Rw4G027180

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014962
Physical Location & Seq
Reverse (-)
53617261 .. 53618040
780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G027180.1

Sequence Viewer

Length: 780 bp
ATGAAAACAAGCACTCATCCACCAAAACGACGAGTCACTTTCAAATACATAACGTTGCTTTCTTCCTTCCTCTTCCTCCCTGTCCTCTATTTCTTCCTTCACGTCAAGCACAACCTCCACCCCACCGCCATCGTTGACCACTCACTGCCGCACCACGTCCAAGTCCCCGACATCCGTATCCGCTCCGGCTACACCTCCTACAAGACCTACCTCCAGCACCAGCTCAACAAGACCCTCAACCCTAAACTCCGCAAAGTCTGGACCACCCGCGACTGGGACCGCAAAATCCAGGTCTTCTCCGAGATTTTCACTCGGTTGAAGCAGGAAAAGCTCCTGTCAAATAACTCTCGGGCGCTGTGTATTGGCGCTCGAGTAGGGCAGGAGGTCCAGGCTCTGAAACGAATAGGGGTGAATGACTCGATCGGGATGGACCTCGTGCCCTACCCTCCGCTGGTTGTGGAGGGGGACTTTCACAGCCAGCCTTTTCAGAACGAGACGTTCGACTTCGAGTTCTCGAACGTGTTCGATCATGCGCTGTACCCTAGGAAGTTCGTGGCGGAGATCGAACGGACGTTGAGGCGGCGCGGGGTGTGTGTGTTACACGTGGCGATATCGAAGCGGGGGGATAAGTACTCGGCCAACGACCTGTTCAGTGTGCGGCCATTGGTGGAGATGTTTCAGAGGTCCGAGTTGGTTTATGTCAGGAAGGTTGACGGGTTTGGGTTGGACACCGAGGTCGTCTTTCGGAAACGGGAAGTCAAACTCATCCAACAACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

259

Amino Acids

30.43

Weight (kDa)

9.97

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 119 - 200 3.7e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014962)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24480 AT4G24805
fragaria_vesca FvH4_4g25070
malus_domestica MD13G1106800.v1.1 MD16G1106900.v1.1
prunus_persica Prupe.1G239500_v2.0.a1
pyrus_communis pycom13g09260
rosa_chinensis RchiOBHm_Chr4g0432251
rosa_laevigata RLG00000006859
rosa_multiflora Rmu_co8384597.1_g000001
rosa_roxburghii Rroxscaffold_5G00373700
rosa_rugosa Rorug04G0255600
rosa_samantha Rh4BG320200 Rh4CG335400 Rh4DG315600
rosa_wichuraiana Rw4G027180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 734
AccBSI CCGCTC 1 cut(s) 183
AccII CGCG 2 cut(s) 270, 585
AclI AACGTT 1 cut(s) 53
AcoI YGGCCR 2 cut(s) 636, 659
AcvI CACGTG 1 cut(s) 604
AfaI GTAC 2 cut(s) 539, 632
AfiI CCNNNNNNNGG 3 cut(s) 273, 274, 451
AflIII ACRYGT 2 cut(s) 519, 601
AgsI TTSAA 2 cut(s) 43, 319
AjiI CACGTC 2 cut(s) 103, 157
AjnI CCWGG 2 cut(s) 288, 387
AjuI GAANNNNNNNTTGG 2 cut(s) 632, 664
AluBI AGCT 2 cut(s) 223, 331
AluI AGCT 2 cut(s) 223, 331
Alw26I GTCTC 1 cut(s) 488
AlwNI CAGNNNCTG 1 cut(s) 394
Ama87I CYCGRG 2 cut(s) 348, 369
AoxI GGCC 2 cut(s) 636, 659
ArsI GACNNNNNNTTYG 2 cut(s) 276, 308
Asp700I GAANNNNTTC 1 cut(s) 521
AspA2I CCTAGG 1 cut(s) 542
AspLEI GCGC 4 cut(s) 355, 368, 535, 585
AspS9I GGNCC 5 cut(s) 261, 277, 385, 430, 684
AsuHPI GGTGA 1 cut(s) 421
AvaI CYCGRG 2 cut(s) 348, 369
AvaII GGWCC 5 cut(s) 261, 277, 385, 430, 684
AvrII CCTAGG 1 cut(s) 542
BaeGI GKGCMC 1 cut(s) 441
BaeI ACNNNNGTAYC 2 cut(s) 160, 193
BauI CACGAG 1 cut(s) 434
BbrPI CACGTG 1 cut(s) 604
BbsI GAAGAC 1 cut(s) 286
BccI CCATC 2 cut(s) 137, 421
BciT130I CCWGG 2 cut(s) 290, 389
BciVI GTATCC 1 cut(s) 188
BcoDI GTCTC 1 cut(s) 488
BfaI CTAG 1 cut(s) 543
BfoI RGCGCY 2 cut(s) 356, 369
BfuI GTATCC 1 cut(s) 188
BisI GCNGC 3 cut(s) 149, 581, 659
BlnI CCTAGG 1 cut(s) 542
BlsI GCNGC 3 cut(s) 150, 582, 660
BmcAI AGTACT 1 cut(s) 632
Bme1390I CCNGG 2 cut(s) 290, 389
Bme18I GGWCC 5 cut(s) 261, 277, 385, 430, 684
BmeT110I CYCGRG 2 cut(s) 348, 369
BmgBI CACGTC 2 cut(s) 103, 157
BmgT120I GGNCC 5 cut(s) 261, 277, 385, 430, 684
BmiI GGNNCC 1 cut(s) 278
BmrFI CCNGG 2 cut(s) 290, 389
BmrI ACTGGG 1 cut(s) 283
BmuI ACTGGG 1 cut(s) 283
BpiI GAAGAC 1 cut(s) 286
BpmI CTGGAG 1 cut(s) 197
BsaAI YACGTR 1 cut(s) 604
BsaJI CCNNGG 2 cut(s) 542, 732
Bsc4I CCNNNNNNNGG 3 cut(s) 273, 274, 451
Bse1I ACTGG 1 cut(s) 278
BseBI CCWGG 2 cut(s) 290, 389
BseDI CCNNGG 2 cut(s) 542, 732
BseGI GGATG 4 cut(s) 16, 171, 432, 765
BseLI CCNNNNNNNGG 3 cut(s) 273, 274, 451
BseNI ACTGG 1 cut(s) 278
BseSI GKGCMC 1 cut(s) 441
Bsh1236I CGCG 2 cut(s) 270, 585
Bsh1285I CGRYCG 1 cut(s) 423
BshFI GGCC 2 cut(s) 638, 661
BsiEI CGRYCG 1 cut(s) 423
BsiHKCI CYCGRG 2 cut(s) 348, 369
BsiSI CCGG 1 cut(s) 186
BslFI GGGAC 3 cut(s) 149, 290, 479
BslI CCNNNNNNNGG 3 cut(s) 273, 274, 451
BsmAI GTCTC 1 cut(s) 488
BsmBI CGTCTC 1 cut(s) 488
BsmFI GGGAC 3 cut(s) 149, 290, 479
BsnI GGCC 2 cut(s) 638, 661
BsoBI CYCGRG 2 cut(s) 348, 369
Bsp1286I GDGCHC 1 cut(s) 441
Bsp143I GATC 3 cut(s) 420, 526, 561
BspANI GGCC 2 cut(s) 638, 661
BspFNI CGCG 2 cut(s) 270, 585
BspLI GGNNCC 1 cut(s) 278
BsrBI CCGCTC 1 cut(s) 183
BsrI ACTGG 1 cut(s) 278
BssECI CCNNGG 2 cut(s) 542, 732
BssMI GATC 3 cut(s) 420, 526, 561
BssSI CACGAG 1 cut(s) 434
BssT1I CCWWGG 1 cut(s) 542
Bst2BI CACGAG 1 cut(s) 434
Bst2UI CCWGG 2 cut(s) 290, 389
Bst4CI ACNGT 1 cut(s) 777
Bst6I CTCTTC 1 cut(s) 77
BstBAI YACGTR 1 cut(s) 604
BstC8I GCNNGC 1 cut(s) 479
BstF5I GGATG 4 cut(s) 16, 171, 432, 765
BstFNI CGCG 2 cut(s) 270, 585
BstH2I RGCGCY 2 cut(s) 356, 369
BstHHI GCGC 4 cut(s) 355, 368, 535, 585
BstKTI GATC 3 cut(s) 423, 529, 564
BstMAI GTCTC 1 cut(s) 488
BstMBI GATC 3 cut(s) 420, 526, 561
BstMCI CGRYCG 1 cut(s) 423
BstMWI GCNNNNNNNGC 1 cut(s) 328
BstNI CCWGG 2 cut(s) 290, 389
BstSCI CCNGG 2 cut(s) 288, 387
BstSLI GKGCMC 1 cut(s) 441
BstUI CGCG 2 cut(s) 270, 585
BstV2I GAAGAC 1 cut(s) 286
BsuI GTATCC 1 cut(s) 188
BsuRI GGCC 2 cut(s) 638, 661
BtrI CACGTC 2 cut(s) 103, 157
BtsCI GGATG 4 cut(s) 16, 171, 432, 765
BtsI GCAGTG 1 cut(s) 143
BtsIMutI CAGTG 2 cut(s) 143, 658
Cac8I GCNNGC 1 cut(s) 479
CaiI CAGNNNCTG 1 cut(s) 394
CfoI GCGC 4 cut(s) 355, 368, 535, 585
Cfr13I GGNCC 5 cut(s) 261, 277, 385, 430, 684
Csp6I GTAC 2 cut(s) 538, 631
CviAII CATG 1 cut(s) 530
CviJI RGCY 8 cut(s) 189, 223, 331, 392, 477, 481, 638, 661
CviKI_1 RGCY 8 cut(s) 189, 223, 331, 392, 477, 481, 638, 661
CviQI GTAC 2 cut(s) 538, 631
DpnI GATC 3 cut(s) 422, 528, 563
DpnII GATC 3 cut(s) 420, 526, 561
DrdI GACNNNNNNGTC 1 cut(s) 734
DseDI GACNNNNNNGTC 1 cut(s) 734
EaeI YGGCCR 2 cut(s) 636, 659
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
EciI GGCGGA 1 cut(s) 572
Eco130I CCWWGG 1 cut(s) 542
Eco32I GATATC 1 cut(s) 612
Eco47I GGWCC 5 cut(s) 261, 277, 385, 430, 684
Eco72I CACGTG 1 cut(s) 604
Eco88I CYCGRG 2 cut(s) 348, 369
EcoRII CCWGG 2 cut(s) 288, 387
EcoRV GATATC 1 cut(s) 612
EcoT14I CCWWGG 1 cut(s) 542
ErhI CCWWGG 1 cut(s) 542
Esp3I CGTCTC 1 cut(s) 488
FaeI CATG 1 cut(s) 533
FaiI YATR 3 cut(s) 50, 531, 699
FaqI GGGAC 3 cut(s) 149, 290, 479
FatI CATG 1 cut(s) 529
FauI CCCGC 3 cut(s) 275, 578, 612
Fnu4HI GCNGC 3 cut(s) 149, 581, 659
FokI GGATG 4 cut(s) 3, 158, 439, 752
Fsp4HI GCNGC 3 cut(s) 149, 581, 659
FspBI CTAG 1 cut(s) 543
GlaI GCGC 4 cut(s) 354, 367, 534, 584
GluI GCNGC 3 cut(s) 149, 581, 659
GsuI CTGGAG 1 cut(s) 197
HaeII RGCGCY 2 cut(s) 356, 369
HaeIII GGCC 2 cut(s) 638, 661
HapII CCGG 1 cut(s) 186
HhaI GCGC 4 cut(s) 355, 368, 535, 585
Hin1II CATG 1 cut(s) 533
Hin6I GCGC 4 cut(s) 353, 366, 533, 583
HinP1I GCGC 4 cut(s) 353, 366, 533, 583
HincII GTYRAC 2 cut(s) 136, 712
HindII GTYRAC 2 cut(s) 136, 712
HinfI GANTC 2 cut(s) 33, 416
HpaII CCGG 1 cut(s) 186
HphI GGTGA 1 cut(s) 421
Hpy166II GTNNAC 2 cut(s) 136, 712
Hpy188I TCNGA 6 cut(s) 301, 396, 489, 681, 688, 747
Hpy188III TCNNGA 4 cut(s) 259, 424, 514, 703
Hpy8I GTNNAC 2 cut(s) 136, 712
Hpy99I CGWCG 1 cut(s) 33
HpyAV CCTTC 3 cut(s) 76, 107, 700
HpyCH4III ACNGT 1 cut(s) 777
HpyCH4IV ACGT 7 cut(s) 53, 102, 156, 497, 519, 572, 603
HpyF10VI GCNNNNNNNGC 1 cut(s) 328
HpySE526I ACGT 7 cut(s) 53, 102, 156, 497, 519, 572, 603
Hsp92II CATG 1 cut(s) 533
HspAI GCGC 4 cut(s) 353, 366, 533, 583
Kzo9I GATC 3 cut(s) 420, 526, 561
LmnI GCTCC 2 cut(s) 188, 336
MaeI CTAG 1 cut(s) 543
MaeII ACGT 7 cut(s) 53, 102, 156, 497, 519, 572, 603
MaeIII GTNAC 2 cut(s) 34, 597
MalI GATC 3 cut(s) 422, 528, 563
MbiI CCGCTC 1 cut(s) 183
MboI GATC 3 cut(s) 420, 526, 561
MboII GAAGA 4 cut(s) 54, 64, 85, 286
MhlI GDGCHC 1 cut(s) 441
MlyI GAGTC 2 cut(s) 42, 410
MmeI TCCRAC 1 cut(s) 705
MroXI GAANNNNTTC 1 cut(s) 521
MspA1I CMGCKG 1 cut(s) 451
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 2 cut(s) 290, 389
MvaI CCWGG 2 cut(s) 290, 389
MvnI CGCG 2 cut(s) 270, 585
MwoI GCNNNNNNNGC 1 cut(s) 328
NdeII GATC 3 cut(s) 420, 526, 561
NlaIII CATG 1 cut(s) 533
NlaIV GGNNCC 1 cut(s) 278
NmeAIII GCCGAG 1 cut(s) 614
NmuCI GTSAC 1 cut(s) 34
PaeR7I CTCGAG 1 cut(s) 369
PcsI WCGNNNNNNNCGW 1 cut(s) 498
PdmI GAANNNNTTC 1 cut(s) 521
PkrI GCNGC 3 cut(s) 150, 582, 660
Ple19I CGATCG 1 cut(s) 423
PleI GAGTC 2 cut(s) 41, 410
PmaCI CACGTG 1 cut(s) 604
PmlI CACGTG 1 cut(s) 604
PpsI GAGTC 2 cut(s) 41, 410
Ppu21I YACGTR 1 cut(s) 604
Psp1406I AACGTT 1 cut(s) 53
Psp6I CCWGG 2 cut(s) 288, 387
PspCI CACGTG 1 cut(s) 604
PspGI CCWGG 2 cut(s) 288, 387
PspN4I GGNNCC 1 cut(s) 278
PspPI GGNCC 5 cut(s) 261, 277, 385, 430, 684
PspXI VCTCGAGB 1 cut(s) 369
PstNI CAGNNNCTG 1 cut(s) 394
PvuI CGATCG 1 cut(s) 423
RsaI GTAC 2 cut(s) 539, 632
RsaNI GTAC 2 cut(s) 538, 631
SatI GCNGC 3 cut(s) 149, 581, 659
Sau3AI GATC 3 cut(s) 420, 526, 561
Sau96I GGNCC 5 cut(s) 261, 277, 385, 430, 684
ScaI AGTACT 1 cut(s) 632
SchI GAGTC 2 cut(s) 42, 410
ScrFI CCNGG 2 cut(s) 290, 389
SduI GDGCHC 1 cut(s) 441
Sfr274I CTCGAG 1 cut(s) 369
SinI GGWCC 5 cut(s) 261, 277, 385, 430, 684
SlaI CTCGAG 1 cut(s) 369
SmlI CTYRAG 1 cut(s) 369
SmoI CTYRAG 1 cut(s) 369
SspMI CTAG 1 cut(s) 543
StyD4I CCNGG 2 cut(s) 288, 387
StyI CCWWGG 1 cut(s) 542
TaaI ACNGT 1 cut(s) 777
TaiI ACGT 7 cut(s) 56, 105, 159, 500, 522, 575, 606
TaqI TCGA 8 cut(s) 370, 419, 501, 507, 515, 525, 564, 614
TatI WGTACW 1 cut(s) 630
TauI GCSGC 3 cut(s) 151, 583, 661
TscAI CASTG 2 cut(s) 150, 658
TseFI GTSAC 1 cut(s) 34
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 164, 583
TspRI CASTG 2 cut(s) 150, 658
VpaK11BI GGWCC 5 cut(s) 261, 277, 385, 430, 684
XhoI CTCGAG 1 cut(s) 369
XmaJI CCTAGG 1 cut(s) 542
XmnI GAANNNNTTC 1 cut(s) 521
XspI CTAG 1 cut(s) 543
ZrmI AGTACT 1 cut(s) 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.