MD16G1001700.v1.1

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013278
Physical Location & Seq
Reverse (-)
102955 .. 106083
3129 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1001700.v1.1.491

Sequence Viewer

Length: 1887 bp
ATGACGAGGAACTGCAACCTTGCACCGTGCGTTTTGCTAGCCTTGCTACTACTCGGAATTCTCCACCTCGAATCAGCCACCGCGACAGACTTCACCTACGGCGAGGCCCTGGACAAGACCTTGATGTTTTTCGAGGCACAAAGGTCAGGTAAGTTGCCGGAAACTCAGCGTGTCAACTGGCGCGGCGACTCGGGACTCAAGGACGGCCTTGCTCAAGGGGTGAACTTGGTAGGAGGGTATTATGATGCTGGTGATCATGTGAAATTTGGACTGCCGATGGCGTTTACAGTGACAATGCTTTCATGGGGAGCTGTTGAATACAGAAAGGAAATTTCGGATTTAGCGCAACTGGGTCAAACCTTACGGGCCATCCGATGGGGCACTGACTACTTCATGAAAGCACATCCACAACCTAATGTTCTCTGGGGACAGGTAGGAGATGGGGACTCGGATCACTATTGCTGGGAGCGTGCAGAAGACATGACAACTCCAAGAACTGCTTACAAGACTGACGAAAGCCATCCCGGTTCGGACCTTGCCGGAGAAACTGCAGCCGCCTTGGCATCGGCCGCCATAGCTTTCAAGCCTTACAACTCTTCCTACTCTGCTCTCCTCTTAGTTCATGCAAAACAGCTCTTCACATTTGCGGATAGATTCAGAGGTCTATATGACGAGTCCATTGGCACTGCTAAGAATTTCTACACTTCATCAGGCTATTCGGATGAACTACTTTGGGCGGCGGCGTGGCTGTTCGAAGCGACGAACGATCAGTACTACCTCAAGTATGTGGTGGACAACGCCGTGTATATGGGCGGAACTGGATGGGCTGTCAAAGAATTCTCTTGGGATAACAAATATGCTGGTGTCCAAATCCTTCTCTCAAAGGTGTTGTTGGAGGGAAGAGGCGGTGCATACACTTCCACACTAAAGCAATATCAGGCCAAAGCAGATTACTTTGCATGTGCTTGTCTCCAGAAAAACGATGGCTACAATGTCCAGAAAACTCGAGGACTATTGTACGTGAGGGAATGGAACAACTTGCAGTACGTGACGTCAGCAACATTTCTCCTAGCCGTCTACTCCGATTATCTCTCCGCCGCAAACGCCAAGCTAACTTGTCCCGACGGCCAAGCCCAACCCCAAGAGCTCCTCAACTTCGCCAAGTCACAGGCGGACTACATACTCGGCAAAAACCCCAATTCCATGAGCTACATAGTTGGGATTGGACAAAATTATCCAGTCCATGTCCACCACAGAGGCGCTTCAATGCCCTCTGTTTTTACTCTCCACTCTGCGGTCGAATGTGTGCAAGGTTTTGAGACGTGGTACCAGCGCCCCGAGGCAAACCCCAACGTCATCTACGGTGCCCTTGTGGGAGGCCCTGACCAGAATGACAACTTCTCCGACGACCGCTCTAGTTATGAACAGACCGAGCCTACGCTTTCCGGCACCGGTCCCCTTGTTGGCCTCTTGTCTAAGTTGCAGAGTTTGAATGGCATCCATCCAAGTTATAATAAAGGCACCCCAAACTCCCAGCCAACCATACCAAGAGAAGGTGCCAATGACCATGAAGAACAAAATCCAGTACAAGCTCCAGAGACCCCTATCAAACTGATTCACTCAATCGGCAACACATGGACGGTTGGGAAAACAACATATTATCGCCACAAGGTGGTACTGAAGAACACATCGCAGAAACCGATATCGGATGTGAAGCTGGAGATTGAAGATCTCAAAGGATCTGTGTGGGGTCTCATCCCAACACCAGAAAAGAACATCTACGAGCTTCCTCAATGGCACAAGGTCTTGCAGCCTGGCTCTGAGTGCATTTTCATTTACGTCCAAGGTGGTTCTCAGGCAAACGTTTCCATCGTAAGCTACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
CBM49 Glyco_hydro_9

Protein Analysis

629

Amino Acids

69.58

Weight (kDa)

5.76

Isoelectric Point (pI)

30.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 33 - 491 8.1e-165 Glycosyl hydrolase family 9
CBM49 PF09478 537 - 615 5.4e-20 Carbohydrate binding domain CBM49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48930
fragaria_vesca FvH4_5g29090
malus_domestica MD16G1001700.v1.1
prunus_persica Prupe.1G346900_v2.0.a1
pyrus_communis pycom16g00390
rosa_chinensis RchiOBHm_Chr7g0221721 RchiOBHm_Chr7g0221731
rosa_laevigata RLG00000002135
rosa_multiflora Rmu_co8400965.1_g000001 Rmu_sc0003311.1_g000002
rosa_roxburghii Rroxscaffold_3G00238090
rosa_rugosa Rorug07G0203000
rosa_samantha Rh7AG343900 Rh7BG334300 Rh7CG361300 Rh7DG341700
rosa_wichuraiana Rw0G003250 Rw7G029160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1512
AatII GACGTC 1 cut(s) 1055
Acc65I GGTACC 1 cut(s) 1326
AccB1I GGYRCC 5 cut(s) 1326, 1364, 1448, 1520, 1556
AccB7I CCANNNNNTGG 2 cut(s) 375, 1672
AccBSI CCGCTC 1 cut(s) 1413
AccI GTMKAC 1 cut(s) 1077
AccII CGCG 2 cut(s) 83, 183
AclI AACGTT 1 cut(s) 1863
AclWI GGATC 2 cut(s) 459, 1747
AcoI YGGCCR 2 cut(s) 567, 1126
AcsI RAATTY 5 cut(s) 57, 263, 330, 694, 836
AcuI CTGAAG 1 cut(s) 1700
AcyI GRCGYC 1 cut(s) 1052
AdeI CACNNNGTG 1 cut(s) 1672
AfaI GTAC 6 cut(s) 773, 1019, 1046, 1328, 1587, 1677
AfiI CCNNNNNNNGG 5 cut(s) 375, 1294, 1463, 1553, 1672
AgeI ACCGGT 1 cut(s) 1451
AgsI TTSAA 5 cut(s) 317, 583, 1266, 1492, 1727
AjiI CACGTC 1 cut(s) 1323
AjnI CCWGG 2 cut(s) 108, 1813
Alw21I GWGCWC 1 cut(s) 1149
Alw26I GTCTC 4 cut(s) 974, 1313, 1592, 1757
AlwI GGATC 2 cut(s) 459, 1747
Ama87I CYCGRG 3 cut(s) 190, 1005, 1337
AoxI GGCC 8 cut(s) 105, 205, 366, 567, 939, 1126, 1378, 1465
ApeKI GCWGC 2 cut(s) 551, 1810
ApoI RAATTY 5 cut(s) 57, 263, 330, 694, 836
AsiGI ACCGGT 1 cut(s) 1451
Asp718I GGTACC 1 cut(s) 1326
AspLEI GCGC 4 cut(s) 183, 346, 1262, 1335
AspS9I GGNCC 5 cut(s) 106, 366, 532, 1379, 1454
AsuC2I CCSGG 1 cut(s) 525
AsuHPI GGTGA 3 cut(s) 85, 232, 263
AsuII TTCGAA 1 cut(s) 753
AsuNHI GCTAGC 1 cut(s) 37
AvaI CYCGRG 3 cut(s) 190, 1005, 1337
AvaII GGWCC 2 cut(s) 532, 1454
BaeGI GKGCMC 2 cut(s) 383, 1369
BanI GGYRCC 5 cut(s) 1326, 1364, 1448, 1520, 1556
BanII GRGCYC 1 cut(s) 1149
BbsI GAAGAC 1 cut(s) 483
Bbv12I GWGCWC 1 cut(s) 1149
BbvI GCAGC 2 cut(s) 563, 1822
BccI CCATC 9 cut(s) 271, 369, 377, 434, 528, 816, 977, 1509, 1877
BceAI ACGGC 5 cut(s) 115, 220, 785, 1058, 1141
BciT130I CCWGG 2 cut(s) 110, 1815
BclI TGATCA 1 cut(s) 253
BcnI CCSGG 1 cut(s) 525
BcoDI GTCTC 4 cut(s) 974, 1313, 1592, 1757
BfaI CTAG 3 cut(s) 38, 1070, 1416
BfmI CTRYAG 1 cut(s) 549
BfoI RGCGCY 2 cut(s) 1263, 1336
BglI GCCNNNNNGGC 1 cut(s) 560
BglII AGATCT 1 cut(s) 1729
BisI GCNGC 8 cut(s) 184, 552, 555, 570, 738, 741, 1098, 1811
BlsI GCNGC 8 cut(s) 185, 553, 556, 571, 739, 742, 1099, 1812
BmcAI AGTACT 1 cut(s) 773
Bme1390I CCNGG 3 cut(s) 110, 525, 1815
Bme18I GGWCC 2 cut(s) 532, 1454
BmeT110I CYCGRG 3 cut(s) 190, 1005, 1337
BmgBI CACGTC 1 cut(s) 1323
BmgT120I GGNCC 5 cut(s) 106, 366, 532, 1379, 1454
BmiI GGNNCC 6 cut(s) 1328, 1366, 1450, 1456, 1522, 1558
BmrFI CCNGG 3 cut(s) 110, 525, 1815
BmrI ACTGGG 1 cut(s) 359
BmsI GCATC 3 cut(s) 235, 572, 1506
BmtI GCTAGC 1 cut(s) 41
BmuI ACTGGG 1 cut(s) 359
BpiI GAAGAC 1 cut(s) 483
BpmI CTGGAG 3 cut(s) 956, 1578, 1739
Bpu14I TTCGAA 1 cut(s) 753
BpuEI CTTGAG 3 cut(s) 182, 198, 764
BpuMI CCSGG 1 cut(s) 525
BsaAI YACGTR 2 cut(s) 1021, 1048
BsaHI GRCGYC 1 cut(s) 1052
BsaI GGTCTC 2 cut(s) 1592, 1757
BsaJI CCNNGG 4 cut(s) 108, 558, 1338, 1843
BsaWI WCCGGW 1 cut(s) 1451
BsaXI ACNNNNNCTCC 2 cut(s) 1385, 1415
Bsc4I CCNNNNNNNGG 5 cut(s) 375, 1294, 1463, 1553, 1672
Bse118I RCCGGY 1 cut(s) 1451
Bse1I ACTGG 5 cut(s) 182, 354, 823, 1238, 1583
BseBI CCWGG 2 cut(s) 110, 1815
BseDI CCNNGG 4 cut(s) 108, 558, 1338, 1843
BseGI GGATG 9 cut(s) 369, 403, 520, 727, 827, 1497, 1501, 1714, 1755
BseLI CCNNNNNNNGG 5 cut(s) 375, 1294, 1463, 1553, 1672
BseMII CTCAG 3 cut(s) 179, 1812, 1868
BseNI ACTGG 5 cut(s) 182, 354, 823, 1238, 1583
BseRI GAGGAG 2 cut(s) 602, 1139
BseSI GKGCMC 2 cut(s) 383, 1369
BseX3I CGGCCG 1 cut(s) 567
BseXI GCAGC 2 cut(s) 563, 1822
BseYI CCCAGC 2 cut(s) 462, 1533
BsgI GTGCAG 1 cut(s) 492
Bsh1236I CGCG 2 cut(s) 83, 183
Bsh1285I CGRYCG 3 cut(s) 570, 1299, 1411
BshFI GGCC 8 cut(s) 107, 207, 368, 569, 941, 1128, 1380, 1467
BshNI GGYRCC 5 cut(s) 1326, 1364, 1448, 1520, 1556
BshTI ACCGGT 1 cut(s) 1451
BsiEI CGRYCG 3 cut(s) 570, 1299, 1411
BsiHKAI GWGCWC 1 cut(s) 1149
BsiHKCI CYCGRG 3 cut(s) 190, 1005, 1337
BsiSI CCGG 5 cut(s) 158, 525, 540, 1446, 1452
BslFI GGGAC 5 cut(s) 207, 441, 458, 1104, 1440
BslI CCNNNNNNNGG 5 cut(s) 375, 1294, 1463, 1553, 1672
BsmAI GTCTC 4 cut(s) 974, 1313, 1592, 1757
BsmBI CGTCTC 1 cut(s) 1313
BsmFI GGGAC 5 cut(s) 207, 441, 458, 1104, 1440
BsnI GGCC 8 cut(s) 107, 207, 368, 569, 941, 1128, 1380, 1467
Bso31I GGTCTC 2 cut(s) 1592, 1757
BsoBI CYCGRG 3 cut(s) 190, 1005, 1337
Bsp119I TTCGAA 1 cut(s) 753
Bsp1286I GDGCHC 3 cut(s) 383, 1149, 1369
Bsp143I GATC 5 cut(s) 253, 451, 766, 1729, 1739
BspANI GGCC 8 cut(s) 107, 207, 368, 569, 941, 1128, 1380, 1467
BspCNI CTCAG 3 cut(s) 178, 1813, 1867
BspFNI CGCG 2 cut(s) 83, 183
BspHI TCATGA 1 cut(s) 393
BspLI GGNNCC 6 cut(s) 1328, 1366, 1450, 1456, 1522, 1558
BspMAI CTGCAG 1 cut(s) 553
BspOI GCTAGC 1 cut(s) 41
BspPI GGATC 2 cut(s) 459, 1747
BspQI GCTCTTC 1 cut(s) 641
BspT104I TTCGAA 1 cut(s) 753
BspT107I GGYRCC 5 cut(s) 1326, 1364, 1448, 1520, 1556
BspTNI GGTCTC 2 cut(s) 1592, 1757
BsrBI CCGCTC 1 cut(s) 1413
BsrFI RCCGGY 1 cut(s) 1451
BsrI ACTGG 5 cut(s) 182, 354, 823, 1238, 1583
BssAI RCCGGY 1 cut(s) 1451
BssECI CCNNGG 4 cut(s) 108, 558, 1338, 1843
BssMI GATC 5 cut(s) 253, 451, 766, 1729, 1739
BssNI GRCGYC 1 cut(s) 1052
BssT1I CCWWGG 2 cut(s) 558, 1843
Bst2UI CCWGG 2 cut(s) 110, 1815
Bst4CI ACNGT 4 cut(s) 27, 289, 1364, 1642
Bst6I CTCTTC 3 cut(s) 601, 641, 895
BstACI GRCGYC 1 cut(s) 1052
BstBAI YACGTR 2 cut(s) 1021, 1048
BstBI TTCGAA 1 cut(s) 753
BstC8I GCNNGC 2 cut(s) 39, 471
BstDEI CTNAG 6 cut(s) 165, 616, 690, 1476, 1821, 1854
BstF5I GGATG 9 cut(s) 369, 403, 520, 727, 827, 1497, 1501, 1714, 1755
BstFNI CGCG 2 cut(s) 83, 183
BstH2I RGCGCY 2 cut(s) 1263, 1336
BstHHI GCGC 4 cut(s) 183, 346, 1262, 1335
BstKTI GATC 5 cut(s) 256, 454, 769, 1732, 1742
BstMAI GTCTC 4 cut(s) 974, 1313, 1592, 1757
BstMBI GATC 5 cut(s) 253, 451, 766, 1729, 1739
BstMCI CGRYCG 3 cut(s) 570, 1299, 1411
BstMWI GCNNNNNNNGC 6 cut(s) 43, 560, 569, 575, 1103, 1824
BstNI CCWGG 2 cut(s) 110, 1815
BstNSI RCATGY 1 cut(s) 963
BstSCI CCNGG 3 cut(s) 108, 523, 1813
BstSFI CTRYAG 1 cut(s) 549
BstSLI GKGCMC 2 cut(s) 383, 1369
BstUI CGCG 2 cut(s) 83, 183
BstV1I GCAGC 2 cut(s) 563, 1822
BstV2I GAAGAC 1 cut(s) 483
BstX2I RGATCY 2 cut(s) 1729, 1739
BstYI RGATCY 2 cut(s) 1729, 1739
BstZI CGGCCG 1 cut(s) 567
BsuRI GGCC 8 cut(s) 107, 207, 368, 569, 941, 1128, 1380, 1467
BtgZI GCGATG 1 cut(s) 1674
BtrI CACGTC 1 cut(s) 1323
BtsCI GGATG 9 cut(s) 369, 403, 520, 727, 827, 1497, 1501, 1714, 1755
BtsI GCAGTG 1 cut(s) 684
BtsIMutI CAGTG 3 cut(s) 294, 381, 684
Cac8I GCNNGC 2 cut(s) 39, 471
CciI TCATGA 1 cut(s) 393
CfoI GCGC 4 cut(s) 183, 346, 1262, 1335
Cfr10I RCCGGY 1 cut(s) 1451
Cfr13I GGNCC 5 cut(s) 106, 366, 532, 1379, 1454
Csp6I GTAC 6 cut(s) 772, 1018, 1045, 1327, 1586, 1676
CspAI ACCGGT 1 cut(s) 1451
CviQI GTAC 6 cut(s) 772, 1018, 1045, 1327, 1586, 1676
DdeI CTNAG 6 cut(s) 165, 616, 690, 1476, 1821, 1854
DpnI GATC 5 cut(s) 255, 453, 768, 1731, 1741
DpnII GATC 5 cut(s) 253, 451, 766, 1729, 1739
DraIII CACNNNGTG 1 cut(s) 1672
EaeI YGGCCR 2 cut(s) 567, 1126
EagI CGGCCG 1 cut(s) 567
Eam1104I CTCTTC 3 cut(s) 601, 641, 895
EarI CTCTTC 3 cut(s) 601, 641, 895
EciI GGCGGA 3 cut(s) 828, 1084, 1187
Ecl136II GAGCTC 1 cut(s) 1147
EclXI CGGCCG 1 cut(s) 567
Eco130I CCWWGG 2 cut(s) 558, 1843
Eco24I GRGCYC 1 cut(s) 1149
Eco31I GGTCTC 2 cut(s) 1592, 1757
Eco32I GATATC 1 cut(s) 1704
Eco47I GGWCC 2 cut(s) 532, 1454
Eco52I CGGCCG 1 cut(s) 567
Eco53kI GAGCTC 1 cut(s) 1147
Eco57I CTGAAG 1 cut(s) 1700
Eco88I CYCGRG 3 cut(s) 190, 1005, 1337
EcoICRI GAGCTC 1 cut(s) 1147
EcoO109I RGGNCCY 2 cut(s) 106, 1379
EcoRI GAATTC 2 cut(s) 57, 836
EcoRII CCWGG 2 cut(s) 108, 1813
EcoRV GATATC 1 cut(s) 1704
EcoT14I CCWWGG 2 cut(s) 558, 1843
EcoT38I GRGCYC 1 cut(s) 1149
ErhI CCWWGG 2 cut(s) 558, 1843
Esp3I CGTCTC 1 cut(s) 1313
FalI AAGNNNNNCTT 2 cut(s) 484, 516
FaqI GGGAC 5 cut(s) 207, 441, 458, 1104, 1440
FbaI TGATCA 1 cut(s) 253
FblI GTMKAC 1 cut(s) 1077
Fnu4HI GCNGC 8 cut(s) 184, 552, 555, 570, 738, 741, 1098, 1811
FokI GGATG 9 cut(s) 356, 390, 507, 734, 834, 1484, 1488, 1721, 1742
FriOI GRGCYC 1 cut(s) 1149
Fsp4HI GCNGC 8 cut(s) 184, 552, 555, 570, 738, 741, 1098, 1811
FspBI CTAG 3 cut(s) 38, 1070, 1416
GlaI GCGC 4 cut(s) 182, 345, 1261, 1334
GluI GCNGC 8 cut(s) 184, 552, 555, 570, 738, 741, 1098, 1811
GsaI CCCAGC 2 cut(s) 466, 1537
GsuI CTGGAG 3 cut(s) 956, 1578, 1739
HaeII RGCGCY 2 cut(s) 1263, 1336
HaeIII GGCC 8 cut(s) 107, 207, 368, 569, 941, 1128, 1380, 1467
HapII CCGG 5 cut(s) 158, 525, 540, 1446, 1452
HhaI GCGC 4 cut(s) 183, 346, 1262, 1335
Hin1I GRCGYC 1 cut(s) 1052
Hin6I GCGC 4 cut(s) 181, 344, 1260, 1333
HinP1I GCGC 4 cut(s) 181, 344, 1260, 1333
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 7 cut(s) 71, 188, 195, 446, 654, 674, 1615
HpaII CCGG 5 cut(s) 158, 525, 540, 1446, 1452
HphI GGTGA 3 cut(s) 85, 232, 263
Hpy166II GTNNAC 6 cut(s) 175, 223, 285, 793, 1078, 1249
Hpy188III TCNNGA 6 cut(s) 192, 394, 973, 997, 1121, 1595
Hpy8I GTNNAC 6 cut(s) 175, 223, 285, 793, 1078, 1249
Hpy99I CGWCG 3 cut(s) 763, 1127, 1409
HpyAV CCTTC 2 cut(s) 884, 1547
HpyCH4III ACNGT 4 cut(s) 27, 289, 1364, 1642
HpyCH4IV ACGT 7 cut(s) 1020, 1047, 1052, 1322, 1353, 1839, 1863
HpyF10VI GCNNNNNNNGC 6 cut(s) 43, 560, 569, 575, 1103, 1824
HpyF3I CTNAG 6 cut(s) 165, 616, 690, 1476, 1821, 1854
HpySE526I ACGT 7 cut(s) 1020, 1047, 1052, 1322, 1353, 1839, 1863
Hsp92I GRCGYC 1 cut(s) 1052
HspAI GCGC 4 cut(s) 181, 344, 1260, 1333
KpnI GGTACC 1 cut(s) 1330
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 5 cut(s) 253, 451, 766, 1729, 1739
LguI GCTCTTC 1 cut(s) 641
LmnI GCTCC 4 cut(s) 308, 466, 1152, 1597
Lsp1109I GCAGC 2 cut(s) 563, 1822
LweI GCATC 3 cut(s) 235, 572, 1506
MaeI CTAG 3 cut(s) 38, 1070, 1416
MaeII ACGT 7 cut(s) 1020, 1047, 1052, 1322, 1353, 1839, 1863
MaeIII GTNAC 3 cut(s) 289, 1048, 1164
MalI GATC 5 cut(s) 255, 453, 768, 1731, 1741
MbiI CCGCTC 1 cut(s) 1413
MboI GATC 5 cut(s) 253, 451, 766, 1729, 1739
MboII GAAGA 7 cut(s) 488, 588, 628, 912, 1583, 1693, 1739
MflI RGATCY 2 cut(s) 1729, 1739
MhlI GDGCHC 3 cut(s) 383, 1149, 1369
MluCI AATT 7 cut(s) 57, 263, 330, 694, 836, 1198, 1231
MlyI GAGTC 4 cut(s) 182, 189, 440, 683
MmeI TCCRAC 2 cut(s) 873, 1428
MseI TTAA 1 cut(s) 1885
MspI CCGG 5 cut(s) 158, 525, 540, 1446, 1452
MspR9I CCNGG 3 cut(s) 110, 525, 1815
MvaI CCWGG 2 cut(s) 110, 1815
MvnI CGCG 2 cut(s) 83, 183
MwoI GCNNNNNNNGC 6 cut(s) 43, 560, 569, 575, 1103, 1824
NciI CCSGG 1 cut(s) 525
NdeII GATC 5 cut(s) 253, 451, 766, 1729, 1739
NheI GCTAGC 1 cut(s) 37
NlaIV GGNNCC 6 cut(s) 1328, 1366, 1450, 1456, 1522, 1558
NmeAIII GCCGAG 1 cut(s) 1164
NmuCI GTSAC 3 cut(s) 289, 1048, 1164
NspI RCATGY 1 cut(s) 963
NspV TTCGAA 1 cut(s) 753
PaeR7I CTCGAG 1 cut(s) 1005
PagI TCATGA 1 cut(s) 393
PciSI GCTCTTC 1 cut(s) 641
PcsI WCGNNNNNNNCGW 3 cut(s) 370, 1697, 1869
PfeI GAWTC 3 cut(s) 71, 654, 1615
PflMI CCANNNNNTGG 2 cut(s) 375, 1672
PinAI ACCGGT 1 cut(s) 1451
PkrI GCNGC 8 cut(s) 185, 553, 556, 571, 739, 742, 1099, 1812
PleI GAGTC 4 cut(s) 182, 189, 440, 682
PpsI GAGTC 4 cut(s) 182, 189, 440, 682
Ppu21I YACGTR 2 cut(s) 1021, 1048
PsiI TTATAA 1 cut(s) 1512
Psp124BI GAGCTC 1 cut(s) 1149
Psp1406I AACGTT 1 cut(s) 1863
Psp6I CCWGG 2 cut(s) 108, 1813
PspFI CCCAGC 2 cut(s) 462, 1533
PspGI CCWGG 2 cut(s) 108, 1813
PspN4I GGNNCC 6 cut(s) 1328, 1366, 1450, 1456, 1522, 1558
PspPI GGNCC 5 cut(s) 106, 366, 532, 1379, 1454
PspXI VCTCGAGB 1 cut(s) 1005
PsrI GAACNNNNNNTAC 2 cut(s) 755, 787
PstI CTGCAG 1 cut(s) 553
PsuI RGATCY 2 cut(s) 1729, 1739
RsaI GTAC 6 cut(s) 773, 1019, 1046, 1328, 1587, 1677
RsaNI GTAC 6 cut(s) 772, 1018, 1045, 1327, 1586, 1676
SacI GAGCTC 1 cut(s) 1149
SapI GCTCTTC 1 cut(s) 641
SaqAI TTAA 1 cut(s) 1885
SatI GCNGC 8 cut(s) 184, 552, 555, 570, 738, 741, 1098, 1811
Sau3AI GATC 5 cut(s) 253, 451, 766, 1729, 1739
Sau96I GGNCC 5 cut(s) 106, 366, 532, 1379, 1454
ScaI AGTACT 1 cut(s) 773
SchI GAGTC 4 cut(s) 182, 189, 440, 683
ScrFI CCNGG 3 cut(s) 110, 525, 1815
SduI GDGCHC 3 cut(s) 383, 1149, 1369
SfaNI GCATC 3 cut(s) 235, 572, 1506
SfcI CTRYAG 1 cut(s) 549
Sfr274I CTCGAG 1 cut(s) 1005
SfuI TTCGAA 1 cut(s) 753
SinI GGWCC 2 cut(s) 532, 1454
SlaI CTCGAG 1 cut(s) 1005
SmlI CTYRAG 4 cut(s) 197, 213, 779, 1005
SmoI CTYRAG 4 cut(s) 197, 213, 779, 1005
Sse9I AATT 7 cut(s) 57, 263, 330, 694, 836, 1198, 1231
SspMI CTAG 3 cut(s) 38, 1070, 1416
SstI GAGCTC 1 cut(s) 1149
StyD4I CCNGG 3 cut(s) 108, 523, 1813
StyI CCWWGG 2 cut(s) 558, 1843
TaaI ACNGT 4 cut(s) 27, 289, 1364, 1642
TaiI ACGT 7 cut(s) 1023, 1050, 1055, 1325, 1356, 1842, 1866
TaqI TCGA 5 cut(s) 69, 132, 753, 1006, 1299
TaqII GACCGA 1 cut(s) 1445
TasI AATT 7 cut(s) 57, 263, 330, 694, 836, 1198, 1231
TatI WGTACW 2 cut(s) 771, 1585
TauI GCSGC 6 cut(s) 186, 557, 572, 740, 743, 1100
TfiI GAWTC 3 cut(s) 71, 654, 1615
Tru1I TTAA 1 cut(s) 1885
Tru9I TTAA 1 cut(s) 1885
TscAI CASTG 3 cut(s) 294, 388, 691
TseFI GTSAC 3 cut(s) 289, 1048, 1164
TseI GCWGC 2 cut(s) 551, 1810
Tsp45I GTSAC 3 cut(s) 289, 1048, 1164
TspDTI ATGAA 9 cut(s) 291, 382, 410, 611, 696, 738, 1437, 1584, 1822
TspRI CASTG 3 cut(s) 294, 388, 691
Van91I CCANNNNNTGG 2 cut(s) 375, 1672
VpaK11BI GGWCC 2 cut(s) 532, 1454
XapI RAATTY 5 cut(s) 57, 263, 330, 694, 836
XceI RCATGY 1 cut(s) 963
XcmI CCANNNNNNNNNTGG 1 cut(s) 980
XhoI CTCGAG 1 cut(s) 1005
XmiI GTMKAC 1 cut(s) 1077
XspI CTAG 3 cut(s) 38, 1070, 1416
ZraI GACGTC 1 cut(s) 1053
ZrmI AGTACT 1 cut(s) 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.