Rroxscaffold_5G00386300

Domain of unknown function (DUF4516)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010944
Physical Location & Seq
Forward (+)
65204372 .. 65205300
929 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386300.1

Sequence Viewer

Length: 417 bp
ATGATAGTCACCTTCCCCAATAACACTTCGTCTTGCCAGAAACCAAAGAAAACAGTCCGACAAATCCAACTCTTTCCGGCGAAGCAAACTATCTATTTGGAAGCCGGTTGGTTCTCTTTAGCTATTAATCAGAAGTTCGGCGGAGGGAAGCCGCCGACCGGCACTCCCTCGCTGTCTTGGTCCAGCGTCGTCGTCTCGATTCTCGCCGGAGCCACCGTCGTCCACAATCTCTACAAGCCCGATCTTGTGTTCGATGAAATGCCTGACTCAACTTGCAACACAGAATGTTCGATGAAATTTGTCGAGTCATTTATAATGAATGATGAGTTTATGTTTCGCTTTGTGTGCAGACATATATTGCCTCATGTCGATGGTGTTAATGTGACCAAGAAGAACCAACCGGAGAAGAAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4516

Protein Analysis

138

Amino Acids

15.5

Weight (kDa)

8.71

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4516 PF14990 52 - 85 7.4e-11 Domain of unknown function (DUF4516)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 314
AciI CCGC 2 cut(s) 141, 152
AcsI RAATTY 1 cut(s) 296
AfiI CCNNNNNNNGG 1 cut(s) 158
AluBI AGCT 1 cut(s) 122
AluI AGCT 1 cut(s) 122
Alw26I GTCTC 1 cut(s) 199
ApoI RAATTY 1 cut(s) 296
AseI ATTAAT 1 cut(s) 126
AspS9I GGNCC 1 cut(s) 180
AvaII GGWCC 1 cut(s) 180
BccI CCATC 1 cut(s) 365
BcoDI GTCTC 1 cut(s) 199
BisI GCNGC 1 cut(s) 152
BlsI GCNGC 1 cut(s) 153
Bme18I GGWCC 1 cut(s) 180
BmgT120I GGNCC 1 cut(s) 180
BmiI GGNNCC 1 cut(s) 211
BsaWI WCCGGW 1 cut(s) 400
BsaXI ACNNNNNCTCC 4 cut(s) 148, 178, 201, 231
Bsc4I CCNNNNNNNGG 1 cut(s) 158
Bse118I RCCGGY 2 cut(s) 104, 158
BseLI CCNNNNNNNGG 1 cut(s) 158
BsgI GTGCAG 1 cut(s) 367
Bsh1285I CGRYCG 1 cut(s) 159
BsiEI CGRYCG 1 cut(s) 159
BsiSI CCGG 5 cut(s) 77, 105, 159, 207, 401
BslI CCNNNNNNNGG 1 cut(s) 158
BsmAI GTCTC 1 cut(s) 199
BsmBI CGTCTC 1 cut(s) 199
Bsp143I GATC 1 cut(s) 241
BspACI CCGC 2 cut(s) 141, 152
BspLI GGNNCC 1 cut(s) 211
BsrFI RCCGGY 2 cut(s) 104, 158
BssAI RCCGGY 2 cut(s) 104, 158
BssMI GATC 1 cut(s) 241
Bst4CI ACNGT 2 cut(s) 55, 217
BstKTI GATC 1 cut(s) 244
BstMAI GTCTC 1 cut(s) 199
BstMBI GATC 1 cut(s) 241
BstMCI CGRYCG 1 cut(s) 159
BstMWI GCNNNNNNNGC 1 cut(s) 345
Cfr10I RCCGGY 2 cut(s) 104, 158
Cfr13I GGNCC 1 cut(s) 180
CseI GACGC 1 cut(s) 175
CviAII CATG 1 cut(s) 365
CviJI RGCY 5 cut(s) 104, 122, 151, 212, 238
CviKI_1 RGCY 5 cut(s) 104, 122, 151, 212, 238
DpnI GATC 1 cut(s) 243
DpnII GATC 1 cut(s) 241
EciI GGCGGA 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 180
Esp3I CGTCTC 1 cut(s) 199
FaeI CATG 1 cut(s) 368
FaiI YATR 5 cut(s) 314, 332, 354, 356, 366
FatI CATG 1 cut(s) 364
Fnu4HI GCNGC 1 cut(s) 152
Fsp4HI GCNGC 1 cut(s) 152
GluI GCNGC 1 cut(s) 152
HapII CCGG 5 cut(s) 77, 105, 159, 207, 401
HgaI GACGC 1 cut(s) 175
Hin1II CATG 1 cut(s) 368
HinfI GANTC 3 cut(s) 199, 266, 305
HpaII CCGG 5 cut(s) 77, 105, 159, 207, 401
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 2 cut(s) 59, 132
Hpy188III TCNNGA 1 cut(s) 196
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 3 cut(s) 191, 194, 221
HpyAV CCTTC 1 cut(s) 22
HpyCH4III ACNGT 2 cut(s) 55, 217
HpyCH4V TGCA 2 cut(s) 276, 348
HpyF10VI GCNNNNNNNGC 1 cut(s) 345
Hsp92II CATG 1 cut(s) 368
Kzo9I GATC 1 cut(s) 241
LmnI GCTCC 1 cut(s) 209
LpnPI CCDG 7 cut(s) 50, 90, 118, 172, 196, 220, 276
MaeIII GTNAC 2 cut(s) 7, 382
MalI GATC 1 cut(s) 243
MboI GATC 1 cut(s) 241
MboII GAAGA 1 cut(s) 403
MluCI AATT 1 cut(s) 296
MlyI GAGTC 2 cut(s) 260, 314
MmeI TCCRAC 2 cut(s) 82, 91
MnlI CCTC 3 cut(s) 137, 178, 372
MseI TTAA 2 cut(s) 126, 378
MslI CAYNNNNRTG 1 cut(s) 369
MspI CCGG 5 cut(s) 77, 105, 159, 207, 401
MwoI GCNNNNNNNGC 1 cut(s) 345
NdeII GATC 1 cut(s) 241
NlaIII CATG 1 cut(s) 368
NlaIV GGNNCC 1 cut(s) 211
NmuCI GTSAC 2 cut(s) 7, 382
PfeI GAWTC 1 cut(s) 199
PkrI GCNGC 1 cut(s) 153
PleI GAGTC 2 cut(s) 260, 313
PpsI GAGTC 2 cut(s) 260, 313
PshBI ATTAAT 1 cut(s) 126
PsiI TTATAA 1 cut(s) 314
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 1 cut(s) 180
RseI CAYNNNNRTG 1 cut(s) 369
SaqAI TTAA 2 cut(s) 126, 378
SatI GCNGC 1 cut(s) 152
Sau3AI GATC 1 cut(s) 241
Sau96I GGNCC 1 cut(s) 180
SchI GAGTC 2 cut(s) 260, 314
SetI ASST 2 cut(s) 14, 124
SinI GGWCC 1 cut(s) 180
SmiMI CAYNNNNRTG 1 cut(s) 369
Sse9I AATT 1 cut(s) 296
SsiI CCGC 2 cut(s) 141, 152
TaaI ACNGT 2 cut(s) 55, 217
TaqI TCGA 5 cut(s) 197, 252, 290, 303, 369
TasI AATT 1 cut(s) 296
TauI GCSGC 1 cut(s) 154
TfiI GAWTC 1 cut(s) 199
Tru1I TTAA 2 cut(s) 126, 378
Tru9I TTAA 2 cut(s) 126, 378
TseFI GTSAC 2 cut(s) 7, 382
Tsp45I GTSAC 2 cut(s) 7, 382
TspDTI ATGAA 3 cut(s) 270, 308, 332
VpaK11BI GGWCC 1 cut(s) 180
VspI ATTAAT 1 cut(s) 126
XapI RAATTY 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.