Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0007453

Physical Location & Seq

Genomic Coordinates Reverse (-)

2829949 .. 2836547

Gene Structure 6599 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00335460.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2406 bp

ATGGATGGATCTAAAGCTGTCCCATTGACAGAGATGCCTCTGGGAATTGATGCAACGACTGAGGATGAATATGCTTTGCAGTCTAGATTACTGCAAGAGTTCACCAGTATATCCAGCTTTGACAAGGCATGGATTAAATCTGACAGTAGAAATGGCTCCCAGTCAATGTTTTCAATTAGTCAACCAAACCTTTTGGCAAACAAGAGGAGGAAATTTGTTCTATCCTCTCACATTTCGAAAGAAAGTAGCAGCTCAGTAAAGTTTGAATGGGCCCCCTTTCCCGTTGAGATGACAGGTGTCTCTGTAATTGTTCCATCACCGTCAGGTTCAAAGCTTCTTGTAGTTCGGAATCCTGAAGCCGAAGCTCCCTGTCAATTTGAAATTTGGGGTCCAGCTCAAGTGGAAAAAGAATTCCATATTCCCCAGTCTGTTCATGGCTCAGTATATGCTGATGAATGGTTTCAGGGAATTTCTTGGAACTCTGATGAAACTCTCATCGCTTATGTTGCTGAGGAGCCATCTCCTCTCAAGCCAACATTTACATGTCAGGGCTACAAGAAAGTTAGTACCATTGATAAGGACTTTCCCAACTGGAAAGGACAAGGGGAATGGGAGGAGGAATGGGGGAAACCTATGTTGGAAAAAGGCAGCCTGCACTTTTTGTCATCAACATTAACAGGTCTAGCACAATTTGGTTTAGTTTCTATGAGTACTAGGACTAGATGTAAAGAGATCTCTTCTTCAGAAGGAAAACTTATTTTGAATTTCACAATGCTATATAGTGGAGACACACAAGCGGTTAAAGGAATTGAAAAGCATTTGAGTGTTGGCCAAGTTGTATGGGCACCACCAGTTAGAGGATCACATCAGTATTTGGTTTTTGTTGGGTGGTCAGCAGATACCAGAAAGCTCGGAATTAAGTACTGTTTTAACAGGCCCTGTGCTTTGTATGCGTTTAAGGCACCACTTTATGGATCAGAAGCTGACGGGCAAGAGATCAATTCCAGAGATAATTCAATTAAAGATTCTCCTGTGGTAAATCTAACTGAAAGCATAAGTAGTGCCTACCATCCACGTTTCAGTGCAGATGGAAAATTGCTTTTGTTTTTATCTTCAAGAAGTGCTGTGGATTCTGGGGCACATTCTGCAACAGATTCTCTGCACAGAATTGATTGGCCAATAGATGGGGTGCCATGCTCATCTTTTATAATTTTTGATGTGATTCCTGTTGTAATGTGTGCGGATGATGGTTGCTTCCTGGCCTTTACTGTTCAAGTTTCCTTAGTAATCCATGGTTTTCTGATGGATGCACGATGGTCGTATCTTCCTTCTGGGGCAGCTGTCAATGGAGAGGTACTACGCATCAGCCCTACTAATTCAAACTTTTCATGGAATGTTCTTTCATTGGATGGGGACAACATCATTGCTGTGTCTAGCAGTCCAGTAAATGTACCACGCATCAAGTATGGTTATCTTGTTGATAGACAGAAGAAAAGCACTGCATGGAATTGGTTAGATGTACCAAGCCCCACAAACGAATGCTCTGAGAAGGTGGAATCTTTGCTCTCATCTCTTCAGTTCAGTATAATGAGCATTCCTGTCGGGGATGTTTCTGATAGCCTAACAAAAGGTGCTGGAAAACCTTTTGAGGCTATATTTGTGTCTTCCAAAACTAAAAGAAGTGATTCATTTGATCCATTAATTGTATTCCTTCATGGAGGCCCGCATACTGTTTCTTTGTCAAGCTTTTCAAAGTCCTTAGCATTTGTCTCTACAATTGGGTTCAACTTGTTACTTGTAAATTATAGAGGTTCACTGGGATTTGGTGAGGAAGCACTTCAATCTCTTCCAGGGAATATTGGCTCCCAGGATGTGAATGATGTACTTGTGGCTGTTGATCATGTCATTGACATGGGGCTCGCTAGTCCATCCAAAATTGCCGTTCTTGGTGGTTCTCATGGTGGCTTTTTAACAACTCACTTAATTGGTCAGGCACCGGAGAAGTTTTTTGTGGCAGCTACAAGGAATCCTGCATGTAACCTGGCATTGATGGTTGGTACAACAGACATCCCTGATTGGTGCTATGTGGAGGCCTATGGAACTGAGGGCAAAAATAGATATACAGAAGCACCTTCAGCTGAGCTTCTGGCTCTCTTTCACAGCAAGTCTCCTATTTCACAAATTTCAAAGGCCAAAACACCCACCCTTTTTCTCTTGGGTGCTCAAGATATTCGCCTTCCAATTTGTAGCGGATTGCAATATGCTCGGGCATTAAAGGAAAAGGGAGTTGCTGTGAAAGTCATTGTGTTCCCTAATGACAACCATCCAATTGACAAGCCGCAATCCGACTTTGAGAGCTTTCTAAATATCGGGCAAATACTAATATACCGTGAAGGAGCTGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004177 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016787 GO:0017171 GO:0019538 GO:0022607 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0051259 GO:0051260 GO:0051262 GO:0051289 GO:0065003 GO:0070008 GO:0070009 GO:0070011 GO:0071704 GO:0071840 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PD40 Peptidase_S9

Protein Analysis

801

Amino Acids

87.43

Weight (kDa)

5.71

Isoelectric Point (pI)

42.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
APEH_N	PF19283	11 - 212	8.6e-99	Acylamino-acid-releasing enzyme, N-terminal domain
APEH_N	PF19283	258 - 519	3.4e-103	Acylamino-acid-releasing enzyme, N-terminal domain
COesterase	PF00135	552 - 661	2.8e-06	Carboxylesterase family
Peptidase_S9	PF00326	582 - 784	7.7e-32	Prolyl oligopeptidase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007453)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G14570
fragaria_vesca	FvH4_4g02060 FvH4_4g02162
malus_domestica	MD13G1203600.v1.1 MD16G1205300.v1.1 MD16G1205600.v1.1
prunus_persica	Prupe.1G026800_v2.0.a1
pyrus_communis	pycom13g17690 pycom16g17300 pycom16g17310
rosa_chinensis	RchiOBHm_Chr4g0389431 RchiOBHm_Chr4g0389461
rosa_laevigata	RLG00000009980 RLG00000009982
rosa_multiflora	Rmu_sc0002498.1_g000017
rosa_roxburghii	Rroxscaffold_5G00335440 Rroxscaffold_5G00335460
rosa_rugosa	Rorug03G0323200
rosa_samantha	Rh4AG027700 Rh4AG027800 Rh4BG020600 Rh4BG021000 Rh4DG021300 Rh4DG021400
rosa_wichuraiana	Rw4G002010

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1208
AccB1I	GGYRCC	4 cut(s)	844, 961, 1189, 1993
AccB7I	CCANNNNNTGG	2 cut(s)	971, 1184
AciI	CCGC	5 cut(s)	797, 1241, 1724, 2250, 2339
AclWI	GGATC	4 cut(s)	16, 868, 982, 1688
AcoI	YGGCCR	2 cut(s)	829, 1175
AcsI	RAATTY	6 cut(s)	212, 381, 410, 468, 763, 2181
AcuI	CTGAAG	4 cut(s)	375, 726, 1559, 2118
AfaI	GTAC	8 cut(s)	568, 712, 923, 1356, 1452, 1521, 1884, 2059
AfiI	CCNNNNNNNGG	3 cut(s)	857, 971, 1184
AflIII	ACRYGT	1 cut(s)	542
AjnI	CCWGG	4 cut(s)	1257, 1849, 1866, 2040
AjuI	GAANNNNNNNTTGG	2 cut(s)	620, 652
Alw21I	GWGCWC	1 cut(s)	2224
Alw26I	GTCTC	4 cut(s)	304, 780, 1774, 2172
AlwI	GGATC	4 cut(s)	16, 868, 982, 1688
AlwNI	CAGNNNCTG	2 cut(s)	939, 983
Ama87I	CYCGRG	1 cut(s)	2265
AoxI	GGCC	8 cut(s)	270, 829, 935, 1175, 1260, 1720, 2091, 2190
ApaI	GGGCCC	1 cut(s)	274
ApeKI	GCWGC	4 cut(s)	249, 648, 1337, 2015
ApoI	RAATTY	6 cut(s)	212, 381, 410, 468, 763, 2181
AseI	ATTAAT	1 cut(s)	1700
Asp700I	GAANNNNTTC	3 cut(s)	459, 1684, 1836
AspS9I	GGNCC	5 cut(s)	270, 271, 389, 936, 1721
AsuHPI	GGTGA	3 cut(s)	94, 309, 1838
AsuII	TTCGAA	1 cut(s)	236
AvaI	CYCGRG	1 cut(s)	2265
AvaII	GGWCC	1 cut(s)	389
BaeGI	GKGCMC	3 cut(s)	274, 847, 1141
BalI	TGGCCA	2 cut(s)	831, 1177
BanI	GGYRCC	4 cut(s)	844, 961, 1189, 1993
BanII	GRGCYC	2 cut(s)	274, 1920
BbsI	GAAGAC	1 cut(s)	1656
Bbv12I	GWGCWC	1 cut(s)	2224
BbvCI	CCTCAGC	1 cut(s)	510
BbvI	GCAGC	4 cut(s)	261, 660, 1349, 2027
BceAI	ACGGC	1 cut(s)	1925
BcgI	CGANNNNNNTGC	6 cut(s)	1299, 1333, 1582, 1616, 2246, 2280
BciT130I	CCWGG	4 cut(s)	1259, 1851, 1868, 2042
BclI	TGATCA	1 cut(s)	1897
BcoDI	GTCTC	4 cut(s)	304, 780, 1774, 2172
BfaI	CTAG	6 cut(s)	84, 683, 714, 720, 1434, 1923
BglII	AGATCT	1 cut(s)	732
BisI	GCNGC	5 cut(s)	250, 649, 1338, 2016, 2339
BlpI	GCTNAGC	1 cut(s)	2139
BlsI	GCNGC	5 cut(s)	251, 650, 1339, 2017, 2340
BmcAI	AGTACT	2 cut(s)	712, 923
Bme1390I	CCNGG	4 cut(s)	1259, 1851, 1868, 2042
Bme18I	GGWCC	1 cut(s)	389
BmeT110I	CYCGRG	1 cut(s)	2265
BmgT120I	GGNCC	5 cut(s)	270, 271, 389, 936, 1721
BmrFI	CCNGG	4 cut(s)	1259, 1851, 1868, 2042
BmrI	ACTGGG	3 cut(s)	154, 418, 1826
BmsI	GCATC	5 cut(s)	24, 40, 1297, 1371, 1467
BmuI	ACTGGG	3 cut(s)	154, 418, 1826
BoxI	GACNNNNGTC	1 cut(s)	296
BpiI	GAAGAC	1 cut(s)	1656
Bpu10I	CCTNAGC	2 cut(s)	510, 1759
Bpu1102I	GCTNAGC	1 cut(s)	2139
Bpu14I	TTCGAA	1 cut(s)	236
BpuEI	CTTGAG	3 cut(s)	381, 512, 2208
BsaJI	CCNNGG	3 cut(s)	1291, 1850, 1866
BsaWI	WCCGGW	1 cut(s)	1996
BsaXI	ACNNNNNCTCC	2 cut(s)	2277, 2307
Bsc4I	CCNNNNNNNGG	3 cut(s)	857, 971, 1184
Bse1I	ACTGG	7 cut(s)	105, 160, 424, 596, 851, 1442, 1821
Bse3DI	GCAATG	1 cut(s)	1422
BseBI	CCWGG	4 cut(s)	1259, 1851, 1868, 2042
BseDI	CCNNGG	3 cut(s)	1291, 1850, 1866
BseLI	CCNNNNNNNGG	3 cut(s)	857, 971, 1184
BseMI	GCAATG	1 cut(s)	1422
BseMII	CTCAG	7 cut(s)	51, 267, 453, 501, 1536, 2094, 2130
BseNI	ACTGG	7 cut(s)	105, 160, 424, 596, 851, 1442, 1821
BseRI	GAGGAG	4 cut(s)	220, 513, 527, 629
BseSI	GKGCMC	3 cut(s)	274, 847, 1141
BseXI	GCAGC	4 cut(s)	261, 660, 1349, 2027
BsgI	GTGCAG	3 cut(s)	638, 1104, 1145
BshFI	GGCC	8 cut(s)	272, 831, 937, 1177, 1262, 1722, 2093, 2192
BshNI	GGYRCC	4 cut(s)	844, 961, 1189, 1993
BsiHKAI	GWGCWC	1 cut(s)	2224
BsiHKCI	CYCGRG	1 cut(s)	2265
BsiSI	CCGG	1 cut(s)	1997
BslFI	GGGAC	2 cut(s)	5, 1427
BslI	CCNNNNNNNGG	3 cut(s)	857, 971, 1184
BsmAI	GTCTC	4 cut(s)	304, 780, 1774, 2172
BsmFI	GGGAC	2 cut(s)	5, 1427
BsmI	GAATGC	2 cut(s)	1544, 1593
BsnI	GGCC	8 cut(s)	272, 831, 937, 1177, 1262, 1722, 2093, 2192
BsoBI	CYCGRG	1 cut(s)	2265
Bsp119I	TTCGAA	1 cut(s)	236
Bsp120I	GGGCCC	1 cut(s)	270
Bsp1286I	GDGCHC	5 cut(s)	274, 847, 1141, 1920, 2224
Bsp143I	GATC	7 cut(s)	8, 732, 860, 974, 996, 1693, 1897
Bsp1720I	GCTNAGC	1 cut(s)	2139
Bsp19I	CCATGG	1 cut(s)	1291
BspACI	CCGC	5 cut(s)	797, 1241, 1724, 2250, 2339
BspANI	GGCC	8 cut(s)	272, 831, 937, 1177, 1262, 1722, 2093, 2192
BspCNI	CTCAG	7 cut(s)	52, 266, 452, 502, 1537, 2095, 2131
BspPI	GGATC	4 cut(s)	16, 868, 982, 1688
BspT104I	TTCGAA	1 cut(s)	236
BspT107I	GGYRCC	4 cut(s)	844, 961, 1189, 1993
BsrDI	GCAATG	1 cut(s)	1422
BsrI	ACTGG	7 cut(s)	105, 160, 424, 596, 851, 1442, 1821
BssECI	CCNNGG	3 cut(s)	1291, 1850, 1866
BssMI	GATC	7 cut(s)	8, 732, 860, 974, 996, 1693, 1897
BssT1I	CCWWGG	1 cut(s)	1291
Bst2UI	CCWGG	4 cut(s)	1259, 1851, 1868, 2042
Bst4CI	ACNGT	6 cut(s)	146, 321, 926, 1270, 1732, 2390
Bst6I	CTCTTC	3 cut(s)	742, 1578, 1851
BstAPI	GCANNNNNTGC	1 cut(s)	1145
BstBI	TTCGAA	1 cut(s)	236
BstC8I	GCNNGC	3 cut(s)	653, 1724, 1920
BstDEI	CTNAG	9 cut(s)	60, 253, 439, 510, 1282, 1545, 1759, 2103, 2139
BstDSI	CCRYGG	1 cut(s)	1291
BstKTI	GATC	7 cut(s)	11, 735, 863, 977, 999, 1696, 1900
BstMAI	GTCTC	4 cut(s)	304, 780, 1774, 2172
BstMBI	GATC	7 cut(s)	8, 732, 860, 974, 996, 1693, 1897
BstMWI	GCNNNNNNNGC	5 cut(s)	506, 950, 959, 1145, 2135
BstNI	CCWGG	4 cut(s)	1259, 1851, 1868, 2042
BstNSI	RCATGY	2 cut(s)	546, 2037
BstPAI	GACNNNNGTC	1 cut(s)	296
BstSCI	CCNGG	4 cut(s)	1257, 1849, 1866, 2040
BstSLI	GKGCMC	3 cut(s)	274, 847, 1141
BstV1I	GCAGC	4 cut(s)	261, 660, 1349, 2027
BstV2I	GAAGAC	1 cut(s)	1656
BstX2I	RGATCY	2 cut(s)	8, 732
BstYI	RGATCY	2 cut(s)	8, 732
BsuRI	GGCC	8 cut(s)	272, 831, 937, 1177, 1262, 1722, 2093, 2192
BtgI	CCRYGG	1 cut(s)	1291
BtgZI	GCGATG	1 cut(s)	481
BtsI	GCAGTG	1 cut(s)	1497
BtsIMutI	CAGTG	3 cut(s)	1087, 1497, 1814
Cac8I	GCNNGC	3 cut(s)	653, 1724, 1920
CaiI	CAGNNNCTG	2 cut(s)	939, 983
Cfr13I	GGNCC	5 cut(s)	270, 271, 389, 936, 1721
Csp6I	GTAC	8 cut(s)	567, 711, 922, 1355, 1451, 1520, 1883, 2058
CviQI	GTAC	8 cut(s)	567, 711, 922, 1355, 1451, 1520, 1883, 2058
DdeI	CTNAG	9 cut(s)	60, 253, 439, 510, 1282, 1545, 1759, 2103, 2139
DpnI	GATC	7 cut(s)	10, 734, 862, 976, 998, 1695, 1899
DpnII	GATC	7 cut(s)	8, 732, 860, 974, 996, 1693, 1897
EaeI	YGGCCR	2 cut(s)	829, 1175
Eam1104I	CTCTTC	3 cut(s)	742, 1578, 1851
EarI	CTCTTC	3 cut(s)	742, 1578, 1851
Eco130I	CCWWGG	1 cut(s)	1291
Eco147I	AGGCCT	1 cut(s)	2093
Eco24I	GRGCYC	2 cut(s)	274, 1920
Eco47I	GGWCC	1 cut(s)	389
Eco57I	CTGAAG	4 cut(s)	375, 726, 1559, 2118
Eco88I	CYCGRG	1 cut(s)	2265
EcoO109I	RGGNCCY	2 cut(s)	271, 936
EcoRI	GAATTC	1 cut(s)	410
EcoRII	CCWGG	4 cut(s)	1257, 1849, 1866, 2040
EcoT14I	CCWWGG	1 cut(s)	1291
EcoT38I	GRGCYC	2 cut(s)	274, 1920
ErhI	CCWWGG	1 cut(s)	1291
FalI	AAGNNNNNCTT	2 cut(s)	738, 770
FaqI	GGGAC	2 cut(s)	5, 1427
FauI	CCCGC	1 cut(s)	1731
FbaI	TGATCA	1 cut(s)	1897
Fnu4HI	GCNGC	5 cut(s)	250, 649, 1338, 2016, 2339
FriOI	GRGCYC	2 cut(s)	274, 1920
Fsp4HI	GCNGC	5 cut(s)	250, 649, 1338, 2016, 2339
FspBI	CTAG	6 cut(s)	84, 683, 714, 720, 1434, 1923
GluI	GCNGC	5 cut(s)	250, 649, 1338, 2016, 2339
HaeIII	GGCC	8 cut(s)	272, 831, 937, 1177, 1262, 1722, 2093, 2192
HapII	CCGG	1 cut(s)	1997
HincII	GTYRAC	1 cut(s)	182
HindII	GTYRAC	1 cut(s)	182
HindIII	AAGCTT	2 cut(s)	332, 1744
HinfI	GANTC	8 cut(s)	349, 1025, 1130, 1154, 1222, 1556, 1685, 2026
HpaII	CCGG	1 cut(s)	1997
HphI	GGTGA	3 cut(s)	94, 309, 1838
Hpy166II	GTNNAC	3 cut(s)	102, 182, 1814
Hpy188III	TCNNGA	5 cut(s)	84, 353, 1005, 1116, 2225
Hpy8I	GTNNAC	3 cut(s)	102, 182, 1814
HpyAV	CCTTC	7 cut(s)	740, 1338, 1543, 1721, 2142, 2246, 2387
HpyCH4III	ACNGT	6 cut(s)	146, 321, 926, 1270, 1732, 2390
HpyCH4IV	ACGT	1 cut(s)	1075
HpyF10VI	GCNNNNNNNGC	5 cut(s)	506, 950, 959, 1145, 2135
HpyF3I	CTNAG	9 cut(s)	60, 253, 439, 510, 1282, 1545, 1759, 2103, 2139
HpySE526I	ACGT	1 cut(s)	1075
Ksp22I	TGATCA	1 cut(s)	1897
Kzo9I	GATC	7 cut(s)	8, 732, 860, 974, 996, 1693, 1897
LmnI	GCTCC	5 cut(s)	161, 370, 514, 1868, 2396
Lsp1109I	GCAGC	4 cut(s)	261, 660, 1349, 2027
LweI	GCATC	5 cut(s)	24, 40, 1297, 1371, 1467
MaeI	CTAG	6 cut(s)	84, 683, 714, 720, 1434, 1923
MaeII	ACGT	1 cut(s)	1075
MaeIII	GTNAC	2 cut(s)	1791, 2036
MalI	GATC	7 cut(s)	10, 734, 862, 976, 998, 1695, 1899
MboI	GATC	7 cut(s)	8, 732, 860, 974, 996, 1693, 1897
MboII	GAAGA	8 cut(s)	729, 732, 1104, 1316, 1501, 1565, 1656, 1838
MfeI	CAATTG	2 cut(s)	1776, 2328
MflI	RGATCY	2 cut(s)	8, 732
MhlI	GDGCHC	5 cut(s)	274, 847, 1141, 1920, 2224
MlsI	TGGCCA	2 cut(s)	831, 1177
MluNI	TGGCCA	2 cut(s)	831, 1177
MmeI	TCCRAC	2 cut(s)	618, 2370
Mox20I	TGGCCA	2 cut(s)	831, 1177
MroXI	GAANNNNTTC	3 cut(s)	459, 1684, 1836
MscI	TGGCCA	2 cut(s)	831, 1177
MslI	CAYNNNNRTG	4 cut(s)	541, 822, 1427, 1910
Msp20I	TGGCCA	2 cut(s)	831, 1177
MspA1I	CMGCKG	2 cut(s)	1340, 2138
MspI	CCGG	1 cut(s)	1997
MspR9I	CCNGG	4 cut(s)	1259, 1851, 1868, 2042
MunI	CAATTG	2 cut(s)	1776, 2328
Mva1269I	GAATGC	2 cut(s)	1544, 1593
MvaI	CCWGG	4 cut(s)	1259, 1851, 1868, 2042
MwoI	GCNNNNNNNGC	5 cut(s)	506, 950, 959, 1145, 2135
NcoI	CCATGG	1 cut(s)	1291
NdeII	GATC	7 cut(s)	8, 732, 860, 974, 996, 1693, 1897
NspI	RCATGY	2 cut(s)	546, 2037
NspV	TTCGAA	1 cut(s)	236
PceI	AGGCCT	1 cut(s)	2093
PciI	ACATGT	1 cut(s)	542
PctI	GAATGC	2 cut(s)	1544, 1593
PdmI	GAANNNNTTC	3 cut(s)	459, 1684, 1836
PfeI	GAWTC	8 cut(s)	349, 1025, 1130, 1154, 1222, 1556, 1685, 2026
PflMI	CCANNNNNTGG	2 cut(s)	971, 1184
PkrI	GCNGC	5 cut(s)	251, 650, 1339, 2017, 2340
PscI	ACATGT	1 cut(s)	542
PshAI	GACNNNNGTC	1 cut(s)	296
PshBI	ATTAAT	1 cut(s)	1700
PsiI	TTATAA	1 cut(s)	1208
Psp6I	CCWGG	4 cut(s)	1257, 1849, 1866, 2040
PspGI	CCWGG	4 cut(s)	1257, 1849, 1866, 2040
PspOMI	GGGCCC	1 cut(s)	270
PspPI	GGNCC	5 cut(s)	270, 271, 389, 936, 1721
PstNI	CAGNNNCTG	2 cut(s)	939, 983
PsuI	RGATCY	2 cut(s)	8, 732
PvuII	CAGCTG	2 cut(s)	1340, 2138
RsaI	GTAC	8 cut(s)	568, 712, 923, 1356, 1452, 1521, 1884, 2059
RsaNI	GTAC	8 cut(s)	567, 711, 922, 1355, 1451, 1520, 1883, 2058
RseI	CAYNNNNRTG	4 cut(s)	541, 822, 1427, 1910
SatI	GCNGC	5 cut(s)	250, 649, 1338, 2016, 2339
Sau3AI	GATC	7 cut(s)	8, 732, 860, 974, 996, 1693, 1897
Sau96I	GGNCC	5 cut(s)	270, 271, 389, 936, 1721
ScaI	AGTACT	2 cut(s)	712, 923
ScrFI	CCNGG	4 cut(s)	1259, 1851, 1868, 2042
SduI	GDGCHC	5 cut(s)	274, 847, 1141, 1920, 2224
SfaNI	GCATC	5 cut(s)	24, 40, 1297, 1371, 1467
SfuI	TTCGAA	1 cut(s)	236
SinI	GGWCC	1 cut(s)	389
SmiMI	CAYNNNNRTG	4 cut(s)	541, 822, 1427, 1910
SmlI	CTYRAG	3 cut(s)	396, 527, 2223
SmoI	CTYRAG	3 cut(s)	396, 527, 2223
SseBI	AGGCCT	1 cut(s)	2093
SsiI	CCGC	5 cut(s)	797, 1241, 1724, 2250, 2339
SspI	AATATT	1 cut(s)	1858
SspMI	CTAG	6 cut(s)	84, 683, 714, 720, 1434, 1923
StuI	AGGCCT	1 cut(s)	2093
StyD4I	CCNGG	4 cut(s)	1257, 1849, 1866, 2040
StyI	CCWWGG	1 cut(s)	1291
TaaI	ACNGT	6 cut(s)	146, 321, 926, 1270, 1732, 2390
TaiI	ACGT	1 cut(s)	1078
TaqI	TCGA	1 cut(s)	236
TatI	WGTACW	3 cut(s)	710, 921, 1882
TauI	GCSGC	1 cut(s)	2341
TfiI	GAWTC	8 cut(s)	349, 1025, 1130, 1154, 1222, 1556, 1685, 2026
TscAI	CASTG	3 cut(s)	1087, 1504, 1821
TseI	GCWGC	4 cut(s)	249, 648, 1337, 2015
TspDTI	ATGAA	8 cut(s)	81, 422, 468, 501, 1377, 1392, 1677, 1703
TspRI	CASTG	3 cut(s)	1087, 1504, 1821
Van91I	CCANNNNNTGG	2 cut(s)	971, 1184
VpaK11BI	GGWCC	1 cut(s)	389
VspI	ATTAAT	1 cut(s)	1700
XapI	RAATTY	6 cut(s)	212, 381, 410, 468, 763, 2181
XbaI	TCTAGA	1 cut(s)	83
XceI	RCATGY	2 cut(s)	546, 2037
XcmI	CCANNNNNNNNNTGG	1 cut(s)	431
XmnI	GAANNNNTTC	3 cut(s)	459, 1684, 1836
XspI	CTAG	6 cut(s)	84, 683, 714, 720, 1434, 1923
ZrmI	AGTACT	2 cut(s)	712, 923

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.