MD16G1003200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010399
Physical Location & Seq
Forward (+)
193897 .. 195351
1455 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1003200.v1.1.491

Sequence Viewer

Length: 909 bp
ATGTTTAATAGGAAGCCAAGGCAACTGCGGTTTGAGGCAGATATGAATCGCCTCTTCCTATATACCAGCTACAATCGCTTGGGAAGGGATGCGGATGAGGCAGATGTGGACAAGATCATTGACATGGCTAATAAAGCCTCCATTGCTGATCAACAGATACAAGTCCAAGAAAACATTCATCTCCAAATTAAAAACTTCTGCGTGTCTATGGATGAAGTTCTTCTCCCTAATGTTAAGAAGATAAATGAGGGAACCGAATCACCTAAACAATCAAATGCTGCTACTCGACGAAGTGCTTTTAGTTTTGCTATTGGTAGGAGTGGTCCACCATACAGAGGTGCCCGAGAAAGGCCATTAGAGCGCACTGATGTTTCTCAAAAGTTGTATGATGGAACTGTGATCAATGCTCAACCTTGGGGTTATGGGGGTGAAACCCGTGACTTTTGGGATGGTTTAACTGTACCAGAAACCAGGCCTAGTAAGCAGCGAGATGAGAAAGGTCGAGATCGGATCTGGAGACTCCTCAGTAAGCCTTTGGATCAGAGGAAATTGGGAAAAAGTGGCGACGTAATAGAGCGGAGGAACCCATTAGCTTTGGCTCATTTTGCAACCATCCGGCAAAGGATATGTCTCCAAATGTTATGGGTCTATATTCCAAATGTAATATTTGGAGATGCAGAAGAAGTGAAGAGGAAGAGATTTGGAAGCTTTTGGTTCAAGCTTGGAGGTTCAAAAAATAGCGGCTCAGATGTTCCTGTTTTGAAGAGTCTTGTCCTGGTGGCTACAAGGGCACTTTGTGATGAAGTGGTGCTTCTGAACTATACGTTGAGCAACTCTAACGTCGGCCAGAGTGGTATACTCCTGTTGATGAGGAGGAAGACCGAAGGCGGTGGAGCTAAGTTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

303

Amino Acids

34.3

Weight (kDa)

9.73

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 338
AccBSI CCGCTC 1 cut(s) 577
AccI GTMKAC 1 cut(s) 856
AciI CCGC 5 cut(s) 28, 92, 577, 741, 888
AclWI GGATC 2 cut(s) 518, 546
AcoI YGGCCR 1 cut(s) 844
AdeI CACNNNGTG 1 cut(s) 797
AfaI GTAC 1 cut(s) 462
AfiI CCNNNNNNNGG 2 cut(s) 335, 348
AgsI TTSAA 3 cut(s) 718, 732, 763
AjnI CCWGG 2 cut(s) 470, 774
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
AluBI AGCT 5 cut(s) 69, 593, 708, 721, 896
AluI AGCT 5 cut(s) 69, 593, 708, 721, 896
Alw26I GTCTC 2 cut(s) 511, 635
AlwI GGATC 2 cut(s) 518, 546
Ama87I CYCGRG 1 cut(s) 342
AoxI GGCC 3 cut(s) 350, 473, 844
ApeKI GCWGC 2 cut(s) 278, 484
ArsI GACNNNNNNTTYG 2 cut(s) 613, 645
Asp700I GAANNNNTTC 2 cut(s) 174, 219
AspLEI GCGC 1 cut(s) 363
AspS9I GGNCC 1 cut(s) 323
AsuHPI GGTGA 2 cut(s) 252, 440
AvaI CYCGRG 1 cut(s) 342
AvaII GGWCC 1 cut(s) 323
BaeGI GKGCMC 2 cut(s) 343, 793
BanI GGYRCC 1 cut(s) 338
BbsI GAAGAC 1 cut(s) 884
BbvI GCAGC 2 cut(s) 265, 496
BccI CCATC 3 cut(s) 383, 443, 620
BciT130I CCWGG 2 cut(s) 472, 776
BclI TGATCA 2 cut(s) 148, 399
BcoDI GTCTC 2 cut(s) 511, 635
BfaI CTAG 1 cut(s) 477
BisI GCNGC 3 cut(s) 279, 485, 742
BlsI GCNGC 3 cut(s) 280, 486, 743
Bme1390I CCNGG 2 cut(s) 472, 776
Bme18I GGWCC 1 cut(s) 323
BmeT110I CYCGRG 1 cut(s) 342
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 3 cut(s) 253, 340, 584
BmrFI CCNGG 2 cut(s) 472, 776
BmsI GCATC 2 cut(s) 79, 664
BpiI GAAGAC 1 cut(s) 884
BpmI CTGGAG 1 cut(s) 535
BsaBI GATNNNNATC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 17, 413
Bsc4I CCNNNNNNNGG 2 cut(s) 335, 348
Bse3DI GCAATG 1 cut(s) 141
Bse8I GATNNNNATC 1 cut(s) 45
BseBI CCWGG 2 cut(s) 472, 776
BseDI CCNNGG 2 cut(s) 17, 413
BseGI GGATG 5 cut(s) 94, 100, 217, 454, 612
BseJI GATNNNNATC 1 cut(s) 45
BseLI CCNNNNNNNGG 2 cut(s) 335, 348
BseMI GCAATG 1 cut(s) 141
BseMII CTCAG 2 cut(s) 538, 759
BseRI GAGGAG 2 cut(s) 512, 886
BseSI GKGCMC 2 cut(s) 343, 793
BseXI GCAGC 2 cut(s) 265, 496
BshFI GGCC 3 cut(s) 352, 475, 846
BshNI GGYRCC 1 cut(s) 338
BsiHKCI CYCGRG 1 cut(s) 342
BsiSI CCGG 1 cut(s) 616
BslI CCNNNNNNNGG 2 cut(s) 335, 348
BsmAI GTCTC 2 cut(s) 511, 635
BsnI GGCC 3 cut(s) 352, 475, 846
BsoBI CYCGRG 1 cut(s) 342
Bsp1286I GDGCHC 2 cut(s) 343, 793
Bsp143I GATC 6 cut(s) 114, 148, 399, 505, 510, 538
BspACI CCGC 5 cut(s) 28, 92, 577, 741, 888
BspANI GGCC 3 cut(s) 352, 475, 846
BspCNI CTCAG 2 cut(s) 537, 758
BspLI GGNNCC 3 cut(s) 253, 340, 584
BspPI GGATC 2 cut(s) 518, 546
BspT107I GGYRCC 1 cut(s) 338
BsrBI CCGCTC 1 cut(s) 577
BsrDI GCAATG 1 cut(s) 141
BssECI CCNNGG 2 cut(s) 17, 413
BssMI GATC 6 cut(s) 114, 148, 399, 505, 510, 538
BssNAI GTATAC 1 cut(s) 857
BssT1I CCWWGG 2 cut(s) 17, 413
Bst1107I GTATAC 1 cut(s) 857
Bst2UI CCWGG 2 cut(s) 472, 776
Bst4CI ACNGT 2 cut(s) 397, 460
Bst6I CTCTTC 4 cut(s) 59, 683, 689, 758
BstDEI CTNAG 3 cut(s) 524, 745, 897
BstF5I GGATG 5 cut(s) 94, 100, 217, 454, 612
BstHHI GCGC 1 cut(s) 363
BstKTI GATC 6 cut(s) 117, 151, 402, 508, 513, 541
BstMAI GTCTC 2 cut(s) 511, 635
BstMBI GATC 6 cut(s) 114, 148, 399, 505, 510, 538
BstMWI GCNNNNNNNGC 8 cut(s) 75, 98, 134, 143, 358, 481, 605, 788
BstNI CCWGG 2 cut(s) 472, 776
BstSCI CCNGG 2 cut(s) 470, 774
BstSLI GKGCMC 2 cut(s) 343, 793
BstV1I GCAGC 2 cut(s) 265, 496
BstV2I GAAGAC 1 cut(s) 884
BstX2I RGATCY 1 cut(s) 510
BstYI RGATCY 1 cut(s) 510
BstZ17I GTATAC 1 cut(s) 857
BsuRI GGCC 3 cut(s) 352, 475, 846
BtsCI GGATG 5 cut(s) 94, 100, 217, 454, 612
BtsIMutI CAGTG 1 cut(s) 363
CfoI GCGC 1 cut(s) 363
Cfr13I GGNCC 1 cut(s) 323
Csp6I GTAC 1 cut(s) 461
CviAII CATG 1 cut(s) 124
CviQI GTAC 1 cut(s) 461
DdeI CTNAG 3 cut(s) 524, 745, 897
DpnI GATC 6 cut(s) 116, 150, 401, 507, 512, 540
DpnII GATC 6 cut(s) 114, 148, 399, 505, 510, 538
DraIII CACNNNGTG 1 cut(s) 797
EaeI YGGCCR 1 cut(s) 844
Eam1104I CTCTTC 4 cut(s) 59, 683, 689, 758
EarI CTCTTC 4 cut(s) 59, 683, 689, 758
Eco130I CCWWGG 2 cut(s) 17, 413
Eco147I AGGCCT 1 cut(s) 475
Eco47I GGWCC 1 cut(s) 323
Eco88I CYCGRG 1 cut(s) 342
EcoRII CCWGG 2 cut(s) 470, 774
EcoT14I CCWWGG 2 cut(s) 17, 413
ErhI CCWWGG 2 cut(s) 17, 413
FaeI CATG 1 cut(s) 127
FalI AAGNNNNNCTT 2 cut(s) 795, 827
FatI CATG 1 cut(s) 123
FbaI TGATCA 2 cut(s) 148, 399
FblI GTMKAC 1 cut(s) 856
Fnu4HI GCNGC 3 cut(s) 279, 485, 742
FokI GGATG 5 cut(s) 101, 107, 224, 461, 599
Fsp4HI GCNGC 3 cut(s) 279, 485, 742
FspBI CTAG 1 cut(s) 477
GlaI GCGC 1 cut(s) 362
GluI GCNGC 3 cut(s) 279, 485, 742
GsuI CTGGAG 1 cut(s) 535
HaeIII GGCC 3 cut(s) 352, 475, 846
HapII CCGG 1 cut(s) 616
HhaI GCGC 1 cut(s) 363
Hin1II CATG 1 cut(s) 127
Hin6I GCGC 1 cut(s) 361
HinP1I GCGC 1 cut(s) 361
HindIII AAGCTT 2 cut(s) 706, 719
HinfI GANTC 4 cut(s) 46, 257, 519, 766
HpaII CCGG 1 cut(s) 616
HphI GGTGA 2 cut(s) 252, 440
Hpy166II GTNNAC 3 cut(s) 109, 326, 857
Hpy188I TCNGA 4 cut(s) 510, 543, 748, 816
Hpy188III TCNNGA 2 cut(s) 503, 514
Hpy8I GTNNAC 3 cut(s) 109, 326, 857
Hpy99I CGWCG 3 cut(s) 291, 569, 845
HpyAV CCTTC 2 cut(s) 78, 878
HpyCH4III ACNGT 2 cut(s) 397, 460
HpyCH4IV ACGT 3 cut(s) 567, 824, 840
HpyCH4V TGCA 2 cut(s) 608, 677
HpyF10VI GCNNNNNNNGC 8 cut(s) 75, 98, 134, 143, 358, 481, 605, 788
HpyF3I CTNAG 3 cut(s) 524, 745, 897
HpySE526I ACGT 3 cut(s) 567, 824, 840
Hsp92II CATG 1 cut(s) 127
HspAI GCGC 1 cut(s) 361
Ksp22I TGATCA 2 cut(s) 148, 399
Kzo9I GATC 6 cut(s) 114, 148, 399, 505, 510, 538
LmnI GCTCC 1 cut(s) 893
Lsp1109I GCAGC 2 cut(s) 265, 496
LweI GCATC 2 cut(s) 79, 664
MaeI CTAG 1 cut(s) 477
MaeII ACGT 3 cut(s) 567, 824, 840
MaeIII GTNAC 1 cut(s) 437
MalI GATC 6 cut(s) 116, 150, 401, 507, 512, 540
MbiI CCGCTC 1 cut(s) 577
MboI GATC 6 cut(s) 114, 148, 399, 505, 510, 538
MboII GAAGA 8 cut(s) 46, 212, 250, 692, 700, 706, 775, 889
MflI RGATCY 1 cut(s) 510
MhlI GDGCHC 2 cut(s) 343, 793
MluCI AATT 2 cut(s) 186, 548
MlyI GAGTC 2 cut(s) 513, 775
MroXI GAANNNNTTC 2 cut(s) 174, 219
MseI TTAA 5 cut(s) 6, 189, 234, 455, 907
MslI CAYNNNNRTG 1 cut(s) 122
MspI CCGG 1 cut(s) 616
MspR9I CCNGG 2 cut(s) 472, 776
MvaI CCWGG 2 cut(s) 472, 776
MwoI GCNNNNNNNGC 8 cut(s) 75, 98, 134, 143, 358, 481, 605, 788
NdeII GATC 6 cut(s) 114, 148, 399, 505, 510, 538
NlaIII CATG 1 cut(s) 127
NlaIV GGNNCC 3 cut(s) 253, 340, 584
NmuCI GTSAC 1 cut(s) 437
PceI AGGCCT 1 cut(s) 475
PdmI GAANNNNTTC 2 cut(s) 174, 219
PfeI GAWTC 2 cut(s) 46, 257
PkrI GCNGC 3 cut(s) 280, 486, 743
PleI GAGTC 2 cut(s) 513, 774
PpsI GAGTC 2 cut(s) 513, 774
Psp6I CCWGG 2 cut(s) 470, 774
PspGI CCWGG 2 cut(s) 470, 774
PspN4I GGNNCC 3 cut(s) 253, 340, 584
PspPI GGNCC 1 cut(s) 323
PsuI RGATCY 1 cut(s) 510
RsaI GTAC 1 cut(s) 462
RsaNI GTAC 1 cut(s) 461
RseI CAYNNNNRTG 1 cut(s) 122
SaqAI TTAA 5 cut(s) 6, 189, 234, 455, 907
SatI GCNGC 3 cut(s) 279, 485, 742
Sau3AI GATC 6 cut(s) 114, 148, 399, 505, 510, 538
Sau96I GGNCC 1 cut(s) 323
SchI GAGTC 2 cut(s) 513, 775
ScrFI CCNGG 2 cut(s) 472, 776
SduI GDGCHC 2 cut(s) 343, 793
SfaNI GCATC 2 cut(s) 79, 664
SinI GGWCC 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 122
Sse9I AATT 2 cut(s) 186, 548
SseBI AGGCCT 1 cut(s) 475
SsiI CCGC 5 cut(s) 28, 92, 577, 741, 888
SspI AATATT 1 cut(s) 666
SspMI CTAG 1 cut(s) 477
StuI AGGCCT 1 cut(s) 475
StyD4I CCNGG 2 cut(s) 470, 774
StyI CCWWGG 2 cut(s) 17, 413
TaaI ACNGT 2 cut(s) 397, 460
TaiI ACGT 3 cut(s) 570, 827, 843
TaqI TCGA 2 cut(s) 286, 502
TaqII GACCGA 1 cut(s) 896
TasI AATT 2 cut(s) 186, 548
TauI GCSGC 1 cut(s) 744
TfiI GAWTC 2 cut(s) 46, 257
Tru1I TTAA 5 cut(s) 6, 189, 234, 455, 907
Tru9I TTAA 5 cut(s) 6, 189, 234, 455, 907
TscAI CASTG 1 cut(s) 370
TseFI GTSAC 1 cut(s) 437
TseI GCWGC 2 cut(s) 278, 484
Tsp45I GTSAC 1 cut(s) 437
TspDTI ATGAA 4 cut(s) 59, 167, 228, 816
TspRI CASTG 1 cut(s) 370
VpaK11BI GGWCC 1 cut(s) 323
XmiI GTMKAC 1 cut(s) 856
XmnI GAANNNNTTC 2 cut(s) 174, 219
XspI CTAG 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.