Rw0G003250

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00111
OG0013278
Physical Location & Seq
Reverse (-)
16693 .. 19626
2934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G003250.1

Sequence Viewer

Length: 1794 bp
ATGATGTTCATGAGGAGCTGCACCAAAATTGCACTAGTCTCCCTAGCTTTGCTAATGCTACTTGGTGTGGAAGCAGCCACTGCTTCAGAGTTTAGCTACGGCGAGGCTTTAGACAAGAGTTTGCTCTTTTTTGAGGCACAAAGATCTGGTAAACTGCCCCCAAACCAGCGAGTCAAGTGGCGCAGTGACTCAGGACTTAAGGATGGCCTAGCTCAAGGGGTGGACCTGGTTGGAGGGTATTATGATGCAGGGGATCATGTGAAATTTGGGCTACCAATGGCATTCACAGTGACAATGCTTTCATGGGGAGCTATTGAGTTCAGGAAGGAGATCACAGATTTAGACCAATTAGGTCAGACATTATGGGCCATTAGATGGGGCACTGACTATTTCATCAAAGCACATCCACAGCCTAATGTTCTTTGGGGACAGGTAGGAGATGGGAACTCTGATCACTATTGTTGGGAGCGCGCAGAGGACATGACAACTCCAAGAAATGCTTATAAAATTGATGAAAATCATCCCGGTTCAGACATTGCTGGTGAAACTGCAGCTGCCTTGGCCTCAGCTGCCATAGCTTTCAAGCCTTACAACTCTTCCTACTCCAATCTCCTCTTAGTTCATGCAAAACAGCTTTTCTCATTTGCAGATAGATTCCGAGGATTATATGATGAATCCATTGGGGATGCTAAGGACTTCTACACCTCATCGGGTTACTACGACGAGCTACTATGGGCTGCGGCATGGCTCTACGAAGCAACCAACGATGACTACTACCTCAAGTATGTTGTGGACAACGCCGTGTATATGGGTGGAACTGGATGGTCAGTCAAAGAGTTTTCTTGGGATAACAAATATGCTGGTGTCCAAATCCTTCTCACAAAGGTTTTGTTAGAGGGAAGAGGTGGTGCATATACTTCCACACTGAAACAATATCAGGCCAAAGCAGATTACTTTGCATGTGCTTGTCTGCAGAAAAATGATGGCTACAATGTCCATACAACTCCTGGGGGCTTATTATACGTGCGTGAATGGAACAACTTGCAGTATGTTACTTCTGCTTCGTTTCTTCTAGCTGTTTACTCCGATTATCTCTCTGCTGCAAATGCCAAGCTCAATTGTCCGGAAGGACAAATTCAACCTCAAGAGGTCCTCAACTTTGCAAAGTCACAGGTTGACTACATTCTTGGTAAAAACCCCAAGTCAATGAGCTACATAGTTGGGTATGGAGCCAAGTATCCAGTTCATGTGCACCACAGAGATGCTTCCATTCCTTCTATATCAGTTCTTCATGCTGTGGTTGGATGTGTGCAAGGATTTGAGAAATGGTATCACCGCACTGAGGGAAATCCTAATGTCGTCTATGGGGCACTTGTGGGAGGTCCTGACCAGAATGACAACTTCTCCGATGACCGCTCCAACTATGAAGGGACAGAGCCTACAATTTCTGGCACTGGTCCCCTAGTAGGCCTCTTCTCTAAGCTACAGAGTTTAAATGGTGATACTCCTCCTATCAAATTCCTTCACTCTATAACCAGCACATGGACTGTTGCAAAAACAAGTTATTATCGACACAAGGTGATACTAAAGAACACATCCCAGAAACCGATAACAAATGTGAAGCTGGTGCTTGGAAACCTCCAAGGGCCATTATGGGGTCTCTCTCCAACTCCACAGAAGAACACATATGAGCTTCCTCAATGGCAGAAAGTACTGCAGCCTGGCGCCGAGTGCACTTTTGTTTACGTTCAAGGTGGCCCTCAGGCAAAGATATCTATTCAAAGCTACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
CBM49 Glyco_hydro_9

Protein Analysis

597

Amino Acids

66.28

Weight (kDa)

6.13

Isoelectric Point (pI)

29.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 33 - 491 1.2e-168 Glycosyl hydrolase family 9
CBM49 PF09478 505 - 584 1.2e-22 Carbohydrate binding domain CBM49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48930
fragaria_vesca FvH4_5g29090
malus_domestica MD16G1001700.v1.1
prunus_persica Prupe.1G346900_v2.0.a1
pyrus_communis pycom16g00390
rosa_chinensis RchiOBHm_Chr7g0221721 RchiOBHm_Chr7g0221731
rosa_laevigata RLG00000002135
rosa_multiflora Rmu_co8400965.1_g000001 Rmu_sc0003311.1_g000002
rosa_roxburghii Rroxscaffold_3G00238090
rosa_rugosa Rorug07G0203000
rosa_samantha Rh7AG343900 Rh7BG334300 Rh7CG361300 Rh7DG341700
rosa_wichuraiana Rw0G003250 Rw7G029160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 504
AccB1I GGYRCC 1 cut(s) 1724
AccB7I CCANNNNNTGG 2 cut(s) 375, 1542
AccBSI CCGCTC 1 cut(s) 1416
AccII CGCG 1 cut(s) 471
AccIII TCCGGA 1 cut(s) 1123
AciI CCGC 3 cut(s) 740, 1336, 1414
AclWI GGATC 1 cut(s) 261
AcsI RAATTY 3 cut(s) 263, 1134, 1517
AcuI CTGAAG 1 cut(s) 69
AcyI GRCGYC 1 cut(s) 1725
AdeI CACNNNGTG 1 cut(s) 1579
AfaI GTAC 1 cut(s) 1713
AfiI CCNNNNNNNGG 5 cut(s) 375, 1342, 1466, 1542, 1655
AflII CTTAAG 1 cut(s) 197
AgsI TTSAA 4 cut(s) 583, 1139, 1751, 1781
AhlI ACTAGT 1 cut(s) 34
AjnI CCWGG 3 cut(s) 225, 1006, 1720
Alw21I GWGCWC 2 cut(s) 1254, 1736
Alw26I GTCTC 2 cut(s) 43, 1664
Alw44I GTGCAC 2 cut(s) 1250, 1732
AlwI GGATC 1 cut(s) 261
AlwNI CAGNNNCTG 1 cut(s) 80
Aor13HI TCCGGA 1 cut(s) 1123
AoxI GGCC 7 cut(s) 205, 366, 561, 939, 1468, 1646, 1756
ApaLI GTGCAC 2 cut(s) 1250, 1732
ApeKI GCWGC 8 cut(s) 18, 74, 551, 554, 569, 737, 1100, 1717
ApoI RAATTY 3 cut(s) 263, 1134, 1517
AspLEI GCGC 4 cut(s) 183, 471, 473, 1727
AspS9I GGNCC 7 cut(s) 223, 366, 1150, 1382, 1457, 1646, 1757
AsuC2I CCSGG 1 cut(s) 525
AsuHPI GGTGA 4 cut(s) 554, 1325, 1511, 1591
AvaII GGWCC 4 cut(s) 223, 1150, 1382, 1457
AxyI CCTNAGG 1 cut(s) 1761
BaeGI GKGCMC 4 cut(s) 383, 1254, 1372, 1736
BanI GGYRCC 1 cut(s) 1724
Bbv12I GWGCWC 2 cut(s) 1254, 1736
BbvCI CCTCAGC 1 cut(s) 565
BbvI GCAGC 8 cut(s) 5, 86, 541, 556, 563, 724, 1087, 1729
BccI CCATC 5 cut(s) 197, 369, 434, 816, 977
BceAI ACGGC 2 cut(s) 115, 785
BciT130I CCWGG 3 cut(s) 227, 1008, 1722
BciVI GTATCC 1 cut(s) 1248
BclI TGATCA 1 cut(s) 451
BcnI CCSGG 1 cut(s) 525
BcoDI GTCTC 2 cut(s) 43, 1664
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 5 cut(s) 35, 44, 209, 1073, 1463
BfmI CTRYAG 4 cut(s) 549, 971, 1484, 1715
BfoI RGCGCY 1 cut(s) 1728
BfrI CTTAAG 1 cut(s) 197
BfuI GTATCC 1 cut(s) 1248
BglII AGATCT 1 cut(s) 143
BisI GCNGC 9 cut(s) 19, 75, 552, 555, 570, 738, 741, 1101, 1718
BlsI GCNGC 9 cut(s) 20, 76, 553, 556, 571, 739, 742, 1102, 1719
BmcAI AGTACT 1 cut(s) 1713
Bme1390I CCNGG 4 cut(s) 227, 525, 1008, 1722
Bme18I GGWCC 4 cut(s) 223, 1150, 1382, 1457
BmgT120I GGNCC 7 cut(s) 223, 366, 1150, 1382, 1457, 1646, 1757
BmiI GGNNCC 3 cut(s) 1231, 1459, 1726
BmrFI CCNGG 4 cut(s) 227, 525, 1008, 1722
BmsI GCATC 3 cut(s) 235, 676, 1252
Bpu10I CCTNAGC 2 cut(s) 565, 690
BpuEI CTTGAG 3 cut(s) 198, 764, 1128
BpuMI CCSGG 1 cut(s) 525
BsaAI YACGTR 1 cut(s) 1024
BsaBI GATNNNNATC 1 cut(s) 516
BsaHI GRCGYC 1 cut(s) 1725
BsaI GGTCTC 1 cut(s) 1664
BsaJI CCNNGG 4 cut(s) 558, 658, 1007, 1642
BsaWI WCCGGW 1 cut(s) 1123
BsaXI ACNNNNNCTCC 2 cut(s) 1388, 1418
Bsc4I CCNNNNNNNGG 5 cut(s) 375, 1342, 1466, 1542, 1655
Bse1I ACTGG 3 cut(s) 823, 1241, 1459
Bse21I CCTNAGG 1 cut(s) 1761
Bse3DI GCAATG 1 cut(s) 534
Bse8I GATNNNNATC 1 cut(s) 516
BseAI TCCGGA 1 cut(s) 1123
BseBI CCWGG 3 cut(s) 227, 1008, 1722
BseDI CCNNGG 4 cut(s) 558, 658, 1007, 1642
BseGI GGATG 7 cut(s) 208, 403, 520, 691, 827, 1310, 1595
BseJI GATNNNNATC 1 cut(s) 516
BseLI CCNNNNNNNGG 5 cut(s) 375, 1342, 1466, 1542, 1655
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 4 cut(s) 204, 579, 1332, 1775
BseNI ACTGG 3 cut(s) 823, 1241, 1459
BsePI GCGCGC 1 cut(s) 469
BseRI GAGGAG 3 cut(s) 28, 602, 1497
BseSI GKGCMC 4 cut(s) 383, 1254, 1372, 1736
BseXI GCAGC 8 cut(s) 5, 86, 541, 556, 563, 724, 1087, 1729
BsgI GTGCAG 1 cut(s) 4
Bsh1236I CGCG 1 cut(s) 471
BshFI GGCC 7 cut(s) 207, 368, 563, 941, 1470, 1648, 1758
BshNI GGYRCC 1 cut(s) 1724
BsiHKAI GWGCWC 2 cut(s) 1254, 1736
BsiSI CCGG 2 cut(s) 525, 1124
BslFI GGGAC 3 cut(s) 441, 1443, 1444
BslI CCNNNNNNNGG 5 cut(s) 375, 1342, 1466, 1542, 1655
BsmAI GTCTC 2 cut(s) 43, 1664
BsmFI GGGAC 3 cut(s) 441, 1443, 1444
BsmI GAATGC 1 cut(s) 281
BsnI GGCC 7 cut(s) 207, 368, 563, 941, 1470, 1648, 1758
Bso31I GGTCTC 1 cut(s) 1664
Bsp1286I GDGCHC 4 cut(s) 383, 1254, 1372, 1736
Bsp13I TCCGGA 1 cut(s) 1123
Bsp143I GATC 4 cut(s) 143, 253, 330, 451
BspACI CCGC 3 cut(s) 740, 1336, 1414
BspANI GGCC 7 cut(s) 207, 368, 563, 941, 1470, 1648, 1758
BspCNI CTCAG 4 cut(s) 203, 578, 1333, 1774
BspEI TCCGGA 1 cut(s) 1123
BspFNI CGCG 1 cut(s) 471
BspHI TCATGA 1 cut(s) 9
BspLI GGNNCC 3 cut(s) 1231, 1459, 1726
BspMAI CTGCAG 3 cut(s) 553, 975, 1719
BspPI GGATC 1 cut(s) 261
BspT107I GGYRCC 1 cut(s) 1724
BspTI CTTAAG 1 cut(s) 197
BspTNI GGTCTC 1 cut(s) 1664
BsrBI CCGCTC 1 cut(s) 1416
BsrDI GCAATG 1 cut(s) 534
BsrI ACTGG 3 cut(s) 823, 1241, 1459
BssECI CCNNGG 4 cut(s) 558, 658, 1007, 1642
BssHII GCGCGC 1 cut(s) 469
BssMI GATC 4 cut(s) 143, 253, 330, 451
BssNI GRCGYC 1 cut(s) 1725
BssT1I CCWWGG 2 cut(s) 558, 1642
Bst2UI CCWGG 3 cut(s) 227, 1008, 1722
Bst4CI ACNGT 2 cut(s) 289, 1549
Bst6I CTCTTC 3 cut(s) 601, 895, 1478
BstACI GRCGYC 1 cut(s) 1725
BstAFI CTTAAG 1 cut(s) 197
BstAPI GCANNNNNTGC 1 cut(s) 80
BstBAI YACGTR 1 cut(s) 1024
BstC8I GCNNGC 1 cut(s) 471
BstDEI CTNAG 7 cut(s) 190, 565, 616, 690, 1341, 1479, 1761
BstF5I GGATG 7 cut(s) 208, 403, 520, 691, 827, 1310, 1595
BstFNI CGCG 1 cut(s) 471
BstH2I RGCGCY 1 cut(s) 1728
BstHHI GCGC 4 cut(s) 183, 471, 473, 1727
BstKTI GATC 4 cut(s) 146, 256, 333, 454
BstMAI GTCTC 2 cut(s) 43, 1664
BstMBI GATC 4 cut(s) 143, 253, 330, 451
BstMWI GCNNNNNNNGC 6 cut(s) 80, 560, 569, 575, 1106, 1731
BstNI CCWGG 3 cut(s) 227, 1008, 1722
BstNSI RCATGY 1 cut(s) 963
BstSCI CCNGG 4 cut(s) 225, 523, 1006, 1720
BstSFI CTRYAG 4 cut(s) 549, 971, 1484, 1715
BstSLI GKGCMC 4 cut(s) 383, 1254, 1372, 1736
BstUI CGCG 1 cut(s) 471
BstV1I GCAGC 8 cut(s) 5, 86, 541, 556, 563, 724, 1087, 1729
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
Bsu36I CCTNAGG 1 cut(s) 1761
BsuI GTATCC 1 cut(s) 1248
BsuRI GGCC 7 cut(s) 207, 368, 563, 941, 1470, 1648, 1758
BtsCI GGATG 7 cut(s) 208, 403, 520, 691, 827, 1310, 1595
BtsI GCAGTG 2 cut(s) 78, 190
BtsIMutI CAGTG 7 cut(s) 78, 190, 294, 381, 923, 1338, 1452
Cac8I GCNNGC 1 cut(s) 471
CaiI CAGNNNCTG 1 cut(s) 80
CciI TCATGA 1 cut(s) 9
CfoI GCGC 4 cut(s) 183, 471, 473, 1727
Cfr13I GGNCC 7 cut(s) 223, 366, 1150, 1382, 1457, 1646, 1757
CsiI ACCWGGT 1 cut(s) 225
Csp6I GTAC 1 cut(s) 1712
CviQI GTAC 1 cut(s) 1712
DdeI CTNAG 7 cut(s) 190, 565, 616, 690, 1341, 1479, 1761
DinI GGCGCC 1 cut(s) 1726
DpnI GATC 4 cut(s) 145, 255, 332, 453
DpnII GATC 4 cut(s) 143, 253, 330, 451
DraI TTTAAA 1 cut(s) 1494
DraIII CACNNNGTG 1 cut(s) 1579
Eam1104I CTCTTC 3 cut(s) 601, 895, 1478
EarI CTCTTC 3 cut(s) 601, 895, 1478
Eco130I CCWWGG 2 cut(s) 558, 1642
Eco147I AGGCCT 1 cut(s) 1470
Eco31I GGTCTC 1 cut(s) 1664
Eco32I GATATC 1 cut(s) 1773
Eco47I GGWCC 4 cut(s) 223, 1150, 1382, 1457
Eco57I CTGAAG 1 cut(s) 69
Eco81I CCTNAGG 1 cut(s) 1761
EcoO109I RGGNCCY 2 cut(s) 1150, 1382
EcoRII CCWGG 3 cut(s) 225, 1006, 1720
EcoRV GATATC 1 cut(s) 1773
EcoT14I CCWWGG 2 cut(s) 558, 1642
EgeI GGCGCC 1 cut(s) 1726
EheI GGCGCC 1 cut(s) 1726
ErhI CCWWGG 2 cut(s) 558, 1642
FalI AAGNNNNNCTT 2 cut(s) 484, 516
FaqI GGGAC 3 cut(s) 441, 1443, 1444
FauNDI CATATG 1 cut(s) 1687
FbaI TGATCA 1 cut(s) 451
Fnu4HI GCNGC 9 cut(s) 19, 75, 552, 555, 570, 738, 741, 1101, 1718
FokI GGATG 7 cut(s) 215, 390, 507, 698, 834, 1317, 1582
Fsp4HI GCNGC 9 cut(s) 19, 75, 552, 555, 570, 738, 741, 1101, 1718
FspBI CTAG 5 cut(s) 35, 44, 209, 1073, 1463
GlaI GCGC 4 cut(s) 182, 470, 472, 1726
GluI GCNGC 9 cut(s) 19, 75, 552, 555, 570, 738, 741, 1101, 1718
HaeII RGCGCY 1 cut(s) 1728
HaeIII GGCC 7 cut(s) 207, 368, 563, 941, 1470, 1648, 1758
HapII CCGG 2 cut(s) 525, 1124
HhaI GCGC 4 cut(s) 183, 471, 473, 1727
Hin1I GRCGYC 1 cut(s) 1725
Hin6I GCGC 4 cut(s) 181, 469, 471, 1725
HinP1I GCGC 4 cut(s) 181, 469, 471, 1725
HincII GTYRAC 1 cut(s) 1177
HindII GTYRAC 1 cut(s) 1177
HinfI GANTC 4 cut(s) 171, 188, 654, 674
HpaII CCGG 2 cut(s) 525, 1124
HphI GGTGA 4 cut(s) 554, 1325, 1511, 1591
Hpy166II GTNNAC 8 cut(s) 152, 223, 793, 1081, 1177, 1252, 1734, 1744
Hpy188I TCNGA 7 cut(s) 88, 357, 451, 532, 659, 1087, 1408
Hpy188III TCNNGA 6 cut(s) 10, 192, 322, 1124, 1145, 1385
Hpy8I GTNNAC 8 cut(s) 152, 223, 793, 1081, 1177, 1252, 1734, 1744
Hpy99I CGWCG 1 cut(s) 725
HpyAV CCTTC 6 cut(s) 319, 884, 1121, 1284, 1421, 1532
HpyCH4III ACNGT 2 cut(s) 289, 1549
HpyCH4IV ACGT 2 cut(s) 1023, 1746
HpyF10VI GCNNNNNNNGC 6 cut(s) 80, 560, 569, 575, 1106, 1731
HpyF3I CTNAG 7 cut(s) 190, 565, 616, 690, 1341, 1479, 1761
HpySE526I ACGT 2 cut(s) 1023, 1746
Hsp92I GRCGYC 1 cut(s) 1725
HspAI GCGC 4 cut(s) 181, 469, 471, 1725
KasI GGCGCC 1 cut(s) 1724
Kpn2I TCCGGA 1 cut(s) 1123
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 4 cut(s) 143, 253, 330, 451
LmnI GCTCC 5 cut(s) 15, 308, 466, 1229, 1421
Lsp1109I GCAGC 8 cut(s) 5, 86, 541, 556, 563, 724, 1087, 1729
LweI GCATC 3 cut(s) 235, 676, 1252
MabI ACCWGGT 1 cut(s) 225
MaeI CTAG 5 cut(s) 35, 44, 209, 1073, 1463
MaeII ACGT 2 cut(s) 1023, 1746
MaeIII GTNAC 5 cut(s) 185, 289, 713, 1051, 1167
MalI GATC 4 cut(s) 145, 255, 332, 453
MbiI CCGCTC 1 cut(s) 1416
MboI GATC 4 cut(s) 143, 253, 330, 451
MboII GAAGA 6 cut(s) 588, 912, 1061, 1280, 1465, 1690
MfeI CAATTG 2 cut(s) 1117, 1789
MflI RGATCY 1 cut(s) 143
MhlI GDGCHC 4 cut(s) 383, 1254, 1372, 1736
MluCI AATT 9 cut(s) 27, 263, 347, 507, 1117, 1134, 1443, 1517, 1789
Mly113I GGCGCC 1 cut(s) 1725
MlyI GAGTC 2 cut(s) 180, 182
MmeI TCCRAC 4 cut(s) 211, 1282, 1443, 1691
MroI TCCGGA 1 cut(s) 1123
MseI TTAA 2 cut(s) 198, 1493
MslI CAYNNNNRTG 1 cut(s) 1260
MspA1I CMGCKG 2 cut(s) 554, 569
MspCI CTTAAG 1 cut(s) 197
MspI CCGG 2 cut(s) 525, 1124
MspR9I CCNGG 4 cut(s) 227, 525, 1008, 1722
MunI CAATTG 2 cut(s) 1117, 1789
Mva1269I GAATGC 1 cut(s) 281
MvaI CCWGG 3 cut(s) 227, 1008, 1722
MvnI CGCG 1 cut(s) 471
MwoI GCNNNNNNNGC 6 cut(s) 80, 560, 569, 575, 1106, 1731
NarI GGCGCC 1 cut(s) 1725
NciI CCSGG 1 cut(s) 525
NdeI CATATG 1 cut(s) 1687
NdeII GATC 4 cut(s) 143, 253, 330, 451
NlaIV GGNNCC 3 cut(s) 1231, 1459, 1726
NmeAIII GCCGAG 1 cut(s) 1753
NmuCI GTSAC 3 cut(s) 185, 289, 1167
NspI RCATGY 1 cut(s) 963
PagI TCATGA 1 cut(s) 9
PauI GCGCGC 1 cut(s) 469
PceI AGGCCT 1 cut(s) 1470
PctI GAATGC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 654, 674
PflMI CCANNNNNTGG 2 cut(s) 375, 1542
PkrI GCNGC 9 cut(s) 20, 76, 553, 556, 571, 739, 742, 1102, 1719
PleI GAGTC 2 cut(s) 179, 182
PluTI GGCGCC 1 cut(s) 1728
PpsI GAGTC 2 cut(s) 179, 182
Ppu21I YACGTR 1 cut(s) 1024
PpuMI RGGWCCY 2 cut(s) 1150, 1382
PsiI TTATAA 1 cut(s) 504
Psp5II RGGWCCY 2 cut(s) 1150, 1382
Psp6I CCWGG 3 cut(s) 225, 1006, 1720
PspGI CCWGG 3 cut(s) 225, 1006, 1720
PspN4I GGNNCC 3 cut(s) 1231, 1459, 1726
PspPI GGNCC 7 cut(s) 223, 366, 1150, 1382, 1457, 1646, 1757
PspPPI RGGWCCY 2 cut(s) 1150, 1382
PstI CTGCAG 3 cut(s) 553, 975, 1719
PstNI CAGNNNCTG 1 cut(s) 80
PsuI RGATCY 1 cut(s) 143
PteI GCGCGC 1 cut(s) 469
PvuII CAGCTG 2 cut(s) 554, 569
RsaI GTAC 1 cut(s) 1713
RsaNI GTAC 1 cut(s) 1712
RseI CAYNNNNRTG 1 cut(s) 1260
SaqAI TTAA 2 cut(s) 198, 1493
SatI GCNGC 9 cut(s) 19, 75, 552, 555, 570, 738, 741, 1101, 1718
Sau3AI GATC 4 cut(s) 143, 253, 330, 451
Sau96I GGNCC 7 cut(s) 223, 366, 1150, 1382, 1457, 1646, 1757
ScaI AGTACT 1 cut(s) 1713
SchI GAGTC 2 cut(s) 180, 182
ScrFI CCNGG 4 cut(s) 227, 525, 1008, 1722
SduI GDGCHC 4 cut(s) 383, 1254, 1372, 1736
SexAI ACCWGGT 1 cut(s) 225
SfaNI GCATC 3 cut(s) 235, 676, 1252
SfcI CTRYAG 4 cut(s) 549, 971, 1484, 1715
SfoI GGCGCC 1 cut(s) 1726
SinI GGWCC 4 cut(s) 223, 1150, 1382, 1457
SmiMI CAYNNNNRTG 1 cut(s) 1260
SmlI CTYRAG 4 cut(s) 197, 213, 779, 1143
SmoI CTYRAG 4 cut(s) 197, 213, 779, 1143
SpeI ACTAGT 1 cut(s) 34
Sse9I AATT 9 cut(s) 27, 263, 347, 507, 1117, 1134, 1443, 1517, 1789
SseBI AGGCCT 1 cut(s) 1470
SsiI CCGC 3 cut(s) 740, 1336, 1414
SspDI GGCGCC 1 cut(s) 1724
SspMI CTAG 5 cut(s) 35, 44, 209, 1073, 1463
StuI AGGCCT 1 cut(s) 1470
StyD4I CCNGG 4 cut(s) 225, 523, 1006, 1720
StyI CCWWGG 2 cut(s) 558, 1642
TaaI ACNGT 2 cut(s) 289, 1549
TaiI ACGT 2 cut(s) 1026, 1749
TaqI TCGA 1 cut(s) 1570
TasI AATT 9 cut(s) 27, 263, 347, 507, 1117, 1134, 1443, 1517, 1789
TatI WGTACW 1 cut(s) 1711
TauI GCSGC 1 cut(s) 743
TfiI GAWTC 2 cut(s) 654, 674
Tru1I TTAA 2 cut(s) 198, 1493
Tru9I TTAA 2 cut(s) 198, 1493
TscAI CASTG 7 cut(s) 85, 190, 294, 388, 930, 1345, 1459
TseFI GTSAC 3 cut(s) 185, 289, 1167
TseI GCWGC 8 cut(s) 18, 74, 551, 554, 569, 737, 1100, 1717
Tsp45I GTSAC 3 cut(s) 185, 289, 1167
TspDTI ATGAA 8 cut(s) 291, 382, 528, 611, 687, 1235, 1280, 1440
TspRI CASTG 7 cut(s) 85, 190, 294, 388, 930, 1345, 1459
Van91I CCANNNNNTGG 2 cut(s) 375, 1542
Vha464I CTTAAG 1 cut(s) 197
VneI GTGCAC 2 cut(s) 1250, 1732
VpaK11BI GGWCC 4 cut(s) 223, 1150, 1382, 1457
XapI RAATTY 3 cut(s) 263, 1134, 1517
XceI RCATGY 1 cut(s) 963
XcmI CCANNNNNNNNNTGG 1 cut(s) 1004
XspI CTAG 5 cut(s) 35, 44, 209, 1073, 1463
ZrmI AGTACT 1 cut(s) 1713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.