Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0007453

Physical Location & Seq

Genomic Coordinates Reverse (-)

13404994 .. 13411339

Gene Structure 6346 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom16g17310.9

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2448 bp

ATGAATGGAATAAGTCCGCGTAATAAAATCGTGGCGAGTCAGCTTGCTTCGTCCCACTCCAAGATTCCCTCCTCCTCTTCCTATTTTATCTCACCTTCACACCTTCTTCTCACACCCAGGAGGAGCAGTCGCAGACCCTTTCGCTATTTTCTCTCTACTAAAAGATTGTCAACGCTTTTAGCCATGGGCAGTTCTAAAGATGGTCCAGTGAAGGAGATGCCTCTCGGGGTAGATGCAACAACTGAGGAAGAATATGCATCTCAGTCTAGGTTACTTCAAGACTTCACTAGTATTTCCAGCATTGACAAGGCATGGATTTTTAAATCTGATAGCGGAATTGGGTCTCAGGCAATGTTTTCAATTAGTCAACCGAATCTTTTGGCAAACAAGAGGAAGAAATTCATTCTATCCTCTCATATTTCAAGAGAAAGTAACAATTCTGTAAACTTCCAATGGTCCCCGTTCCCGGTTGAGATGACAGGAGTATCTGTATTTGTTCCATCCCCGTCCGGTGCAAAGCTTTTTGTAGTTCGGAATCCTGAGAACGAATCTCCCTGCCAATTTGAAATTTGGGGCCAAGGTCAATTGGAGAAAGAATTCCACATTCCCCAATCTGTTCATGGTTCAGTTTATGCTGATGGATGGTTTCAGGGAATTTCTTGGAACGCTGATGAAACTCTCATTGCTTATGTTGCTGAGGAACCAGCTCCCTCCAAGCCCACATTTACAGGTCAGGGGTACAAGAAAGGTAGTTCCACGGATAAGGACTTTGGTAACTGGAAAGGTCAAGGGGATTGGAAGGAAGAATGGGGGGAAACCTATGCTGGAAAAAGGCAGCCTGCACTTTTTGTCATCAATATTAACAGTGGAGAGGCACAAGCTGTCAAAGGAATTGAAAAGTCTTTGAGTGTTGGTCAAGTTGTATGGGCGCCACCAGTTAAAGGCTCGCATCAACCTTTGGTTTTTGTTGGGTGGTCAGAAGGTATCAGAAAGCTTGGTATCAAGTACTGCTTTAACAGGCCCTGTGCATTAGCTAGTTCAACTGAAGATCTTCCTGCTGTAAAGCTGACTCAAAGCATAAGTAGTGCTTTCTATCCACGGTTCAGCCCAGATGGAAAATTCCTTTTGTTTTTATCGGCTAGAAGTTCTGTGGATTCTGGGGCACATTCTGCAACAGATTCTCTTCACAGAATAGACTGGCCAGTGGATGGAGTGCTATCCTCATCTGCAAAAATTGTTGATGTGATTCCTGTTGTTATGTGTGCTGAGGATGGTTGCTTCCCTGGGCTTTACTGGTCAAGTATCCTTAGTAATCCATGGCTTTCTGATGGATGCACAATGATCATATCTTCTATCTGGGGTAGCTGTCAAGTGATACTTTCTGTGAATGTTTTGAGTGGCGAAGTATCACGTATCAGCTCTGCTGATTCAAATTCATGGGATGTTCTTACACTGGATGGGGACAATATCATTGCTGTGTCTAGCAGTCCAGTAGATGTACCTCATATCAATTATGGTTACCTTGTTGATAAAGAAAGTACAAGTACCGCATGGAGTTGGTTAAATGTATCAAGCCCCCCAAGTGAATGCTCTGAGAAGGTTAAATCTTTGCTTTCCTCTCTGCAATTCAGTATACTGAAGGTTCCTGTCAGGGATGTTTCTGGTAGCGTACCAAAAGGAGCTACCAAACCAATTGAAGCCATATTTGTGTCTTCCAAAACTAAAAGAAATGATTCGTTTGACCCATTAATTGTAATCCTCCATGGAGGCCCGCACTCTTTTTCATTGTCAAGCTTTTCAAAGTCCTCAGCATTTCTCTCTTCAGTTGGGTTCAGTTTGTTACTTGTAAATTATAGAGGTTCGCTGGGATTTGGTGAAGAAGCACTTCAGTCCCTTCCAGGGAAAATTGGGTCCCAGGACGTGGATGATGTGCTGGTGGCTATCGATCATGTCATTGACAAGGGGCTTGCCAGTCCATCAAAAATTGCAGTTCTTGGTGGTTCTCATGGCGGCTTTCTGACTACTCACTTGATTGGCCAGGCACCAGATAAGTTTGTTGTGGCAGCAGCGAGGAACCCCGCCTGTAATCTCGCATTAATGATCGGTACGACAGACATCCCAGATTGGTGCTATGTGGAGGCCTATGGAAGTGAGGGTAGAAATAACTACACAGAAGCTCCTTCTGCTGAGCATCTGACTCTCTTGCACAGCAAATCTCCTATTTCACACGTCTCAAAGGTCAAAACACCCACCCTTTTTCTTTTGGGTGCTCAGGATCTTCGTGTTCCAGTTTCTACTGGATTTCAATATGCTCGGGCGCTTAAGGAAAAAGGAGTTCCTGTCAAAGTCATTGTGTTTCCACAGGATACTCATGGAATTGAGAGGCCACAATCGGACTTCGAGAGCTTTCTCAACATTGGTGTCTGGTTCAAGAAGTATTTATCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004177 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016787 GO:0017171 GO:0019538 GO:0022607 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0051259 GO:0051260 GO:0051262 GO:0051289 GO:0065003 GO:0070008 GO:0070009 GO:0070011 GO:0071704 GO:0071840 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PD40 Peptidase_S9

Protein Analysis

816

Amino Acids

88.42

Weight (kDa)

7.01

Isoelectric Point (pI)

50.69

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007453)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G14570
fragaria_vesca	FvH4_4g02060 FvH4_4g02162
malus_domestica	MD13G1203600.v1.1 MD16G1205300.v1.1 MD16G1205600.v1.1
prunus_persica	Prupe.1G026800_v2.0.a1
pyrus_communis	pycom13g17690 pycom16g17300 pycom16g17310
rosa_chinensis	RchiOBHm_Chr4g0389431 RchiOBHm_Chr4g0389461
rosa_laevigata	RLG00000009980 RLG00000009982
rosa_multiflora	Rmu_sc0002498.1_g000017
rosa_roxburghii	Rroxscaffold_5G00335440 Rroxscaffold_5G00335460
rosa_rugosa	Rorug03G0323200
rosa_samantha	Rh4AG027700 Rh4AG027800 Rh4BG020600 Rh4BG021000 Rh4DG021300 Rh4DG021400
rosa_wichuraiana	Rw4G002010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	928, 2041
AccB7I	CCANNNNNTGG	2 cut(s)	1208, 1921
AccI	GTMKAC	1 cut(s)	1633
AccII	CGCG	1 cut(s)	19
AciI	CCGC	6 cut(s)	17, 333, 1548, 1772, 2010, 2079
AclWI	GGATC	1 cut(s)	2283
AcoI	YGGCCR	2 cut(s)	1199, 2035
AcsI	RAATTY	6 cut(s)	398, 567, 596, 654, 1118, 1432
AcuI	CTGAAG	4 cut(s)	1065, 1658, 1806, 1871
AcyI	GRCGYC	1 cut(s)	929
AfaI	GTAC	7 cut(s)	740, 1007, 1500, 1540, 1546, 1671, 2107
AfiI	CCNNNNNNNGG	5 cut(s)	466, 941, 1208, 1899, 1921
AflII	CTTAAG	1 cut(s)	2321
AflIII	ACRYGT	1 cut(s)	2227
AhlI	ACTAGT	1 cut(s)	287
AjiI	CACGTC	2 cut(s)	1921, 2230
AjnI	CCWGG	5 cut(s)	116, 1282, 1897, 1914, 2037
Alw21I	GWGCWC	1 cut(s)	2272
Alw26I	GTCTC	2 cut(s)	348, 2236
AlwI	GGATC	1 cut(s)	2283
AlwNI	CAGNNNCTG	1 cut(s)	1023
Ama87I	CYCGRG	2 cut(s)	224, 2313
AoxI	GGCC	7 cut(s)	574, 1019, 1199, 1768, 2035, 2139, 2384
ApeKI	GCWGC	3 cut(s)	835, 2063, 2066
ApoI	RAATTY	6 cut(s)	398, 567, 596, 654, 1118, 1432
AseI	ATTAAT	2 cut(s)	1748, 2096
Asp700I	GAANNNNTTC	5 cut(s)	398, 596, 1050, 1732, 1884
AspLEI	GCGC	2 cut(s)	931, 2320
AspS9I	GGNCC	6 cut(s)	203, 456, 574, 1020, 1769, 1911
AsuC2I	CCSGG	1 cut(s)	467
AsuHPI	GGTGA	2 cut(s)	84, 1886
AvaI	CYCGRG	2 cut(s)	224, 2313
AvaII	GGWCC	3 cut(s)	203, 456, 1911
BaeGI	GKGCMC	1 cut(s)	1165
BaeI	ACNNNNGTAYC	2 cut(s)	468, 501
BalI	TGGCCA	2 cut(s)	1201, 2037
BanI	GGYRCC	2 cut(s)	928, 2041
BbsI	GAAGAC	1 cut(s)	1704
Bbv12I	GWGCWC	1 cut(s)	2272
BbvCI	CCTCAGC	3 cut(s)	696, 1266, 1807
BbvI	GCAGC	3 cut(s)	847, 2075, 2078
BciT130I	CCWGG	5 cut(s)	118, 1284, 1899, 1916, 2039
BciVI	GTATCC	2 cut(s)	1313, 2359
BclI	TGATCA	1 cut(s)	1341
BcnI	CCSGG	1 cut(s)	467
BcoDI	GTCTC	2 cut(s)	348, 2236
BcuI	ACTAGT	1 cut(s)	287
BfaI	CTAG	5 cut(s)	267, 288, 1035, 1140, 1482
BfoI	RGCGCY	2 cut(s)	932, 2321
BfrI	CTTAAG	1 cut(s)	2321
BfuI	GTATCC	2 cut(s)	1313, 2359
BglII	AGATCT	1 cut(s)	1048
BisI	GCNGC	4 cut(s)	836, 2011, 2064, 2067
BlpI	GCTNAGC	1 cut(s)	2187
BlsI	GCNGC	4 cut(s)	837, 2012, 2065, 2068
BmcAI	AGTACT	1 cut(s)	1007
Bme1390I	CCNGG	6 cut(s)	118, 467, 1284, 1899, 1916, 2039
Bme18I	GGWCC	3 cut(s)	203, 456, 1911
BmeT110I	CYCGRG	2 cut(s)	224, 2313
BmgBI	CACGTC	2 cut(s)	1921, 2230
BmgT120I	GGNCC	6 cut(s)	203, 456, 574, 1020, 1769, 1911
BmiI	GGNNCC	9 cut(s)	458, 575, 702, 930, 1644, 1912, 1913, 2043, 2075
BmrFI	CCNGG	6 cut(s)	118, 467, 1284, 1899, 1916, 2039
BmsI	GCATC	6 cut(s)	207, 223, 266, 958, 1322, 2200
BpiI	GAAGAC	1 cut(s)	1704
Bpu10I	CCTNAGC	4 cut(s)	696, 1266, 1807, 2271
Bpu1102I	GCTNAGC	1 cut(s)	2187
BpuMI	CCSGG	1 cut(s)	467
Bsa29I	ATCGAT	1 cut(s)	1944
BsaAI	YACGTR	1 cut(s)	1412
BsaHI	GRCGYC	1 cut(s)	929
BsaI	GGTCTC	1 cut(s)	348
BsaWI	WCCGGW	1 cut(s)	509
BsaXI	ACNNNNNCTCC	8 cut(s)	112, 142, 474, 504, 2161, 2191, 2325, 2355
Bsc4I	CCNNNNNNNGG	5 cut(s)	466, 941, 1208, 1899, 1921
Bse3DI	GCAATG	3 cut(s)	357, 681, 1470
BseBI	CCWGG	5 cut(s)	118, 1284, 1899, 1916, 2039
BseCI	ATCGAT	1 cut(s)	1944
BseLI	CCNNNNNNNGG	5 cut(s)	466, 941, 1208, 1899, 1921
BseMI	GCAATG	3 cut(s)	357, 681, 1470
BseRI	GAGGAG	3 cut(s)	61, 64, 136
BseSI	GKGCMC	1 cut(s)	1165
BseXI	GCAGC	3 cut(s)	847, 2075, 2078
BseYI	CCCAGC	1 cut(s)	1864
BsgI	GTGCAG	1 cut(s)	825
Bsh1236I	CGCG	1 cut(s)	19
BshFI	GGCC	7 cut(s)	576, 1021, 1201, 1770, 2037, 2141, 2386
BshNI	GGYRCC	2 cut(s)	928, 2041
BshVI	ATCGAT	1 cut(s)	1944
BsiHKAI	GWGCWC	1 cut(s)	2272
BsiHKCI	CYCGRG	2 cut(s)	224, 2313
BsiSI	CCGG	2 cut(s)	467, 510
BslFI	GGGAC	5 cut(s)	37, 442, 1475, 1876, 1897
BslI	CCNNNNNNNGG	5 cut(s)	466, 941, 1208, 1899, 1921
BsmAI	GTCTC	2 cut(s)	348, 2236
BsmBI	CGTCTC	1 cut(s)	2236
BsmFI	GGGAC	5 cut(s)	37, 442, 1475, 1876, 1897
BsmI	GAATGC	1 cut(s)	1592
BsnI	GGCC	7 cut(s)	576, 1021, 1201, 1770, 2037, 2141, 2386
Bso31I	GGTCTC	1 cut(s)	348
BsoBI	CYCGRG	2 cut(s)	224, 2313
Bsp1286I	GDGCHC	2 cut(s)	1165, 2272
Bsp143I	GATC	5 cut(s)	1048, 1341, 1945, 2100, 2275
Bsp1720I	GCTNAGC	1 cut(s)	2187
Bsp19I	CCATGG	3 cut(s)	183, 1316, 1762
BspACI	CCGC	6 cut(s)	17, 333, 1548, 1772, 2010, 2079
BspANI	GGCC	7 cut(s)	576, 1021, 1201, 1770, 2037, 2141, 2386
BspDI	ATCGAT	1 cut(s)	1944
BspFNI	CGCG	1 cut(s)	19
BspLI	GGNNCC	9 cut(s)	458, 575, 702, 930, 1644, 1912, 1913, 2043, 2075
BspPI	GGATC	1 cut(s)	2283
BspT107I	GGYRCC	2 cut(s)	928, 2041
BspTI	CTTAAG	1 cut(s)	2321
BspTNI	GGTCTC	1 cut(s)	348
BsrDI	GCAATG	3 cut(s)	357, 681, 1470
BssMI	GATC	5 cut(s)	1048, 1341, 1945, 2100, 2275
BssNAI	GTATAC	1 cut(s)	1634
BssNI	GRCGYC	1 cut(s)	929
BssT1I	CCWWGG	4 cut(s)	183, 577, 1316, 1762
Bst1107I	GTATAC	1 cut(s)	1634
Bst2UI	CCWGG	5 cut(s)	118, 1284, 1899, 1916, 2039
Bst4CI	ACNGT	2 cut(s)	866, 1101
Bst6I	CTCTTC	3 cut(s)	82, 1188, 1825
BstACI	GRCGYC	1 cut(s)	929
BstAFI	CTTAAG	1 cut(s)	2321
BstAPI	GCANNNNNTGC	1 cut(s)	1169
BstBAI	YACGTR	1 cut(s)	1412
BstC8I	GCNNGC	5 cut(s)	45, 840, 947, 1772, 1968
BstDSI	CCRYGG	5 cut(s)	183, 756, 1097, 1316, 1762
BstEII	GGTNACC	1 cut(s)	1517
BstFNI	CGCG	1 cut(s)	19
BstH2I	RGCGCY	2 cut(s)	932, 2321
BstHHI	GCGC	2 cut(s)	931, 2320
BstKTI	GATC	5 cut(s)	1051, 1344, 1948, 2103, 2278
BstMAI	GTCTC	2 cut(s)	348, 2236
BstMBI	GATC	5 cut(s)	1048, 1341, 1945, 2100, 2275
BstMWI	GCNNNNNNNGC	3 cut(s)	692, 1169, 2183
BstNI	CCWGG	5 cut(s)	118, 1284, 1899, 1916, 2039
BstPI	GGTNACC	1 cut(s)	1517
BstSCI	CCNGG	6 cut(s)	116, 465, 1282, 1897, 1914, 2037
BstSLI	GKGCMC	1 cut(s)	1165
BstUI	CGCG	1 cut(s)	19
BstV1I	GCAGC	3 cut(s)	847, 2075, 2078
BstV2I	GAAGAC	1 cut(s)	1704
BstX2I	RGATCY	2 cut(s)	1048, 2275
BstYI	RGATCY	2 cut(s)	1048, 2275
BstZ17I	GTATAC	1 cut(s)	1634
Bsu15I	ATCGAT	1 cut(s)	1944
BsuI	GTATCC	2 cut(s)	1313, 2359
BsuRI	GGCC	7 cut(s)	576, 1021, 1201, 1770, 2037, 2141, 2386
BsuTUI	ATCGAT	1 cut(s)	1944
BtgI	CCRYGG	5 cut(s)	183, 756, 1097, 1316, 1762
BtrI	CACGTC	2 cut(s)	1921, 2230
BtsIMutI	CAGTG	4 cut(s)	213, 871, 1209, 1451
Cac8I	GCNNGC	5 cut(s)	45, 840, 947, 1772, 1968
CaiI	CAGNNNCTG	1 cut(s)	1023
CfoI	GCGC	2 cut(s)	931, 2320
Cfr13I	GGNCC	6 cut(s)	203, 456, 574, 1020, 1769, 1911
ClaI	ATCGAT	1 cut(s)	1944
Csp6I	GTAC	7 cut(s)	739, 1006, 1499, 1539, 1545, 1670, 2106
CviQI	GTAC	7 cut(s)	739, 1006, 1499, 1539, 1545, 1670, 2106
DinI	GGCGCC	1 cut(s)	930
DpnI	GATC	5 cut(s)	1050, 1343, 1947, 2102, 2277
DpnII	GATC	5 cut(s)	1048, 1341, 1945, 2100, 2275
DraI	TTTAAA	1 cut(s)	322
EaeI	YGGCCR	2 cut(s)	1199, 2035
Eam1104I	CTCTTC	3 cut(s)	82, 1188, 1825
EarI	CTCTTC	3 cut(s)	82, 1188, 1825
Eco130I	CCWWGG	4 cut(s)	183, 577, 1316, 1762
Eco147I	AGGCCT	1 cut(s)	2141
Eco31I	GGTCTC	1 cut(s)	348
Eco47I	GGWCC	3 cut(s)	203, 456, 1911
Eco57I	CTGAAG	4 cut(s)	1065, 1658, 1806, 1871
Eco88I	CYCGRG	2 cut(s)	224, 2313
Eco91I	GGTNACC	1 cut(s)	1517
EcoO109I	RGGNCCY	2 cut(s)	1020, 1911
EcoO65I	GGTNACC	1 cut(s)	1517
EcoRI	GAATTC	1 cut(s)	596
EcoRII	CCWGG	5 cut(s)	116, 1282, 1897, 1914, 2037
EcoT14I	CCWWGG	4 cut(s)	183, 577, 1316, 1762
EcoT22I	ATGCAT	1 cut(s)	259
EgeI	GGCGCC	1 cut(s)	930
EheI	GGCGCC	1 cut(s)	930
ErhI	CCWWGG	4 cut(s)	183, 577, 1316, 1762
Esp3I	CGTCTC	1 cut(s)	2236
FalI	AAGNNNNNCTT	8 cut(s)	995, 1027, 1072, 1104, 1362, 1394, 1869, 1901
FaqI	GGGAC	5 cut(s)	37, 442, 1475, 1876, 1897
FauI	CCCGC	2 cut(s)	1779, 2086
FbaI	TGATCA	1 cut(s)	1341
FblI	GTMKAC	1 cut(s)	1633
Fnu4HI	GCNGC	4 cut(s)	836, 2011, 2064, 2067
Fsp4HI	GCNGC	4 cut(s)	836, 2011, 2064, 2067
FspBI	CTAG	5 cut(s)	267, 288, 1035, 1140, 1482
GlaI	GCGC	2 cut(s)	930, 2319
GluI	GCNGC	4 cut(s)	836, 2011, 2064, 2067
GsaI	CCCAGC	1 cut(s)	1868
HaeII	RGCGCY	2 cut(s)	932, 2321
HaeIII	GGCC	7 cut(s)	576, 1021, 1201, 1770, 2037, 2141, 2386
HapII	CCGG	2 cut(s)	467, 510
HhaI	GCGC	2 cut(s)	931, 2320
Hin1I	GRCGYC	1 cut(s)	929
Hin6I	GCGC	2 cut(s)	929, 2318
HinP1I	GCGC	2 cut(s)	929, 2318
HincII	GTYRAC	2 cut(s)	171, 368
HindII	GTYRAC	2 cut(s)	171, 368
HindIII	AAGCTT	3 cut(s)	518, 992, 1792
HpaII	CCGG	2 cut(s)	467, 510
HphI	GGTGA	2 cut(s)	84, 1886
Hpy166II	GTNNAC	4 cut(s)	171, 368, 445, 1634
Hpy188I	TCNGA	9 cut(s)	328, 534, 979, 989, 1327, 1594, 2019, 2196, 2395
Hpy188III	TCNNGA	6 cut(s)	278, 423, 539, 2273, 2401, 2431
Hpy8I	GTNNAC	4 cut(s)	171, 368, 445, 1634
HpyAV	CCTTC	9 cut(s)	105, 113, 205, 793, 974, 1591, 1633, 1904, 2190
HpyCH4III	ACNGT	2 cut(s)	866, 1101
HpyCH4IV	ACGT	3 cut(s)	1411, 1920, 2229
HpyF10VI	GCNNNNNNNGC	3 cut(s)	692, 1169, 2183
HpySE526I	ACGT	3 cut(s)	1411, 1920, 2229
Hsp92I	GRCGYC	1 cut(s)	929
HspAI	GCGC	2 cut(s)	929, 2318
KasI	GGCGCC	1 cut(s)	928
KflI	GGGWCCC	1 cut(s)	1911
Ksp22I	TGATCA	1 cut(s)	1341
Kzo9I	GATC	5 cut(s)	1048, 1341, 1945, 2100, 2275
LmnI	GCTCC	4 cut(s)	123, 712, 1679, 2182
Lsp1109I	GCAGC	3 cut(s)	847, 2075, 2078
LweI	GCATC	6 cut(s)	207, 223, 266, 958, 1322, 2200
MaeI	CTAG	5 cut(s)	267, 288, 1035, 1140, 1482
MaeII	ACGT	3 cut(s)	1411, 1920, 2229
MaeIII	GTNAC	5 cut(s)	270, 431, 773, 1517, 1839
MalI	GATC	5 cut(s)	1050, 1343, 1947, 2102, 2277
MboI	GATC	5 cut(s)	1048, 1341, 1945, 2100, 2275
MfeI	CAATTG	2 cut(s)	584, 1692
MflI	RGATCY	2 cut(s)	1048, 2275
MhlI	GDGCHC	2 cut(s)	1165, 2272
MlsI	TGGCCA	2 cut(s)	1201, 2037
MluNI	TGGCCA	2 cut(s)	1201, 2037
Mly113I	GGCGCC	1 cut(s)	929
MlyI	GAGTC	3 cut(s)	46, 1063, 2191
Mox20I	TGGCCA	2 cut(s)	1201, 2037
Mph1103I	ATGCAT	1 cut(s)	259
MroXI	GAANNNNTTC	5 cut(s)	398, 596, 1050, 1732, 1884
MscI	TGGCCA	2 cut(s)	1201, 2037
MseI	TTAA	9 cut(s)	321, 861, 939, 1014, 1562, 1602, 1748, 2096, 2322
MslI	CAYNNNNRTG	2 cut(s)	1475, 1706
Msp20I	TGGCCA	2 cut(s)	1201, 2037
MspCI	CTTAAG	1 cut(s)	2321
MspI	CCGG	2 cut(s)	467, 510
MspR9I	CCNGG	6 cut(s)	118, 467, 1284, 1899, 1916, 2039
MunI	CAATTG	2 cut(s)	584, 1692
Mva1269I	GAATGC	1 cut(s)	1592
MvaI	CCWGG	5 cut(s)	118, 1284, 1899, 1916, 2039
MvnI	CGCG	1 cut(s)	19
MwoI	GCNNNNNNNGC	3 cut(s)	692, 1169, 2183
NarI	GGCGCC	1 cut(s)	929
NciI	CCSGG	1 cut(s)	467
NcoI	CCATGG	3 cut(s)	183, 1316, 1762
NdeII	GATC	5 cut(s)	1048, 1341, 1945, 2100, 2275
NlaIV	GGNNCC	9 cut(s)	458, 575, 702, 930, 1644, 1912, 1913, 2043, 2075
NsiI	ATGCAT	1 cut(s)	259
PasI	CCCWGGG	1 cut(s)	1283
PceI	AGGCCT	1 cut(s)	2141
PctI	GAATGC	1 cut(s)	1592
PdmI	GAANNNNTTC	5 cut(s)	398, 596, 1050, 1732, 1884
PfeI	GAWTC	9 cut(s)	64, 373, 535, 548, 1154, 1178, 1246, 1427, 1733
PflMI	CCANNNNNTGG	2 cut(s)	1208, 1921
PkrI	GCNGC	4 cut(s)	837, 2012, 2065, 2068
PleI	GAGTC	3 cut(s)	45, 1063, 2191
PluTI	GGCGCC	1 cut(s)	932
PpsI	GAGTC	3 cut(s)	45, 1063, 2191
Ppu21I	YACGTR	1 cut(s)	1412
PpuMI	RGGWCCY	1 cut(s)	1911
PshBI	ATTAAT	2 cut(s)	1748, 2096
Psp5II	RGGWCCY	1 cut(s)	1911
Psp6I	CCWGG	5 cut(s)	116, 1282, 1897, 1914, 2037
PspEI	GGTNACC	1 cut(s)	1517
PspFI	CCCAGC	1 cut(s)	1864
PspGI	CCWGG	5 cut(s)	116, 1282, 1897, 1914, 2037
PspN4I	GGNNCC	9 cut(s)	458, 575, 702, 930, 1644, 1912, 1913, 2043, 2075
PspPI	GGNCC	6 cut(s)	203, 456, 574, 1020, 1769, 1911
PspPPI	RGGWCCY	1 cut(s)	1911
PstNI	CAGNNNCTG	1 cut(s)	1023
PsuI	RGATCY	2 cut(s)	1048, 2275
RsaI	GTAC	7 cut(s)	740, 1007, 1500, 1540, 1546, 1671, 2107
RsaNI	GTAC	7 cut(s)	739, 1006, 1499, 1539, 1545, 1670, 2106
RseI	CAYNNNNRTG	2 cut(s)	1475, 1706
SaqAI	TTAA	9 cut(s)	321, 861, 939, 1014, 1562, 1602, 1748, 2096, 2322
SatI	GCNGC	4 cut(s)	836, 2011, 2064, 2067
Sau3AI	GATC	5 cut(s)	1048, 1341, 1945, 2100, 2275
Sau96I	GGNCC	6 cut(s)	203, 456, 574, 1020, 1769, 1911
ScaI	AGTACT	1 cut(s)	1007
SchI	GAGTC	3 cut(s)	46, 1063, 2191
ScrFI	CCNGG	6 cut(s)	118, 467, 1284, 1899, 1916, 2039
SduI	GDGCHC	2 cut(s)	1165, 2272
SfaNI	GCATC	6 cut(s)	207, 223, 266, 958, 1322, 2200
SfoI	GGCGCC	1 cut(s)	930
SinI	GGWCC	3 cut(s)	203, 456, 1911
SmiMI	CAYNNNNRTG	2 cut(s)	1475, 1706
SmlI	CTYRAG	1 cut(s)	2321
SmoI	CTYRAG	1 cut(s)	2321
SpeI	ACTAGT	1 cut(s)	287
SseBI	AGGCCT	1 cut(s)	2141
SsiI	CCGC	6 cut(s)	17, 333, 1548, 1772, 2010, 2079
SspDI	GGCGCC	1 cut(s)	928
SspI	AATATT	1 cut(s)	859
SspMI	CTAG	5 cut(s)	267, 288, 1035, 1140, 1482
StuI	AGGCCT	1 cut(s)	2141
StyD4I	CCNGG	6 cut(s)	116, 465, 1282, 1897, 1914, 2037
StyI	CCWWGG	4 cut(s)	183, 577, 1316, 1762
TaaI	ACNGT	2 cut(s)	866, 1101
TaiI	ACGT	3 cut(s)	1414, 1923, 2232
TaqI	TCGA	2 cut(s)	1944, 2400
TatI	WGTACW	2 cut(s)	1005, 1538
TauI	GCSGC	1 cut(s)	2013
TfiI	GAWTC	9 cut(s)	64, 373, 535, 548, 1154, 1178, 1246, 1427, 1733
Tru1I	TTAA	9 cut(s)	321, 861, 939, 1014, 1562, 1602, 1748, 2096, 2322
Tru9I	TTAA	9 cut(s)	321, 861, 939, 1014, 1562, 1602, 1748, 2096, 2322
TscAI	CASTG	4 cut(s)	213, 871, 1209, 1458
TseI	GCWGC	3 cut(s)	835, 2063, 2066
TspDTI	ATGAA	6 cut(s)	17, 391, 608, 687, 1425, 1773
TspGWI	ACGGA	1 cut(s)	773
TspRI	CASTG	4 cut(s)	213, 871, 1209, 1458
Van91I	CCANNNNNTGG	2 cut(s)	1208, 1921
Vha464I	CTTAAG	1 cut(s)	2321
VpaK11BI	GGWCC	3 cut(s)	203, 456, 1911
VspI	ATTAAT	2 cut(s)	1748, 2096
XapI	RAATTY	6 cut(s)	398, 567, 596, 654, 1118, 1432
XcmI	CCANNNNNNNNNTGG	1 cut(s)	617
XmiI	GTMKAC	1 cut(s)	1633
XmnI	GAANNNNTTC	5 cut(s)	398, 596, 1050, 1732, 1884
XspI	CTAG	5 cut(s)	267, 288, 1035, 1140, 1482
ZrmI	AGTACT	1 cut(s)	1007
Zsp2I	ATGCAT	1 cut(s)	259

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.