Rmu_co8400965.1_g000001

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8400965.1
OG0013278
Physical Location & Seq
Reverse (-)
2 .. 1236
1235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8400965.1_g000001.1.cds

Sequence Viewer

Length: 870 bp
atggttattattttgatacttaaggacgagctactatgggctgcggcatggctctacgaagcaaccaacgatgactactacctcaagtatgctatggacaacgccgtgtatatgggtggaactggatggtcagtcaaagagttttcttgggataacaaatatgctggtgtccaaatccttctcacaaaggttttgttagagggaagaggtggtgcatatacttccacactgaaacaatatcaggccaaagcagattactttgcatgtgcttgtctgcagaaaaatgatggctacaatgtccatacaactcctgggggcttattatacgtgcgtgaatggaacaacttgcagtatgttacttctgcttcgtttcttctagctgtttactccgattatctctctgctgcaaatgccaagctcaattgtccggaaggacaaattcaacctcaagaggtcctcgactttgcaaagtcacaggttgactacattcttggtaaaaaccccaagtcaatgagctacatagttgggtatggagccaagtatccagttcatgtgcaccacagagatgcttccattccttctatatcagttcttcatgctgtggttggatgtgtgcaaggatttgagaaatggtatcaccgcactgagggaaatcctaatgtcgtctatggggcacttgtgggaggtcctgaccagaatgacaacttctccgatgaccgctccaactatgaagggacagagcctacaatttctggcactggtcccctagtaggcctcttctctaagctacagagtttaaatggtggtttcaatagaggatcaccaaaatcccatacacccacaccaagtgcctatcaccctgaaacaaca
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
CBM49 Glyco_hydro_9

Protein Analysis

290

Amino Acids

32.03

Weight (kDa)

5.89

Isoelectric Point (pI)

28.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48930
fragaria_vesca FvH4_5g29090
malus_domestica MD16G1001700.v1.1
prunus_persica Prupe.1G346900_v2.0.a1
pyrus_communis pycom16g00390
rosa_chinensis RchiOBHm_Chr7g0221721 RchiOBHm_Chr7g0221731
rosa_laevigata RLG00000002135
rosa_multiflora Rmu_co8400965.1_g000001 Rmu_sc0003311.1_g000002
rosa_roxburghii Rroxscaffold_3G00238090
rosa_rugosa Rorug07G0203000
rosa_samantha Rh7AG343900 Rh7BG334300 Rh7CG361300 Rh7DG341700
rosa_wichuraiana Rw0G003250 Rw7G029160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 720
AccIII TCCGGA 1 cut(s) 427
AciI CCGC 3 cut(s) 44, 640, 718
AclWI GGATC 1 cut(s) 826
AcsI RAATTY 1 cut(s) 438
AdeI CACNNNGTG 1 cut(s) 848
AfiI CCNNNNNNNGG 2 cut(s) 646, 770
AflII CTTAAG 1 cut(s) 20
AgsI TTSAA 2 cut(s) 443, 811
AjnI CCWGG 1 cut(s) 310
AluBI AGCT 5 cut(s) 31, 380, 418, 516, 787
AluI AGCT 5 cut(s) 31, 380, 418, 516, 787
Alw21I GWGCWC 1 cut(s) 558
Alw44I GTGCAC 1 cut(s) 554
AlwI GGATC 1 cut(s) 826
Aor13HI TCCGGA 1 cut(s) 427
AoxI GGCC 2 cut(s) 243, 772
ApaLI GTGCAC 1 cut(s) 554
ApeKI GCWGC 2 cut(s) 41, 404
ApoI RAATTY 1 cut(s) 438
AspS9I GGNCC 3 cut(s) 454, 686, 761
AsuHPI GGTGA 3 cut(s) 629, 813, 848
AvaII GGWCC 3 cut(s) 454, 686, 761
BaeGI GKGCMC 2 cut(s) 558, 676
Bbv12I GWGCWC 1 cut(s) 558
BbvI GCAGC 2 cut(s) 28, 391
BccI CCATC 2 cut(s) 120, 281
BceAI ACGGC 1 cut(s) 89
BciT130I CCWGG 1 cut(s) 312
BciVI GTATCC 1 cut(s) 552
BfaI CTAG 2 cut(s) 377, 767
BfmI CTRYAG 2 cut(s) 275, 788
BfrI CTTAAG 1 cut(s) 20
BfuI GTATCC 1 cut(s) 552
BisI GCNGC 3 cut(s) 42, 45, 405
BlsI GCNGC 3 cut(s) 43, 46, 406
Bme1390I CCNGG 1 cut(s) 312
Bme18I GGWCC 3 cut(s) 454, 686, 761
BmgT120I GGNCC 3 cut(s) 454, 686, 761
BmiI GGNNCC 2 cut(s) 535, 763
BmrFI CCNGG 1 cut(s) 312
BmsI GCATC 1 cut(s) 556
BpuEI CTTGAG 2 cut(s) 68, 432
BsaAI YACGTR 1 cut(s) 328
BsaJI CCNNGG 1 cut(s) 311
BsaWI WCCGGW 1 cut(s) 427
BsaXI ACNNNNNCTCC 2 cut(s) 692, 722
Bsc4I CCNNNNNNNGG 2 cut(s) 646, 770
Bse1I ACTGG 3 cut(s) 127, 545, 763
BseAI TCCGGA 1 cut(s) 427
BseBI CCWGG 1 cut(s) 312
BseDI CCNNGG 1 cut(s) 311
BseGI GGATG 2 cut(s) 131, 614
BseLI CCNNNNNNNGG 2 cut(s) 646, 770
BseMII CTCAG 1 cut(s) 636
BseNI ACTGG 3 cut(s) 127, 545, 763
BseSI GKGCMC 2 cut(s) 558, 676
BseXI GCAGC 2 cut(s) 28, 391
BshFI GGCC 2 cut(s) 245, 774
BsiHKAI GWGCWC 1 cut(s) 558
BsiSI CCGG 1 cut(s) 428
BslFI GGGAC 2 cut(s) 747, 748
BslI CCNNNNNNNGG 2 cut(s) 646, 770
BsmFI GGGAC 2 cut(s) 747, 748
BsnI GGCC 2 cut(s) 245, 774
Bsp1286I GDGCHC 2 cut(s) 558, 676
Bsp13I TCCGGA 1 cut(s) 427
Bsp143I GATC 1 cut(s) 818
BspACI CCGC 3 cut(s) 44, 640, 718
BspANI GGCC 2 cut(s) 245, 774
BspCNI CTCAG 1 cut(s) 637
BspEI TCCGGA 1 cut(s) 427
BspLI GGNNCC 2 cut(s) 535, 763
BspMAI CTGCAG 1 cut(s) 279
BspPI GGATC 1 cut(s) 826
BspTI CTTAAG 1 cut(s) 20
BsrBI CCGCTC 1 cut(s) 720
BsrI ACTGG 3 cut(s) 127, 545, 763
BssECI CCNNGG 1 cut(s) 311
BssMI GATC 1 cut(s) 818
Bst2UI CCWGG 1 cut(s) 312
Bst6I CTCTTC 2 cut(s) 199, 782
BstAFI CTTAAG 1 cut(s) 20
BstBAI YACGTR 1 cut(s) 328
BstDEI CTNAG 2 cut(s) 645, 783
BstF5I GGATG 2 cut(s) 131, 614
BstKTI GATC 1 cut(s) 821
BstMBI GATC 1 cut(s) 818
BstMWI GCNNNNNNNGC 1 cut(s) 410
BstNI CCWGG 1 cut(s) 312
BstNSI RCATGY 1 cut(s) 267
BstSCI CCNGG 1 cut(s) 310
BstSFI CTRYAG 2 cut(s) 275, 788
BstSLI GKGCMC 2 cut(s) 558, 676
BstV1I GCAGC 2 cut(s) 28, 391
BsuI GTATCC 1 cut(s) 552
BsuRI GGCC 2 cut(s) 245, 774
BtsCI GGATG 2 cut(s) 131, 614
BtsIMutI CAGTG 3 cut(s) 227, 642, 756
Cfr13I GGNCC 3 cut(s) 454, 686, 761
CviAII CATG 4 cut(s) 48, 264, 551, 596
DdeI CTNAG 2 cut(s) 645, 783
DpnI GATC 1 cut(s) 820
DpnII GATC 1 cut(s) 818
DraI TTTAAA 1 cut(s) 798
DraIII CACNNNGTG 1 cut(s) 848
Eam1104I CTCTTC 2 cut(s) 199, 782
EarI CTCTTC 2 cut(s) 199, 782
Eco147I AGGCCT 1 cut(s) 774
Eco47I GGWCC 3 cut(s) 454, 686, 761
EcoO109I RGGNCCY 2 cut(s) 454, 686
EcoRII CCWGG 1 cut(s) 310
FaeI CATG 4 cut(s) 51, 267, 554, 599
FaqI GGGAC 2 cut(s) 747, 748
FatI CATG 4 cut(s) 47, 263, 550, 595
Fnu4HI GCNGC 3 cut(s) 42, 45, 405
FokI GGATG 2 cut(s) 138, 621
Fsp4HI GCNGC 3 cut(s) 42, 45, 405
FspBI CTAG 2 cut(s) 377, 767
GluI GCNGC 3 cut(s) 42, 45, 405
HaeIII GGCC 2 cut(s) 245, 774
HapII CCGG 1 cut(s) 428
Hin1II CATG 4 cut(s) 51, 267, 554, 599
HincII GTYRAC 1 cut(s) 481
HindII GTYRAC 1 cut(s) 481
HpaII CCGG 1 cut(s) 428
HphI GGTGA 3 cut(s) 629, 813, 848
Hpy166II GTNNAC 3 cut(s) 385, 481, 556
Hpy188I TCNGA 2 cut(s) 391, 712
Hpy188III TCNNGA 3 cut(s) 428, 449, 689
Hpy8I GTNNAC 3 cut(s) 385, 481, 556
HpyAV CCTTC 4 cut(s) 188, 425, 588, 725
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 8 cut(s) 215, 263, 277, 349, 407, 467, 556, 616
HpyF10VI GCNNNNNNNGC 1 cut(s) 410
HpyF3I CTNAG 2 cut(s) 645, 783
HpySE526I ACGT 1 cut(s) 327
Hsp92II CATG 4 cut(s) 51, 267, 554, 599
Kpn2I TCCGGA 1 cut(s) 427
Kzo9I GATC 1 cut(s) 818
LmnI GCTCC 2 cut(s) 533, 725
Lsp1109I GCAGC 2 cut(s) 28, 391
LweI GCATC 1 cut(s) 556
MaeI CTAG 2 cut(s) 377, 767
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 2 cut(s) 355, 471
MalI GATC 1 cut(s) 820
MbiI CCGCTC 1 cut(s) 720
MboI GATC 1 cut(s) 818
MboII GAAGA 4 cut(s) 216, 365, 584, 769
MfeI CAATTG 1 cut(s) 421
MhlI GDGCHC 2 cut(s) 558, 676
MluCI AATT 3 cut(s) 421, 438, 747
MmeI TCCRAC 2 cut(s) 586, 747
MroI TCCGGA 1 cut(s) 427
MseI TTAA 2 cut(s) 21, 797
MslI CAYNNNNRTG 1 cut(s) 564
MspCI CTTAAG 1 cut(s) 20
MspI CCGG 1 cut(s) 428
MspR9I CCNGG 1 cut(s) 312
MunI CAATTG 1 cut(s) 421
MvaI CCWGG 1 cut(s) 312
MwoI GCNNNNNNNGC 1 cut(s) 410
NdeII GATC 1 cut(s) 818
NlaIII CATG 4 cut(s) 51, 267, 554, 599
NlaIV GGNNCC 2 cut(s) 535, 763
NmuCI GTSAC 1 cut(s) 471
NspI RCATGY 1 cut(s) 267
PceI AGGCCT 1 cut(s) 774
PkrI GCNGC 3 cut(s) 43, 46, 406
Ppu21I YACGTR 1 cut(s) 328
PpuMI RGGWCCY 2 cut(s) 454, 686
Psp5II RGGWCCY 2 cut(s) 454, 686
Psp6I CCWGG 1 cut(s) 310
PspGI CCWGG 1 cut(s) 310
PspN4I GGNNCC 2 cut(s) 535, 763
PspPI GGNCC 3 cut(s) 454, 686, 761
PspPPI RGGWCCY 2 cut(s) 454, 686
PstI CTGCAG 1 cut(s) 279
RseI CAYNNNNRTG 1 cut(s) 564
SaqAI TTAA 2 cut(s) 21, 797
SatI GCNGC 3 cut(s) 42, 45, 405
Sau3AI GATC 1 cut(s) 818
Sau96I GGNCC 3 cut(s) 454, 686, 761
ScrFI CCNGG 1 cut(s) 312
SduI GDGCHC 2 cut(s) 558, 676
SfaNI GCATC 1 cut(s) 556
SfcI CTRYAG 2 cut(s) 275, 788
SinI GGWCC 3 cut(s) 454, 686, 761
SmiMI CAYNNNNRTG 1 cut(s) 564
SmlI CTYRAG 3 cut(s) 20, 83, 447
SmoI CTYRAG 3 cut(s) 20, 83, 447
Sse9I AATT 3 cut(s) 421, 438, 747
SseBI AGGCCT 1 cut(s) 774
SsiI CCGC 3 cut(s) 44, 640, 718
SspMI CTAG 2 cut(s) 377, 767
StuI AGGCCT 1 cut(s) 774
StyD4I CCNGG 1 cut(s) 310
TaiI ACGT 1 cut(s) 330
TaqI TCGA 1 cut(s) 459
TasI AATT 3 cut(s) 421, 438, 747
TauI GCSGC 1 cut(s) 47
Tru1I TTAA 2 cut(s) 21, 797
Tru9I TTAA 2 cut(s) 21, 797
TscAI CASTG 3 cut(s) 234, 649, 763
TseFI GTSAC 1 cut(s) 471
TseI GCWGC 2 cut(s) 41, 404
Tsp45I GTSAC 1 cut(s) 471
TspDTI ATGAA 3 cut(s) 539, 584, 744
TspRI CASTG 3 cut(s) 234, 649, 763
Vha464I CTTAAG 1 cut(s) 20
VneI GTGCAC 1 cut(s) 554
VpaK11BI GGWCC 3 cut(s) 454, 686, 761
XapI RAATTY 1 cut(s) 438
XceI RCATGY 1 cut(s) 267
XcmI CCANNNNNNNNNTGG 1 cut(s) 308
XspI CTAG 2 cut(s) 377, 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.