RLG00000002135

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0013278
Physical Location & Seq
Reverse (-)
26083408 .. 26086363
2956 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002135

Sequence Viewer

Length: 1878 bp
ATGATGTTCATGAGGAGCTGCACCAAAATTGCCCTAGTCTCCCTAGCTTTGCTAATGCTACTTGGTGTGGAAGCAGCCACTGCTTCAGAGTTTAACTACGGCGAGGCTTTAGACAAGAGTTTGCTCTTTTTCGAGGCACAACGATCTGGTAAACTGCCCCCAAACCAGCGAGTGAAGTGGCGCAGTGACTCAGGACTTAAGGATGGCCTAGCTCAAGGGGTGGACCTGGTTGGAGGGTATTATGATGCAGGGGATCATGTGAAATTTGGACTACCAATGGCATTCACAGTGACAATGCTTTCATGGGGAGCTATTGAGTTCAGGAAGGAGATCACAGATTTAGACCAATTAGGTCAGACATTATGGGCCATTAGATGGGGCACTGACTATTTCATCAAAGCACATCCACAGCCTAATGTTCTTTGGGGACAGGTAGGAGATGGGAACTCTGATCACTATTGTTGGGAGCGCGCAGAGGACATGACAACTCCAAGAAATGCTTACAAAATTGATGAAAATCATCCCGGTTCAGACATTGCTGGTGAAACTGCAGCTGCCTTGGCCTCAGCTGCCATAGCTTTCAAGCCTTACAACTCTTCCTACTCCAATCTCCTCTTAGTTCATGCAAAACAGCTTTTCTCATTTGCAGATAGATTCCGAGGATTATATGATGAATCCATTGAAGATGCTAAGGACTTCTACACCTCATCGGGTTACTCCGATGAGCTACTATGGGCTGCGGCATGGCTCTACGAAGCAACCAACGATGACTACTACCTCAAGTATGTGGTGGACAACGCTGTGTATATGGGTGGAACTGGATGGTCAGTCAAAGAGTTTTCTTGGGATAACAAATATGCTGGTGTCCAAATCCTTCTCACAAAGGTTTTGTTAGAGGGAAGAGGTGGTGCATATACTTCCACACTGAAACAATATCAGGACAAAGCAGATTACTTTGCCTGTGCTTGTCTGCAAAAAAATGATGGCTACAATGTCCGCACAACTCCTGGGGGCTTATTATACGTGCGTGAATGGAACAACTTGCAGTATGTTACTTCTGCTTCGTTTCTTCTAGCTGTTTACTCCGATTATCTCTCTGCTGCAAATGCCAAGCTCAATTGTCCTGAAGGACAAATTCAACCTCAAGAGGTCCTCAACTTTGCAAAGTCACAGGTTGACTACATTCTTGGTAAAAACCCCAAGTCAATGAGCTACATAGTTGGGTATGGAGCAAAGTATCCGGTTCATGTACACCACAGAGATGCTTCCATTCCTTCTATATCAGTTCTTCATGCTGTGGTCGGATGTGTGCAAGGATTTGAGATATGGTATCACCGCACTGAGGGAAATCCTAATGTCGTCTATGGGGCACTTGTGGGAGGTCCTGACCAGAATGACAACTTCTCCGACGACCGCTCCAACTATGAAGGGACAGAGCCTACAATTTCTGGCATTGGTCCCCTAGTAGGCCTCTTCTCTAAGCTACAGAGTTTAAATGGTGGTTTCAATAGAGGATCACCAAAATCCCATACACCCACACCAAGTGCCTATCACCCGGAAACAACAGCACCCCCAGAAAAATCAGATACTCCTCCTATCAAATTCCTTCATTCTGTAACTAGCACATGGACTGTTGCAAAAACAAGTTATTATCGACACAAGGTGATACTAAAGAACACATCCCAGAAACCGATAACAAATGTGAAGCTGGTGCTTGGAAACCTCCAAGGGCCATTATGGGGTCTCTCTCCAACTCCACAGAAGAACACATATGAGCTTCCTCAATGGCAGAAAGTTCTGCAGCCTGGAGCCGAGTGCACTTTTGTTTACGTTCAAGGTGGCCCTCAGGCAAAGATATCTATCCAAAGCTACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
CBM49 Glyco_hydro_9

Protein Analysis

626

Amino Acids

69.31

Weight (kDa)

6.08

Isoelectric Point (pI)

31.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 33 - 491 1.3e-168 Glycosyl hydrolase family 9
CBM49 PF09478 533 - 612 2.8e-22 Carbohydrate binding domain CBM49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48930
fragaria_vesca FvH4_5g29090
malus_domestica MD16G1001700.v1.1
prunus_persica Prupe.1G346900_v2.0.a1
pyrus_communis pycom16g00390
rosa_chinensis RchiOBHm_Chr7g0221721 RchiOBHm_Chr7g0221731
rosa_laevigata RLG00000002135
rosa_multiflora Rmu_co8400965.1_g000001 Rmu_sc0003311.1_g000002
rosa_roxburghii Rroxscaffold_3G00238090
rosa_rugosa Rorug07G0203000
rosa_samantha Rh7AG343900 Rh7BG334300 Rh7CG361300 Rh7DG341700
rosa_wichuraiana Rw0G003250 Rw7G029160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 375
AccBSI CCGCTC 1 cut(s) 1416
AccII CGCG 1 cut(s) 471
AciI CCGC 4 cut(s) 740, 997, 1336, 1414
AclWI GGATC 2 cut(s) 261, 1522
AcsI RAATTY 3 cut(s) 263, 1134, 1601
AcuI CTGAAG 2 cut(s) 69, 1146
AdeI CACNNNGTG 2 cut(s) 1544, 1663
AfaI GTAC 1 cut(s) 1251
AfiI CCNNNNNNNGG 4 cut(s) 375, 1342, 1466, 1739
AflII CTTAAG 1 cut(s) 197
AgsI TTSAA 5 cut(s) 583, 683, 1139, 1507, 1835
AjnI CCWGG 3 cut(s) 225, 1006, 1804
Alw21I GWGCWC 1 cut(s) 1820
Alw26I GTCTC 2 cut(s) 43, 1748
Alw44I GTGCAC 1 cut(s) 1816
AlwI GGATC 2 cut(s) 261, 1522
AlwNI CAGNNNCTG 1 cut(s) 80
AoxI GGCC 6 cut(s) 205, 366, 561, 1468, 1730, 1840
ApaLI GTGCAC 1 cut(s) 1816
ApeKI GCWGC 8 cut(s) 18, 74, 551, 554, 569, 737, 1100, 1801
ApoI RAATTY 3 cut(s) 263, 1134, 1601
AspLEI GCGC 3 cut(s) 183, 471, 473
AspS9I GGNCC 7 cut(s) 223, 366, 1150, 1382, 1457, 1730, 1841
AsuC2I CCSGG 2 cut(s) 525, 1556
AsuHPI GGTGA 5 cut(s) 554, 1325, 1509, 1544, 1675
AvaII GGWCC 4 cut(s) 223, 1150, 1382, 1457
AxyI CCTNAGG 1 cut(s) 1845
BaeGI GKGCMC 3 cut(s) 383, 1372, 1820
Bbv12I GWGCWC 1 cut(s) 1820
BbvCI CCTCAGC 1 cut(s) 565
BbvI GCAGC 8 cut(s) 5, 86, 541, 556, 563, 724, 1087, 1813
BccI CCATC 5 cut(s) 197, 369, 434, 816, 977
BceAI ACGGC 1 cut(s) 115
BciT130I CCWGG 3 cut(s) 227, 1008, 1806
BciVI GTATCC 1 cut(s) 1248
BclI TGATCA 1 cut(s) 451
BcnI CCSGG 2 cut(s) 525, 1556
BcoDI GTCTC 2 cut(s) 43, 1748
BfaI CTAG 6 cut(s) 35, 44, 209, 1073, 1463, 1620
BfmI CTRYAG 3 cut(s) 549, 1484, 1799
BfrI CTTAAG 1 cut(s) 197
BfuI GTATCC 1 cut(s) 1248
BisI GCNGC 9 cut(s) 19, 75, 552, 555, 570, 738, 741, 1101, 1802
BlsI GCNGC 9 cut(s) 20, 76, 553, 556, 571, 739, 742, 1102, 1803
Bme1390I CCNGG 5 cut(s) 227, 525, 1008, 1556, 1806
Bme18I GGWCC 4 cut(s) 223, 1150, 1382, 1457
BmgT120I GGNCC 7 cut(s) 223, 366, 1150, 1382, 1457, 1730, 1841
BmiI GGNNCC 2 cut(s) 1459, 1810
BmrFI CCNGG 5 cut(s) 227, 525, 1008, 1556, 1806
BmsI GCATC 3 cut(s) 235, 676, 1252
BpmI CTGGAG 1 cut(s) 1827
Bpu10I CCTNAGC 2 cut(s) 565, 690
BpuEI CTTGAG 3 cut(s) 198, 764, 1128
BpuMI CCSGG 2 cut(s) 525, 1556
BsaAI YACGTR 1 cut(s) 1024
BsaBI GATNNNNATC 2 cut(s) 516, 1859
BsaI GGTCTC 1 cut(s) 1748
BsaJI CCNNGG 4 cut(s) 558, 658, 1007, 1726
BsaWI WCCGGW 1 cut(s) 1240
BsaXI ACNNNNNCTCC 4 cut(s) 1388, 1400, 1418, 1430
Bsc4I CCNNNNNNNGG 4 cut(s) 375, 1342, 1466, 1739
Bse1I ACTGG 1 cut(s) 823
Bse21I CCTNAGG 1 cut(s) 1845
Bse3DI GCAATG 1 cut(s) 534
Bse8I GATNNNNATC 2 cut(s) 516, 1859
BseBI CCWGG 3 cut(s) 227, 1008, 1806
BseDI CCNNGG 4 cut(s) 558, 658, 1007, 1726
BseGI GGATG 6 cut(s) 208, 403, 520, 827, 1310, 1679
BseJI GATNNNNATC 2 cut(s) 516, 1859
BseLI CCNNNNNNNGG 4 cut(s) 375, 1342, 1466, 1739
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 4 cut(s) 204, 579, 1332, 1859
BseNI ACTGG 1 cut(s) 823
BsePI GCGCGC 1 cut(s) 469
BseRI GAGGAG 3 cut(s) 28, 602, 1581
BseSI GKGCMC 3 cut(s) 383, 1372, 1820
BseXI GCAGC 8 cut(s) 5, 86, 541, 556, 563, 724, 1087, 1813
BsgI GTGCAG 1 cut(s) 4
Bsh1236I CGCG 1 cut(s) 471
Bsh1285I CGRYCG 1 cut(s) 1414
BshFI GGCC 6 cut(s) 207, 368, 563, 1470, 1732, 1842
BsiEI CGRYCG 1 cut(s) 1414
BsiHKAI GWGCWC 1 cut(s) 1820
BsiSI CCGG 3 cut(s) 525, 1241, 1556
BslFI GGGAC 3 cut(s) 441, 1443, 1444
BslI CCNNNNNNNGG 4 cut(s) 375, 1342, 1466, 1739
BsmAI GTCTC 2 cut(s) 43, 1748
BsmFI GGGAC 3 cut(s) 441, 1443, 1444
BsmI GAATGC 1 cut(s) 281
BsnI GGCC 6 cut(s) 207, 368, 563, 1470, 1732, 1842
Bso31I GGTCTC 1 cut(s) 1748
Bsp1286I GDGCHC 3 cut(s) 383, 1372, 1820
Bsp1407I TGTACA 1 cut(s) 1249
Bsp143I GATC 5 cut(s) 143, 253, 330, 451, 1514
BspACI CCGC 4 cut(s) 740, 997, 1336, 1414
BspANI GGCC 6 cut(s) 207, 368, 563, 1470, 1732, 1842
BspCNI CTCAG 4 cut(s) 203, 578, 1333, 1858
BspFNI CGCG 1 cut(s) 471
BspHI TCATGA 1 cut(s) 9
BspLI GGNNCC 2 cut(s) 1459, 1810
BspMAI CTGCAG 2 cut(s) 553, 1803
BspPI GGATC 2 cut(s) 261, 1522
BspTI CTTAAG 1 cut(s) 197
BspTNI GGTCTC 1 cut(s) 1748
BsrBI CCGCTC 1 cut(s) 1416
BsrDI GCAATG 1 cut(s) 534
BsrGI TGTACA 1 cut(s) 1249
BsrI ACTGG 1 cut(s) 823
BssECI CCNNGG 4 cut(s) 558, 658, 1007, 1726
BssHII GCGCGC 1 cut(s) 469
BssMI GATC 5 cut(s) 143, 253, 330, 451, 1514
BssT1I CCWWGG 2 cut(s) 558, 1726
Bst2UI CCWGG 3 cut(s) 227, 1008, 1806
Bst4CI ACNGT 2 cut(s) 289, 1633
Bst6I CTCTTC 3 cut(s) 601, 895, 1478
BstAFI CTTAAG 1 cut(s) 197
BstAPI GCANNNNNTGC 1 cut(s) 80
BstAUI TGTACA 1 cut(s) 1249
BstBAI YACGTR 1 cut(s) 1024
BstC8I GCNNGC 1 cut(s) 471
BstDEI CTNAG 7 cut(s) 190, 565, 616, 690, 1341, 1479, 1845
BstF5I GGATG 6 cut(s) 208, 403, 520, 827, 1310, 1679
BstFNI CGCG 1 cut(s) 471
BstHHI GCGC 3 cut(s) 183, 471, 473
BstKTI GATC 5 cut(s) 146, 256, 333, 454, 1517
BstMAI GTCTC 2 cut(s) 43, 1748
BstMBI GATC 5 cut(s) 143, 253, 330, 451, 1514
BstMCI CGRYCG 1 cut(s) 1414
BstMWI GCNNNNNNNGC 5 cut(s) 80, 560, 569, 575, 1106
BstNI CCWGG 3 cut(s) 227, 1008, 1806
BstSCI CCNGG 5 cut(s) 225, 523, 1006, 1554, 1804
BstSFI CTRYAG 3 cut(s) 549, 1484, 1799
BstSLI GKGCMC 3 cut(s) 383, 1372, 1820
BstUI CGCG 1 cut(s) 471
BstV1I GCAGC 8 cut(s) 5, 86, 541, 556, 563, 724, 1087, 1813
Bsu36I CCTNAGG 1 cut(s) 1845
BsuI GTATCC 1 cut(s) 1248
BsuRI GGCC 6 cut(s) 207, 368, 563, 1470, 1732, 1842
BtsCI GGATG 6 cut(s) 208, 403, 520, 827, 1310, 1679
BtsI GCAGTG 2 cut(s) 78, 190
BtsIMutI CAGTG 6 cut(s) 78, 190, 294, 381, 923, 1338
Cac8I GCNNGC 1 cut(s) 471
CaiI CAGNNNCTG 1 cut(s) 80
CciI TCATGA 1 cut(s) 9
CfoI GCGC 3 cut(s) 183, 471, 473
Cfr13I GGNCC 7 cut(s) 223, 366, 1150, 1382, 1457, 1730, 1841
CsiI ACCWGGT 1 cut(s) 225
Csp6I GTAC 1 cut(s) 1250
CviAII CATG 9 cut(s) 10, 257, 303, 481, 623, 744, 1247, 1292, 1626
CviQI GTAC 1 cut(s) 1250
DdeI CTNAG 7 cut(s) 190, 565, 616, 690, 1341, 1479, 1845
DpnI GATC 5 cut(s) 145, 255, 332, 453, 1516
DpnII GATC 5 cut(s) 143, 253, 330, 451, 1514
DraI TTTAAA 1 cut(s) 1494
DraIII CACNNNGTG 2 cut(s) 1544, 1663
Eam1104I CTCTTC 3 cut(s) 601, 895, 1478
EarI CTCTTC 3 cut(s) 601, 895, 1478
Eco130I CCWWGG 2 cut(s) 558, 1726
Eco147I AGGCCT 1 cut(s) 1470
Eco31I GGTCTC 1 cut(s) 1748
Eco32I GATATC 1 cut(s) 1857
Eco47I GGWCC 4 cut(s) 223, 1150, 1382, 1457
Eco57I CTGAAG 2 cut(s) 69, 1146
Eco81I CCTNAGG 1 cut(s) 1845
EcoO109I RGGNCCY 2 cut(s) 1150, 1382
EcoRII CCWGG 3 cut(s) 225, 1006, 1804
EcoRV GATATC 1 cut(s) 1857
EcoT14I CCWWGG 2 cut(s) 558, 1726
ErhI CCWWGG 2 cut(s) 558, 1726
FaeI CATG 9 cut(s) 13, 260, 306, 484, 626, 747, 1250, 1295, 1629
FalI AAGNNNNNCTT 2 cut(s) 484, 516
FaqI GGGAC 3 cut(s) 441, 1443, 1444
FatI CATG 9 cut(s) 9, 256, 302, 480, 622, 743, 1246, 1291, 1625
FauNDI CATATG 1 cut(s) 1771
FbaI TGATCA 1 cut(s) 451
Fnu4HI GCNGC 9 cut(s) 19, 75, 552, 555, 570, 738, 741, 1101, 1802
FokI GGATG 6 cut(s) 215, 390, 507, 834, 1317, 1666
Fsp4HI GCNGC 9 cut(s) 19, 75, 552, 555, 570, 738, 741, 1101, 1802
FspBI CTAG 6 cut(s) 35, 44, 209, 1073, 1463, 1620
GlaI GCGC 3 cut(s) 182, 470, 472
GluI GCNGC 9 cut(s) 19, 75, 552, 555, 570, 738, 741, 1101, 1802
GsuI CTGGAG 1 cut(s) 1827
HaeIII GGCC 6 cut(s) 207, 368, 563, 1470, 1732, 1842
HapII CCGG 3 cut(s) 525, 1241, 1556
HhaI GCGC 3 cut(s) 183, 471, 473
Hin1II CATG 9 cut(s) 13, 260, 306, 484, 626, 747, 1250, 1295, 1629
Hin6I GCGC 3 cut(s) 181, 469, 471
HinP1I GCGC 3 cut(s) 181, 469, 471
HincII GTYRAC 1 cut(s) 1177
HindII GTYRAC 1 cut(s) 1177
HinfI GANTC 3 cut(s) 188, 654, 674
HpaII CCGG 3 cut(s) 525, 1241, 1556
HphI GGTGA 5 cut(s) 554, 1325, 1509, 1544, 1675
Hpy166II GTNNAC 8 cut(s) 152, 223, 793, 1081, 1177, 1252, 1818, 1828
Hpy188III TCNNGA 7 cut(s) 10, 192, 322, 938, 1124, 1145, 1385
Hpy8I GTNNAC 8 cut(s) 152, 223, 793, 1081, 1177, 1252, 1818, 1828
Hpy99I CGWCG 1 cut(s) 1412
HpyAV CCTTC 6 cut(s) 319, 884, 1121, 1284, 1421, 1616
HpyCH4III ACNGT 2 cut(s) 289, 1633
HpyCH4IV ACGT 2 cut(s) 1023, 1830
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 560, 569, 575, 1106
HpyF3I CTNAG 7 cut(s) 190, 565, 616, 690, 1341, 1479, 1845
HpySE526I ACGT 2 cut(s) 1023, 1830
Hsp92II CATG 9 cut(s) 13, 260, 306, 484, 626, 747, 1250, 1295, 1629
HspAI GCGC 3 cut(s) 181, 469, 471
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 5 cut(s) 143, 253, 330, 451, 1514
LmnI GCTCC 6 cut(s) 15, 308, 466, 1229, 1421, 1808
Lsp1109I GCAGC 8 cut(s) 5, 86, 541, 556, 563, 724, 1087, 1813
LweI GCATC 3 cut(s) 235, 676, 1252
MabI ACCWGGT 1 cut(s) 225
MaeI CTAG 6 cut(s) 35, 44, 209, 1073, 1463, 1620
MaeII ACGT 2 cut(s) 1023, 1830
MaeIII GTNAC 6 cut(s) 185, 289, 713, 1051, 1167, 1615
MalI GATC 5 cut(s) 145, 255, 332, 453, 1516
MbiI CCGCTC 1 cut(s) 1416
MboI GATC 5 cut(s) 143, 253, 330, 451, 1514
MboII GAAGA 7 cut(s) 588, 695, 912, 1061, 1280, 1465, 1774
MfeI CAATTG 2 cut(s) 1117, 1873
MhlI GDGCHC 3 cut(s) 383, 1372, 1820
MluCI AATT 9 cut(s) 27, 263, 347, 507, 1117, 1134, 1443, 1601, 1873
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 5 cut(s) 211, 1282, 1431, 1443, 1775
MseI TTAA 3 cut(s) 93, 198, 1493
MslI CAYNNNNRTG 1 cut(s) 1260
MspA1I CMGCKG 2 cut(s) 554, 569
MspCI CTTAAG 1 cut(s) 197
MspI CCGG 3 cut(s) 525, 1241, 1556
MspR9I CCNGG 5 cut(s) 227, 525, 1008, 1556, 1806
MunI CAATTG 2 cut(s) 1117, 1873
Mva1269I GAATGC 1 cut(s) 281
MvaI CCWGG 3 cut(s) 227, 1008, 1806
MvnI CGCG 1 cut(s) 471
MwoI GCNNNNNNNGC 5 cut(s) 80, 560, 569, 575, 1106
NciI CCSGG 2 cut(s) 525, 1556
NdeI CATATG 1 cut(s) 1771
NdeII GATC 5 cut(s) 143, 253, 330, 451, 1514
NlaIII CATG 9 cut(s) 13, 260, 306, 484, 626, 747, 1250, 1295, 1629
NlaIV GGNNCC 2 cut(s) 1459, 1810
NmeAIII GCCGAG 1 cut(s) 1837
NmuCI GTSAC 3 cut(s) 185, 289, 1167
PagI TCATGA 1 cut(s) 9
PauI GCGCGC 1 cut(s) 469
PceI AGGCCT 1 cut(s) 1470
PctI GAATGC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 654, 674
PflMI CCANNNNNTGG 1 cut(s) 375
PkrI GCNGC 9 cut(s) 20, 76, 553, 556, 571, 739, 742, 1102, 1803
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
Ppu21I YACGTR 1 cut(s) 1024
PpuMI RGGWCCY 2 cut(s) 1150, 1382
Psp5II RGGWCCY 2 cut(s) 1150, 1382
Psp6I CCWGG 3 cut(s) 225, 1006, 1804
PspGI CCWGG 3 cut(s) 225, 1006, 1804
PspN4I GGNNCC 2 cut(s) 1459, 1810
PspPI GGNCC 7 cut(s) 223, 366, 1150, 1382, 1457, 1730, 1841
PspPPI RGGWCCY 2 cut(s) 1150, 1382
PstI CTGCAG 2 cut(s) 553, 1803
PstNI CAGNNNCTG 1 cut(s) 80
PteI GCGCGC 1 cut(s) 469
PvuII CAGCTG 2 cut(s) 554, 569
RsaI GTAC 1 cut(s) 1251
RsaNI GTAC 1 cut(s) 1250
RseI CAYNNNNRTG 1 cut(s) 1260
SaqAI TTAA 3 cut(s) 93, 198, 1493
SatI GCNGC 9 cut(s) 19, 75, 552, 555, 570, 738, 741, 1101, 1802
Sau3AI GATC 5 cut(s) 143, 253, 330, 451, 1514
Sau96I GGNCC 7 cut(s) 223, 366, 1150, 1382, 1457, 1730, 1841
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 5 cut(s) 227, 525, 1008, 1556, 1806
SduI GDGCHC 3 cut(s) 383, 1372, 1820
SexAI ACCWGGT 1 cut(s) 225
SfaNI GCATC 3 cut(s) 235, 676, 1252
SfcI CTRYAG 3 cut(s) 549, 1484, 1799
SinI GGWCC 4 cut(s) 223, 1150, 1382, 1457
SmiMI CAYNNNNRTG 1 cut(s) 1260
SmlI CTYRAG 4 cut(s) 197, 213, 779, 1143
SmoI CTYRAG 4 cut(s) 197, 213, 779, 1143
Sse9I AATT 9 cut(s) 27, 263, 347, 507, 1117, 1134, 1443, 1601, 1873
SseBI AGGCCT 1 cut(s) 1470
SsiI CCGC 4 cut(s) 740, 997, 1336, 1414
SspMI CTAG 6 cut(s) 35, 44, 209, 1073, 1463, 1620
StuI AGGCCT 1 cut(s) 1470
StyD4I CCNGG 5 cut(s) 225, 523, 1006, 1554, 1804
StyI CCWWGG 2 cut(s) 558, 1726
TaaI ACNGT 2 cut(s) 289, 1633
TaiI ACGT 2 cut(s) 1026, 1833
TaqI TCGA 2 cut(s) 132, 1654
TasI AATT 9 cut(s) 27, 263, 347, 507, 1117, 1134, 1443, 1601, 1873
TatI WGTACW 1 cut(s) 1249
TauI GCSGC 1 cut(s) 743
TfiI GAWTC 2 cut(s) 654, 674
Tru1I TTAA 3 cut(s) 93, 198, 1493
Tru9I TTAA 3 cut(s) 93, 198, 1493
TscAI CASTG 6 cut(s) 85, 190, 294, 388, 930, 1345
TseFI GTSAC 3 cut(s) 185, 289, 1167
TseI GCWGC 8 cut(s) 18, 74, 551, 554, 569, 737, 1100, 1801
Tsp45I GTSAC 3 cut(s) 185, 289, 1167
TspDTI ATGAA 9 cut(s) 291, 382, 528, 611, 687, 1235, 1280, 1440, 1598
TspRI CASTG 6 cut(s) 85, 190, 294, 388, 930, 1345
Van91I CCANNNNNTGG 1 cut(s) 375
Vha464I CTTAAG 1 cut(s) 197
VneI GTGCAC 1 cut(s) 1816
VpaK11BI GGWCC 4 cut(s) 223, 1150, 1382, 1457
XapI RAATTY 3 cut(s) 263, 1134, 1601
XspI CTAG 6 cut(s) 35, 44, 209, 1073, 1463, 1620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.