MD15G1431000.v1.1

Ubiquitin-like domain-containing CTD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009260
Physical Location & Seq
Reverse (-)
53109904 .. 53113301
3398 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1431000.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGGCAGGAGTAGCAGGTGCAGGCGCAGCCTCAACCTCAACCTCAACGCCATCGTCGCCAGAAGAGGAGATAACGCTGACAGTGAAGTGGAGCGGAAAGGAGTACACCGTTCGAGTATGCGGCGACGACACGGTGGGCGAGCTGAAGCGCCGCATCTGCCAACTCACCACCGTCTTGCCCAAACGTCAAAAGCTTCTCTACCCAAAACTCCTCCCGTCCAAGCTCGCCGACGACACCGTTTTGCTCTCCTCTCTTCCCTTCAAGCCCTCCCAAAAGATGACCATGATCGGTACCATGGAGGATGATATTATAGTGGATCCGGTGGAGTCTCCGGAGATTGTTGACGATTTTGAGATTGCCCAAGATGAAGCTGTTGAGATTAAAGATAAGTATGCCAACAAGTTGAGGAGGCGTGTCGATCAATATAAGATTGAACTTCGAAATCCTTGCCGGGAAGGAAAGAAACTGCTTGTGCTGGATATTGATTATACTCTGTTTGATCACCGGTCCACAGCAGAGAACCCGCTTCAACTTATGCGGCCTTTTCTTCACGAGTTTCTTACAGCTGCTTATGCGGAATATGACATCATGATATGGTCTGCAACTAGCATGAAATGGGTTGAATTGAAGATGGGTCAGCTTGGAGTGCTGAGCAATCCCAACTACAAAATCACAGCTCTTCTAGACCATATGGCAATGATCACAGTTCAATGCGAGTCTCGTGGAACCTTTGATTGCAAGCCATTAGGCTTGATTTGGGCTAAATTCCCTGAGTTCTACAGTTCAAAAAACACCATAATGTTTGATGATCTGCGAAGAAACTTTGTGATGAACCCTCAAAATGGTTTGATCATCAAGCCATTCAAGAAGGCCCATTCTAGTCGAGACAGTGATCAGGAGCTCTTGAAGCTCACAACATACTTGCTTGCCATTGCAGAACTTGATGATTTGAGCAACCTTGATCACCATAACTGGCAGTCATTTTCTGAGGACAATGTCAAAAGACGTCGGCATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF ubiquitin

Protein Analysis

340

Amino Acids

38.76

Weight (kDa)

6.03

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 26 - 67 4.8e-08 Ubiquitin family
NIF PF03031 155 - 317 9.3e-24 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 5
AatII GACGTC 1 cut(s) 1009
Acc36I ACCTGC 1 cut(s) 5
Acc65I GGTACC 1 cut(s) 290
AccB1I GGYRCC 1 cut(s) 290
AccBSI CCGCTC 1 cut(s) 93
AccIII TCCGGA 1 cut(s) 331
AciI CCGC 6 cut(s) 93, 120, 151, 524, 538, 575
AclWI GGATC 2 cut(s) 311, 324
AcsI RAATTY 1 cut(s) 764
AcuI CTGAAG 1 cut(s) 164
AcyI GRCGYC 1 cut(s) 1006
AfaI GTAC 2 cut(s) 104, 292
AfiI CCNNNNNNNGG 1 cut(s) 842
AgeI ACCGGT 1 cut(s) 504
AgsI TTSAA 9 cut(s) 262, 434, 530, 623, 628, 710, 786, 865, 907
AluBI AGCT 9 cut(s) 142, 193, 223, 371, 566, 640, 677, 901, 910
AluI AGCT 9 cut(s) 142, 193, 223, 371, 566, 640, 677, 901, 910
Alw21I GWGCWC 1 cut(s) 903
Alw26I GTCTC 3 cut(s) 333, 723, 879
AlwI GGATC 2 cut(s) 311, 324
Aor13HI TCCGGA 1 cut(s) 331
AoxI GGCC 2 cut(s) 539, 870
ApeKI GCWGC 2 cut(s) 26, 566
ApoI RAATTY 1 cut(s) 764
AsiGI ACCGGT 1 cut(s) 504
Asp718I GGTACC 1 cut(s) 290
AspLEI GCGC 2 cut(s) 26, 150
AspS9I GGNCC 2 cut(s) 507, 871
AsuC2I CCSGG 1 cut(s) 452
AsuHPI GGTGA 3 cut(s) 157, 494, 956
AsuII TTCGAA 1 cut(s) 439
AvaII GGWCC 1 cut(s) 507
BamHI GGATCC 1 cut(s) 316
BanI GGYRCC 1 cut(s) 290
BanII GRGCYC 1 cut(s) 903
BauI CACGAG 2 cut(s) 551, 720
Bbv12I GWGCWC 1 cut(s) 903
BbvI GCAGC 2 cut(s) 38, 553
BccI CCATC 2 cut(s) 58, 625
BcgI CGANNNNNNTGC 2 cut(s) 429, 463
BclI TGATCA 5 cut(s) 499, 699, 849, 892, 961
BcnI CCSGG 1 cut(s) 452
BcoDI GTCTC 3 cut(s) 333, 723, 879
BfaI CTAG 3 cut(s) 606, 683, 879
BfmI CTRYAG 1 cut(s) 778
BfoI RGCGCY 1 cut(s) 151
BfuAI ACCTGC 1 cut(s) 5
BisI GCNGC 5 cut(s) 27, 121, 151, 539, 567
BlpI GCTNAGC 1 cut(s) 650
BlsI GCNGC 5 cut(s) 28, 122, 152, 540, 568
Bme1390I CCNGG 1 cut(s) 452
Bme18I GGWCC 1 cut(s) 507
BmgT120I GGNCC 2 cut(s) 507, 871
BmiI GGNNCC 3 cut(s) 292, 318, 727
BmrFI CCNGG 1 cut(s) 452
BmsI GCATC 1 cut(s) 162
Bpu1102I GCTNAGC 1 cut(s) 650
Bpu14I TTCGAA 1 cut(s) 439
BpuMI CCSGG 1 cut(s) 452
BsaHI GRCGYC 1 cut(s) 1006
BsaJI CCNNGG 1 cut(s) 294
BsaWI WCCGGW 3 cut(s) 319, 331, 504
Bsc4I CCNNNNNNNGG 1 cut(s) 842
Bse118I RCCGGY 1 cut(s) 504
Bse1I ACTGG 1 cut(s) 977
Bse3DI GCAATG 2 cut(s) 702, 930
BseAI TCCGGA 1 cut(s) 331
BseDI CCNNGG 1 cut(s) 294
BseGI GGATG 1 cut(s) 307
BseLI CCNNNNNNNGG 1 cut(s) 842
BseMI GCAATG 2 cut(s) 702, 930
BseMII CTCAG 3 cut(s) 641, 762, 978
BseNI ACTGG 1 cut(s) 977
BseRI GAGGAG 4 cut(s) 80, 200, 238, 421
BseXI GCAGC 2 cut(s) 38, 553
BsgI GTGCAG 1 cut(s) 39
BshFI GGCC 2 cut(s) 541, 872
BshNI GGYRCC 1 cut(s) 290
BshTI ACCGGT 1 cut(s) 504
BsiHKAI GWGCWC 1 cut(s) 903
BsiSI CCGG 4 cut(s) 320, 332, 451, 505
BslI CCNNNNNNNGG 1 cut(s) 842
BsmAI GTCTC 3 cut(s) 333, 723, 879
BsnI GGCC 2 cut(s) 541, 872
Bsp119I TTCGAA 1 cut(s) 439
Bsp1286I GDGCHC 1 cut(s) 903
Bsp13I TCCGGA 1 cut(s) 331
Bsp143I GATC 9 cut(s) 285, 316, 418, 499, 699, 808, 849, 892, 961
Bsp1720I GCTNAGC 1 cut(s) 650
Bsp19I CCATGG 1 cut(s) 294
BspACI CCGC 6 cut(s) 93, 120, 151, 524, 538, 575
BspANI GGCC 2 cut(s) 541, 872
BspCNI CTCAG 3 cut(s) 642, 763, 979
BspEI TCCGGA 1 cut(s) 331
BspHI TCATGA 1 cut(s) 588
BspLI GGNNCC 3 cut(s) 292, 318, 727
BspMI ACCTGC 1 cut(s) 5
BspPI GGATC 2 cut(s) 311, 324
BspQI GCTCTTC 1 cut(s) 684
BspT104I TTCGAA 1 cut(s) 439
BspT107I GGYRCC 1 cut(s) 290
BsrBI CCGCTC 1 cut(s) 93
BsrDI GCAATG 2 cut(s) 702, 930
BsrFI RCCGGY 1 cut(s) 504
BsrI ACTGG 1 cut(s) 977
BssAI RCCGGY 1 cut(s) 504
BssECI CCNNGG 1 cut(s) 294
BssMI GATC 9 cut(s) 285, 316, 418, 499, 699, 808, 849, 892, 961
BssNI GRCGYC 1 cut(s) 1006
BssSI CACGAG 2 cut(s) 551, 720
BssT1I CCWWGG 1 cut(s) 294
Bst2BI CACGAG 2 cut(s) 551, 720
Bst4CI ACNGT 8 cut(s) 82, 109, 133, 172, 238, 706, 782, 890
Bst6I CTCTTC 3 cut(s) 57, 258, 684
BstACI GRCGYC 1 cut(s) 1006
BstBI TTCGAA 1 cut(s) 439
BstC8I GCNNGC 5 cut(s) 22, 140, 225, 740, 927
BstDEI CTNAG 3 cut(s) 650, 771, 987
BstDSI CCRYGG 1 cut(s) 294
BstF5I GGATG 1 cut(s) 307
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 2 cut(s) 26, 150
BstKTI GATC 9 cut(s) 288, 319, 421, 502, 702, 811, 852, 895, 964
BstMAI GTCTC 3 cut(s) 333, 723, 879
BstMBI GATC 9 cut(s) 285, 316, 418, 499, 699, 808, 849, 892, 961
BstMWI GCNNNNNNNGC 7 cut(s) 11, 26, 55, 156, 572, 646, 907
BstSCI CCNGG 1 cut(s) 450
BstSFI CTRYAG 1 cut(s) 778
BstV1I GCAGC 2 cut(s) 38, 553
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
BsuRI GGCC 2 cut(s) 541, 872
BtgI CCRYGG 1 cut(s) 294
BtsCI GGATG 1 cut(s) 307
BtsIMutI CAGTG 2 cut(s) 87, 895
BveI ACCTGC 1 cut(s) 5
Cac8I GCNNGC 5 cut(s) 22, 140, 225, 740, 927
CciI TCATGA 1 cut(s) 588
CfoI GCGC 2 cut(s) 26, 150
Cfr10I RCCGGY 1 cut(s) 504
Cfr13I GGNCC 2 cut(s) 507, 871
Csp6I GTAC 2 cut(s) 103, 291
CspAI ACCGGT 1 cut(s) 504
CviAII CATG 4 cut(s) 283, 295, 589, 610
CviQI GTAC 2 cut(s) 103, 291
DdeI CTNAG 3 cut(s) 650, 771, 987
DpnI GATC 9 cut(s) 287, 318, 420, 501, 701, 810, 851, 894, 963
DpnII GATC 9 cut(s) 285, 316, 418, 499, 699, 808, 849, 892, 961
Eam1104I CTCTTC 3 cut(s) 57, 258, 684
EarI CTCTTC 3 cut(s) 57, 258, 684
Ecl136II GAGCTC 1 cut(s) 901
Eco130I CCWWGG 1 cut(s) 294
Eco24I GRGCYC 1 cut(s) 903
Eco47I GGWCC 1 cut(s) 507
Eco53kI GAGCTC 1 cut(s) 901
Eco57I CTGAAG 1 cut(s) 164
EcoICRI GAGCTC 1 cut(s) 901
EcoT14I CCWWGG 1 cut(s) 294
EcoT38I GRGCYC 1 cut(s) 903
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 4 cut(s) 286, 298, 592, 613
FatI CATG 4 cut(s) 282, 294, 588, 609
FauI CCCGC 1 cut(s) 531
FauNDI CATATG 1 cut(s) 690
FbaI TGATCA 5 cut(s) 499, 699, 849, 892, 961
Fnu4HI GCNGC 5 cut(s) 27, 121, 151, 539, 567
FokI GGATG 1 cut(s) 314
FriOI GRGCYC 1 cut(s) 903
Fsp4HI GCNGC 5 cut(s) 27, 121, 151, 539, 567
FspBI CTAG 3 cut(s) 606, 683, 879
GlaI GCGC 2 cut(s) 25, 149
GluI GCNGC 5 cut(s) 27, 121, 151, 539, 567
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 2 cut(s) 541, 872
HapII CCGG 4 cut(s) 320, 332, 451, 505
HhaI GCGC 2 cut(s) 26, 150
Hin1I GRCGYC 1 cut(s) 1006
Hin1II CATG 4 cut(s) 286, 298, 592, 613
Hin6I GCGC 2 cut(s) 24, 148
HinP1I GCGC 2 cut(s) 24, 148
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 2 cut(s) 326, 716
HpaII CCGG 4 cut(s) 320, 332, 451, 505
HphI GGTGA 3 cut(s) 157, 494, 956
Hpy166II GTNNAC 3 cut(s) 105, 343, 510
Hpy188I TCNGA 1 cut(s) 988
Hpy188III TCNNGA 8 cut(s) 332, 551, 589, 683, 865, 884, 896, 904
Hpy8I GTNNAC 3 cut(s) 105, 343, 510
Hpy99I CGWCG 4 cut(s) 58, 128, 233, 1011
HpyAV CCTTC 3 cut(s) 268, 449, 862
HpyCH4III ACNGT 8 cut(s) 82, 109, 133, 172, 238, 706, 782, 890
HpyCH4IV ACGT 2 cut(s) 184, 1006
HpyCH4V TGCA 4 cut(s) 20, 602, 738, 935
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 26, 55, 156, 572, 646, 907
HpyF3I CTNAG 3 cut(s) 650, 771, 987
HpySE526I ACGT 2 cut(s) 184, 1006
Hsp92I GRCGYC 1 cut(s) 1006
Hsp92II CATG 4 cut(s) 286, 298, 592, 613
HspAI GCGC 2 cut(s) 24, 148
Kpn2I TCCGGA 1 cut(s) 331
KpnI GGTACC 1 cut(s) 294
Ksp22I TGATCA 5 cut(s) 499, 699, 849, 892, 961
Kzo9I GATC 9 cut(s) 285, 316, 418, 499, 699, 808, 849, 892, 961
LguI GCTCTTC 1 cut(s) 684
LmnI GCTCC 2 cut(s) 90, 898
Lsp1109I GCAGC 2 cut(s) 38, 553
LweI GCATC 1 cut(s) 162
MaeI CTAG 3 cut(s) 606, 683, 879
MaeII ACGT 2 cut(s) 184, 1006
MalI GATC 9 cut(s) 287, 318, 420, 501, 701, 810, 851, 894, 963
MbiI CCGCTC 1 cut(s) 93
MboI GATC 9 cut(s) 285, 316, 418, 499, 699, 808, 849, 892, 961
MboII GAAGA 6 cut(s) 74, 245, 539, 640, 671, 828
MflI RGATCY 1 cut(s) 316
MhlI GDGCHC 1 cut(s) 903
MluCI AATT 2 cut(s) 623, 764
MlyI GAGTC 2 cut(s) 335, 725
MroI TCCGGA 1 cut(s) 331
MseI TTAA 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 797
MspA1I CMGCKG 1 cut(s) 566
MspI CCGG 4 cut(s) 320, 332, 451, 505
MspR9I CCNGG 1 cut(s) 452
MwoI GCNNNNNNNGC 7 cut(s) 11, 26, 55, 156, 572, 646, 907
NciI CCSGG 1 cut(s) 452
NcoI CCATGG 1 cut(s) 294
NdeI CATATG 1 cut(s) 690
NdeII GATC 9 cut(s) 285, 316, 418, 499, 699, 808, 849, 892, 961
NlaIII CATG 4 cut(s) 286, 298, 592, 613
NlaIV GGNNCC 3 cut(s) 292, 318, 727
NspV TTCGAA 1 cut(s) 439
PagI TCATGA 1 cut(s) 588
PaqCI CACCTGC 1 cut(s) 5
PciSI GCTCTTC 1 cut(s) 684
PflFI GACNNNGTC 1 cut(s) 995
PinAI ACCGGT 1 cut(s) 504
PkrI GCNGC 5 cut(s) 28, 122, 152, 540, 568
PleI GAGTC 2 cut(s) 334, 724
PpsI GAGTC 2 cut(s) 334, 724
Psp124BI GAGCTC 1 cut(s) 903
PspN4I GGNNCC 3 cut(s) 292, 318, 727
PspPI GGNCC 2 cut(s) 507, 871
PsuI RGATCY 1 cut(s) 316
PsyI GACNNNGTC 1 cut(s) 995
PvuII CAGCTG 1 cut(s) 566
RsaI GTAC 2 cut(s) 104, 292
RsaNI GTAC 2 cut(s) 103, 291
RseI CAYNNNNRTG 1 cut(s) 797
SacI GAGCTC 1 cut(s) 903
SapI GCTCTTC 1 cut(s) 684
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 5 cut(s) 27, 121, 151, 539, 567
Sau3AI GATC 9 cut(s) 285, 316, 418, 499, 699, 808, 849, 892, 961
Sau96I GGNCC 2 cut(s) 507, 871
SchI GAGTC 2 cut(s) 335, 725
ScrFI CCNGG 1 cut(s) 452
SduI GDGCHC 1 cut(s) 903
SfaNI GCATC 1 cut(s) 162
SfcI CTRYAG 1 cut(s) 778
SfuI TTCGAA 1 cut(s) 439
SinI GGWCC 1 cut(s) 507
SmiMI CAYNNNNRTG 1 cut(s) 797
Sse9I AATT 2 cut(s) 623, 764
SsiI CCGC 6 cut(s) 93, 120, 151, 524, 538, 575
SspMI CTAG 3 cut(s) 606, 683, 879
SstI GAGCTC 1 cut(s) 903
StyD4I CCNGG 1 cut(s) 450
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 8 cut(s) 82, 109, 133, 172, 238, 706, 782, 890
TaiI ACGT 2 cut(s) 187, 1009
TaqI TCGA 4 cut(s) 112, 417, 439, 883
TasI AATT 2 cut(s) 623, 764
TatI WGTACW 1 cut(s) 102
TauI GCSGC 3 cut(s) 123, 153, 541
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 2 cut(s) 87, 895
TseI GCWGC 2 cut(s) 26, 566
TspDTI ATGAA 3 cut(s) 381, 626, 845
TspRI CASTG 2 cut(s) 87, 895
Tth111I GACNNNGTC 1 cut(s) 995
VpaK11BI GGWCC 1 cut(s) 507
XapI RAATTY 1 cut(s) 764
XbaI TCTAGA 1 cut(s) 682
XspI CTAG 3 cut(s) 606, 683, 879
ZraI GACGTC 1 cut(s) 1007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.