Rh4DG315600

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014962
Physical Location & Seq
Reverse (-)
53874167 .. 53874520
354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG315600.1

Sequence Viewer

Length: 354 bp
ATGGACCTAGTGCCCTACCCTCCGCTGGTTGTGGAGGGGGACTTTCACAGCCAGCCTTTTCAGAACGAGACGTTCGACTTCGAATTCTCGAACGTGTTCGATCACGCCCTGTACCCTAGGAAGTTCGTGGCGGAGATCGAGCGGACGTTGAGGCGGCGCGGGGTGTGTGTGTTACACGTGGCCATATCGAAGCGGGGGGATAAGTACTCGGCCAACGACCTGTTCAGTGTGCGGCCATTGGTGGAGATGTTTCAGAGGTCCGAGTTGGTTTATGTCAGGAAGGTTGACGGGTTTGGGTTGGACACCGAGGTCGTCTTTCGGAAACGGGAAGTCAAACTCATCCAACAACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

117

Amino Acids

13.81

Weight (kDa)

7.9

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 10 - 58 1.4e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014962)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24480 AT4G24805
fragaria_vesca FvH4_4g25070
malus_domestica MD13G1106800.v1.1 MD16G1106900.v1.1
prunus_persica Prupe.1G239500_v2.0.a1
pyrus_communis pycom13g09260
rosa_chinensis RchiOBHm_Chr4g0432251
rosa_laevigata RLG00000006859
rosa_multiflora Rmu_co8384597.1_g000001
rosa_roxburghii Rroxscaffold_5G00373700
rosa_rugosa Rorug04G0255600
rosa_samantha Rh4BG320200 Rh4CG335400 Rh4DG315600
rosa_wichuraiana Rw4G027180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 308
AccBSI CCGCTC 1 cut(s) 142
AccII CGCG 1 cut(s) 159
AciI CCGC 7 cut(s) 23, 131, 142, 154, 159, 193, 232
AcoI YGGCCR 3 cut(s) 180, 210, 233
AcsI RAATTY 1 cut(s) 83
AcvI CACGTG 1 cut(s) 178
AfaI GTAC 2 cut(s) 113, 206
AfiI CCNNNNNNNGG 1 cut(s) 25
AflIII ACRYGT 2 cut(s) 93, 175
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
Alw26I GTCTC 1 cut(s) 62
AoxI GGCC 3 cut(s) 180, 210, 233
ApoI RAATTY 1 cut(s) 83
Asp700I GAANNNNTTC 1 cut(s) 95
AspA2I CCTAGG 1 cut(s) 116
AspLEI GCGC 1 cut(s) 159
AspS9I GGNCC 2 cut(s) 4, 258
AsuII TTCGAA 1 cut(s) 81
AvaII GGWCC 2 cut(s) 4, 258
AvrII CCTAGG 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 15
BalI TGGCCA 1 cut(s) 182
BbrPI CACGTG 1 cut(s) 178
BcoDI GTCTC 1 cut(s) 62
BfaI CTAG 2 cut(s) 8, 117
BisI GCNGC 2 cut(s) 155, 233
BlnI CCTAGG 1 cut(s) 116
BlsI GCNGC 2 cut(s) 156, 234
BmcAI AGTACT 1 cut(s) 206
Bme18I GGWCC 2 cut(s) 4, 258
BmgT120I GGNCC 2 cut(s) 4, 258
Bpu14I TTCGAA 1 cut(s) 81
BsaAI YACGTR 1 cut(s) 178
BsaJI CCNNGG 2 cut(s) 116, 306
Bsc4I CCNNNNNNNGG 1 cut(s) 25
BseDI CCNNGG 2 cut(s) 116, 306
BseGI GGATG 1 cut(s) 339
BseLI CCNNNNNNNGG 1 cut(s) 25
BseSI GKGCMC 1 cut(s) 15
Bsh1236I CGCG 1 cut(s) 159
BshFI GGCC 3 cut(s) 182, 212, 235
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 1 cut(s) 25
BsmAI GTCTC 1 cut(s) 62
BsmBI CGTCTC 1 cut(s) 62
BsmFI GGGAC 1 cut(s) 53
BsnI GGCC 3 cut(s) 182, 212, 235
Bsp119I TTCGAA 1 cut(s) 81
Bsp1286I GDGCHC 1 cut(s) 15
Bsp143I GATC 2 cut(s) 100, 135
BspACI CCGC 7 cut(s) 23, 131, 142, 154, 159, 193, 232
BspANI GGCC 3 cut(s) 182, 212, 235
BspFNI CGCG 1 cut(s) 159
BspT104I TTCGAA 1 cut(s) 81
BsrBI CCGCTC 1 cut(s) 142
BssECI CCNNGG 2 cut(s) 116, 306
BssMI GATC 2 cut(s) 100, 135
BssT1I CCWWGG 1 cut(s) 116
Bst4CI ACNGT 1 cut(s) 351
BstBAI YACGTR 1 cut(s) 178
BstBI TTCGAA 1 cut(s) 81
BstC8I GCNNGC 1 cut(s) 53
BstF5I GGATG 1 cut(s) 339
BstFNI CGCG 1 cut(s) 159
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 2 cut(s) 103, 138
BstMAI GTCTC 1 cut(s) 62
BstMBI GATC 2 cut(s) 100, 135
BstSLI GKGCMC 1 cut(s) 15
BstUI CGCG 1 cut(s) 159
BsuRI GGCC 3 cut(s) 182, 212, 235
BtsCI GGATG 1 cut(s) 339
BtsIMutI CAGTG 1 cut(s) 232
Cac8I GCNNGC 1 cut(s) 53
CfoI GCGC 1 cut(s) 159
Cfr13I GGNCC 2 cut(s) 4, 258
Csp6I GTAC 2 cut(s) 112, 205
CviJI RGCY 5 cut(s) 51, 55, 182, 212, 235
CviKI_1 RGCY 5 cut(s) 51, 55, 182, 212, 235
CviQI GTAC 2 cut(s) 112, 205
DpnI GATC 2 cut(s) 102, 137
DpnII GATC 2 cut(s) 100, 135
DrdI GACNNNNNNGTC 1 cut(s) 308
DseDI GACNNNNNNGTC 1 cut(s) 308
EaeI YGGCCR 3 cut(s) 180, 210, 233
EciI GGCGGA 1 cut(s) 146
Eco130I CCWWGG 1 cut(s) 116
Eco47I GGWCC 2 cut(s) 4, 258
Eco72I CACGTG 1 cut(s) 178
EcoRI GAATTC 1 cut(s) 83
EcoT14I CCWWGG 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 116
Esp3I CGTCTC 1 cut(s) 62
FaiI YATR 2 cut(s) 185, 273
FaqI GGGAC 1 cut(s) 53
FauI CCCGC 2 cut(s) 152, 186
Fnu4HI GCNGC 2 cut(s) 155, 233
FokI GGATG 1 cut(s) 326
Fsp4HI GCNGC 2 cut(s) 155, 233
FspBI CTAG 2 cut(s) 8, 117
GlaI GCGC 1 cut(s) 158
GluI GCNGC 2 cut(s) 155, 233
HaeIII GGCC 3 cut(s) 182, 212, 235
HhaI GCGC 1 cut(s) 159
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
Hpy166II GTNNAC 1 cut(s) 286
Hpy188I TCNGA 4 cut(s) 63, 255, 262, 321
Hpy188III TCNNGA 2 cut(s) 88, 277
Hpy8I GTNNAC 1 cut(s) 286
HpyAV CCTTC 1 cut(s) 274
HpyCH4III ACNGT 1 cut(s) 351
HpyCH4IV ACGT 4 cut(s) 71, 93, 146, 177
HpySE526I ACGT 4 cut(s) 71, 93, 146, 177
HspAI GCGC 1 cut(s) 157
Kzo9I GATC 2 cut(s) 100, 135
LpnPI CCDG 5 cut(s) 11, 65, 122, 233, 262
MaeI CTAG 2 cut(s) 8, 117
MaeII ACGT 4 cut(s) 71, 93, 146, 177
MaeIII GTNAC 1 cut(s) 171
MalI GATC 2 cut(s) 102, 137
MbiI CCGCTC 1 cut(s) 142
MboI GATC 2 cut(s) 100, 135
MhlI GDGCHC 1 cut(s) 15
MlsI TGGCCA 1 cut(s) 182
MluCI AATT 1 cut(s) 83
MluNI TGGCCA 1 cut(s) 182
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 5 cut(s) 28, 30, 144, 249, 301
Mox20I TGGCCA 1 cut(s) 182
MroXI GAANNNNTTC 1 cut(s) 95
MscI TGGCCA 1 cut(s) 182
Msp20I TGGCCA 1 cut(s) 182
MspA1I CMGCKG 1 cut(s) 25
MvnI CGCG 1 cut(s) 159
NdeII GATC 2 cut(s) 100, 135
NmeAIII GCCGAG 1 cut(s) 188
NspV TTCGAA 1 cut(s) 81
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PdmI GAANNNNTTC 1 cut(s) 95
PkrI GCNGC 2 cut(s) 156, 234
PmaCI CACGTG 1 cut(s) 178
PmlI CACGTG 1 cut(s) 178
Ppu21I YACGTR 1 cut(s) 178
PspCI CACGTG 1 cut(s) 178
PspPI GGNCC 2 cut(s) 4, 258
RsaI GTAC 2 cut(s) 113, 206
RsaNI GTAC 2 cut(s) 112, 205
SatI GCNGC 2 cut(s) 155, 233
Sau3AI GATC 2 cut(s) 100, 135
Sau96I GGNCC 2 cut(s) 4, 258
ScaI AGTACT 1 cut(s) 206
SduI GDGCHC 1 cut(s) 15
SetI ASST 9 cut(s) 9, 74, 96, 149, 180, 222, 260, 285, 312
SfuI TTCGAA 1 cut(s) 81
SinI GGWCC 2 cut(s) 4, 258
Sse9I AATT 1 cut(s) 83
SsiI CCGC 7 cut(s) 23, 131, 142, 154, 159, 193, 232
SspMI CTAG 2 cut(s) 8, 117
StyI CCWWGG 1 cut(s) 116
TaaI ACNGT 1 cut(s) 351
TaiI ACGT 4 cut(s) 74, 96, 149, 180
TaqI TCGA 6 cut(s) 75, 81, 89, 99, 138, 188
TasI AATT 1 cut(s) 83
TatI WGTACW 1 cut(s) 204
TauI GCSGC 2 cut(s) 157, 235
TscAI CASTG 1 cut(s) 232
TspRI CASTG 1 cut(s) 232
VpaK11BI GGWCC 2 cut(s) 4, 258
XapI RAATTY 1 cut(s) 83
XmaJI CCTAGG 1 cut(s) 116
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 2 cut(s) 8, 117
ZrmI AGTACT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.