Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0002956

Physical Location & Seq

Genomic Coordinates Reverse (-)

69055504 .. 69066195

Gene Structure 10692 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG459300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2934 bp

ATGAAAAAGGGTCAAGAAACAACAGAGGTGATGGTGGAGGGTGAGAAGTCTGACTTGGAGAAAGCGAGAGTTCAAGGTTCCGTCGGAGACCTGCCTCCACTGATATCAATCAAACACAATTTCCTGCTCTCTATTCTACGACGCCCCGTTTCTGCTCCACCATCTGATCAAATAATGGCATCGCCAGCTTGGTTACCGCAGGATGTGCAGCCATCTGTATCTCAAGCCCCAGTTTCTGGTTCACCAGCAGGGGCTCCTTCAACTCCGACTGCAGCTCCACTTGCTTCAAGTGCGGGCACACCATCATTCTCGTACAGTGTGCCTCCAAATGCAAATATCGCTTTTGGAACCTCTCAGCAGCCATCAACTAGTCCTGGGACTAAGTTTAATCTCTCAGCATCTCCAATGGTTATTCAACCCCCAGTTCCTGGCTTGTCGTCTGCTGCCAGTCCCTCTTTCTCATACAACATTCCAAAATCTAATTATCCCTTCCCTAATAATCAGCTGTTTCAATCTGGCATGAATGTCTCACCTGCTATTGCACAAGAAACTGGGAATGCCTCATTGTCATCAACTAGCTCTCATTCTGGTTACTTGGCTGCTTCTACTTCTTCAAGTTCCACAATGGCTCTATCATCAGCTCCTAATGTGGGACCAACAACTTCATGGGTTCCCACGACCCCATCATTTAATATGACTCATGGAATGCCTGGAACTCCTGGTCCCTCTGGAACAGCCCACTCACTGCAAATGTCCTTTAATCTGACTGCACGTTCTGCACCTGTTGATTCTTCTTCTATAGCCCTTAGACCTAGCTTGCAAACACCTTCTGCTGGATCATCTGCAGTTCAGCCACAAGCAGGTGTTCCCTACCCATCTTTACCTGCTATGGCTGCTAACCCCCACGGAGTCTGGTTGCAGTCCCCACAGATGGAAGGCGTACCTAGGTCACCATTTCAGGCATATCCTGCTGCTTTTCCCGGTCCCTTTCCTTTGCCTACACGTGTTATGCCTCCCCCTTCTGTTCTTATACCTGATTCTCAGCCTCCTGGTGTCACTCCTGTGGGAAATACGGTAGCAACATCCTTGTCTTCCAGTTCTGGTCATCAACTTGCACCTTCTTCATGGATGCAGACAGAATTACCTCATCCTGGTATTGATGACAGGAAACATGCCCTTGAGGCAGGCAACCAACATATAGCTGCTGTCAATCAACAATTGGATGCTTGGACTGCCCACAAGGCAGAGACAGGAGTTGTATACTATTATAATGCTGTGACTGGTGAATCAACTTATGAGAAGCCTCCTGGATTTAAGGAGGAGCCTGATAAGGTCTCTGTGCAGCCAGTTCCAGTTTCAACGGTGAATTTGTCCGGAACTGATTGGGTATTGGTTACAACAAGTGATGGGAAAAAGTTTTACCATAATAGCAAAACCAAGGTTAGCAGCTGGCAGATCCCAAATGAAGTGATAGAAATGAGAAAGAAGCAGGATGGTGACACGCCAAAAGAACATCCAGAGTCAGTGCCAAATAATAGTCAAATGGTTGAAAAGGAATCTGCTCCAATCAGTTTGAGTGCTCCGGCCATTAGCACGGGTGGTCGTGAAGCCATGGCTTACAAGGCCTCAACTGTGCAGGGATCATCATCTGCACTCGACCTGATTAAAAAGAAGTTGCAGGATTCTGGAGCTCCTGTTACTTCATCACCAGTTTCAGGACCTTCAGAGTTAAATGGCTCAAGAGGAGTTGAGAGTACACCTAAGGTCCAGCAGAGTGAGAATAGCAAGGATAAGCTAAATGACACTAATGGGGATGGTAACATGTCTGACTCCTCATCAGATTCAGAGGATGCAGATAGTGGGCCAACAAAAGAGGAGGGTATTATTCAATTCAAGGAGATGCTCAAAGAACGGGGAGTGGCACCATTTTCAAAGTGGGAGAAGGAACTGCCAAAGATAGTGTTTGATCCACGATTTAAGGCCATTCCAAGTTATTCAAGGAGAGCCTTGTTTGAGCACTATGTTAAGACACGTGCAGAGGAGGAACGCAAGGAGAAACGAGCAGCTCAGAAGGCTGCAATAGAGGCATTTAAGCAGTTACTTGATGAAGCAGTGGAGGATATTGATGATACCACTGATTACCACAGTTTCAGAAAGAAATGGGGCAATGATCCACGCTTTGAGAATTTGGACCGCAAGGATCGAGAACATTTGTTGAATGAAAGAGTCCTTCCATTGAAGAAGGCTGCTGAAGAAAAGGCTGAAGCAGTGCGTGCTGCTGCTGCAACTAGTTTTAACTGCATGCTTCAAGAAAAAGGGGATATTACAGTTAATTCCCGTTGGTCCAGGGTGAAAGATAGTCTGCGGAATGACCCAAGGTACAAATCTGTTAAGCATGAGGATAGGGAAGTTTTATTCAACAAGTACATATCAGATCTCAAAGCCGTGGAAGAGGAAGCAGAACGAGAGGCAAAAGCTAAAAGGGATGAGCAGGCATCTTGGACAGAATCTAAGCCCAAGTTGGAGAAGGACCCACAAAGGCGTGCAGCCAACCCTGATTTAAATTCATCTGACATGGAAAAGCTCTTCCGCGACCACATAAAGATGCTGCATGAGCGATGTGCACACGAGTTTAGAGCCCTGTTGGCTGAAGTGTTGACTACAGAAGCAGCCTCCCAAGAGACAGAGGATGGAAAAACAGTTCTTAACTCATGGTCAACAGCTAAACGTTTTCTAAAGTCTGATCCCAGATATAACAAGATGCCACGAAAAGATAGGGAAGTTGTGTGGCACCGATATGTGGAGGAGATGCTGCGGAAGCAAAAGTCATCACTTGATCATAGGGAAGACAGGAAATCAGATGCTAAAAGCAGAAGCTCTGCCGATGCTGGAAGGACCACCTTCGGATCAAGGGGTGCCCATGACCGTAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0001085 GO:0001103 GO:0003674 GO:0003712 GO:0003713 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045892 GO:0045934 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0065007 GO:0070013 GO:0070491 GO:0071704 GO:0080090 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03040 ko04212 map03040 map04212

Pfam Domains

Protein Families

FF WW

Protein Analysis

977

Amino Acids

106.37

Weight (kDa)

7.11

Isoelectric Point (pI)

56.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WW	PF00397	409 - 435	7.5e-08	WW domain
WW_TCERG1	PF23517	450 - 492	3.1e-09	TCERG1 WW domain
WW	PF00397	460 - 487	1.4e-06	WW domain
FF	PF01846	630 - 674	2.7e-12	FF domain
FF	PF01846	693 - 741	1.1e-08	FF domain
FF	PF01846	763 - 809	2.2e-11	FF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002956)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G19840
fragaria_vesca	FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700
malus_domestica	MD13G1007900.v1.1 MD13G1008000.v1.1 MD16G1000200.v1.1
prunus_persica	Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1
pyrus_communis	pycom13g00650 pycom16g00500 pycom16g00510 pycom16g00520
rosa_chinensis	RchiOBHm_Chr4g0446421
rosa_laevigata	RLG00000005662
rosa_multiflora	Rmu_co8175444.1_g000001 Rmu_sc0008360.1_g000005 Rmu_sc0018208.1_g000001 Rmu_sc0025430.1_g000001
rosa_roxburghii	Rroxscaffold_5G00386860
rosa_rugosa	Rorug04G0371200
rosa_samantha	Rh4AG432800 Rh4BG441900 Rh4CG459300 Rh4DG440700
rosa_wichuraiana	Rw4G036970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1269
AarI	CACCTGC	2 cut(s)	541, 851
Acc36I	ACCTGC	4 cut(s)	99, 541, 851, 892
AccB1I	GGYRCC	3 cut(s)	1921, 2790, 2915
AccB7I	CCANNNNNTGG	2 cut(s)	236, 428
AccI	GTMKAC	1 cut(s)	1260
AccII	CGCG	1 cut(s)	2592
AccIII	TCCGGA	1 cut(s)	1373
AciI	CCGC	6 cut(s)	197, 293, 2194, 2365, 2590, 2815
AclI	AACGTT	1 cut(s)	2728
AclWI	GGATC	8 cut(s)	844, 1450, 1648, 1961, 2165, 2208, 2738, 2914
AcoI	YGGCCR	1 cut(s)	1584
AcsI	RAATTY	3 cut(s)	1366, 2185, 2563
AcuI	CTGAAG	4 cut(s)	1707, 2271, 2283, 2670
AcvI	CACGTG	2 cut(s)	1004, 2033
AcyI	GRCGYC	1 cut(s)	142
AfaI	GTAC	5 cut(s)	314, 942, 1756, 2381, 2426
AfiI	CCNNNNNNNGG	9 cut(s)	236, 428, 650, 833, 860, 931, 1151, 1715, 2800
AflIII	ACRYGT	4 cut(s)	1001, 1003, 1821, 2030
AhlI	ACTAGT	2 cut(s)	368, 2288
AjnI	CCWGG	8 cut(s)	373, 427, 709, 718, 1048, 1150, 1306, 2345
AjuI	GAANNNNNNNTTGG	2 cut(s)	38, 70
AleI	CACNNNNGTG	1 cut(s)	1061
AloI	GAACNNNNNNTCC	4 cut(s)	706, 738, 2685, 2717
Alw21I	GWGCWC	4 cut(s)	1582, 1693, 2019, 2626
Alw26I	GTCTC	5 cut(s)	81, 532, 1241, 1339, 2675
Alw44I	GTGCAC	1 cut(s)	2622
AlwI	GGATC	8 cut(s)	844, 1450, 1648, 1961, 2165, 2208, 2738, 2914
AlwNI	CAGNNNCTG	2 cut(s)	236, 428
Aor13HI	TCCGGA	1 cut(s)	1373
AoxI	GGCC	4 cut(s)	1584, 1623, 1862, 1980
ApaLI	GTGCAC	1 cut(s)	2622
ApoI	RAATTY	3 cut(s)	1366, 2185, 2563
AspA2I	CCTAGG	1 cut(s)	944
AsuC2I	CCSGG	1 cut(s)	981
AvaII	GGWCC	9 cut(s)	653, 722, 983, 1718, 1765, 2191, 2343, 2530, 2895
AvrII	CCTAGG	1 cut(s)	944
AxyI	CCTNAGG	1 cut(s)	1761
BaeGI	GKGCMC	3 cut(s)	299, 2626, 2920
BanI	GGYRCC	3 cut(s)	1921, 2790, 2915
BanII	GRGCYC	3 cut(s)	256, 1693, 2641
BauI	CACGAG	1 cut(s)	2627
BbrPI	CACGTG	2 cut(s)	1004, 2033
BbsI	GAAGAC	2 cut(s)	1083, 2853
Bbv12I	GWGCWC	4 cut(s)	1582, 1693, 2019, 2626
BceAI	ACGGC	1 cut(s)	2429
BciT130I	CCWGG	8 cut(s)	375, 429, 711, 720, 1050, 1152, 1308, 2347
BclI	TGATCA	2 cut(s)	166, 2836
BcnI	CCSGG	1 cut(s)	981
BcoDI	GTCTC	5 cut(s)	81, 532, 1241, 1339, 2675
BcuI	ACTAGT	2 cut(s)	368, 2288
BfaI	CTAG	5 cut(s)	369, 576, 813, 945, 2289
BfmI	CTRYAG	4 cut(s)	270, 798, 843, 2661
BfuAI	ACCTGC	4 cut(s)	99, 541, 851, 892
BglI	GCCNNNNNGGC	3 cut(s)	1181, 1241, 2645
BglII	AGATCT	1 cut(s)	2434
BlnI	CCTAGG	1 cut(s)	944
Bme1390I	CCNGG	9 cut(s)	375, 429, 711, 720, 981, 1050, 1152, 1308, 2347
Bme18I	GGWCC	9 cut(s)	653, 722, 983, 1718, 1765, 2191, 2343, 2530, 2895
BmrFI	CCNGG	9 cut(s)	375, 429, 711, 720, 981, 1050, 1152, 1308, 2347
BmrI	ACTGGG	3 cut(s)	224, 416, 561
BmuI	ACTGGG	3 cut(s)	224, 416, 561
BpiI	GAAGAC	2 cut(s)	1083, 2853
BpmI	CTGGAG	1 cut(s)	1707
BpuEI	CTTGAG	3 cut(s)	207, 1199, 1723
BpuMI	CCSGG	1 cut(s)	981
BsaAI	YACGTR	2 cut(s)	1004, 2033
BsaBI	GATNNNNATC	2 cut(s)	107, 1645
BsaHI	GRCGYC	1 cut(s)	142
BsaI	GGTCTC	2 cut(s)	81, 1339
BsaJI	CCNNGG	8 cut(s)	374, 904, 944, 1437, 1611, 2346, 2375, 2445
BsaWI	WCCGGW	1 cut(s)	1373
BsaXI	ACNNNNNCTCC	4 cut(s)	259, 289, 1680, 1710
Bsc4I	CCNNNNNNNGG	9 cut(s)	236, 428, 650, 833, 860, 931, 1151, 1715, 2800
Bse1I	ACTGG	9 cut(s)	230, 422, 447, 556, 1095, 1285, 1346, 1352, 1709
Bse21I	CCTNAGG	1 cut(s)	1761
Bse3DI	GCAATG	1 cut(s)	2173
Bse8I	GATNNNNATC	2 cut(s)	107, 1645
BseAI	TCCGGA	1 cut(s)	1373
BseBI	CCWGG	8 cut(s)	375, 429, 711, 720, 1050, 1152, 1308, 2347
BseDI	CCNNGG	8 cut(s)	374, 904, 944, 1437, 1611, 2346, 2375, 2445
BseJI	GATNNNNATC	2 cut(s)	107, 1645
BseLI	CCNNNNNNNGG	9 cut(s)	236, 428, 650, 833, 860, 931, 1151, 1715, 2800
BseMI	GCAATG	1 cut(s)	2173
BseMII	CTCAG	4 cut(s)	368, 408, 1055, 2081
BseNI	ACTGG	9 cut(s)	230, 422, 447, 556, 1095, 1285, 1346, 1352, 1709
BseRI	GAGGAG	6 cut(s)	1334, 1758, 1822, 1889, 2054, 2819
BseSI	GKGCMC	3 cut(s)	299, 2626, 2920
BsgI	GTGCAG	8 cut(s)	227, 753, 762, 1361, 1635, 1655, 2055, 2565
Bsh1236I	CGCG	1 cut(s)	2592
BshFI	GGCC	4 cut(s)	1586, 1625, 1864, 1982
BshNI	GGYRCC	3 cut(s)	1921, 2790, 2915
BsiHKAI	GWGCWC	4 cut(s)	1582, 1693, 2019, 2626
BsiSI	CCGG	3 cut(s)	981, 1374, 1583
BslFI	GGGAC	6 cut(s)	391, 435, 666, 708, 907, 969
BslI	CCNNNNNNNGG	9 cut(s)	236, 428, 650, 833, 860, 931, 1151, 1715, 2800
BsmAI	GTCTC	5 cut(s)	81, 532, 1241, 1339, 2675
BsmFI	GGGAC	6 cut(s)	391, 435, 666, 708, 907, 969
BsmI	GAATGC	2 cut(s)	562, 711
BsnI	GGCC	4 cut(s)	1586, 1625, 1864, 1982
Bso31I	GGTCTC	2 cut(s)	81, 1339
Bsp1286I	GDGCHC	8 cut(s)	256, 299, 1582, 1693, 2019, 2626, 2641, 2920
Bsp13I	TCCGGA	1 cut(s)	1373
Bsp19I	CCATGG	1 cut(s)	1611
BspACI	CCGC	6 cut(s)	197, 293, 2194, 2365, 2590, 2815
BspANI	GGCC	4 cut(s)	1586, 1625, 1864, 1982
BspCNI	CTCAG	4 cut(s)	367, 407, 1054, 2080
BspEI	TCCGGA	1 cut(s)	1373
BspFNI	CGCG	1 cut(s)	2592
BspMAI	CTGCAG	2 cut(s)	274, 847
BspMI	ACCTGC	4 cut(s)	99, 541, 851, 892
BspPI	GGATC	8 cut(s)	844, 1450, 1648, 1961, 2165, 2208, 2738, 2914
BspQI	GCTCTTC	1 cut(s)	2591
BspT107I	GGYRCC	3 cut(s)	1921, 2790, 2915
BspTNI	GGTCTC	2 cut(s)	81, 1339
BsrDI	GCAATG	1 cut(s)	2173
BsrI	ACTGG	9 cut(s)	230, 422, 447, 556, 1095, 1285, 1346, 1352, 1709
BssECI	CCNNGG	8 cut(s)	374, 904, 944, 1437, 1611, 2346, 2375, 2445
BssNAI	GTATAC	1 cut(s)	1261
BssNI	GRCGYC	1 cut(s)	142
BssSI	CACGAG	1 cut(s)	2627
BssT1I	CCWWGG	4 cut(s)	944, 1437, 1611, 2375
Bst1107I	GTATAC	1 cut(s)	1261
Bst2BI	CACGAG	1 cut(s)	2627
Bst2UI	CCWGG	8 cut(s)	375, 429, 711, 720, 1050, 1152, 1308, 2347
Bst4CI	ACNGT	8 cut(s)	317, 1075, 1363, 1633, 2147, 2329, 2701, 2927
Bst6I	CTCTTC	2 cut(s)	2445, 2591
BstACI	GRCGYC	1 cut(s)	142
BstAPI	GCANNNNNTGC	4 cut(s)	205, 776, 968, 2273
BstBAI	YACGTR	2 cut(s)	1004, 2033
BstC8I	GCNNGC	9 cut(s)	186, 295, 818, 1186, 1451, 2274, 2303, 2493, 2544
BstDEI	CTNAG	8 cut(s)	354, 381, 394, 806, 1041, 1761, 2067, 2511
BstDSI	CCRYGG	3 cut(s)	904, 1611, 2445
BstEII	GGTNACC	2 cut(s)	192, 948
BstFNI	CGCG	1 cut(s)	2592
BstMAI	GTCTC	5 cut(s)	81, 532, 1241, 1339, 2675
BstNI	CCWGG	8 cut(s)	375, 429, 711, 720, 1050, 1152, 1308, 2347
BstNSI	RCATGY	3 cut(s)	1175, 1825, 2305
BstPI	GGTNACC	2 cut(s)	192, 948
BstSCI	CCNGG	9 cut(s)	373, 427, 709, 718, 979, 1048, 1150, 1306, 2345
BstSFI	CTRYAG	4 cut(s)	270, 798, 843, 2661
BstSLI	GKGCMC	3 cut(s)	299, 2626, 2920
BstUI	CGCG	1 cut(s)	2592
BstV2I	GAAGAC	2 cut(s)	1083, 2853
BstX2I	RGATCY	2 cut(s)	1455, 2434
BstYI	RGATCY	2 cut(s)	1455, 2434
BstZ17I	GTATAC	1 cut(s)	1261
Bsu36I	CCTNAGG	1 cut(s)	1761
BsuRI	GGCC	4 cut(s)	1586, 1625, 1864, 1982
BtgI	CCRYGG	3 cut(s)	904, 1611, 2445
BtgZI	GCGATG	2 cut(s)	165, 2632
BtsI	GCAGTG	3 cut(s)	743, 2118, 2274
BtsIMutI	CAGTG	7 cut(s)	98, 322, 743, 1530, 2118, 2133, 2274
BveI	ACCTGC	4 cut(s)	99, 541, 851, 892
Cac8I	GCNNGC	9 cut(s)	186, 295, 818, 1186, 1451, 2274, 2303, 2493, 2544
CaiI	CAGNNNCTG	2 cut(s)	236, 428
CseI	GACGC	1 cut(s)	150
Csp6I	GTAC	5 cut(s)	313, 941, 1755, 2380, 2425
CviQI	GTAC	5 cut(s)	313, 941, 1755, 2380, 2425
DdeI	CTNAG	8 cut(s)	354, 381, 394, 806, 1041, 1761, 2067, 2511
DraI	TTTAAA	1 cut(s)	2562
EaeI	YGGCCR	1 cut(s)	1584
Eam1104I	CTCTTC	2 cut(s)	2445, 2591
EarI	CTCTTC	2 cut(s)	2445, 2591
Ecl136II	GAGCTC	1 cut(s)	1691
Eco130I	CCWWGG	4 cut(s)	944, 1437, 1611, 2375
Eco147I	AGGCCT	1 cut(s)	1625
Eco24I	GRGCYC	3 cut(s)	256, 1693, 2641
Eco31I	GGTCTC	2 cut(s)	81, 1339
Eco32I	GATATC	1 cut(s)	105
Eco47I	GGWCC	9 cut(s)	653, 722, 983, 1718, 1765, 2191, 2343, 2530, 2895
Eco53kI	GAGCTC	1 cut(s)	1691
Eco57I	CTGAAG	4 cut(s)	1707, 2271, 2283, 2670
Eco72I	CACGTG	2 cut(s)	1004, 2033
Eco81I	CCTNAGG	1 cut(s)	1761
Eco91I	GGTNACC	2 cut(s)	192, 948
EcoICRI	GAGCTC	1 cut(s)	1691
EcoO109I	RGGNCCY	2 cut(s)	1718, 2530
EcoO65I	GGTNACC	2 cut(s)	192, 948
EcoRII	CCWGG	8 cut(s)	373, 427, 709, 718, 1048, 1150, 1306, 2345
EcoRV	GATATC	1 cut(s)	105
EcoT14I	CCWWGG	4 cut(s)	944, 1437, 1611, 2375
EcoT38I	GRGCYC	3 cut(s)	256, 1693, 2641
ErhI	CCWWGG	4 cut(s)	944, 1437, 1611, 2375
FalI	AAGNNNNNCTT	2 cut(s)	38, 70
FaqI	GGGAC	6 cut(s)	391, 435, 666, 708, 907, 969
FauI	CCCGC	1 cut(s)	286
FbaI	TGATCA	2 cut(s)	166, 2836
FblI	GTMKAC	1 cut(s)	1260
FriOI	GRGCYC	3 cut(s)	256, 1693, 2641
FspBI	CTAG	5 cut(s)	369, 576, 813, 945, 2289
GsuI	CTGGAG	1 cut(s)	1707
HaeIII	GGCC	4 cut(s)	1586, 1625, 1864, 1982
HapII	CCGG	3 cut(s)	981, 1374, 1583
HgaI	GACGC	1 cut(s)	150
Hin1I	GRCGYC	1 cut(s)	142
HincII	GTYRAC	2 cut(s)	2658, 2718
HindII	GTYRAC	2 cut(s)	2658, 2718
HpaII	CCGG	3 cut(s)	981, 1374, 1583
Hpy166II	GTNNAC	6 cut(s)	242, 1261, 1757, 2624, 2658, 2718
Hpy8I	GTNNAC	6 cut(s)	242, 1261, 1757, 2624, 2658, 2718
Hpy99I	CGWCG	2 cut(s)	86, 144
HpyCH4III	ACNGT	8 cut(s)	317, 1075, 1363, 1633, 2147, 2329, 2701, 2927
HpyCH4IV	ACGT	4 cut(s)	772, 1003, 2032, 2728
HpyF3I	CTNAG	8 cut(s)	354, 381, 394, 806, 1041, 1761, 2067, 2511
HpySE526I	ACGT	4 cut(s)	772, 1003, 2032, 2728
Hsp92I	GRCGYC	1 cut(s)	142
Kpn2I	TCCGGA	1 cut(s)	1373
Ksp22I	TGATCA	2 cut(s)	166, 2836
LguI	GCTCTTC	1 cut(s)	2591
LmnI	GCTCC	9 cut(s)	160, 259, 280, 646, 1321, 1567, 1585, 1688, 1696
MaeI	CTAG	5 cut(s)	369, 576, 813, 945, 2289
MaeII	ACGT	4 cut(s)	772, 1003, 2032, 2728
MfeI	CAATTG	1 cut(s)	1217
MflI	RGATCY	2 cut(s)	1455, 2434
MhlI	GDGCHC	8 cut(s)	256, 299, 1582, 1693, 2019, 2626, 2641, 2920
MluCI	AATT	9 cut(s)	118, 481, 1139, 1217, 1366, 1889, 2185, 2332, 2563
MlyI	GAGTC	5 cut(s)	691, 918, 1529, 1823, 2235
MmeI	TCCRAC	3 cut(s)	64, 290, 2502
MroI	TCCGGA	1 cut(s)	1373
MslI	CAYNNNNRTG	3 cut(s)	1061, 2603, 2796
MspA1I	CMGCKG	2 cut(s)	505, 1449
MspI	CCGG	3 cut(s)	981, 1374, 1583
MspR9I	CCNGG	9 cut(s)	375, 429, 711, 720, 981, 1050, 1152, 1308, 2347
MunI	CAATTG	1 cut(s)	1217
Mva1269I	GAATGC	2 cut(s)	562, 711
MvaI	CCWGG	8 cut(s)	375, 429, 711, 720, 1050, 1152, 1308, 2347
MvnI	CGCG	1 cut(s)	2592
NciI	CCSGG	1 cut(s)	981
NcoI	CCATGG	1 cut(s)	1611
NmuCI	GTSAC	4 cut(s)	948, 1054, 1276, 1496
NspI	RCATGY	3 cut(s)	1175, 1825, 2305
OliI	CACNNNNGTG	1 cut(s)	1061
PaeI	GCATGC	1 cut(s)	2305
PaqCI	CACCTGC	2 cut(s)	541, 851
PceI	AGGCCT	1 cut(s)	1625
PciI	ACATGT	1 cut(s)	1821
PciSI	GCTCTTC	1 cut(s)	2591
PctI	GAATGC	2 cut(s)	562, 711
PfeI	GAWTC	7 cut(s)	788, 1037, 1286, 1556, 1682, 1841, 2507
PflMI	CCANNNNNTGG	2 cut(s)	236, 428
PfoI	TCCNGGA	1 cut(s)	1306
PleI	GAGTC	5 cut(s)	691, 917, 1528, 1823, 2234
PmaCI	CACGTG	2 cut(s)	1004, 2033
PmlI	CACGTG	2 cut(s)	1004, 2033
PpsI	GAGTC	5 cut(s)	691, 917, 1528, 1823, 2234
Ppu21I	YACGTR	2 cut(s)	1004, 2033
PpuMI	RGGWCCY	2 cut(s)	1718, 2530
PscI	ACATGT	1 cut(s)	1821
PsiI	TTATAA	1 cut(s)	1269
Psp124BI	GAGCTC	1 cut(s)	1693
Psp1406I	AACGTT	1 cut(s)	2728
Psp5II	RGGWCCY	2 cut(s)	1718, 2530
Psp6I	CCWGG	8 cut(s)	373, 427, 709, 718, 1048, 1150, 1306, 2345
PspCI	CACGTG	2 cut(s)	1004, 2033
PspEI	GGTNACC	2 cut(s)	192, 948
PspGI	CCWGG	8 cut(s)	373, 427, 709, 718, 1048, 1150, 1306, 2345
PspPPI	RGGWCCY	2 cut(s)	1718, 2530
PstI	CTGCAG	2 cut(s)	274, 847
PstNI	CAGNNNCTG	2 cut(s)	236, 428
PsuI	RGATCY	2 cut(s)	1455, 2434
PvuII	CAGCTG	2 cut(s)	505, 1449
RsaI	GTAC	5 cut(s)	314, 942, 1756, 2381, 2426
RsaNI	GTAC	5 cut(s)	313, 941, 1755, 2380, 2425
RseI	CAYNNNNRTG	3 cut(s)	1061, 2603, 2796
SacI	GAGCTC	1 cut(s)	1693
SapI	GCTCTTC	1 cut(s)	2591
SchI	GAGTC	5 cut(s)	691, 918, 1529, 1823, 2235
ScrFI	CCNGG	9 cut(s)	375, 429, 711, 720, 981, 1050, 1152, 1308, 2347
SduI	GDGCHC	8 cut(s)	256, 299, 1582, 1693, 2019, 2626, 2641, 2920
SfcI	CTRYAG	4 cut(s)	270, 798, 843, 2661
SinI	GGWCC	9 cut(s)	653, 722, 983, 1718, 1765, 2191, 2343, 2530, 2895
SmiI	ATTTAAAT	1 cut(s)	2562
SmiMI	CAYNNNNRTG	3 cut(s)	1061, 2603, 2796
SmlI	CTYRAG	3 cut(s)	222, 1178, 1738
SmoI	CTYRAG	3 cut(s)	222, 1178, 1738
SpeI	ACTAGT	2 cut(s)	368, 2288
SphI	GCATGC	1 cut(s)	2305
Sse9I	AATT	9 cut(s)	118, 481, 1139, 1217, 1366, 1889, 2185, 2332, 2563
SseBI	AGGCCT	1 cut(s)	1625
SsiI	CCGC	6 cut(s)	197, 293, 2194, 2365, 2590, 2815
SspMI	CTAG	5 cut(s)	369, 576, 813, 945, 2289
SstI	GAGCTC	1 cut(s)	1693
StuI	AGGCCT	1 cut(s)	1625
StyD4I	CCNGG	9 cut(s)	373, 427, 709, 718, 979, 1048, 1150, 1306, 2345
StyI	CCWWGG	4 cut(s)	944, 1437, 1611, 2375
SwaI	ATTTAAAT	1 cut(s)	2562
TaaI	ACNGT	8 cut(s)	317, 1075, 1363, 1633, 2147, 2329, 2701, 2927
TaiI	ACGT	4 cut(s)	775, 1006, 2035, 2731
TaqI	TCGA	2 cut(s)	1656, 2203
TasI	AATT	9 cut(s)	118, 481, 1139, 1217, 1366, 1889, 2185, 2332, 2563
TatI	WGTACW	2 cut(s)	1754, 2424
TfiI	GAWTC	7 cut(s)	788, 1037, 1286, 1556, 1682, 1841, 2507
TscAI	CASTG	7 cut(s)	105, 322, 750, 1530, 2118, 2140, 2274
TseFI	GTSAC	4 cut(s)	948, 1054, 1276, 1496
Tsp45I	GTSAC	4 cut(s)	948, 1054, 1276, 1496
TspDTI	ATGAA	9 cut(s)	17, 536, 654, 1113, 1479, 1692, 2121, 2235, 2556
TspGWI	ACGGA	2 cut(s)	70, 921
TspRI	CASTG	7 cut(s)	105, 322, 750, 1530, 2118, 2140, 2274
Van91I	CCANNNNNTGG	2 cut(s)	236, 428
VneI	GTGCAC	1 cut(s)	2622
VpaK11BI	GGWCC	9 cut(s)	653, 722, 983, 1718, 1765, 2191, 2343, 2530, 2895
XapI	RAATTY	3 cut(s)	1366, 2185, 2563
XceI	RCATGY	3 cut(s)	1175, 1825, 2305
XcmI	CCANNNNNNNNNTGG	2 cut(s)	663, 1932
XmaJI	CCTAGG	1 cut(s)	944
XmiI	GTMKAC	1 cut(s)	1260
XspI	CTAG	5 cut(s)	369, 576, 813, 945, 2289

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.