RLG00000007895

Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004471
Physical Location & Seq
Reverse (-)
22557073 .. 22560837
3765 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007895

Sequence Viewer

Length: 1113 bp
ATGTTTGTAAAACTGGGAGTGAATTCTGGGTTTGGTGCAAAGAGTTGTATTTGTATGCTGGCTTCTTCAGCTGTGTTGAGAAGGCAACAGTTGGTGATAAGGTGTCTTGGAGTTGGAGGTTACAGTGATCATAACATTAACACTAATCAGAAACCAATGCAAGGAGTTAGATTCAGTAGTGGAGCTTCTTTGGAGGCTGATGCTGAGAATGTTCTGAGAGCAATCACACCGACCCTTGATCCAAATAGACACAAGGGTCAGGCTGGAAAGATAGCTGTTATTGGTGGGTGCCGTGAGTACACTGGTGCTCCATATTTTGCTGCTATCTCAGCATTAAAAATTGGTGTGGATCTGTCCCATGTTTTCTGTACAAGAGATGCTGCTTCAGTTATAAAAAGCTACAGTCCTGAGTTGATCGTACACCCAATTTTAGAAGAGTCATACAGTGTTAGGGATGAGGACAAGAGCTCTATCTTAGAAAAGGCTCTTGGTGAAGTTGATAAGTGGATGGAAAGATTCGATTGTCTTGTAATTGGTCCGGGCCTTGGAAGGGACCCATTTCTTCTGGATTGTGTTAGCAAAATCATAAAGCATGCAAGACAGTCCAATGTTCCAATTGTCATAGATGGGGATGGACTCTTTCTTATTACAAACTGTCTTGATCTTGTAAGTGGATATCACTTGGCTGTCCTGACTCCAAATGTAAATGAATACAAGCGACTTGTACAAAAAGTTTTGAGTTGTGAAGTAAATGATGAACATGCACGTGAGCAATTACTATCTCTTGCAAAAAGGATTGGTGGTGTCACTATACTTAGGAAAGGAAAAGCTGACCTCATTAGTGATGGTGAGACAGAATGCATTACCCAAACATCCATGCGCATGTGTGGGAATCGCGTTGCTGTATTTCTTTCATGGGCCCGTCAAATTGTCACAGATGAGAATCTGAGTATCAGTTCCCGAAACCCCGCAATGTTGGGTTGCATTGCTGGCTCCACTTTAATGAGGAAGGCTGCATCCCTGGCTTTTGAGAATAAGAAAAGGTCAACTCTTACTACTGACATCATAGAATGCTTGGGAAGAAGTTTGCAGGATATTTGTCCAGTTCCCTGA

Protein Analysis

371

Amino Acids

40.26

Weight (kDa)

8.34

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_kinase PF01256 92 - 357 2.7e-36 NAD(P)H-hydrate dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 392
AasI GACNNNNNNGTC 1 cut(s) 255
Acc16I TGCGCA 1 cut(s) 881
AccB1I GGYRCC 1 cut(s) 288
AccII CGCG 1 cut(s) 897
AciI CCGC 1 cut(s) 969
AclWI GGATC 2 cut(s) 233, 357
AcsI RAATTY 1 cut(s) 22
AcuI CTGAAG 2 cut(s) 51, 369
AcvI CACGTG 1 cut(s) 767
AfaI GTAC 4 cut(s) 299, 370, 420, 726
AfiI CCNNNNNNNGG 3 cut(s) 161, 545, 550
AjnI CCWGG 1 cut(s) 1020
AjuI GAANNNNNNNTTGG 2 cut(s) 471, 503
AluBI AGCT 6 cut(s) 71, 185, 275, 399, 468, 830
AluI AGCT 6 cut(s) 71, 185, 275, 399, 468, 830
Alw21I GWGCWC 2 cut(s) 310, 470
Alw26I GTCTC 1 cut(s) 845
AlwI GGATC 2 cut(s) 233, 357
AoxI GGCC 2 cut(s) 541, 918
ApaI GGGCCC 1 cut(s) 922
ApeKI GCWGC 3 cut(s) 320, 380, 1013
ApoI RAATTY 1 cut(s) 22
AspLEI GCGC 1 cut(s) 882
AspS9I GGNCC 5 cut(s) 536, 541, 553, 918, 919
AsuC2I CCSGG 1 cut(s) 540
AsuHPI GGTGA 3 cut(s) 106, 503, 860
AvaII GGWCC 2 cut(s) 536, 553
BaeGI GKGCMC 1 cut(s) 922
BanI GGYRCC 1 cut(s) 288
BanII GRGCYC 2 cut(s) 470, 922
BarI GAAGNNNNNNTAC 4 cut(s) 46, 78, 169, 201
BbrPI CACGTG 1 cut(s) 767
Bbv12I GWGCWC 2 cut(s) 310, 470
BbvI GCAGC 3 cut(s) 307, 367, 1000
BccI CCATC 4 cut(s) 502, 620, 626, 839
BceAI ACGGC 1 cut(s) 276
BciT130I CCWGG 1 cut(s) 1022
BclI TGATCA 1 cut(s) 127
BcnI CCSGG 1 cut(s) 540
BcoDI GTCTC 1 cut(s) 845
BfmI CTRYAG 1 cut(s) 400
BisI GCNGC 3 cut(s) 321, 381, 1014
BlsI GCNGC 3 cut(s) 322, 382, 1015
Bme1390I CCNGG 2 cut(s) 540, 1022
Bme18I GGWCC 2 cut(s) 536, 553
BmgT120I GGNCC 5 cut(s) 536, 541, 553, 918, 919
BmiI GGNNCC 5 cut(s) 290, 554, 555, 920, 994
BmrFI CCNGG 2 cut(s) 540, 1022
BmrI ACTGGG 1 cut(s) 23
BmsI GCATC 3 cut(s) 190, 367, 1025
BmuI ACTGGG 1 cut(s) 23
BpuMI CCSGG 1 cut(s) 540
BsaAI YACGTR 1 cut(s) 767
BsaBI GATNNNNATC 1 cut(s) 942
BsaJI CCNNGG 2 cut(s) 544, 1020
BsaXI ACNNNNNCTCC 2 cut(s) 292, 322
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 545, 550
Bse1I ACTGG 3 cut(s) 18, 307, 1103
Bse3DI GCAATG 2 cut(s) 978, 984
Bse8I GATNNNNATC 1 cut(s) 942
BseBI CCWGG 1 cut(s) 1022
BseDI CCNNGG 2 cut(s) 544, 1020
BseGI GGATG 5 cut(s) 460, 513, 637, 872, 1016
BseJI GATNNNNATC 1 cut(s) 942
BseLI CCNNNNNNNGG 3 cut(s) 161, 545, 550
BseMI GCAATG 2 cut(s) 978, 984
BseMII CTCAG 5 cut(s) 195, 206, 342, 399, 938
BseNI ACTGG 3 cut(s) 18, 307, 1103
BseSI GKGCMC 1 cut(s) 922
BseXI GCAGC 3 cut(s) 307, 367, 1000
Bsh1236I CGCG 1 cut(s) 897
BshFI GGCC 2 cut(s) 543, 920
BshNI GGYRCC 1 cut(s) 288
BsiHKAI GWGCWC 2 cut(s) 310, 470
BsiSI CCGG 1 cut(s) 539
BslFI GGGAC 2 cut(s) 340, 566
BslI CCNNNNNNNGG 3 cut(s) 161, 545, 550
BsmAI GTCTC 1 cut(s) 845
BsmFI GGGAC 2 cut(s) 340, 566
BsmI GAATGC 2 cut(s) 863, 1076
BsnI GGCC 2 cut(s) 543, 920
Bsp120I GGGCCC 1 cut(s) 918
Bsp1286I GDGCHC 3 cut(s) 310, 470, 922
Bsp1407I TGTACA 2 cut(s) 368, 724
Bsp143I GATC 5 cut(s) 127, 238, 349, 414, 661
BspACI CCGC 1 cut(s) 969
BspANI GGCC 2 cut(s) 543, 920
BspCNI CTCAG 5 cut(s) 196, 207, 341, 400, 939
BspFNI CGCG 1 cut(s) 897
BspLI GGNNCC 5 cut(s) 290, 554, 555, 920, 994
BspPI GGATC 2 cut(s) 233, 357
BspT107I GGYRCC 1 cut(s) 288
BsrDI GCAATG 2 cut(s) 978, 984
BsrGI TGTACA 2 cut(s) 368, 724
BsrI ACTGG 3 cut(s) 18, 307, 1103
BssECI CCNNGG 2 cut(s) 544, 1020
BssMI GATC 5 cut(s) 127, 238, 349, 414, 661
BssT1I CCWWGG 1 cut(s) 544
Bst2UI CCWGG 1 cut(s) 1022
Bst4CI ACNGT 6 cut(s) 90, 125, 404, 446, 603, 656
Bst6I CTCTTC 1 cut(s) 429
BstAUI TGTACA 2 cut(s) 368, 724
BstBAI YACGTR 1 cut(s) 767
BstC8I GCNNGC 3 cut(s) 60, 594, 991
BstDEI CTNAG 7 cut(s) 204, 215, 328, 408, 475, 815, 947
BstF5I GGATG 5 cut(s) 460, 513, 637, 872, 1016
BstFNI CGCG 1 cut(s) 897
BstHHI GCGC 1 cut(s) 882
BstKTI GATC 5 cut(s) 130, 241, 352, 417, 664
BstMAI GTCTC 1 cut(s) 845
BstMBI GATC 5 cut(s) 127, 238, 349, 414, 661
BstMWI GCNNNNNNNGC 4 cut(s) 68, 329, 990, 1022
BstNI CCWGG 1 cut(s) 1022
BstNSI RCATGY 3 cut(s) 596, 764, 886
BstSCI CCNGG 2 cut(s) 538, 1020
BstSFI CTRYAG 1 cut(s) 400
BstSLI GKGCMC 1 cut(s) 922
BstUI CGCG 1 cut(s) 897
BstV1I GCAGC 3 cut(s) 307, 367, 1000
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
BsuRI GGCC 2 cut(s) 543, 920
BtsCI GGATG 5 cut(s) 460, 513, 637, 872, 1016
BtsIMutI CAGTG 3 cut(s) 130, 300, 451
Cac8I GCNNGC 3 cut(s) 60, 594, 991
CfoI GCGC 1 cut(s) 882
Cfr13I GGNCC 5 cut(s) 536, 541, 553, 918, 919
Csp6I GTAC 4 cut(s) 298, 369, 419, 725
CviAII CATG 6 cut(s) 359, 593, 761, 877, 883, 915
CviQI GTAC 4 cut(s) 298, 369, 419, 725
DdeI CTNAG 7 cut(s) 204, 215, 328, 408, 475, 815, 947
DpnI GATC 5 cut(s) 129, 240, 351, 416, 663
DpnII GATC 5 cut(s) 127, 238, 349, 414, 661
DrdI GACNNNNNNGTC 1 cut(s) 255
DseDI GACNNNNNNGTC 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
Ecl136II GAGCTC 1 cut(s) 468
Eco130I CCWWGG 1 cut(s) 544
Eco24I GRGCYC 2 cut(s) 470, 922
Eco32I GATATC 1 cut(s) 677
Eco47I GGWCC 2 cut(s) 536, 553
Eco53kI GAGCTC 1 cut(s) 468
Eco57I CTGAAG 2 cut(s) 51, 369
Eco72I CACGTG 1 cut(s) 767
EcoICRI GAGCTC 1 cut(s) 468
EcoO109I RGGNCCY 1 cut(s) 553
EcoRI GAATTC 1 cut(s) 22
EcoRII CCWGG 1 cut(s) 1020
EcoRV GATATC 1 cut(s) 677
EcoT14I CCWWGG 1 cut(s) 544
EcoT22I ATGCAT 1 cut(s) 863
EcoT38I GRGCYC 2 cut(s) 470, 922
ErhI CCWWGG 1 cut(s) 544
FaeI CATG 6 cut(s) 362, 596, 764, 880, 886, 918
FaqI GGGAC 2 cut(s) 340, 566
FatI CATG 6 cut(s) 358, 592, 760, 876, 882, 914
FauI CCCGC 1 cut(s) 976
FbaI TGATCA 1 cut(s) 127
Fnu4HI GCNGC 3 cut(s) 321, 381, 1014
FokI GGATG 5 cut(s) 467, 520, 644, 859, 1003
FriOI GRGCYC 2 cut(s) 470, 922
Fsp4HI GCNGC 3 cut(s) 321, 381, 1014
FspAI RTGCGCAY 1 cut(s) 881
FspI TGCGCA 1 cut(s) 881
GlaI GCGC 1 cut(s) 881
GluI GCNGC 3 cut(s) 321, 381, 1014
HaeIII GGCC 2 cut(s) 543, 920
HapII CCGG 1 cut(s) 539
HhaI GCGC 1 cut(s) 882
Hin1II CATG 6 cut(s) 362, 596, 764, 880, 886, 918
Hin6I GCGC 1 cut(s) 880
HinP1I GCGC 1 cut(s) 880
HincII GTYRAC 1 cut(s) 1047
HindII GTYRAC 1 cut(s) 1047
HinfI GANTC 7 cut(s) 171, 437, 516, 636, 694, 892, 943
HpaII CCGG 1 cut(s) 539
HphI GGTGA 3 cut(s) 106, 503, 860
Hpy166II GTNNAC 3 cut(s) 300, 421, 1047
Hpy188I TCNGA 3 cut(s) 150, 216, 948
Hpy188III TCNNGA 5 cut(s) 407, 566, 659, 691, 960
Hpy8I GTNNAC 3 cut(s) 300, 421, 1047
HpyAV CCTTC 3 cut(s) 75, 543, 1003
HpyCH4III ACNGT 6 cut(s) 90, 125, 404, 446, 603, 656
HpyCH4IV ACGT 1 cut(s) 766
HpyCH4V TGCA 9 cut(s) 38, 160, 596, 764, 788, 861, 984, 1016, 1090
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 329, 990, 1022
HpyF3I CTNAG 7 cut(s) 204, 215, 328, 408, 475, 815, 947
HpySE526I ACGT 1 cut(s) 766
Hsp92II CATG 6 cut(s) 362, 596, 764, 880, 886, 918
HspAI GCGC 1 cut(s) 880
KflI GGGWCCC 1 cut(s) 553
Ksp22I TGATCA 1 cut(s) 127
Kzo9I GATC 5 cut(s) 127, 238, 349, 414, 661
LmnI GCTCC 3 cut(s) 182, 313, 998
Lsp1109I GCAGC 3 cut(s) 307, 367, 1000
LweI GCATC 3 cut(s) 190, 367, 1025
MaeII ACGT 1 cut(s) 766
MaeIII GTNAC 3 cut(s) 119, 805, 931
MalI GATC 5 cut(s) 129, 240, 351, 416, 663
MboI GATC 5 cut(s) 127, 238, 349, 414, 661
MboII GAAGA 4 cut(s) 57, 446, 554, 1092
MfeI CAATTG 1 cut(s) 615
MflI RGATCY 1 cut(s) 349
MhlI GDGCHC 3 cut(s) 310, 470, 922
MluCI AATT 7 cut(s) 22, 339, 426, 531, 615, 773, 927
MlyI GAGTC 3 cut(s) 446, 630, 688
MmeI TCCRAC 1 cut(s) 94
MnlI CCTC 5 cut(s) 110, 187, 451, 845, 999
Mph1103I ATGCAT 1 cut(s) 863
MseI TTAA 3 cut(s) 138, 335, 1001
MslI CAYNNNNRTG 3 cut(s) 765, 881, 1001
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 1 cut(s) 539
MspR9I CCNGG 2 cut(s) 540, 1022
MunI CAATTG 1 cut(s) 615
Mva1269I GAATGC 2 cut(s) 863, 1076
MvaI CCWGG 1 cut(s) 1022
MvnI CGCG 1 cut(s) 897
MwoI GCNNNNNNNGC 4 cut(s) 68, 329, 990, 1022
NciI CCSGG 1 cut(s) 540
NdeII GATC 5 cut(s) 127, 238, 349, 414, 661
NlaIII CATG 6 cut(s) 362, 596, 764, 880, 886, 918
NlaIV GGNNCC 5 cut(s) 290, 554, 555, 920, 994
NmuCI GTSAC 2 cut(s) 805, 931
NsbI TGCGCA 1 cut(s) 881
NsiI ATGCAT 1 cut(s) 863
NspI RCATGY 3 cut(s) 596, 764, 886
PaeI GCATGC 1 cut(s) 596
PctI GAATGC 2 cut(s) 863, 1076
PfeI GAWTC 4 cut(s) 171, 516, 892, 943
PkrI GCNGC 3 cut(s) 322, 382, 1015
PleI GAGTC 3 cut(s) 445, 630, 688
PmaCI CACGTG 1 cut(s) 767
PmlI CACGTG 1 cut(s) 767
PpsI GAGTC 3 cut(s) 445, 630, 688
Ppu21I YACGTR 1 cut(s) 767
PpuMI RGGWCCY 1 cut(s) 553
PsiI TTATAA 1 cut(s) 392
Psp124BI GAGCTC 1 cut(s) 470
Psp5II RGGWCCY 1 cut(s) 553
Psp6I CCWGG 1 cut(s) 1020
PspCI CACGTG 1 cut(s) 767
PspGI CCWGG 1 cut(s) 1020
PspN4I GGNNCC 5 cut(s) 290, 554, 555, 920, 994
PspOMI GGGCCC 1 cut(s) 918
PspPI GGNCC 5 cut(s) 536, 541, 553, 918, 919
PspPPI RGGWCCY 1 cut(s) 553
PsuI RGATCY 1 cut(s) 349
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 4 cut(s) 299, 370, 420, 726
RsaNI GTAC 4 cut(s) 298, 369, 419, 725
RseI CAYNNNNRTG 3 cut(s) 765, 881, 1001
SacI GAGCTC 1 cut(s) 470
SaqAI TTAA 3 cut(s) 138, 335, 1001
SatI GCNGC 3 cut(s) 321, 381, 1014
Sau3AI GATC 5 cut(s) 127, 238, 349, 414, 661
Sau96I GGNCC 5 cut(s) 536, 541, 553, 918, 919
SchI GAGTC 3 cut(s) 446, 630, 688
ScrFI CCNGG 2 cut(s) 540, 1022
SduI GDGCHC 3 cut(s) 310, 470, 922
SfaNI GCATC 3 cut(s) 190, 367, 1025
SfcI CTRYAG 1 cut(s) 400
SinI GGWCC 2 cut(s) 536, 553
SmiMI CAYNNNNRTG 3 cut(s) 765, 881, 1001
SphI GCATGC 1 cut(s) 596
Sse9I AATT 7 cut(s) 22, 339, 426, 531, 615, 773, 927
SsiI CCGC 1 cut(s) 969
SstI GAGCTC 1 cut(s) 470
StyD4I CCNGG 2 cut(s) 538, 1020
StyI CCWWGG 1 cut(s) 544
TaaI ACNGT 6 cut(s) 90, 125, 404, 446, 603, 656
TaiI ACGT 1 cut(s) 769
TaqI TCGA 1 cut(s) 519
TasI AATT 7 cut(s) 22, 339, 426, 531, 615, 773, 927
TatI WGTACW 3 cut(s) 297, 368, 724
TfiI GAWTC 4 cut(s) 171, 516, 892, 943
Tru1I TTAA 3 cut(s) 138, 335, 1001
Tru9I TTAA 3 cut(s) 138, 335, 1001
TscAI CASTG 3 cut(s) 130, 307, 451
TseFI GTSAC 2 cut(s) 805, 931
TseI GCWGC 3 cut(s) 320, 380, 1013
Tsp45I GTSAC 2 cut(s) 805, 931
TspDTI ATGAA 3 cut(s) 723, 771, 903
TspRI CASTG 3 cut(s) 130, 307, 451
VpaK11BI GGWCC 2 cut(s) 536, 553
XapI RAATTY 1 cut(s) 22
XceI RCATGY 3 cut(s) 596, 764, 886
Zsp2I ATGCAT 1 cut(s) 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.