pycom13g00930

Domain of unknown function (DUF4516)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010944
Physical Location & Seq
Reverse (-)
596092 .. 596533
442 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g00930.1

Sequence Viewer

Length: 189 bp
ATGAATCGGAAATTTGGGGGAGGGAGGCCGCCGACGGGCACTCCGTCACTGGCTTGGTCCTCCGTCGTCGTTGTACTTTCTCTTCTCGCCGGGGCCTCCGTTGTCCACAATCTCTGCAAGCCTGATCTTACGTTGCCTCCTGTGGATGGCGTTACTGGAACCAACGGCAAGCAACCTGAGAAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4516

Protein Analysis

63

Amino Acids

6.38

Weight (kDa)

9.1

Isoelectric Point (pI)

29.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4516 PF14990 10 - 48 7.9e-16 Domain of unknown function (DUF4516)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 29
AcsI RAATTY 1 cut(s) 11
AfaI GTAC 1 cut(s) 75
AfiI CCNNNNNNNGG 2 cut(s) 35, 146
AoxI GGCC 2 cut(s) 26, 93
ApoI RAATTY 1 cut(s) 11
AspS9I GGNCC 2 cut(s) 57, 93
AsuC2I CCSGG 1 cut(s) 91
AvaII GGWCC 1 cut(s) 57
BaeGI GKGCMC 1 cut(s) 41
BarI GAAGNNNNNNTAC 2 cut(s) 66, 98
BccI CCATC 1 cut(s) 140
BceAI ACGGC 1 cut(s) 181
BcnI CCSGG 1 cut(s) 91
BisI GCNGC 1 cut(s) 29
BlsI GCNGC 1 cut(s) 30
Bme1390I CCNGG 1 cut(s) 91
Bme18I GGWCC 1 cut(s) 57
BmgT120I GGNCC 2 cut(s) 57, 93
BmiI GGNNCC 2 cut(s) 94, 160
BmrFI CCNGG 1 cut(s) 91
BpuMI CCSGG 1 cut(s) 91
BsaJI CCNNGG 1 cut(s) 90
BsaXI ACNNNNNCTCC 4 cut(s) 25, 55, 121, 151
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 146
Bse1I ACTGG 2 cut(s) 54, 160
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 1 cut(s) 151
BseLI CCNNNNNNNGG 2 cut(s) 35, 146
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 2 cut(s) 54, 160
BseSI GKGCMC 1 cut(s) 41
BshFI GGCC 2 cut(s) 28, 95
BsiSI CCGG 1 cut(s) 90
BslI CCNNNNNNNGG 2 cut(s) 35, 146
BsnI GGCC 2 cut(s) 28, 95
Bsp1286I GDGCHC 1 cut(s) 41
Bsp143I GATC 1 cut(s) 124
BspACI CCGC 1 cut(s) 29
BspANI GGCC 2 cut(s) 28, 95
BspCNI CTCAG 1 cut(s) 169
BspLI GGNNCC 2 cut(s) 94, 160
BsrI ACTGG 2 cut(s) 54, 160
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 1 cut(s) 124
Bst6I CTCTTC 1 cut(s) 87
BstC8I GCNNGC 2 cut(s) 119, 170
BstDEI CTNAG 1 cut(s) 177
BstF5I GGATG 1 cut(s) 151
BstKTI GATC 1 cut(s) 127
BstMBI GATC 1 cut(s) 124
BstSCI CCNGG 1 cut(s) 89
BstSLI GKGCMC 1 cut(s) 41
BsuRI GGCC 2 cut(s) 28, 95
BtsCI GGATG 1 cut(s) 151
BtsIMutI CAGTG 1 cut(s) 47
Cac8I GCNNGC 2 cut(s) 119, 170
Cfr13I GGNCC 2 cut(s) 57, 93
Csp6I GTAC 1 cut(s) 74
CviJI RGCY 4 cut(s) 28, 53, 95, 121
CviKI_1 RGCY 4 cut(s) 28, 53, 95, 121
CviQI GTAC 1 cut(s) 74
DdeI CTNAG 1 cut(s) 177
DpnI GATC 1 cut(s) 126
DpnII GATC 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Eco47I GGWCC 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 93
Fnu4HI GCNGC 1 cut(s) 29
FokI GGATG 1 cut(s) 158
Fsp4HI GCNGC 1 cut(s) 29
GluI GCNGC 1 cut(s) 29
HaeIII GGCC 2 cut(s) 28, 95
HapII CCGG 1 cut(s) 90
HinfI GANTC 1 cut(s) 4
HpaII CCGG 1 cut(s) 90
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 1 cut(s) 9
Hpy8I GTNNAC 1 cut(s) 106
Hpy99I CGWCG 3 cut(s) 37, 68, 71
HpyCH4IV ACGT 1 cut(s) 131
HpyCH4V TGCA 1 cut(s) 117
HpyF3I CTNAG 1 cut(s) 177
HpySE526I ACGT 1 cut(s) 131
Kzo9I GATC 1 cut(s) 124
LpnPI CCDG 5 cut(s) 35, 103, 135, 141, 153
MaeII ACGT 1 cut(s) 131
MaeIII GTNAC 2 cut(s) 45, 151
MalI GATC 1 cut(s) 126
MboI GATC 1 cut(s) 124
MboII GAAGA 1 cut(s) 74
MhlI GDGCHC 1 cut(s) 41
MluCI AATT 1 cut(s) 11
MnlI CCTC 5 cut(s) 14, 18, 70, 106, 147
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 1 cut(s) 91
NciI CCSGG 1 cut(s) 91
NdeII GATC 1 cut(s) 124
NlaIV GGNNCC 2 cut(s) 94, 160
NmuCI GTSAC 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 41
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 1 cut(s) 30
PspN4I GGNNCC 2 cut(s) 94, 160
PspPI GGNCC 2 cut(s) 57, 93
RsaI GTAC 1 cut(s) 75
RsaNI GTAC 1 cut(s) 74
SatI GCNGC 1 cut(s) 29
Sau3AI GATC 1 cut(s) 124
Sau96I GGNCC 2 cut(s) 57, 93
ScrFI CCNGG 1 cut(s) 91
SduI GDGCHC 1 cut(s) 41
SetI ASST 2 cut(s) 134, 178
SinI GGWCC 1 cut(s) 57
Sse9I AATT 1 cut(s) 11
SsiI CCGC 1 cut(s) 29
StyD4I CCNGG 1 cut(s) 89
TaiI ACGT 1 cut(s) 134
TasI AATT 1 cut(s) 11
TatI WGTACW 1 cut(s) 73
TauI GCSGC 1 cut(s) 31
TfiI GAWTC 1 cut(s) 4
TscAI CASTG 1 cut(s) 54
TseFI GTSAC 1 cut(s) 45
Tsp45I GTSAC 1 cut(s) 45
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 3 cut(s) 33, 52, 88
TspRI CASTG 1 cut(s) 54
VpaK11BI GGWCC 1 cut(s) 57
XapI RAATTY 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.