Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004471

Physical Location & Seq

Genomic Coordinates Reverse (-)

16037646 .. 16041848

Gene Structure 4203 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1186000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1143 bp

ATGCTAATAAAACATGGAATAAATTCTGGGTTTGTTGCAAGAACTTGCCTTTACATGTTGGGTTCCTCTGGTGTGCTGAGAAGGCAACAGTTTCTGATAAGGTCTCTCGGAGTAGGAGGTTGCAGTGATCGTAACACCATCACAAATCAGAAGGCAATGCAAGGAATTAGGAAATTCACGAGTCCGCCCTCTTTGGAAGCCGACGCCGAGAGCGTTTTGCGAGCAATCACTCCGACCCTCGATCCCAACAGACACAAGGGTCAGGCTGGAAAGATAGCTGTTATCGGCGGCTGTCGTGAGTACACCGGTGCTCCATACTTTTCTGCTATCTCAGCATTAAAAATTGGTGCAGACCTGTCCCATGTCTTTTGTACAAAAGATGCAGCTTCGGTCATAAAAAGCTACAGTCCTGAGTTGATTGTACACCCTATTTTAGAAGAATCGTACAATGTTAGGGATGAGGACAGAAGTTTCATCTCAGAAAAGGTTCTTGCTGAAGTTGATAAATGGATGGAAAGATTTGACTGTCTTGTCGTTGGTCCAGGCCTCGGAAGGGACCCATTTCTTCTGGACTGTGTGAGCAACATCATGAAGCATGCAAGGCGGTCCAATGTCCCAATTGTCATTGATGGGGATGGACTCTTTATGGTCACAAACTGTATTGATCTTGTCAGTGGATATCCCTTAGCTGTGCTGACCCCAAACATAAATGAATATAAGCGCCTTGTACAGAAAGTTTTGAGCTGTGAAGTAAATGATGAAGATGCTCCCGAGCAAGTACTATCTCTTGCAAAAAGGATTGGTGGTGTAACCATACTTAGGAAAGGAAGATCTGACCTCATTAGTGATGGTGAGACAGTCAACTCAGTGAGCATATACGGTTCTCCTCGACGATGTGGTGGGCAGGGTGATATACTTTCTGGAAGCGTTGGTGTATTTCTATCATGGGCCCGTCAAAAGATCAAAGATGGGGATTCGAGTACCAGTTCCAGAAATCCTGCACTGTTGGGGTGCATTGCTGCGTCCGCTTTAATGAGGAAGGCTGCATCCCTTGCTTTTGAGAATAAGAAAAGGTCAACCCTCACTACCGATATCATCGAGTGCTTGGGAAGAAGTTTGGAGGATATCTGTCCAGTTGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006734 GO:0006739 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009117 GO:0009507 GO:0009536 GO:0009987 GO:0016829 GO:0016835 GO:0016836 GO:0016853 GO:0016854 GO:0019362 GO:0019637 GO:0019674 GO:0034641 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046496 GO:0047453 GO:0051186 GO:0052856 GO:0052857 GO:0055086 GO:0071704 GO:0072524 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Carb_kinase

Protein Analysis

381

Amino Acids

41.11

Weight (kDa)

8.54

Isoelectric Point (pI)

47.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Carb_kinase	PF01256	93 - 368	1.5e-47	NAD(P)H-hydrate dehydratase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004471)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19150 AT5G19150
fragaria_vesca	FvH4_4g14760 FvH4_4g14760 FvH4_4g14760 FvH4_4g14760
malus_domestica	MD16G1186000.v1.1
prunus_persica	Prupe.1G152800_v2.0.a1 Prupe.1G152800_v2.0.a1 Prupe.1G152800_v2.0.a1 Prupe.1G152800_v2.0.a1 Prupe.1G152800_v2.0.a1 Prupe.1G152800_v2.0.a1
pyrus_communis	pycom16g15570
rosa_chinensis	RchiOBHm_Chr0c32g0501471 RchiOBHm_Chr0c32g0501661 RchiOBHm_Chr3g0483801 RchiOBHm_Chr4g0417841
rosa_laevigata	RLG00000007895 RLG00000023259
rosa_multiflora	Rmu_sc0001333.1_g000001
rosa_roxburghii	Rroxscaffold_5G00361250 Rroxscaffold_6G00398130
rosa_rugosa	Rorug04G0149500
rosa_samantha	Rh3AG256800 Rh3CG291600 Rh4AG212000 Rh4BG219000 Rh4CG222700 Rh4DG209800
rosa_wichuraiana	Rw3G023200 Rw4G017970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	258, 530
AciI	CCGC	4 cut(s)	185, 288, 604, 1026
AclWI	GGATC	1 cut(s)	236
AcsI	RAATTY	2 cut(s)	22, 173
AcuI	CTGAAG	1 cut(s)	516
AcyI	GRCGYC	1 cut(s)	204
AfaI	GTAC	7 cut(s)	302, 373, 423, 446, 729, 780, 982
AfiI	CCNNNNNNNGG	3 cut(s)	548, 553, 819
AflIII	ACRYGT	1 cut(s)	54
AgeI	ACCGGT	1 cut(s)	305
AjnI	CCWGG	1 cut(s)	541
AluBI	AGCT	5 cut(s)	278, 386, 402, 689, 744
AluI	AGCT	5 cut(s)	278, 386, 402, 689, 744
Alw21I	GWGCWC	1 cut(s)	313
Alw26I	GTCTC	2 cut(s)	108, 848
AlwI	GGATC	1 cut(s)	236
AlwNI	CAGNNNCTG	1 cut(s)	94
Ama87I	CYCGRG	1 cut(s)	770
AoxI	GGCC	2 cut(s)	544, 948
ApaI	GGGCCC	1 cut(s)	952
ApeKI	GCWGC	3 cut(s)	383, 1019, 1043
ApoI	RAATTY	2 cut(s)	22, 173
AsiGI	ACCGGT	1 cut(s)	305
Asp700I	GAANNNNTTC	2 cut(s)	22, 486
AspLEI	GCGC	1 cut(s)	723
AspS9I	GGNCC	5 cut(s)	539, 556, 606, 948, 949
AsuHPI	GGTGA	2 cut(s)	863, 920
AvaI	CYCGRG	1 cut(s)	770
AvaII	GGWCC	3 cut(s)	539, 556, 606
BaeGI	GKGCMC	1 cut(s)	952
BanII	GRGCYC	1 cut(s)	952
BauI	CACGAG	1 cut(s)	178
Bbv12I	GWGCWC	1 cut(s)	313
BbvI	GCAGC	3 cut(s)	395, 1006, 1030
BccI	CCATC	6 cut(s)	146, 505, 623, 629, 842, 962
BciT130I	CCWGG	1 cut(s)	543
BcoDI	GTCTC	2 cut(s)	108, 848
BfmI	CTRYAG	1 cut(s)	403
BfoI	RGCGCY	1 cut(s)	724
BglII	AGATCT	1 cut(s)	830
BisI	GCNGC	4 cut(s)	289, 384, 1020, 1044
BlsI	GCNGC	4 cut(s)	290, 385, 1021, 1045
BmcAI	AGTACT	1 cut(s)	780
Bme1390I	CCNGG	1 cut(s)	543
Bme18I	GGWCC	3 cut(s)	539, 556, 606
BmeT110I	CYCGRG	1 cut(s)	770
BmgT120I	GGNCC	5 cut(s)	539, 556, 606, 948, 949
BmiI	GGNNCC	4 cut(s)	64, 557, 558, 950
BmrFI	CCNGG	1 cut(s)	543
BmsI	GCATC	3 cut(s)	370, 754, 1055
Bpu10I	CCTNAGC	1 cut(s)	685
BsaHI	GRCGYC	1 cut(s)	204
BsaI	GGTCTC	1 cut(s)	108
BsaJI	CCNNGG	1 cut(s)	547
BsaWI	WCCGGW	1 cut(s)	305
BsaXI	ACNNNNNCTCC	2 cut(s)	295, 325
Bsc4I	CCNNNNNNNGG	3 cut(s)	548, 553, 819
Bse118I	RCCGGY	1 cut(s)	305
Bse1I	ACTGG	2 cut(s)	984, 1133
Bse3DI	GCAATG	2 cut(s)	162, 1014
BseBI	CCWGG	1 cut(s)	543
BseDI	CCNNGG	1 cut(s)	547
BseGI	GGATG	4 cut(s)	463, 516, 640, 1046
BseLI	CCNNNNNNNGG	3 cut(s)	548, 553, 819
BseMI	GCAATG	2 cut(s)	162, 1014
BseMII	CTCAG	5 cut(s)	68, 345, 402, 492, 879
BseNI	ACTGG	2 cut(s)	984, 1133
BseRI	GAGGAG	1 cut(s)	876
BseSI	GKGCMC	1 cut(s)	952
BseXI	GCAGC	3 cut(s)	395, 1006, 1030
BsgI	GTGCAG	2 cut(s)	369, 984
BshFI	GGCC	2 cut(s)	546, 950
BshTI	ACCGGT	1 cut(s)	305
BsiHKAI	GWGCWC	1 cut(s)	313
BsiHKCI	CYCGRG	1 cut(s)	770
BsiSI	CCGG	1 cut(s)	306
BslFI	GGGAC	3 cut(s)	343, 569, 599
BslI	CCNNNNNNNGG	3 cut(s)	548, 553, 819
BsmAI	GTCTC	2 cut(s)	108, 848
BsmFI	GGGAC	3 cut(s)	343, 569, 599
BsnI	GGCC	2 cut(s)	546, 950
Bso31I	GGTCTC	1 cut(s)	108
BsoBI	CYCGRG	1 cut(s)	770
Bsp120I	GGGCCC	1 cut(s)	948
Bsp1286I	GDGCHC	2 cut(s)	313, 952
Bsp1407I	TGTACA	3 cut(s)	371, 421, 727
Bsp143I	GATC	5 cut(s)	127, 241, 664, 830, 960
BspACI	CCGC	4 cut(s)	185, 288, 604, 1026
BspANI	GGCC	2 cut(s)	546, 950
BspCNI	CTCAG	5 cut(s)	69, 344, 403, 491, 878
BspHI	TCATGA	1 cut(s)	588
BspLI	GGNNCC	4 cut(s)	64, 557, 558, 950
BspPI	GGATC	1 cut(s)	236
BspTNI	GGTCTC	1 cut(s)	108
BsrDI	GCAATG	2 cut(s)	162, 1014
BsrFI	RCCGGY	1 cut(s)	305
BsrGI	TGTACA	3 cut(s)	371, 421, 727
BsrI	ACTGG	2 cut(s)	984, 1133
BssAI	RCCGGY	1 cut(s)	305
BssECI	CCNNGG	1 cut(s)	547
BssMI	GATC	5 cut(s)	127, 241, 664, 830, 960
BssNI	GRCGYC	1 cut(s)	204
BssSI	CACGAG	1 cut(s)	178
Bst2BI	CACGAG	1 cut(s)	178
Bst2UI	CCWGG	1 cut(s)	543
Bst4CI	ACNGT	8 cut(s)	90, 407, 527, 575, 659, 859, 881, 1005
BstACI	GRCGYC	1 cut(s)	204
BstAPI	GCANNNNNTGC	1 cut(s)	1052
BstAUI	TGTACA	3 cut(s)	371, 421, 727
BstC8I	GCNNGC	2 cut(s)	222, 597
BstDEI	CTNAG	7 cut(s)	77, 331, 411, 478, 685, 818, 865
BstF5I	GGATG	4 cut(s)	463, 516, 640, 1046
BstH2I	RGCGCY	1 cut(s)	724
BstHHI	GCGC	1 cut(s)	723
BstKTI	GATC	5 cut(s)	130, 244, 667, 833, 963
BstMAI	GTCTC	2 cut(s)	108, 848
BstMBI	GATC	5 cut(s)	127, 241, 664, 830, 960
BstMWI	GCNNNNNNNGC	5 cut(s)	82, 332, 601, 1025, 1052
BstNI	CCWGG	1 cut(s)	543
BstNSI	RCATGY	2 cut(s)	58, 599
BstSCI	CCNGG	1 cut(s)	541
BstSFI	CTRYAG	1 cut(s)	403
BstSLI	GKGCMC	1 cut(s)	952
BstV1I	GCAGC	3 cut(s)	395, 1006, 1030
BstX2I	RGATCY	1 cut(s)	830
BstYI	RGATCY	1 cut(s)	830
BsuRI	GGCC	2 cut(s)	546, 950
BtsCI	GGATG	4 cut(s)	463, 516, 640, 1046
BtsI	GCAGTG	1 cut(s)	130
BtsIMutI	CAGTG	4 cut(s)	130, 679, 873, 1001
Cac8I	GCNNGC	2 cut(s)	222, 597
CaiI	CAGNNNCTG	1 cut(s)	94
CciI	TCATGA	1 cut(s)	588
CfoI	GCGC	1 cut(s)	723
Cfr10I	RCCGGY	1 cut(s)	305
Cfr13I	GGNCC	5 cut(s)	539, 556, 606, 948, 949
CseI	GACGC	2 cut(s)	212, 1011
Csp6I	GTAC	7 cut(s)	301, 372, 422, 445, 728, 779, 981
CspAI	ACCGGT	1 cut(s)	305
CviAII	CATG	6 cut(s)	14, 55, 362, 589, 596, 945
CviQI	GTAC	7 cut(s)	301, 372, 422, 445, 728, 779, 981
DdeI	CTNAG	7 cut(s)	77, 331, 411, 478, 685, 818, 865
DpnI	GATC	5 cut(s)	129, 243, 666, 832, 962
DpnII	GATC	5 cut(s)	127, 241, 664, 830, 960
DrdI	GACNNNNNNGTC	2 cut(s)	258, 530
DseDI	GACNNNNNNGTC	2 cut(s)	258, 530
EciI	GGCGGA	1 cut(s)	174
Eco147I	AGGCCT	1 cut(s)	546
Eco24I	GRGCYC	1 cut(s)	952
Eco31I	GGTCTC	1 cut(s)	108
Eco32I	GATATC	3 cut(s)	680, 1093, 1126
Eco47I	GGWCC	3 cut(s)	539, 556, 606
Eco57I	CTGAAG	1 cut(s)	516
Eco88I	CYCGRG	1 cut(s)	770
EcoO109I	RGGNCCY	1 cut(s)	556
EcoRII	CCWGG	1 cut(s)	541
EcoRV	GATATC	3 cut(s)	680, 1093, 1126
EcoT38I	GRGCYC	1 cut(s)	952
FaeI	CATG	6 cut(s)	17, 58, 365, 592, 599, 948
FaqI	GGGAC	3 cut(s)	343, 569, 599
FatI	CATG	6 cut(s)	13, 54, 361, 588, 595, 944
Fnu4HI	GCNGC	4 cut(s)	289, 384, 1020, 1044
FokI	GGATG	4 cut(s)	470, 523, 647, 1033
FriOI	GRGCYC	1 cut(s)	952
Fsp4HI	GCNGC	4 cut(s)	289, 384, 1020, 1044
GlaI	GCGC	1 cut(s)	722
GluI	GCNGC	4 cut(s)	289, 384, 1020, 1044
HaeII	RGCGCY	1 cut(s)	724
HaeIII	GGCC	2 cut(s)	546, 950
HapII	CCGG	1 cut(s)	306
HgaI	GACGC	2 cut(s)	212, 1011
HhaI	GCGC	1 cut(s)	723
Hin1I	GRCGYC	1 cut(s)	204
Hin1II	CATG	6 cut(s)	17, 58, 365, 592, 599, 948
Hin6I	GCGC	1 cut(s)	721
HinP1I	GCGC	1 cut(s)	721
HincII	GTYRAC	2 cut(s)	862, 1077
HindII	GTYRAC	2 cut(s)	862, 1077
HinfI	GANTC	4 cut(s)	181, 440, 639, 974
HpaII	CCGG	1 cut(s)	306
HphI	GGTGA	2 cut(s)	863, 920
Hpy166II	GTNNAC	4 cut(s)	303, 424, 862, 1077
Hpy188I	TCNGA	7 cut(s)	96, 110, 150, 234, 481, 551, 835
Hpy188III	TCNNGA	8 cut(s)	178, 296, 410, 569, 589, 770, 921, 990
Hpy8I	GTNNAC	4 cut(s)	303, 424, 862, 1077
Hpy99I	CGWCG	2 cut(s)	206, 894
HpyAV	CCTTC	4 cut(s)	75, 145, 546, 1033
HpyCH4III	ACNGT	8 cut(s)	90, 407, 527, 575, 659, 859, 881, 1005
HpyF10VI	GCNNNNNNNGC	5 cut(s)	82, 332, 601, 1025, 1052
HpyF3I	CTNAG	7 cut(s)	77, 331, 411, 478, 685, 818, 865
Hsp92I	GRCGYC	1 cut(s)	204
Hsp92II	CATG	6 cut(s)	17, 58, 365, 592, 599, 948
HspAI	GCGC	1 cut(s)	721
KflI	GGGWCCC	1 cut(s)	556
Kzo9I	GATC	5 cut(s)	127, 241, 664, 830, 960
LmnI	GCTCC	2 cut(s)	316, 772
Lsp1109I	GCAGC	3 cut(s)	395, 1006, 1030
LweI	GCATC	3 cut(s)	370, 754, 1055
MaeIII	GTNAC	3 cut(s)	131, 649, 808
MalI	GATC	5 cut(s)	129, 243, 666, 832, 962
MboI	GATC	5 cut(s)	127, 241, 664, 830, 960
MboII	GAAGA	5 cut(s)	449, 557, 773, 840, 1122
MfeI	CAATTG	1 cut(s)	618
MflI	RGATCY	1 cut(s)	830
MhlI	GDGCHC	2 cut(s)	313, 952
MluCI	AATT	5 cut(s)	22, 165, 173, 342, 618
MlyI	GAGTC	2 cut(s)	190, 633
MmeI	TCCRAC	1 cut(s)	257
MroXI	GAANNNNTTC	2 cut(s)	22, 486
MseI	TTAA	2 cut(s)	338, 1031
MspI	CCGG	1 cut(s)	306
MspR9I	CCNGG	1 cut(s)	543
MunI	CAATTG	1 cut(s)	618
MvaI	CCWGG	1 cut(s)	543
MwoI	GCNNNNNNNGC	5 cut(s)	82, 332, 601, 1025, 1052
NdeII	GATC	5 cut(s)	127, 241, 664, 830, 960
NlaIII	CATG	6 cut(s)	17, 58, 365, 592, 599, 948
NlaIV	GGNNCC	4 cut(s)	64, 557, 558, 950
NmeAIII	GCCGAG	1 cut(s)	232
NmuCI	GTSAC	1 cut(s)	649
NspI	RCATGY	2 cut(s)	58, 599
PaeI	GCATGC	1 cut(s)	599
PagI	TCATGA	1 cut(s)	588
PceI	AGGCCT	1 cut(s)	546
PciI	ACATGT	1 cut(s)	54
PcsI	WCGNNNNNNNCGW	1 cut(s)	210
PdmI	GAANNNNTTC	2 cut(s)	22, 486
PfeI	GAWTC	2 cut(s)	440, 974
PinAI	ACCGGT	1 cut(s)	305
PkrI	GCNGC	4 cut(s)	290, 385, 1021, 1045
PleI	GAGTC	2 cut(s)	189, 633
PpsI	GAGTC	2 cut(s)	189, 633
PpuMI	RGGWCCY	1 cut(s)	556
PscI	ACATGT	1 cut(s)	54
Psp5II	RGGWCCY	1 cut(s)	556
Psp6I	CCWGG	1 cut(s)	541
PspGI	CCWGG	1 cut(s)	541
PspN4I	GGNNCC	4 cut(s)	64, 557, 558, 950
PspOMI	GGGCCC	1 cut(s)	948
PspPI	GGNCC	5 cut(s)	539, 556, 606, 948, 949
PspPPI	RGGWCCY	1 cut(s)	556
PstNI	CAGNNNCTG	1 cut(s)	94
PsuI	RGATCY	1 cut(s)	830
RsaI	GTAC	7 cut(s)	302, 373, 423, 446, 729, 780, 982
RsaNI	GTAC	7 cut(s)	301, 372, 422, 445, 728, 779, 981
SaqAI	TTAA	2 cut(s)	338, 1031
SatI	GCNGC	4 cut(s)	289, 384, 1020, 1044
Sau3AI	GATC	5 cut(s)	127, 241, 664, 830, 960
Sau96I	GGNCC	5 cut(s)	539, 556, 606, 948, 949
ScaI	AGTACT	1 cut(s)	780
SchI	GAGTC	2 cut(s)	190, 633
ScrFI	CCNGG	1 cut(s)	543
SduI	GDGCHC	2 cut(s)	313, 952
SfaNI	GCATC	3 cut(s)	370, 754, 1055
SfcI	CTRYAG	1 cut(s)	403
SgrAI	CRCCGGYG	1 cut(s)	305
SinI	GGWCC	3 cut(s)	539, 556, 606
SphI	GCATGC	1 cut(s)	599
Sse9I	AATT	5 cut(s)	22, 165, 173, 342, 618
SseBI	AGGCCT	1 cut(s)	546
SsiI	CCGC	4 cut(s)	185, 288, 604, 1026
StuI	AGGCCT	1 cut(s)	546
StyD4I	CCNGG	1 cut(s)	541
TaaI	ACNGT	8 cut(s)	90, 407, 527, 575, 659, 859, 881, 1005
TaqI	TCGA	4 cut(s)	240, 889, 977, 1098
TaqII	GACCGA	1 cut(s)	379
TasI	AATT	5 cut(s)	22, 165, 173, 342, 618
TatI	WGTACW	5 cut(s)	300, 371, 421, 727, 778
TauI	GCSGC	1 cut(s)	291
TfiI	GAWTC	2 cut(s)	440, 974
Tru1I	TTAA	2 cut(s)	338, 1031
Tru9I	TTAA	2 cut(s)	338, 1031
TscAI	CASTG	4 cut(s)	130, 679, 873, 1008
TseFI	GTSAC	1 cut(s)	649
TseI	GCWGC	3 cut(s)	383, 1019, 1043
Tsp45I	GTSAC	1 cut(s)	649
TspDTI	ATGAA	4 cut(s)	463, 605, 726, 774
TspRI	CASTG	4 cut(s)	130, 679, 873, 1008
VpaK11BI	GGWCC	3 cut(s)	539, 556, 606
XapI	RAATTY	2 cut(s)	22, 173
XceI	RCATGY	2 cut(s)	58, 599
XmnI	GAANNNNTTC	2 cut(s)	22, 486
ZrmI	AGTACT	1 cut(s)	780

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.