Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004792

Physical Location & Seq

Genomic Coordinates Reverse (-)

10046385 .. 10050842

Gene Structure 4458 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1131600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2064 bp

ATGCTGGCCAAAGTTCCTGCTCCGGCGGCAGTCAACCACCACCACCACCACCTCCTGCGGCCAAAACGCCTTGAACCAGAATCATGGATGGCGGGAACCCACAGCTGCAAGTGTCTCTATCTTCATCAGACGAGAAAATGGGGGATTAGTTGCAGTTTCAGTCAGAGACCCACCGATAACCCTGCAGCTGCAGCAGAAGCTTCTTATGACCTCATGGACGATTTCATCGTCCTTGGACAAGACAACACTGCCCCTCCCACTCCCACCACCAGAAACACTACCACCCATCGAGGCAATGACCGTCAACCGGCACCTACTAGTAGGAGAGTTGCTCAAAGTTTCGGGAGATTGAAAGTACATAGAGTGAAATCTATAGCTGAGAAACCTTCTCGGATTAAGCAAGAAATAGATGAAAGTGATGATGAAGTAAATGTGCAAGATGCACCACCATTTGTCAATTCGCCTTCAAGACTTGAATTTGATGGGAAAAAGAACAGAGCAGATGCCCGCGGTCTTGGTGGAAGACGCTCAAGTGATTCCCACTCGAAAGAATTTAGGGACATGAGGCGGAGGAAGGGGACAGCAAACAACATGGACCATTCTCCTGTGAAAAGGACGGAAAATGGTTTTGAGCCAATCTCTGACAATCAAAACAAACAACATAAGCCTTATAGGTCTGACACCAACATAGCCAGACAAAAGGATTCAGCTCCAAGAGGTTCTTCTGCTCACTCAAAAGGATGGGGCAATGGAGGAGGGTCCATGTATGACTTGGCTGAGTTGCCCGACCTAAATCAGCGAAGGAAATTTTCCACTGACAATGACTTTTTCAGTCGAAAATCCTTTAGGGATGTGGGTTGCAGTGAATATATGATTGAATGTCTGAAGAGGCAGCTTTTCCAGCGCCCATCGCATATTCAGGCCATGGCATTTGCTCCTGTTGTTGAGGGGAAAACGTCTATTATAGCAGACCAAAGTGGATCTGGCAAGACATTAGCTTATCTTGCTCCAGTAATTCAGCGTTTAAGGGAAGAAGAACTTCGAGGGCTGAGCAAATCCTCATCACTGAGTCCTCGAGTTGTTATACTGGTACCAACTGCTGAGTTAGCTTCTCAGGTTTTGGGTGTTTGCCGTTCAATGTCAAAATTTGGGGTTCCAGTTAGGTCTATGGTTGTAACTGGTGGTCATCGGCAGAAAACTCAGATGGAAAGTTTAGAACAAGGTGTGGATATCCTAATAGCAACACCTGGTCGGTTTATGTATCTGATTAAAGAGGGCTTCGTGCACCTATCAAATCTAAGATGCGCTGTACTAGATGAGGTAGATATACTCTTTAATGATGAGGATTTTGAAGCAGCACTGGAAAGTTTGATCAATTCTGCACCTGTTACCACACAATATCTTTTTGTGACTGCAACGTTACCCCTTGGAATTTACAACAAATTGGTTGAATCCTTTCCTGATTGTAAAGTAGTCATGGCACCTGGAATGCACCGTATAAGCTCTGGCCTTGAAGAGGTGCTTGTAGATTGCAGTGGAGATGATGGAGGTGAAAGATCTCCAGAGAAGGCATTCTCAAATAAGAAATCTGCTCTTCTACAGCTTGTGGAGGGAAGTCCAGTTCCCAAATCGATAATTTTCTGCAACAAGATTGAAACGTGCAGAAAAGTTGAGAATATATTAACACGTTTTGACAGAAGTGGAACTCGCGTTCGGGTTCTACCATTCCACTCTGCCTTGGCCCAAGGATCAAGACTCGCAAATATGGAGGAGTTCACCAATTCTCATTCAAAGGAAGTCTCACAGTTTTTGGTTTGCACTGATAGAGCATCGCGTGGAATCGACTTTTCTGGCGTGGATCATGTTATCCTCTTTGACTTCCCCCGCGATCCAAGTGAGTATGTACGACGTGTTGGAAGAACTGCCCGAGGTGCTGGAGGAATCGGAAAGGCGTTCATCTTTGTGGTGGGGAAACAAGTCTCCCTTGCGGGAAAGATAATGGAAAGAAACCGGAAAGGTCACCCGGTGCATGATGTACCAGCTGCATATGAGCTTTTGTATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002376 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006968 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009753 GO:0009814 GO:0009817 GO:0009987 GO:0010033 GO:0010501 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0032870 GO:0034641 GO:0035690 GO:0042221 GO:0042493 GO:0042623 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046483 GO:0046677 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0070013 GO:0070035 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0090304 GO:0098542 GO:0140098 GO:1901360 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

688

Amino Acids

76.33

Weight (kDa)

9.23

Isoelectric Point (pI)

55.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	305 - 475	1e-33	DEAD/DEAH box helicase
Helicase_C	PF00271	528 - 643	7.1e-23	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004792)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06980
fragaria_vesca	FvH4_4g21860 FvH4_4g21860 FvH4_4g21860 FvH4_4g21860 FvH4_4g21860 FvH4_4g21860 FvH4_4g21860 FvH4_4g21860 FvH4_4g21860
malus_domestica	MD16G1131600.v1.1
prunus_persica	Prupe.1G209200_v2.0.a1
pyrus_communis	pycom16g11600
rosa_chinensis	RchiOBHm_Chr4g0410871 RchiOBHm_Chr4g0428121
rosa_laevigata	RLG00000007180 RLG00000008393
rosa_multiflora	Rmu_sc0004940.1_g000014 Rmu_sc0007108.1_g000006 Rmu_sc0009599.1_g000014
rosa_roxburghii	Rroxscaffold_5G00369830
rosa_rugosa	Rorug04G0222400
rosa_samantha	Rh4AG163400 Rh4AG279700 Rh4BG285100 Rh4CG176300 Rh4CG300700 Rh4DG159100 Rh4DG282200
rosa_wichuraiana	Rw0G005430 Rw4G024300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1090
AccB1I	GGYRCC	3 cut(s)	310, 1090, 1480
AccII	CGCG	4 cut(s)	510, 1710, 1834, 1887
AciI	CCGC	8 cut(s)	26, 58, 92, 508, 510, 568, 1885, 1988
AclI	AACGTT	1 cut(s)	1418
AclWI	GGATC	4 cut(s)	988, 1756, 1866, 1883
AcoI	YGGCCR	2 cut(s)	6, 59
AcsI	RAATTY	5 cut(s)	476, 551, 806, 1145, 1431
AcuI	CTGAAG	1 cut(s)	905
AdeI	CACNNNGTG	1 cut(s)	2026
AfaI	GTAC	5 cut(s)	357, 1092, 1311, 1905, 2037
AfiI	CCNNNNNNNGG	2 cut(s)	307, 1516
AflIII	ACRYGT	2 cut(s)	1685, 1909
AhlI	ACTAGT	1 cut(s)	317
AjiI	CACGTC	1 cut(s)	1910
AjnI	CCWGG	2 cut(s)	1246, 1483
AjuI	GAANNNNNNNTTGG	2 cut(s)	706, 738
AloI	GAACNNNNNNTCC	4 cut(s)	1605, 1637, 1695, 1727
Alw21I	GWGCWC	1 cut(s)	1287
Alw26I	GTCTC	4 cut(s)	119, 160, 1804, 1984
Alw44I	GTGCAC	1 cut(s)	1283
AlwI	GGATC	4 cut(s)	988, 1756, 1866, 1883
Ama87I	CYCGRG	2 cut(s)	1074, 1926
AoxI	GGCC	5 cut(s)	6, 59, 921, 1507, 1740
ApaLI	GTGCAC	1 cut(s)	1283
ApeKI	GCWGC	7 cut(s)	105, 185, 188, 191, 892, 1355, 2042
ApoI	RAATTY	5 cut(s)	476, 551, 806, 1145, 1431
Asp700I	GAANNNNTTC	3 cut(s)	1038, 1455, 1571
Asp718I	GGTACC	1 cut(s)	1090
AspLEI	GCGC	2 cut(s)	906, 1307
AspS9I	GGNCC	3 cut(s)	595, 759, 1741
AsuC2I	CCSGG	1 cut(s)	2024
AsuHPI	GGTGA	3 cut(s)	1562, 1768, 2012
AvaI	CYCGRG	2 cut(s)	1074, 1926
AvaII	GGWCC	2 cut(s)	595, 759
BaeGI	GKGCMC	1 cut(s)	1287
BalI	TGGCCA	1 cut(s)	8
BanI	GGYRCC	3 cut(s)	310, 1090, 1480
BbsI	GAAGAC	1 cut(s)	529
Bbv12I	GWGCWC	1 cut(s)	1287
BbvI	GCAGC	7 cut(s)	92, 175, 197, 203, 904, 1367, 2029
BccI	CCATC	7 cut(s)	82, 294, 476, 735, 916, 1198, 1538
BceAI	ACGGC	1 cut(s)	1116
BcgI	CGANNNNNNTGC	2 cut(s)	164, 198
BciT130I	CCWGG	2 cut(s)	1248, 1485
BclI	TGATCA	1 cut(s)	1371
BcnI	CCSGG	1 cut(s)	2024
BcoDI	GTCTC	4 cut(s)	119, 160, 1804, 1984
BcuI	ACTAGT	1 cut(s)	317
BfaI	CTAG	2 cut(s)	318, 1313
BfmI	CTRYAG	4 cut(s)	183, 189, 372, 1598
BfoI	RGCGCY	1 cut(s)	907
BglII	AGATCT	1 cut(s)	1556
BisI	GCNGC	9 cut(s)	27, 59, 106, 186, 189, 192, 893, 1356, 2043
BlpI	GCTNAGC	1 cut(s)	1049
BlsI	GCNGC	9 cut(s)	28, 60, 107, 187, 190, 193, 894, 1357, 2044
Bme1390I	CCNGG	3 cut(s)	1248, 1485, 2024
Bme18I	GGWCC	2 cut(s)	595, 759
BmeT110I	CYCGRG	2 cut(s)	1074, 1926
BmgBI	CACGTC	1 cut(s)	1910
BmgT120I	GGNCC	3 cut(s)	595, 759, 1741
BmiI	GGNNCC	6 cut(s)	97, 312, 760, 1092, 1155, 1482
BmrFI	CCNGG	3 cut(s)	1248, 1485, 2024
BmsI	GCATC	4 cut(s)	430, 493, 1292, 1838
BpiI	GAAGAC	1 cut(s)	529
BplI	GAGNNNNNCTC	4 cut(s)	316, 348, 623, 655
BpmI	CTGGAG	3 cut(s)	993, 1545, 1956
Bpu1102I	GCTNAGC	1 cut(s)	1049
BpuEI	CTTGAG	1 cut(s)	514
BpuMI	CCSGG	1 cut(s)	2024
Bsa29I	ATCGAT	1 cut(s)	1631
BsaI	GGTCTC	1 cut(s)	160
BsaJI	CCNNGG	7 cut(s)	232, 508, 924, 1426, 1737, 1744, 1927
BsaWI	WCCGGW	1 cut(s)	2010
BsaXI	ACNNNNNCTCC	2 cut(s)	238, 268
Bsc4I	CCNNNNNNNGG	2 cut(s)	307, 1516
Bse118I	RCCGGY	1 cut(s)	307
Bse1I	ACTGG	6 cut(s)	1010, 1092, 1157, 1183, 1365, 1619
Bse3DI	GCAATG	2 cut(s)	301, 754
BseBI	CCWGG	2 cut(s)	1248, 1485
BseCI	ATCGAT	1 cut(s)	1631
BseDI	CCNNGG	7 cut(s)	232, 508, 924, 1426, 1737, 1744, 1927
BseGI	GGATG	3 cut(s)	93, 746, 856
BseLI	CCNNNNNNNGG	2 cut(s)	307, 1516
BseMI	GCAATG	2 cut(s)	301, 754
BseMII	CTCAG	7 cut(s)	369, 768, 1040, 1058, 1092, 1127, 1214
BseNI	ACTGG	6 cut(s)	1010, 1092, 1157, 1183, 1365, 1619
BseRI	GAGGAG	2 cut(s)	768, 1784
BseSI	GKGCMC	1 cut(s)	1287
BseXI	GCAGC	7 cut(s)	92, 175, 197, 203, 904, 1367, 2029
BsgI	GTGCAG	2 cut(s)	1365, 1681
Bsh1236I	CGCG	4 cut(s)	510, 1710, 1834, 1887
BshFI	GGCC	5 cut(s)	8, 61, 923, 1509, 1742
BshNI	GGYRCC	3 cut(s)	310, 1090, 1480
BshVI	ATCGAT	1 cut(s)	1631
BsiHKAI	GWGCWC	1 cut(s)	1287
BsiHKCI	CYCGRG	2 cut(s)	1074, 1926
BsiSI	CCGG	4 cut(s)	23, 308, 2011, 2024
BslFI	GGGAC	2 cut(s)	572, 592
BslI	CCNNNNNNNGG	2 cut(s)	307, 1516
BsmAI	GTCTC	4 cut(s)	119, 160, 1804, 1984
BsmFI	GGGAC	2 cut(s)	572, 592
BsmI	GAATGC	2 cut(s)	1494, 1571
BsnI	GGCC	5 cut(s)	8, 61, 923, 1509, 1742
Bso31I	GGTCTC	1 cut(s)	160
BsoBI	CYCGRG	2 cut(s)	1074, 1926
Bsp1286I	GDGCHC	1 cut(s)	1287
Bsp143I	GATC	6 cut(s)	980, 1371, 1556, 1748, 1858, 1888
Bsp1720I	GCTNAGC	1 cut(s)	1049
Bsp19I	CCATGG	1 cut(s)	924
BspACI	CCGC	8 cut(s)	26, 58, 92, 508, 510, 568, 1885, 1988
BspANI	GGCC	5 cut(s)	8, 61, 923, 1509, 1742
BspCNI	CTCAG	7 cut(s)	370, 769, 1041, 1059, 1093, 1126, 1213
BspDI	ATCGAT	1 cut(s)	1631
BspFNI	CGCG	4 cut(s)	510, 1710, 1834, 1887
BspLI	GGNNCC	6 cut(s)	97, 312, 760, 1092, 1155, 1482
BspMAI	CTGCAG	2 cut(s)	187, 193
BspPI	GGATC	4 cut(s)	988, 1756, 1866, 1883
BspQI	GCTCTTC	1 cut(s)	1599
BspT107I	GGYRCC	3 cut(s)	310, 1090, 1480
BspTNI	GGTCTC	1 cut(s)	160
BsrDI	GCAATG	2 cut(s)	301, 754
BsrFI	RCCGGY	1 cut(s)	307
BsrI	ACTGG	6 cut(s)	1010, 1092, 1157, 1183, 1365, 1619
BssAI	RCCGGY	1 cut(s)	307
BssECI	CCNNGG	7 cut(s)	232, 508, 924, 1426, 1737, 1744, 1927
BssMI	GATC	6 cut(s)	980, 1371, 1556, 1748, 1858, 1888
BssT1I	CCWWGG	5 cut(s)	232, 924, 1426, 1737, 1744
Bst2UI	CCWGG	2 cut(s)	1248, 1485
Bst4CI	ACNGT	3 cut(s)	302, 1496, 1806
Bst6I	CTCTTC	3 cut(s)	881, 1509, 1599
BstC8I	GCNNGC	2 cut(s)	6, 508
BstDEI	CTNAG	8 cut(s)	378, 777, 1049, 1067, 1101, 1113, 1200, 1298
BstDSI	CCRYGG	2 cut(s)	508, 924
BstEII	GGTNACC	1 cut(s)	2018
BstENI	CCTNNNNNAGG	1 cut(s)	1514
BstF5I	GGATG	3 cut(s)	93, 746, 856
BstFNI	CGCG	4 cut(s)	510, 1710, 1834, 1887
BstH2I	RGCGCY	1 cut(s)	907
BstHHI	GCGC	2 cut(s)	906, 1307
BstKTI	GATC	6 cut(s)	983, 1374, 1559, 1751, 1861, 1891
BstMAI	GTCTC	4 cut(s)	119, 160, 1804, 1984
BstMBI	GATC	6 cut(s)	980, 1371, 1556, 1748, 1858, 1888
BstMWI	GCNNNNNNNGC	8 cut(s)	26, 191, 197, 901, 910, 1004, 1106, 1931
BstNI	CCWGG	2 cut(s)	1248, 1485
BstPI	GGTNACC	1 cut(s)	2018
BstSCI	CCNGG	3 cut(s)	1246, 1483, 2022
BstSFI	CTRYAG	4 cut(s)	183, 189, 372, 1598
BstSLI	GKGCMC	1 cut(s)	1287
BstUI	CGCG	4 cut(s)	510, 1710, 1834, 1887
BstV1I	GCAGC	7 cut(s)	92, 175, 197, 203, 904, 1367, 2029
BstV2I	GAAGAC	1 cut(s)	529
BstX2I	RGATCY	2 cut(s)	980, 1556
BstXI	CCANNNNNNTGG	1 cut(s)	84
BstYI	RGATCY	2 cut(s)	980, 1556
Bsu15I	ATCGAT	1 cut(s)	1631
BsuRI	GGCC	5 cut(s)	8, 61, 923, 1509, 1742
BsuTUI	ATCGAT	1 cut(s)	1631
BtgI	CCRYGG	2 cut(s)	508, 924
BtgZI	GCGATG	2 cut(s)	894, 1815
BtrI	CACGTC	1 cut(s)	1910
BtsCI	GGATG	3 cut(s)	93, 746, 856
BtsI	GCAGTG	3 cut(s)	246, 868, 1540
BtsIMutI	CAGTG	7 cut(s)	246, 813, 868, 1064, 1358, 1540, 1818
Cac8I	GCNNGC	2 cut(s)	6, 508
CfoI	GCGC	2 cut(s)	906, 1307
Cfr10I	RCCGGY	1 cut(s)	307
Cfr13I	GGNCC	3 cut(s)	595, 759, 1741
Cfr42I	CCGCGG	1 cut(s)	511
ClaI	ATCGAT	1 cut(s)	1631
CseI	GACGC	1 cut(s)	534
CsiI	ACCWGGT	1 cut(s)	1246
Csp6I	GTAC	5 cut(s)	356, 1091, 1310, 1904, 2036
CviAII	CATG	9 cut(s)	84, 214, 562, 592, 763, 925, 1477, 1862, 2030
CviQI	GTAC	5 cut(s)	356, 1091, 1310, 1904, 2036
DdeI	CTNAG	8 cut(s)	378, 777, 1049, 1067, 1101, 1113, 1200, 1298
DpnI	GATC	6 cut(s)	982, 1373, 1558, 1750, 1860, 1890
DpnII	GATC	6 cut(s)	980, 1371, 1556, 1748, 1858, 1888
DraIII	CACNNNGTG	1 cut(s)	2026
EaeI	YGGCCR	2 cut(s)	6, 59
Eam1104I	CTCTTC	3 cut(s)	881, 1509, 1599
EarI	CTCTTC	3 cut(s)	881, 1509, 1599
EciI	GGCGGA	1 cut(s)	583
Eco130I	CCWWGG	5 cut(s)	232, 924, 1426, 1737, 1744
Eco31I	GGTCTC	1 cut(s)	160
Eco32I	GATATC	1 cut(s)	1231
Eco47I	GGWCC	2 cut(s)	595, 759
Eco57I	CTGAAG	1 cut(s)	905
Eco88I	CYCGRG	2 cut(s)	1074, 1926
Eco91I	GGTNACC	1 cut(s)	2018
EcoNI	CCTNNNNNAGG	1 cut(s)	1514
EcoO65I	GGTNACC	1 cut(s)	2018
EcoRII	CCWGG	2 cut(s)	1246, 1483
EcoRV	GATATC	1 cut(s)	1231
EcoT14I	CCWWGG	5 cut(s)	232, 924, 1426, 1737, 1744
ErhI	CCWWGG	5 cut(s)	232, 924, 1426, 1737, 1744
FaeI	CATG	9 cut(s)	87, 217, 565, 595, 766, 928, 1480, 1865, 2033
FalI	AAGNNNNNCTT	8 cut(s)	706, 738, 1023, 1055, 1506, 1538, 1968, 2000
FaqI	GGGAC	2 cut(s)	572, 592
FatI	CATG	9 cut(s)	83, 213, 561, 591, 762, 924, 1476, 1861, 2029
FauI	CCCGC	4 cut(s)	85, 515, 1892, 1981
FauNDI	CATATG	1 cut(s)	2047
FbaI	TGATCA	1 cut(s)	1371
Fnu4HI	GCNGC	9 cut(s)	27, 59, 106, 186, 189, 192, 893, 1356, 2043
FokI	GGATG	3 cut(s)	100, 753, 863
Fsp4HI	GCNGC	9 cut(s)	27, 59, 106, 186, 189, 192, 893, 1356, 2043
FspBI	CTAG	2 cut(s)	318, 1313
GlaI	GCGC	2 cut(s)	905, 1306
GluI	GCNGC	9 cut(s)	27, 59, 106, 186, 189, 192, 893, 1356, 2043
GsuI	CTGGAG	3 cut(s)	993, 1545, 1956
HaeII	RGCGCY	1 cut(s)	907
HaeIII	GGCC	5 cut(s)	8, 61, 923, 1509, 1742
HapII	CCGG	4 cut(s)	23, 308, 2011, 2024
HgaI	GACGC	1 cut(s)	534
HhaI	GCGC	2 cut(s)	906, 1307
Hin1II	CATG	9 cut(s)	87, 217, 565, 595, 766, 928, 1480, 1865, 2033
Hin6I	GCGC	2 cut(s)	904, 1305
HinP1I	GCGC	2 cut(s)	904, 1305
HincII	GTYRAC	2 cut(s)	34, 305
HindII	GTYRAC	2 cut(s)	34, 305
HindIII	AAGCTT	1 cut(s)	198
HinfI	GANTC	8 cut(s)	80, 536, 704, 1069, 1451, 1755, 1839, 1941
HpaII	CCGG	4 cut(s)	23, 308, 2011, 2024
HphI	GGTGA	3 cut(s)	1562, 1768, 2012
Hpy166II	GTNNAC	4 cut(s)	34, 305, 1285, 1776
Hpy188I	TCNGA	9 cut(s)	129, 165, 393, 643, 679, 885, 1203, 1266, 1946
Hpy188III	TCNNGA	5 cut(s)	343, 468, 1460, 1562, 1752
Hpy8I	GTNNAC	4 cut(s)	34, 305, 1285, 1776
Hpy99I	CGWCG	1 cut(s)	1911
HpyAV	CCTTC	5 cut(s)	396, 474, 568, 795, 1561
HpyCH4III	ACNGT	3 cut(s)	302, 1496, 1806
HpyCH4IV	ACGT	5 cut(s)	956, 1418, 1658, 1687, 1909
HpyF10VI	GCNNNNNNNGC	8 cut(s)	26, 191, 197, 901, 910, 1004, 1106, 1931
HpyF3I	CTNAG	8 cut(s)	378, 777, 1049, 1067, 1101, 1113, 1200, 1298
HpySE526I	ACGT	5 cut(s)	956, 1418, 1658, 1687, 1909
Hsp92II	CATG	9 cut(s)	87, 217, 565, 595, 766, 928, 1480, 1865, 2033
HspAI	GCGC	2 cut(s)	904, 1305
KpnI	GGTACC	1 cut(s)	1094
Ksp22I	TGATCA	1 cut(s)	1371
KspI	CCGCGG	1 cut(s)	511
Kzo9I	GATC	6 cut(s)	980, 1371, 1556, 1748, 1858, 1888
LguI	GCTCTTC	1 cut(s)	1599
LmnI	GCTCC	4 cut(s)	25, 715, 940, 1012
Lsp1109I	GCAGC	7 cut(s)	92, 175, 197, 203, 904, 1367, 2029
LweI	GCATC	4 cut(s)	430, 493, 1292, 1838
MabI	ACCWGGT	1 cut(s)	1246
MaeI	CTAG	2 cut(s)	318, 1313
MaeII	ACGT	5 cut(s)	956, 1418, 1658, 1687, 1909
MaeIII	GTNAC	5 cut(s)	1174, 1387, 1408, 1419, 2018
MalI	GATC	6 cut(s)	982, 1373, 1558, 1750, 1860, 1890
MboI	GATC	6 cut(s)	980, 1371, 1556, 1748, 1858, 1888
MboII	GAAGA	9 cut(s)	113, 534, 714, 898, 1043, 1046, 1526, 1586, 1929
MflI	RGATCY	2 cut(s)	980, 1556
MhlI	GDGCHC	1 cut(s)	1287
MlsI	TGGCCA	1 cut(s)	8
MluNI	TGGCCA	1 cut(s)	8
MlyI	GAGTC	2 cut(s)	1078, 1749
MmeI	TCCRAC	1 cut(s)	1894
Mox20I	TGGCCA	1 cut(s)	8
MroXI	GAANNNNTTC	3 cut(s)	1038, 1455, 1571
MscI	TGGCCA	1 cut(s)	8
MseI	TTAA	6 cut(s)	396, 1025, 1269, 1335, 1682, 2062
MslI	CAYNNNNRTG	1 cut(s)	1961
Msp20I	TGGCCA	1 cut(s)	8
MspA1I	CMGCKG	4 cut(s)	105, 188, 510, 2042
MspI	CCGG	4 cut(s)	23, 308, 2011, 2024
MspR9I	CCNGG	3 cut(s)	1248, 1485, 2024
Mva1269I	GAATGC	2 cut(s)	1494, 1571
MvaI	CCWGG	2 cut(s)	1248, 1485
MvnI	CGCG	4 cut(s)	510, 1710, 1834, 1887
MwoI	GCNNNNNNNGC	8 cut(s)	26, 191, 197, 901, 910, 1004, 1106, 1931
NciI	CCSGG	1 cut(s)	2024
NcoI	CCATGG	1 cut(s)	924
NdeI	CATATG	1 cut(s)	2047
NdeII	GATC	6 cut(s)	980, 1371, 1556, 1748, 1858, 1888
NlaIII	CATG	9 cut(s)	87, 217, 565, 595, 766, 928, 1480, 1865, 2033
NlaIV	GGNNCC	6 cut(s)	97, 312, 760, 1092, 1155, 1482
NmuCI	GTSAC	2 cut(s)	1408, 2018
PaeR7I	CTCGAG	1 cut(s)	1074
PciSI	GCTCTTC	1 cut(s)	1599
PcsI	WCGNNNNNNNCGW	1 cut(s)	225
PctI	GAATGC	2 cut(s)	1494, 1571
PdmI	GAANNNNTTC	3 cut(s)	1038, 1455, 1571
PfeI	GAWTC	6 cut(s)	80, 536, 704, 1451, 1839, 1941
PkrI	GCNGC	9 cut(s)	28, 60, 107, 187, 190, 193, 894, 1357, 2044
PleI	GAGTC	2 cut(s)	1077, 1749
PpsI	GAGTC	2 cut(s)	1077, 1749
Psp1406I	AACGTT	1 cut(s)	1418
Psp6I	CCWGG	2 cut(s)	1246, 1483
PspEI	GGTNACC	1 cut(s)	2018
PspGI	CCWGG	2 cut(s)	1246, 1483
PspN4I	GGNNCC	6 cut(s)	97, 312, 760, 1092, 1155, 1482
PspPI	GGNCC	3 cut(s)	595, 759, 1741
PspXI	VCTCGAGB	1 cut(s)	1074
PstI	CTGCAG	2 cut(s)	187, 193
PsuI	RGATCY	2 cut(s)	980, 1556
PvuII	CAGCTG	3 cut(s)	105, 188, 2042
RsaI	GTAC	5 cut(s)	357, 1092, 1311, 1905, 2037
RsaNI	GTAC	5 cut(s)	356, 1091, 1310, 1904, 2036
RseI	CAYNNNNRTG	1 cut(s)	1961
SacII	CCGCGG	1 cut(s)	511
SapI	GCTCTTC	1 cut(s)	1599
SaqAI	TTAA	6 cut(s)	396, 1025, 1269, 1335, 1682, 2062
SatI	GCNGC	9 cut(s)	27, 59, 106, 186, 189, 192, 893, 1356, 2043
Sau3AI	GATC	6 cut(s)	980, 1371, 1556, 1748, 1858, 1888
Sau96I	GGNCC	3 cut(s)	595, 759, 1741
SchI	GAGTC	2 cut(s)	1078, 1749
ScrFI	CCNGG	3 cut(s)	1248, 1485, 2024
SduI	GDGCHC	1 cut(s)	1287
SexAI	ACCWGGT	1 cut(s)	1246
SfaNI	GCATC	4 cut(s)	430, 493, 1292, 1838
SfcI	CTRYAG	4 cut(s)	183, 189, 372, 1598
Sfr274I	CTCGAG	1 cut(s)	1074
Sfr303I	CCGCGG	1 cut(s)	511
SgrBI	CCGCGG	1 cut(s)	511
SinI	GGWCC	2 cut(s)	595, 759
SlaI	CTCGAG	1 cut(s)	1074
SmiMI	CAYNNNNRTG	1 cut(s)	1961
SmlI	CTYRAG	2 cut(s)	529, 1074
SmoI	CTYRAG	2 cut(s)	529, 1074
SpeI	ACTAGT	1 cut(s)	317
SsiI	CCGC	8 cut(s)	26, 58, 92, 508, 510, 568, 1885, 1988
SspMI	CTAG	2 cut(s)	318, 1313
StyD4I	CCNGG	3 cut(s)	1246, 1483, 2022
StyI	CCWWGG	5 cut(s)	232, 924, 1426, 1737, 1744
TaaI	ACNGT	3 cut(s)	302, 1496, 1806
TaiI	ACGT	5 cut(s)	959, 1421, 1661, 1690, 1912
TaqI	TCGA	7 cut(s)	289, 545, 835, 1042, 1075, 1631, 1842
TatI	WGTACW	2 cut(s)	355, 1309
TauI	GCSGC	2 cut(s)	29, 61
TfiI	GAWTC	6 cut(s)	80, 536, 704, 1451, 1839, 1941
Tru1I	TTAA	6 cut(s)	396, 1025, 1269, 1335, 1682, 2062
Tru9I	TTAA	6 cut(s)	396, 1025, 1269, 1335, 1682, 2062
TscAI	CASTG	7 cut(s)	253, 820, 868, 1071, 1365, 1540, 1825
TseFI	GTSAC	2 cut(s)	1408, 2018
TseI	GCWGC	7 cut(s)	105, 185, 188, 191, 892, 1355, 2042
Tsp45I	GTSAC	2 cut(s)	1408, 2018
TspDTI	ATGAA	5 cut(s)	113, 214, 426, 438, 1945
TspGWI	ACGGA	1 cut(s)	632
TspRI	CASTG	7 cut(s)	253, 820, 868, 1071, 1365, 1540, 1825
VneI	GTGCAC	1 cut(s)	1283
VpaK11BI	GGWCC	2 cut(s)	595, 759
XagI	CCTNNNNNAGG	1 cut(s)	1514
XapI	RAATTY	5 cut(s)	476, 551, 806, 1145, 1431
XcmI	CCANNNNNNNNNTGG	2 cut(s)	769, 980
XhoI	CTCGAG	1 cut(s)	1074
XmnI	GAANNNNTTC	3 cut(s)	1038, 1455, 1571
XspI	CTAG	2 cut(s)	318, 1313

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.