MD15G1382900.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005332
Physical Location & Seq
Reverse (-)
47169267 .. 47172742
3476 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1382900.v1.1.491

Sequence Viewer

Length: 765 bp
ATGGAGGTCGAGGTCGTCTCCGGCTCGAAACCCTCGGCCGACGCTATATTGAGGAGAGTGGATTCGCAGAAGGATCAGCTCCGCGTGAAGAGGAAGACTCTGCAGGCGGTGCTCGAGCAGTGCCAGAGAGCGCTCGAGTCGCTCGGTACAACTGGCGGTGCTGACGAGGAGGATAATGGAGATGATGGCGAAGAAGACGATGACGATAACAGTTCTCGTGGTTCGGCCGTTGACGACGAGCGCCAACAGGGCCGGGGATCGACTAGTTCGCGGGCTGATTGCGAGGCCGACGAGTTATGCGACCTTCTTAAATCGAGAGTTGAATGCCCCGACTTCCTCCAAAAGCTAGAAAGTGCACAAGCATTAGTTCCACAAAATATACCTGAAGAAGGTAATTCTTGGGATCTGGTTAGTGAGAATGATCTATGGGAAGATGAGGATAGCAACCTTGGTGAAGAAGATTATGTTCTTGTTAGGCAAGAAGATATAGTAGAGGGTATTGCGTGCTTTATGGCTGCATACCTGTTGTCCCTTAAACAGACTAAGGAATTGACCCCTAATCAACTTCAGGAAGCCCTTAGCAAAACATTCTCAGTGAAAACGAAGAAGGGAAAGCTTCGGAAGGCATGGGATGGAAGCAAAGTTATCTATAATGTAGCATCGTGGGGGGCAACTGCCATTGGGATATACCAAAACCCTGTGATCATAAGGGTTGCCACGAAAGCCTTCTGGACATCTTGTGAAGTTATATCAAAGTTTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

255

Amino Acids

28.13

Weight (kDa)

4.47

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 84, 271
AciI CCGC 4 cut(s) 82, 107, 156, 271
AclWI GGATC 3 cut(s) 81, 265, 411
AcoI YGGCCR 2 cut(s) 36, 225
AcuI CTGAAG 2 cut(s) 405, 551
AfaI GTAC 1 cut(s) 148
AfeI AGCGCT 1 cut(s) 132
AfiI CCNNNNNNNGG 1 cut(s) 389
AgsI TTSAA 1 cut(s) 323
AhlI ACTAGT 1 cut(s) 263
AluBI AGCT 3 cut(s) 79, 346, 616
AluI AGCT 3 cut(s) 79, 346, 616
Alw21I GWGCWC 2 cut(s) 114, 358
Alw26I GTCTC 1 cut(s) 22
Alw44I GTGCAC 1 cut(s) 354
AlwI GGATC 3 cut(s) 81, 265, 411
Ama87I CYCGRG 2 cut(s) 113, 134
Aor51HI AGCGCT 1 cut(s) 132
AoxI GGCC 4 cut(s) 36, 225, 250, 285
ApaLI GTGCAC 1 cut(s) 354
ApeKI GCWGC 1 cut(s) 515
Asp700I GAANNNNTTC 1 cut(s) 725
AspLEI GCGC 2 cut(s) 133, 243
AspS9I GGNCC 1 cut(s) 250
AsuC2I CCSGG 1 cut(s) 254
AsuHPI GGTGA 1 cut(s) 464
AvaI CYCGRG 2 cut(s) 113, 134
BaeGI GKGCMC 1 cut(s) 358
BauI CACGAG 1 cut(s) 216
BbsI GAAGAC 2 cut(s) 101, 201
Bbv12I GWGCWC 2 cut(s) 114, 358
BbvI GCAGC 1 cut(s) 502
BccI CCATC 2 cut(s) 179, 626
BceAI ACGGC 1 cut(s) 212
BclI TGATCA 1 cut(s) 702
BcnI CCSGG 1 cut(s) 254
BcoDI GTCTC 1 cut(s) 22
BcuI ACTAGT 1 cut(s) 263
BfaI CTAG 2 cut(s) 264, 347
BfmI CTRYAG 1 cut(s) 101
BfoI RGCGCY 2 cut(s) 134, 244
BglI GCCNNNNNGGC 1 cut(s) 249
BisI GCNGC 1 cut(s) 516
BlsI GCNGC 1 cut(s) 517
Bme1390I CCNGG 1 cut(s) 254
BmeT110I CYCGRG 2 cut(s) 113, 134
BmgT120I GGNCC 1 cut(s) 250
BmrFI CCNGG 1 cut(s) 254
BmsI GCATC 1 cut(s) 668
BpiI GAAGAC 2 cut(s) 101, 201
BplI GAGNNNNNCTC 3 cut(s) 34, 82, 114
Bpu10I CCTNAGC 1 cut(s) 578
BpuMI CCSGG 1 cut(s) 254
BsaJI CCNNGG 3 cut(s) 33, 253, 448
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse1I ACTGG 1 cut(s) 157
BseDI CCNNGG 3 cut(s) 33, 253, 448
BseGI GGATG 1 cut(s) 637
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMII CTCAG 1 cut(s) 606
BseNI ACTGG 1 cut(s) 157
BseRI GAGGAG 2 cut(s) 67, 182
BseSI GKGCMC 1 cut(s) 358
BseX3I CGGCCG 2 cut(s) 36, 225
BseXI GCAGC 1 cut(s) 502
Bsh1236I CGCG 2 cut(s) 84, 271
Bsh1285I CGRYCG 2 cut(s) 39, 228
BshFI GGCC 4 cut(s) 38, 227, 252, 287
BsiEI CGRYCG 2 cut(s) 39, 228
BsiHKAI GWGCWC 2 cut(s) 114, 358
BsiHKCI CYCGRG 2 cut(s) 113, 134
BsiSI CCGG 2 cut(s) 21, 253
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 1 cut(s) 22
BsmBI CGTCTC 1 cut(s) 22
BsmFI GGGAC 1 cut(s) 514
BsmI GAATGC 1 cut(s) 329
BsnI GGCC 4 cut(s) 38, 227, 252, 287
BsoBI CYCGRG 2 cut(s) 113, 134
Bsp1286I GDGCHC 2 cut(s) 114, 358
Bsp143I GATC 5 cut(s) 73, 257, 403, 421, 702
BspACI CCGC 4 cut(s) 82, 107, 156, 271
BspANI GGCC 4 cut(s) 38, 227, 252, 287
BspCNI CTCAG 1 cut(s) 605
BspFNI CGCG 2 cut(s) 84, 271
BspMAI CTGCAG 1 cut(s) 105
BspPI GGATC 3 cut(s) 81, 265, 411
BsrI ACTGG 1 cut(s) 157
BssECI CCNNGG 3 cut(s) 33, 253, 448
BssMI GATC 5 cut(s) 73, 257, 403, 421, 702
BssSI CACGAG 1 cut(s) 216
BssT1I CCWWGG 1 cut(s) 448
Bst2BI CACGAG 1 cut(s) 216
Bst4CI ACNGT 1 cut(s) 212
Bst6I CTCTTC 1 cut(s) 83
BstAPI GCANNNNNTGC 1 cut(s) 109
BstC8I GCNNGC 3 cut(s) 105, 273, 505
BstDEI CTNAG 3 cut(s) 543, 578, 592
BstENI CCTNNNNNAGG 1 cut(s) 387
BstF5I GGATG 1 cut(s) 637
BstFNI CGCG 2 cut(s) 84, 271
BstH2I RGCGCY 2 cut(s) 134, 244
BstHHI GCGC 2 cut(s) 133, 243
BstKTI GATC 5 cut(s) 76, 260, 406, 424, 705
BstMAI GTCTC 1 cut(s) 22
BstMBI GATC 5 cut(s) 73, 257, 403, 421, 702
BstMCI CGRYCG 2 cut(s) 39, 228
BstMWI GCNNNNNNNGC 4 cut(s) 109, 139, 249, 722
BstSCI CCNGG 1 cut(s) 252
BstSFI CTRYAG 1 cut(s) 101
BstSLI GKGCMC 1 cut(s) 358
BstUI CGCG 2 cut(s) 84, 271
BstV1I GCAGC 1 cut(s) 502
BstV2I GAAGAC 2 cut(s) 101, 201
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
BstZI CGGCCG 2 cut(s) 36, 225
BsuRI GGCC 4 cut(s) 38, 227, 252, 287
BtsCI GGATG 1 cut(s) 637
BtsI GCAGTG 1 cut(s) 125
BtsIMutI CAGTG 2 cut(s) 125, 600
Cac8I GCNNGC 3 cut(s) 105, 273, 505
CfoI GCGC 2 cut(s) 133, 243
Cfr13I GGNCC 1 cut(s) 250
CseI GACGC 1 cut(s) 50
Csp6I GTAC 1 cut(s) 147
CviAII CATG 1 cut(s) 627
CviQI GTAC 1 cut(s) 147
DdeI CTNAG 3 cut(s) 543, 578, 592
DpnI GATC 5 cut(s) 75, 259, 405, 423, 704
DpnII GATC 5 cut(s) 73, 257, 403, 421, 702
EaeI YGGCCR 2 cut(s) 36, 225
EagI CGGCCG 2 cut(s) 36, 225
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
EclXI CGGCCG 2 cut(s) 36, 225
Eco130I CCWWGG 1 cut(s) 448
Eco47III AGCGCT 1 cut(s) 132
Eco52I CGGCCG 2 cut(s) 36, 225
Eco57I CTGAAG 2 cut(s) 405, 551
Eco88I CYCGRG 2 cut(s) 113, 134
EcoNI CCTNNNNNAGG 1 cut(s) 387
EcoT14I CCWWGG 1 cut(s) 448
ErhI CCWWGG 1 cut(s) 448
Esp3I CGTCTC 1 cut(s) 22
FaeI CATG 1 cut(s) 630
FaqI GGGAC 1 cut(s) 514
FatI CATG 1 cut(s) 626
FauI CCCGC 1 cut(s) 264
FbaI TGATCA 1 cut(s) 702
Fnu4HI GCNGC 1 cut(s) 516
FokI GGATG 1 cut(s) 644
Fsp4HI GCNGC 1 cut(s) 516
FspBI CTAG 2 cut(s) 264, 347
GlaI GCGC 2 cut(s) 132, 242
GluI GCNGC 1 cut(s) 516
HaeII RGCGCY 2 cut(s) 134, 244
HaeIII GGCC 4 cut(s) 38, 227, 252, 287
HapII CCGG 2 cut(s) 21, 253
HgaI GACGC 1 cut(s) 50
HhaI GCGC 2 cut(s) 133, 243
Hin1II CATG 1 cut(s) 630
Hin6I GCGC 2 cut(s) 131, 241
HinP1I GCGC 2 cut(s) 131, 241
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HindIII AAGCTT 1 cut(s) 614
HinfI GANTC 3 cut(s) 62, 97, 137
HpaII CCGG 2 cut(s) 21, 253
HphI GGTGA 1 cut(s) 464
Hpy166II GTNNAC 2 cut(s) 232, 356
Hpy188I TCNGA 1 cut(s) 621
Hpy188III TCNNGA 3 cut(s) 315, 569, 730
Hpy8I GTNNAC 2 cut(s) 232, 356
Hpy99I CGWCG 3 cut(s) 44, 239, 293
HpyAV CCTTC 6 cut(s) 64, 314, 383, 601, 616, 736
HpyCH4III ACNGT 1 cut(s) 212
HpyCH4V TGCA 3 cut(s) 103, 356, 518
HpyF10VI GCNNNNNNNGC 4 cut(s) 109, 139, 249, 722
HpyF3I CTNAG 3 cut(s) 543, 578, 592
Hsp92II CATG 1 cut(s) 630
HspAI GCGC 2 cut(s) 131, 241
Ksp22I TGATCA 1 cut(s) 702
Kzo9I GATC 5 cut(s) 73, 257, 403, 421, 702
LmnI GCTCC 1 cut(s) 84
Lsp1109I GCAGC 1 cut(s) 502
LweI GCATC 1 cut(s) 668
MaeI CTAG 2 cut(s) 264, 347
MalI GATC 5 cut(s) 75, 259, 405, 423, 704
MboI GATC 5 cut(s) 73, 257, 403, 421, 702
MflI RGATCY 1 cut(s) 403
MhlI GDGCHC 2 cut(s) 114, 358
MluCI AATT 2 cut(s) 394, 548
MlyI GAGTC 2 cut(s) 91, 146
MroXI GAANNNNTTC 1 cut(s) 725
MseI TTAA 2 cut(s) 309, 534
MspI CCGG 2 cut(s) 21, 253
MspR9I CCNGG 1 cut(s) 254
Mva1269I GAATGC 1 cut(s) 329
MvnI CGCG 2 cut(s) 84, 271
MwoI GCNNNNNNNGC 4 cut(s) 109, 139, 249, 722
NciI CCSGG 1 cut(s) 254
NdeII GATC 5 cut(s) 73, 257, 403, 421, 702
NlaIII CATG 1 cut(s) 630
NmeAIII GCCGAG 1 cut(s) 14
PaeR7I CTCGAG 2 cut(s) 113, 134
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PctI GAATGC 1 cut(s) 329
PdmI GAANNNNTTC 1 cut(s) 725
PfeI GAWTC 1 cut(s) 62
PkrI GCNGC 1 cut(s) 517
PleI GAGTC 2 cut(s) 91, 145
PpsI GAGTC 2 cut(s) 91, 145
PspPI GGNCC 1 cut(s) 250
PspXI VCTCGAGB 2 cut(s) 113, 134
PstI CTGCAG 1 cut(s) 105
PsuI RGATCY 1 cut(s) 403
RsaI GTAC 1 cut(s) 148
RsaNI GTAC 1 cut(s) 147
SaqAI TTAA 2 cut(s) 309, 534
SatI GCNGC 1 cut(s) 516
Sau3AI GATC 5 cut(s) 73, 257, 403, 421, 702
Sau96I GGNCC 1 cut(s) 250
SchI GAGTC 2 cut(s) 91, 146
ScrFI CCNGG 1 cut(s) 254
SduI GDGCHC 2 cut(s) 114, 358
SfaNI GCATC 1 cut(s) 668
SfcI CTRYAG 1 cut(s) 101
Sfr274I CTCGAG 2 cut(s) 113, 134
SlaI CTCGAG 2 cut(s) 113, 134
SmlI CTYRAG 2 cut(s) 113, 134
SmoI CTYRAG 2 cut(s) 113, 134
SpeI ACTAGT 1 cut(s) 263
Sse9I AATT 2 cut(s) 394, 548
SsiI CCGC 4 cut(s) 82, 107, 156, 271
SspMI CTAG 2 cut(s) 264, 347
StyD4I CCNGG 1 cut(s) 252
StyI CCWWGG 1 cut(s) 448
TaaI ACNGT 1 cut(s) 212
TaqI TCGA 6 cut(s) 9, 26, 114, 135, 260, 314
TasI AATT 2 cut(s) 394, 548
TfiI GAWTC 1 cut(s) 62
Tru1I TTAA 2 cut(s) 309, 534
Tru9I TTAA 2 cut(s) 309, 534
TscAI CASTG 2 cut(s) 125, 600
TseI GCWGC 1 cut(s) 515
TspRI CASTG 2 cut(s) 125, 600
VneI GTGCAC 1 cut(s) 354
XagI CCTNNNNNAGG 1 cut(s) 387
XhoI CTCGAG 2 cut(s) 113, 134
XmnI GAANNNNTTC 1 cut(s) 725
XspI CTAG 2 cut(s) 264, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.