Rw4G036530

Domain of unknown function (DUF4516)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0010944
Physical Location & Seq
Forward (+)
63360620 .. 63361827
1208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G036530.1

Sequence Viewer

Length: 192 bp
ATGAATCAGAAGTTCGGTGGAGGGAAGCTGCCGACCGGCACTCCATCGCTGGCTTGGTCCAGCGTCGTCGTCGTCGTCTCACTTCTCGCCGGAGCCTCCGTCGTCCACAATCTCTACAAGCCCGATCTTACATTGCCTCCTGTCGATGGTGTTAATGTGACCAAGGAGAAGGAATCGGAGAAGAAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4516

Protein Analysis

63

Amino Acids

6.65

Weight (kDa)

8.06

Isoelectric Point (pI)

27.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4516 PF14990 10 - 48 1.7e-17 Domain of unknown function (DUF4516)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfiI CCNNNNNNNGG 1 cut(s) 146
AluBI AGCT 1 cut(s) 28
AluI AGCT 1 cut(s) 28
Alw26I GTCTC 1 cut(s) 82
ApeKI GCWGC 1 cut(s) 28
AspS9I GGNCC 1 cut(s) 57
AvaII GGWCC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 15
BccI CCATC 2 cut(s) 52, 140
BcoDI GTCTC 1 cut(s) 82
BisI GCNGC 1 cut(s) 29
BlsI GCNGC 1 cut(s) 30
Bme18I GGWCC 1 cut(s) 57
BmgT120I GGNCC 1 cut(s) 57
BmiI GGNNCC 1 cut(s) 94
BsaJI CCNNGG 1 cut(s) 162
BsaXI ACNNNNNCTCC 6 cut(s) 25, 55, 84, 114, 121, 151
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse118I RCCGGY 1 cut(s) 35
Bse3DI GCAATG 1 cut(s) 131
BseDI CCNNGG 1 cut(s) 162
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMI GCAATG 1 cut(s) 131
BseXI GCAGC 1 cut(s) 15
Bsh1285I CGRYCG 1 cut(s) 36
BsiEI CGRYCG 1 cut(s) 36
BsiSI CCGG 2 cut(s) 36, 90
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 1 cut(s) 82
BsmBI CGTCTC 1 cut(s) 82
Bsp143I GATC 1 cut(s) 124
BspLI GGNNCC 1 cut(s) 94
BsrDI GCAATG 1 cut(s) 131
BsrFI RCCGGY 1 cut(s) 35
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 1 cut(s) 124
BssT1I CCWWGG 1 cut(s) 162
BstC8I GCNNGC 1 cut(s) 51
BstKTI GATC 1 cut(s) 127
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 1 cut(s) 124
BstMCI CGRYCG 1 cut(s) 36
BstV1I GCAGC 1 cut(s) 15
BtgZI GCGATG 1 cut(s) 30
Cac8I GCNNGC 1 cut(s) 51
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 57
CseI GACGC 1 cut(s) 52
CviJI RGCY 4 cut(s) 28, 53, 95, 121
CviKI_1 RGCY 4 cut(s) 28, 53, 95, 121
DpnI GATC 1 cut(s) 126
DpnII GATC 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 162
Eco47I GGWCC 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 162
Esp3I CGTCTC 1 cut(s) 82
Fnu4HI GCNGC 1 cut(s) 29
Fsp4HI GCNGC 1 cut(s) 29
GluI GCNGC 1 cut(s) 29
HapII CCGG 2 cut(s) 36, 90
HgaI GACGC 1 cut(s) 52
HinfI GANTC 2 cut(s) 4, 173
HpaII CCGG 2 cut(s) 36, 90
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 2 cut(s) 9, 178
Hpy8I GTNNAC 1 cut(s) 106
Hpy99I CGWCG 5 cut(s) 68, 71, 74, 77, 104
HpyAV CCTTC 1 cut(s) 163
Kzo9I GATC 1 cut(s) 124
LmnI GCTCC 1 cut(s) 92
LpnPI CCDG 5 cut(s) 35, 49, 73, 103, 153
Lsp1109I GCAGC 1 cut(s) 15
MaeIII GTNAC 1 cut(s) 157
MalI GATC 1 cut(s) 126
MboI GATC 1 cut(s) 124
MnlI CCTC 3 cut(s) 14, 106, 147
MseI TTAA 1 cut(s) 153
MspI CCGG 2 cut(s) 36, 90
NdeII GATC 1 cut(s) 124
NlaIV GGNNCC 1 cut(s) 94
NmuCI GTSAC 1 cut(s) 157
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PfeI GAWTC 2 cut(s) 4, 173
PkrI GCNGC 1 cut(s) 30
PspN4I GGNNCC 1 cut(s) 94
PspPI GGNCC 1 cut(s) 57
SaqAI TTAA 1 cut(s) 153
SatI GCNGC 1 cut(s) 29
Sau3AI GATC 1 cut(s) 124
Sau96I GGNCC 1 cut(s) 57
SetI ASST 1 cut(s) 30
SinI GGWCC 1 cut(s) 57
StyI CCWWGG 1 cut(s) 162
TaqI TCGA 1 cut(s) 144
TfiI GAWTC 2 cut(s) 4, 173
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 1 cut(s) 28
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 57
XcmI CCANNNNNNNNNTGG 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.