RchiOBHm_Chr4g0445941

Domain of unknown function (DUF4516)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0010944
Physical Location & Seq
Forward (+)
66261242 .. 66261538
297 bp
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UTR
Exon/CDS
Intron
PRQ41348

Sequence Viewer

Length: 297 bp
ATGACTTTGGAAAGCTCTGGTGTTGAACTTGGACTGAAAAGAGCTAGGAAGGCAGGTGAGGTTTTCGCCAAGTCTAGCCACGACATTGAGGTAGGAAACAGTGACAATAAAGCTATGAATCAGAAGTCGAGCGGAAGGAAGCTGCCGACTGGCACTCTCTCCCTGGCTTGGTCCGGAGTTGTGGTCGTCTCGCTTCTCGCCGGAGCCTTCGTCGTCCACAGTGTCTACAAGCTCGATCTTGTACTCTCTCTCTCTCTACATTATTATGCCTCTTTTGGTTTTACAATTTCATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4516

Protein Analysis

98

Amino Acids

10.44

Weight (kDa)

9.4

Isoelectric Point (pI)

28.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4516 PF14990 48 - 80 1.1e-06 Domain of unknown function (DUF4516)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 44
Acc36I ACCTGC 1 cut(s) 44
AccBSI CCGCTC 1 cut(s) 132
AccI GTMKAC 1 cut(s) 225
AccIII TCCGGA 1 cut(s) 173
AciI CCGC 1 cut(s) 132
AfaI GTAC 1 cut(s) 243
AfiI CCNNNNNNNGG 1 cut(s) 168
AgsI TTSAA 1 cut(s) 26
AjnI CCWGG 1 cut(s) 162
AluBI AGCT 5 cut(s) 15, 44, 113, 142, 232
AluI AGCT 5 cut(s) 15, 44, 113, 142, 232
Alw26I GTCTC 1 cut(s) 193
Aor13HI TCCGGA 1 cut(s) 173
ApeKI GCWGC 1 cut(s) 142
AspS9I GGNCC 1 cut(s) 171
AsuHPI GGTGA 1 cut(s) 68
AvaII GGWCC 1 cut(s) 171
BbvI GCAGC 1 cut(s) 129
BciT130I CCWGG 1 cut(s) 164
BcoDI GTCTC 1 cut(s) 193
BfaI CTAG 2 cut(s) 45, 75
BfuAI ACCTGC 1 cut(s) 44
BisI GCNGC 1 cut(s) 143
BlsI GCNGC 1 cut(s) 144
Bme1390I CCNGG 1 cut(s) 164
Bme18I GGWCC 1 cut(s) 171
BmgT120I GGNCC 1 cut(s) 171
BmiI GGNNCC 1 cut(s) 205
BmrFI CCNGG 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 162
BsaWI WCCGGW 1 cut(s) 173
BsaXI ACNNNNNCTCC 4 cut(s) 168, 195, 198, 225
Bsc4I CCNNNNNNNGG 1 cut(s) 168
Bse1I ACTGG 1 cut(s) 154
BseAI TCCGGA 1 cut(s) 173
BseBI CCWGG 1 cut(s) 164
BseDI CCNNGG 1 cut(s) 162
BseLI CCNNNNNNNGG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 154
BseXI GCAGC 1 cut(s) 129
BsiSI CCGG 2 cut(s) 174, 201
BslI CCNNNNNNNGG 1 cut(s) 168
BsmAI GTCTC 1 cut(s) 193
BsmBI CGTCTC 1 cut(s) 193
Bsp13I TCCGGA 1 cut(s) 173
Bsp143I GATC 1 cut(s) 235
BspACI CCGC 1 cut(s) 132
BspEI TCCGGA 1 cut(s) 173
BspLI GGNNCC 1 cut(s) 205
BspMI ACCTGC 1 cut(s) 44
BsrBI CCGCTC 1 cut(s) 132
BsrI ACTGG 1 cut(s) 154
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 1 cut(s) 235
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 2 cut(s) 101, 221
BstKTI GATC 1 cut(s) 238
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 1 cut(s) 235
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 1 cut(s) 164
BstSCI CCNGG 1 cut(s) 162
BstV1I GCAGC 1 cut(s) 129
BtsIMutI CAGTG 2 cut(s) 106, 226
BveI ACCTGC 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 171
Csp6I GTAC 1 cut(s) 242
CviJI RGCY 8 cut(s) 15, 44, 78, 113, 142, 167, 206, 232
CviKI_1 RGCY 8 cut(s) 15, 44, 78, 113, 142, 167, 206, 232
CviQI GTAC 1 cut(s) 242
DpnI GATC 1 cut(s) 237
DpnII GATC 1 cut(s) 235
Eco47I GGWCC 1 cut(s) 171
EcoRII CCWGG 1 cut(s) 162
Esp3I CGTCTC 1 cut(s) 193
FaiI YATR 3 cut(s) 116, 267, 295
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 1 cut(s) 143
Fsp4HI GCNGC 1 cut(s) 143
FspBI CTAG 2 cut(s) 45, 75
GluI GCNGC 1 cut(s) 143
HapII CCGG 2 cut(s) 174, 201
HinfI GANTC 1 cut(s) 118
HpaII CCGG 2 cut(s) 174, 201
HphI GGTGA 1 cut(s) 68
Hpy166II GTNNAC 2 cut(s) 217, 226
Hpy188I TCNGA 1 cut(s) 123
Hpy188III TCNNGA 1 cut(s) 174
Hpy8I GTNNAC 2 cut(s) 217, 226
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 3 cut(s) 43, 129, 217
HpyCH4III ACNGT 2 cut(s) 101, 221
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
Kpn2I TCCGGA 1 cut(s) 173
Kzo9I GATC 1 cut(s) 235
LmnI GCTCC 1 cut(s) 203
LpnPI CCDG 7 cut(s) 3, 39, 135, 149, 176, 187, 214
Lsp1109I GCAGC 1 cut(s) 129
MaeI CTAG 2 cut(s) 45, 75
MaeIII GTNAC 1 cut(s) 101
MalI GATC 1 cut(s) 237
MbiI CCGCTC 1 cut(s) 132
MboI GATC 1 cut(s) 235
MluCI AATT 1 cut(s) 285
MnlI CCTC 3 cut(s) 52, 82, 280
MroI TCCGGA 1 cut(s) 173
MslI CAYNNNNRTG 1 cut(s) 264
MspI CCGG 2 cut(s) 174, 201
MspR9I CCNGG 1 cut(s) 164
MvaI CCWGG 1 cut(s) 164
MwoI GCNNNNNNNGC 1 cut(s) 50
NdeII GATC 1 cut(s) 235
NlaIV GGNNCC 1 cut(s) 205
NmuCI GTSAC 1 cut(s) 101
PaqCI CACCTGC 1 cut(s) 44
PfeI GAWTC 1 cut(s) 118
PkrI GCNGC 1 cut(s) 144
Psp6I CCWGG 1 cut(s) 162
PspGI CCWGG 1 cut(s) 162
PspN4I GGNNCC 1 cut(s) 205
PspPI GGNCC 1 cut(s) 171
RsaI GTAC 1 cut(s) 243
RsaNI GTAC 1 cut(s) 242
RseI CAYNNNNRTG 1 cut(s) 264
SatI GCNGC 1 cut(s) 143
Sau3AI GATC 1 cut(s) 235
Sau96I GGNCC 1 cut(s) 171
ScrFI CCNGG 1 cut(s) 164
SetI ASST 8 cut(s) 17, 46, 58, 63, 93, 115, 144, 234
SinI GGWCC 1 cut(s) 171
SmiMI CAYNNNNRTG 1 cut(s) 264
Sse9I AATT 1 cut(s) 285
SsiI CCGC 1 cut(s) 132
SspMI CTAG 2 cut(s) 45, 75
StyD4I CCNGG 1 cut(s) 162
TaaI ACNGT 2 cut(s) 101, 221
TaqI TCGA 2 cut(s) 128, 234
TasI AATT 1 cut(s) 285
TatI WGTACW 1 cut(s) 241
TfiI GAWTC 1 cut(s) 118
TscAI CASTG 2 cut(s) 106, 226
TseFI GTSAC 1 cut(s) 101
TseI GCWGC 1 cut(s) 142
Tsp45I GTSAC 1 cut(s) 101
TspDTI ATGAA 2 cut(s) 131, 279
TspRI CASTG 2 cut(s) 106, 226
VpaK11BI GGWCC 1 cut(s) 171
XmiI GTMKAC 1 cut(s) 225
XspI CTAG 2 cut(s) 45, 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.