RLG00000023259

Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004471
Physical Location & Seq
Forward (+)
20802026 .. 20807896
5871 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023259

Sequence Viewer

Length: 867 bp
ATGGTAAACAATAAGTATTTTCTTCACTTGGTAGGTTTGATAAACGAAAGCATATCCTTTTCATCTCAGGGTGCGGATCTGTCCCATGTTTTCTGTACTAGAGATGCTGCTTCAGTTATAAAAAGCTACAGTCCTGAGTTGATCGTACACCCAATTTTAGAAGAGTCATACAGTGTCAGGGATGAGGACAAGAGCTTTATCTTAGAAGAGGTTCTTGGTGAAGTTGATAAGTGGATGGAAAGATTTGATTGTCTTGTAATTGGTCCGGGCCTGGGAAGGGACCCATTTCTTCTGGATTGTGTTAGCAAAATCATAAAGCATGCAAGACAGTCCAATGTTCCAATTGTCATAGATGGGGATGGACTCTTTCTTATTACAAACTGTCTTGATCTTGTAAGTGGATATCACTTGGCTGTCCTGACTCCAAATGTAAATGAATACAAGCGACTTGTACACAAAGTTTTGAGTTGTGAAGTAAATGATGAAGATGCACGTGAGCAATTACTATCTCTTGCAAAAAGGATTGGTGGTGTCACTATACTTAGGAAAGGAAAAGTTGACCTCATTAGTGATGGTGAGATAGTTGAATCAGTGAGCATATATGGTTCTCCTCGAAGATGTGGTGGACAGGGAGATATACTTTCTGGAAGCGTTGCTGTATTTCTTTCATGGGCCCGTCAAATTGTCACAGATGAGAATCTGAGTATCAGTTCCCGAAACCCCACAATGTTGGGTTGCATTGCTGGCTCCACTTTAATGAGGAAGGCTGCATCCCTCGCTTTTGAGAATAAGAAAAGGTCAACTCTTACTACTGACATCATAGAATGCTTGGGAAGAAGTTTGCAGGATATTTGTCCAGTTCCCTGA

Protein Analysis

289

Amino Acids

31.66

Weight (kDa)

5.45

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_kinase PF01256 21 - 276 5.2e-36 NAD(P)H-hydrate dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 119
AciI CCGC 1 cut(s) 74
AclWI GGATC 1 cut(s) 84
AcuI CTGAAG 1 cut(s) 96
AcvI CACGTG 1 cut(s) 494
AfaI GTAC 3 cut(s) 97, 147, 453
AfiI CCNNNNNNNGG 2 cut(s) 272, 277
AgsI TTSAA 1 cut(s) 587
AjnI CCWGG 1 cut(s) 270
AjuI GAANNNNNNNTTGG 2 cut(s) 198, 230
AluBI AGCT 2 cut(s) 126, 195
AluI AGCT 2 cut(s) 126, 195
AlwI GGATC 1 cut(s) 84
AoxI GGCC 2 cut(s) 268, 672
ApaI GGGCCC 1 cut(s) 676
ApeKI GCWGC 2 cut(s) 107, 767
Asp700I GAANNNNTTC 1 cut(s) 210
AspS9I GGNCC 5 cut(s) 263, 268, 280, 672, 673
AsuC2I CCSGG 1 cut(s) 267
AsuHPI GGTGA 2 cut(s) 230, 587
AvaII GGWCC 2 cut(s) 263, 280
BaeGI GKGCMC 1 cut(s) 676
BanII GRGCYC 1 cut(s) 676
BbrPI CACGTG 1 cut(s) 494
BbvI GCAGC 2 cut(s) 94, 754
BccI CCATC 4 cut(s) 229, 347, 353, 566
BciT130I CCWGG 1 cut(s) 272
BcnI CCSGG 1 cut(s) 267
BfaI CTAG 1 cut(s) 99
BfmI CTRYAG 1 cut(s) 127
BisI GCNGC 2 cut(s) 108, 768
BlsI GCNGC 2 cut(s) 109, 769
Bme1390I CCNGG 2 cut(s) 267, 272
Bme18I GGWCC 2 cut(s) 263, 280
BmgT120I GGNCC 5 cut(s) 263, 268, 280, 672, 673
BmiI GGNNCC 4 cut(s) 281, 282, 674, 748
BmrFI CCNGG 2 cut(s) 267, 272
BmsI GCATC 3 cut(s) 94, 478, 779
BpuMI CCSGG 1 cut(s) 267
BsaAI YACGTR 1 cut(s) 494
BsaBI GATNNNNATC 1 cut(s) 696
BsaJI CCNNGG 1 cut(s) 271
Bsc4I CCNNNNNNNGG 2 cut(s) 272, 277
Bse1I ACTGG 1 cut(s) 857
Bse3DI GCAATG 1 cut(s) 738
Bse8I GATNNNNATC 1 cut(s) 696
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 1 cut(s) 271
BseGI GGATG 4 cut(s) 187, 240, 364, 770
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 2 cut(s) 272, 277
BseMI GCAATG 1 cut(s) 738
BseMII CTCAG 3 cut(s) 80, 126, 692
BseNI ACTGG 1 cut(s) 857
BseRI GAGGAG 1 cut(s) 600
BseSI GKGCMC 1 cut(s) 676
BseXI GCAGC 2 cut(s) 94, 754
BshFI GGCC 2 cut(s) 270, 674
BsiSI CCGG 1 cut(s) 266
BslFI GGGAC 2 cut(s) 67, 293
BslI CCNNNNNNNGG 2 cut(s) 272, 277
BsmFI GGGAC 2 cut(s) 67, 293
BsmI GAATGC 1 cut(s) 830
BsnI GGCC 2 cut(s) 270, 674
Bsp120I GGGCCC 1 cut(s) 672
Bsp1286I GDGCHC 1 cut(s) 676
Bsp1407I TGTACA 1 cut(s) 451
Bsp143I GATC 3 cut(s) 76, 141, 388
BspACI CCGC 1 cut(s) 74
BspANI GGCC 2 cut(s) 270, 674
BspCNI CTCAG 3 cut(s) 79, 127, 693
BspLI GGNNCC 4 cut(s) 281, 282, 674, 748
BspPI GGATC 1 cut(s) 84
BsrDI GCAATG 1 cut(s) 738
BsrGI TGTACA 1 cut(s) 451
BsrI ACTGG 1 cut(s) 857
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 3 cut(s) 76, 141, 388
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 4 cut(s) 131, 173, 330, 383
Bst6I CTCTTC 2 cut(s) 156, 201
BstAUI TGTACA 1 cut(s) 451
BstBAI YACGTR 1 cut(s) 494
BstC8I GCNNGC 2 cut(s) 321, 745
BstDEI CTNAG 5 cut(s) 66, 135, 202, 542, 701
BstF5I GGATG 4 cut(s) 187, 240, 364, 770
BstKTI GATC 3 cut(s) 79, 144, 391
BstMBI GATC 3 cut(s) 76, 141, 388
BstMWI GCNNNNNNNGC 2 cut(s) 744, 776
BstNI CCWGG 1 cut(s) 272
BstNSI RCATGY 1 cut(s) 323
BstSCI CCNGG 2 cut(s) 265, 270
BstSFI CTRYAG 1 cut(s) 127
BstSLI GKGCMC 1 cut(s) 676
BstV1I GCAGC 2 cut(s) 94, 754
BstX2I RGATCY 1 cut(s) 76
BstXI CCANNNNNNTGG 1 cut(s) 730
BstYI RGATCY 1 cut(s) 76
BsuRI GGCC 2 cut(s) 270, 674
BtsCI GGATG 4 cut(s) 187, 240, 364, 770
BtsIMutI CAGTG 2 cut(s) 178, 597
Cac8I GCNNGC 2 cut(s) 321, 745
Cfr13I GGNCC 5 cut(s) 263, 268, 280, 672, 673
Csp6I GTAC 3 cut(s) 96, 146, 452
CviAII CATG 3 cut(s) 86, 320, 669
CviJI RGCY 7 cut(s) 126, 195, 270, 413, 674, 747, 767
CviKI_1 RGCY 7 cut(s) 126, 195, 270, 413, 674, 747, 767
CviQI GTAC 3 cut(s) 96, 146, 452
DdeI CTNAG 5 cut(s) 66, 135, 202, 542, 701
DpnI GATC 3 cut(s) 78, 143, 390
DpnII GATC 3 cut(s) 76, 141, 388
Eam1104I CTCTTC 2 cut(s) 156, 201
EarI CTCTTC 2 cut(s) 156, 201
Eco24I GRGCYC 1 cut(s) 676
Eco32I GATATC 1 cut(s) 404
Eco47I GGWCC 2 cut(s) 263, 280
Eco57I CTGAAG 1 cut(s) 96
Eco72I CACGTG 1 cut(s) 494
EcoO109I RGGNCCY 1 cut(s) 280
EcoRII CCWGG 1 cut(s) 270
EcoRV GATATC 1 cut(s) 404
EcoT38I GRGCYC 1 cut(s) 676
FaeI CATG 3 cut(s) 89, 323, 672
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FaqI GGGAC 2 cut(s) 67, 293
FatI CATG 3 cut(s) 85, 319, 668
Fnu4HI GCNGC 2 cut(s) 108, 768
FokI GGATG 4 cut(s) 194, 247, 371, 757
FriOI GRGCYC 1 cut(s) 676
Fsp4HI GCNGC 2 cut(s) 108, 768
FspBI CTAG 1 cut(s) 99
GluI GCNGC 2 cut(s) 108, 768
HaeIII GGCC 2 cut(s) 270, 674
HapII CCGG 1 cut(s) 266
Hin1II CATG 3 cut(s) 89, 323, 672
HincII GTYRAC 2 cut(s) 559, 801
HindII GTYRAC 2 cut(s) 559, 801
HinfI GANTC 5 cut(s) 164, 363, 421, 587, 697
HpaII CCGG 1 cut(s) 266
HphI GGTGA 2 cut(s) 230, 587
Hpy166II GTNNAC 6 cut(s) 7, 148, 454, 559, 626, 801
Hpy188I TCNGA 1 cut(s) 702
Hpy188III TCNNGA 6 cut(s) 134, 293, 386, 418, 645, 714
Hpy8I GTNNAC 6 cut(s) 7, 148, 454, 559, 626, 801
HpyAV CCTTC 2 cut(s) 270, 757
HpyCH4III ACNGT 4 cut(s) 131, 173, 330, 383
HpyCH4IV ACGT 1 cut(s) 493
HpyCH4V TGCA 6 cut(s) 323, 491, 515, 738, 770, 844
HpyF10VI GCNNNNNNNGC 2 cut(s) 744, 776
HpyF3I CTNAG 5 cut(s) 66, 135, 202, 542, 701
HpySE526I ACGT 1 cut(s) 493
Hsp92II CATG 3 cut(s) 89, 323, 672
KflI GGGWCCC 1 cut(s) 280
Kzo9I GATC 3 cut(s) 76, 141, 388
LmnI GCTCC 1 cut(s) 752
Lsp1109I GCAGC 2 cut(s) 94, 754
LweI GCATC 3 cut(s) 94, 478, 779
MaeI CTAG 1 cut(s) 99
MaeII ACGT 1 cut(s) 493
MaeIII GTNAC 2 cut(s) 532, 685
MalI GATC 3 cut(s) 78, 143, 390
MboI GATC 3 cut(s) 76, 141, 388
MboII GAAGA 7 cut(s) 14, 173, 218, 281, 497, 627, 846
MfeI CAATTG 1 cut(s) 342
MflI RGATCY 1 cut(s) 76
MhlI GDGCHC 1 cut(s) 676
MluCI AATT 5 cut(s) 153, 258, 342, 500, 681
MlyI GAGTC 3 cut(s) 173, 357, 415
MnlI CCTC 6 cut(s) 178, 202, 572, 621, 753, 785
MroXI GAANNNNTTC 1 cut(s) 210
MseI TTAA 1 cut(s) 755
MslI CAYNNNNRTG 1 cut(s) 755
MspI CCGG 1 cut(s) 266
MspR9I CCNGG 2 cut(s) 267, 272
MunI CAATTG 1 cut(s) 342
Mva1269I GAATGC 1 cut(s) 830
MvaI CCWGG 1 cut(s) 272
MwoI GCNNNNNNNGC 2 cut(s) 744, 776
NciI CCSGG 1 cut(s) 267
NdeII GATC 3 cut(s) 76, 141, 388
NlaIII CATG 3 cut(s) 89, 323, 672
NlaIV GGNNCC 4 cut(s) 281, 282, 674, 748
NmuCI GTSAC 2 cut(s) 532, 685
NspI RCATGY 1 cut(s) 323
PaeI GCATGC 1 cut(s) 323
PctI GAATGC 1 cut(s) 830
PdmI GAANNNNTTC 1 cut(s) 210
PfeI GAWTC 2 cut(s) 587, 697
PkrI GCNGC 2 cut(s) 109, 769
PleI GAGTC 3 cut(s) 172, 357, 415
PmaCI CACGTG 1 cut(s) 494
PmlI CACGTG 1 cut(s) 494
PpsI GAGTC 3 cut(s) 172, 357, 415
Ppu21I YACGTR 1 cut(s) 494
PpuMI RGGWCCY 1 cut(s) 280
PsiI TTATAA 1 cut(s) 119
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 270
PspCI CACGTG 1 cut(s) 494
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 4 cut(s) 281, 282, 674, 748
PspOMI GGGCCC 1 cut(s) 672
PspPI GGNCC 5 cut(s) 263, 268, 280, 672, 673
PspPPI RGGWCCY 1 cut(s) 280
PsuI RGATCY 1 cut(s) 76
RsaI GTAC 3 cut(s) 97, 147, 453
RsaNI GTAC 3 cut(s) 96, 146, 452
RseI CAYNNNNRTG 1 cut(s) 755
SaqAI TTAA 1 cut(s) 755
SatI GCNGC 2 cut(s) 108, 768
Sau3AI GATC 3 cut(s) 76, 141, 388
Sau96I GGNCC 5 cut(s) 263, 268, 280, 672, 673
SchI GAGTC 3 cut(s) 173, 357, 415
ScrFI CCNGG 2 cut(s) 267, 272
SduI GDGCHC 1 cut(s) 676
SetI ASST 7 cut(s) 37, 128, 197, 213, 496, 564, 800
SfaNI GCATC 3 cut(s) 94, 478, 779
SfcI CTRYAG 1 cut(s) 127
SinI GGWCC 2 cut(s) 263, 280
SmiMI CAYNNNNRTG 1 cut(s) 755
SphI GCATGC 1 cut(s) 323
Sse9I AATT 5 cut(s) 153, 258, 342, 500, 681
SsiI CCGC 1 cut(s) 74
SspMI CTAG 1 cut(s) 99
StyD4I CCNGG 2 cut(s) 265, 270
TaaI ACNGT 4 cut(s) 131, 173, 330, 383
TaiI ACGT 1 cut(s) 496
TaqI TCGA 1 cut(s) 613
TasI AATT 5 cut(s) 153, 258, 342, 500, 681
TatI WGTACW 2 cut(s) 95, 451
TfiI GAWTC 2 cut(s) 587, 697
Tru1I TTAA 1 cut(s) 755
Tru9I TTAA 1 cut(s) 755
TscAI CASTG 2 cut(s) 178, 597
TseFI GTSAC 2 cut(s) 532, 685
TseI GCWGC 2 cut(s) 107, 767
Tsp45I GTSAC 2 cut(s) 532, 685
TspDTI ATGAA 4 cut(s) 51, 450, 498, 657
TspRI CASTG 2 cut(s) 178, 597
VpaK11BI GGWCC 2 cut(s) 263, 280
XceI RCATGY 1 cut(s) 323
XmnI GAANNNNTTC 1 cut(s) 210
XspI CTAG 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.