RchiOBHm_Chr3g0483801

Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0004471
Physical Location & Seq
Reverse (-)
30130423 .. 30133368
2946 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44854

Sequence Viewer

Length: 855 bp
ATGGGAAATATAGCTGTTATTGGTGGGTGCCGTGAGTACACTGGTGCTCCATATTTTGCTGCTATCTCAGCATTAAAAATTGGTGCGGATCTGTCCCATGTTTTCTGTACAAGAGATGCTGCTTCGGTTATAAAAAGCTACAGTCCTGAGTTGATCGTACACCCAATTTTAGAAGAGTCATACAGTGTTAGGGATGAGGACAAGAGCTTTATCTTAGAAAACGTTCTTGGTGAAGTTGATAAGTGGATGGAAAGATTTGATTGTCTTGTAATTGGTCCGGGCCTTGGAAGGGACCCATTTCTTCTGGATTGTGTTAGCAAAATCATAAAGCATGCAAGACAGTCCAATGTTCCAATTGTCATAGATGGGGATGGACTCTTTCTTATTACAAACTGGCTTGATCTTGTAAGTGGATATCACTTGGCTGTCCTGACTCCAAATGTAAATGAATACAAGCGACTTGTACAAAAAGTTTTGAGTTGTGAAGTAAATGATGAAGATGCACGTGAGCAATTACTATCTCTTGCAAAAAGGATTGGTGGTGTCACTATACTTAGGAAAGGAAAAGCTGACCTCATTAGTGATGGTGAGACAGTTGAATCAGTGAGCATATATGGTTCTCCTCGAAGATGTGGTGGACAGGGAGATATACTTTTTGGAAGCGTTGCTATATTTCTTTCATGGGCCCGTCAAATTGTCACAGATGAGAATCTGAGTATCAGTTCCCGAAACCCCACAATGTTGGGTTGCATTGCTGGCTCCACTTTAATGAGGAAGGCTGCATCCCTCGCTTTTGAGAATAAGAAAAGGTCAACTCTTACTACTGACATCATAGAATGCTTGGGAAGAAGGTAA

Protein Analysis

284

Amino Acids

31.1

Weight (kDa)

5.89

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_kinase PF01256 5 - 280 2.4e-47 NAD(P)H-hydrate dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 131
AccB1I GGYRCC 1 cut(s) 27
AciI CCGC 1 cut(s) 86
AclI AACGTT 1 cut(s) 222
AclWI GGATC 1 cut(s) 96
AcvI CACGTG 1 cut(s) 506
AfaI GTAC 4 cut(s) 38, 109, 159, 465
AfiI CCNNNNNNNGG 2 cut(s) 284, 289
AgsI TTSAA 1 cut(s) 599
AjuI GAANNNNNNNTTGG 2 cut(s) 210, 242
AluBI AGCT 4 cut(s) 14, 138, 207, 569
AluI AGCT 4 cut(s) 14, 138, 207, 569
Alw21I GWGCWC 1 cut(s) 49
Alw26I GTCTC 1 cut(s) 584
AlwI GGATC 1 cut(s) 96
AoxI GGCC 2 cut(s) 280, 684
ApaI GGGCCC 1 cut(s) 688
ApeKI GCWGC 3 cut(s) 59, 119, 779
Asp700I GAANNNNTTC 1 cut(s) 222
AspS9I GGNCC 5 cut(s) 275, 280, 292, 684, 685
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 2 cut(s) 242, 599
AvaII GGWCC 2 cut(s) 275, 292
BaeGI GKGCMC 1 cut(s) 688
BanI GGYRCC 1 cut(s) 27
BanII GRGCYC 1 cut(s) 688
BbrPI CACGTG 1 cut(s) 506
Bbv12I GWGCWC 1 cut(s) 49
BbvI GCAGC 3 cut(s) 46, 106, 766
BccI CCATC 4 cut(s) 241, 359, 365, 578
BceAI ACGGC 1 cut(s) 15
BcnI CCSGG 1 cut(s) 279
BcoDI GTCTC 1 cut(s) 584
BfmI CTRYAG 1 cut(s) 139
BisI GCNGC 3 cut(s) 60, 120, 780
BlsI GCNGC 3 cut(s) 61, 121, 781
Bme1390I CCNGG 1 cut(s) 279
Bme18I GGWCC 2 cut(s) 275, 292
BmgT120I GGNCC 5 cut(s) 275, 280, 292, 684, 685
BmiI GGNNCC 5 cut(s) 29, 293, 294, 686, 760
BmrFI CCNGG 1 cut(s) 279
BmsI GCATC 3 cut(s) 106, 490, 791
BpuMI CCSGG 1 cut(s) 279
BsaAI YACGTR 1 cut(s) 506
BsaBI GATNNNNATC 1 cut(s) 708
BsaJI CCNNGG 1 cut(s) 283
BsaXI ACNNNNNCTCC 2 cut(s) 31, 61
Bsc4I CCNNNNNNNGG 2 cut(s) 284, 289
Bse1I ACTGG 2 cut(s) 46, 398
Bse3DI GCAATG 1 cut(s) 750
Bse8I GATNNNNATC 1 cut(s) 708
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 4 cut(s) 199, 252, 376, 782
BseJI GATNNNNATC 1 cut(s) 708
BseLI CCNNNNNNNGG 2 cut(s) 284, 289
BseMI GCAATG 1 cut(s) 750
BseMII CTCAG 3 cut(s) 81, 138, 704
BseNI ACTGG 2 cut(s) 46, 398
BseRI GAGGAG 1 cut(s) 612
BseSI GKGCMC 1 cut(s) 688
BseXI GCAGC 3 cut(s) 46, 106, 766
BshFI GGCC 2 cut(s) 282, 686
BshNI GGYRCC 1 cut(s) 27
BsiHKAI GWGCWC 1 cut(s) 49
BsiSI CCGG 1 cut(s) 278
BslFI GGGAC 2 cut(s) 79, 305
BslI CCNNNNNNNGG 2 cut(s) 284, 289
BsmAI GTCTC 1 cut(s) 584
BsmFI GGGAC 2 cut(s) 79, 305
BsmI GAATGC 1 cut(s) 842
BsnI GGCC 2 cut(s) 282, 686
Bsp120I GGGCCC 1 cut(s) 684
Bsp1286I GDGCHC 2 cut(s) 49, 688
Bsp1407I TGTACA 2 cut(s) 107, 463
Bsp143I GATC 3 cut(s) 88, 153, 400
BspACI CCGC 1 cut(s) 86
BspANI GGCC 2 cut(s) 282, 686
BspCNI CTCAG 3 cut(s) 80, 139, 705
BspLI GGNNCC 5 cut(s) 29, 293, 294, 686, 760
BspPI GGATC 1 cut(s) 96
BspT107I GGYRCC 1 cut(s) 27
BsrDI GCAATG 1 cut(s) 750
BsrGI TGTACA 2 cut(s) 107, 463
BsrI ACTGG 2 cut(s) 46, 398
BssECI CCNNGG 1 cut(s) 283
BssMI GATC 3 cut(s) 88, 153, 400
BssT1I CCWWGG 1 cut(s) 283
Bst4CI ACNGT 4 cut(s) 143, 185, 342, 595
Bst6I CTCTTC 1 cut(s) 168
BstAUI TGTACA 2 cut(s) 107, 463
BstBAI YACGTR 1 cut(s) 506
BstC8I GCNNGC 2 cut(s) 333, 757
BstDEI CTNAG 5 cut(s) 67, 147, 214, 554, 713
BstF5I GGATG 4 cut(s) 199, 252, 376, 782
BstKTI GATC 3 cut(s) 91, 156, 403
BstMAI GTCTC 1 cut(s) 584
BstMBI GATC 3 cut(s) 88, 153, 400
BstMWI GCNNNNNNNGC 3 cut(s) 68, 756, 788
BstNSI RCATGY 1 cut(s) 335
BstSCI CCNGG 1 cut(s) 277
BstSFI CTRYAG 1 cut(s) 139
BstSLI GKGCMC 1 cut(s) 688
BstV1I GCAGC 3 cut(s) 46, 106, 766
BstX2I RGATCY 1 cut(s) 88
BstXI CCANNNNNNTGG 1 cut(s) 742
BstYI RGATCY 1 cut(s) 88
BsuRI GGCC 2 cut(s) 282, 686
BtsCI GGATG 4 cut(s) 199, 252, 376, 782
BtsIMutI CAGTG 3 cut(s) 39, 190, 609
Cac8I GCNNGC 2 cut(s) 333, 757
Cfr13I GGNCC 5 cut(s) 275, 280, 292, 684, 685
Csp6I GTAC 4 cut(s) 37, 108, 158, 464
CviAII CATG 3 cut(s) 98, 332, 681
CviQI GTAC 4 cut(s) 37, 108, 158, 464
DdeI CTNAG 5 cut(s) 67, 147, 214, 554, 713
DpnI GATC 3 cut(s) 90, 155, 402
DpnII GATC 3 cut(s) 88, 153, 400
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
Eco130I CCWWGG 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 688
Eco32I GATATC 1 cut(s) 416
Eco47I GGWCC 2 cut(s) 275, 292
Eco72I CACGTG 1 cut(s) 506
EcoO109I RGGNCCY 1 cut(s) 292
EcoRV GATATC 1 cut(s) 416
EcoT14I CCWWGG 1 cut(s) 283
EcoT38I GRGCYC 1 cut(s) 688
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 3 cut(s) 101, 335, 684
FaqI GGGAC 2 cut(s) 79, 305
FatI CATG 3 cut(s) 97, 331, 680
Fnu4HI GCNGC 3 cut(s) 60, 120, 780
FokI GGATG 4 cut(s) 206, 259, 383, 769
FriOI GRGCYC 1 cut(s) 688
Fsp4HI GCNGC 3 cut(s) 60, 120, 780
GluI GCNGC 3 cut(s) 60, 120, 780
HaeIII GGCC 2 cut(s) 282, 686
HapII CCGG 1 cut(s) 278
Hin1II CATG 3 cut(s) 101, 335, 684
HincII GTYRAC 1 cut(s) 813
HindII GTYRAC 1 cut(s) 813
HinfI GANTC 5 cut(s) 176, 375, 433, 599, 709
HpaII CCGG 1 cut(s) 278
HphI GGTGA 2 cut(s) 242, 599
Hpy166II GTNNAC 4 cut(s) 39, 160, 638, 813
Hpy188I TCNGA 1 cut(s) 714
Hpy188III TCNNGA 4 cut(s) 146, 305, 430, 726
Hpy8I GTNNAC 4 cut(s) 39, 160, 638, 813
HpyAV CCTTC 3 cut(s) 282, 769, 843
HpyCH4III ACNGT 4 cut(s) 143, 185, 342, 595
HpyCH4IV ACGT 2 cut(s) 222, 505
HpyCH4V TGCA 5 cut(s) 335, 503, 527, 750, 782
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 756, 788
HpyF3I CTNAG 5 cut(s) 67, 147, 214, 554, 713
HpySE526I ACGT 2 cut(s) 222, 505
Hsp92II CATG 3 cut(s) 101, 335, 684
KflI GGGWCCC 1 cut(s) 292
Kzo9I GATC 3 cut(s) 88, 153, 400
LmnI GCTCC 2 cut(s) 52, 764
LpnPI CCDG 8 cut(s) 27, 159, 290, 291, 379, 443, 626, 741
Lsp1109I GCAGC 3 cut(s) 46, 106, 766
LweI GCATC 3 cut(s) 106, 490, 791
MaeII ACGT 2 cut(s) 222, 505
MaeIII GTNAC 2 cut(s) 544, 697
MalI GATC 3 cut(s) 90, 155, 402
MboI GATC 3 cut(s) 88, 153, 400
MboII GAAGA 4 cut(s) 185, 293, 509, 639
MfeI CAATTG 1 cut(s) 354
MflI RGATCY 1 cut(s) 88
MhlI GDGCHC 2 cut(s) 49, 688
MluCI AATT 6 cut(s) 78, 165, 270, 354, 512, 693
MlyI GAGTC 3 cut(s) 185, 369, 427
MnlI CCTC 5 cut(s) 190, 584, 633, 765, 797
MroXI GAANNNNTTC 1 cut(s) 222
MseI TTAA 2 cut(s) 74, 767
MslI CAYNNNNRTG 1 cut(s) 767
MspI CCGG 1 cut(s) 278
MspR9I CCNGG 1 cut(s) 279
MunI CAATTG 1 cut(s) 354
Mva1269I GAATGC 1 cut(s) 842
MwoI GCNNNNNNNGC 3 cut(s) 68, 756, 788
NciI CCSGG 1 cut(s) 279
NdeII GATC 3 cut(s) 88, 153, 400
NlaIII CATG 3 cut(s) 101, 335, 684
NlaIV GGNNCC 5 cut(s) 29, 293, 294, 686, 760
NmuCI GTSAC 2 cut(s) 544, 697
NspI RCATGY 1 cut(s) 335
PaeI GCATGC 1 cut(s) 335
PctI GAATGC 1 cut(s) 842
PdmI GAANNNNTTC 1 cut(s) 222
PfeI GAWTC 2 cut(s) 599, 709
PkrI GCNGC 3 cut(s) 61, 121, 781
PleI GAGTC 3 cut(s) 184, 369, 427
PmaCI CACGTG 1 cut(s) 506
PmlI CACGTG 1 cut(s) 506
PpsI GAGTC 3 cut(s) 184, 369, 427
Ppu21I YACGTR 1 cut(s) 506
PpuMI RGGWCCY 1 cut(s) 292
PsiI TTATAA 1 cut(s) 131
Psp1406I AACGTT 1 cut(s) 222
Psp5II RGGWCCY 1 cut(s) 292
PspCI CACGTG 1 cut(s) 506
PspN4I GGNNCC 5 cut(s) 29, 293, 294, 686, 760
PspOMI GGGCCC 1 cut(s) 684
PspPI GGNCC 5 cut(s) 275, 280, 292, 684, 685
PspPPI RGGWCCY 1 cut(s) 292
PsuI RGATCY 1 cut(s) 88
RsaI GTAC 4 cut(s) 38, 109, 159, 465
RsaNI GTAC 4 cut(s) 37, 108, 158, 464
RseI CAYNNNNRTG 1 cut(s) 767
SaqAI TTAA 2 cut(s) 74, 767
SatI GCNGC 3 cut(s) 60, 120, 780
Sau3AI GATC 3 cut(s) 88, 153, 400
Sau96I GGNCC 5 cut(s) 275, 280, 292, 684, 685
SchI GAGTC 3 cut(s) 185, 369, 427
ScrFI CCNGG 1 cut(s) 279
SduI GDGCHC 2 cut(s) 49, 688
SetI ASST 9 cut(s) 16, 140, 209, 225, 508, 571, 576, 812, 854
SfaNI GCATC 3 cut(s) 106, 490, 791
SfcI CTRYAG 1 cut(s) 139
SinI GGWCC 2 cut(s) 275, 292
SmiMI CAYNNNNRTG 1 cut(s) 767
SphI GCATGC 1 cut(s) 335
Sse9I AATT 6 cut(s) 78, 165, 270, 354, 512, 693
SsiI CCGC 1 cut(s) 86
StyD4I CCNGG 1 cut(s) 277
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 4 cut(s) 143, 185, 342, 595
TaiI ACGT 2 cut(s) 225, 508
TaqI TCGA 1 cut(s) 625
TasI AATT 6 cut(s) 78, 165, 270, 354, 512, 693
TatI WGTACW 3 cut(s) 36, 107, 463
TfiI GAWTC 2 cut(s) 599, 709
Tru1I TTAA 2 cut(s) 74, 767
Tru9I TTAA 2 cut(s) 74, 767
TscAI CASTG 3 cut(s) 46, 190, 609
TseFI GTSAC 2 cut(s) 544, 697
TseI GCWGC 3 cut(s) 59, 119, 779
Tsp45I GTSAC 2 cut(s) 544, 697
TspDTI ATGAA 3 cut(s) 462, 510, 669
TspRI CASTG 3 cut(s) 46, 190, 609
VpaK11BI GGWCC 2 cut(s) 275, 292
XceI RCATGY 1 cut(s) 335
XmnI GAANNNNTTC 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.