Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0002956

Physical Location & Seq

Genomic Coordinates Reverse (-)

64234564 .. 64243568

Gene Structure 9005 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG440700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2910 bp

ATGGCATCGCCAGCTTGGTTACCGCAGGATGTGCAGCCATCTGTATCTCAAGCCCCCGTTTCTGGTTCACCAGCAGGGGCTCCTTCAACTCCGACTGCAGCTCCACTTGCTTCAAGTGCGGGCACACCGTCATTCTCGTACAGTGTGCCTCCAAATGCAAATATCACTTTTGGAACCTCTCAGCAGCCATCAACTAGTCCTGGGACTAAGTTTAATCTCTCAGCATCTCCAATGGTTATTCAACCCCCAGTTCCTGGCTTGTCATCTGCTGCCAGTCCCTCTTTCTCATACAACATTCCAAAATCTAATTATCCCTTCCCTAATAATCAGCTGTTTCAATCTGGCATGCAGAATGTCTCACCTGCTATTGCACAAGAAACTGGGAATGCCTCATTGTCATCAACTAGCTCTCATTCTGGTTACTTGGCTGCTTCTACGTCTTCAAGTTCCACAATGGTTCTATCATCAGCTCCTAATGTGGGACCAACAACTTCATGGGTTCCCACTACCCCATCATTTAATATGACTCATGGAATGCCTGGAACTCCTGGTCCCCCTGGAACAGCCCACTCACTGCAAATGTCCTTTAATCTGACTGCACGATCTGCACCTGTTGATTCTTCTTCTATAGCCCTTAGACCTAGCTTGCAAACACCTTCTGCTGGATCATCTGCAGTTCTGCCACAAGCAGGTGCTCCCTACCCATCTTTACCTGCTATGGCTGCTAATCCCCACGGAGTCTGGTTGCAGTCCCCACAGATGGAAGGCATACCTAGGTCACCATTTCAGGCATATCCTGCTGCTTTTCCCGGTCCCTTTCCTTTGCCTACACGTGTTATGCCTCCCCCTTCTGTTCTTATACCTGATTCTCAGCCTCCTGGTGTCACTCCTGTGGGAAATACGGTAGCAACATCCTTGTCTTCCAGTTCTGGTCATCAACTTGCACCTTCTTCATGGATGCAGACAGAATTACCTCATCCTGGTATTGATGACAGGAAACATGCCCTTGAGGCAGGCAACCAACATATAGCTGCTGTCAATCAACAATTGGATGCTTGGACTGCCCACAAGACAGAGACAGGAGTTGTATACTATTATAATGCTGTGACTGGTGAATCAACTTATGAGAAGCCTCCTGGATTTAAGGAGGAGCCTGATAAGGTCTCTGTGCAGCCAGTTCCAGTTTCAACGGTGAATTTGTCCGGAACTGATTGGGTATTGGTTACAACGAGTGATGGGAAAAAGTTTTACCATAATAGCAAAACCAAGGTTAGCAGCTGGCAGATCCCAAATGAAGTGATAGAAATGAGAAAGAAGCAGGATGGTGACACGCCAAAAGAACATCCAGAGTCAGTGCCAAATAATAGTCAAATGGTTGAAAAGGAATCTGCTCCAATCAGTTTGAGTGCTCCAGCCATTAGCACGGGTGGTCGTGAAGCCATGGCTTACAAGGCCTCAACTGTGCAGGGATCATCATCTGCACTCGACCTGATTAAAAAGAAGTTGCAGGATTCTGGAGCTCCTGTTACTTCATCACCAGTTTCAGGACCTTCAGAGTTAAATGGCTCAAGAGGAGTTGAGAGTACACCTAAGGTCCAGCAGAGTGAGAATAGCAAGGATAAGCTAAATGACACTAATGGGGATGGTAACATGTCTGACTCCTCATCAGATTCAGAGGATGCAGATAGTGGGCCAACAAAAGAGGAGGGTATTATTCAATTCAAGGAGATGCTCAAAGAACGGGGAGTGGCACCATTTTCAAAGTGGGAGAAGGAACTGCCAAAGATAGTGTTTGATCCACGATTTAAGGCCATTCCAAGTTATTCAAGGAGAGCCTTGTTTGAGCACTATGTTAAGACACGTGCAGAGGAGGAACGCAAGGAGAAACGAGCAGCTCAGAAGGCTGCAATAGAGGCATTTAAGCAGTTACTTGATGAAGCAGTGGAGGATATTGATGATACCACTGATTACCACAGTTTCAGAAAGAAATGGGGCAATGATCCACGCTTTGAGAATTTGGACCGCAAGGATCGAGAACATTTGTTGAATGAAAGAGTCCTTCCATTGAAGAAGGCTGCTGAAGAAAAGGCTGAAGCAGTGCGTGCTGCTGCTGCAACTAGTTTTAAGTGCATGCTTCAAGAAAAAGGAGATATTACAGTTAATTCCCGTTGGTCCAGGGTGAAAGATAGTCTGCGGAATGACCCAAGGTACAAATCTGTTAAGCATGAGGATAGGGAAGTTTTATTCAACAAGTACATATCAGATCTCAAAGCTGTGGAAGAGGAAGCAGAACGAGAGGCAAAAGCTAAAAGGGATGAGCAGGATAAGTTAAGGGAAAGAGAGCGAGAACTACGCAAGCGGAAGGAAAGAGAAGAACAAGAAACAGAGAGGGTGCGATTGAAAGTTCGCAGGAAGGAGGCAGTGTCTTCTTTTCAAGCTTTACTTGTGGAAACAATTAAAGATCCTCAGGCATCTTGGACAGAATCTAAGCCCAAGTTGGAGAAGGACCCGCAAAGGCGTGCAGCCAACCCTGATCTGAATTCATCTGACATGGAAAAGCTCTTCCGCGACCACATAAAGATGCTGCATGAGCGATGTGCACACGAGTTTAGAGCCCTGTTGGCTGAAGTGTTGACTACAGAAGCAGCCTCCCAAGAGACAGAGGATGGAAAAACAGTTCTTAACTCATGGTCAACAGCTAAACGTTTTCTAAAGTCTGATCCCAGATATAACAAGATGCCACGAAAAGATAGGGAAGTCGTGTGGCACCGATATGCCGAGGAGATGCTGCGGAAGCAAATGTCATCACTTGATCATAGGGAAGACAGGAAATCAGATGCTAAAAGCAGAAGCTCTGCCGATGCTGGAAGGACCACCTTCGGATCAAGGGGTGCCCATGACCGTAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0001085 GO:0001103 GO:0003674 GO:0003712 GO:0003713 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045892 GO:0045934 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0065007 GO:0070013 GO:0070491 GO:0071704 GO:0080090 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03040 ko04212 map03040 map04212

Pfam Domains

Protein Families

FF WW

Protein Analysis

969

Amino Acids

106.28

Weight (kDa)

8.32

Isoelectric Point (pI)

57.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WW	PF00397	352 - 378	2.1e-08	WW domain
WW_TCERG1	PF23517	393 - 435	3.1e-09	TCERG1 WW domain
WW	PF00397	403 - 430	1.3e-06	WW domain
FF	PF01846	573 - 617	2.7e-12	FF domain
FF	PF01846	636 - 684	1.1e-08	FF domain
FF	PF01846	706 - 752	2.6e-12	FF domain
FF	PF01846	806 - 858	1.5e-06	FF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002956)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G19840
fragaria_vesca	FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700 FvH4_4g36700
malus_domestica	MD13G1007900.v1.1 MD13G1008000.v1.1 MD16G1000200.v1.1
prunus_persica	Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1 Prupe.1G345100_v2.0.a1
pyrus_communis	pycom13g00650 pycom16g00500 pycom16g00510 pycom16g00520
rosa_chinensis	RchiOBHm_Chr4g0446421
rosa_laevigata	RLG00000005662
rosa_multiflora	Rmu_co8175444.1_g000001 Rmu_sc0008360.1_g000005 Rmu_sc0018208.1_g000001 Rmu_sc0025430.1_g000001
rosa_roxburghii	Rroxscaffold_5G00386860
rosa_rugosa	Rorug04G0371200
rosa_samantha	Rh4AG432800 Rh4BG441900 Rh4CG459300 Rh4DG440700
rosa_wichuraiana	Rw4G036970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1098
AarI	CACCTGC	2 cut(s)	370, 680
Acc36I	ACCTGC	3 cut(s)	370, 680, 721
AccB1I	GGYRCC	3 cut(s)	1750, 2766, 2891
AccB7I	CCANNNNNTGG	1 cut(s)	254
AccI	GTMKAC	1 cut(s)	1089
AccII	CGCG	1 cut(s)	2568
AccIII	TCCGGA	1 cut(s)	1202
AciI	CCGC	8 cut(s)	23, 119, 2023, 2194, 2359, 2510, 2566, 2791
AclI	AACGTT	1 cut(s)	2704
AclWI	GGATC	9 cut(s)	673, 1279, 1477, 1790, 1994, 2037, 2456, 2714, 2890
AcsI	RAATTY	3 cut(s)	1195, 2014, 2539
AcuI	CTGAAG	4 cut(s)	1536, 2100, 2112, 2646
AcvI	CACGTG	2 cut(s)	833, 1862
AfaI	GTAC	4 cut(s)	140, 1585, 2210, 2255
AfiI	CCNNNNNNNGG	8 cut(s)	62, 254, 479, 662, 689, 760, 980, 1544
AflIII	ACRYGT	4 cut(s)	830, 832, 1650, 1859
AhlI	ACTAGT	2 cut(s)	194, 2117
AjnI	CCWGG	9 cut(s)	199, 253, 538, 547, 556, 877, 979, 1135, 2174
AleI	CACNNNNGTG	1 cut(s)	890
AloI	GAACNNNNNNTCC	4 cut(s)	535, 567, 2661, 2693
Alw21I	GWGCWC	5 cut(s)	697, 1411, 1522, 1848, 2602
Alw26I	GTCTC	4 cut(s)	361, 1070, 1168, 2651
Alw44I	GTGCAC	1 cut(s)	2598
AlwI	GGATC	9 cut(s)	673, 1279, 1477, 1790, 1994, 2037, 2456, 2714, 2890
AlwNI	CAGNNNCTG	1 cut(s)	254
Aor13HI	TCCGGA	1 cut(s)	1202
AoxI	GGCC	3 cut(s)	1452, 1691, 1809
ApaLI	GTGCAC	1 cut(s)	2598
ApoI	RAATTY	3 cut(s)	1195, 2014, 2539
AspA2I	CCTAGG	1 cut(s)	773
AsuC2I	CCSGG	1 cut(s)	810
AsuHPI	GGTGA	8 cut(s)	60, 351, 771, 1124, 1204, 1337, 1527, 2191
AvaII	GGWCC	9 cut(s)	482, 551, 812, 1547, 1594, 2020, 2172, 2506, 2871
AvrII	CCTAGG	1 cut(s)	773
AxyI	CCTNAGG	2 cut(s)	1590, 2466
BaeGI	GKGCMC	3 cut(s)	125, 2602, 2896
BanI	GGYRCC	3 cut(s)	1750, 2766, 2891
BanII	GRGCYC	3 cut(s)	82, 1522, 2617
BauI	CACGAG	1 cut(s)	2603
BbrPI	CACGTG	2 cut(s)	833, 1862
BbsI	GAAGAC	4 cut(s)	432, 912, 2418, 2829
Bbv12I	GWGCWC	5 cut(s)	697, 1411, 1522, 1848, 2602
BccI	CCATC	9 cut(s)	46, 196, 520, 712, 754, 1229, 1316, 1637, 2660
BciT130I	CCWGG	9 cut(s)	201, 255, 540, 549, 558, 879, 981, 1137, 2176
BclI	TGATCA	1 cut(s)	2812
BcnI	CCSGG	1 cut(s)	810
BcoDI	GTCTC	4 cut(s)	361, 1070, 1168, 2651
BcuI	ACTAGT	2 cut(s)	194, 2117
BfaI	CTAG	5 cut(s)	195, 405, 642, 774, 2118
BfmI	CTRYAG	4 cut(s)	96, 627, 672, 2637
BfuAI	ACCTGC	3 cut(s)	370, 680, 721
BglI	GCCNNNNNGGC	2 cut(s)	1010, 2621
BglII	AGATCT	1 cut(s)	2263
BlnI	CCTAGG	1 cut(s)	773
Bme18I	GGWCC	9 cut(s)	482, 551, 812, 1547, 1594, 2020, 2172, 2506, 2871
BmrI	ACTGGG	2 cut(s)	242, 390
BmuI	ACTGGG	2 cut(s)	242, 390
BpiI	GAAGAC	4 cut(s)	432, 912, 2418, 2829
BpmI	CTGGAG	2 cut(s)	1395, 1536
BpuEI	CTTGAG	3 cut(s)	33, 1028, 1552
BpuMI	CCSGG	1 cut(s)	810
BsaAI	YACGTR	2 cut(s)	833, 1862
BsaBI	GATNNNNATC	1 cut(s)	1474
BsaI	GGTCTC	1 cut(s)	1168
BsaJI	CCNNGG	9 cut(s)	200, 556, 733, 773, 1266, 1440, 2175, 2204, 2778
BsaWI	WCCGGW	1 cut(s)	1202
BsaXI	ACNNNNNCTCC	6 cut(s)	85, 115, 1509, 1539, 2408, 2438
Bsc4I	CCNNNNNNNGG	8 cut(s)	62, 254, 479, 662, 689, 760, 980, 1544
Bse1I	ACTGG	8 cut(s)	248, 273, 385, 924, 1114, 1175, 1181, 1538
Bse21I	CCTNAGG	2 cut(s)	1590, 2466
Bse3DI	GCAATG	1 cut(s)	2002
Bse8I	GATNNNNATC	1 cut(s)	1474
BseAI	TCCGGA	1 cut(s)	1202
BseBI	CCWGG	9 cut(s)	201, 255, 540, 549, 558, 879, 981, 1137, 2176
BseDI	CCNNGG	9 cut(s)	200, 556, 733, 773, 1266, 1440, 2175, 2204, 2778
BseJI	GATNNNNATC	1 cut(s)	1474
BseLI	CCNNNNNNNGG	8 cut(s)	62, 254, 479, 662, 689, 760, 980, 1544
BseMI	GCAATG	1 cut(s)	2002
BseMII	CTCAG	5 cut(s)	194, 234, 884, 1910, 2480
BseNI	ACTGG	8 cut(s)	248, 273, 385, 924, 1114, 1175, 1181, 1538
BseRI	GAGGAG	6 cut(s)	1163, 1587, 1651, 1718, 1883, 2795
BseSI	GKGCMC	3 cut(s)	125, 2602, 2896
BsgI	GTGCAG	8 cut(s)	53, 582, 591, 1190, 1464, 1484, 1884, 2541
Bsh1236I	CGCG	1 cut(s)	2568
BshFI	GGCC	3 cut(s)	1454, 1693, 1811
BshNI	GGYRCC	3 cut(s)	1750, 2766, 2891
BsiHKAI	GWGCWC	5 cut(s)	697, 1411, 1522, 1848, 2602
BsiSI	CCGG	2 cut(s)	810, 1203
BslFI	GGGAC	6 cut(s)	217, 261, 495, 537, 736, 798
BslI	CCNNNNNNNGG	8 cut(s)	62, 254, 479, 662, 689, 760, 980, 1544
BsmAI	GTCTC	4 cut(s)	361, 1070, 1168, 2651
BsmFI	GGGAC	6 cut(s)	217, 261, 495, 537, 736, 798
BsmI	GAATGC	2 cut(s)	391, 540
BsnI	GGCC	3 cut(s)	1454, 1693, 1811
Bso31I	GGTCTC	1 cut(s)	1168
Bsp1286I	GDGCHC	9 cut(s)	82, 125, 697, 1411, 1522, 1848, 2602, 2617, 2896
Bsp13I	TCCGGA	1 cut(s)	1202
Bsp19I	CCATGG	1 cut(s)	1440
BspACI	CCGC	8 cut(s)	23, 119, 2023, 2194, 2359, 2510, 2566, 2791
BspANI	GGCC	3 cut(s)	1454, 1693, 1811
BspCNI	CTCAG	5 cut(s)	193, 233, 883, 1909, 2479
BspEI	TCCGGA	1 cut(s)	1202
BspFNI	CGCG	1 cut(s)	2568
BspMAI	CTGCAG	2 cut(s)	100, 676
BspMI	ACCTGC	3 cut(s)	370, 680, 721
BspPI	GGATC	9 cut(s)	673, 1279, 1477, 1790, 1994, 2037, 2456, 2714, 2890
BspQI	GCTCTTC	1 cut(s)	2567
BspT107I	GGYRCC	3 cut(s)	1750, 2766, 2891
BspTNI	GGTCTC	1 cut(s)	1168
BsrDI	GCAATG	1 cut(s)	2002
BsrI	ACTGG	8 cut(s)	248, 273, 385, 924, 1114, 1175, 1181, 1538
BssECI	CCNNGG	9 cut(s)	200, 556, 733, 773, 1266, 1440, 2175, 2204, 2778
BssNAI	GTATAC	1 cut(s)	1090
BssSI	CACGAG	1 cut(s)	2603
BssT1I	CCWWGG	4 cut(s)	773, 1266, 1440, 2204
Bst1107I	GTATAC	1 cut(s)	1090
Bst2BI	CACGAG	1 cut(s)	2603
Bst2UI	CCWGG	9 cut(s)	201, 255, 540, 549, 558, 879, 981, 1137, 2176
Bst4CI	ACNGT	9 cut(s)	129, 143, 904, 1192, 1462, 1976, 2158, 2677, 2903
Bst6I	CTCTTC	2 cut(s)	2274, 2567
BstAPI	GCANNNNNTGC	4 cut(s)	31, 605, 797, 2102
BstBAI	YACGTR	2 cut(s)	833, 1862
BstDEI	CTNAG	9 cut(s)	180, 207, 220, 635, 870, 1590, 1896, 2466, 2487
BstDSI	CCRYGG	2 cut(s)	733, 1440
BstEII	GGTNACC	2 cut(s)	18, 777
BstFNI	CGCG	1 cut(s)	2568
BstMAI	GTCTC	4 cut(s)	361, 1070, 1168, 2651
BstNI	CCWGG	9 cut(s)	201, 255, 540, 549, 558, 879, 981, 1137, 2176
BstNSI	RCATGY	4 cut(s)	349, 1004, 1654, 2134
BstPI	GGTNACC	2 cut(s)	18, 777
BstSFI	CTRYAG	4 cut(s)	96, 627, 672, 2637
BstSLI	GKGCMC	3 cut(s)	125, 2602, 2896
BstUI	CGCG	1 cut(s)	2568
BstV2I	GAAGAC	4 cut(s)	432, 912, 2418, 2829
BstX2I	RGATCY	3 cut(s)	1284, 2263, 2461
BstYI	RGATCY	3 cut(s)	1284, 2263, 2461
BstZ17I	GTATAC	1 cut(s)	1090
Bsu36I	CCTNAGG	2 cut(s)	1590, 2466
BsuRI	GGCC	3 cut(s)	1454, 1693, 1811
BtgI	CCRYGG	2 cut(s)	733, 1440
BtgZI	GCGATG	1 cut(s)	2608
BtsI	GCAGTG	4 cut(s)	572, 1947, 2103, 2427
BtsIMutI	CAGTG	7 cut(s)	148, 572, 1359, 1947, 1962, 2103, 2427
BveI	ACCTGC	3 cut(s)	370, 680, 721
CaiI	CAGNNNCTG	1 cut(s)	254
Csp6I	GTAC	4 cut(s)	139, 1584, 2209, 2254
CviQI	GTAC	4 cut(s)	139, 1584, 2209, 2254
DdeI	CTNAG	9 cut(s)	180, 207, 220, 635, 870, 1590, 1896, 2466, 2487
Eam1104I	CTCTTC	2 cut(s)	2274, 2567
EarI	CTCTTC	2 cut(s)	2274, 2567
Ecl136II	GAGCTC	1 cut(s)	1520
Eco130I	CCWWGG	4 cut(s)	773, 1266, 1440, 2204
Eco147I	AGGCCT	1 cut(s)	1454
Eco24I	GRGCYC	3 cut(s)	82, 1522, 2617
Eco31I	GGTCTC	1 cut(s)	1168
Eco47I	GGWCC	9 cut(s)	482, 551, 812, 1547, 1594, 2020, 2172, 2506, 2871
Eco53kI	GAGCTC	1 cut(s)	1520
Eco57I	CTGAAG	4 cut(s)	1536, 2100, 2112, 2646
Eco72I	CACGTG	2 cut(s)	833, 1862
Eco81I	CCTNAGG	2 cut(s)	1590, 2466
Eco91I	GGTNACC	2 cut(s)	18, 777
EcoICRI	GAGCTC	1 cut(s)	1520
EcoO109I	RGGNCCY	2 cut(s)	1547, 2506
EcoO65I	GGTNACC	2 cut(s)	18, 777
EcoRI	GAATTC	1 cut(s)	2539
EcoRII	CCWGG	9 cut(s)	199, 253, 538, 547, 556, 877, 979, 1135, 2174
EcoT14I	CCWWGG	4 cut(s)	773, 1266, 1440, 2204
EcoT38I	GRGCYC	3 cut(s)	82, 1522, 2617
ErhI	CCWWGG	4 cut(s)	773, 1266, 1440, 2204
FalI	AAGNNNNNCTT	2 cut(s)	2427, 2459
FaqI	GGGAC	6 cut(s)	217, 261, 495, 537, 736, 798
FauI	CCCGC	2 cut(s)	112, 2517
FbaI	TGATCA	1 cut(s)	2812
FblI	GTMKAC	1 cut(s)	1089
FriOI	GRGCYC	3 cut(s)	82, 1522, 2617
FspBI	CTAG	5 cut(s)	195, 405, 642, 774, 2118
GsuI	CTGGAG	2 cut(s)	1395, 1536
HaeIII	GGCC	3 cut(s)	1454, 1693, 1811
HapII	CCGG	2 cut(s)	810, 1203
HincII	GTYRAC	2 cut(s)	2634, 2694
HindII	GTYRAC	2 cut(s)	2634, 2694
HindIII	AAGCTT	1 cut(s)	2436
HpaII	CCGG	2 cut(s)	810, 1203
HphI	GGTGA	8 cut(s)	60, 351, 771, 1124, 1204, 1337, 1527, 2191
Hpy166II	GTNNAC	6 cut(s)	68, 1090, 1586, 2600, 2634, 2694
Hpy188III	TCNNGA	8 cut(s)	1203, 1346, 1433, 1515, 1545, 1569, 2033, 2138
Hpy8I	GTNNAC	6 cut(s)	68, 1090, 1586, 2600, 2634, 2694
HpyCH4III	ACNGT	9 cut(s)	129, 143, 904, 1192, 1462, 1976, 2158, 2677, 2903
HpyCH4IV	ACGT	4 cut(s)	437, 832, 1861, 2704
HpyF3I	CTNAG	9 cut(s)	180, 207, 220, 635, 870, 1590, 1896, 2466, 2487
HpySE526I	ACGT	4 cut(s)	437, 832, 1861, 2704
Kpn2I	TCCGGA	1 cut(s)	1202
Ksp22I	TGATCA	1 cut(s)	2812
LguI	GCTCTTC	1 cut(s)	2567
LmnI	GCTCC	9 cut(s)	85, 106, 475, 700, 1150, 1396, 1414, 1517, 1525
MaeI	CTAG	5 cut(s)	195, 405, 642, 774, 2118
MaeII	ACGT	4 cut(s)	437, 832, 1861, 2704
MfeI	CAATTG	1 cut(s)	1046
MflI	RGATCY	3 cut(s)	1284, 2263, 2461
MhlI	GDGCHC	9 cut(s)	82, 125, 697, 1411, 1522, 1848, 2602, 2617, 2896
MluCI	AATT	9 cut(s)	307, 968, 1046, 1195, 1718, 2014, 2161, 2454, 2539
MlyI	GAGTC	5 cut(s)	520, 747, 1358, 1652, 2064
MmeI	TCCRAC	2 cut(s)	116, 2478
MroI	TCCGGA	1 cut(s)	1202
MslI	CAYNNNNRTG	3 cut(s)	890, 2579, 2772
MspA1I	CMGCKG	2 cut(s)	331, 1278
MspI	CCGG	2 cut(s)	810, 1203
MunI	CAATTG	1 cut(s)	1046
Mva1269I	GAATGC	2 cut(s)	391, 540
MvaI	CCWGG	9 cut(s)	201, 255, 540, 549, 558, 879, 981, 1137, 2176
MvnI	CGCG	1 cut(s)	2568
NciI	CCSGG	1 cut(s)	810
NcoI	CCATGG	1 cut(s)	1440
NmeAIII	GCCGAG	1 cut(s)	2803
NmuCI	GTSAC	4 cut(s)	777, 883, 1105, 1325
NspI	RCATGY	4 cut(s)	349, 1004, 1654, 2134
OliI	CACNNNNGTG	1 cut(s)	890
PaeI	GCATGC	2 cut(s)	349, 2134
PaqCI	CACCTGC	2 cut(s)	370, 680
PceI	AGGCCT	1 cut(s)	1454
PciI	ACATGT	1 cut(s)	1650
PciSI	GCTCTTC	1 cut(s)	2567
PctI	GAATGC	2 cut(s)	391, 540
PfeI	GAWTC	7 cut(s)	617, 866, 1115, 1385, 1511, 1670, 2483
PflMI	CCANNNNNTGG	1 cut(s)	254
PfoI	TCCNGGA	1 cut(s)	1135
PleI	GAGTC	5 cut(s)	520, 746, 1357, 1652, 2063
PmaCI	CACGTG	2 cut(s)	833, 1862
PmlI	CACGTG	2 cut(s)	833, 1862
PpsI	GAGTC	5 cut(s)	520, 746, 1357, 1652, 2063
Ppu21I	YACGTR	2 cut(s)	833, 1862
PpuMI	RGGWCCY	2 cut(s)	1547, 2506
PscI	ACATGT	1 cut(s)	1650
PsiI	TTATAA	1 cut(s)	1098
Psp124BI	GAGCTC	1 cut(s)	1522
Psp1406I	AACGTT	1 cut(s)	2704
Psp5II	RGGWCCY	2 cut(s)	1547, 2506
Psp6I	CCWGG	9 cut(s)	199, 253, 538, 547, 556, 877, 979, 1135, 2174
PspCI	CACGTG	2 cut(s)	833, 1862
PspEI	GGTNACC	2 cut(s)	18, 777
PspGI	CCWGG	9 cut(s)	199, 253, 538, 547, 556, 877, 979, 1135, 2174
PspPPI	RGGWCCY	2 cut(s)	1547, 2506
PstI	CTGCAG	2 cut(s)	100, 676
PstNI	CAGNNNCTG	1 cut(s)	254
PsuI	RGATCY	3 cut(s)	1284, 2263, 2461
PvuII	CAGCTG	2 cut(s)	331, 1278
RsaI	GTAC	4 cut(s)	140, 1585, 2210, 2255
RsaNI	GTAC	4 cut(s)	139, 1584, 2209, 2254
RseI	CAYNNNNRTG	3 cut(s)	890, 2579, 2772
SacI	GAGCTC	1 cut(s)	1522
SapI	GCTCTTC	1 cut(s)	2567
SchI	GAGTC	5 cut(s)	520, 747, 1358, 1652, 2064
SduI	GDGCHC	9 cut(s)	82, 125, 697, 1411, 1522, 1848, 2602, 2617, 2896
SfcI	CTRYAG	4 cut(s)	96, 627, 672, 2637
SinI	GGWCC	9 cut(s)	482, 551, 812, 1547, 1594, 2020, 2172, 2506, 2871
SmiMI	CAYNNNNRTG	3 cut(s)	890, 2579, 2772
SmlI	CTYRAG	3 cut(s)	48, 1007, 1567
SmoI	CTYRAG	3 cut(s)	48, 1007, 1567
SpeI	ACTAGT	2 cut(s)	194, 2117
SphI	GCATGC	2 cut(s)	349, 2134
Sse9I	AATT	9 cut(s)	307, 968, 1046, 1195, 1718, 2014, 2161, 2454, 2539
SseBI	AGGCCT	1 cut(s)	1454
SsiI	CCGC	8 cut(s)	23, 119, 2023, 2194, 2359, 2510, 2566, 2791
SspMI	CTAG	5 cut(s)	195, 405, 642, 774, 2118
SstI	GAGCTC	1 cut(s)	1522
StuI	AGGCCT	1 cut(s)	1454
StyI	CCWWGG	4 cut(s)	773, 1266, 1440, 2204
TaaI	ACNGT	9 cut(s)	129, 143, 904, 1192, 1462, 1976, 2158, 2677, 2903
TaiI	ACGT	4 cut(s)	440, 835, 1864, 2707
TaqI	TCGA	2 cut(s)	1485, 2032
TasI	AATT	9 cut(s)	307, 968, 1046, 1195, 1718, 2014, 2161, 2454, 2539
TatI	WGTACW	2 cut(s)	1583, 2253
TfiI	GAWTC	7 cut(s)	617, 866, 1115, 1385, 1511, 1670, 2483
TscAI	CASTG	7 cut(s)	148, 579, 1359, 1947, 1969, 2103, 2427
TseFI	GTSAC	4 cut(s)	777, 883, 1105, 1325
Tsp45I	GTSAC	4 cut(s)	777, 883, 1105, 1325
TspDTI	ATGAA	7 cut(s)	483, 942, 1308, 1521, 1950, 2064, 2532
TspGWI	ACGGA	1 cut(s)	750
TspRI	CASTG	7 cut(s)	148, 579, 1359, 1947, 1969, 2103, 2427
Van91I	CCANNNNNTGG	1 cut(s)	254
VneI	GTGCAC	1 cut(s)	2598
VpaK11BI	GGWCC	9 cut(s)	482, 551, 812, 1547, 1594, 2020, 2172, 2506, 2871
XapI	RAATTY	3 cut(s)	1195, 2014, 2539
XceI	RCATGY	4 cut(s)	349, 1004, 1654, 2134
XcmI	CCANNNNNNNNNTGG	2 cut(s)	492, 1761
XmaJI	CCTAGG	1 cut(s)	773
XmiI	GTMKAC	1 cut(s)	1089
XspI	CTAG	5 cut(s)	195, 405, 642, 774, 2118

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.