Rroxscaffold_3G00238090

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013278
Physical Location & Seq
Reverse (-)
26219567 .. 26222282
2716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00238090.1

Sequence Viewer

Length: 1617 bp
ATGATGTTCATGAGGAGCAGCACCAAAATTGCACTAGTCTCCCTAGCTTTGCTAATGCTACTTGGTGTGGAAGCAGCCACTGCTTCAGAGTTTAACTACGGCGAGGCTTTAGACAAGAGTTTGCTCTTTTTTGAGGCACAAAGATCTGGTAAACTGCCCCCAAACCAGCGAGTCAAGTGGCGCAGTGACTCAGGACTTGAGGATGGCCTAGCTCAAGGGGTGGACCTGGTTGGAGGGTATTATGATGCAGGGGATCATGTGAAATTTGGACTACCAATGGCATTCACAGTGACAATGCTTTCATGGGGAGCTATTGAGTTCAGGAAGGAGATCACAGATTTAGACCAACTAGGTCAGACATTATGGGCCATTAGATGGGGCACTGACTATTTCATCAAAGCACATCCACAGCCTAATGTTCTTTGGGGACAGGTAGGAGATGGGAACTCTGATCACTATTGTTGGGAGCGCGCAGAGGACATGACAACTCCAAGAAATGCTTACAAAATTGATGAAAATCATCCCGGTTCAGACATTGCTGGTGAAACTGCAGCTGCCTTGGCCTCAGCTGCCATAGCTTTCAAGCCTTACAACTCTTCCTACTCCAATCTCCTCCTAGTTCATGCAAAACAGCTTTTCTCATTTGCAGATAGATTCCGAGGATTATATGATGAATCCATTGAAGATGCTAAGGACTTCTACACCTCATCGGGACGAGCTACTATGGGCTGCGGCATGGCTCTCTACGAAGCAACCAATGATGACTACTACCTCAAGTATGTGGTGGACAACGCCGTGTATATGGGTGGAACTGGATGGTCAGTCAAAGAGTTTTCTTGGGATAACAAATATGCTGGTGTCCAAATCCTTCTCACAAAGGTTTTGTTAGAGGGAAGAGGTGGTGCATATACTTCCACACTGAAACAATATCAGGCCAAAGCAGATTACTTTGCCTGTGCTTGTCTGCAGAAAAATGATGGCTACAATGTCCATACAACTCCTGGGGGCTTATTATACGTGCGTGAATGGAACAACTTGCAGTATGTTACTTCTGCTTCGTTTCTTCTAGCTGTTTACTCCGATTATCTCTCTGCTGCAAATGCCAAGCTCAATTGTCCGGAAGGACAAATTCAACCTCAAGAGGTCCTCAACTTTGCAAAGTCACAGGTTGACTACATTCTTGGTAAAAACCCCAAGTCAATGAGCTACATAGTTGGGTATGGAGCAAAGTATCCGGTTCATGTACACCACAGAGATGCTTCCATTCCTTCTATATCAGTTCTTCATGCTGTGGTTGGATGTGTGCAAGGATTTGAGATATGGTATCACCGCACTGAGGGAAATCCTAATGTCGTCTATGGGGCACTTGTTGGAGGTCCTGACCAGAATGACAACTTCTCCGATGACCGCTCCAACTATGAAGGGACAGAGCCTACAATTTCTGGCACTGGTCCCCTAGTAGGCCTCTTCTCTAAGCTACAGAGTTTAAATGGTGGTTTCAATAGAGGATCACCGAAATCCCATACACCCACACCAAGAGCCTATCACCCTGAAACAACAGCACCCCCAACAAAAATCAGATACTCCTCCTATCAAATTCCTTCACTCTATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
CBM49 Glyco_hydro_9

Protein Analysis

538

Amino Acids

59.46

Weight (kDa)

5.74

Isoelectric Point (pI)

29.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 33 - 489 2e-158 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48930
fragaria_vesca FvH4_5g29090
malus_domestica MD16G1001700.v1.1
prunus_persica Prupe.1G346900_v2.0.a1
pyrus_communis pycom16g00390
rosa_chinensis RchiOBHm_Chr7g0221721 RchiOBHm_Chr7g0221731
rosa_laevigata RLG00000002135
rosa_multiflora Rmu_co8400965.1_g000001 Rmu_sc0003311.1_g000002
rosa_roxburghii Rroxscaffold_3G00238090
rosa_rugosa Rorug07G0203000
rosa_samantha Rh7AG343900 Rh7BG334300 Rh7CG361300 Rh7DG341700
rosa_wichuraiana Rw0G003250 Rw7G029160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 375
AccBSI CCGCTC 1 cut(s) 1410
AccII CGCG 1 cut(s) 471
AccIII TCCGGA 1 cut(s) 1117
AciI CCGC 3 cut(s) 732, 1330, 1408
AclWI GGATC 2 cut(s) 261, 1516
AcsI RAATTY 3 cut(s) 263, 1128, 1596
AcuI CTGAAG 1 cut(s) 69
AfaI GTAC 1 cut(s) 1245
AfiI CCNNNNNNNGG 3 cut(s) 375, 1336, 1460
AgsI TTSAA 4 cut(s) 583, 683, 1133, 1501
AhlI ACTAGT 1 cut(s) 34
AjnI CCWGG 2 cut(s) 225, 1000
Alw26I GTCTC 1 cut(s) 43
AlwI GGATC 2 cut(s) 261, 1516
AlwNI CAGNNNCTG 1 cut(s) 80
Aor13HI TCCGGA 1 cut(s) 1117
AoxI GGCC 5 cut(s) 205, 366, 561, 933, 1462
ApeKI GCWGC 7 cut(s) 18, 74, 551, 554, 569, 729, 1094
ApoI RAATTY 3 cut(s) 263, 1128, 1596
AspLEI GCGC 3 cut(s) 183, 471, 473
AspS9I GGNCC 5 cut(s) 223, 366, 1144, 1376, 1451
AsuC2I CCSGG 1 cut(s) 525
AsuHPI GGTGA 4 cut(s) 554, 1319, 1503, 1538
AvaII GGWCC 4 cut(s) 223, 1144, 1376, 1451
BaeGI GKGCMC 2 cut(s) 383, 1366
BbvCI CCTCAGC 1 cut(s) 565
BbvI GCAGC 7 cut(s) 30, 86, 541, 556, 563, 716, 1081
BccI CCATC 5 cut(s) 197, 369, 434, 810, 971
BceAI ACGGC 2 cut(s) 115, 779
BciT130I CCWGG 2 cut(s) 227, 1002
BciVI GTATCC 1 cut(s) 1242
BclI TGATCA 1 cut(s) 451
BcnI CCSGG 1 cut(s) 525
BcoDI GTCTC 1 cut(s) 43
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 8 cut(s) 35, 44, 209, 350, 617, 1067, 1457, 1615
BfmI CTRYAG 3 cut(s) 549, 965, 1478
BfuI GTATCC 1 cut(s) 1242
BglII AGATCT 1 cut(s) 143
BisI GCNGC 8 cut(s) 19, 75, 552, 555, 570, 730, 733, 1095
BlsI GCNGC 8 cut(s) 20, 76, 553, 556, 571, 731, 734, 1096
Bme1390I CCNGG 3 cut(s) 227, 525, 1002
Bme18I GGWCC 4 cut(s) 223, 1144, 1376, 1451
BmgT120I GGNCC 5 cut(s) 223, 366, 1144, 1376, 1451
BmiI GGNNCC 1 cut(s) 1453
BmrFI CCNGG 3 cut(s) 227, 525, 1002
BmsI GCATC 3 cut(s) 235, 676, 1246
Bpu10I CCTNAGC 2 cut(s) 565, 690
BpuEI CTTGAG 4 cut(s) 198, 218, 758, 1122
BpuMI CCSGG 1 cut(s) 525
BsaAI YACGTR 1 cut(s) 1018
BsaBI GATNNNNATC 1 cut(s) 516
BsaJI CCNNGG 3 cut(s) 558, 658, 1001
BsaWI WCCGGW 2 cut(s) 1117, 1234
BsaXI ACNNNNNCTCC 2 cut(s) 1382, 1412
Bsc4I CCNNNNNNNGG 3 cut(s) 375, 1336, 1460
Bse1I ACTGG 2 cut(s) 817, 1453
Bse3DI GCAATG 1 cut(s) 534
Bse8I GATNNNNATC 1 cut(s) 516
BseAI TCCGGA 1 cut(s) 1117
BseBI CCWGG 2 cut(s) 227, 1002
BseDI CCNNGG 3 cut(s) 558, 658, 1001
BseGI GGATG 5 cut(s) 208, 403, 520, 821, 1304
BseJI GATNNNNATC 1 cut(s) 516
BseLI CCNNNNNNNGG 3 cut(s) 375, 1336, 1460
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 3 cut(s) 204, 579, 1326
BseNI ACTGG 2 cut(s) 817, 1453
BsePI GCGCGC 1 cut(s) 469
BseRI GAGGAG 3 cut(s) 28, 602, 1576
BseSI GKGCMC 2 cut(s) 383, 1366
BseXI GCAGC 7 cut(s) 30, 86, 541, 556, 563, 716, 1081
Bsh1236I CGCG 1 cut(s) 471
BshFI GGCC 5 cut(s) 207, 368, 563, 935, 1464
BsiSI CCGG 3 cut(s) 525, 1118, 1235
BslFI GGGAC 4 cut(s) 441, 726, 1437, 1438
BslI CCNNNNNNNGG 3 cut(s) 375, 1336, 1460
BsmAI GTCTC 1 cut(s) 43
BsmFI GGGAC 4 cut(s) 441, 726, 1437, 1438
BsmI GAATGC 1 cut(s) 281
BsnI GGCC 5 cut(s) 207, 368, 563, 935, 1464
Bsp1286I GDGCHC 2 cut(s) 383, 1366
Bsp13I TCCGGA 1 cut(s) 1117
Bsp1407I TGTACA 1 cut(s) 1243
Bsp143I GATC 5 cut(s) 143, 253, 330, 451, 1508
BspACI CCGC 3 cut(s) 732, 1330, 1408
BspANI GGCC 5 cut(s) 207, 368, 563, 935, 1464
BspCNI CTCAG 3 cut(s) 203, 578, 1327
BspEI TCCGGA 1 cut(s) 1117
BspFNI CGCG 1 cut(s) 471
BspHI TCATGA 1 cut(s) 9
BspLI GGNNCC 1 cut(s) 1453
BspMAI CTGCAG 2 cut(s) 553, 969
BspPI GGATC 2 cut(s) 261, 1516
BsrBI CCGCTC 1 cut(s) 1410
BsrDI GCAATG 1 cut(s) 534
BsrGI TGTACA 1 cut(s) 1243
BsrI ACTGG 2 cut(s) 817, 1453
BssECI CCNNGG 3 cut(s) 558, 658, 1001
BssHII GCGCGC 1 cut(s) 469
BssMI GATC 5 cut(s) 143, 253, 330, 451, 1508
BssT1I CCWWGG 1 cut(s) 558
Bst2UI CCWGG 2 cut(s) 227, 1002
Bst4CI ACNGT 1 cut(s) 289
Bst6I CTCTTC 3 cut(s) 601, 889, 1472
BstAPI GCANNNNNTGC 1 cut(s) 80
BstAUI TGTACA 1 cut(s) 1243
BstBAI YACGTR 1 cut(s) 1018
BstC8I GCNNGC 1 cut(s) 471
BstDEI CTNAG 5 cut(s) 190, 565, 690, 1335, 1473
BstF5I GGATG 5 cut(s) 208, 403, 520, 821, 1304
BstFNI CGCG 1 cut(s) 471
BstHHI GCGC 3 cut(s) 183, 471, 473
BstKTI GATC 5 cut(s) 146, 256, 333, 454, 1511
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 5 cut(s) 143, 253, 330, 451, 1508
BstMWI GCNNNNNNNGC 5 cut(s) 80, 560, 569, 575, 1100
BstNI CCWGG 2 cut(s) 227, 1002
BstSCI CCNGG 3 cut(s) 225, 523, 1000
BstSFI CTRYAG 3 cut(s) 549, 965, 1478
BstSLI GKGCMC 2 cut(s) 383, 1366
BstUI CGCG 1 cut(s) 471
BstV1I GCAGC 7 cut(s) 30, 86, 541, 556, 563, 716, 1081
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BsuI GTATCC 1 cut(s) 1242
BsuRI GGCC 5 cut(s) 207, 368, 563, 935, 1464
BtsCI GGATG 5 cut(s) 208, 403, 520, 821, 1304
BtsI GCAGTG 2 cut(s) 78, 190
BtsIMutI CAGTG 7 cut(s) 78, 190, 294, 381, 917, 1332, 1446
Cac8I GCNNGC 1 cut(s) 471
CaiI CAGNNNCTG 1 cut(s) 80
CciI TCATGA 1 cut(s) 9
CfoI GCGC 3 cut(s) 183, 471, 473
Cfr13I GGNCC 5 cut(s) 223, 366, 1144, 1376, 1451
CsiI ACCWGGT 1 cut(s) 225
Csp6I GTAC 1 cut(s) 1244
CviAII CATG 8 cut(s) 10, 257, 303, 481, 623, 736, 1241, 1286
CviQI GTAC 1 cut(s) 1244
DdeI CTNAG 5 cut(s) 190, 565, 690, 1335, 1473
DpnI GATC 5 cut(s) 145, 255, 332, 453, 1510
DpnII GATC 5 cut(s) 143, 253, 330, 451, 1508
DraI TTTAAA 1 cut(s) 1488
Eam1104I CTCTTC 3 cut(s) 601, 889, 1472
EarI CTCTTC 3 cut(s) 601, 889, 1472
Eco130I CCWWGG 1 cut(s) 558
Eco147I AGGCCT 1 cut(s) 1464
Eco47I GGWCC 4 cut(s) 223, 1144, 1376, 1451
Eco57I CTGAAG 1 cut(s) 69
EcoO109I RGGNCCY 2 cut(s) 1144, 1376
EcoRII CCWGG 2 cut(s) 225, 1000
EcoT14I CCWWGG 1 cut(s) 558
ErhI CCWWGG 1 cut(s) 558
FaeI CATG 8 cut(s) 13, 260, 306, 484, 626, 739, 1244, 1289
FalI AAGNNNNNCTT 2 cut(s) 484, 516
FaqI GGGAC 4 cut(s) 441, 726, 1437, 1438
FatI CATG 8 cut(s) 9, 256, 302, 480, 622, 735, 1240, 1285
FbaI TGATCA 1 cut(s) 451
Fnu4HI GCNGC 8 cut(s) 19, 75, 552, 555, 570, 730, 733, 1095
FokI GGATG 5 cut(s) 215, 390, 507, 828, 1311
Fsp4HI GCNGC 8 cut(s) 19, 75, 552, 555, 570, 730, 733, 1095
FspBI CTAG 8 cut(s) 35, 44, 209, 350, 617, 1067, 1457, 1615
GlaI GCGC 3 cut(s) 182, 470, 472
GluI GCNGC 8 cut(s) 19, 75, 552, 555, 570, 730, 733, 1095
HaeIII GGCC 5 cut(s) 207, 368, 563, 935, 1464
HapII CCGG 3 cut(s) 525, 1118, 1235
HhaI GCGC 3 cut(s) 183, 471, 473
Hin1II CATG 8 cut(s) 13, 260, 306, 484, 626, 739, 1244, 1289
Hin6I GCGC 3 cut(s) 181, 469, 471
HinP1I GCGC 3 cut(s) 181, 469, 471
HincII GTYRAC 1 cut(s) 1171
HindII GTYRAC 1 cut(s) 1171
HinfI GANTC 4 cut(s) 171, 188, 654, 674
HpaII CCGG 3 cut(s) 525, 1118, 1235
HphI GGTGA 4 cut(s) 554, 1319, 1503, 1538
Hpy166II GTNNAC 6 cut(s) 152, 223, 787, 1075, 1171, 1246
Hpy188I TCNGA 8 cut(s) 88, 357, 451, 532, 659, 1081, 1402, 1580
Hpy188III TCNNGA 7 cut(s) 10, 192, 322, 711, 1118, 1139, 1379
Hpy8I GTNNAC 6 cut(s) 152, 223, 787, 1075, 1171, 1246
HpyAV CCTTC 6 cut(s) 319, 878, 1115, 1278, 1415, 1611
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 1 cut(s) 1017
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 560, 569, 575, 1100
HpyF3I CTNAG 5 cut(s) 190, 565, 690, 1335, 1473
HpySE526I ACGT 1 cut(s) 1017
Hsp92II CATG 8 cut(s) 13, 260, 306, 484, 626, 739, 1244, 1289
HspAI GCGC 3 cut(s) 181, 469, 471
Kpn2I TCCGGA 1 cut(s) 1117
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 5 cut(s) 143, 253, 330, 451, 1508
LmnI GCTCC 5 cut(s) 15, 308, 466, 1223, 1415
Lsp1109I GCAGC 7 cut(s) 30, 86, 541, 556, 563, 716, 1081
LweI GCATC 3 cut(s) 235, 676, 1246
MabI ACCWGGT 1 cut(s) 225
MaeI CTAG 8 cut(s) 35, 44, 209, 350, 617, 1067, 1457, 1615
MaeII ACGT 1 cut(s) 1017
MaeIII GTNAC 4 cut(s) 185, 289, 1045, 1161
MalI GATC 5 cut(s) 145, 255, 332, 453, 1510
MbiI CCGCTC 1 cut(s) 1410
MboI GATC 5 cut(s) 143, 253, 330, 451, 1508
MboII GAAGA 6 cut(s) 588, 695, 906, 1055, 1274, 1459
MfeI CAATTG 1 cut(s) 1111
MflI RGATCY 1 cut(s) 143
MhlI GDGCHC 2 cut(s) 383, 1366
MluCI AATT 7 cut(s) 27, 263, 507, 1111, 1128, 1437, 1596
MlyI GAGTC 2 cut(s) 180, 182
MmeI TCCRAC 4 cut(s) 211, 1276, 1351, 1437
MroI TCCGGA 1 cut(s) 1117
MseI TTAA 2 cut(s) 93, 1487
MslI CAYNNNNRTG 1 cut(s) 1254
MspA1I CMGCKG 2 cut(s) 554, 569
MspI CCGG 3 cut(s) 525, 1118, 1235
MspR9I CCNGG 3 cut(s) 227, 525, 1002
MunI CAATTG 1 cut(s) 1111
Mva1269I GAATGC 1 cut(s) 281
MvaI CCWGG 2 cut(s) 227, 1002
MvnI CGCG 1 cut(s) 471
MwoI GCNNNNNNNGC 5 cut(s) 80, 560, 569, 575, 1100
NciI CCSGG 1 cut(s) 525
NdeII GATC 5 cut(s) 143, 253, 330, 451, 1508
NlaIII CATG 8 cut(s) 13, 260, 306, 484, 626, 739, 1244, 1289
NlaIV GGNNCC 1 cut(s) 1453
NmuCI GTSAC 3 cut(s) 185, 289, 1161
PagI TCATGA 1 cut(s) 9
PauI GCGCGC 1 cut(s) 469
PceI AGGCCT 1 cut(s) 1464
PctI GAATGC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 654, 674
PflMI CCANNNNNTGG 1 cut(s) 375
PkrI GCNGC 8 cut(s) 20, 76, 553, 556, 571, 731, 734, 1096
PleI GAGTC 2 cut(s) 179, 182
PpsI GAGTC 2 cut(s) 179, 182
Ppu21I YACGTR 1 cut(s) 1018
PpuMI RGGWCCY 2 cut(s) 1144, 1376
Psp5II RGGWCCY 2 cut(s) 1144, 1376
Psp6I CCWGG 2 cut(s) 225, 1000
PspGI CCWGG 2 cut(s) 225, 1000
PspN4I GGNNCC 1 cut(s) 1453
PspPI GGNCC 5 cut(s) 223, 366, 1144, 1376, 1451
PspPPI RGGWCCY 2 cut(s) 1144, 1376
PstI CTGCAG 2 cut(s) 553, 969
PstNI CAGNNNCTG 1 cut(s) 80
PsuI RGATCY 1 cut(s) 143
PteI GCGCGC 1 cut(s) 469
PvuII CAGCTG 2 cut(s) 554, 569
RsaI GTAC 1 cut(s) 1245
RsaNI GTAC 1 cut(s) 1244
RseI CAYNNNNRTG 1 cut(s) 1254
SaqAI TTAA 2 cut(s) 93, 1487
SatI GCNGC 8 cut(s) 19, 75, 552, 555, 570, 730, 733, 1095
Sau3AI GATC 5 cut(s) 143, 253, 330, 451, 1508
Sau96I GGNCC 5 cut(s) 223, 366, 1144, 1376, 1451
SchI GAGTC 2 cut(s) 180, 182
ScrFI CCNGG 3 cut(s) 227, 525, 1002
SduI GDGCHC 2 cut(s) 383, 1366
SexAI ACCWGGT 1 cut(s) 225
SfaNI GCATC 3 cut(s) 235, 676, 1246
SfcI CTRYAG 3 cut(s) 549, 965, 1478
SinI GGWCC 4 cut(s) 223, 1144, 1376, 1451
SmiMI CAYNNNNRTG 1 cut(s) 1254
SmlI CTYRAG 4 cut(s) 197, 213, 773, 1137
SmoI CTYRAG 4 cut(s) 197, 213, 773, 1137
SpeI ACTAGT 1 cut(s) 34
Sse9I AATT 7 cut(s) 27, 263, 507, 1111, 1128, 1437, 1596
SseBI AGGCCT 1 cut(s) 1464
SsiI CCGC 3 cut(s) 732, 1330, 1408
SspMI CTAG 8 cut(s) 35, 44, 209, 350, 617, 1067, 1457, 1615
StuI AGGCCT 1 cut(s) 1464
StyD4I CCNGG 3 cut(s) 225, 523, 1000
StyI CCWWGG 1 cut(s) 558
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 1 cut(s) 1020
TasI AATT 7 cut(s) 27, 263, 507, 1111, 1128, 1437, 1596
TatI WGTACW 1 cut(s) 1243
TauI GCSGC 1 cut(s) 735
TfiI GAWTC 2 cut(s) 654, 674
Tru1I TTAA 2 cut(s) 93, 1487
Tru9I TTAA 2 cut(s) 93, 1487
TscAI CASTG 7 cut(s) 85, 190, 294, 388, 924, 1339, 1453
TseFI GTSAC 3 cut(s) 185, 289, 1161
TseI GCWGC 7 cut(s) 18, 74, 551, 554, 569, 729, 1094
Tsp45I GTSAC 3 cut(s) 185, 289, 1161
TspDTI ATGAA 8 cut(s) 291, 382, 528, 611, 687, 1229, 1274, 1434
TspRI CASTG 7 cut(s) 85, 190, 294, 388, 924, 1339, 1453
Van91I CCANNNNNTGG 1 cut(s) 375
VpaK11BI GGWCC 4 cut(s) 223, 1144, 1376, 1451
XapI RAATTY 3 cut(s) 263, 1128, 1596
XcmI CCANNNNNNNNNTGG 1 cut(s) 998
XspI CTAG 8 cut(s) 35, 44, 209, 350, 617, 1067, 1457, 1615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.