MD16G1106900.v1.1
ERF Family

S-adenosyl-L-methionine-dependent methyltransferases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014962
Physical Location & Seq
Forward (+)
7502498 .. 7503331
834 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1106900.v1.1.491

Sequence Viewer

Length: 834 bp
ATGAAACACAACATTCCAACTTTCAAATACTTGTTCCTCTCTCTCCTCCTCCTCACTCTTCCCATTCTCCTTCTCGTCCTCCGTCTCCAACCCCACAACCTCCACAGGACGGCCGCTACCGCCGTCGTCACTACACACAATAACATCCTCCCGGTTATACAACCCGAATCCCAACAAGAAACAGAAACCAATACCTCATCCGGCGAAGACATCCGAATCCGACCAGGCTACACCTCCTACGAAGCCTACCTCGAGCGGCAGGTCAACAAAACCAACAACCCTAGGCTCCGCCAGTTATGGACCACCCGCGACTGGGACCGAAAAATCCAAGTCTTCTCCCAAGTCTTCCAACAAATGAAGCGCCAGAACCTCCTCACCAACGGCTCCAAGGCCCTCTGCATCGGGGCTAGAGTCGGCCAGGAAGTTGAGGCGTTGAAGCGAGTCGGGGTGTCCGACTCCGTCGGCATCGACCTCGTACCGTTCCCTCCGCTCGTGGTCAAGGGCGACTTCCACAACCAGCCATTTGGCGACGACACCTTCGACTTCGAGTTCTCGAACGTGTTCGACCACGCTCTGTACCCGCGGAAGTTCGTTTCCGAGATCGAACGGACGTTGAGGCCCGGCGGGGTCTGCGTTTTACACGTGGCAATCTCGCGGCGGACGGATAAGTACTCGGCGAATGATCTGTATAGTGTGAAGCCGTTGGTGGCAATGTTTCGGAGGAGTAAGTTGGTTCATGTCAAGGAGGTTGACGGTTTTGGTGCTAACACGGAGGTGGTTTTTCGGAAACGGCAAAAGGGGAGCCCAGCCCATCCAACGGTCCCAGTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

278

Amino Acids

31.55

Weight (kDa)

9.61

Isoelectric Point (pI)

48.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 132 - 213 1.3e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014962)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24480 AT4G24805
fragaria_vesca FvH4_4g25070
malus_domestica MD13G1106800.v1.1 MD16G1106900.v1.1
prunus_persica Prupe.1G239500_v2.0.a1
pyrus_communis pycom13g09260
rosa_chinensis RchiOBHm_Chr4g0432251
rosa_laevigata RLG00000006859
rosa_multiflora Rmu_co8384597.1_g000001
rosa_roxburghii Rroxscaffold_5G00373700
rosa_rugosa Rorug04G0255600
rosa_samantha Rh4BG320200 Rh4CG335400 Rh4DG315600
rosa_wichuraiana Rw4G027180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 250
AccBSI CCGCTC 2 cut(s) 256, 490
AccII CGCG 3 cut(s) 309, 583, 655
AcoI YGGCCR 2 cut(s) 111, 415
AcvI CACGTG 1 cut(s) 643
AfaI GTAC 3 cut(s) 477, 578, 671
AfiI CCNNNNNNNGG 4 cut(s) 109, 312, 313, 817
AflIII ACRYGT 2 cut(s) 558, 640
AgsI TTSAA 2 cut(s) 25, 436
AjnI CCWGG 2 cut(s) 223, 417
AleI CACNNNNGTG 1 cut(s) 773
Alw26I GTCTC 1 cut(s) 89
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 4 cut(s) 111, 390, 415, 617
ArsI GACNNNNNNTTYG 2 cut(s) 521, 553
Asp700I GAANNNNTTC 1 cut(s) 560
AspA2I CCTAGG 1 cut(s) 281
AspLEI GCGC 1 cut(s) 363
AspS9I GGNCC 5 cut(s) 300, 316, 391, 618, 820
AsuC2I CCSGG 2 cut(s) 152, 621
AsuHPI GGTGA 1 cut(s) 367
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 3 cut(s) 300, 316, 820
AvrII CCTAGG 1 cut(s) 281
BanII GRGCYC 1 cut(s) 806
BauI CACGAG 1 cut(s) 491
BbrPI CACGTG 1 cut(s) 643
BbsI GAAGAC 3 cut(s) 213, 325, 337
BccI CCATC 1 cut(s) 819
BceAI ACGGC 5 cut(s) 107, 126, 397, 685, 806
BcgI CGANNNNNNTGC 2 cut(s) 454, 488
BciT130I CCWGG 2 cut(s) 225, 419
BcnI CCSGG 2 cut(s) 152, 621
BcoDI GTCTC 1 cut(s) 89
BfaI CTAG 2 cut(s) 282, 408
BfoI RGCGCY 1 cut(s) 364
BfuAI ACCTGC 1 cut(s) 250
BisI GCNGC 3 cut(s) 114, 257, 656
BlnI CCTAGG 1 cut(s) 281
BlsI GCNGC 3 cut(s) 115, 258, 657
BmcAI AGTACT 1 cut(s) 671
Bme1390I CCNGG 4 cut(s) 152, 225, 419, 621
Bme18I GGWCC 3 cut(s) 300, 316, 820
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 5 cut(s) 300, 316, 391, 618, 820
BmiI GGNNCC 5 cut(s) 287, 317, 385, 803, 822
BmrFI CCNGG 4 cut(s) 152, 225, 419, 621
BmrI ACTGGG 2 cut(s) 322, 818
BmsI GCATC 2 cut(s) 408, 474
BmuI ACTGGG 2 cut(s) 322, 818
BpiI GAAGAC 3 cut(s) 213, 325, 337
BpuMI CCSGG 2 cut(s) 152, 621
BsaAI YACGTR 1 cut(s) 643
BsaJI CCNNGG 3 cut(s) 281, 387, 581
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 312, 313, 817
Bse1I ACTGG 3 cut(s) 292, 317, 824
Bse3DI GCAATG 1 cut(s) 717
BseBI CCWGG 2 cut(s) 225, 419
BseDI CCNNGG 3 cut(s) 281, 387, 581
BseGI GGATG 4 cut(s) 144, 197, 210, 811
BseLI CCNNNNNNNGG 4 cut(s) 109, 312, 313, 817
BseMI GCAATG 1 cut(s) 717
BseNI ACTGG 3 cut(s) 292, 317, 824
BseRI GAGGAG 5 cut(s) 35, 38, 41, 362, 736
BseX3I CGGCCG 1 cut(s) 111
BseYI CCCAGC 1 cut(s) 805
Bsh1236I CGCG 3 cut(s) 309, 583, 655
Bsh1285I CGRYCG 1 cut(s) 114
BshFI GGCC 4 cut(s) 113, 392, 417, 619
BsiEI CGRYCG 1 cut(s) 114
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 3 cut(s) 152, 201, 621
BslFI GGGAC 2 cut(s) 329, 806
BslI CCNNNNNNNGG 4 cut(s) 109, 312, 313, 817
BsmAI GTCTC 1 cut(s) 89
BsmBI CGTCTC 1 cut(s) 89
BsmFI GGGAC 2 cut(s) 329, 806
BsnI GGCC 4 cut(s) 113, 392, 417, 619
BsoBI CYCGRG 1 cut(s) 251
Bsp1286I GDGCHC 1 cut(s) 806
Bsp143I GATC 2 cut(s) 600, 682
BspANI GGCC 4 cut(s) 113, 392, 417, 619
BspFNI CGCG 3 cut(s) 309, 583, 655
BspLI GGNNCC 5 cut(s) 287, 317, 385, 803, 822
BspMI ACCTGC 1 cut(s) 250
BsrBI CCGCTC 2 cut(s) 256, 490
BsrDI GCAATG 1 cut(s) 717
BsrI ACTGG 3 cut(s) 292, 317, 824
BssECI CCNNGG 3 cut(s) 281, 387, 581
BssMI GATC 2 cut(s) 600, 682
BssSI CACGAG 1 cut(s) 491
BssT1I CCWWGG 2 cut(s) 281, 387
Bst2BI CACGAG 1 cut(s) 491
Bst2UI CCWGG 2 cut(s) 225, 419
Bst4CI ACNGT 3 cut(s) 480, 755, 820
Bst6I CTCTTC 1 cut(s) 63
BstBAI YACGTR 1 cut(s) 643
BstDSI CCRYGG 1 cut(s) 581
BstF5I GGATG 4 cut(s) 144, 197, 210, 811
BstFNI CGCG 3 cut(s) 309, 583, 655
BstH2I RGCGCY 1 cut(s) 364
BstHHI GCGC 1 cut(s) 363
BstKTI GATC 2 cut(s) 603, 685
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 2 cut(s) 600, 682
BstMCI CGRYCG 1 cut(s) 114
BstMWI GCNNNNNNNGC 2 cut(s) 119, 630
BstNI CCWGG 2 cut(s) 225, 419
BstSCI CCNGG 4 cut(s) 150, 223, 417, 619
BstUI CGCG 3 cut(s) 309, 583, 655
BstV2I GAAGAC 3 cut(s) 213, 325, 337
BstXI CCANNNNNNTGG 1 cut(s) 524
BstZI CGGCCG 1 cut(s) 111
BsuRI GGCC 4 cut(s) 113, 392, 417, 619
BtgI CCRYGG 1 cut(s) 581
BtsCI GGATG 4 cut(s) 144, 197, 210, 811
BveI ACCTGC 1 cut(s) 250
CfoI GCGC 1 cut(s) 363
Cfr13I GGNCC 5 cut(s) 300, 316, 391, 618, 820
Cfr42I CCGCGG 1 cut(s) 584
Csp6I GTAC 3 cut(s) 476, 577, 670
CviAII CATG 1 cut(s) 737
CviQI GTAC 3 cut(s) 476, 577, 670
DpnI GATC 2 cut(s) 602, 684
DpnII GATC 2 cut(s) 600, 682
EaeI YGGCCR 2 cut(s) 111, 415
EagI CGGCCG 1 cut(s) 111
Eam1104I CTCTTC 1 cut(s) 63
EarI CTCTTC 1 cut(s) 63
EciI GGCGGA 2 cut(s) 278, 673
EclXI CGGCCG 1 cut(s) 111
Eco130I CCWWGG 2 cut(s) 281, 387
Eco24I GRGCYC 1 cut(s) 806
Eco47I GGWCC 3 cut(s) 300, 316, 820
Eco52I CGGCCG 1 cut(s) 111
Eco72I CACGTG 1 cut(s) 643
Eco88I CYCGRG 1 cut(s) 251
EcoO109I RGGNCCY 1 cut(s) 391
EcoRII CCWGG 2 cut(s) 223, 417
EcoT14I CCWWGG 2 cut(s) 281, 387
EcoT38I GRGCYC 1 cut(s) 806
ErhI CCWWGG 2 cut(s) 281, 387
Esp3I CGTCTC 1 cut(s) 89
FaeI CATG 1 cut(s) 740
FaiI YATR 4 cut(s) 158, 298, 690, 738
FalI AAGNNNNNCTT 2 cut(s) 491, 523
FaqI GGGAC 2 cut(s) 329, 806
FatI CATG 1 cut(s) 736
FauI CCCGC 3 cut(s) 314, 588, 617
Fnu4HI GCNGC 3 cut(s) 114, 257, 656
FokI GGATG 4 cut(s) 131, 184, 197, 798
FriOI GRGCYC 1 cut(s) 806
Fsp4HI GCNGC 3 cut(s) 114, 257, 656
FspBI CTAG 2 cut(s) 282, 408
GlaI GCGC 1 cut(s) 362
GluI GCNGC 3 cut(s) 114, 257, 656
GsaI CCCAGC 1 cut(s) 809
HaeII RGCGCY 1 cut(s) 364
HaeIII GGCC 4 cut(s) 113, 392, 417, 619
HapII CCGG 3 cut(s) 152, 201, 621
HhaI GCGC 1 cut(s) 363
Hin1II CATG 1 cut(s) 740
Hin6I GCGC 1 cut(s) 361
HinP1I GCGC 1 cut(s) 361
HincII GTYRAC 2 cut(s) 265, 751
HindII GTYRAC 2 cut(s) 265, 751
HinfI GANTC 5 cut(s) 167, 216, 411, 441, 455
HpaII CCGG 3 cut(s) 152, 201, 621
HphI GGTGA 1 cut(s) 367
Hpy166II GTNNAC 2 cut(s) 265, 751
Hpy188I TCNGA 6 cut(s) 215, 221, 454, 598, 720, 786
Hpy188III TCNNGA 1 cut(s) 553
Hpy8I GTNNAC 2 cut(s) 265, 751
Hpy99I CGWCG 3 cut(s) 128, 464, 533
HpyAV CCTTC 2 cut(s) 80, 547
HpyCH4III ACNGT 3 cut(s) 480, 755, 820
HpyCH4IV ACGT 3 cut(s) 558, 611, 642
HpyCH4V TGCA 1 cut(s) 399
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 630
HpySE526I ACGT 3 cut(s) 558, 611, 642
Hsp92II CATG 1 cut(s) 740
HspAI GCGC 1 cut(s) 361
KspI CCGCGG 1 cut(s) 584
Kzo9I GATC 2 cut(s) 600, 682
LmnI GCTCC 3 cut(s) 291, 389, 801
LweI GCATC 2 cut(s) 408, 474
MaeI CTAG 2 cut(s) 282, 408
MaeII ACGT 3 cut(s) 558, 611, 642
MaeIII GTNAC 1 cut(s) 127
MalI GATC 2 cut(s) 602, 684
MbiI CCGCTC 2 cut(s) 256, 490
MboI GATC 2 cut(s) 600, 682
MboII GAAGA 4 cut(s) 50, 218, 325, 337
MhlI GDGCHC 1 cut(s) 806
MlyI GAGTC 3 cut(s) 420, 449, 450
MmeI TCCRAC 5 cut(s) 41, 112, 244, 373, 477
MroXI GAANNNNTTC 1 cut(s) 560
MslI CAYNNNNRTG 1 cut(s) 773
MspA1I CMGCKG 1 cut(s) 583
MspI CCGG 3 cut(s) 152, 201, 621
MspR9I CCNGG 4 cut(s) 152, 225, 419, 621
MvaI CCWGG 2 cut(s) 225, 419
MvnI CGCG 3 cut(s) 309, 583, 655
MwoI GCNNNNNNNGC 2 cut(s) 119, 630
NciI CCSGG 2 cut(s) 152, 621
NdeII GATC 2 cut(s) 600, 682
NlaIII CATG 1 cut(s) 740
NlaIV GGNNCC 5 cut(s) 287, 317, 385, 803, 822
NmeAIII GCCGAG 1 cut(s) 653
NmuCI GTSAC 1 cut(s) 127
OliI CACNNNNGTG 1 cut(s) 773
PaeR7I CTCGAG 1 cut(s) 251
PcsI WCGNNNNNNNCGW 2 cut(s) 450, 537
PdmI GAANNNNTTC 1 cut(s) 560
PfeI GAWTC 2 cut(s) 167, 216
PflFI GACNNNGTC 1 cut(s) 458
PkrI GCNGC 3 cut(s) 115, 258, 657
PleI GAGTC 3 cut(s) 419, 449, 449
PmaCI CACGTG 1 cut(s) 643
PmlI CACGTG 1 cut(s) 643
PpsI GAGTC 3 cut(s) 419, 449, 449
Ppu21I YACGTR 1 cut(s) 643
Psp6I CCWGG 2 cut(s) 223, 417
PspCI CACGTG 1 cut(s) 643
PspFI CCCAGC 1 cut(s) 805
PspGI CCWGG 2 cut(s) 223, 417
PspN4I GGNNCC 5 cut(s) 287, 317, 385, 803, 822
PspPI GGNCC 5 cut(s) 300, 316, 391, 618, 820
PspXI VCTCGAGB 1 cut(s) 251
PsyI GACNNNGTC 1 cut(s) 458
RsaI GTAC 3 cut(s) 477, 578, 671
RsaNI GTAC 3 cut(s) 476, 577, 670
RseI CAYNNNNRTG 1 cut(s) 773
SacII CCGCGG 1 cut(s) 584
SatI GCNGC 3 cut(s) 114, 257, 656
Sau3AI GATC 2 cut(s) 600, 682
Sau96I GGNCC 5 cut(s) 300, 316, 391, 618, 820
ScaI AGTACT 1 cut(s) 671
SchI GAGTC 3 cut(s) 420, 449, 450
ScrFI CCNGG 4 cut(s) 152, 225, 419, 621
SduI GDGCHC 1 cut(s) 806
SfaNI GCATC 2 cut(s) 408, 474
Sfr274I CTCGAG 1 cut(s) 251
Sfr303I CCGCGG 1 cut(s) 584
SgrBI CCGCGG 1 cut(s) 584
SinI GGWCC 3 cut(s) 300, 316, 820
SlaI CTCGAG 1 cut(s) 251
SmiMI CAYNNNNRTG 1 cut(s) 773
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
SspMI CTAG 2 cut(s) 282, 408
StyD4I CCNGG 4 cut(s) 150, 223, 417, 619
StyI CCWWGG 2 cut(s) 281, 387
TaaI ACNGT 3 cut(s) 480, 755, 820
TaiI ACGT 3 cut(s) 561, 614, 645
TaqI TCGA 7 cut(s) 252, 468, 540, 546, 554, 564, 603
TaqII GACCGA 1 cut(s) 333
TatI WGTACW 1 cut(s) 669
TauI GCSGC 3 cut(s) 116, 259, 658
TfiI GAWTC 2 cut(s) 167, 216
TseFI GTSAC 1 cut(s) 127
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 3 cut(s) 17, 371, 725
TspGWI ACGGA 5 cut(s) 71, 448, 622, 677, 785
Tth111I GACNNNGTC 1 cut(s) 458
VpaK11BI GGWCC 3 cut(s) 300, 316, 820
XhoI CTCGAG 1 cut(s) 251
XmaJI CCTAGG 1 cut(s) 281
XmnI GAANNNNTTC 1 cut(s) 560
XspI CTAG 2 cut(s) 282, 408
ZrmI AGTACT 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.