Rorug07G0302500

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0005332
Physical Location & Seq
Forward (+)
30080953 .. 30085306
4354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0302500.1

Sequence Viewer

Length: 888 bp
ATGACTTACCCCATTCTTTGCTCCGGTCATGACCGTTCCATTATGGATCTGTCTTACAGCCCAGATCCTTGTGGTGGTTTTCTTCTACTCAGTGCCAGTATGGATAGGCGGGCCATTCTGAGAGATGGAGAGAGTGGAGATTGGATTAGAACATTTGCTGAACAAACTTCTTCAGTATGGAATTGCTCTTTGAATATTACTGCAACTGCTTCAACGGCTGCAACTGCCTCTCATTGCAATGCCAAAGTTTGGGATCTGCAAACAGGAAAGTTGTTGCACATAATTCAAAGCCGGAATCTAGTTCCATCCTGTGCTTTCTCAAAGAATGGGCACAGTTTGCTAACAGGAGGACTCGGCTACACTATCCGTATATTTGATTTGGATCATCCACATGGAAAACCAGAAATCTTCCGGGAGTCAAATAAACCTCAGAGAGGATCAGTTCTTACTGCTGCATGGCTCCACAACGATCAGGTCATATTGAGTACGTGTAGTCTTCTACAGGGTGTCAGATTATGGGATCGAAGGAGTGGTAAGATACTCCGTACACTTGAGACTGAGTCAATCGTGAGAAGTTCAGAACTGAGTCAAGGTTATATCACCACTGCTGCTGGCTCTACGGTGACATTTTGGGATGAAACCAACTTTAGGTGCGTGAATCGCTACAAAATGCCATGCATGGTACAATCGGCTTCTTTGTATGGGAACAAGTTTATTGCAGGGGGAAATGATGGGCTTGTTCGTTTGTTTGATTTCCATACTGGTGAAGAGATTGAACAATCTAATATGGGTGACTTCGGAACCGTTTTATGTGTGCGTTTCTCGCCCGATGGAAAATCCTATGCCTCGGGATTTGAAGATGGCACTGTTATAATCAAGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

295

Amino Acids

32.54

Weight (kDa)

7.06

Isoelectric Point (pI)

49.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 4 - 233 1.2e-18 EIF3I
Beta-prop_WDR3_1st PF25173 7 - 127 5.5e-08 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 8 - 183 2.7e-17 WDR5 beta-propeller domain
WD40_Prp19 PF24814 8 - 127 5.3e-07 Prp19 WD40 domain
WD40_Gbeta PF25391 79 - 288 6.2e-09 G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd PF25047 99 - 192 3.9e-06 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 100 - 291 9.5e-21 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 151 - 281 6.3e-10 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 164 - 292 8.5e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 197 - 292 7.4e-06 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 198 - 292 2.5e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 199 - 292 3e-07 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 872
AccB7I CCANNNNNTGG 1 cut(s) 249
AciI CCGC 1 cut(s) 109
AclWI GGATC 6 cut(s) 54, 59, 261, 390, 445, 528
AcuI CTGAAG 1 cut(s) 156
AfaI GTAC 3 cut(s) 487, 547, 684
AfiI CCNNNNNNNGG 4 cut(s) 74, 249, 434, 648
AflIII ACRYGT 1 cut(s) 488
AgsI TTSAA 5 cut(s) 193, 213, 287, 776, 857
Alw26I GTCTC 1 cut(s) 548
AlwI GGATC 6 cut(s) 54, 59, 261, 390, 445, 528
Ama87I CYCGRG 1 cut(s) 847
AoxI GGCC 1 cut(s) 111
ApeKI GCWGC 3 cut(s) 218, 452, 608
AspS9I GGNCC 1 cut(s) 111
AsuC2I CCSGG 1 cut(s) 413
AsuHPI GGTGA 4 cut(s) 592, 634, 776, 803
AvaI CYCGRG 1 cut(s) 847
BaeGI GKGCMC 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 488
BbvI GCAGC 3 cut(s) 205, 439, 595
BccI CCATC 5 cut(s) 119, 313, 725, 824, 854
BceAI ACGGC 1 cut(s) 231
BcnI CCSGG 1 cut(s) 413
BcoDI GTCTC 1 cut(s) 548
BfaI CTAG 1 cut(s) 299
BfmI CTRYAG 1 cut(s) 500
BisI GCNGC 3 cut(s) 219, 453, 609
BlsI GCNGC 3 cut(s) 220, 454, 610
Bme1390I CCNGG 1 cut(s) 413
BmeT110I CYCGRG 1 cut(s) 847
BmgT120I GGNCC 1 cut(s) 111
BmiI GGNNCC 2 cut(s) 461, 802
BmrFI CCNGG 1 cut(s) 413
BpiI GAAGAC 1 cut(s) 488
BpuEI CTTGAG 1 cut(s) 572
BpuMI CCSGG 1 cut(s) 413
BsaAI YACGTR 1 cut(s) 489
BsaBI GATNNNNATC 1 cut(s) 381
BsaJI CCNNGG 1 cut(s) 846
BsaWI WCCGGW 1 cut(s) 23
Bsc4I CCNNNNNNNGG 4 cut(s) 74, 249, 434, 648
Bse1I ACTGG 2 cut(s) 96, 766
Bse3DI GCAATG 2 cut(s) 232, 244
Bse8I GATNNNNATC 1 cut(s) 381
BseDI CCNNGG 1 cut(s) 846
BseGI GGATG 3 cut(s) 305, 385, 640
BseJI GATNNNNATC 1 cut(s) 381
BseLI CCNNNNNNNGG 4 cut(s) 74, 249, 434, 648
BseMI GCAATG 2 cut(s) 232, 244
BseMII CTCAG 5 cut(s) 103, 110, 443, 549, 575
BseNI ACTGG 2 cut(s) 96, 766
BseSI GKGCMC 1 cut(s) 333
BseXI GCAGC 3 cut(s) 205, 439, 595
BshFI GGCC 1 cut(s) 113
BsiHKCI CYCGRG 1 cut(s) 847
BsiSI CCGG 3 cut(s) 24, 292, 412
BslI CCNNNNNNNGG 4 cut(s) 74, 249, 434, 648
BsmAI GTCTC 1 cut(s) 548
BsnI GGCC 1 cut(s) 113
BsoBI CYCGRG 1 cut(s) 847
Bsp1286I GDGCHC 1 cut(s) 333
Bsp143I GATC 7 cut(s) 46, 64, 253, 382, 437, 469, 520
BspACI CCGC 1 cut(s) 109
BspANI GGCC 1 cut(s) 113
BspCNI CTCAG 5 cut(s) 102, 111, 442, 550, 576
BspHI TCATGA 1 cut(s) 28
BspLI GGNNCC 2 cut(s) 461, 802
BspPI GGATC 6 cut(s) 54, 59, 261, 390, 445, 528
BsrDI GCAATG 2 cut(s) 232, 244
BsrI ACTGG 2 cut(s) 96, 766
BssECI CCNNGG 1 cut(s) 846
BssMI GATC 7 cut(s) 46, 64, 253, 382, 437, 469, 520
Bst4CI ACNGT 5 cut(s) 35, 335, 622, 805, 868
Bst6I CTCTTC 1 cut(s) 762
BstAPI GCANNNNNTGC 1 cut(s) 337
BstBAI YACGTR 1 cut(s) 489
BstC8I GCNNGC 2 cut(s) 111, 613
BstDEI CTNAG 5 cut(s) 89, 119, 429, 558, 584
BstENI CCTNNNNNAGG 1 cut(s) 432
BstF5I GGATG 3 cut(s) 305, 385, 640
BstKTI GATC 7 cut(s) 49, 67, 256, 385, 440, 472, 523
BstMAI GTCTC 1 cut(s) 548
BstMBI GATC 7 cut(s) 46, 64, 253, 382, 437, 469, 520
BstMWI GCNNNNNNNGC 5 cut(s) 215, 224, 337, 660, 823
BstSCI CCNGG 1 cut(s) 411
BstSFI CTRYAG 1 cut(s) 500
BstSLI GKGCMC 1 cut(s) 333
BstV1I GCAGC 3 cut(s) 205, 439, 595
BstV2I GAAGAC 1 cut(s) 488
BstX2I RGATCY 3 cut(s) 46, 64, 253
BstYI RGATCY 3 cut(s) 46, 64, 253
BsuRI GGCC 1 cut(s) 113
BtsCI GGATG 3 cut(s) 305, 385, 640
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 3 cut(s) 97, 603, 864
Cac8I GCNNGC 2 cut(s) 111, 613
CciI TCATGA 1 cut(s) 28
Cfr13I GGNCC 1 cut(s) 111
Csp6I GTAC 3 cut(s) 486, 546, 683
CviAII CATG 5 cut(s) 29, 392, 456, 675, 679
CviJI RGCY 9 cut(s) 60, 113, 218, 291, 357, 460, 615, 692, 736
CviKI_1 RGCY 9 cut(s) 60, 113, 218, 291, 357, 460, 615, 692, 736
CviQI GTAC 3 cut(s) 486, 546, 683
DdeI CTNAG 5 cut(s) 89, 119, 429, 558, 584
DpnI GATC 7 cut(s) 48, 66, 255, 384, 439, 471, 522
DpnII GATC 7 cut(s) 46, 64, 253, 382, 437, 469, 520
Eam1104I CTCTTC 1 cut(s) 762
EarI CTCTTC 1 cut(s) 762
Eco57I CTGAAG 1 cut(s) 156
Eco88I CYCGRG 1 cut(s) 847
EcoNI CCTNNNNNAGG 1 cut(s) 432
EcoT22I ATGCAT 1 cut(s) 680
FaeI CATG 5 cut(s) 32, 395, 459, 678, 682
FatI CATG 5 cut(s) 28, 391, 455, 674, 678
FauI CCCGC 1 cut(s) 102
Fnu4HI GCNGC 3 cut(s) 219, 453, 609
FokI GGATG 3 cut(s) 292, 372, 647
Fsp4HI GCNGC 3 cut(s) 219, 453, 609
FspBI CTAG 1 cut(s) 299
GluI GCNGC 3 cut(s) 219, 453, 609
HaeIII GGCC 1 cut(s) 113
HapII CCGG 3 cut(s) 24, 292, 412
Hin1II CATG 5 cut(s) 32, 395, 459, 678, 682
HinfI GANTC 6 cut(s) 295, 351, 416, 560, 586, 658
HpaII CCGG 3 cut(s) 24, 292, 412
HphI GGTGA 4 cut(s) 592, 634, 776, 803
Hpy166II GTNNAC 1 cut(s) 548
Hpy188I TCNGA 5 cut(s) 120, 432, 512, 580, 800
Hpy188III TCNNGA 4 cut(s) 29, 568, 849, 877
Hpy8I GTNNAC 1 cut(s) 548
HpyAV CCTTC 1 cut(s) 519
HpyCH4III ACNGT 5 cut(s) 35, 335, 622, 805, 868
HpyCH4IV ACGT 1 cut(s) 488
HpyCH4V TGCA 8 cut(s) 203, 221, 237, 259, 277, 455, 678, 719
HpyF10VI GCNNNNNNNGC 5 cut(s) 215, 224, 337, 660, 823
HpyF3I CTNAG 5 cut(s) 89, 119, 429, 558, 584
HpySE526I ACGT 1 cut(s) 488
Hsp92II CATG 5 cut(s) 32, 395, 459, 678, 682
Kzo9I GATC 7 cut(s) 46, 64, 253, 382, 437, 469, 520
LmnI GCTCC 2 cut(s) 26, 465
Lsp1109I GCAGC 3 cut(s) 205, 439, 595
MaeI CTAG 1 cut(s) 299
MaeII ACGT 1 cut(s) 488
MaeIII GTNAC 2 cut(s) 622, 791
MalI GATC 7 cut(s) 48, 66, 255, 384, 439, 471, 522
MboI GATC 7 cut(s) 46, 64, 253, 382, 437, 469, 520
MboII GAAGA 6 cut(s) 74, 162, 400, 488, 779, 869
MflI RGATCY 3 cut(s) 46, 64, 253
MhlI GDGCHC 1 cut(s) 333
MluCI AATT 2 cut(s) 181, 282
MlyI GAGTC 4 cut(s) 345, 425, 569, 595
MnlI CCTC 5 cut(s) 238, 341, 428, 438, 856
Mph1103I ATGCAT 1 cut(s) 680
MslI CAYNNNNRTG 3 cut(s) 237, 390, 762
MspI CCGG 3 cut(s) 24, 292, 412
MspR9I CCNGG 1 cut(s) 413
MwoI GCNNNNNNNGC 5 cut(s) 215, 224, 337, 660, 823
NciI CCSGG 1 cut(s) 413
NdeII GATC 7 cut(s) 46, 64, 253, 382, 437, 469, 520
NlaIII CATG 5 cut(s) 32, 395, 459, 678, 682
NlaIV GGNNCC 2 cut(s) 461, 802
NmeAIII GCCGAG 1 cut(s) 333
NmuCI GTSAC 2 cut(s) 622, 791
NsiI ATGCAT 1 cut(s) 680
PagI TCATGA 1 cut(s) 28
PfeI GAWTC 2 cut(s) 295, 658
PflFI GACNNNGTC 1 cut(s) 559
PflMI CCANNNNNTGG 1 cut(s) 249
PfoI TCCNGGA 1 cut(s) 411
PkrI GCNGC 3 cut(s) 220, 454, 610
PleI GAGTC 4 cut(s) 345, 424, 568, 594
PpsI GAGTC 4 cut(s) 345, 424, 568, 594
Ppu21I YACGTR 1 cut(s) 489
PsiI TTATAA 1 cut(s) 872
PspN4I GGNNCC 2 cut(s) 461, 802
PspPI GGNCC 1 cut(s) 111
PsuI RGATCY 3 cut(s) 46, 64, 253
PsyI GACNNNGTC 1 cut(s) 559
RsaI GTAC 3 cut(s) 487, 547, 684
RsaNI GTAC 3 cut(s) 486, 546, 683
RseI CAYNNNNRTG 3 cut(s) 237, 390, 762
SatI GCNGC 3 cut(s) 219, 453, 609
Sau3AI GATC 7 cut(s) 46, 64, 253, 382, 437, 469, 520
Sau96I GGNCC 1 cut(s) 111
SchI GAGTC 4 cut(s) 345, 425, 569, 595
ScrFI CCNGG 1 cut(s) 413
SduI GDGCHC 1 cut(s) 333
SetI ASST 5 cut(s) 430, 477, 491, 595, 653
SfcI CTRYAG 1 cut(s) 500
SmiMI CAYNNNNRTG 3 cut(s) 237, 390, 762
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
Sse9I AATT 2 cut(s) 181, 282
SsiI CCGC 1 cut(s) 109
SspI AATATT 1 cut(s) 196
SspMI CTAG 1 cut(s) 299
StyD4I CCNGG 1 cut(s) 411
TaaI ACNGT 5 cut(s) 35, 335, 622, 805, 868
TaiI ACGT 1 cut(s) 491
TaqI TCGA 1 cut(s) 523
TasI AATT 2 cut(s) 181, 282
TfiI GAWTC 2 cut(s) 295, 658
TscAI CASTG 3 cut(s) 97, 610, 871
TseFI GTSAC 2 cut(s) 622, 791
TseI GCWGC 3 cut(s) 218, 452, 608
Tsp45I GTSAC 2 cut(s) 622, 791
TspDTI ATGAA 1 cut(s) 651
TspGWI ACGGA 2 cut(s) 356, 533
TspRI CASTG 3 cut(s) 97, 610, 871
Tth111I GACNNNGTC 1 cut(s) 559
Van91I CCANNNNNTGG 1 cut(s) 249
XagI CCTNNNNNAGG 1 cut(s) 432
XspI CTAG 1 cut(s) 299
Zsp2I ATGCAT 1 cut(s) 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.