MD15G1383800.v1.1

Universal stress protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010262
Physical Location & Seq
Reverse (-)
47262613 .. 47266886
4274 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1383800.v1.1.491

Sequence Viewer

Length: 516 bp
ATGACCGGAAACCTCGGATGCGTGATCGTGGCCGTTGATGGCAGCGAGGAGAGCATGAACGCCTTGACCTGGGCCCTCGACAACCTCAAGCTCCGATCCTCTGCACCCGACTCCACCACCTCCGCTGGCCACTTCGTCGTCCTCCACGTCCAGTCTCCGCCCTCTATCGCAGCCGGCCTCAGTCCCCCGGCCATTCCCTTCGGTGGTCCCAACGATTTGGAGGTGCCGGCGTTCACGGCGGCGATCGAGGCGCACCAGAGGCGGATTACGGAGGCCATAATTGAACACGCTTTGAAAATTTGCTCCGAAAAGAAGGTGAATGTAAAAACCCAAGTGGTTGTTGGGGATCCTAAGGAGAAAATTGGTGAAGCTGTTGAGAATTTGCATGCTGATTTGCTTGTCATGGGGTCCAGGGCTTTTGGCCCAATTAAAAGGATGTTTCTGGGAAGTGTAAGCAACTTCTGTGCGAACCATGTGCAATGCCCAGTCATCATTGTTAAGGCCAAGACTACTTGA

Protein Analysis

172

Amino Acids

18.08

Weight (kDa)

6.58

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 8 - 167 1.7e-26 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 223
AciI CCGC 4 cut(s) 123, 158, 239, 262
AclWI GGATC 3 cut(s) 90, 341, 354
AcoI YGGCCR 3 cut(s) 30, 127, 189
AcsI RAATTY 2 cut(s) 297, 379
AfiI CCNNNNNNNGG 2 cut(s) 69, 203
AgsI TTSAA 2 cut(s) 284, 295
AjiI CACGTC 1 cut(s) 148
AjnI CCWGG 2 cut(s) 68, 410
AluBI AGCT 2 cut(s) 91, 371
AluI AGCT 2 cut(s) 91, 371
Alw26I GTCTC 1 cut(s) 159
AlwI GGATC 3 cut(s) 90, 341, 354
AoxI GGCC 8 cut(s) 30, 72, 127, 175, 189, 273, 421, 501
ApaI GGGCCC 1 cut(s) 76
ApeKI GCWGC 2 cut(s) 42, 170
ApoI RAATTY 2 cut(s) 297, 379
AspLEI GCGC 1 cut(s) 253
AspS9I GGNCC 5 cut(s) 72, 73, 206, 408, 422
AsuC2I CCSGG 1 cut(s) 188
AsuHPI GGTGA 2 cut(s) 328, 377
AvaII GGWCC 2 cut(s) 206, 408
AxyI CCTNAGG 1 cut(s) 351
BaeGI GKGCMC 1 cut(s) 76
BalI TGGCCA 1 cut(s) 129
BamHI GGATCC 1 cut(s) 346
BanI GGYRCC 1 cut(s) 223
BanII GRGCYC 1 cut(s) 76
BbvI GCAGC 2 cut(s) 54, 182
BccI CCATC 1 cut(s) 32
BceAI ACGGC 2 cut(s) 17, 252
BcgI CGANNNNNNTGC 2 cut(s) 457, 491
BciT130I CCWGG 2 cut(s) 70, 412
BcnI CCSGG 1 cut(s) 188
BcoDI GTCTC 1 cut(s) 159
BisI GCNGC 3 cut(s) 43, 171, 240
BlsI GCNGC 3 cut(s) 44, 172, 241
Bme1390I CCNGG 3 cut(s) 70, 188, 412
Bme18I GGWCC 2 cut(s) 206, 408
BmgBI CACGTC 1 cut(s) 148
BmgT120I GGNCC 5 cut(s) 72, 73, 206, 408, 422
BmiI GGNNCC 5 cut(s) 74, 208, 225, 348, 409
BmrFI CCNGG 3 cut(s) 70, 188, 412
BmrI ACTGGG 1 cut(s) 479
BmsI GCATC 1 cut(s) 8
BmuI ACTGGG 1 cut(s) 479
BpuEI CTTGAG 1 cut(s) 71
BpuMI CCSGG 1 cut(s) 188
BsaJI CCNNGG 4 cut(s) 13, 69, 186, 411
BsaWI WCCGGW 1 cut(s) 5
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 203
Bse118I RCCGGY 2 cut(s) 173, 226
Bse1I ACTGG 2 cut(s) 151, 485
Bse21I CCTNAGG 1 cut(s) 351
Bse3DI GCAATG 1 cut(s) 485
BseBI CCWGG 2 cut(s) 70, 412
BseDI CCNNGG 4 cut(s) 13, 69, 186, 411
BseGI GGATG 2 cut(s) 23, 441
BseLI CCNNNNNNNGG 2 cut(s) 69, 203
BseMI GCAATG 1 cut(s) 485
BseMII CTCAG 1 cut(s) 193
BseNI ACTGG 2 cut(s) 151, 485
BseRI GAGGAG 1 cut(s) 62
BseSI GKGCMC 1 cut(s) 76
BseXI GCAGC 2 cut(s) 54, 182
BsgI GTGCAG 1 cut(s) 87
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 8 cut(s) 32, 74, 129, 177, 191, 275, 423, 503
BshNI GGYRCC 1 cut(s) 223
BsiEI CGRYCG 1 cut(s) 246
BsiSI CCGG 4 cut(s) 6, 174, 188, 227
BslFI GGGAC 2 cut(s) 168, 192
BslI CCNNNNNNNGG 2 cut(s) 69, 203
BsmAI GTCTC 1 cut(s) 159
BsmFI GGGAC 2 cut(s) 168, 192
BsnI GGCC 8 cut(s) 32, 74, 129, 177, 191, 275, 423, 503
Bsp120I GGGCCC 1 cut(s) 72
Bsp1286I GDGCHC 1 cut(s) 76
Bsp143I GATC 4 cut(s) 24, 95, 243, 346
BspACI CCGC 4 cut(s) 123, 158, 239, 262
BspANI GGCC 8 cut(s) 32, 74, 129, 177, 191, 275, 423, 503
BspCNI CTCAG 1 cut(s) 192
BspLI GGNNCC 5 cut(s) 74, 208, 225, 348, 409
BspPI GGATC 3 cut(s) 90, 341, 354
BspT107I GGYRCC 1 cut(s) 223
BsrDI GCAATG 1 cut(s) 485
BsrFI RCCGGY 2 cut(s) 173, 226
BsrI ACTGG 2 cut(s) 151, 485
BssAI RCCGGY 2 cut(s) 173, 226
BssECI CCNNGG 4 cut(s) 13, 69, 186, 411
BssMI GATC 4 cut(s) 24, 95, 243, 346
Bst2UI CCWGG 2 cut(s) 70, 412
BstC8I GCNNGC 4 cut(s) 127, 175, 228, 387
BstDEI CTNAG 2 cut(s) 179, 351
BstF5I GGATG 2 cut(s) 23, 441
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 4 cut(s) 27, 98, 246, 349
BstMAI GTCTC 1 cut(s) 159
BstMBI GATC 4 cut(s) 24, 95, 243, 346
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 4 cut(s) 51, 236, 248, 259
BstNI CCWGG 2 cut(s) 70, 412
BstNSI RCATGY 1 cut(s) 389
BstSCI CCNGG 3 cut(s) 68, 186, 410
BstSLI GKGCMC 1 cut(s) 76
BstV1I GCAGC 2 cut(s) 54, 182
BstX2I RGATCY 1 cut(s) 346
BstXI CCANNNNNNTGG 1 cut(s) 217
BstYI RGATCY 1 cut(s) 346
Bsu36I CCTNAGG 1 cut(s) 351
BsuRI GGCC 8 cut(s) 32, 74, 129, 177, 191, 275, 423, 503
BtrI CACGTC 1 cut(s) 148
BtsCI GGATG 2 cut(s) 23, 441
Cac8I GCNNGC 4 cut(s) 127, 175, 228, 387
CfoI GCGC 1 cut(s) 253
Cfr10I RCCGGY 2 cut(s) 173, 226
Cfr13I GGNCC 5 cut(s) 72, 73, 206, 408, 422
CviAII CATG 4 cut(s) 55, 386, 403, 473
DdeI CTNAG 2 cut(s) 179, 351
DpnI GATC 4 cut(s) 26, 97, 245, 348
DpnII GATC 4 cut(s) 24, 95, 243, 346
EaeI YGGCCR 3 cut(s) 30, 127, 189
EciI GGCGGA 2 cut(s) 147, 277
Eco24I GRGCYC 1 cut(s) 76
Eco47I GGWCC 2 cut(s) 206, 408
Eco81I CCTNAGG 1 cut(s) 351
EcoO109I RGGNCCY 1 cut(s) 73
EcoRII CCWGG 2 cut(s) 68, 410
EcoT38I GRGCYC 1 cut(s) 76
FaeI CATG 4 cut(s) 58, 389, 406, 476
FaiI YATR 5 cut(s) 56, 278, 387, 404, 474
FaqI GGGAC 2 cut(s) 168, 192
FatI CATG 4 cut(s) 54, 385, 402, 472
Fnu4HI GCNGC 3 cut(s) 43, 171, 240
FokI GGATG 2 cut(s) 30, 448
FriOI GRGCYC 1 cut(s) 76
Fsp4HI GCNGC 3 cut(s) 43, 171, 240
GlaI GCGC 1 cut(s) 252
GluI GCNGC 3 cut(s) 43, 171, 240
HaeIII GGCC 8 cut(s) 32, 74, 129, 177, 191, 275, 423, 503
HapII CCGG 4 cut(s) 6, 174, 188, 227
HhaI GCGC 1 cut(s) 253
Hin1II CATG 4 cut(s) 58, 389, 406, 476
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HinfI GANTC 1 cut(s) 110
HpaII CCGG 4 cut(s) 6, 174, 188, 227
HphI GGTGA 2 cut(s) 328, 377
Hpy166II GTNNAC 1 cut(s) 234
Hpy188I TCNGA 3 cut(s) 17, 95, 307
Hpy8I GTNNAC 1 cut(s) 234
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 2 cut(s) 208, 307
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 3 cut(s) 104, 385, 478
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 236, 248, 259
HpyF3I CTNAG 2 cut(s) 179, 351
HpySE526I ACGT 1 cut(s) 147
Hsp92II CATG 4 cut(s) 58, 389, 406, 476
HspAI GCGC 1 cut(s) 251
KroI GCCGGC 2 cut(s) 173, 226
KroNI GCCGGC 2 cut(s) 175, 228
Kzo9I GATC 4 cut(s) 24, 95, 243, 346
LmnI GCTCC 2 cut(s) 96, 308
Lsp1109I GCAGC 2 cut(s) 54, 182
LweI GCATC 1 cut(s) 8
MaeII ACGT 1 cut(s) 147
MalI GATC 4 cut(s) 26, 97, 245, 348
MboI GATC 4 cut(s) 24, 95, 243, 346
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 1 cut(s) 76
MlsI TGGCCA 1 cut(s) 129
MluCI AATT 5 cut(s) 279, 297, 360, 379, 426
MluNI TGGCCA 1 cut(s) 129
MlyI GAGTC 1 cut(s) 104
Mox20I TGGCCA 1 cut(s) 129
MroNI GCCGGC 2 cut(s) 173, 226
MscI TGGCCA 1 cut(s) 129
MseI TTAA 2 cut(s) 429, 498
Msp20I TGGCCA 1 cut(s) 129
MspA1I CMGCKG 1 cut(s) 125
MspI CCGG 4 cut(s) 6, 174, 188, 227
MspR9I CCNGG 3 cut(s) 70, 188, 412
MvaI CCWGG 2 cut(s) 70, 412
MwoI GCNNNNNNNGC 4 cut(s) 51, 236, 248, 259
NaeI GCCGGC 2 cut(s) 175, 228
NciI CCSGG 1 cut(s) 188
NdeII GATC 4 cut(s) 24, 95, 243, 346
NgoMIV GCCGGC 2 cut(s) 173, 226
NlaIII CATG 4 cut(s) 58, 389, 406, 476
NlaIV GGNNCC 5 cut(s) 74, 208, 225, 348, 409
NspI RCATGY 1 cut(s) 389
PaeI GCATGC 1 cut(s) 389
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PdiI GCCGGC 2 cut(s) 175, 228
PkrI GCNGC 3 cut(s) 44, 172, 241
Ple19I CGATCG 1 cut(s) 246
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
Psp6I CCWGG 2 cut(s) 68, 410
PspGI CCWGG 2 cut(s) 68, 410
PspN4I GGNNCC 5 cut(s) 74, 208, 225, 348, 409
PspOMI GGGCCC 1 cut(s) 72
PspPI GGNCC 5 cut(s) 72, 73, 206, 408, 422
PsuI RGATCY 1 cut(s) 346
PvuI CGATCG 1 cut(s) 246
SaqAI TTAA 2 cut(s) 429, 498
SatI GCNGC 3 cut(s) 43, 171, 240
Sau3AI GATC 4 cut(s) 24, 95, 243, 346
Sau96I GGNCC 5 cut(s) 72, 73, 206, 408, 422
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 3 cut(s) 70, 188, 412
SduI GDGCHC 1 cut(s) 76
SetI ASST 9 cut(s) 15, 71, 87, 93, 122, 150, 225, 318, 373
SfaNI GCATC 1 cut(s) 8
SinI GGWCC 2 cut(s) 206, 408
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
SphI GCATGC 1 cut(s) 389
Sse9I AATT 5 cut(s) 279, 297, 360, 379, 426
SsiI CCGC 4 cut(s) 123, 158, 239, 262
StyD4I CCNGG 3 cut(s) 68, 186, 410
TaiI ACGT 1 cut(s) 150
TaqI TCGA 2 cut(s) 78, 246
TasI AATT 5 cut(s) 279, 297, 360, 379, 426
TauI GCSGC 1 cut(s) 242
Tru1I TTAA 2 cut(s) 429, 498
Tru9I TTAA 2 cut(s) 429, 498
TseI GCWGC 2 cut(s) 42, 170
TspDTI ATGAA 1 cut(s) 71
TspGWI ACGGA 1 cut(s) 284
VpaK11BI GGWCC 2 cut(s) 206, 408
XapI RAATTY 2 cut(s) 297, 379
XceI RCATGY 1 cut(s) 389
XcmI CCANNNNNNNNNTGG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.