Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007453

Physical Location & Seq

Genomic Coordinates Reverse (-)

18929355 .. 18936196

Gene Structure 6842 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1205300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2463 bp

ATGAACGGAATAAGTCCTGGGTATAAGATTCCCTCCTCCTCTTTCTATTTAATCTCACCTTCGCACCTTCTCACTCCGAGGACGAGCAGTCGCAGACCCTTTCGCAATTTTCTCTCTATTAAAAGATTGTCAACGCTTTTAGCCATGGGCAGTTCTAAAGATGGTCCAGTGAAGGACATGCCTCTTGGGGTAGATGCAACAACTGAGGAAGAATATGCATCACAGTCTAGGTTACTTCAAGACTTCACTAGTATTTCCAGCATTGACAAGGCATGGATTTTTAAATCTGATAGCGGAATTGGGTCTCAGGCAATGTTTTCAATTAGTCAACCGAATCTTTTGGCAAACAAGAGGAAGAAATTCATTCTATCCTCTCATATTTCAAGAGAAAGTAACAATTCTGTAAACTTCCAATGGTCCCCGTTCCCGGTTGAGATGACAGGAGTATCTGTATTTGTTCCATCCCCGTCCGGTGCAAAGCTTTTTGTGGTTCGGAATCCTGAGAACGAATCTCCCTGCCAATTTGAAATTTGGGGCCAAGGTCAATTGGAGAAAGAATTCCACATTCCCCAATCTGTTCATGGTTCAGTTTATGCTGATGGATGGTTTCAGGGAATTTCTTGGAACGCTGATGAAACTCTCATTGCTTATGTTGCTGAGGAACCAGCTCCCTCCAAGCCCACATTTACAGGTCAGGGATACAAGAAAAGTAGTTCCACGGATAAGGACTTTGGTAACTGGAAAGGTCAAGGGGATTGGAAGGAAGAATGGGGGGAAACCTATGCTGGAAAAAGGCAGCCTGCACTTTTTGTCATCAATATTAACAGCGGAGAGGCACAAGCTGTCAAAGGAATTGAAAAGTCTTTGAGTGTTGGTCAAGTTGTATGGGCGCCACCAGTTAAAGGCTCGCATCAATCGTTGGTTTTTGTTGGGTGGTCAGAAGGTATCAGAAAGCTTGGTATCAAGTACTGCTTTAACAGGCCCTGTGCATTGTATGTGGTCAAGGCACCAAATTTTGAATCAGAAGCAAATGGATCTGAACTCAAAGCTAGTTCAACTGAAGATCTTCCTGCTGTAAAGCTGACTCAAAGCATAAGTAGTGCTTTCTATCCACGGTTCTGCCCAGATGGAAAATTCCTTTTGTTTTTATCGGCTAGAAGTTCTGTGGATTCCGGGGCACATTCTGCAACAGATTCTCTTCACAGAATAGACTGGCCAGTGGATGGAGTGCTATCCTCATCTGCAAAAATTGTTGATGTGATTCCTGTTGTTATGTGTGCTGAGGATGGTTGCTTCCCTGGGCTTTACTGGTCAAGTATCCTTAGTAATCCATGGCTTTCTGATGGATGCACAATGATCATATCTTCTATCTGGGGTAGCTGTCAAGTGATACTTTCTGTGAATGTTTTGAGCGGGGAAGTATCACGTATCAGCTCTGCTGATTCAAATTCATGGGATGTTCTTACACTGGATGGGGACAATATCATTGCTGTGTCTAGCAGTCCAGTAGATGTACCTCATATCAATTATGGTTACCTTGTTGATAAAGAAAGTACAAGTACCGCATGGAGTTGGTTAAATGTATCAAGCCCCCCAAGTGAATGCTCTGAGAAGGTTAAATCTTTGCTCTCCTCTCTGCAATTCAGTATACTGAAGGTTCCTGTCAGGGATGTTTCTGGTAGCGTAACAAAAGGTGCTGCCAAACCAATTGAAGCCATATTTGTGTCTTCCAAAACTAAAAGAAATGATTCATTTGACCCATTAATTGTAATCCTCCACGGAGGCCCGCACTCTGTTTCATTGTCAAGCTATTCAAAGTCCTTAGCATTTCTCTCTTCAATTGGGTTCAGTTTGTTACTTGTAAATTATAGAGGTTCGCTGGGTTTTGGTGAAGAAGCACTTCAGTCCCTTCTAGGGAAAATAGGGTCCCAGGACGTGGATGATGTGCTGGTGGCTATCGATCACGTCATTGACAAGGGGCTTGCCAGTGCATCAAAAATTGCAGTTCTTGGTGGTTCTCACGGCGGCTTTCTGACTACTCACTTGATTGGCCAGGCACCAGATAAGTTTGTTGTGGCAGCAGTGAGGAACCCCGCCTGTAATCTCGCATTAATGATCGGTACGACAGACATCCCAGATTGGTGCTATGTGGAGGCCTATGGAAGTGAGGGTAGAAATAACTACACAGAAGCACCTTCTGCTGAGCATCTGACTCTCTTGCACAGCAAATCTCCTATTTCACACGTCTCAAAGGTCAAAACACCCACCCTCTTTCTTTTGGGTGCTCAGGATCTTCGTGTTCCAATTTCTACTGGACTTCAATATGCTCGGGCGCTTAAGGAAAAAGGAGTTCCTGTCAAAGTCATTGTGTTTCCACAGGATACTCATGGAATTGAGAGGCCACAATCGGACTTTGAGAGCTTTCTTAACATTGGTGTCTGGTTCAAGAAGTATTTACCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004177 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016787 GO:0017171 GO:0019538 GO:0022607 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0051259 GO:0051260 GO:0051262 GO:0051289 GO:0065003 GO:0070008 GO:0070009 GO:0070011 GO:0071704 GO:0071840 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PD40 Peptidase_S9

Protein Analysis

821

Amino Acids

88.95

Weight (kDa)

6.4

Isoelectric Point (pI)

48.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
APEH_N	PF19283	58 - 540	7.4e-259	Acylamino-acid-releasing enzyme, N-terminal domain
BD-FAE	PF20434	584 - 773	5.1e-06	BD-FAE
Hydrolase_4	PF12146	587 - 706	8.5e-06	Serine aminopeptidase, S33
Peptidase_S9	PF00326	603 - 819	4.4e-36	Prolyl oligopeptidase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007453)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G14570
fragaria_vesca	FvH4_4g02060 FvH4_4g02162
malus_domestica	MD13G1203600.v1.1 MD16G1205300.v1.1 MD16G1205600.v1.1
prunus_persica	Prupe.1G026800_v2.0.a1
pyrus_communis	pycom13g17690 pycom16g17300 pycom16g17310
rosa_chinensis	RchiOBHm_Chr4g0389431 RchiOBHm_Chr4g0389461
rosa_laevigata	RLG00000009980 RLG00000009982
rosa_multiflora	Rmu_sc0002498.1_g000017
rosa_roxburghii	Rroxscaffold_5G00335440 Rroxscaffold_5G00335460
rosa_rugosa	Rorug03G0323200
rosa_samantha	Rh4AG027700 Rh4AG027800 Rh4BG020600 Rh4BG021000 Rh4DG021300 Rh4DG021400
rosa_wichuraiana	Rw4G002010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	889, 1006, 2056
AccB7I	CCANNNNNTGG	2 cut(s)	1223, 1936
AccBSI	CCGCTC	1 cut(s)	1413
AccI	GTMKAC	1 cut(s)	1648
AciI	CCGC	7 cut(s)	294, 828, 1413, 1563, 1787, 2025, 2094
AclWI	GGATC	2 cut(s)	1042, 2298
AcoI	YGGCCR	2 cut(s)	1214, 2050
AcsI	RAATTY	7 cut(s)	359, 528, 557, 615, 1012, 1133, 1447
AcuI	CTGAAG	3 cut(s)	1080, 1673, 1886
AcyI	GRCGYC	1 cut(s)	890
AfaI	GTAC	5 cut(s)	968, 1515, 1555, 1561, 2122
AfiI	CCNNNNNNNGG	5 cut(s)	427, 902, 1223, 1914, 1936
AflII	CTTAAG	1 cut(s)	2336
AflIII	ACRYGT	1 cut(s)	2242
AhdI	GACNNNNNGTC	1 cut(s)	87
AhlI	ACTAGT	1 cut(s)	248
AjiI	CACGTC	3 cut(s)	1936, 1966, 2245
AjnI	CCWGG	4 cut(s)	16, 1297, 1929, 2052
Alw21I	GWGCWC	1 cut(s)	2287
Alw26I	GTCTC	2 cut(s)	309, 2251
AlwI	GGATC	2 cut(s)	1042, 2298
AlwNI	CAGNNNCTG	1 cut(s)	984
Ama87I	CYCGRG	1 cut(s)	2328
AoxI	GGCC	7 cut(s)	535, 980, 1214, 1783, 2050, 2154, 2399
ApeKI	GCWGC	3 cut(s)	796, 1697, 2078
ApoI	RAATTY	7 cut(s)	359, 528, 557, 615, 1012, 1133, 1447
AseI	ATTAAT	2 cut(s)	1763, 2111
Asp700I	GAANNNNTTC	5 cut(s)	359, 557, 1065, 1747, 1899
AspLEI	GCGC	2 cut(s)	892, 2335
AspS9I	GGNCC	6 cut(s)	164, 417, 535, 981, 1784, 1926
AsuC2I	CCSGG	2 cut(s)	428, 1174
AsuHPI	GGTGA	2 cut(s)	48, 1901
AvaI	CYCGRG	1 cut(s)	2328
AvaII	GGWCC	3 cut(s)	164, 417, 1926
BaeGI	GKGCMC	1 cut(s)	1180
BaeI	ACNNNNGTAYC	2 cut(s)	429, 462
BalI	TGGCCA	2 cut(s)	1216, 2052
BanI	GGYRCC	3 cut(s)	889, 1006, 2056
BbsI	GAAGAC	1 cut(s)	1719
Bbv12I	GWGCWC	1 cut(s)	2287
BbvCI	CCTCAGC	2 cut(s)	657, 1281
BbvI	GCAGC	3 cut(s)	808, 1684, 2090
BccI	CCATC	9 cut(s)	155, 469, 593, 597, 1121, 1217, 1280, 1337, 1466
BceAI	ACGGC	1 cut(s)	2038
BciT130I	CCWGG	4 cut(s)	18, 1299, 1931, 2054
BciVI	GTATCC	3 cut(s)	692, 1328, 2374
BclI	TGATCA	1 cut(s)	1356
BcnI	CCSGG	2 cut(s)	428, 1174
BcoDI	GTCTC	2 cut(s)	309, 2251
BcuI	ACTAGT	1 cut(s)	248
BfaI	CTAG	6 cut(s)	228, 249, 1050, 1155, 1497, 1913
BfoI	RGCGCY	2 cut(s)	893, 2336
BfrI	CTTAAG	1 cut(s)	2336
BfuI	GTATCC	3 cut(s)	692, 1328, 2374
BglII	AGATCT	1 cut(s)	1063
BisI	GCNGC	4 cut(s)	797, 1698, 2026, 2079
BlpI	GCTNAGC	1 cut(s)	2202
BlsI	GCNGC	4 cut(s)	798, 1699, 2027, 2080
BmcAI	AGTACT	1 cut(s)	968
Bme1390I	CCNGG	6 cut(s)	18, 428, 1174, 1299, 1931, 2054
Bme18I	GGWCC	3 cut(s)	164, 417, 1926
BmeRI	GACNNNNNGTC	1 cut(s)	87
BmeT110I	CYCGRG	1 cut(s)	2328
BmgBI	CACGTC	3 cut(s)	1936, 1966, 2245
BmgT120I	GGNCC	6 cut(s)	164, 417, 535, 981, 1784, 1926
BmrFI	CCNGG	6 cut(s)	18, 428, 1174, 1299, 1931, 2054
BmsI	GCATC	6 cut(s)	184, 227, 919, 1337, 2000, 2215
BpiI	GAAGAC	1 cut(s)	1719
Bpu10I	CCTNAGC	4 cut(s)	657, 1281, 1822, 2286
Bpu1102I	GCTNAGC	1 cut(s)	2202
BpuMI	CCSGG	2 cut(s)	428, 1174
Bsa29I	ATCGAT	1 cut(s)	1959
BsaAI	YACGTR	1 cut(s)	1427
BsaHI	GRCGYC	1 cut(s)	890
BsaI	GGTCTC	1 cut(s)	309
BsaWI	WCCGGW	1 cut(s)	470
BsaXI	ACNNNNNCTCC	4 cut(s)	435, 465, 2340, 2370
Bsc4I	CCNNNNNNNGG	5 cut(s)	427, 902, 1223, 1914, 1936
Bse3DI	GCAATG	3 cut(s)	318, 642, 1485
BseBI	CCWGG	4 cut(s)	18, 1299, 1931, 2054
BseCI	ATCGAT	1 cut(s)	1959
BseLI	CCNNNNNNNGG	5 cut(s)	427, 902, 1223, 1914, 1936
BseMI	GCAATG	3 cut(s)	318, 642, 1485
BseMII	CTCAG	8 cut(s)	195, 320, 492, 648, 1272, 1599, 2193, 2300
BseRI	GAGGAG	3 cut(s)	25, 28, 1621
BseSI	GKGCMC	1 cut(s)	1180
BseXI	GCAGC	3 cut(s)	808, 1684, 2090
BseYI	CCCAGC	1 cut(s)	1879
BsgI	GTGCAG	1 cut(s)	786
BshFI	GGCC	7 cut(s)	537, 982, 1216, 1785, 2052, 2156, 2401
BshNI	GGYRCC	3 cut(s)	889, 1006, 2056
BshVI	ATCGAT	1 cut(s)	1959
BsiHKAI	GWGCWC	1 cut(s)	2287
BsiHKCI	CYCGRG	1 cut(s)	2328
BsiSI	CCGG	3 cut(s)	428, 471, 1173
BslFI	GGGAC	4 cut(s)	403, 1490, 1891, 1912
BslI	CCNNNNNNNGG	5 cut(s)	427, 902, 1223, 1914, 1936
BsmAI	GTCTC	2 cut(s)	309, 2251
BsmBI	CGTCTC	1 cut(s)	2251
BsmFI	GGGAC	4 cut(s)	403, 1490, 1891, 1912
BsmI	GAATGC	1 cut(s)	1607
BsnI	GGCC	7 cut(s)	537, 982, 1216, 1785, 2052, 2156, 2401
Bso31I	GGTCTC	1 cut(s)	309
BsoBI	CYCGRG	1 cut(s)	2328
Bsp1286I	GDGCHC	2 cut(s)	1180, 2287
Bsp143I	GATC	6 cut(s)	1034, 1063, 1356, 1960, 2115, 2290
Bsp1720I	GCTNAGC	1 cut(s)	2202
Bsp19I	CCATGG	2 cut(s)	144, 1331
BspACI	CCGC	7 cut(s)	294, 828, 1413, 1563, 1787, 2025, 2094
BspANI	GGCC	7 cut(s)	537, 982, 1216, 1785, 2052, 2156, 2401
BspCNI	CTCAG	8 cut(s)	196, 319, 493, 649, 1273, 1600, 2194, 2299
BspDI	ATCGAT	1 cut(s)	1959
BspPI	GGATC	2 cut(s)	1042, 2298
BspT107I	GGYRCC	3 cut(s)	889, 1006, 2056
BspTI	CTTAAG	1 cut(s)	2336
BspTNI	GGTCTC	1 cut(s)	309
BsrBI	CCGCTC	1 cut(s)	1413
BsrDI	GCAATG	3 cut(s)	318, 642, 1485
BssMI	GATC	6 cut(s)	1034, 1063, 1356, 1960, 2115, 2290
BssNAI	GTATAC	1 cut(s)	1649
BssNI	GRCGYC	1 cut(s)	890
BssT1I	CCWWGG	3 cut(s)	144, 538, 1331
Bst1107I	GTATAC	1 cut(s)	1649
Bst2UI	CCWGG	4 cut(s)	18, 1299, 1931, 2054
Bst4CI	ACNGT	3 cut(s)	225, 1116, 2460
Bst6I	CTCTTC	2 cut(s)	1203, 1840
BstACI	GRCGYC	1 cut(s)	890
BstAFI	CTTAAG	1 cut(s)	2336
BstAPI	GCANNNNNTGC	2 cut(s)	1184, 2198
BstBAI	YACGTR	1 cut(s)	1427
BstC8I	GCNNGC	4 cut(s)	801, 908, 1787, 1983
BstDSI	CCRYGG	5 cut(s)	144, 717, 1112, 1331, 1777
BstEII	GGTNACC	1 cut(s)	1532
BstH2I	RGCGCY	2 cut(s)	893, 2336
BstHHI	GCGC	2 cut(s)	892, 2335
BstKTI	GATC	6 cut(s)	1037, 1066, 1359, 1963, 2118, 2293
BstMAI	GTCTC	2 cut(s)	309, 2251
BstMBI	GATC	6 cut(s)	1034, 1063, 1356, 1960, 2115, 2290
BstMWI	GCNNNNNNNGC	3 cut(s)	653, 1184, 2198
BstNI	CCWGG	4 cut(s)	18, 1299, 1931, 2054
BstNSI	RCATGY	1 cut(s)	181
BstPI	GGTNACC	1 cut(s)	1532
BstSCI	CCNGG	6 cut(s)	16, 426, 1172, 1297, 1929, 2052
BstSLI	GKGCMC	1 cut(s)	1180
BstV1I	GCAGC	3 cut(s)	808, 1684, 2090
BstV2I	GAAGAC	1 cut(s)	1719
BstX2I	RGATCY	3 cut(s)	1034, 1063, 2290
BstYI	RGATCY	3 cut(s)	1034, 1063, 2290
BstZ17I	GTATAC	1 cut(s)	1649
Bsu15I	ATCGAT	1 cut(s)	1959
BsuI	GTATCC	3 cut(s)	692, 1328, 2374
BsuRI	GGCC	7 cut(s)	537, 982, 1216, 1785, 2052, 2156, 2401
BsuTUI	ATCGAT	1 cut(s)	1959
BtgI	CCRYGG	5 cut(s)	144, 717, 1112, 1331, 1777
BtrI	CACGTC	3 cut(s)	1936, 1966, 2245
BtsI	GCAGTG	1 cut(s)	2088
BtsIMutI	CAGTG	5 cut(s)	174, 1224, 1466, 1993, 2088
Cac8I	GCNNGC	4 cut(s)	801, 908, 1787, 1983
CaiI	CAGNNNCTG	1 cut(s)	984
CfoI	GCGC	2 cut(s)	892, 2335
Cfr13I	GGNCC	6 cut(s)	164, 417, 535, 981, 1784, 1926
ClaI	ATCGAT	1 cut(s)	1959
Csp6I	GTAC	5 cut(s)	967, 1514, 1554, 1560, 2121
CviAII	CATG	8 cut(s)	145, 178, 273, 581, 1332, 1452, 1566, 2387
CviQI	GTAC	5 cut(s)	967, 1514, 1554, 1560, 2121
DinI	GGCGCC	1 cut(s)	891
DpnI	GATC	6 cut(s)	1036, 1065, 1358, 1962, 2117, 2292
DpnII	GATC	6 cut(s)	1034, 1063, 1356, 1960, 2115, 2290
DraI	TTTAAA	1 cut(s)	283
DriI	GACNNNNNGTC	1 cut(s)	87
EaeI	YGGCCR	2 cut(s)	1214, 2050
Eam1104I	CTCTTC	2 cut(s)	1203, 1840
Eam1105I	GACNNNNNGTC	1 cut(s)	87
EarI	CTCTTC	2 cut(s)	1203, 1840
Eco130I	CCWWGG	3 cut(s)	144, 538, 1331
Eco147I	AGGCCT	1 cut(s)	2156
Eco31I	GGTCTC	1 cut(s)	309
Eco47I	GGWCC	3 cut(s)	164, 417, 1926
Eco57I	CTGAAG	3 cut(s)	1080, 1673, 1886
Eco88I	CYCGRG	1 cut(s)	2328
Eco91I	GGTNACC	1 cut(s)	1532
EcoO109I	RGGNCCY	2 cut(s)	981, 1926
EcoO65I	GGTNACC	1 cut(s)	1532
EcoRI	GAATTC	1 cut(s)	557
EcoRII	CCWGG	4 cut(s)	16, 1297, 1929, 2052
EcoT14I	CCWWGG	3 cut(s)	144, 538, 1331
EcoT22I	ATGCAT	1 cut(s)	220
EgeI	GGCGCC	1 cut(s)	891
EheI	GGCGCC	1 cut(s)	891
ErhI	CCWWGG	3 cut(s)	144, 538, 1331
Esp3I	CGTCTC	1 cut(s)	2251
FaeI	CATG	8 cut(s)	148, 181, 276, 584, 1335, 1455, 1569, 2390
FalI	AAGNNNNNCTT	8 cut(s)	956, 988, 1087, 1119, 1377, 1409, 1884, 1916
FaqI	GGGAC	4 cut(s)	403, 1490, 1891, 1912
FatI	CATG	8 cut(s)	144, 177, 272, 580, 1331, 1451, 1565, 2386
FauI	CCCGC	3 cut(s)	1406, 1794, 2101
FbaI	TGATCA	1 cut(s)	1356
FblI	GTMKAC	1 cut(s)	1648
Fnu4HI	GCNGC	4 cut(s)	797, 1698, 2026, 2079
Fsp4HI	GCNGC	4 cut(s)	797, 1698, 2026, 2079
FspBI	CTAG	6 cut(s)	228, 249, 1050, 1155, 1497, 1913
GlaI	GCGC	2 cut(s)	891, 2334
GluI	GCNGC	4 cut(s)	797, 1698, 2026, 2079
GsaI	CCCAGC	1 cut(s)	1883
HaeII	RGCGCY	2 cut(s)	893, 2336
HaeIII	GGCC	7 cut(s)	537, 982, 1216, 1785, 2052, 2156, 2401
HapII	CCGG	3 cut(s)	428, 471, 1173
HhaI	GCGC	2 cut(s)	892, 2335
Hin1I	GRCGYC	1 cut(s)	890
Hin1II	CATG	8 cut(s)	148, 181, 276, 584, 1335, 1455, 1569, 2390
Hin6I	GCGC	2 cut(s)	890, 2333
HinP1I	GCGC	2 cut(s)	890, 2333
HincII	GTYRAC	2 cut(s)	132, 329
HindII	GTYRAC	2 cut(s)	132, 329
HindIII	AAGCTT	2 cut(s)	479, 953
HpaII	CCGG	3 cut(s)	428, 471, 1173
HphI	GGTGA	2 cut(s)	48, 1901
Hpy166II	GTNNAC	4 cut(s)	132, 329, 406, 1649
Hpy188III	TCNNGA	5 cut(s)	239, 384, 500, 2288, 2446
Hpy8I	GTNNAC	4 cut(s)	132, 329, 406, 1649
HpyAV	CCTTC	9 cut(s)	69, 77, 166, 754, 935, 1606, 1648, 1919, 2205
HpyCH4III	ACNGT	3 cut(s)	225, 1116, 2460
HpyCH4IV	ACGT	4 cut(s)	1426, 1935, 1965, 2244
HpyF10VI	GCNNNNNNNGC	3 cut(s)	653, 1184, 2198
HpySE526I	ACGT	4 cut(s)	1426, 1935, 1965, 2244
Hsp92I	GRCGYC	1 cut(s)	890
Hsp92II	CATG	8 cut(s)	148, 181, 276, 584, 1335, 1455, 1569, 2390
HspAI	GCGC	2 cut(s)	890, 2333
KasI	GGCGCC	1 cut(s)	889
KflI	GGGWCCC	1 cut(s)	1926
Ksp22I	TGATCA	1 cut(s)	1356
Kzo9I	GATC	6 cut(s)	1034, 1063, 1356, 1960, 2115, 2290
LmnI	GCTCC	1 cut(s)	673
Lsp1109I	GCAGC	3 cut(s)	808, 1684, 2090
LweI	GCATC	6 cut(s)	184, 227, 919, 1337, 2000, 2215
MaeI	CTAG	6 cut(s)	228, 249, 1050, 1155, 1497, 1913
MaeII	ACGT	4 cut(s)	1426, 1935, 1965, 2244
MaeIII	GTNAC	6 cut(s)	231, 392, 734, 1532, 1684, 1854
MalI	GATC	6 cut(s)	1036, 1065, 1358, 1962, 2117, 2292
MbiI	CCGCTC	1 cut(s)	1413
MboI	GATC	6 cut(s)	1034, 1063, 1356, 1960, 2115, 2290
MfeI	CAATTG	3 cut(s)	545, 1707, 1839
MflI	RGATCY	3 cut(s)	1034, 1063, 2290
MhlI	GDGCHC	2 cut(s)	1180, 2287
MlsI	TGGCCA	2 cut(s)	1216, 2052
MluNI	TGGCCA	2 cut(s)	1216, 2052
Mly113I	GGCGCC	1 cut(s)	890
MlyI	GAGTC	2 cut(s)	1078, 2206
Mox20I	TGGCCA	2 cut(s)	1216, 2052
Mph1103I	ATGCAT	1 cut(s)	220
MroXI	GAANNNNTTC	5 cut(s)	359, 557, 1065, 1747, 1899
MscI	TGGCCA	2 cut(s)	1216, 2052
MslI	CAYNNNNRTG	2 cut(s)	1490, 1721
Msp20I	TGGCCA	2 cut(s)	1216, 2052
MspA1I	CMGCKG	1 cut(s)	828
MspCI	CTTAAG	1 cut(s)	2336
MspI	CCGG	3 cut(s)	428, 471, 1173
MspR9I	CCNGG	6 cut(s)	18, 428, 1174, 1299, 1931, 2054
MunI	CAATTG	3 cut(s)	545, 1707, 1839
Mva1269I	GAATGC	1 cut(s)	1607
MvaI	CCWGG	4 cut(s)	18, 1299, 1931, 2054
MwoI	GCNNNNNNNGC	3 cut(s)	653, 1184, 2198
NarI	GGCGCC	1 cut(s)	890
NciI	CCSGG	2 cut(s)	428, 1174
NcoI	CCATGG	2 cut(s)	144, 1331
NdeII	GATC	6 cut(s)	1034, 1063, 1356, 1960, 2115, 2290
NlaIII	CATG	8 cut(s)	148, 181, 276, 584, 1335, 1455, 1569, 2390
NsiI	ATGCAT	1 cut(s)	220
NspI	RCATGY	1 cut(s)	181
PasI	CCCWGGG	1 cut(s)	1298
PceI	AGGCCT	1 cut(s)	2156
PcsI	WCGNNNNNNNCGW	1 cut(s)	914
PctI	GAATGC	1 cut(s)	1607
PdmI	GAANNNNTTC	5 cut(s)	359, 557, 1065, 1747, 1899
PflMI	CCANNNNNTGG	2 cut(s)	1223, 1936
PkrI	GCNGC	4 cut(s)	798, 1699, 2027, 2080
PleI	GAGTC	2 cut(s)	1078, 2206
PluTI	GGCGCC	1 cut(s)	893
PpsI	GAGTC	2 cut(s)	1078, 2206
Ppu21I	YACGTR	1 cut(s)	1427
PpuMI	RGGWCCY	1 cut(s)	1926
PshBI	ATTAAT	2 cut(s)	1763, 2111
Psp5II	RGGWCCY	1 cut(s)	1926
Psp6I	CCWGG	4 cut(s)	16, 1297, 1929, 2052
PspEI	GGTNACC	1 cut(s)	1532
PspFI	CCCAGC	1 cut(s)	1879
PspGI	CCWGG	4 cut(s)	16, 1297, 1929, 2052
PspPI	GGNCC	6 cut(s)	164, 417, 535, 981, 1784, 1926
PspPPI	RGGWCCY	1 cut(s)	1926
PstNI	CAGNNNCTG	1 cut(s)	984
PsuI	RGATCY	3 cut(s)	1034, 1063, 2290
RsaI	GTAC	5 cut(s)	968, 1515, 1555, 1561, 2122
RsaNI	GTAC	5 cut(s)	967, 1514, 1554, 1560, 2121
RseI	CAYNNNNRTG	2 cut(s)	1490, 1721
SatI	GCNGC	4 cut(s)	797, 1698, 2026, 2079
Sau3AI	GATC	6 cut(s)	1034, 1063, 1356, 1960, 2115, 2290
Sau96I	GGNCC	6 cut(s)	164, 417, 535, 981, 1784, 1926
ScaI	AGTACT	1 cut(s)	968
SchI	GAGTC	2 cut(s)	1078, 2206
ScrFI	CCNGG	6 cut(s)	18, 428, 1174, 1299, 1931, 2054
SduI	GDGCHC	2 cut(s)	1180, 2287
SfaNI	GCATC	6 cut(s)	184, 227, 919, 1337, 2000, 2215
SfoI	GGCGCC	1 cut(s)	891
SinI	GGWCC	3 cut(s)	164, 417, 1926
SmiMI	CAYNNNNRTG	2 cut(s)	1490, 1721
SmlI	CTYRAG	1 cut(s)	2336
SmoI	CTYRAG	1 cut(s)	2336
SpeI	ACTAGT	1 cut(s)	248
SseBI	AGGCCT	1 cut(s)	2156
SsiI	CCGC	7 cut(s)	294, 828, 1413, 1563, 1787, 2025, 2094
SspDI	GGCGCC	1 cut(s)	889
SspI	AATATT	1 cut(s)	820
SspMI	CTAG	6 cut(s)	228, 249, 1050, 1155, 1497, 1913
StuI	AGGCCT	1 cut(s)	2156
StyD4I	CCNGG	6 cut(s)	16, 426, 1172, 1297, 1929, 2052
StyI	CCWWGG	3 cut(s)	144, 538, 1331
TaaI	ACNGT	3 cut(s)	225, 1116, 2460
TaiI	ACGT	4 cut(s)	1429, 1938, 1968, 2247
TaqI	TCGA	1 cut(s)	1959
TatI	WGTACW	2 cut(s)	966, 1553
TauI	GCSGC	1 cut(s)	2028
TscAI	CASTG	5 cut(s)	174, 1224, 1473, 1993, 2088
TseI	GCWGC	3 cut(s)	796, 1697, 2078
TspDTI	ATGAA	7 cut(s)	17, 352, 569, 648, 1440, 1740, 1788
TspGWI	ACGGA	3 cut(s)	21, 734, 1794
TspRI	CASTG	5 cut(s)	174, 1224, 1473, 1993, 2088
Van91I	CCANNNNNTGG	2 cut(s)	1223, 1936
Vha464I	CTTAAG	1 cut(s)	2336
VpaK11BI	GGWCC	3 cut(s)	164, 417, 1926
VspI	ATTAAT	2 cut(s)	1763, 2111
XapI	RAATTY	7 cut(s)	359, 528, 557, 615, 1012, 1133, 1447
XceI	RCATGY	1 cut(s)	181
XcmI	CCANNNNNNNNNTGG	1 cut(s)	578
XmiI	GTMKAC	1 cut(s)	1648
XmnI	GAANNNNTTC	5 cut(s)	359, 557, 1065, 1747, 1899
XspI	CTAG	6 cut(s)	228, 249, 1050, 1155, 1497, 1913
ZrmI	AGTACT	1 cut(s)	968
Zsp2I	ATGCAT	1 cut(s)	220

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.