Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002956

Physical Location & Seq

Genomic Coordinates Reverse (-)

6040 .. 14598

Gene Structure 8559 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1000200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2523 bp

ATGAATATCCCACCTGCTGTTGCACAAGAAACTGGTAATGCCTCATTGTCCTCAACTAGCTCTCATTCTGGTTCCTTGCCTGCTCCTACCACTTCAAATTCCACAATGAATATATCATCAACTCCTAATGCGGGACCAAAAACTTTATGGGTTTCCACTGCCCCATCTTTTAACATGACTCCTGGAATGCCTGGAACTCCCAGGACACCTGGACCCCCTGGAATAGCTCACTCAGTGCAAATATCTTTTAATCCGACTGTACCATCTGCACCTATTGATTCTTCTGTAGCCAATAGACCTAGCATGCAAGCAGTACCTGTTGCATCATCAGCAGTTCAGCCACATGTGAGTGCTCCTTACCCATCTTTATCTGCTATGGGTGCTCCCTGGCTCTCGTCCCCACAGATAGGAGGCTTACCTAGGCCTCCGTTTTTGCCATATCCTGCCGCCTTTCCTGGTCCCTTTCCTCTGCCTGCACATGTTATGCCTCTCGCTTCTGTTCCTTTACCTGATTCTCAGCCTCCTGGTGTCACTCCTGTGGGAAATACTGCAGCAAATGCCGTGTCTTCCGTTGGTTCTGGTCATCAATTGGCAGGGTCTTCAGTGATGCAGAAAGAATTACCTCATCCTGGCGTTGGTCCGGAGAATAGAGCTGCTGTCAATGAACAATTGGTTGCCTGGACTGCCCACAAGACAGAGACAGGAGTTGTATACTATTATAATGCTTTGACCGGTGAATCAACTTATGATAAGCCTCCTGGATTTAAGGAGGAGCCTGATAAGGTTTCTATGCAGCCAACTCCAGTTTCGACGGTGAATTTGGCTGGAACTGATTGGGTATTGGTCACAACCAGTGATGGGAAAAAGTTTTACCATAATAGCAAAACCAAGGTAAGTAGCTGGCAGATCCCAAATGAAGTGATTGAATTGAAAAAGCAGCAGGATAGTGACGTGCCAAAAGAACATACACTATCAGTGCCAAATAATAATTTAATGATTGAAAAAGGATCTGCTCCAGTTAGTATGAGTGCTCCGGCCATTAACACGGGTGGTCGTGAAGCCATGCCTTTTAAACCTTCAGCTGTGCTGGGAACATCTTCTGCACTTGATCTGATTAAAAGGAAGTTGCAGGATCCTGTTACTTCTTCACCAATTCCGGCACCATCAGAATCAAATGGTGCAAGAGGAGTTGAGAGTACACCTAAGGGCCAGCAAAGCGAGAATAGCAAAGATAAGCTAAAAGAAACTAATGGAGATGGAAACTTGTCTGATTCCTCATCAGATTCAGAGGATGCAGACAGTGGGCCAACAAAGGAGGAGTGTATTATTCAATTCAAGATGCTTAAAGAACGGGGAGTGGCACCATTCTCGAAGTGGGAGAAGGAACTGCCAAAGATAGTGTTTGATCCACGATTTAAGGCCATTCCAAGTCATGAAGCTCGTAGGTCCTTGTTTGAGCACTATGTTAAAACACGTGCAGAGGAGGAGAGAAAGGAAAAAAGAGCAGCCCAGAAGGCTGCAATAGAGGGATTTAAGCAGTTATTGGATGAAGCATCTGAGGATATTGATCGAAACACTGATTACCAGAGTTTCAGAAGGAAATGGGGCAATGATCCACGCTTTGAGGCTTTAGATCGCAAAGATCGGGAGCATTTGTTGAATGAAAGGGTCCTTCCATTGAAGAGGGCTGCTGAAGAAAAGGTTCAAGCAGTGCGTGCTGCTGCTTCAGCTGGTTTTAAGTCCATGCTTAAAGAAAAAGGAGATATTACTGTTAGTTCTCGTTGGTCCAGGGTGAAAGATAATCTACGGAATGACCCAAGGTACAAAAATGTTAGGCATGAGGATAGGGAAGCTTTATTCAATGAGTATATATCGGGACTAAAAGCTGTGGAAGAGGAAGCAGAACGAGAGGCAAAAGCTAAACGTGATGAGCAGGAAAAACTGAGGGAAAGGGAACGAGAACTGCGGAAGCGGAAGGAAAGAGAAGAGCAAGAAACAGAGAGGGTGCGACTGAAAGTTCGCAGGAAGGAGGCAGTTGCTACTTTCCAAGCTTTACTTGTGGAAACAATTAAAGATCCTCAGGCATCTTGGACAGGCTCTAGGCCTAAATTGGAGAAGGATCCGCAAAGGCGTGCTGCCAACCCTGATTTGGATCCATCTGACATGGAAAAGCTCTTCCGGGAACATGTAAAGATGCTAAACGAGCGATGTGCACATGAGTTCAGAACTCTGTTGGCCGAAGTCTTGACTGCAGAAGCAGCGTCCCAGGAGACAGAAGATGGAAAAACAGTTCTTAATTCGTGGTCAACAGCCAAACGCATTCTAAAGGTGGATCCCAGATACGACAAGACGCCAAGAAAAGAGAGGGAAGTGTTATGGAGACGATATTCTGAGGAGATGCTGCGGAAGCAAAAGTCAGCAGTTGATCAGAAGGAAGACAGGAAAACAGATGCTAAAACTAGAAGTTCTGCTGATGCTGGGAGGAATCCCTATGGATCTAGGGGTACTCACGATCGGCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0001085 GO:0001103 GO:0003674 GO:0003712 GO:0003713 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045892 GO:0045934 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0065007 GO:0070013 GO:0070491 GO:0071704 GO:0080090 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03040 ko04212 map03040 map04212

Pfam Domains

Protein Families

FF WW

Protein Analysis

841

Amino Acids

92.86

Weight (kDa)

9.1

Isoelectric Point (pI)

51.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WW	PF00397	227 - 252	1.1e-07	WW domain
WW_TCERG1	PF23517	266 - 309	1.4e-09	TCERG1 WW domain
WW	PF00397	277 - 304	1.4e-06	WW domain
FF	PF01846	445 - 488	1.1e-11	FF domain
FF	PF01846	507 - 555	9.1e-09	FF domain
FF	PF01846	575 - 623	1.3e-13	FF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002956)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G19840
fragaria_vesca	FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700
malus_domestica	MD13G1007900.v1.1 MD13G1008000.v1.1 MD16G1000200.v1.1
prunus_persica	Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1
pyrus_communis	pycom13g00650 pycom16g00500 pycom16g00510 pycom16g00520
rosa_chinensis	RchiOBHm_Chr4g0446421
rosa_laevigata	RLG00000005662
rosa_multiflora	Rmu_co8175444.1_g000001 Rmu_sc0008360.1_g000005 Rmu_sc0018208.1_g000001 Rmu_sc0025430.1_g000001
rosa_roxburghii	Rroxscaffold_5G00386860
rosa_rugosa	Rorug04G0371200
rosa_samantha	Rh4AG432800 Rh4BG441900 Rh4CG459300 Rh4DG440700
rosa_wichuraiana	Rw4G036970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	720
AarI	CACCTGC	1 cut(s)	22
Acc36I	ACCTGC	1 cut(s)	22
AccB1I	GGYRCC	2 cut(s)	1159, 1360
AccI	GTMKAC	1 cut(s)	711
AccIII	TCCGGA	1 cut(s)	640
AciI	CCGC	6 cut(s)	131, 447, 1966, 1972, 2123, 2404
AcoI	YGGCCR	2 cut(s)	1035, 2235
AcsI	RAATTY	2 cut(s)	97, 817
AcuI	CTGAAG	4 cut(s)	585, 1062, 1710, 1713
AcvI	CACGTG	1 cut(s)	1475
AcyI	GRCGYC	1 cut(s)	2351
AdeI	CACNNNGTG	1 cut(s)	235
AfaI	GTAC	5 cut(s)	261, 315, 1198, 1823, 2506
AfiI	CCNNNNNNNGG	6 cut(s)	131, 407, 629, 635, 858, 2149
AflIII	ACRYGT	4 cut(s)	343, 478, 1472, 2185
AgeI	ACCGGT	1 cut(s)	731
AjiI	CACGTC	1 cut(s)	952
AjuI	GAANNNNNNNTTGG	2 cut(s)	790, 822
AleI	CACNNNNGTG	1 cut(s)	536
AloI	GAACNNNNNNTCC	2 cut(s)	2274, 2306
Alw21I	GWGCWC	5 cut(s)	355, 385, 1033, 1461, 2215
Alw26I	GTCTC	3 cut(s)	692, 2264, 2374
Alw44I	GTGCAC	1 cut(s)	2211
AlwNI	CAGNNNCTG	1 cut(s)	317
Aor13HI	TCCGGA	1 cut(s)	640
AoxI	GGCC	7 cut(s)	422, 1035, 1207, 1304, 1419, 2102, 2235
ApaLI	GTGCAC	1 cut(s)	2211
ApoI	RAATTY	2 cut(s)	97, 817
ArsI	GACNNNNNNTTYG	6 cut(s)	548, 580, 802, 834, 2001, 2033
AsiGI	ACCGGT	1 cut(s)	731
Asp700I	GAANNNNTTC	2 cut(s)	1096, 1701
AspA2I	CCTAGG	1 cut(s)	419
AspS9I	GGNCC	9 cut(s)	134, 212, 458, 638, 1207, 1304, 1446, 1669, 1785
AsuC2I	CCSGG	1 cut(s)	2180
AsuHPI	GGTGA	4 cut(s)	746, 826, 1140, 1804
AvaII	GGWCC	7 cut(s)	134, 212, 458, 638, 1446, 1669, 1785
AvrII	CCTAGG	1 cut(s)	419
AxyI	CCTNAGG	2 cut(s)	1203, 2079
BaeGI	GKGCMC	1 cut(s)	2215
BamHI	GGATCC	4 cut(s)	1132, 2119, 2152, 2332
BanI	GGYRCC	2 cut(s)	1159, 1360
BbrPI	CACGTG	1 cut(s)	1475
BbsI	GAAGAC	3 cut(s)	558, 591, 2442
Bbv12I	GWGCWC	5 cut(s)	355, 385, 1033, 1461, 2215
BccI	CCATC	8 cut(s)	172, 271, 370, 851, 1171, 1250, 2164, 2273
BceAI	ACGGC	1 cut(s)	545
BcgI	CGANNNNNNTGC	4 cut(s)	1350, 1384, 2192, 2226
BclI	TGATCA	1 cut(s)	2425
BcnI	CCSGG	1 cut(s)	2180
BcoDI	GTCTC	3 cut(s)	692, 2264, 2374
BfaI	CTAG	6 cut(s)	57, 300, 420, 2100, 2460, 2499
BfmI	CTRYAG	3 cut(s)	285, 549, 2250
BfuAI	ACCTGC	1 cut(s)	22
BglI	GCCNNNNNGGC	1 cut(s)	1514
BlnI	CCTAGG	1 cut(s)	419
Bme18I	GGWCC	7 cut(s)	134, 212, 458, 638, 1446, 1669, 1785
BmgBI	CACGTC	1 cut(s)	952
BmgT120I	GGNCC	9 cut(s)	134, 212, 458, 638, 1207, 1304, 1446, 1669, 1785
BpiI	GAAGAC	3 cut(s)	558, 591, 2442
BpmI	CTGGAG	2 cut(s)	786, 999
BpuMI	CCSGG	1 cut(s)	2180
BsaAI	YACGTR	1 cut(s)	1475
BsaBI	GATNNNNATC	2 cut(s)	1566, 2151
BsaHI	GRCGYC	1 cut(s)	2351
BsaJI	CCNNGG	8 cut(s)	200, 217, 386, 419, 888, 1788, 1817, 2265
BsaWI	WCCGGW	2 cut(s)	640, 731
BsaXI	ACNNNNNCTCC	2 cut(s)	1640, 1670
Bsc4I	CCNNNNNNNGG	6 cut(s)	131, 407, 629, 635, 858, 2149
Bse118I	RCCGGY	1 cut(s)	731
Bse1I	ACTGG	4 cut(s)	37, 803, 852, 1016
Bse21I	CCTNAGG	2 cut(s)	1203, 2079
Bse3DI	GCAATG	1 cut(s)	1615
Bse8I	GATNNNNATC	2 cut(s)	1566, 2151
BseAI	TCCGGA	1 cut(s)	640
BseDI	CCNNGG	8 cut(s)	200, 217, 386, 419, 888, 1788, 1817, 2265
BseGI	GGATG	3 cut(s)	625, 1297, 1552
BseJI	GATNNNNATC	2 cut(s)	1566, 2151
BseLI	CCNNNNNNNGG	6 cut(s)	131, 407, 629, 635, 858, 2149
BseMI	GCAATG	1 cut(s)	1615
BseMII	CTCAG	6 cut(s)	246, 530, 1548, 1934, 2093, 2382
BseNI	ACTGG	4 cut(s)	37, 803, 852, 1016
BseRI	GAGGAG	6 cut(s)	785, 1200, 1331, 1496, 1499, 2408
BseSI	GKGCMC	1 cut(s)	2215
BseYI	CCCAGC	2 cut(s)	1087, 2477
BsgI	GTGCAG	4 cut(s)	252, 459, 1086, 1497
Bsh1285I	CGRYCG	1 cut(s)	2515
BshFI	GGCC	7 cut(s)	424, 1037, 1209, 1306, 1421, 2104, 2237
BshNI	GGYRCC	2 cut(s)	1159, 1360
BshTI	ACCGGT	1 cut(s)	731
BsiEI	CGRYCG	1 cut(s)	2515
BsiHKAI	GWGCWC	5 cut(s)	355, 385, 1033, 1461, 2215
BsiSI	CCGG	5 cut(s)	641, 732, 1034, 1157, 2179
BslFI	GGGAC	5 cut(s)	147, 382, 444, 1890, 2248
BslI	CCNNNNNNNGG	6 cut(s)	131, 407, 629, 635, 858, 2149
BsmAI	GTCTC	3 cut(s)	692, 2264, 2374
BsmBI	CGTCTC	1 cut(s)	2374
BsmFI	GGGAC	5 cut(s)	147, 382, 444, 1890, 2248
BsmI	GAATGC	2 cut(s)	192, 2319
BsnI	GGCC	7 cut(s)	424, 1037, 1209, 1306, 1421, 2104, 2237
Bsp1286I	GDGCHC	5 cut(s)	355, 385, 1033, 1461, 2215
Bsp13I	TCCGGA	1 cut(s)	640
BspACI	CCGC	6 cut(s)	131, 447, 1966, 1972, 2123, 2404
BspANI	GGCC	7 cut(s)	424, 1037, 1209, 1306, 1421, 2104, 2237
BspCNI	CTCAG	6 cut(s)	245, 529, 1549, 1935, 2092, 2383
BspEI	TCCGGA	1 cut(s)	640
BspHI	TCATGA	1 cut(s)	1432
BspMAI	CTGCAG	2 cut(s)	553, 2254
BspMI	ACCTGC	1 cut(s)	22
BspQI	GCTCTTC	2 cut(s)	1980, 2180
BspT107I	GGYRCC	2 cut(s)	1159, 1360
BsrDI	GCAATG	1 cut(s)	1615
BsrFI	RCCGGY	1 cut(s)	731
BsrI	ACTGG	4 cut(s)	37, 803, 852, 1016
BssAI	RCCGGY	1 cut(s)	731
BssECI	CCNNGG	8 cut(s)	200, 217, 386, 419, 888, 1788, 1817, 2265
BssNAI	GTATAC	1 cut(s)	712
BssNI	GRCGYC	1 cut(s)	2351
BssT1I	CCWWGG	3 cut(s)	419, 888, 1817
Bst1107I	GTATAC	1 cut(s)	712
Bst4CI	ACNGT	5 cut(s)	259, 814, 1301, 1771, 2290
Bst6I	CTCTTC	4 cut(s)	1676, 1887, 1980, 2180
BstACI	GRCGYC	1 cut(s)	2351
BstAPI	GCANNNNNTGC	2 cut(s)	557, 1715
BstBAI	YACGTR	1 cut(s)	1475
BstC8I	GCNNGC	8 cut(s)	81, 305, 309, 474, 902, 1211, 1716, 2133
BstDEI	CTNAG	7 cut(s)	232, 516, 1203, 1557, 1943, 2079, 2391
BstF5I	GGATG	3 cut(s)	625, 1297, 1552
BstMAI	GTCTC	3 cut(s)	692, 2264, 2374
BstMCI	CGRYCG	1 cut(s)	2515
BstNSI	RCATGY	4 cut(s)	307, 347, 482, 2189
BstSFI	CTRYAG	3 cut(s)	285, 549, 2250
BstSLI	GKGCMC	1 cut(s)	2215
BstV2I	GAAGAC	3 cut(s)	558, 591, 2442
BstX2I	RGATCY	8 cut(s)	906, 1007, 1132, 2074, 2119, 2152, 2332, 2495
BstYI	RGATCY	8 cut(s)	906, 1007, 1132, 2074, 2119, 2152, 2332, 2495
BstZ17I	GTATAC	1 cut(s)	712
Bsu36I	CCTNAGG	2 cut(s)	1203, 2079
BsuRI	GGCC	7 cut(s)	424, 1037, 1209, 1306, 1421, 2104, 2237
BtgZI	GCGATG	1 cut(s)	2221
BtrI	CACGTC	1 cut(s)	952
BtsCI	GGATG	3 cut(s)	625, 1297, 1552
BtsI	GCAGTG	2 cut(s)	156, 1716
BtsIMutI	CAGTG	8 cut(s)	156, 240, 609, 859, 981, 1306, 1575, 1716
BveI	ACCTGC	1 cut(s)	22
Cac8I	GCNNGC	8 cut(s)	81, 305, 309, 474, 902, 1211, 1716, 2133
CaiI	CAGNNNCTG	1 cut(s)	317
CciI	TCATGA	1 cut(s)	1432
Cfr10I	RCCGGY	1 cut(s)	731
Cfr13I	GGNCC	9 cut(s)	134, 212, 458, 638, 1207, 1304, 1446, 1669, 1785
CseI	GACGC	2 cut(s)	2250, 2359
Csp6I	GTAC	5 cut(s)	260, 314, 1197, 1822, 2505
CspAI	ACCGGT	1 cut(s)	731
CviQI	GTAC	5 cut(s)	260, 314, 1197, 1822, 2505
DdeI	CTNAG	7 cut(s)	232, 516, 1203, 1557, 1943, 2079, 2391
DraI	TTTAAA	1 cut(s)	1072
DraIII	CACNNNGTG	1 cut(s)	235
EaeI	YGGCCR	2 cut(s)	1035, 2235
Eam1104I	CTCTTC	4 cut(s)	1676, 1887, 1980, 2180
EarI	CTCTTC	4 cut(s)	1676, 1887, 1980, 2180
Eco130I	CCWWGG	3 cut(s)	419, 888, 1817
Eco147I	AGGCCT	2 cut(s)	424, 2104
Eco47I	GGWCC	7 cut(s)	134, 212, 458, 638, 1446, 1669, 1785
Eco57I	CTGAAG	4 cut(s)	585, 1062, 1710, 1713
Eco72I	CACGTG	1 cut(s)	1475
Eco81I	CCTNAGG	2 cut(s)	1203, 2079
EcoO109I	RGGNCCY	2 cut(s)	1446, 1669
EcoT14I	CCWWGG	3 cut(s)	419, 888, 1817
ErhI	CCWWGG	3 cut(s)	419, 888, 1817
Esp3I	CGTCTC	1 cut(s)	2374
FalI	AAGNNNNNCTT	2 cut(s)	2040, 2072
FaqI	GGGAC	5 cut(s)	147, 382, 444, 1890, 2248
FauI	CCCGC	1 cut(s)	124
FbaI	TGATCA	1 cut(s)	2425
FblI	GTMKAC	1 cut(s)	711
FokI	GGATG	3 cut(s)	612, 1304, 1559
FspBI	CTAG	6 cut(s)	57, 300, 420, 2100, 2460, 2499
GsaI	CCCAGC	2 cut(s)	1091, 2481
GsuI	CTGGAG	2 cut(s)	786, 999
HaeIII	GGCC	7 cut(s)	424, 1037, 1209, 1306, 1421, 2104, 2237
HapII	CCGG	5 cut(s)	641, 732, 1034, 1157, 2179
HgaI	GACGC	2 cut(s)	2250, 2359
Hin1I	GRCGYC	1 cut(s)	2351
HincII	GTYRAC	1 cut(s)	2307
HindII	GTYRAC	1 cut(s)	2307
HindIII	AAGCTT	2 cut(s)	1851, 2049
HinfI	GANTC	8 cut(s)	178, 278, 512, 737, 1169, 1271, 1283, 2485
HpaII	CCGG	5 cut(s)	641, 732, 1034, 1157, 2179
HphI	GGTGA	4 cut(s)	746, 826, 1140, 1804
Hpy166II	GTNNAC	4 cut(s)	712, 1199, 2213, 2307
Hpy188III	TCNNGA	9 cut(s)	641, 1055, 1336, 1369, 1433, 1646, 1875, 2245, 2510
Hpy8I	GTNNAC	4 cut(s)	712, 1199, 2213, 2307
Hpy99I	CGWCG	1 cut(s)	814
HpyAV	CCTTC	9 cut(s)	1086, 1375, 1507, 1590, 1682, 1969, 2020, 2110, 2425
HpyCH4III	ACNGT	5 cut(s)	259, 814, 1301, 1771, 2290
HpyCH4IV	ACGT	3 cut(s)	951, 1474, 1924
HpyF3I	CTNAG	7 cut(s)	232, 516, 1203, 1557, 1943, 2079, 2391
HpySE526I	ACGT	3 cut(s)	951, 1474, 1924
Hsp92I	GRCGYC	1 cut(s)	2351
Kpn2I	TCCGGA	1 cut(s)	640
Ksp22I	TGATCA	1 cut(s)	2425
LguI	GCTCTTC	2 cut(s)	1980, 2180
LmnI	GCTCC	7 cut(s)	88, 358, 388, 772, 1018, 1036, 1648
MaeI	CTAG	6 cut(s)	57, 300, 420, 2100, 2460, 2499
MaeII	ACGT	3 cut(s)	951, 1474, 1924
MaeIII	GTNAC	4 cut(s)	529, 844, 947, 1138
MfeI	CAATTG	2 cut(s)	587, 668
MflI	RGATCY	8 cut(s)	906, 1007, 1132, 2074, 2119, 2152, 2332, 2495
MhlI	GDGCHC	5 cut(s)	355, 385, 1033, 1461, 2215
MlyI	GAGTC	1 cut(s)	172
MmeI	TCCRAC	1 cut(s)	278
MroI	TCCGGA	1 cut(s)	640
MroXI	GAANNNNTTC	2 cut(s)	1096, 1701
MslI	CAYNNNNRTG	2 cut(s)	348, 536
MspA1I	CMGCKG	2 cut(s)	1082, 1730
MspI	CCGG	5 cut(s)	641, 732, 1034, 1157, 2179
MunI	CAATTG	2 cut(s)	587, 668
Mva1269I	GAATGC	2 cut(s)	192, 2319
NciI	CCSGG	1 cut(s)	2180
NmuCI	GTSAC	3 cut(s)	529, 844, 947
NspI	RCATGY	4 cut(s)	307, 347, 482, 2189
OliI	CACNNNNGTG	1 cut(s)	536
PaeI	GCATGC	1 cut(s)	307
PagI	TCATGA	1 cut(s)	1432
PaqCI	CACCTGC	1 cut(s)	22
PceI	AGGCCT	2 cut(s)	424, 2104
PciI	ACATGT	3 cut(s)	343, 478, 2185
PciSI	GCTCTTC	2 cut(s)	1980, 2180
PctI	GAATGC	2 cut(s)	192, 2319
PdmI	GAANNNNTTC	2 cut(s)	1096, 1701
PfeI	GAWTC	7 cut(s)	278, 512, 737, 1169, 1271, 1283, 2485
PfoI	TCCNGGA	3 cut(s)	181, 757, 2178
PinAI	ACCGGT	1 cut(s)	731
Ple19I	CGATCG	1 cut(s)	2515
PleI	GAGTC	1 cut(s)	172
PmaCI	CACGTG	1 cut(s)	1475
PmlI	CACGTG	1 cut(s)	1475
PpsI	GAGTC	1 cut(s)	172
Ppu21I	YACGTR	1 cut(s)	1475
PpuMI	RGGWCCY	2 cut(s)	1446, 1669
PscI	ACATGT	3 cut(s)	343, 478, 2185
PsiI	TTATAA	1 cut(s)	720
Psp5II	RGGWCCY	2 cut(s)	1446, 1669
PspCI	CACGTG	1 cut(s)	1475
PspFI	CCCAGC	2 cut(s)	1087, 2477
PspPI	GGNCC	9 cut(s)	134, 212, 458, 638, 1207, 1304, 1446, 1669, 1785
PspPPI	RGGWCCY	2 cut(s)	1446, 1669
PstI	CTGCAG	2 cut(s)	553, 2254
PstNI	CAGNNNCTG	1 cut(s)	317
PsuI	RGATCY	8 cut(s)	906, 1007, 1132, 2074, 2119, 2152, 2332, 2495
PvuI	CGATCG	1 cut(s)	2515
PvuII	CAGCTG	2 cut(s)	1082, 1730
RsaI	GTAC	5 cut(s)	261, 315, 1198, 1823, 2506
RsaNI	GTAC	5 cut(s)	260, 314, 1197, 1822, 2505
RseI	CAYNNNNRTG	2 cut(s)	348, 536
SapI	GCTCTTC	2 cut(s)	1980, 2180
Sau96I	GGNCC	9 cut(s)	134, 212, 458, 638, 1207, 1304, 1446, 1669, 1785
SchI	GAGTC	1 cut(s)	172
SduI	GDGCHC	5 cut(s)	355, 385, 1033, 1461, 2215
SfcI	CTRYAG	3 cut(s)	285, 549, 2250
SinI	GGWCC	7 cut(s)	134, 212, 458, 638, 1446, 1669, 1785
SmiMI	CAYNNNNRTG	2 cut(s)	348, 536
SphI	GCATGC	1 cut(s)	307
SseBI	AGGCCT	2 cut(s)	424, 2104
SsiI	CCGC	6 cut(s)	131, 447, 1966, 1972, 2123, 2404
SspMI	CTAG	6 cut(s)	57, 300, 420, 2100, 2460, 2499
StuI	AGGCCT	2 cut(s)	424, 2104
StyI	CCWWGG	3 cut(s)	419, 888, 1817
TaaI	ACNGT	5 cut(s)	259, 814, 1301, 1771, 2290
TaiI	ACGT	3 cut(s)	954, 1477, 1927
TaqI	TCGA	3 cut(s)	809, 1370, 1570
TatI	WGTACW	1 cut(s)	1196
TauI	GCSGC	1 cut(s)	449
TfiI	GAWTC	7 cut(s)	278, 512, 737, 1169, 1271, 1283, 2485
TscAI	CASTG	8 cut(s)	163, 240, 609, 859, 981, 1306, 1582, 1716
TseFI	GTSAC	3 cut(s)	529, 844, 947
Tsp45I	GTSAC	3 cut(s)	529, 844, 947
TspDTI	ATGAA	7 cut(s)	17, 122, 678, 930, 1449, 1563, 1677
TspGWI	ACGGA	3 cut(s)	417, 559, 1822
TspRI	CASTG	8 cut(s)	163, 240, 609, 859, 981, 1306, 1582, 1716
VneI	GTGCAC	1 cut(s)	2211
VpaK11BI	GGWCC	7 cut(s)	134, 212, 458, 638, 1446, 1669, 1785
XapI	RAATTY	2 cut(s)	97, 817
XceI	RCATGY	4 cut(s)	307, 347, 482, 2189
XcmI	CCANNNNNNNNNTGG	3 cut(s)	144, 1371, 2146
XmaJI	CCTAGG	1 cut(s)	419
XmiI	GTMKAC	1 cut(s)	711
XmnI	GAANNNNTTC	2 cut(s)	1096, 1701
XspI	CTAG	6 cut(s)	57, 300, 420, 2100, 2460, 2499

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.