MD15G1404200.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006600
Physical Location & Seq
Reverse (-)
50513327 .. 50516569
3243 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1404200.v1.1.491

Sequence Viewer

Length: 2181 bp
ATGCCACCATCTTATTCTCGTTTCATCCCAAGAGGCCTTTCTATATCTCAAATCCAAAAATTCATACCTAAAAGCTGGAAGGAAGGAACGAAACAATTTGGGAACTCATCTGCTTTATGTATTTCGGAAACAGGTTCAGCTGTTGATGATTCTATGATAGATTCTCTTCTTACATCCATCAAGAATTATGCAAGCCAAGGAAATTTATCAAAAGCATTCAAAACTGTCTCTCTTGTCCAGCTCTACACCTCTTCTCCTACTTGTGACCTTATCTTACATTCCATCTCTTCTGTTATCTTATCTTGCGCCAACCATAAAACATTACCGCAGGGTGAACAGCTTCATGCACATATTGTCAGATTGGGGTCTGAACGACACCCCATTTTGGTTCCCAAGCTGGTTACATTTTACTCAAGCTTTAATCTCCATGCTGAGGCTCGTATCATTATTGAGAATTCAAATATATTGCATCCTCTACCTTGGAATGTGCTCATCTCTTCATATGTTAAAAATGAACTTGTGGATGAGGCTCTCTCTACATATAAACAAATGGTCAACAAGGGGATTAGGCCAGATTGTTTCACTTACCCATCTGTTCTCAAGGCTTGTGGTGAAAAATTGGATATAAGTTTTGGCAGGGAGGTACATAAGTCCATTGATGCTCATTGTCAAGAGTGGAATTTATTTGTGCATAATTCATTGGTCTCCATGTATGGGAAATTTGGGCTTCTCAACATTGCTCGTCACTTATTCGACAAAATGCCCACAAGGGATGCAATTTCTTGGAATGCAATGATCTCTGGTTATGCCTCCAAGGGCATGTGGACGGATGCATTCGAGTTGTTTGGAAACATGCAGATGGAGGGTATTGGAGTTAATATCTTACTTTGGAATACAATTGCTGGTGGGTGCTTGAGGACAGGTAATTTCAGGGGCGCTCTTGAGTTGCTTTCTCAAATGATAAGTTGTGGTATTTTATTGGACTCAGTAGCATTGACGATTGGTTTGAGTGCTTGTTCCCACAATGGAGCCATTAAATTAGGGAAGGAAATTCATGGTTTTGCGATTCGTAGTGGTTGTGATGGGTATGATAATGTTAATAATGCATTGATTACATTGTATTCTAGGTGCAAAGACCTTAGGAAAGCCTATACTTTGTTTGAATTGATAGAAGATAGGAGTATAATTACTTGGAACTCCATGCTTTCTGGTTATTCTCACATGGATCGAGCTGAGGAAGCTTCATTCTTATTTAGGGAGATGTTGTGTTCAGGAATTGAACCTAACTATGTTACTATTGCCAGCATCCTTCCTCTCTGTGCTCGGGTAGCAAACCTTCAACATGGGAAGGAGTTCCACTGCTACATTACAAAACGTGTAGTGTTTGATGATTGTTTATTGCTACGGAATGCCCTTGTGGACATGTATGCCAGATCGGGCAAGGTTTTACAGGCGAAAAGAGTATTTGATTCAATGTCGAAGAGGGATGAAGTAACTTATACTTCCATGATTGCAGGATATGGAGTCCAGGGTGAAGGAAAAGCTGCACTGAAGTTATTCGAAGAGATGAACTTGTTACACATAAAACCAGATCATGTAACTATGGTTTCAATTTTATCAGCTTGTAGCCATTCCGGTCTAGTAATCCAAGGCCAAATGCTATTTGAAAAGATGCTGAGTGTATACGGTATAACTCCCAATTTGGAGCACTATGCCTGCATGGTTGATCTCTTTGGGAGGGCCGGTCTACTAAACAAAGCAAAGGAGATTATCACAAGGATGCCTTACAAGCCAACTTCTGCTATGTGGGCTACACTTATTGGAGCATGTCGGATCCATGGAAATATGGAGATAGGGGAATGGGCAGCCGGGAAGCTGTTAGAAATGAGACCGGAAAATTCTGGTTACTATGTGTTAATTGCTAACATGTATGCTGCTGCTGGTTGTTGGAACAACCTAGCGAGAGTGAGGACTTTCATGAGGGACTTGGGTGTGAGGAAGGATCCTGGCTGTGCTTGGGTTGATGTGGGCGATGGATTTTCACCCTTTTTGGTGGGGGACACAAATCGGCTGAGAGATGAAATTTACCTTTTGTTGGATGGCTTAACAGAGTTAATGAAAGATACTGATTCTGTTGTCAATGGGGATATAAGTTCAGGTTATGATGTTTTTGAGGATTAG

Protein Analysis

727

Amino Acids

80.74

Weight (kDa)

6.87

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 161 - 203 4e-11 PPR repeat family
PPR PF01535 161 - 189 6.8e-07 PPR repeat
PPR_2 PF13041 258 - 293 4e-07 PPR repeat family
PPR PF01535 260 - 290 3.4e-08 PPR repeat
PPR_2 PF13041 394 - 440 7.9e-09 PPR repeat family
PPR PF01535 396 - 426 5.6e-07 PPR repeat
PPR_2 PF13041 496 - 543 1.1e-10 PPR repeat family
PPR PF01535 498 - 523 3.9e-06 PPR repeat
E_motif PF20431 613 - 675 5.4e-22 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 1683, 1747
AciI CCGC 1 cut(s) 326
AclWI GGATC 5 cut(s) 1233, 1828, 1841, 1997, 2010
AcsI RAATTY 8 cut(s) 59, 202, 454, 679, 719, 1050, 1897, 2082
AcuI CTGAAG 1 cut(s) 1571
AfaI GTAC 1 cut(s) 645
AfiI CCNNNNNNNGG 4 cut(s) 385, 433, 714, 2095
AflIII ACRYGT 3 cut(s) 1375, 1422, 1926
AgsI TTSAA 8 cut(s) 220, 459, 1163, 1280, 1340, 1473, 1611, 1667
AjnI CCWGG 2 cut(s) 1527, 2005
AjuI GAANNNNNNNTTGG 2 cut(s) 22, 54
Alw21I GWGCWC 3 cut(s) 492, 1324, 1710
Alw26I GTCTC 3 cut(s) 232, 709, 1882
AlwI GGATC 5 cut(s) 1233, 1828, 1841, 1997, 2010
Ama87I CYCGRG 1 cut(s) 1323
AoxI GGCC 4 cut(s) 34, 569, 1651, 1740
ApeKI GCWGC 4 cut(s) 1544, 1865, 1934, 1937
ApoI RAATTY 8 cut(s) 59, 202, 454, 679, 719, 1050, 1897, 2082
ArsI GACNNNNNNTTYG 2 cut(s) 988, 1020
Asp700I GAANNNNTTC 3 cut(s) 339, 1352, 1556
AspLEI GCGC 2 cut(s) 308, 938
AspS9I GGNCC 1 cut(s) 1740
AsuC2I CCSGG 1 cut(s) 1870
AsuHPI GGTGA 4 cut(s) 344, 623, 1544, 2034
AsuII TTCGAA 1 cut(s) 1560
AvaI CYCGRG 1 cut(s) 1323
AxyI CCTNAGG 1 cut(s) 1139
BaeI ACNNNNGTAYC 2 cut(s) 635, 668
BamHI GGATCC 2 cut(s) 1833, 2002
BarI GAAGNNNNNNTAC 2 cut(s) 1320, 1352
Bbv12I GWGCWC 3 cut(s) 492, 1324, 1710
BbvCI CCTCAGC 2 cut(s) 432, 1233
BbvI GCAGC 4 cut(s) 1531, 1877, 1921, 1924
BccI CCATC 8 cut(s) 16, 185, 290, 598, 853, 1076, 2027, 2093
BciT130I CCWGG 2 cut(s) 1529, 2007
BcnI CCSGG 1 cut(s) 1870
BcoDI GTCTC 3 cut(s) 232, 709, 1882
BfaI CTAG 3 cut(s) 1125, 1640, 1958
BfoI RGCGCY 1 cut(s) 939
BisI GCNGC 4 cut(s) 1545, 1866, 1935, 1938
BlsI GCNGC 4 cut(s) 1546, 1867, 1936, 1939
Bme1390I CCNGG 3 cut(s) 1529, 1870, 2007
BmeT110I CYCGRG 1 cut(s) 1323
BmgT120I GGNCC 1 cut(s) 1740
BmiI GGNNCC 4 cut(s) 390, 1030, 1835, 2004
BmrFI CCNGG 3 cut(s) 1529, 1870, 2007
BmsI GCATC 7 cut(s) 478, 649, 763, 820, 1314, 1662, 1770
BplI GAGNNNNNCTC 2 cut(s) 518, 550
Bpu10I CCTNAGC 2 cut(s) 432, 1233
Bpu14I TTCGAA 1 cut(s) 1560
BpuEI CTTGAG 4 cut(s) 397, 584, 934, 962
BpuMI CCSGG 1 cut(s) 1870
BsaI GGTCTC 2 cut(s) 709, 1882
BsaJI CCNNGG 6 cut(s) 196, 479, 813, 1528, 1648, 1837
BsaWI WCCGGW 2 cut(s) 1634, 1891
BsaXI ACNNNNNCTCC 6 cut(s) 238, 268, 1250, 1280, 1729, 1759
Bsc4I CCNNNNNNNGG 4 cut(s) 385, 433, 714, 2095
Bse118I RCCGGY 1 cut(s) 1742
Bse21I CCTNAGG 1 cut(s) 1139
Bse3DI GCAATG 2 cut(s) 735, 798
BseBI CCWGG 2 cut(s) 1529, 2007
BseDI CCNNGG 6 cut(s) 196, 479, 813, 1528, 1648, 1837
BseLI CCNNNNNNNGG 4 cut(s) 385, 433, 714, 2095
BseMI GCAATG 2 cut(s) 735, 798
BseMII CTCAG 5 cut(s) 423, 999, 1224, 1667, 2063
BseXI GCAGC 4 cut(s) 1531, 1877, 1921, 1924
BsgI GTGCAG 1 cut(s) 1530
BshFI GGCC 4 cut(s) 36, 571, 1653, 1742
BsiHKAI GWGCWC 3 cut(s) 492, 1324, 1710
BsiHKCI CYCGRG 1 cut(s) 1323
BsiSI CCGG 4 cut(s) 1635, 1743, 1869, 1892
BslFI GGGAC 2 cut(s) 1997, 2072
BslI CCNNNNNNNGG 4 cut(s) 385, 433, 714, 2095
BsmAI GTCTC 3 cut(s) 232, 709, 1882
BsmFI GGGAC 2 cut(s) 1997, 2072
BsmI GAATGC 4 cut(s) 215, 793, 833, 1414
BsnI GGCC 4 cut(s) 36, 571, 1653, 1742
Bso31I GGTCTC 2 cut(s) 709, 1882
BsoBI CYCGRG 1 cut(s) 1323
Bsp119I TTCGAA 1 cut(s) 1560
Bsp1286I GDGCHC 3 cut(s) 492, 1324, 1710
Bsp143I GATC 7 cut(s) 795, 1225, 1433, 1591, 1726, 1833, 2002
Bsp19I CCATGG 1 cut(s) 1837
BspACI CCGC 1 cut(s) 326
BspANI GGCC 4 cut(s) 36, 571, 1653, 1742
BspCNI CTCAG 5 cut(s) 424, 998, 1225, 1668, 2064
BspHI TCATGA 1 cut(s) 1977
BspLI GGNNCC 4 cut(s) 390, 1030, 1835, 2004
BspPI GGATC 5 cut(s) 1233, 1828, 1841, 1997, 2010
BspT104I TTCGAA 1 cut(s) 1560
BspTNI GGTCTC 2 cut(s) 709, 1882
BsrDI GCAATG 2 cut(s) 735, 798
BsrFI RCCGGY 1 cut(s) 1742
BssAI RCCGGY 1 cut(s) 1742
BssECI CCNNGG 6 cut(s) 196, 479, 813, 1528, 1648, 1837
BssMI GATC 7 cut(s) 795, 1225, 1433, 1591, 1726, 1833, 2002
BssNAI GTATAC 1 cut(s) 1684
BssT1I CCWWGG 5 cut(s) 196, 479, 813, 1648, 1837
Bst1107I GTATAC 1 cut(s) 1684
Bst2UI CCWGG 2 cut(s) 1529, 2007
Bst4CI ACNGT 2 cut(s) 226, 1688
Bst6I CTCTTC 6 cut(s) 171, 256, 292, 502, 1475, 1557
BstBI TTCGAA 1 cut(s) 1560
BstC8I GCNNGC 3 cut(s) 193, 1303, 1717
BstDEI CTNAG 6 cut(s) 432, 985, 1139, 1233, 1676, 2072
BstDSI CCRYGG 1 cut(s) 1837
BstH2I RGCGCY 1 cut(s) 939
BstHHI GCGC 2 cut(s) 308, 938
BstKTI GATC 7 cut(s) 798, 1228, 1436, 1594, 1729, 1836, 2005
BstMAI GTCTC 3 cut(s) 232, 709, 1882
BstMBI GATC 7 cut(s) 795, 1225, 1433, 1591, 1726, 1833, 2002
BstMWI GCNNNNNNNGC 4 cut(s) 1238, 1328, 1789, 1808
BstNI CCWGG 2 cut(s) 1529, 2007
BstNSI RCATGY 5 cut(s) 823, 856, 1426, 1830, 1930
BstSCI CCNGG 3 cut(s) 1527, 1868, 2005
BstV1I GCAGC 4 cut(s) 1531, 1877, 1921, 1924
BstX2I RGATCY 2 cut(s) 1833, 2002
BstYI RGATCY 2 cut(s) 1833, 2002
BstZ17I GTATAC 1 cut(s) 1684
Bsu36I CCTNAGG 1 cut(s) 1139
BsuRI GGCC 4 cut(s) 36, 571, 1653, 1742
BtgI CCRYGG 1 cut(s) 1837
BtgZI GCGATG 1 cut(s) 2046
BtsI GCAGTG 1 cut(s) 1357
BtsIMutI CAGTG 2 cut(s) 1357, 1547
Cac8I GCNNGC 3 cut(s) 193, 1303, 1717
CciI TCATGA 1 cut(s) 1977
CfoI GCGC 2 cut(s) 308, 938
Cfr10I RCCGGY 1 cut(s) 1742
Cfr13I GGNCC 1 cut(s) 1740
Csp6I GTAC 1 cut(s) 644
CspCI CAANNNNNGTGG 2 cut(s) 589, 624
CviQI GTAC 1 cut(s) 644
DdeI CTNAG 6 cut(s) 432, 985, 1139, 1233, 1676, 2072
DpnI GATC 7 cut(s) 797, 1227, 1435, 1593, 1728, 1835, 2004
DpnII GATC 7 cut(s) 795, 1225, 1433, 1591, 1726, 1833, 2002
Eam1104I CTCTTC 6 cut(s) 171, 256, 292, 502, 1475, 1557
EarI CTCTTC 6 cut(s) 171, 256, 292, 502, 1475, 1557
Eco130I CCWWGG 5 cut(s) 196, 479, 813, 1648, 1837
Eco147I AGGCCT 1 cut(s) 36
Eco31I GGTCTC 2 cut(s) 709, 1882
Eco57I CTGAAG 1 cut(s) 1571
Eco81I CCTNAGG 1 cut(s) 1139
Eco88I CYCGRG 1 cut(s) 1323
EcoRI GAATTC 1 cut(s) 454
EcoRII CCWGG 2 cut(s) 1527, 2005
EcoT14I CCWWGG 5 cut(s) 196, 479, 813, 1648, 1837
EcoT22I ATGCAT 2 cut(s) 835, 1108
ErhI CCWWGG 5 cut(s) 196, 479, 813, 1648, 1837
FalI AAGNNNNNCTT 2 cut(s) 1768, 1800
FaqI GGGAC 2 cut(s) 1997, 2072
FauNDI CATATG 1 cut(s) 502
FblI GTMKAC 2 cut(s) 1683, 1747
Fnu4HI GCNGC 4 cut(s) 1545, 1866, 1935, 1938
Fsp4HI GCNGC 4 cut(s) 1545, 1866, 1935, 1938
FspBI CTAG 3 cut(s) 1125, 1640, 1958
GlaI GCGC 2 cut(s) 307, 937
GluI GCNGC 4 cut(s) 1545, 1866, 1935, 1938
HaeII RGCGCY 1 cut(s) 939
HaeIII GGCC 4 cut(s) 36, 571, 1653, 1742
HapII CCGG 4 cut(s) 1635, 1743, 1869, 1892
HhaI GCGC 2 cut(s) 308, 938
Hin6I GCGC 2 cut(s) 306, 936
HinP1I GCGC 2 cut(s) 306, 936
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HindIII AAGCTT 2 cut(s) 415, 1239
HinfI GANTC 7 cut(s) 149, 161, 983, 1066, 1469, 1524, 2129
HpaII CCGG 4 cut(s) 1635, 1743, 1869, 1892
HphI GGTGA 4 cut(s) 344, 623, 1544, 2034
Hpy166II GTNNAC 6 cut(s) 335, 556, 825, 1420, 1684, 1748
Hpy188I TCNGA 4 cut(s) 127, 359, 370, 1833
Hpy188III TCNNGA 5 cut(s) 181, 671, 941, 1272, 1978
Hpy8I GTNNAC 6 cut(s) 335, 556, 825, 1420, 1684, 1748
HpyAV CCTTC 8 cut(s) 73, 77, 1039, 1319, 1342, 1346, 1529, 1993
HpyCH4III ACNGT 2 cut(s) 226, 1688
HpyCH4IV ACGT 1 cut(s) 1375
HpyF10VI GCNNNNNNNGC 4 cut(s) 1238, 1328, 1789, 1808
HpyF3I CTNAG 6 cut(s) 432, 985, 1139, 1233, 1676, 2072
HpySE526I ACGT 1 cut(s) 1375
HspAI GCGC 2 cut(s) 306, 936
Kzo9I GATC 7 cut(s) 795, 1225, 1433, 1591, 1726, 1833, 2002
LmnI GCTCC 3 cut(s) 1028, 1705, 1823
Lsp1109I GCAGC 4 cut(s) 1531, 1877, 1921, 1924
LweI GCATC 7 cut(s) 478, 649, 763, 820, 1314, 1662, 1770
MaeI CTAG 3 cut(s) 1125, 1640, 1958
MaeII ACGT 1 cut(s) 1375
MaeIII GTNAC 8 cut(s) 263, 400, 743, 1291, 1492, 1575, 1597, 1904
MalI GATC 7 cut(s) 797, 1227, 1435, 1593, 1728, 1835, 2004
MboI GATC 7 cut(s) 795, 1225, 1433, 1591, 1726, 1833, 2002
MboII GAAGA 7 cut(s) 158, 243, 279, 489, 1184, 1492, 1574
MfeI CAATTG 1 cut(s) 897
MflI RGATCY 2 cut(s) 1833, 2002
MhlI GDGCHC 3 cut(s) 492, 1324, 1710
MlyI GAGTC 2 cut(s) 977, 1533
MmeI TCCRAC 3 cut(s) 1811, 1928, 2076
Mph1103I ATGCAT 2 cut(s) 835, 1108
MroXI GAANNNNTTC 3 cut(s) 339, 1352, 1556
MseI TTAA 8 cut(s) 420, 507, 876, 1035, 1098, 1916, 2105, 2114
MslI CAYNNNNRTG 2 cut(s) 857, 1778
MspA1I CMGCKG 1 cut(s) 140
MspI CCGG 4 cut(s) 1635, 1743, 1869, 1892
MspR9I CCNGG 3 cut(s) 1529, 1870, 2007
MunI CAATTG 1 cut(s) 897
Mva1269I GAATGC 4 cut(s) 215, 793, 833, 1414
MvaI CCWGG 2 cut(s) 1529, 2007
MwoI GCNNNNNNNGC 4 cut(s) 1238, 1328, 1789, 1808
NciI CCSGG 1 cut(s) 1870
NcoI CCATGG 1 cut(s) 1837
NdeI CATATG 1 cut(s) 502
NdeII GATC 7 cut(s) 795, 1225, 1433, 1591, 1726, 1833, 2002
NlaIV GGNNCC 4 cut(s) 390, 1030, 1835, 2004
NmuCI GTSAC 2 cut(s) 263, 743
NsiI ATGCAT 2 cut(s) 835, 1108
NspI RCATGY 5 cut(s) 823, 856, 1426, 1830, 1930
NspV TTCGAA 1 cut(s) 1560
PagI TCATGA 1 cut(s) 1977
PceI AGGCCT 1 cut(s) 36
PciI ACATGT 2 cut(s) 1422, 1926
PctI GAATGC 4 cut(s) 215, 793, 833, 1414
PdmI GAANNNNTTC 3 cut(s) 339, 1352, 1556
PfeI GAWTC 5 cut(s) 149, 161, 1066, 1469, 2129
PkrI GCNGC 4 cut(s) 1546, 1867, 1936, 1939
PleI GAGTC 2 cut(s) 977, 1532
PpsI GAGTC 2 cut(s) 977, 1532
PscI ACATGT 2 cut(s) 1422, 1926
Psp6I CCWGG 2 cut(s) 1527, 2005
PspGI CCWGG 2 cut(s) 1527, 2005
PspN4I GGNNCC 4 cut(s) 390, 1030, 1835, 2004
PspPI GGNCC 1 cut(s) 1740
PsuI RGATCY 2 cut(s) 1833, 2002
PvuII CAGCTG 1 cut(s) 140
RsaI GTAC 1 cut(s) 645
RsaNI GTAC 1 cut(s) 644
RseI CAYNNNNRTG 2 cut(s) 857, 1778
SaqAI TTAA 8 cut(s) 420, 507, 876, 1035, 1098, 1916, 2105, 2114
SatI GCNGC 4 cut(s) 1545, 1866, 1935, 1938
Sau3AI GATC 7 cut(s) 795, 1225, 1433, 1591, 1726, 1833, 2002
Sau96I GGNCC 1 cut(s) 1740
SchI GAGTC 2 cut(s) 977, 1533
ScrFI CCNGG 3 cut(s) 1529, 1870, 2007
SduI GDGCHC 3 cut(s) 492, 1324, 1710
SfaNI GCATC 7 cut(s) 478, 649, 763, 820, 1314, 1662, 1770
SfuI TTCGAA 1 cut(s) 1560
SmiMI CAYNNNNRTG 2 cut(s) 857, 1778
SmlI CTYRAG 4 cut(s) 412, 599, 913, 941
SmoI CTYRAG 4 cut(s) 412, 599, 913, 941
SseBI AGGCCT 1 cut(s) 36
SsiI CCGC 1 cut(s) 326
SspMI CTAG 3 cut(s) 1125, 1640, 1958
StuI AGGCCT 1 cut(s) 36
StyD4I CCNGG 3 cut(s) 1527, 1868, 2005
StyI CCWWGG 5 cut(s) 196, 479, 813, 1648, 1837
TaaI ACNGT 2 cut(s) 226, 1688
TaiI ACGT 1 cut(s) 1378
TaqI TCGA 5 cut(s) 753, 837, 1228, 1478, 1560
TfiI GAWTC 5 cut(s) 149, 161, 1066, 1469, 2129
Tru1I TTAA 8 cut(s) 420, 507, 876, 1035, 1098, 1916, 2105, 2114
Tru9I TTAA 8 cut(s) 420, 507, 876, 1035, 1098, 1916, 2105, 2114
TscAI CASTG 2 cut(s) 1364, 1554
TseFI GTSAC 2 cut(s) 263, 743
TseI GCWGC 4 cut(s) 1544, 1865, 1934, 1937
Tsp45I GTSAC 2 cut(s) 263, 743
TspGWI ACGGA 2 cut(s) 842, 1420
TspRI CASTG 2 cut(s) 1364, 1554
XapI RAATTY 8 cut(s) 59, 202, 454, 679, 719, 1050, 1897, 2082
XceI RCATGY 5 cut(s) 823, 856, 1426, 1830, 1930
XmiI GTMKAC 2 cut(s) 1683, 1747
XmnI GAANNNNTTC 3 cut(s) 339, 1352, 1556
XspI CTAG 3 cut(s) 1125, 1640, 1958
Zsp2I ATGCAT 2 cut(s) 835, 1108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.