MD16G1008700.v1.1

Domain of unknown function (DUF4516)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010944
Physical Location & Seq
Reverse (-)
648477 .. 649132
656 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1008700.v1.1.491

Sequence Viewer

Length: 204 bp
ATGAATCGGGAATTTGGGGGAGGGAGGCCGCCAACGGGCACTCCATCTCTGGCTTGGTCCTCCGTCGTCGTTGTGGCTTCTCTTCTCGCAGGGGCCTCCGTCGTCCACAATCTCTACAAGCCTAATCTAGTGTTCGACGAAATGATTCACTCACCTCGCTGTGCGTTTTGTACAGACGCTGCCTCCTGTGGACGAAATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4516

Protein Analysis

68

Amino Acids

7.06

Weight (kDa)

7.77

Isoelectric Point (pI)

44.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4516 PF14990 10 - 50 7.2e-15 Domain of unknown function (DUF4516)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 29
AcsI RAATTY 1 cut(s) 11
AfaI GTAC 1 cut(s) 172
AfiI CCNNNNNNNGG 1 cut(s) 35
AlwNI CAGNNNCTG 1 cut(s) 179
AoxI GGCC 2 cut(s) 26, 93
ApeKI GCWGC 1 cut(s) 179
ApoI RAATTY 1 cut(s) 11
ArsI GACNNNNNNTTYG 1 cut(s) 183
Asp700I GAANNNNTTC 1 cut(s) 144
AspS9I GGNCC 2 cut(s) 57, 93
AsuHPI GGTGA 1 cut(s) 144
AvaII GGWCC 1 cut(s) 57
BaeGI GKGCMC 1 cut(s) 41
BbvI GCAGC 1 cut(s) 166
BccI CCATC 1 cut(s) 52
BfaI CTAG 1 cut(s) 128
BisI GCNGC 2 cut(s) 29, 180
BlsI GCNGC 2 cut(s) 30, 181
Bme18I GGWCC 1 cut(s) 57
BmgT120I GGNCC 2 cut(s) 57, 93
BmiI GGNNCC 1 cut(s) 94
BsaXI ACNNNNNCTCC 4 cut(s) 25, 55, 167, 197
Bsc4I CCNNNNNNNGG 1 cut(s) 35
BseLI CCNNNNNNNGG 1 cut(s) 35
BseSI GKGCMC 1 cut(s) 41
BseXI GCAGC 1 cut(s) 166
BshFI GGCC 2 cut(s) 28, 95
BslI CCNNNNNNNGG 1 cut(s) 35
BsnI GGCC 2 cut(s) 28, 95
Bsp1286I GDGCHC 1 cut(s) 41
Bsp1407I TGTACA 1 cut(s) 170
BspACI CCGC 1 cut(s) 29
BspANI GGCC 2 cut(s) 28, 95
BspLI GGNNCC 1 cut(s) 94
BsrGI TGTACA 1 cut(s) 170
Bst6I CTCTTC 1 cut(s) 87
BstAUI TGTACA 1 cut(s) 170
BstSLI GKGCMC 1 cut(s) 41
BstV1I GCAGC 1 cut(s) 166
BsuRI GGCC 2 cut(s) 28, 95
CaiI CAGNNNCTG 1 cut(s) 179
Cfr13I GGNCC 2 cut(s) 57, 93
CseI GACGC 1 cut(s) 185
Csp6I GTAC 1 cut(s) 171
CviJI RGCY 5 cut(s) 28, 53, 77, 95, 121
CviKI_1 RGCY 5 cut(s) 28, 53, 77, 95, 121
CviQI GTAC 1 cut(s) 171
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Eco47I GGWCC 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 93
Fnu4HI GCNGC 2 cut(s) 29, 180
Fsp4HI GCNGC 2 cut(s) 29, 180
FspBI CTAG 1 cut(s) 128
GluI GCNGC 2 cut(s) 29, 180
HaeIII GGCC 2 cut(s) 28, 95
HgaI GACGC 1 cut(s) 185
HinfI GANTC 2 cut(s) 4, 145
HphI GGTGA 1 cut(s) 144
Hpy166II GTNNAC 2 cut(s) 106, 191
Hpy188III TCNNGA 1 cut(s) 8
Hpy8I GTNNAC 2 cut(s) 106, 191
Hpy99I CGWCG 4 cut(s) 68, 71, 104, 140
LpnPI CCDG 3 cut(s) 35, 75, 199
Lsp1109I GCAGC 1 cut(s) 166
MaeI CTAG 1 cut(s) 128
MboII GAAGA 1 cut(s) 74
MhlI GDGCHC 1 cut(s) 41
MluCI AATT 1 cut(s) 11
MnlI CCTC 6 cut(s) 14, 18, 70, 106, 165, 193
MroXI GAANNNNTTC 1 cut(s) 144
NlaIV GGNNCC 1 cut(s) 94
PdmI GAANNNNTTC 1 cut(s) 144
PfeI GAWTC 2 cut(s) 4, 145
PkrI GCNGC 2 cut(s) 30, 181
PspN4I GGNNCC 1 cut(s) 94
PspPI GGNCC 2 cut(s) 57, 93
PstNI CAGNNNCTG 1 cut(s) 179
RsaI GTAC 1 cut(s) 172
RsaNI GTAC 1 cut(s) 171
SatI GCNGC 2 cut(s) 29, 180
Sau96I GGNCC 2 cut(s) 57, 93
SduI GDGCHC 1 cut(s) 41
SetI ASST 1 cut(s) 157
SinI GGWCC 1 cut(s) 57
Sse9I AATT 1 cut(s) 11
SsiI CCGC 1 cut(s) 29
SspMI CTAG 1 cut(s) 128
TaqI TCGA 1 cut(s) 135
TasI AATT 1 cut(s) 11
TatI WGTACW 1 cut(s) 170
TauI GCSGC 1 cut(s) 31
TfiI GAWTC 2 cut(s) 4, 145
TseI GCWGC 1 cut(s) 179
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 52, 88
VpaK11BI GGWCC 1 cut(s) 57
XapI RAATTY 1 cut(s) 11
XcmI CCANNNNNNNNNTGG 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 144
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.