RLG00000000727

Metallophosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0013686
Physical Location & Seq
Forward (+)
4497998 .. 4501481
3484 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000727

Sequence Viewer

Length: 1197 bp
ATGGCGGTTGCCTTGAAGCAATTGTTGCCCATCATCGTACTCTCCTCTCTGCTCCTCTACGAAGAGTGGGTCTCAACTCCCTCATGCAAACCCGAACCACCCGGACCCGAACCCGACCCGGCCCAGGCGGCCCACCACCCGGACGACCTCAAGGTCATGATGGTCGCTAACCTCCTCCTCCTCGGCTCCGAATCCGGCTACTTCGACCTCCTCTTCCGCGACTACTACACCTCCAAATTCTTCACGAAATCGTTCCGGACTCTGAGGCCCGATATGCTCCTGGTCCTCGGCGACATCTCGGCGCGTGGCCACCACTTGCGGAGGAGCGAGTACGTCTCGGTGATCCGGCAGTTCCATCGGGTCCTGGGCCCGTTTCTGGACCTCCCGCTCCACGTGGTTCTCGGCGACCGGGATGTCGGAGTCTGCGACGGGCTGAGTGAGGAGTCGGTGAGCTGGATCGCGAGGAGGTTGCCGGGTTTGGACCCGGCCGGGTGCGGAGCCTTTGAGATTGGGAACGTCAGCTTCGTGTCGCTAAACGCCGTCGCTTTGCTGTGTAGGAATAGTGAGGTGAGGTTTAGTGTGGAGAAGGCGGTGGAGAGAGAGAGCGTGGATTTTCGAGTCGAAAATGAGGGGGGAAGTGGAGATAGAGAGAGGAAGGAGAGGGTTTATGAGTTTGGGTGGAGAGAGAATGCAATGTCCTCCGGGTCTGGACCTGTGGTTTTGCTTCACTTTCCGTTGAGCCGAAATGTGAGTACTACTGATTTCGAAGGGAGTCCTGATGTTTTCGACGGCAGGGAGGATGTTGGTAGAGGGCCATACGATTTGTTACAGAAGCTACCGCCAAATGCTACTGAATACATATTTCAAGCTCTCAAGCCAAGGATTGTTTTCAGTGCCCACACTCAGGAATTCTATGATCATTTCCATCCAGATGGAACCCGTGAGGTTACTGTTCCAGCCATGACATGGAATGCGAGGGATGATCCTGGATTTGTTATTGCCACTTTCCGAAGGAATGAAGGAGCAGTAAGCATCAGCCATTGCTCTCTTGCTAGGGAATCTCACATGCTTGTAGCCTATGCATCTCTTCTGATTCTTTCGCTCCATTTGGTTGTTTTGATATTCATTTTGTTGATGGTGGGGATGGAACTCGGAAGCGTTTTAGAAGCTGCAGCTGTAGGGTTGGCTCTTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

399

Amino Acids

44.18

Weight (kDa)

5.34

Isoelectric Point (pI)

36.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013686)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g37850 FvH4_5g37850
malus_domestica MD15G1365100.v1.1
prunus_persica Prupe.1G513100_v2.0.a1
pyrus_communis pycom15g32740
rosa_chinensis RchiOBHm_Chr7g0239831
rosa_laevigata RLG00000000727
rosa_multiflora Rmu_sc0000540.1_g000066 Rmu_sc0007193.1_g000009
rosa_roxburghii Rroxscaffold_3G00220780
rosa_rugosa Rorug07G0329600 Rorug07G0329700 Rorug07G0329800
rosa_samantha Rh7AG483800 Rh7BG456800 Rh7CG501300 Rh7DG469500
rosa_wichuraiana Rw7G040580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 152, 413
AccB7I CCANNNNNTGG 1 cut(s) 966
AccBSI CCGCTC 1 cut(s) 388
AccII CGCG 3 cut(s) 219, 304, 461
AccIII TCCGGA 1 cut(s) 255
AciI CCGC 8 cut(s) 5, 128, 217, 319, 386, 495, 590, 839
AclWI GGATC 3 cut(s) 337, 464, 977
AcoI YGGCCR 2 cut(s) 307, 486
AcsI RAATTY 2 cut(s) 236, 908
AcvI CACGTG 1 cut(s) 394
AfaI GTAC 3 cut(s) 39, 332, 754
AfiI CCNNNNNNNGG 5 cut(s) 124, 139, 376, 904, 966
AgsI TTSAA 2 cut(s) 16, 866
AjnI CCWGG 4 cut(s) 123, 279, 363, 985
AluBI AGCT 6 cut(s) 453, 522, 835, 869, 1169, 1175
AluI AGCT 6 cut(s) 453, 522, 835, 869, 1169, 1175
Alw26I GTCTC 2 cut(s) 76, 340
AlwI GGATC 3 cut(s) 337, 464, 977
Aor13HI TCCGGA 1 cut(s) 255
AoxI GGCC 7 cut(s) 120, 129, 266, 307, 367, 486, 812
ApaI GGGCCC 1 cut(s) 371
ApeKI GCWGC 2 cut(s) 1169, 1172
ApoI RAATTY 2 cut(s) 236, 908
ArsI GACNNNNNNTTYG 2 cut(s) 54, 86
Asp700I GAANNNNTTC 1 cut(s) 251
AspLEI GCGC 1 cut(s) 304
AsuC2I CCSGG 8 cut(s) 102, 119, 140, 410, 474, 485, 490, 703
AsuHPI GGTGA 3 cut(s) 352, 460, 580
AsuII TTCGAA 1 cut(s) 765
AvaII GGWCC 6 cut(s) 104, 283, 361, 379, 481, 710
BaeGI GKGCMC 2 cut(s) 371, 898
BalI TGGCCA 1 cut(s) 309
BanII GRGCYC 1 cut(s) 371
BbrPI CACGTG 1 cut(s) 394
BbvI GCAGC 2 cut(s) 1156, 1184
BccI CCATC 7 cut(s) 38, 154, 363, 926, 933, 1129, 1138
BceAI ACGGC 2 cut(s) 524, 805
BcgI CGANNNNNNTGC 4 cut(s) 338, 372, 451, 485
BciT130I CCWGG 4 cut(s) 125, 281, 365, 987
BclI TGATCA 1 cut(s) 916
BcnI CCSGG 8 cut(s) 102, 119, 140, 410, 474, 485, 490, 703
BcoDI GTCTC 2 cut(s) 76, 340
BfaI CTAG 1 cut(s) 1053
BfmI CTRYAG 2 cut(s) 1170, 1176
BglI GCCNNNNNGGC 1 cut(s) 128
BisI GCNGC 3 cut(s) 129, 1170, 1173
BlsI GCNGC 3 cut(s) 130, 1171, 1174
BmcAI AGTACT 1 cut(s) 754
Bme18I GGWCC 6 cut(s) 104, 283, 361, 379, 481, 710
BmiI GGNNCC 7 cut(s) 106, 187, 362, 369, 483, 499, 937
BmsI GCATC 2 cut(s) 1041, 1091
BplI GAGNNNNNCTC 4 cut(s) 56, 88, 320, 352
Bpu14I TTCGAA 1 cut(s) 765
BpuEI CTTGAG 2 cut(s) 134, 857
BpuMI CCSGG 8 cut(s) 102, 119, 140, 410, 474, 485, 490, 703
BsaAI YACGTR 1 cut(s) 394
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 5 cut(s) 123, 181, 286, 364, 878
BsaWI WCCGGW 1 cut(s) 255
BsaXI ACNNNNNCTCC 2 cut(s) 215, 245
Bsc4I CCNNNNNNNGG 5 cut(s) 124, 139, 376, 904, 966
Bse3DI GCAATG 2 cut(s) 699, 1039
BseAI TCCGGA 1 cut(s) 255
BseBI CCWGG 4 cut(s) 125, 281, 365, 987
BseDI CCNNGG 5 cut(s) 123, 181, 286, 364, 878
BseGI GGATG 5 cut(s) 418, 805, 925, 985, 1149
BseLI CCNNNNNNNGG 5 cut(s) 124, 139, 376, 904, 966
BseMI GCAATG 2 cut(s) 699, 1039
BseMII CTCAG 3 cut(s) 254, 425, 917
BseRI GAGGAG 9 cut(s) 34, 44, 164, 167, 170, 200, 337, 455, 478
BseSI GKGCMC 2 cut(s) 371, 898
BseX3I CGGCCG 1 cut(s) 486
BseXI GCAGC 2 cut(s) 1156, 1184
Bsh1236I CGCG 3 cut(s) 219, 304, 461
Bsh1285I CGRYCG 2 cut(s) 409, 489
BshFI GGCC 7 cut(s) 122, 131, 268, 309, 369, 488, 814
BsiEI CGRYCG 2 cut(s) 409, 489
BslI CCNNNNNNNGG 5 cut(s) 124, 139, 376, 904, 966
BsmAI GTCTC 2 cut(s) 76, 340
BsmBI CGTCTC 1 cut(s) 340
BsmI GAATGC 2 cut(s) 694, 976
BsnI GGCC 7 cut(s) 122, 131, 268, 309, 369, 488, 814
Bso31I GGTCTC 1 cut(s) 76
Bsp119I TTCGAA 1 cut(s) 765
Bsp120I GGGCCC 1 cut(s) 367
Bsp1286I GDGCHC 2 cut(s) 371, 898
Bsp13I TCCGGA 1 cut(s) 255
Bsp143I GATC 4 cut(s) 342, 456, 916, 982
Bsp68I TCGCGA 1 cut(s) 461
BspACI CCGC 8 cut(s) 5, 128, 217, 319, 386, 495, 590, 839
BspANI GGCC 7 cut(s) 122, 131, 268, 309, 369, 488, 814
BspCNI CTCAG 3 cut(s) 255, 426, 916
BspEI TCCGGA 1 cut(s) 255
BspFNI CGCG 3 cut(s) 219, 304, 461
BspHI TCATGA 1 cut(s) 156
BspLI GGNNCC 7 cut(s) 106, 187, 362, 369, 483, 499, 937
BspMAI CTGCAG 1 cut(s) 1174
BspPI GGATC 3 cut(s) 337, 464, 977
BspT104I TTCGAA 1 cut(s) 765
BspTNI GGTCTC 1 cut(s) 76
BsrBI CCGCTC 1 cut(s) 388
BsrDI GCAATG 2 cut(s) 699, 1039
BssECI CCNNGG 5 cut(s) 123, 181, 286, 364, 878
BssMI GATC 4 cut(s) 342, 456, 916, 982
BssT1I CCWWGG 1 cut(s) 878
Bst2UI CCWGG 4 cut(s) 125, 281, 365, 987
Bst4CI ACNGT 1 cut(s) 952
Bst6I CTCTTC 3 cut(s) 57, 218, 1092
BstAPI GCANNNNNTGC 1 cut(s) 25
BstBAI YACGTR 1 cut(s) 394
BstBI TTCGAA 1 cut(s) 765
BstDEI CTNAG 3 cut(s) 263, 434, 903
BstF5I GGATG 5 cut(s) 418, 805, 925, 985, 1149
BstFNI CGCG 3 cut(s) 219, 304, 461
BstHHI GCGC 1 cut(s) 304
BstKTI GATC 4 cut(s) 345, 459, 919, 985
BstMAI GTCTC 2 cut(s) 76, 340
BstMBI GATC 4 cut(s) 342, 456, 916, 982
BstMCI CGRYCG 2 cut(s) 409, 489
BstMWI GCNNNNNNNGC 3 cut(s) 25, 128, 274
BstNI CCWGG 4 cut(s) 125, 281, 365, 987
BstNSI RCATGY 1 cut(s) 1069
BstSFI CTRYAG 2 cut(s) 1170, 1176
BstSLI GKGCMC 2 cut(s) 371, 898
BstUI CGCG 3 cut(s) 219, 304, 461
BstV1I GCAGC 2 cut(s) 1156, 1184
BstXI CCANNNNNNTGG 1 cut(s) 932
BstZI CGGCCG 1 cut(s) 486
BsuRI GGCC 7 cut(s) 122, 131, 268, 309, 369, 488, 814
BtsCI GGATG 5 cut(s) 418, 805, 925, 985, 1149
BtsIMutI CAGTG 1 cut(s) 898
BtuMI TCGCGA 1 cut(s) 461
CciI TCATGA 1 cut(s) 156
CfoI GCGC 1 cut(s) 304
Csp6I GTAC 3 cut(s) 38, 331, 753
CviAII CATG 5 cut(s) 84, 157, 961, 966, 1066
CviQI GTAC 3 cut(s) 38, 331, 753
DdeI CTNAG 3 cut(s) 263, 434, 903
DpnI GATC 4 cut(s) 344, 458, 918, 984
DpnII GATC 4 cut(s) 342, 456, 916, 982
DrdI GACNNNNNNGTC 2 cut(s) 152, 413
DseDI GACNNNNNNGTC 2 cut(s) 152, 413
EaeI YGGCCR 2 cut(s) 307, 486
EagI CGGCCG 1 cut(s) 486
Eam1104I CTCTTC 3 cut(s) 57, 218, 1092
EarI CTCTTC 3 cut(s) 57, 218, 1092
EclXI CGGCCG 1 cut(s) 486
Eco130I CCWWGG 1 cut(s) 878
Eco24I GRGCYC 1 cut(s) 371
Eco31I GGTCTC 1 cut(s) 76
Eco47I GGWCC 6 cut(s) 104, 283, 361, 379, 481, 710
Eco52I CGGCCG 1 cut(s) 486
Eco72I CACGTG 1 cut(s) 394
EcoO109I RGGNCCY 1 cut(s) 361
EcoRI GAATTC 1 cut(s) 908
EcoRII CCWGG 4 cut(s) 123, 279, 363, 985
EcoT14I CCWWGG 1 cut(s) 878
EcoT22I ATGCAT 1 cut(s) 1084
EcoT38I GRGCYC 1 cut(s) 371
ErhI CCWWGG 1 cut(s) 878
Esp3I CGTCTC 1 cut(s) 340
FaeI CATG 5 cut(s) 87, 160, 964, 969, 1069
FatI CATG 5 cut(s) 83, 156, 960, 965, 1065
FauI CCCGC 1 cut(s) 393
FbaI TGATCA 1 cut(s) 916
Fnu4HI GCNGC 3 cut(s) 129, 1170, 1173
FokI GGATG 5 cut(s) 425, 812, 912, 992, 1156
FriOI GRGCYC 1 cut(s) 371
Fsp4HI GCNGC 3 cut(s) 129, 1170, 1173
FspBI CTAG 1 cut(s) 1053
GlaI GCGC 1 cut(s) 303
GluI GCNGC 3 cut(s) 129, 1170, 1173
HaeIII GGCC 7 cut(s) 122, 131, 268, 309, 369, 488, 814
HhaI GCGC 1 cut(s) 304
Hin1II CATG 5 cut(s) 87, 160, 964, 969, 1069
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HinfI GANTC 8 cut(s) 191, 259, 420, 443, 618, 772, 1058, 1093
HphI GGTGA 3 cut(s) 352, 460, 580
Hpy188I TCNGA 6 cut(s) 190, 264, 419, 1010, 1092, 1154
Hpy99I CGWCG 3 cut(s) 431, 545, 791
HpyAV CCTTC 5 cut(s) 580, 649, 761, 1005, 1013
HpyCH4III ACNGT 1 cut(s) 952
HpyCH4IV ACGT 3 cut(s) 333, 393, 516
HpyCH4V TGCA 4 cut(s) 87, 692, 1082, 1172
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 128, 274
HpyF3I CTNAG 3 cut(s) 263, 434, 903
HpySE526I ACGT 3 cut(s) 333, 393, 516
Hsp92II CATG 5 cut(s) 87, 160, 964, 969, 1069
HspAI GCGC 1 cut(s) 302
Kpn2I TCCGGA 1 cut(s) 255
Ksp22I TGATCA 1 cut(s) 916
Kzo9I GATC 4 cut(s) 342, 456, 916, 982
LmnI GCTCC 8 cut(s) 57, 191, 282, 324, 393, 497, 1022, 1107
Lsp1109I GCAGC 2 cut(s) 1156, 1184
LweI GCATC 2 cut(s) 1041, 1091
MaeI CTAG 1 cut(s) 1053
MaeII ACGT 3 cut(s) 333, 393, 516
MaeIII GTNAC 2 cut(s) 825, 946
MalI GATC 4 cut(s) 344, 458, 918, 984
MbiI CCGCTC 1 cut(s) 388
MboI GATC 4 cut(s) 342, 456, 916, 982
MboII GAAGA 4 cut(s) 74, 205, 232, 1079
MfeI CAATTG 1 cut(s) 20
MhlI GDGCHC 2 cut(s) 371, 898
MlsI TGGCCA 1 cut(s) 309
MluCI AATT 3 cut(s) 20, 236, 908
MluNI TGGCCA 1 cut(s) 309
MlyI GAGTC 5 cut(s) 253, 429, 452, 627, 781
MmeI TCCRAC 1 cut(s) 397
Mox20I TGGCCA 1 cut(s) 309
Mph1103I ATGCAT 1 cut(s) 1084
MroI TCCGGA 1 cut(s) 255
MroXI GAANNNNTTC 1 cut(s) 251
MscI TGGCCA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 930
Msp20I TGGCCA 1 cut(s) 309
MspA1I CMGCKG 1 cut(s) 1175
MunI CAATTG 1 cut(s) 20
Mva1269I GAATGC 2 cut(s) 694, 976
MvaI CCWGG 4 cut(s) 125, 281, 365, 987
MvnI CGCG 3 cut(s) 219, 304, 461
MwoI GCNNNNNNNGC 3 cut(s) 25, 128, 274
NciI CCSGG 8 cut(s) 102, 119, 140, 410, 474, 485, 490, 703
NdeII GATC 4 cut(s) 342, 456, 916, 982
NlaIII CATG 5 cut(s) 87, 160, 964, 969, 1069
NlaIV GGNNCC 7 cut(s) 106, 187, 362, 369, 483, 499, 937
NmeAIII GCCGAG 4 cut(s) 162, 267, 278, 381
NruI TCGCGA 1 cut(s) 461
NsiI ATGCAT 1 cut(s) 1084
NspI RCATGY 1 cut(s) 1069
NspV TTCGAA 1 cut(s) 765
PagI TCATGA 1 cut(s) 156
PcsI WCGNNNNNNNCGW 2 cut(s) 423, 522
PctI GAATGC 2 cut(s) 694, 976
PdmI GAANNNNTTC 1 cut(s) 251
PfeI GAWTC 3 cut(s) 191, 1058, 1093
PflMI CCANNNNNTGG 1 cut(s) 966
PfoI TCCNGGA 1 cut(s) 985
PkrI GCNGC 3 cut(s) 130, 1171, 1174
PleI GAGTC 5 cut(s) 253, 428, 451, 626, 780
PmaCI CACGTG 1 cut(s) 394
PmlI CACGTG 1 cut(s) 394
PpsI GAGTC 5 cut(s) 253, 428, 451, 626, 780
Ppu21I YACGTR 1 cut(s) 394
PpuMI RGGWCCY 1 cut(s) 361
Psp5II RGGWCCY 1 cut(s) 361
Psp6I CCWGG 4 cut(s) 123, 279, 363, 985
PspCI CACGTG 1 cut(s) 394
PspGI CCWGG 4 cut(s) 123, 279, 363, 985
PspN4I GGNNCC 7 cut(s) 106, 187, 362, 369, 483, 499, 937
PspOMI GGGCCC 1 cut(s) 367
PspPPI RGGWCCY 1 cut(s) 361
PstI CTGCAG 1 cut(s) 1174
PvuII CAGCTG 1 cut(s) 1175
RruI TCGCGA 1 cut(s) 461
RsaI GTAC 3 cut(s) 39, 332, 754
RsaNI GTAC 3 cut(s) 38, 331, 753
RseI CAYNNNNRTG 1 cut(s) 930
SatI GCNGC 3 cut(s) 129, 1170, 1173
Sau3AI GATC 4 cut(s) 342, 456, 916, 982
ScaI AGTACT 1 cut(s) 754
SchI GAGTC 5 cut(s) 253, 429, 452, 627, 781
SduI GDGCHC 2 cut(s) 371, 898
SfaNI GCATC 2 cut(s) 1041, 1091
SfcI CTRYAG 2 cut(s) 1170, 1176
SfiI GGCCNNNNNGGCC 1 cut(s) 128
SfuI TTCGAA 1 cut(s) 765
SinI GGWCC 6 cut(s) 104, 283, 361, 379, 481, 710
SmiMI CAYNNNNRTG 1 cut(s) 930
SmlI CTYRAG 2 cut(s) 149, 872
SmoI CTYRAG 2 cut(s) 149, 872
Sse9I AATT 3 cut(s) 20, 236, 908
SsiI CCGC 8 cut(s) 5, 128, 217, 319, 386, 495, 590, 839
SspMI CTAG 1 cut(s) 1053
StyI CCWWGG 1 cut(s) 878
TaaI ACNGT 1 cut(s) 952
TaiI ACGT 3 cut(s) 336, 396, 519
TaqI TCGA 5 cut(s) 204, 616, 621, 765, 786
TasI AATT 3 cut(s) 20, 236, 908
TatI WGTACW 1 cut(s) 752
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 3 cut(s) 191, 1058, 1093
TscAI CASTG 1 cut(s) 898
TseI GCWGC 2 cut(s) 1169, 1172
TspDTI ATGAA 2 cut(s) 1032, 1114
TspGWI ACGGA 1 cut(s) 723
TspRI CASTG 1 cut(s) 898
Van91I CCANNNNNTGG 1 cut(s) 966
VpaK11BI GGWCC 6 cut(s) 104, 283, 361, 379, 481, 710
XapI RAATTY 2 cut(s) 236, 908
XceI RCATGY 1 cut(s) 1069
XcmI CCANNNNNNNNNTGG 1 cut(s) 963
XmnI GAANNNNTTC 1 cut(s) 251
XspI CTAG 1 cut(s) 1053
ZrmI AGTACT 1 cut(s) 754
Zsp2I ATGCAT 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.