Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0007453

Physical Location & Seq

Genomic Coordinates Reverse (-)

3308826 .. 3521896

Gene Structure 213071 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG021300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2469 bp

ATGGGCAGTGTAGTGGTACTAAGCAAAGCCAATGCCTCTTTGAATTGGTGCGTCAACGCCCTTCTTCCAAGATACCCTCCTTCTCCTCCTATTATATCTCACCTTTCGTCCACTCCCAGACTCTTTCGCTCTCTTCTCTCTACTAAAAGATTCTCAACCATGGATGCTTCTAAAGCTAGCGCATTGAACGAGATGCCTCTTGGAATAGATGAAACAACTGAGGAAGAATATGCTTTGCAGTCCAGGTTACTTCAAGAATTCCATAGTATATCCAGCATTGACAAGGCATGGATAAAACCTGGAAGTGGAAATGGCTCCCAGGCAATGTTTTCACTTAGTCAACCAAATCTTTTGGGAAACAAAATGAGGAAATTTATTTTATCATCTCATATTTCTAAAGAAAGTAGCAACTCGGTAAACTTCAAATGGGGCCCATTTCCTGTTGAGATGACCGGAGTCTCTGTAATTGTTCCATCACCATCAGGTGCAAAGCTTCTTGTAGTTCGTAATCCTGAAAATGACTCTCCCTGTCAATTCGAAATTTGGGGTCCAGCTCAAGTCGAGAAGGAATTCCATATTCCCCAATCTGTCCATGGCTCAGTATATGCTGATGGCTGGTTTCAGGGAATTTCTTGGAACTCTGATGAAACTCTCATCGCTTATGTTGCTGAGGAACCATCTCCCTCCAAGCCGACATTTACATGTCAGGGCTACAAGAAAGGTAGTTCCACTGATAAGGATGTTGGCAACTGGAAAGGCCAAGGGGATTGGATGGAAGATTGGGGGGAAACCTATGCTGGAAAAAGGCAGCCTGCACTTTTTGTCATAAATGTTAACAGTGGAGAGACACGAGCTGTTAAAGGAATTGAAAAGCGTTTGAGTGTTGGCCAAGTTGTGTGGGCCCCACCAATTAGTGGATCCCATCAATATTTGGTTTTTGTTGGGTGGTCAGCAGATACCAGAAAGCTTGGTATTAAGTACTGTTATAACAGGCCCTGTGCATTGTATGCTGTTAAGGCACCACTTCATGAATCAGAAGCTGATGGGACAGAAATCAAAGATAGTTCAACTGATGGTTGTCCTGTGGTAAATCTAACTCAGAGCATTAGTAGTGCTTTCTTTCCACGATTCAGCCCAGATGGAAAATTGCTTTTGTTTCTATCTGCAAAAAGTTCTGTGGATTCTGGGGCCCATTCTGCAACAGATTCTCTTCACAAAATTGATTGGCCAGTGGATGGAGTGCCGTGCTCATCTGTGGAAGTTGTTGATGTGATTCCTGTTGTAATGTGTGCGGATGATGGCTGCTTCCCTGGCCTTTACTGTTCAAGTTTCCTCACTAATCCATGGCTTTCTGATGGATGCACGATGATTATATCTTCCTTCTGGGGCAGCTGTCAAGTGATACTTTCTGTGAATGTGTTGAGCGGACAAGTATCACGCATCAGCCCTACTGATTCAAACTTTTCATGGGATGTTCTTTCACTGGATGGGGACAATATTGTTGCTGTGTCTAGCAGTCCAGTAGATGTGCCTCACATCAAGTATGGTCTTCTTGATGATAAAGAAAGTAAAAGTACTGCATGGAATTGGTTAGATGTACCAAGCCCCACAAATGAATGCTCTGAAAAGGTTAAATCATTGCTCTCATCTCTAAAATTCAGTATATTGAAGATTCCTGTCAGGGATGTTTCTGATAGCCTAACAAAAGGTGCTAGAAAACCTTTTGAAGCTACTTTTGTCTCTTCCAAAACTAAAAGAAGTGATCCATTAGTTGTAGTCCTTCATGGTGGCCCACATTTTGTTTATTTGGCAAGCTTTTCAAAGTCCCTGGCTTTTCTCTCTTCAAATGGGTTCAGTTTGTTACATGTAAATTATAGAGGTTCACTGGGCTTTGGTGAGGAAGCACTTCAATCTCTTCCAGGGAAAGTTGGCTCCCAGGATGTAAATGATGTACTGGTGGCTATTGATCATGTCATTGACTTGGGGCTTGCTAGTCCATCAAAAATTGCAGTTCTTGGTGGTTCTCATGGTGGCTTTCTAACAACTCACTTGATTGGCCAGGCACCAGAGAAGTTTGCTGCGGCTTGTGCAAGGAATCCTGTCTGTAACCTTGCATTGATGGTTGGTACATCAGACATCCCTGATTGGTGCTATCTGGAGGCCTTTGGAAGTGAAGGTAAAAGTATCTATTCAGAAGCACCTTCAGCTGAGGATCTGGCTCTTTTTCACAGCAAGTCTCCTATTTCACATATTTCAAAGGTCAAAACACCCACCCTTTTTCTGTTGGGTGCAAAGGATCTTCGTGTTCCGATTTCCACTGGATTGCAGTATGCTCGGGCATTGAAGGAAAAAGGAGTTGCTGTCAAAGTCATTGTGTTCCCTAATGACATCCATCCAATTGACAGGCCTCAATCCGACTTTGAGATCTTTCTTAATATTGACGTGTGGTTTAAGAAGTATTTGTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004177 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016787 GO:0017171 GO:0019538 GO:0022607 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0051259 GO:0051260 GO:0051262 GO:0051289 GO:0065003 GO:0070008 GO:0070009 GO:0070011 GO:0071704 GO:0071840 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PD40 Peptidase_S9

Protein Analysis

822

Amino Acids

89.25

Weight (kDa)

6.12

Isoelectric Point (pI)

43.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
APEH_N	PF19283	64 - 545	1e-258	Acylamino-acid-releasing enzyme, N-terminal domain
BD-FAE	PF20434	581 - 775	3.5e-08	BD-FAE
Peptidase_S9	PF00326	605 - 821	2.5e-36	Prolyl oligopeptidase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007453)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G14570
fragaria_vesca	FvH4_4g02060 FvH4_4g02162
malus_domestica	MD13G1203600.v1.1 MD16G1205300.v1.1 MD16G1205600.v1.1
prunus_persica	Prupe.1G026800_v2.0.a1
pyrus_communis	pycom13g17690 pycom16g17300 pycom16g17310
rosa_chinensis	RchiOBHm_Chr4g0389431 RchiOBHm_Chr4g0389461
rosa_laevigata	RLG00000009980 RLG00000009982
rosa_multiflora	Rmu_sc0002498.1_g000017
rosa_roxburghii	Rroxscaffold_5G00335440 Rroxscaffold_5G00335460
rosa_rugosa	Rorug03G0323200
rosa_samantha	Rh4AG027700 Rh4AG027800 Rh4BG020600 Rh4BG021000 Rh4DG021300 Rh4DG021400
rosa_wichuraiana	Rw4G002010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	987
AccB1I	GGYRCC	2 cut(s)	1018, 2062
AccB7I	CCANNNNNTGG	2 cut(s)	914, 1235
AccBSI	CCGCTC	1 cut(s)	1425
AciI	CCGC	3 cut(s)	1292, 1425, 2081
AclWI	GGATC	5 cut(s)	912, 925, 1757, 2220, 2304
AcoI	YGGCCR	3 cut(s)	886, 1226, 2056
AcsI	RAATTY	6 cut(s)	257, 371, 540, 569, 627, 1655
AcuI	CTGAAG	1 cut(s)	2187
AfaI	GTAC	6 cut(s)	18, 980, 1576, 1599, 1953, 2128
AfiI	CCNNNNNNNGG	3 cut(s)	305, 914, 1235
AflIII	ACRYGT	3 cut(s)	701, 1864, 2442
AjiI	CACGTC	1 cut(s)	2443
AjnI	CCWGG	8 cut(s)	242, 298, 318, 1309, 1827, 1918, 1935, 2058
Alw21I	GWGCWC	1 cut(s)	1250
Alw26I	GTCTC	4 cut(s)	463, 839, 1744, 2241
AlwI	GGATC	5 cut(s)	912, 925, 1757, 2220, 2304
AlwNI	CAGNNNCTG	2 cut(s)	996, 1040
Ama87I	CYCGRG	1 cut(s)	2334
ApaI	GGGCCC	3 cut(s)	434, 904, 1192
ApeKI	GCWGC	4 cut(s)	808, 1302, 1389, 2078
ApoI	RAATTY	6 cut(s)	257, 371, 540, 569, 627, 1655
Asp700I	GAANNNNTTC	2 cut(s)	569, 1905
AspLEI	GCGC	1 cut(s)	182
AspS9I	GGNCC	9 cut(s)	430, 431, 548, 900, 901, 993, 1188, 1189, 1790
AsuHPI	GGTGA	3 cut(s)	92, 468, 1907
AsuII	TTCGAA	1 cut(s)	537
AsuNHI	GCTAGC	1 cut(s)	176
AvaI	CYCGRG	1 cut(s)	2334
AvaII	GGWCC	1 cut(s)	548
BaeGI	GKGCMC	3 cut(s)	434, 904, 1192
BalI	TGGCCA	3 cut(s)	888, 1228, 2058
BamHI	GGATCC	1 cut(s)	917
BanI	GGYRCC	2 cut(s)	1018, 2062
BanII	GRGCYC	3 cut(s)	434, 904, 1192
BauI	CACGAG	1 cut(s)	849
BbsI	GAAGAC	1 cut(s)	1541
Bbv12I	GWGCWC	1 cut(s)	1250
BbvCI	CCTCAGC	2 cut(s)	669, 2208
BbvI	GCAGC	4 cut(s)	820, 1289, 1401, 2065
BceAI	ACGGC	1 cut(s)	1228
BcgI	CGANNNNNNTGC	2 cut(s)	2315, 2349
BciT130I	CCWGG	8 cut(s)	244, 300, 320, 1311, 1829, 1920, 1937, 2060
BclI	TGATCA	1 cut(s)	1966
BcoDI	GTCTC	4 cut(s)	463, 839, 1744, 2241
BfaI	CTAG	5 cut(s)	177, 1512, 1713, 1992, 2467
BglII	AGATCT	1 cut(s)	2424
BisI	GCNGC	5 cut(s)	809, 1303, 1390, 2079, 2082
BlsI	GCNGC	5 cut(s)	810, 1304, 1391, 2080, 2083
BmcAI	AGTACT	2 cut(s)	980, 1576
Bme1390I	CCNGG	8 cut(s)	244, 300, 320, 1311, 1829, 1920, 1937, 2060
Bme18I	GGWCC	1 cut(s)	548
BmeT110I	CYCGRG	1 cut(s)	2334
BmgBI	CACGTC	1 cut(s)	2443
BmgT120I	GGNCC	9 cut(s)	430, 431, 548, 900, 901, 993, 1188, 1189, 1790
BmrFI	CCNGG	8 cut(s)	244, 300, 320, 1311, 1829, 1920, 1937, 2060
BmrI	ACTGGG	1 cut(s)	1895
BmsI	GCATC	4 cut(s)	154, 183, 1349, 1449
BmtI	GCTAGC	1 cut(s)	180
BmuI	ACTGGG	1 cut(s)	1895
BoxI	GACNNNNGTC	1 cut(s)	455
BpiI	GAAGAC	1 cut(s)	1541
BpmI	CTGGAG	1 cut(s)	2177
Bpu10I	CCTNAGC	2 cut(s)	669, 2208
Bpu14I	TTCGAA	1 cut(s)	537
BpuEI	CTTGAG	1 cut(s)	540
BsaJI	CCNNGG	9 cut(s)	159, 318, 592, 760, 1309, 1343, 1827, 1919, 1935
BsaWI	WCCGGW	1 cut(s)	452
BsaXI	ACNNNNNCTCC	4 cut(s)	447, 477, 2346, 2376
Bsc4I	CCNNNNNNNGG	3 cut(s)	305, 914, 1235
Bse1I	ACTGG	7 cut(s)	755, 1229, 1488, 1520, 1890, 1959, 2323
Bse3DI	GCAATG	2 cut(s)	330, 1637
BseBI	CCWGG	8 cut(s)	244, 300, 320, 1311, 1829, 1920, 1937, 2060
BseDI	CCNNGG	9 cut(s)	159, 318, 592, 760, 1309, 1343, 1827, 1919, 1935
BseLI	CCNNNNNNNGG	3 cut(s)	305, 914, 1235
BseMI	GCAATG	2 cut(s)	330, 1637
BseMII	CTCAG	5 cut(s)	210, 612, 660, 1112, 2199
BseNI	ACTGG	7 cut(s)	755, 1229, 1488, 1520, 1890, 1959, 2323
BseRI	GAGGAG	1 cut(s)	75
BseSI	GKGCMC	3 cut(s)	434, 904, 1192
BseXI	GCAGC	4 cut(s)	820, 1289, 1401, 2065
BsgI	GTGCAG	1 cut(s)	798
BshNI	GGYRCC	2 cut(s)	1018, 2062
BsiHKAI	GWGCWC	1 cut(s)	1250
BsiHKCI	CYCGRG	1 cut(s)	2334
BsiSI	CCGG	1 cut(s)	453
BslFI	GGGAC	3 cut(s)	1060, 1505, 1810
BslI	CCNNNNNNNGG	3 cut(s)	305, 914, 1235
BsmAI	GTCTC	4 cut(s)	463, 839, 1744, 2241
BsmFI	GGGAC	3 cut(s)	1060, 1505, 1810
BsmI	GAATGC	1 cut(s)	1622
BsoBI	CYCGRG	1 cut(s)	2334
Bsp119I	TTCGAA	1 cut(s)	537
Bsp120I	GGGCCC	3 cut(s)	430, 900, 1188
Bsp1286I	GDGCHC	4 cut(s)	434, 904, 1192, 1250
Bsp143I	GATC	6 cut(s)	917, 1762, 1966, 2212, 2296, 2424
Bsp19I	CCATGG	3 cut(s)	159, 592, 1343
BspACI	CCGC	3 cut(s)	1292, 1425, 2081
BspCNI	CTCAG	5 cut(s)	211, 611, 661, 1111, 2200
BspHI	TCATGA	1 cut(s)	1027
BspOI	GCTAGC	1 cut(s)	180
BspPI	GGATC	5 cut(s)	912, 925, 1757, 2220, 2304
BspT104I	TTCGAA	1 cut(s)	537
BspT107I	GGYRCC	2 cut(s)	1018, 2062
BsrBI	CCGCTC	1 cut(s)	1425
BsrDI	GCAATG	2 cut(s)	330, 1637
BsrI	ACTGG	7 cut(s)	755, 1229, 1488, 1520, 1890, 1959, 2323
BssECI	CCNNGG	9 cut(s)	159, 318, 592, 760, 1309, 1343, 1827, 1919, 1935
BssMI	GATC	6 cut(s)	917, 1762, 1966, 2212, 2296, 2424
BssSI	CACGAG	1 cut(s)	849
BssT1I	CCWWGG	4 cut(s)	159, 592, 760, 1343
Bst2BI	CACGAG	1 cut(s)	849
Bst2UI	CCWGG	8 cut(s)	244, 300, 320, 1311, 1829, 1920, 1937, 2060
Bst4CI	ACNGT	3 cut(s)	839, 983, 1322
Bst6I	CTCTTC	5 cut(s)	138, 1215, 1747, 1846, 1920
BstAPI	GCANNNNNTGC	1 cut(s)	1007
BstBI	TTCGAA	1 cut(s)	537
BstC8I	GCNNGC	4 cut(s)	178, 813, 1813, 1989
BstDEI	CTNAG	7 cut(s)	20, 219, 335, 598, 669, 1098, 2208
BstDSI	CCRYGG	3 cut(s)	159, 592, 1343
BstHHI	GCGC	1 cut(s)	182
BstKTI	GATC	6 cut(s)	920, 1765, 1969, 2215, 2299, 2427
BstMAI	GTCTC	4 cut(s)	463, 839, 1744, 2241
BstMBI	GATC	6 cut(s)	917, 1762, 1966, 2212, 2296, 2424
BstMWI	GCNNNNNNNGC	8 cut(s)	173, 665, 1007, 1016, 1196, 1311, 2087, 2204
BstNI	CCWGG	8 cut(s)	244, 300, 320, 1311, 1829, 1920, 1937, 2060
BstNSI	RCATGY	2 cut(s)	705, 1868
BstPAI	GACNNNNGTC	1 cut(s)	455
BstSCI	CCNGG	8 cut(s)	242, 298, 318, 1309, 1827, 1918, 1935, 2058
BstSLI	GKGCMC	3 cut(s)	434, 904, 1192
BstV1I	GCAGC	4 cut(s)	820, 1289, 1401, 2065
BstV2I	GAAGAC	1 cut(s)	1541
BstX2I	RGATCY	4 cut(s)	917, 2212, 2296, 2424
BstYI	RGATCY	4 cut(s)	917, 2212, 2296, 2424
BtgI	CCRYGG	3 cut(s)	159, 592, 1343
BtgZI	GCGATG	1 cut(s)	640
BtrI	CACGTC	1 cut(s)	2443
BtsI	GCAGTG	1 cut(s)	13
BtsIMutI	CAGTG	7 cut(s)	13, 729, 844, 1236, 1481, 1883, 2316
Cac8I	GCNNGC	4 cut(s)	178, 813, 1813, 1989
CaiI	CAGNNNCTG	2 cut(s)	996, 1040
CciI	TCATGA	1 cut(s)	1027
CfoI	GCGC	1 cut(s)	182
Cfr13I	GGNCC	9 cut(s)	430, 431, 548, 900, 901, 993, 1188, 1189, 1790
CseI	GACGC	1 cut(s)	40
Csp6I	GTAC	6 cut(s)	17, 979, 1575, 1598, 1952, 2127
CspCI	CAANNNNNGTGG	2 cut(s)	878, 913
CviQI	GTAC	6 cut(s)	17, 979, 1575, 1598, 1952, 2127
DdeI	CTNAG	7 cut(s)	20, 219, 335, 598, 669, 1098, 2208
DpnI	GATC	6 cut(s)	919, 1764, 1968, 2214, 2298, 2426
DpnII	GATC	6 cut(s)	917, 1762, 1966, 2212, 2296, 2424
EaeI	YGGCCR	3 cut(s)	886, 1226, 2056
Eam1104I	CTCTTC	5 cut(s)	138, 1215, 1747, 1846, 1920
EarI	CTCTTC	5 cut(s)	138, 1215, 1747, 1846, 1920
Eco130I	CCWWGG	4 cut(s)	159, 592, 760, 1343
Eco147I	AGGCCT	2 cut(s)	2162, 2407
Eco24I	GRGCYC	3 cut(s)	434, 904, 1192
Eco47I	GGWCC	1 cut(s)	548
Eco57I	CTGAAG	1 cut(s)	2187
Eco88I	CYCGRG	1 cut(s)	2334
EcoO109I	RGGNCCY	4 cut(s)	430, 901, 993, 1188
EcoRI	GAATTC	2 cut(s)	257, 569
EcoRII	CCWGG	8 cut(s)	242, 298, 318, 1309, 1827, 1918, 1935, 2058
EcoT14I	CCWWGG	4 cut(s)	159, 592, 760, 1343
EcoT38I	GRGCYC	3 cut(s)	434, 904, 1192
ErhI	CCWWGG	4 cut(s)	159, 592, 760, 1343
FalI	AAGNNNNNCTT	2 cut(s)	1389, 1421
FaqI	GGGAC	3 cut(s)	1060, 1505, 1810
FbaI	TGATCA	1 cut(s)	1966
Fnu4HI	GCNGC	5 cut(s)	809, 1303, 1390, 2079, 2082
FriOI	GRGCYC	3 cut(s)	434, 904, 1192
Fsp4HI	GCNGC	5 cut(s)	809, 1303, 1390, 2079, 2082
FspBI	CTAG	5 cut(s)	177, 1512, 1713, 1992, 2467
GlaI	GCGC	1 cut(s)	181
GluI	GCNGC	5 cut(s)	809, 1303, 1390, 2079, 2082
GsuI	CTGGAG	1 cut(s)	2177
HapII	CCGG	1 cut(s)	453
HgaI	GACGC	1 cut(s)	40
HhaI	GCGC	1 cut(s)	182
Hin6I	GCGC	1 cut(s)	180
HinP1I	GCGC	1 cut(s)	180
HincII	GTYRAC	3 cut(s)	55, 341, 835
HindII	GTYRAC	3 cut(s)	55, 341, 835
HindIII	AAGCTT	3 cut(s)	491, 965, 1813
HpaI	GTTAAC	1 cut(s)	835
HpaII	CCGG	1 cut(s)	453
HphI	GGTGA	3 cut(s)	92, 468, 1907
Hpy166II	GTNNAC	6 cut(s)	55, 111, 341, 418, 835, 1883
Hpy188III	TCNNGA	6 cut(s)	254, 512, 562, 1028, 1553, 2156
Hpy8I	GTNNAC	6 cut(s)	55, 111, 341, 418, 835, 1883
HpyAV	CCTTC	8 cut(s)	71, 90, 559, 1390, 1790, 2168, 2211, 2338
HpyCH4III	ACNGT	3 cut(s)	839, 983, 1322
HpyCH4IV	ACGT	1 cut(s)	2442
HpyF10VI	GCNNNNNNNGC	8 cut(s)	173, 665, 1007, 1016, 1196, 1311, 2087, 2204
HpyF3I	CTNAG	7 cut(s)	20, 219, 335, 598, 669, 1098, 2208
HpySE526I	ACGT	1 cut(s)	2442
HspAI	GCGC	1 cut(s)	180
Ksp22I	TGATCA	1 cut(s)	1966
KspAI	GTTAAC	1 cut(s)	835
Kzo9I	GATC	6 cut(s)	917, 1762, 1966, 2212, 2296, 2424
LmnI	GCTCC	2 cut(s)	320, 1937
Lsp1109I	GCAGC	4 cut(s)	820, 1289, 1401, 2065
LweI	GCATC	4 cut(s)	154, 183, 1349, 1449
MaeI	CTAG	5 cut(s)	177, 1512, 1713, 1992, 2467
MaeII	ACGT	1 cut(s)	2442
MaeIII	GTNAC	3 cut(s)	246, 1860, 2105
MalI	GATC	6 cut(s)	919, 1764, 1968, 2214, 2298, 2426
MbiI	CCGCTC	1 cut(s)	1425
MboI	GATC	6 cut(s)	917, 1762, 1966, 2212, 2296, 2424
MfeI	CAATTG	1 cut(s)	2397
MflI	RGATCY	4 cut(s)	917, 2212, 2296, 2424
MhlI	GDGCHC	4 cut(s)	434, 904, 1192, 1250
MlsI	TGGCCA	3 cut(s)	888, 1228, 2058
MluNI	TGGCCA	3 cut(s)	888, 1228, 2058
MlyI	GAGTC	3 cut(s)	114, 465, 515
MmeI	TCCRAC	1 cut(s)	2439
Mox20I	TGGCCA	3 cut(s)	888, 1228, 2058
MroXI	GAANNNNTTC	2 cut(s)	569, 1905
MscI	TGGCCA	3 cut(s)	888, 1228, 2058
MseI	TTAA	7 cut(s)	834, 858, 975, 1014, 1632, 2433, 2451
MslI	CAYNNNNRTG	1 cut(s)	700
Msp20I	TGGCCA	3 cut(s)	888, 1228, 2058
MspA1I	CMGCKG	2 cut(s)	1392, 2207
MspI	CCGG	1 cut(s)	453
MspR9I	CCNGG	8 cut(s)	244, 300, 320, 1311, 1829, 1920, 1937, 2060
MunI	CAATTG	1 cut(s)	2397
Mva1269I	GAATGC	1 cut(s)	1622
MvaI	CCWGG	8 cut(s)	244, 300, 320, 1311, 1829, 1920, 1937, 2060
MwoI	GCNNNNNNNGC	8 cut(s)	173, 665, 1007, 1016, 1196, 1311, 2087, 2204
NcoI	CCATGG	3 cut(s)	159, 592, 1343
NdeII	GATC	6 cut(s)	917, 1762, 1966, 2212, 2296, 2424
NheI	GCTAGC	1 cut(s)	176
NspI	RCATGY	2 cut(s)	705, 1868
NspV	TTCGAA	1 cut(s)	537
PagI	TCATGA	1 cut(s)	1027
PceI	AGGCCT	2 cut(s)	2162, 2407
PciI	ACATGT	2 cut(s)	701, 1864
PctI	GAATGC	1 cut(s)	1622
PdmI	GAANNNNTTC	2 cut(s)	569, 1905
PfeI	GAWTC	9 cut(s)	150, 1031, 1128, 1181, 1205, 1273, 1454, 1672, 2095
PflMI	CCANNNNNTGG	2 cut(s)	914, 1235
PkrI	GCNGC	5 cut(s)	810, 1304, 1391, 2080, 2083
PleI	GAGTC	3 cut(s)	114, 464, 515
PpsI	GAGTC	3 cut(s)	114, 464, 515
PscI	ACATGT	2 cut(s)	701, 1864
PshAI	GACNNNNGTC	1 cut(s)	455
PsiI	TTATAA	1 cut(s)	987
Psp6I	CCWGG	8 cut(s)	242, 298, 318, 1309, 1827, 1918, 1935, 2058
PspGI	CCWGG	8 cut(s)	242, 298, 318, 1309, 1827, 1918, 1935, 2058
PspOMI	GGGCCC	3 cut(s)	430, 900, 1188
PspPI	GGNCC	9 cut(s)	430, 431, 548, 900, 901, 993, 1188, 1189, 1790
PstNI	CAGNNNCTG	2 cut(s)	996, 1040
PsuI	RGATCY	4 cut(s)	917, 2212, 2296, 2424
PvuII	CAGCTG	2 cut(s)	1392, 2207
RsaI	GTAC	6 cut(s)	18, 980, 1576, 1599, 1953, 2128
RsaNI	GTAC	6 cut(s)	17, 979, 1575, 1598, 1952, 2127
RseI	CAYNNNNRTG	1 cut(s)	700
SaqAI	TTAA	7 cut(s)	834, 858, 975, 1014, 1632, 2433, 2451
SatI	GCNGC	5 cut(s)	809, 1303, 1390, 2079, 2082
Sau3AI	GATC	6 cut(s)	917, 1762, 1966, 2212, 2296, 2424
Sau96I	GGNCC	9 cut(s)	430, 431, 548, 900, 901, 993, 1188, 1189, 1790
ScaI	AGTACT	2 cut(s)	980, 1576
SchI	GAGTC	3 cut(s)	114, 465, 515
ScrFI	CCNGG	8 cut(s)	244, 300, 320, 1311, 1829, 1920, 1937, 2060
SduI	GDGCHC	4 cut(s)	434, 904, 1192, 1250
SfaNI	GCATC	4 cut(s)	154, 183, 1349, 1449
SfuI	TTCGAA	1 cut(s)	537
SinI	GGWCC	1 cut(s)	548
SmiMI	CAYNNNNRTG	1 cut(s)	700
SmlI	CTYRAG	1 cut(s)	555
SmoI	CTYRAG	1 cut(s)	555
SseBI	AGGCCT	2 cut(s)	2162, 2407
SsiI	CCGC	3 cut(s)	1292, 1425, 2081
SspI	AATATT	3 cut(s)	929, 1498, 2437
SspMI	CTAG	5 cut(s)	177, 1512, 1713, 1992, 2467
StuI	AGGCCT	2 cut(s)	2162, 2407
StyD4I	CCNGG	8 cut(s)	242, 298, 318, 1309, 1827, 1918, 1935, 2058
StyI	CCWWGG	4 cut(s)	159, 592, 760, 1343
TaaI	ACNGT	3 cut(s)	839, 983, 1322
TaiI	ACGT	1 cut(s)	2445
TaqI	TCGA	2 cut(s)	537, 561
TatI	WGTACW	3 cut(s)	978, 1574, 1951
TauI	GCSGC	1 cut(s)	2084
TfiI	GAWTC	9 cut(s)	150, 1031, 1128, 1181, 1205, 1273, 1454, 1672, 2095
Tru1I	TTAA	7 cut(s)	834, 858, 975, 1014, 1632, 2433, 2451
Tru9I	TTAA	7 cut(s)	834, 858, 975, 1014, 1632, 2433, 2451
TscAI	CASTG	7 cut(s)	13, 736, 844, 1236, 1488, 1890, 2323
TseI	GCWGC	4 cut(s)	808, 1302, 1389, 2078
TspDTI	ATGAA	7 cut(s)	225, 660, 1016, 1044, 1455, 1629, 1772
TspRI	CASTG	7 cut(s)	13, 736, 844, 1236, 1488, 1890, 2323
Van91I	CCANNNNNTGG	2 cut(s)	914, 1235
VpaK11BI	GGWCC	1 cut(s)	548
XapI	RAATTY	6 cut(s)	257, 371, 540, 569, 627, 1655
XceI	RCATGY	2 cut(s)	705, 1868
XcmI	CCANNNNNNNNNTGG	1 cut(s)	590
XmnI	GAANNNNTTC	2 cut(s)	569, 1905
XspI	CTAG	5 cut(s)	177, 1512, 1713, 1992, 2467
ZrmI	AGTACT	2 cut(s)	980, 1576

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.