MD16G1259500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012165
Physical Location & Seq
Reverse (-)
31645871 .. 31651017
5147 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1259500.v1.1.491

Sequence Viewer

Length: 1647 bp
ATGGTGCAGTTGATGAAATCCGGAGATCTCCGACCAGAACTCCATACAATGCCGTGTACAGAAAAGGTACCGGTGAAGTTGGAGATTGAGGATTCTTTAGAAGAGGAGCATGGTCCGCTCAACAAACGTCCTAAACCCTCTCCTGCTTTTCAAGAGAGGAGTGTTGGGGATAATGCGTTTGCTGTTCAACCGTCACAATACTATCCACTTGATGAGCCTAGCCCTTTAGGTTTGAGGCTGAGAAAGAGCCCATCCTTATTAGAGTTAATTCAAATGAAGCTTGCTCATGGGAGTGCTTCTGCAGCTGGAGCGACAGAGAGCGAAAATCTTAACTCTGGAGTTAAAACGGAATGTAAAGCAAATGCTCCATTAGGGATGGTGGATAAGCTGAAGGCTTCGAATTTTCCAGCTTCACTTTTAAGTATTGGGGGTTGGGAGTATAAATCAAGATATGAGGGAGATTTAGTGGCGAAGTGTTACTTTGCTAAGCATAAGCTTGTTTGGGAAGTTCTCGAAGGTGGTCTCAAGAGTAAAATAGAAATCCAGTGGTCAGATATTATGGCTCTGAAAGCAAATTGTCCTGATGATGGACCTGGTACTTTTACTGTTGTGTTGGCTAGACAACCCCTTTTCTTCCGGGAGACTAACCCTCAACCTAGAAAGCACACATTGTGGCAGGCAACAGCAGATTTTACTGGTGGACAGGCTAGCAGTTATAGGGAACATTTTCTGCAATGTCCTCAAGGATTATTAAACAAACATTTTGAAAAGCTTATCCAGTGTGACACACGACTTTGTGTCTTAAGCCGGCAGCCAGGGATTGTTTTGGAATCACCATATTTTGAACCACGCTCTTCTGCTTTTGAAGATCCAGGCGAATGCAAAGGTCATGGTTTTGGTCAACTGGGGAATGGACCAAATGGTAAAGGTTCGTCTGTTGCTGGTTTTCAAGATATAGCATCACCATCTGCAAGCCAGTCATCTTCTTTGAATATTGACCAGCAGGATACAACTTCTATGGCATTCGAACATCAGTCCAGAGAAGCGCCTTCCCCCAGCTCAGTTATGGATGCTCATGCAATGGAAGGAAATGGCAGTTCGGAAGCTGTTGATTCCAGGGAGGACAGAAATTGGGAGCAAATAAAAGTACCTGGGCTTCACTCATCAATGTCGATGAGTGATCTTATGAGTCACATTGGAAACTGTATTTCAGAACAAATGACTTCTGGAAATCCGCCTTCTGCCGATCAGCAGTCAGAATACCAGGACATGCTAGAGGACATTGCACAATACCTGCTGAGCGACACTCAATTTACAACAGCTTCGGATGAAAAATCGCTCATGTCAAGGGTCGATTCTCTTTGTTGCCTTCTGCAGAAGGAACCCGCTGCAGCTCATACAAAGGTTGATGGTGATAGTTACGTTGAAGGATCAGGTAGTGGAAGGGATGTTCAGCCCAATCACACTCCTGATAATAAATCTGGAGCTGTTATCAAGGACCCTGAAGACGGTGTTTCTGACGGGAAGAAGGCACCAGGAATGTCAAGAAAAGACTCCTTTGTGGAATTGCTGTTTCATCTGCCTCGGATTGCCTCGCTTCCCTCACTTCCGAAAGTCTTGTTTAACATTTCCAAAGAAGATTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

549

Amino Acids

59.81

Weight (kDa)

5.26

Isoelectric Point (pI)

55.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_TRF2_HOY1 PF24818 134 - 252 1.4e-59 TRF2/HOY1 PH-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24100 AT4G30780
fragaria_vesca FvH4_4g10180
malus_domestica MD00G1164000.v1.1 MD16G1259500.v1.1
prunus_persica Prupe.1G102300_v2.0.a1
pyrus_communis pycom13g28620 pycom16g22740
rosa_chinensis RchiOBHm_Chr4g0405561
rosa_laevigata RLG00000008836
rosa_multiflora Rmu_sc0005023.1_g000013
rosa_roxburghii Rroxscaffold_5G00350070
rosa_rugosa Rorug04G0060600 Rorug04G0060600
rosa_samantha Rh4AG132500 Rh4BG127400 Rh4CG140200 Rh4DG126800
rosa_wichuraiana Rw4G010730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1302
Acc65I GGTACC 1 cut(s) 67
AccB1I GGYRCC 2 cut(s) 67, 1531
AccBSI CCGCTC 1 cut(s) 118
AccIII TCCGGA 1 cut(s) 20
AciI CCGC 3 cut(s) 116, 1235, 1386
AclWI GGATC 2 cut(s) 863, 1438
AcsI RAATTY 1 cut(s) 400
AcuI CTGAAG 2 cut(s) 410, 1524
AdeI CACNNNGTG 1 cut(s) 672
AfaI GTAC 4 cut(s) 58, 69, 598, 1149
AfiI CCNNNNNNNGG 2 cut(s) 587, 1508
AflII CTTAAG 1 cut(s) 802
AgeI ACCGGT 1 cut(s) 70
AgsI TTSAA 9 cut(s) 152, 188, 272, 767, 845, 866, 950, 991, 1427
AhdI GACNNNNNGTC 1 cut(s) 797
AjnI CCWGG 7 cut(s) 592, 814, 871, 1115, 1150, 1263, 1534
AloI GAACNNNNNNTCC 2 cut(s) 1434, 1466
Alw26I GTCTC 2 cut(s) 527, 635
AlwI GGATC 2 cut(s) 863, 1438
Aor13HI TCCGGA 1 cut(s) 20
ApeKI GCWGC 4 cut(s) 302, 811, 1388, 1391
ApoI RAATTY 1 cut(s) 400
AsiGI ACCGGT 1 cut(s) 70
Asp700I GAANNNNTTC 1 cut(s) 726
Asp718I GGTACC 1 cut(s) 67
AspLEI GCGC 1 cut(s) 1048
AspS9I GGNCC 4 cut(s) 113, 590, 914, 1498
AsuC2I CCSGG 1 cut(s) 638
AsuHPI GGTGA 4 cut(s) 85, 825, 954, 1424
AsuII TTCGAA 2 cut(s) 398, 1026
AsuNHI GCTAGC 1 cut(s) 707
AvaII GGWCC 4 cut(s) 113, 590, 914, 1498
BanI GGYRCC 2 cut(s) 67, 1531
BanII GRGCYC 1 cut(s) 251
BarI GAAGNNNNNNTAC 2 cut(s) 1140, 1172
BbsI GAAGAC 1 cut(s) 1512
BbvI GCAGC 4 cut(s) 314, 823, 1375, 1403
BccI CCATC 5 cut(s) 259, 370, 581, 973, 1403
BceAI ACGGC 1 cut(s) 37
BciT130I CCWGG 7 cut(s) 594, 816, 873, 1117, 1152, 1265, 1536
BciVI GTATCC 1 cut(s) 1000
BcnI CCSGG 1 cut(s) 638
BcoDI GTCTC 2 cut(s) 527, 635
BfaI CTAG 6 cut(s) 219, 618, 657, 708, 1274, 1645
BfmI CTRYAG 3 cut(s) 300, 1373, 1389
BfoI RGCGCY 1 cut(s) 1049
BfrI CTTAAG 1 cut(s) 802
BfuAI ACCTGC 1 cut(s) 1302
BfuI GTATCC 1 cut(s) 1000
BglII AGATCT 1 cut(s) 25
BisI GCNGC 4 cut(s) 303, 812, 1389, 1392
BlpI GCTNAGC 2 cut(s) 486, 1298
BlsI GCNGC 4 cut(s) 304, 813, 1390, 1393
Bme1390I CCNGG 8 cut(s) 594, 638, 816, 873, 1117, 1152, 1265, 1536
Bme18I GGWCC 4 cut(s) 113, 590, 914, 1498
BmeRI GACNNNNNGTC 1 cut(s) 797
BmgT120I GGNCC 4 cut(s) 113, 590, 914, 1498
BmiI GGNNCC 4 cut(s) 69, 1383, 1500, 1533
BmrFI CCNGG 8 cut(s) 594, 638, 816, 873, 1117, 1152, 1265, 1536
BmrI ACTGGG 1 cut(s) 914
BmsI GCATC 2 cut(s) 968, 1060
BmtI GCTAGC 1 cut(s) 711
BmuI ACTGGG 1 cut(s) 914
BpiI GAAGAC 1 cut(s) 1512
BplI GAGNNNNNCTC 2 cut(s) 1291, 1323
BpmI CTGGAG 3 cut(s) 327, 357, 1503
Bpu1102I GCTNAGC 2 cut(s) 486, 1298
Bpu14I TTCGAA 2 cut(s) 398, 1026
BpuEI CTTGAG 2 cut(s) 509, 726
BpuMI CCSGG 1 cut(s) 638
BsaI GGTCTC 1 cut(s) 527
BsaJI CCNNGG 4 cut(s) 815, 1116, 1151, 1583
BsaWI WCCGGW 2 cut(s) 20, 70
BsaXI ACNNNNNCTCC 4 cut(s) 24, 54, 450, 480
Bsc4I CCNNNNNNNGG 2 cut(s) 587, 1508
Bse118I RCCGGY 2 cut(s) 70, 807
Bse1I ACTGG 5 cut(s) 544, 700, 778, 909, 976
Bse3DI GCAATG 3 cut(s) 740, 1086, 1281
BseAI TCCGGA 1 cut(s) 20
BseBI CCWGG 7 cut(s) 594, 816, 873, 1117, 1152, 1265, 1536
BseDI CCNNGG 4 cut(s) 815, 1116, 1151, 1583
BseGI GGATG 5 cut(s) 251, 381, 1075, 1333, 1453
BseLI CCNNNNNNNGG 2 cut(s) 587, 1508
BseMI GCAATG 3 cut(s) 740, 1086, 1281
BseMII CTCAG 3 cut(s) 230, 1074, 1289
BseNI ACTGG 5 cut(s) 544, 700, 778, 909, 976
BseRI GAGGAG 2 cut(s) 119, 172
BseXI GCAGC 4 cut(s) 314, 823, 1375, 1403
BseYI CCCAGC 1 cut(s) 1055
BsgI GTGCAG 1 cut(s) 26
BshNI GGYRCC 2 cut(s) 67, 1531
BshTI ACCGGT 1 cut(s) 70
BsiSI CCGG 4 cut(s) 21, 71, 637, 808
BslI CCNNNNNNNGG 2 cut(s) 587, 1508
BsmAI GTCTC 2 cut(s) 527, 635
BsmI GAATGC 2 cut(s) 884, 1022
Bso31I GGTCTC 1 cut(s) 527
Bsp119I TTCGAA 2 cut(s) 398, 1026
Bsp1286I GDGCHC 1 cut(s) 251
Bsp13I TCCGGA 1 cut(s) 20
Bsp1407I TGTACA 1 cut(s) 56
Bsp143I GATC 5 cut(s) 25, 868, 1180, 1246, 1430
Bsp1720I GCTNAGC 2 cut(s) 486, 1298
BspACI CCGC 3 cut(s) 116, 1235, 1386
BspCNI CTCAG 3 cut(s) 231, 1073, 1290
BspEI TCCGGA 1 cut(s) 20
BspLI GGNNCC 4 cut(s) 69, 1383, 1500, 1533
BspMAI CTGCAG 3 cut(s) 304, 1377, 1393
BspMI ACCTGC 1 cut(s) 1302
BspOI GCTAGC 1 cut(s) 711
BspPI GGATC 2 cut(s) 863, 1438
BspQI GCTCTTC 1 cut(s) 859
BspT104I TTCGAA 2 cut(s) 398, 1026
BspT107I GGYRCC 2 cut(s) 67, 1531
BspTI CTTAAG 1 cut(s) 802
BspTNI GGTCTC 1 cut(s) 527
BsrBI CCGCTC 1 cut(s) 118
BsrDI GCAATG 3 cut(s) 740, 1086, 1281
BsrFI RCCGGY 2 cut(s) 70, 807
BsrGI TGTACA 1 cut(s) 56
BsrI ACTGG 5 cut(s) 544, 700, 778, 909, 976
BssAI RCCGGY 2 cut(s) 70, 807
BssECI CCNNGG 4 cut(s) 815, 1116, 1151, 1583
BssMI GATC 5 cut(s) 25, 868, 1180, 1246, 1430
Bst2UI CCWGG 7 cut(s) 594, 816, 873, 1117, 1152, 1265, 1536
Bst4CI ACNGT 4 cut(s) 192, 607, 1205, 1511
Bst6I CTCTTC 2 cut(s) 96, 859
BstAFI CTTAAG 1 cut(s) 802
BstAUI TGTACA 1 cut(s) 56
BstBI TTCGAA 2 cut(s) 398, 1026
BstC8I GCNNGC 5 cut(s) 282, 678, 709, 809, 973
BstDEI CTNAG 4 cut(s) 239, 486, 1060, 1298
BstF5I GGATG 5 cut(s) 251, 381, 1075, 1333, 1453
BstH2I RGCGCY 1 cut(s) 1049
BstHHI GCGC 1 cut(s) 1048
BstKTI GATC 5 cut(s) 28, 871, 1183, 1249, 1433
BstMAI GTCTC 2 cut(s) 527, 635
BstMBI GATC 5 cut(s) 25, 868, 1180, 1246, 1430
BstMWI GCNNNNNNNGC 4 cut(s) 115, 302, 308, 569
BstNI CCWGG 7 cut(s) 594, 816, 873, 1117, 1152, 1265, 1536
BstNSI RCATGY 1 cut(s) 1273
BstSCI CCNGG 8 cut(s) 592, 636, 814, 871, 1115, 1150, 1263, 1534
BstSFI CTRYAG 3 cut(s) 300, 1373, 1389
BstV1I GCAGC 4 cut(s) 314, 823, 1375, 1403
BstV2I GAAGAC 1 cut(s) 1512
BstX2I RGATCY 2 cut(s) 25, 868
BstYI RGATCY 2 cut(s) 25, 868
BsuI GTATCC 1 cut(s) 1000
BtsCI GGATG 5 cut(s) 251, 381, 1075, 1333, 1453
BtsIMutI CAGTG 2 cut(s) 551, 785
BveI ACCTGC 1 cut(s) 1302
Cac8I GCNNGC 5 cut(s) 282, 678, 709, 809, 973
CfoI GCGC 1 cut(s) 1048
Cfr10I RCCGGY 2 cut(s) 70, 807
Cfr13I GGNCC 4 cut(s) 113, 590, 914, 1498
CsiI ACCWGGT 1 cut(s) 592
Csp6I GTAC 4 cut(s) 57, 68, 597, 1148
CspAI ACCGGT 1 cut(s) 70
CviAII CATG 6 cut(s) 110, 287, 890, 1076, 1270, 1342
CviQI GTAC 4 cut(s) 57, 68, 597, 1148
DdeI CTNAG 4 cut(s) 239, 486, 1060, 1298
DpnI GATC 5 cut(s) 27, 870, 1182, 1248, 1432
DpnII GATC 5 cut(s) 25, 868, 1180, 1246, 1430
DraIII CACNNNGTG 1 cut(s) 672
DriI GACNNNNNGTC 1 cut(s) 797
Eam1104I CTCTTC 2 cut(s) 96, 859
Eam1105I GACNNNNNGTC 1 cut(s) 797
EarI CTCTTC 2 cut(s) 96, 859
EciI GGCGGA 1 cut(s) 1224
Eco24I GRGCYC 1 cut(s) 251
Eco31I GGTCTC 1 cut(s) 527
Eco47I GGWCC 4 cut(s) 113, 590, 914, 1498
Eco57I CTGAAG 2 cut(s) 410, 1524
EcoO109I RGGNCCY 1 cut(s) 1498
EcoRII CCWGG 7 cut(s) 592, 814, 871, 1115, 1150, 1263, 1534
EcoT38I GRGCYC 1 cut(s) 251
FaeI CATG 6 cut(s) 113, 290, 893, 1079, 1273, 1345
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FatI CATG 6 cut(s) 109, 286, 889, 1075, 1269, 1341
FauI CCCGC 1 cut(s) 1393
Fnu4HI GCNGC 4 cut(s) 303, 812, 1389, 1392
FokI GGATG 5 cut(s) 238, 388, 1082, 1340, 1460
FriOI GRGCYC 1 cut(s) 251
Fsp4HI GCNGC 4 cut(s) 303, 812, 1389, 1392
FspBI CTAG 6 cut(s) 219, 618, 657, 708, 1274, 1645
GlaI GCGC 1 cut(s) 1047
GluI GCNGC 4 cut(s) 303, 812, 1389, 1392
GsaI CCCAGC 1 cut(s) 1059
GsuI CTGGAG 3 cut(s) 327, 357, 1503
HaeII RGCGCY 1 cut(s) 1049
HapII CCGG 4 cut(s) 21, 71, 637, 808
HhaI GCGC 1 cut(s) 1048
Hin1II CATG 6 cut(s) 113, 290, 893, 1079, 1273, 1345
Hin6I GCGC 1 cut(s) 1046
HinP1I GCGC 1 cut(s) 1046
HincII GTYRAC 1 cut(s) 902
HindII GTYRAC 1 cut(s) 902
HindIII AAGCTT 3 cut(s) 278, 494, 770
HinfI GANTC 6 cut(s) 92, 830, 1112, 1189, 1355, 1553
HpaII CCGG 4 cut(s) 21, 71, 637, 808
HphI GGTGA 4 cut(s) 85, 825, 954, 1424
Hpy166II GTNNAC 3 cut(s) 57, 701, 902
Hpy8I GTNNAC 3 cut(s) 57, 701, 902
HpyCH4III ACNGT 4 cut(s) 192, 607, 1205, 1511
HpyCH4IV ACGT 2 cut(s) 127, 1422
HpyCH4V TGCA 9 cut(s) 7, 302, 733, 882, 971, 1079, 1286, 1375, 1391
HpyF10VI GCNNNNNNNGC 4 cut(s) 115, 302, 308, 569
HpyF3I CTNAG 4 cut(s) 239, 486, 1060, 1298
HpySE526I ACGT 2 cut(s) 127, 1422
Hsp92II CATG 6 cut(s) 113, 290, 893, 1079, 1273, 1345
HspAI GCGC 1 cut(s) 1046
Kpn2I TCCGGA 1 cut(s) 20
KpnI GGTACC 1 cut(s) 71
KroI GCCGGC 1 cut(s) 807
KroNI GCCGGC 1 cut(s) 809
Kzo9I GATC 5 cut(s) 25, 868, 1180, 1246, 1430
LguI GCTCTTC 1 cut(s) 859
LmnI GCTCC 5 cut(s) 106, 308, 370, 1135, 1484
Lsp1109I GCAGC 4 cut(s) 314, 823, 1375, 1403
LweI GCATC 2 cut(s) 968, 1060
MabI ACCWGGT 1 cut(s) 592
MaeI CTAG 6 cut(s) 219, 618, 657, 708, 1274, 1645
MaeII ACGT 2 cut(s) 127, 1422
MaeIII GTNAC 5 cut(s) 192, 476, 782, 1190, 1418
MalI GATC 5 cut(s) 27, 870, 1182, 1248, 1432
MbiI CCGCTC 1 cut(s) 118
MboI GATC 5 cut(s) 25, 868, 1180, 1246, 1430
MboII GAAGA 7 cut(s) 113, 625, 846, 878, 975, 1517, 1537
MflI RGATCY 2 cut(s) 25, 868
MhlI GDGCHC 1 cut(s) 251
MluCI AATT 6 cut(s) 267, 400, 574, 1129, 1310, 1565
MlyI GAGTC 2 cut(s) 1198, 1547
MmeI TCCRAC 2 cut(s) 55, 60
MroI TCCGGA 1 cut(s) 20
MroNI GCCGGC 1 cut(s) 807
MroXI GAANNNNTTC 1 cut(s) 726
MseI TTAA 7 cut(s) 266, 330, 342, 419, 752, 803, 1623
MslI CAYNNNNRTG 1 cut(s) 291
MspA1I CMGCKG 2 cut(s) 305, 1388
MspCI CTTAAG 1 cut(s) 802
MspI CCGG 4 cut(s) 21, 71, 637, 808
MspR9I CCNGG 8 cut(s) 594, 638, 816, 873, 1117, 1152, 1265, 1536
Mva1269I GAATGC 2 cut(s) 884, 1022
MvaI CCWGG 7 cut(s) 594, 816, 873, 1117, 1152, 1265, 1536
MwoI GCNNNNNNNGC 4 cut(s) 115, 302, 308, 569
NaeI GCCGGC 1 cut(s) 809
NciI CCSGG 1 cut(s) 638
NdeII GATC 5 cut(s) 25, 868, 1180, 1246, 1430
NgoMIV GCCGGC 1 cut(s) 807
NheI GCTAGC 1 cut(s) 707
NlaIII CATG 6 cut(s) 113, 290, 893, 1079, 1273, 1345
NlaIV GGNNCC 4 cut(s) 69, 1383, 1500, 1533
NmuCI GTSAC 3 cut(s) 192, 782, 1190
NspI RCATGY 1 cut(s) 1273
NspV TTCGAA 2 cut(s) 398, 1026
PciSI GCTCTTC 1 cut(s) 859
PctI GAATGC 2 cut(s) 884, 1022
PdiI GCCGGC 1 cut(s) 809
PdmI GAANNNNTTC 1 cut(s) 726
PfeI GAWTC 4 cut(s) 92, 830, 1112, 1355
PfoI TCCNGGA 1 cut(s) 636
PinAI ACCGGT 1 cut(s) 70
PkrI GCNGC 4 cut(s) 304, 813, 1390, 1393
PleI GAGTC 2 cut(s) 1197, 1547
PpsI GAGTC 2 cut(s) 1197, 1547
PpuMI RGGWCCY 1 cut(s) 1498
Psp5II RGGWCCY 1 cut(s) 1498
Psp6I CCWGG 7 cut(s) 592, 814, 871, 1115, 1150, 1263, 1534
PspFI CCCAGC 1 cut(s) 1055
PspGI CCWGG 7 cut(s) 592, 814, 871, 1115, 1150, 1263, 1534
PspN4I GGNNCC 4 cut(s) 69, 1383, 1500, 1533
PspPI GGNCC 4 cut(s) 113, 590, 914, 1498
PspPPI RGGWCCY 1 cut(s) 1498
PstI CTGCAG 3 cut(s) 304, 1377, 1393
PsuI RGATCY 2 cut(s) 25, 868
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 4 cut(s) 58, 69, 598, 1149
RsaNI GTAC 4 cut(s) 57, 68, 597, 1148
RseI CAYNNNNRTG 1 cut(s) 291
SapI GCTCTTC 1 cut(s) 859
SaqAI TTAA 7 cut(s) 266, 330, 342, 419, 752, 803, 1623
SatI GCNGC 4 cut(s) 303, 812, 1389, 1392
Sau3AI GATC 5 cut(s) 25, 868, 1180, 1246, 1430
Sau96I GGNCC 4 cut(s) 113, 590, 914, 1498
SchI GAGTC 2 cut(s) 1198, 1547
ScrFI CCNGG 8 cut(s) 594, 638, 816, 873, 1117, 1152, 1265, 1536
SduI GDGCHC 1 cut(s) 251
SexAI ACCWGGT 1 cut(s) 592
SfaNI GCATC 2 cut(s) 968, 1060
SfcI CTRYAG 3 cut(s) 300, 1373, 1389
SfuI TTCGAA 2 cut(s) 398, 1026
SinI GGWCC 4 cut(s) 113, 590, 914, 1498
SmiMI CAYNNNNRTG 1 cut(s) 291
SmlI CTYRAG 3 cut(s) 524, 741, 802
SmoI CTYRAG 3 cut(s) 524, 741, 802
Sse9I AATT 6 cut(s) 267, 400, 574, 1129, 1310, 1565
SsiI CCGC 3 cut(s) 116, 1235, 1386
SspI AATATT 1 cut(s) 994
SspMI CTAG 6 cut(s) 219, 618, 657, 708, 1274, 1645
StyD4I CCNGG 8 cut(s) 592, 636, 814, 871, 1115, 1150, 1263, 1534
TaaI ACNGT 4 cut(s) 192, 607, 1205, 1511
TaiI ACGT 2 cut(s) 130, 1425
TaqI TCGA 5 cut(s) 398, 513, 1026, 1172, 1353
TasI AATT 6 cut(s) 267, 400, 574, 1129, 1310, 1565
TatI WGTACW 1 cut(s) 56
TfiI GAWTC 4 cut(s) 92, 830, 1112, 1355
Tru1I TTAA 7 cut(s) 266, 330, 342, 419, 752, 803, 1623
Tru9I TTAA 7 cut(s) 266, 330, 342, 419, 752, 803, 1623
TscAI CASTG 2 cut(s) 551, 785
TseFI GTSAC 3 cut(s) 192, 782, 1190
TseI GCWGC 4 cut(s) 302, 811, 1388, 1391
Tsp45I GTSAC 3 cut(s) 192, 782, 1190
TspDTI ATGAA 4 cut(s) 29, 290, 1344, 1565
TspGWI ACGGA 1 cut(s) 362
TspRI CASTG 2 cut(s) 551, 785
Vha464I CTTAAG 1 cut(s) 802
VpaK11BI GGWCC 4 cut(s) 113, 590, 914, 1498
XapI RAATTY 1 cut(s) 400
XceI RCATGY 1 cut(s) 1273
XcmI CCANNNNNNNNNTGG 1 cut(s) 1063
XmnI GAANNNNTTC 1 cut(s) 726
XspI CTAG 6 cut(s) 219, 618, 657, 708, 1274, 1645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.