Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007453

Physical Location & Seq

Genomic Coordinates Reverse (-)

18974439 .. 18979426

Gene Structure 4988 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1205600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1854 bp

ATGGGAATTTCTTGGAACGCTGATGAAACTCTCGTTGCTTATGTTGCTGAGGAACCAGCTCCCTCCAAGCCCACATTTACAGGTCAGGGCTACAAGAAAAGTAGTTCCACGGATAAGGACTTTGGTAACTGGAAAGGTCAAGGGGATTGGAAGGAAGAATGGGGGGAAACCTATGCTGGAAAAAGGCAGCCTGCACTTTTTGTCATCAATATTAACAGCGGAGAGGCACAAGCTGTCAAAGGAATTGAAAAGTCTTTGAGTGTTGGTCAAGTTGTATGGGCGCCACCAGTTCAAGACTCGCATCAATCTTTGGTTTTTGTTGGGTGGTCAGAAGGTATCAGAAAGCTTGGTATCAAGTACTGCTTTAACAGGCCCTGTGCATTGTATGTGGTCAAGGCACCAAATTTTGAATCAGAAGCAAATGGATCTGAACTCAAAGCTAATTCAACTGAAGATCTTCCTGCTGTAAAGCTGACTCAAAGCATAAGTAGTGCTTTCTATCCACGGTTCTGCCCAGATGGAAAATTCCTTTTGTTTTTATCGGCTAGAAGTTCTGTGGATTCTGGGGCACATTCTGCAACAGATTCTCTTCACAGAATAGACTGGCCAGTGGATGGAGTGCTATCCTCATCTGCAAAAATTGTTGATGTGATTCCTGTTGTTATGTGTGCTGAGGATGGTTGCTTCCCTGGGCTTTACTGGTCAAGTATCCTTAGTAATCCATGGCTTTCTGATGGATGCACAATGATCATATCTTCTATCTGGGGTAGCTGTCAAGTGATACTTTCTGTGAATGTTTTGAGTGGGGAAGTATCACGTATCAGCTCTGCTGATTCAAATTCATGGGATGTTCTTACACTGGATGGGGACAATATCATTGCTGTGTCTAGCAGTCCAGTAGATGTACCTCATATCAATTATGGTTACCTTGTTGATAAAGAAAGTACAAGTACCGCATGGAGTTGGTTAAATGTATCAAGCCCCCCAAGTGAATGCTCTGAGAAGGTTAAATCTTTGCTCTCCTCTCTGCAATTCAGTATACTGAAGGTTCCTGTCAGGGATGTCTCTGGTAGCGTAACAAAAGGTGCTGCCAAACCAATTGAAGCCATATTTGTGTCTTCCAAAACTAAAAGAAATGATTCGTTTGACCCATTAATTGTAATCCTCCATGGAGGCCCGCACTTTGTTTCATTGTCAAGCTATTCAAAGTCCTTAGCATTTCTCTCGTCAATTGGGTTCAGTTTGTTACTTGTAAATTATAGAGGTTCGCTGGGTTTTGGTGAAGAAGCACTTCAGTCCCTTCTAGGGAAAATAGGGTCCCAGGACGTGGATGATGTGCTGGTGGCTATCGATCACGTCATTGACAAGGGGCTTGCCAGTGCATCAAAAATTGCAGTTCTTGGTGGTTCTCATGGCGGCTTTCTGACAACTCACTTGATTGGCCAGGCACCAGATAAGTTTGTTGTGGCAGCAACGAGGAACCCCGTCTGTAATCTCGCATTAATGATCGGTACGACAGACATCCCAGATTGGTGCTATGTGGAGGCCTATGGAAGTGAGGGTAGAAATAACTACACAGAAGCACCTTCTGCTGAGCATCTGACTCTCTTGCACAGCAAATCTCCTATTTCACACGTCTCAAAGGTCAAAACACCCATCCTTTTTCTTTTGGGTGCTCAGGATCTTCGTGTTCCAGTTTCTACTGGATTTCAATATGCTCGGGCGCTTAAGGAAAAAGGAGTTCCTGTCAAAGTCATTGTGTTTCCACAGGATACTCATGGAATTGAGAGGCCACAATCGGACTTTGAGAGCTTTCTTAACATTGGTGTCTGGTTCAAGAAGTATTTACCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004177 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016787 GO:0017171 GO:0019538 GO:0022607 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0051259 GO:0051260 GO:0051262 GO:0051289 GO:0065003 GO:0070008 GO:0070009 GO:0070011 GO:0071704 GO:0071840 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PD40 Peptidase_S9

Protein Analysis

618

Amino Acids

66.6

Weight (kDa)

5.76

Isoelectric Point (pI)

41.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
APEH_N	PF19283	2 - 337	1.8e-178	Acylamino-acid-releasing enzyme, N-terminal domain
BD-FAE	PF20434	381 - 569	1.3e-06	BD-FAE
Peptidase_S9	PF00326	400 - 616	1.6e-37	Prolyl oligopeptidase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007453)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G14570
fragaria_vesca	FvH4_4g02060 FvH4_4g02162
malus_domestica	MD13G1203600.v1.1 MD16G1205300.v1.1 MD16G1205600.v1.1
prunus_persica	Prupe.1G026800_v2.0.a1
pyrus_communis	pycom13g17690 pycom16g17300 pycom16g17310
rosa_chinensis	RchiOBHm_Chr4g0389431 RchiOBHm_Chr4g0389461
rosa_laevigata	RLG00000009980 RLG00000009982
rosa_multiflora	Rmu_sc0002498.1_g000017
rosa_roxburghii	Rroxscaffold_5G00335440 Rroxscaffold_5G00335460
rosa_rugosa	Rorug03G0323200
rosa_samantha	Rh4AG027700 Rh4AG027800 Rh4BG020600 Rh4BG021000 Rh4DG021300 Rh4DG021400
rosa_wichuraiana	Rw4G002010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	280, 397, 1447
AccB7I	CCANNNNNTGG	2 cut(s)	614, 1327
AccI	GTMKAC	1 cut(s)	1039
AciI	CCGC	4 cut(s)	219, 954, 1178, 1416
AclWI	GGATC	2 cut(s)	433, 1689
AcoI	YGGCCR	2 cut(s)	605, 1441
AcsI	RAATTY	4 cut(s)	6, 403, 524, 838
AcuI	CTGAAG	3 cut(s)	471, 1064, 1277
AcyI	GRCGYC	1 cut(s)	281
AfaI	GTAC	5 cut(s)	359, 906, 946, 952, 1513
AfiI	CCNNNNNNNGG	3 cut(s)	614, 1305, 1327
AflII	CTTAAG	1 cut(s)	1727
AflIII	ACRYGT	1 cut(s)	1633
AgsI	TTSAA	9 cut(s)	248, 293, 410, 447, 837, 1103, 1206, 1712, 1837
AjiI	CACGTC	3 cut(s)	1327, 1357, 1636
AjnI	CCWGG	3 cut(s)	688, 1320, 1443
AluBI	AGCT	9 cut(s)	59, 233, 346, 440, 472, 771, 825, 1200, 1812
AluI	AGCT	9 cut(s)	59, 233, 346, 440, 472, 771, 825, 1200, 1812
Alw21I	GWGCWC	1 cut(s)	1678
Alw26I	GTCTC	2 cut(s)	1069, 1642
AlwI	GGATC	2 cut(s)	433, 1689
AlwNI	CAGNNNCTG	1 cut(s)	375
Ama87I	CYCGRG	1 cut(s)	1719
AoxI	GGCC	6 cut(s)	371, 605, 1174, 1441, 1545, 1790
ApeKI	GCWGC	3 cut(s)	187, 1088, 1469
ApoI	RAATTY	4 cut(s)	6, 403, 524, 838
AseI	ATTAAT	2 cut(s)	1154, 1502
Asp700I	GAANNNNTTC	3 cut(s)	456, 1138, 1290
AspLEI	GCGC	2 cut(s)	283, 1726
AspS9I	GGNCC	3 cut(s)	372, 1175, 1317
AsuHPI	GGTGA	1 cut(s)	1292
AvaI	CYCGRG	1 cut(s)	1719
AvaII	GGWCC	1 cut(s)	1317
BaeGI	GKGCMC	1 cut(s)	571
BalI	TGGCCA	2 cut(s)	607, 1443
BanI	GGYRCC	3 cut(s)	280, 397, 1447
BbsI	GAAGAC	1 cut(s)	1110
Bbv12I	GWGCWC	1 cut(s)	1678
BbvCI	CCTCAGC	2 cut(s)	48, 672
BbvI	GCAGC	3 cut(s)	199, 1075, 1481
BccI	CCATC	6 cut(s)	512, 608, 671, 728, 857, 1664
BcgI	CGANNNNNNTGC	2 cut(s)	1206, 1240
BciT130I	CCWGG	3 cut(s)	690, 1322, 1445
BciVI	GTATCC	2 cut(s)	719, 1765
BclI	TGATCA	1 cut(s)	747
BcoDI	GTCTC	2 cut(s)	1069, 1642
BfaI	CTAG	3 cut(s)	546, 888, 1304
BfoI	RGCGCY	2 cut(s)	284, 1727
BfrI	CTTAAG	1 cut(s)	1727
BfuI	GTATCC	2 cut(s)	719, 1765
BglII	AGATCT	1 cut(s)	454
BisI	GCNGC	4 cut(s)	188, 1089, 1417, 1470
BlpI	GCTNAGC	1 cut(s)	1593
BlsI	GCNGC	4 cut(s)	189, 1090, 1418, 1471
BmcAI	AGTACT	1 cut(s)	359
Bme1390I	CCNGG	3 cut(s)	690, 1322, 1445
Bme18I	GGWCC	1 cut(s)	1317
BmeT110I	CYCGRG	1 cut(s)	1719
BmgBI	CACGTC	3 cut(s)	1327, 1357, 1636
BmgT120I	GGNCC	3 cut(s)	372, 1175, 1317
BmiI	GGNNCC	8 cut(s)	54, 282, 399, 1050, 1318, 1319, 1449, 1481
BmrFI	CCNGG	3 cut(s)	690, 1322, 1445
BmsI	GCATC	4 cut(s)	310, 728, 1391, 1606
BpiI	GAAGAC	1 cut(s)	1110
Bpu10I	CCTNAGC	4 cut(s)	48, 672, 1213, 1677
Bpu1102I	GCTNAGC	1 cut(s)	1593
Bsa29I	ATCGAT	1 cut(s)	1350
BsaAI	YACGTR	1 cut(s)	818
BsaHI	GRCGYC	1 cut(s)	281
BsaJI	CCNNGG	7 cut(s)	108, 503, 688, 689, 722, 1168, 1320
BsaXI	ACNNNNNCTCC	2 cut(s)	1731, 1761
Bsc4I	CCNNNNNNNGG	3 cut(s)	614, 1305, 1327
Bse3DI	GCAATG	1 cut(s)	876
BseBI	CCWGG	3 cut(s)	690, 1322, 1445
BseCI	ATCGAT	1 cut(s)	1350
BseDI	CCNNGG	7 cut(s)	108, 503, 688, 689, 722, 1168, 1320
BseGI	GGATG	9 cut(s)	619, 682, 743, 853, 868, 1066, 1336, 1521, 1656
BseLI	CCNNNNNNNGG	3 cut(s)	614, 1305, 1327
BseMI	GCAATG	1 cut(s)	876
BseMII	CTCAG	5 cut(s)	39, 663, 990, 1584, 1691
BseRI	GAGGAG	1 cut(s)	1012
BseSI	GKGCMC	1 cut(s)	571
BseXI	GCAGC	3 cut(s)	199, 1075, 1481
BseYI	CCCAGC	1 cut(s)	1270
BsgI	GTGCAG	1 cut(s)	177
BshFI	GGCC	6 cut(s)	373, 607, 1176, 1443, 1547, 1792
BshNI	GGYRCC	3 cut(s)	280, 397, 1447
BshVI	ATCGAT	1 cut(s)	1350
BsiHKAI	GWGCWC	1 cut(s)	1678
BsiHKCI	CYCGRG	1 cut(s)	1719
BslFI	GGGAC	3 cut(s)	881, 1282, 1303
BslI	CCNNNNNNNGG	3 cut(s)	614, 1305, 1327
BsmAI	GTCTC	2 cut(s)	1069, 1642
BsmBI	CGTCTC	1 cut(s)	1642
BsmFI	GGGAC	3 cut(s)	881, 1282, 1303
BsmI	GAATGC	1 cut(s)	998
BsnI	GGCC	6 cut(s)	373, 607, 1176, 1443, 1547, 1792
BsoBI	CYCGRG	1 cut(s)	1719
Bsp1286I	GDGCHC	2 cut(s)	571, 1678
Bsp143I	GATC	6 cut(s)	425, 454, 747, 1351, 1506, 1681
Bsp1720I	GCTNAGC	1 cut(s)	1593
Bsp19I	CCATGG	2 cut(s)	722, 1168
BspACI	CCGC	4 cut(s)	219, 954, 1178, 1416
BspANI	GGCC	6 cut(s)	373, 607, 1176, 1443, 1547, 1792
BspCNI	CTCAG	5 cut(s)	40, 664, 991, 1585, 1690
BspDI	ATCGAT	1 cut(s)	1350
BspLI	GGNNCC	8 cut(s)	54, 282, 399, 1050, 1318, 1319, 1449, 1481
BspPI	GGATC	2 cut(s)	433, 1689
BspT107I	GGYRCC	3 cut(s)	280, 397, 1447
BspTI	CTTAAG	1 cut(s)	1727
BsrDI	GCAATG	1 cut(s)	876
BssECI	CCNNGG	7 cut(s)	108, 503, 688, 689, 722, 1168, 1320
BssMI	GATC	6 cut(s)	425, 454, 747, 1351, 1506, 1681
BssNAI	GTATAC	1 cut(s)	1040
BssNI	GRCGYC	1 cut(s)	281
BssT1I	CCWWGG	2 cut(s)	722, 1168
Bst1107I	GTATAC	1 cut(s)	1040
Bst2UI	CCWGG	3 cut(s)	690, 1322, 1445
Bst4CI	ACNGT	2 cut(s)	507, 1851
Bst6I	CTCTTC	1 cut(s)	594
BstACI	GRCGYC	1 cut(s)	281
BstAFI	CTTAAG	1 cut(s)	1727
BstAPI	GCANNNNNTGC	2 cut(s)	575, 1589
BstBAI	YACGTR	1 cut(s)	818
BstC8I	GCNNGC	3 cut(s)	192, 1178, 1374
BstDEI	CTNAG	7 cut(s)	48, 672, 713, 999, 1213, 1593, 1677
BstDSI	CCRYGG	4 cut(s)	108, 503, 722, 1168
BstEII	GGTNACC	1 cut(s)	923
BstF5I	GGATG	9 cut(s)	619, 682, 743, 853, 868, 1066, 1336, 1521, 1656
BstH2I	RGCGCY	2 cut(s)	284, 1727
BstHHI	GCGC	2 cut(s)	283, 1726
BstKTI	GATC	6 cut(s)	428, 457, 750, 1354, 1509, 1684
BstMAI	GTCTC	2 cut(s)	1069, 1642
BstMBI	GATC	6 cut(s)	425, 454, 747, 1351, 1506, 1681
BstMWI	GCNNNNNNNGC	3 cut(s)	44, 575, 1589
BstNI	CCWGG	3 cut(s)	690, 1322, 1445
BstPI	GGTNACC	1 cut(s)	923
BstSCI	CCNGG	3 cut(s)	688, 1320, 1443
BstSLI	GKGCMC	1 cut(s)	571
BstV1I	GCAGC	3 cut(s)	199, 1075, 1481
BstV2I	GAAGAC	1 cut(s)	1110
BstX2I	RGATCY	3 cut(s)	425, 454, 1681
BstYI	RGATCY	3 cut(s)	425, 454, 1681
BstZ17I	GTATAC	1 cut(s)	1040
Bsu15I	ATCGAT	1 cut(s)	1350
BsuI	GTATCC	2 cut(s)	719, 1765
BsuRI	GGCC	6 cut(s)	373, 607, 1176, 1443, 1547, 1792
BsuTUI	ATCGAT	1 cut(s)	1350
BtgI	CCRYGG	4 cut(s)	108, 503, 722, 1168
BtrI	CACGTC	3 cut(s)	1327, 1357, 1636
BtsCI	GGATG	9 cut(s)	619, 682, 743, 853, 868, 1066, 1336, 1521, 1656
BtsIMutI	CAGTG	3 cut(s)	615, 857, 1384
Cac8I	GCNNGC	3 cut(s)	192, 1178, 1374
CaiI	CAGNNNCTG	1 cut(s)	375
CfoI	GCGC	2 cut(s)	283, 1726
Cfr13I	GGNCC	3 cut(s)	372, 1175, 1317
ClaI	ATCGAT	1 cut(s)	1350
Csp6I	GTAC	5 cut(s)	358, 905, 945, 951, 1512
CviAII	CATG	6 cut(s)	723, 843, 957, 1169, 1412, 1778
CviQI	GTAC	5 cut(s)	358, 905, 945, 951, 1512
DdeI	CTNAG	7 cut(s)	48, 672, 713, 999, 1213, 1593, 1677
DinI	GGCGCC	1 cut(s)	282
DpnI	GATC	6 cut(s)	427, 456, 749, 1353, 1508, 1683
DpnII	GATC	6 cut(s)	425, 454, 747, 1351, 1506, 1681
EaeI	YGGCCR	2 cut(s)	605, 1441
Eam1104I	CTCTTC	1 cut(s)	594
EarI	CTCTTC	1 cut(s)	594
Eco130I	CCWWGG	2 cut(s)	722, 1168
Eco147I	AGGCCT	1 cut(s)	1547
Eco47I	GGWCC	1 cut(s)	1317
Eco57I	CTGAAG	3 cut(s)	471, 1064, 1277
Eco88I	CYCGRG	1 cut(s)	1719
Eco91I	GGTNACC	1 cut(s)	923
EcoO109I	RGGNCCY	2 cut(s)	372, 1317
EcoO65I	GGTNACC	1 cut(s)	923
EcoRII	CCWGG	3 cut(s)	688, 1320, 1443
EcoT14I	CCWWGG	2 cut(s)	722, 1168
EgeI	GGCGCC	1 cut(s)	282
EheI	GGCGCC	1 cut(s)	282
ErhI	CCWWGG	2 cut(s)	722, 1168
Esp3I	CGTCTC	1 cut(s)	1642
FaeI	CATG	6 cut(s)	726, 846, 960, 1172, 1415, 1781
FalI	AAGNNNNNCTT	8 cut(s)	347, 379, 478, 510, 768, 800, 1275, 1307
FaqI	GGGAC	3 cut(s)	881, 1282, 1303
FatI	CATG	6 cut(s)	722, 842, 956, 1168, 1411, 1777
FauI	CCCGC	1 cut(s)	1185
FbaI	TGATCA	1 cut(s)	747
FblI	GTMKAC	1 cut(s)	1039
Fnu4HI	GCNGC	4 cut(s)	188, 1089, 1417, 1470
FokI	GGATG	9 cut(s)	626, 689, 750, 860, 875, 1073, 1343, 1508, 1643
Fsp4HI	GCNGC	4 cut(s)	188, 1089, 1417, 1470
FspBI	CTAG	3 cut(s)	546, 888, 1304
GlaI	GCGC	2 cut(s)	282, 1725
GluI	GCNGC	4 cut(s)	188, 1089, 1417, 1470
GsaI	CCCAGC	1 cut(s)	1274
HaeII	RGCGCY	2 cut(s)	284, 1727
HaeIII	GGCC	6 cut(s)	373, 607, 1176, 1443, 1547, 1792
HhaI	GCGC	2 cut(s)	283, 1726
Hin1I	GRCGYC	1 cut(s)	281
Hin1II	CATG	6 cut(s)	726, 846, 960, 1172, 1415, 1781
Hin6I	GCGC	2 cut(s)	281, 1724
HinP1I	GCGC	2 cut(s)	281, 1724
HindIII	AAGCTT	1 cut(s)	344
HinfI	GANTC	9 cut(s)	296, 410, 475, 560, 584, 652, 833, 1139, 1603
HphI	GGTGA	1 cut(s)	1292
Hpy166II	GTNNAC	1 cut(s)	1040
Hpy188I	TCNGA	9 cut(s)	331, 341, 415, 430, 733, 1000, 1425, 1602, 1801
Hpy188III	TCNNGA	3 cut(s)	293, 1679, 1837
Hpy8I	GTNNAC	1 cut(s)	1040
HpyAV	CCTTC	6 cut(s)	145, 326, 997, 1039, 1310, 1596
HpyCH4III	ACNGT	2 cut(s)	507, 1851
HpyCH4IV	ACGT	4 cut(s)	817, 1326, 1356, 1635
HpyCH4V	TGCA	9 cut(s)	194, 380, 578, 635, 741, 1030, 1382, 1394, 1612
HpyF10VI	GCNNNNNNNGC	3 cut(s)	44, 575, 1589
HpyF3I	CTNAG	7 cut(s)	48, 672, 713, 999, 1213, 1593, 1677
HpySE526I	ACGT	4 cut(s)	817, 1326, 1356, 1635
Hsp92I	GRCGYC	1 cut(s)	281
Hsp92II	CATG	6 cut(s)	726, 846, 960, 1172, 1415, 1781
HspAI	GCGC	2 cut(s)	281, 1724
KasI	GGCGCC	1 cut(s)	280
KflI	GGGWCCC	1 cut(s)	1317
Ksp22I	TGATCA	1 cut(s)	747
Kzo9I	GATC	6 cut(s)	425, 454, 747, 1351, 1506, 1681
LmnI	GCTCC	1 cut(s)	64
Lsp1109I	GCAGC	3 cut(s)	199, 1075, 1481
LweI	GCATC	4 cut(s)	310, 728, 1391, 1606
MaeI	CTAG	3 cut(s)	546, 888, 1304
MaeII	ACGT	4 cut(s)	817, 1326, 1356, 1635
MaeIII	GTNAC	4 cut(s)	125, 923, 1075, 1245
MalI	GATC	6 cut(s)	427, 456, 749, 1353, 1508, 1683
MboI	GATC	6 cut(s)	425, 454, 747, 1351, 1506, 1681
MboII	GAAGA	8 cut(s)	167, 449, 464, 581, 747, 1110, 1295, 1676
MfeI	CAATTG	2 cut(s)	1098, 1230
MflI	RGATCY	3 cut(s)	425, 454, 1681
MhlI	GDGCHC	2 cut(s)	571, 1678
MlsI	TGGCCA	2 cut(s)	607, 1443
MluNI	TGGCCA	2 cut(s)	607, 1443
Mly113I	GGCGCC	1 cut(s)	281
MlyI	GAGTC	3 cut(s)	290, 469, 1597
Mox20I	TGGCCA	2 cut(s)	607, 1443
MroXI	GAANNNNTTC	3 cut(s)	456, 1138, 1290
MscI	TGGCCA	2 cut(s)	607, 1443
MseI	TTAA	8 cut(s)	213, 366, 968, 1008, 1154, 1502, 1728, 1818
MslI	CAYNNNNRTG	2 cut(s)	881, 1112
Msp20I	TGGCCA	2 cut(s)	607, 1443
MspA1I	CMGCKG	1 cut(s)	219
MspCI	CTTAAG	1 cut(s)	1727
MspR9I	CCNGG	3 cut(s)	690, 1322, 1445
MunI	CAATTG	2 cut(s)	1098, 1230
Mva1269I	GAATGC	1 cut(s)	998
MvaI	CCWGG	3 cut(s)	690, 1322, 1445
MwoI	GCNNNNNNNGC	3 cut(s)	44, 575, 1589
NarI	GGCGCC	1 cut(s)	281
NcoI	CCATGG	2 cut(s)	722, 1168
NdeII	GATC	6 cut(s)	425, 454, 747, 1351, 1506, 1681
NlaIII	CATG	6 cut(s)	726, 846, 960, 1172, 1415, 1781
NlaIV	GGNNCC	8 cut(s)	54, 282, 399, 1050, 1318, 1319, 1449, 1481
PasI	CCCWGGG	1 cut(s)	689
PceI	AGGCCT	1 cut(s)	1547
PctI	GAATGC	1 cut(s)	998
PdmI	GAANNNNTTC	3 cut(s)	456, 1138, 1290
PfeI	GAWTC	6 cut(s)	410, 560, 584, 652, 833, 1139
PflMI	CCANNNNNTGG	2 cut(s)	614, 1327
PkrI	GCNGC	4 cut(s)	189, 1090, 1418, 1471
PleI	GAGTC	3 cut(s)	290, 469, 1597
PluTI	GGCGCC	1 cut(s)	284
PpsI	GAGTC	3 cut(s)	290, 469, 1597
Ppu21I	YACGTR	1 cut(s)	818
PpuMI	RGGWCCY	1 cut(s)	1317
PshBI	ATTAAT	2 cut(s)	1154, 1502
Psp5II	RGGWCCY	1 cut(s)	1317
Psp6I	CCWGG	3 cut(s)	688, 1320, 1443
PspEI	GGTNACC	1 cut(s)	923
PspFI	CCCAGC	1 cut(s)	1270
PspGI	CCWGG	3 cut(s)	688, 1320, 1443
PspN4I	GGNNCC	8 cut(s)	54, 282, 399, 1050, 1318, 1319, 1449, 1481
PspPI	GGNCC	3 cut(s)	372, 1175, 1317
PspPPI	RGGWCCY	1 cut(s)	1317
PstNI	CAGNNNCTG	1 cut(s)	375
PsuI	RGATCY	3 cut(s)	425, 454, 1681
RsaI	GTAC	5 cut(s)	359, 906, 946, 952, 1513
RsaNI	GTAC	5 cut(s)	358, 905, 945, 951, 1512
RseI	CAYNNNNRTG	2 cut(s)	881, 1112
SaqAI	TTAA	8 cut(s)	213, 366, 968, 1008, 1154, 1502, 1728, 1818
SatI	GCNGC	4 cut(s)	188, 1089, 1417, 1470
Sau3AI	GATC	6 cut(s)	425, 454, 747, 1351, 1506, 1681
Sau96I	GGNCC	3 cut(s)	372, 1175, 1317
ScaI	AGTACT	1 cut(s)	359
SchI	GAGTC	3 cut(s)	290, 469, 1597
ScrFI	CCNGG	3 cut(s)	690, 1322, 1445
SduI	GDGCHC	2 cut(s)	571, 1678
SfaNI	GCATC	4 cut(s)	310, 728, 1391, 1606
SfoI	GGCGCC	1 cut(s)	282
SinI	GGWCC	1 cut(s)	1317
SmiMI	CAYNNNNRTG	2 cut(s)	881, 1112
SmlI	CTYRAG	1 cut(s)	1727
SmoI	CTYRAG	1 cut(s)	1727
SseBI	AGGCCT	1 cut(s)	1547
SsiI	CCGC	4 cut(s)	219, 954, 1178, 1416
SspDI	GGCGCC	1 cut(s)	280
SspI	AATATT	1 cut(s)	211
SspMI	CTAG	3 cut(s)	546, 888, 1304
StuI	AGGCCT	1 cut(s)	1547
StyD4I	CCNGG	3 cut(s)	688, 1320, 1443
StyI	CCWWGG	2 cut(s)	722, 1168
TaaI	ACNGT	2 cut(s)	507, 1851
TaiI	ACGT	4 cut(s)	820, 1329, 1359, 1638
TaqI	TCGA	1 cut(s)	1350
TatI	WGTACW	2 cut(s)	357, 944
TauI	GCSGC	1 cut(s)	1419
TfiI	GAWTC	6 cut(s)	410, 560, 584, 652, 833, 1139
Tru1I	TTAA	8 cut(s)	213, 366, 968, 1008, 1154, 1502, 1728, 1818
Tru9I	TTAA	8 cut(s)	213, 366, 968, 1008, 1154, 1502, 1728, 1818
TscAI	CASTG	3 cut(s)	615, 864, 1384
TseI	GCWGC	3 cut(s)	187, 1088, 1469
TspDTI	ATGAA	3 cut(s)	39, 831, 1179
TspGWI	ACGGA	1 cut(s)	125
TspRI	CASTG	3 cut(s)	615, 864, 1384
Van91I	CCANNNNNTGG	2 cut(s)	614, 1327
Vha464I	CTTAAG	1 cut(s)	1727
VpaK11BI	GGWCC	1 cut(s)	1317
VspI	ATTAAT	2 cut(s)	1154, 1502
XapI	RAATTY	4 cut(s)	6, 403, 524, 838
XmiI	GTMKAC	1 cut(s)	1039
XmnI	GAANNNNTTC	3 cut(s)	456, 1138, 1290
XspI	CTAG	3 cut(s)	546, 888, 1304
ZrmI	AGTACT	1 cut(s)	359

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.