268, 309, 369, 488, 814, 1076
HgaI GACGC 2 cut(s) 687, 928
HhaI GCGC 1 cut(s) 304
Hin1I GRCGYC 2 cut(s) 414, 698
Hin1II CATG 5 cut(s) 87, 134, 160, 1081, 1086
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HinfI GANTC 8 cut(s) 191, 259, 420, 443, 618, 772, 1175, 1210
HphI GGTGA 2 cut(s) 352, 580
Hpy188I TCNGA 4 cut(s) 190, 419, 1127, 1209
Hpy99I CGWCG 2 cut(s) 419, 545
HpyAV CCTTC 5 cut(s) 580, 635, 649, 1122, 1130
HpyCH4III ACNGT 2 cut(s) 957, 1069
HpyCH4IV ACGT 4 cut(s) 333, 393, 414, 516
HpyCH4V TGCA 3 cut(s) 87, 692, 1199
HpyF10VI GCNNNNNNNGC 2 cut(s) 25, 274
HpyF3I CTNAG 3 cut(s) 263, 434, 1020
HpySE526I ACGT 4 cut(s) 333, 393, 414, 516
Hsp92I GRCGYC 2 cut(s) 414, 698
Hsp92II CATG 5 cut(s) 87, 134, 160, 1081, 1086
HspAI GCGC 1 cut(s) 302
Kpn2I TCCGGA 1 cut(s) 255
Ksp22I TGATCA 1 cut(s) 1033
Kzo9I GATC 5 cut(s) 342, 456, 1033, 1099, 1256
LmnI GCTCC 8 cut(s) 57, 191, 282, 324, 393, 497, 982, 1139
LweI GCATC 2 cut(s) 1158, 1208
MaeI CTAG 2 cut(s) 1170, 1248
MaeII ACGT 4 cut(s) 333, 393, 414, 516
MaeIII GTNAC 2 cut(s) 825, 1063
MalI GATC 5 cut(s) 344, 458, 1035, 1101, 1258
MbiI CCGCTC 1 cut(s) 388
MboI GATC 5 cut(s) 342, 456, 1033, 1099, 1256
MboII GAAGA 4 cut(s) 74, 205, 232, 1196
MflI RGATCY 1 cut(s) 1256
MhlI GDGCHC 2 cut(s) 371, 1015
MlsI TGGCCA 1 cut(s) 309
MluCI AATT 3 cut(s) 236, 908, 1025
MluNI TGGCCA 1 cut(s) 309
MlyI GAGTC 5 cut(s) 253, 429, 452, 627, 781
MmeI TCCRAC 1 cut(s) 397
Mox20I TGGCCA 1 cut(s) 309
Mph1103I ATGCAT 1 cut(s) 1201
MroI TCCGGA 1 cut(s) 255
MroXI GAANNNNTTC 1 cut(s) 251
MscI TGGCCA 1 cut(s) 309
MseI TTAA 1 cut(s) 1253
MslI CAYNNNNRTG 1 cut(s) 1047
Msp20I TGGCCA 1 cut(s) 309
Mva1269I GAATGC 2 cut(s) 694, 1093
MvaI CCWGG 4 cut(s) 125, 281, 365, 1104
MvnI CGCG 3 cut(s) 219, 304, 461
MwoI GCNNNNNNNGC 2 cut(s) 25, 274
NciI CCSGG 7 cut(s) 102, 140, 410, 474, 485, 490, 703
NdeII GATC 5 cut(s) 342, 456, 1033, 1099, 1256
NlaIII CATG 5 cut(s) 87, 134, 160, 1081, 1086
NlaIV GGNNCC 7 cut(s) 106, 187, 362, 369, 483, 499, 1054
NmeAIII GCCGAG 3 cut(s) 267, 278, 381
NruI TCGCGA 1 cut(s) 461
NsiI ATGCAT 1 cut(s) 1201
PagI TCATGA 1 cut(s) 156
PcsI WCGNNNNNNNCGW 2 cut(s) 423, 522
PctI GAATGC 2 cut(s) 694, 1093
PdmI GAANNNNTTC 1 cut(s) 251
PfeI GAWTC 3 cut(s) 191, 1175, 1210
Pfl23II CGTACG 1 cut(s) 816
PflMI CCANNNNNTGG 1 cut(s) 1083
PfoI TCCNGGA 1 cut(s) 1102
PleI GAGTC 5 cut(s) 253, 428, 451, 626, 780
PmaCI CACGTG 1 cut(s) 394
PmlI CACGTG 1 cut(s) 394
PpsI GAGTC 5 cut(s) 253, 428, 451, 626, 780
Ppu21I YACGTR 1 cut(s) 394
PpuMI RGGWCCY 1 cut(s) 361
Psp5II RGGWCCY 1 cut(s) 361
Psp6I CCWGG 4 cut(s) 123, 279, 363, 1102
PspCI CACGTG 1 cut(s) 394
PspGI CCWGG 4 cut(s) 123, 279, 363, 1102
PspLI CGTACG 1 cut(s) 816
PspN4I GGNNCC 7 cut(s) 106, 187, 362, 369, 483, 499, 1054
PspOMI GGGCCC 1 cut(s) 367
PspPPI RGGWCCY 1 cut(s) 361
PsuI RGATCY 1 cut(s) 1256
RruI TCGCGA 1 cut(s) 461
RsaI GTAC 3 cut(s) 332, 754, 818
RsaNI GTAC 3 cut(s) 331, 753, 817
RseI CAYNNNNRTG 1 cut(s) 1047
SaqAI TTAA 1 cut(s) 1253
Sau3AI GATC 5 cut(s) 342, 456, 1033, 1099, 1256
ScaI AGTACT 1 cut(s) 754
SchI GAGTC 5 cut(s) 253, 429, 452, 627, 781
SduI GDGCHC 2 cut(s) 371, 1015
SfaNI GCATC 2 cut(s) 1158, 1208
SinI GGWCC 6 cut(s) 104, 283, 361, 379, 481, 710
SmiMI CAYNNNNRTG 1 cut(s) 1047
SmlI CTYRAG 4 cut(s) 149, 872, 925, 1237
SmoI CTYRAG 4 cut(s) 149, 872, 925, 1237
Sse9I AATT 3 cut(s) 236, 908, 1025
SsiI CCGC 9 cut(s) 5, 217, 319, 386, 495, 555, 590, 633, 839
SspMI CTAG 2 cut(s) 1170, 1248
StyI CCWWGG 2 cut(s) 181, 878
TaaI ACNGT 2 cut(s) 957, 1069
TaiI ACGT 4 cut(s) 336, 396, 417, 519
TaqI TCGA 4 cut(s) 204, 616, 621, 675
TasI AATT 3 cut(s) 236, 908, 1025
TatI WGTACW 1 cut(s) 752
TfiI GAWTC 3 cut(s) 191, 1175, 1210
Tru1I TTAA 1 cut(s) 1253
Tru9I TTAA 1 cut(s) 1253
TscAI CASTG 2 cut(s) 583, 1015
TspDTI ATGAA 2 cut(s) 668, 1149
TspGWI ACGGA 1 cut(s) 335
TspRI CASTG 2 cut(s) 583, 1015
Van91I CCANNNNNTGG 1 cut(s) 1083
VpaK11BI GGWCC 6 cut(s) 104, 283, 361, 379, 481, 710
XapI RAATTY 2 cut(s) 236, 1025
XmnI GAANNNNTTC 1 cut(s) 251
XspI CTAG 2 cut(s) 1170, 1248
ZraI GACGTC 1 cut(s) 415
ZrmI AGTACT 1 cut(s) 754
Zsp2I ATGCAT 1 cut(s) 1201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.