Rh4CG140200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0012165
Physical Location & Seq
Forward (+)
27802943 .. 27809120
6178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG140200.1

Sequence Viewer

Length: 1359 bp
ATGAAGCTTGCTCAGGGGGGTCCTCCTGCAGCGGGAGATGTACCAAGAGAAAATCTTAACTCTGGAGTTAAAAAGGAGATTAAAGCAAATGCTGCGGTGGGGGCTACTGATAAGCTTAAAGCATCAAATTTTCCAGCTTCAATGTTAAGGATCGGTAGCTGGGAGTACAAATCAAGGTACGAGGGTGATTTAGTGGCAAAGTGTTATTTTGCAAAGCATAAGCTCGTATGGGAGGTTCTTGAAGGAGGCCTCAAGAGCAAAATAGAAATCCAGTGGTCAGATATCATGGCCCTGAAAGCAAACTGTCCTGATGATGGACCTGGTACTTTGAATGTTGTGTTGGCTAGACAACCTCTGTTCTTCAGGGAGACTAATCCTCAGCCTAGAAAGCATACTTTATGGCAAGCAACCTCAGATTTTACTGATGGGCAAGCAAGCCTGCATAGGCAACATTTTCTGCAATGTCCACAAGGACTGTTAAACAAGCATTTTGAAAAGCTTATTCAGTGTGACACACGTCTTTGCTTCTTAAGTCGACAGCCAGAGATCGTTTTGGATTCACCTTATTTTGAACTGCGGACTTCTGTATTTGAAGACCAAGGAGAATCCAAAGGCCTTGGTTTTGACCAACCGGCAAGTGGTAAAGGATCCTCCATTTCTGGTTTCCAGGATATAGCATCACCATCTGCAAGTCAGTCATCATCTTTGAAGGCAGACCAGCAGGATTCAGCTGGTATGACATTAGAGCATCGGTCCAGAGAAGCTCCTTCCCCTAGCTCAGGAAATGGAAGTTCTGAAGTTGTTGATTCCAGGGGGCCAAGAAACTGGGAACAGATAAAAGTGCCTGGGCTCCATCCTTCAATGTCGATGAGTGATTTTATGAGTCACATTGGGAACTGTATTTCAGAACAAATGACTTCCGGAAATCCACCTTCTGCCGATCAACAGTCGGAATACCAGGATATTCTTGAGGACATTGCGCAGTACCTGTTAAGTGACACTCAATTAACTGCAGCAGATGAGAGTCATGAGACATCTCTCATGTCCAGGGTCAATTCTCTTTGTTGCCTTTTGCAGAAGGACCCCACTTCTCTTCAGACTTCGCAGGTTGAAGTTGAAGGCATAGATGGTGGAAGGGATATTCAGCTTAACCACACTCCTGGTGGTATATCTGGAGCTGATATGCAGGCTCCTGAAGAGGATATGAAGGATGTTCCTGGAGGAAAGAAGGCACCGGGAATGTCGAGAAAAGACTCCTTCGGGGAATTGCTGTTTCACCTCCCTCGCATCGCCTCTCTTCCCTCACTTCCAAAAGTCTTGTTCAACATTTCAGAAGATTGTGAGAGTCAAGGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

452

Amino Acids

49.33

Weight (kDa)

5.32

Isoelectric Point (pI)

50.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_TRF2_HOY1 PF24818 43 - 161 6.4e-62 TRF2/HOY1 PH-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24100 AT4G30780
fragaria_vesca FvH4_4g10180
malus_domestica MD00G1164000.v1.1 MD16G1259500.v1.1
prunus_persica Prupe.1G102300_v2.0.a1
pyrus_communis pycom13g28620 pycom16g22740
rosa_chinensis RchiOBHm_Chr4g0405561
rosa_laevigata RLG00000008836
rosa_multiflora Rmu_sc0005023.1_g000013
rosa_roxburghii Rroxscaffold_5G00350070
rosa_rugosa Rorug04G0060600 Rorug04G0060600
rosa_samantha Rh4AG132500 Rh4BG127400 Rh4CG140200 Rh4DG126800
rosa_wichuraiana Rw4G010730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 981
Acc36I ACCTGC 1 cut(s) 1096
AccB1I GGYRCC 1 cut(s) 1231
AccI GTMKAC 1 cut(s) 535
AccIII TCCGGA 1 cut(s) 920
AciI CCGC 3 cut(s) 32, 95, 577
AclWI GGATC 3 cut(s) 158, 642, 655
AcsI RAATTY 1 cut(s) 127
AcuI CTGAAG 4 cut(s) 346, 816, 1079, 1215
AfaI GTAC 5 cut(s) 42, 167, 179, 325, 986
AfiI CCNNNNNNNGG 4 cut(s) 32, 314, 638, 779
AflII CTTAAG 1 cut(s) 529
AflIII ACRYGT 1 cut(s) 515
AjiI CACGTC 1 cut(s) 518
AjnI CCWGG 8 cut(s) 319, 666, 809, 844, 957, 1046, 1159, 1216
AjuI GAANNNNNNNTTGG 2 cut(s) 323, 355
Alw26I GTCTC 2 cut(s) 362, 1025
AlwI GGATC 3 cut(s) 158, 642, 655
AlwNI CAGNNNCTG 1 cut(s) 988
Aor13HI TCCGGA 1 cut(s) 920
AoxI GGCC 4 cut(s) 247, 288, 613, 815
ApeKI GCWGC 3 cut(s) 29, 92, 1013
ApoI RAATTY 1 cut(s) 127
AspLEI GCGC 1 cut(s) 982
AspS9I GGNCC 6 cut(s) 20, 289, 317, 753, 815, 1081
AsuC2I CCSGG 1 cut(s) 1236
AsuHPI GGTGA 4 cut(s) 197, 552, 672, 1268
AvaII GGWCC 4 cut(s) 20, 317, 753, 1081
BaeI ACNNNNGTAYC 2 cut(s) 169, 202
BamHI GGATCC 1 cut(s) 647
BanI GGYRCC 1 cut(s) 1231
BanII GRGCYC 1 cut(s) 852
BbsI GAAGAC 1 cut(s) 600
BbvCI CCTCAGC 1 cut(s) 378
BbvI GCAGC 3 cut(s) 41, 79, 1025
BccI CCATC 5 cut(s) 308, 419, 691, 861, 1121
BciT130I CCWGG 8 cut(s) 321, 668, 811, 846, 959, 1048, 1161, 1218
BcnI CCSGG 1 cut(s) 1236
BcoDI GTCTC 2 cut(s) 362, 1025
BfaI CTAG 3 cut(s) 345, 384, 774
BfmI CTRYAG 2 cut(s) 27, 1011
BfrI CTTAAG 1 cut(s) 529
BfuAI ACCTGC 1 cut(s) 1096
BisI GCNGC 3 cut(s) 30, 93, 1014
BlsI GCNGC 3 cut(s) 31, 94, 1015
Bme1390I CCNGG 9 cut(s) 321, 668, 811, 846, 959, 1048, 1161, 1218, 1236
Bme18I GGWCC 4 cut(s) 20, 317, 753, 1081
BmgBI CACGTC 1 cut(s) 518
BmgT120I GGNCC 6 cut(s) 20, 289, 317, 753, 815, 1081
BmiI GGNNCC 7 cut(s) 21, 649, 816, 851, 1083, 1191, 1233
BmrFI CCNGG 9 cut(s) 321, 668, 811, 846, 959, 1048, 1161, 1218, 1236
BmrI ACTGGG 1 cut(s) 835
BmsI GCATC 4 cut(s) 131, 686, 757, 1296
BmuI ACTGGG 1 cut(s) 835
BoxI GACNNNNGTC 1 cut(s) 516
BpiI GAAGAC 1 cut(s) 600
BpmI CTGGAG 3 cut(s) 84, 1194, 1239
Bpu10I CCTNAGC 3 cut(s) 12, 378, 778
BpuEI CTTGAG 2 cut(s) 236, 989
BpuMI CCSGG 1 cut(s) 1236
BsaJI CCNNGG 5 cut(s) 598, 616, 810, 845, 1047
BsaWI WCCGGW 1 cut(s) 920
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 314, 638, 779
Bse118I RCCGGY 1 cut(s) 631
Bse1I ACTGG 2 cut(s) 271, 830
Bse3DI GCAATG 2 cut(s) 467, 975
BseAI TCCGGA 1 cut(s) 920
BseBI CCWGG 8 cut(s) 321, 668, 811, 846, 959, 1048, 1161, 1218
BseDI CCNNGG 5 cut(s) 598, 616, 810, 845, 1047
BseGI GGATG 2 cut(s) 853, 1216
BseLI CCNNNNNNNGG 4 cut(s) 32, 314, 638, 779
BseMI GCAATG 2 cut(s) 467, 975
BseMII CTCAG 4 cut(s) 26, 392, 426, 792
BseNI ACTGG 2 cut(s) 271, 830
BseXI GCAGC 3 cut(s) 41, 79, 1025
BseYI CCCAGC 1 cut(s) 159
BshFI GGCC 4 cut(s) 249, 290, 615, 817
BshNI GGYRCC 1 cut(s) 1231
BsiSI CCGG 3 cut(s) 632, 921, 1235
BslI CCNNNNNNNGG 4 cut(s) 32, 314, 638, 779
BsmAI GTCTC 2 cut(s) 362, 1025
BsnI GGCC 4 cut(s) 249, 290, 615, 817
Bsp1286I GDGCHC 1 cut(s) 852
Bsp13I TCCGGA 1 cut(s) 920
Bsp143I GATC 4 cut(s) 150, 546, 647, 940
BspACI CCGC 3 cut(s) 32, 95, 577
BspANI GGCC 4 cut(s) 249, 290, 615, 817
BspCNI CTCAG 4 cut(s) 25, 391, 425, 791
BspEI TCCGGA 1 cut(s) 920
BspHI TCATGA 1 cut(s) 1027
BspLI GGNNCC 7 cut(s) 21, 649, 816, 851, 1083, 1191, 1233
BspMAI CTGCAG 2 cut(s) 31, 1015
BspMI ACCTGC 1 cut(s) 1096
BspPI GGATC 3 cut(s) 158, 642, 655
BspT107I GGYRCC 1 cut(s) 1231
BspTI CTTAAG 1 cut(s) 529
BsrDI GCAATG 2 cut(s) 467, 975
BsrFI RCCGGY 1 cut(s) 631
BsrI ACTGG 2 cut(s) 271, 830
BssAI RCCGGY 1 cut(s) 631
BssECI CCNNGG 5 cut(s) 598, 616, 810, 845, 1047
BssMI GATC 4 cut(s) 150, 546, 647, 940
BssT1I CCWWGG 2 cut(s) 598, 616
Bst2UI CCWGG 8 cut(s) 321, 668, 811, 846, 959, 1048, 1161, 1218
Bst4CI ACNGT 4 cut(s) 305, 477, 899, 948
Bst6I CTCTTC 3 cut(s) 1098, 1191, 1302
BstAFI CTTAAG 1 cut(s) 529
BstAPI GCANNNNNTGC 1 cut(s) 92
BstC8I GCNNGC 6 cut(s) 9, 405, 432, 436, 440, 1188
BstDEI CTNAG 4 cut(s) 12, 378, 412, 778
BstENI CCTNNNNNAGG 1 cut(s) 777
BstF5I GGATG 2 cut(s) 853, 1216
BstHHI GCGC 1 cut(s) 982
BstKTI GATC 4 cut(s) 153, 549, 650, 943
BstMAI GTCTC 2 cut(s) 362, 1025
BstMBI GATC 4 cut(s) 150, 546, 647, 940
BstMWI GCNNNNNNNGC 5 cut(s) 92, 101, 255, 296, 388
BstNI CCWGG 8 cut(s) 321, 668, 811, 846, 959, 1048, 1161, 1218
BstPAI GACNNNNGTC 1 cut(s) 516
BstSCI CCNGG 9 cut(s) 319, 666, 809, 844, 957, 1046, 1159, 1216, 1234
BstSFI CTRYAG 2 cut(s) 27, 1011
BstV1I GCAGC 3 cut(s) 41, 79, 1025
BstV2I GAAGAC 1 cut(s) 600
BstX2I RGATCY 1 cut(s) 647
BstXI CCANNNNNNTGG 2 cut(s) 825, 1160
BstYI RGATCY 1 cut(s) 647
BsuRI GGCC 4 cut(s) 249, 290, 615, 817
BtgZI GCGATG 1 cut(s) 1273
BtrI CACGTC 1 cut(s) 518
BtsCI GGATG 2 cut(s) 853, 1216
BtsIMutI CAGTG 2 cut(s) 278, 512
BveI ACCTGC 1 cut(s) 1096
Cac8I GCNNGC 6 cut(s) 9, 405, 432, 436, 440, 1188
CaiI CAGNNNCTG 1 cut(s) 988
CciI TCATGA 1 cut(s) 1027
CfoI GCGC 1 cut(s) 982
Cfr10I RCCGGY 1 cut(s) 631
Cfr13I GGNCC 6 cut(s) 20, 289, 317, 753, 815, 1081
CsiI ACCWGGT 1 cut(s) 319
Csp6I GTAC 5 cut(s) 41, 166, 178, 324, 985
CviAII CATG 3 cut(s) 286, 1028, 1042
CviQI GTAC 5 cut(s) 41, 166, 178, 324, 985
DdeI CTNAG 4 cut(s) 12, 378, 412, 778
DpnI GATC 4 cut(s) 152, 548, 649, 942
DpnII GATC 4 cut(s) 150, 546, 647, 940
Eam1104I CTCTTC 3 cut(s) 1098, 1191, 1302
EarI CTCTTC 3 cut(s) 1098, 1191, 1302
Eco130I CCWWGG 2 cut(s) 598, 616
Eco147I AGGCCT 2 cut(s) 249, 615
Eco24I GRGCYC 1 cut(s) 852
Eco32I GATATC 1 cut(s) 283
Eco47I GGWCC 4 cut(s) 20, 317, 753, 1081
Eco57I CTGAAG 4 cut(s) 346, 816, 1079, 1215
EcoNI CCTNNNNNAGG 1 cut(s) 777
EcoO109I RGGNCCY 2 cut(s) 20, 1081
EcoRII CCWGG 8 cut(s) 319, 666, 809, 844, 957, 1046, 1159, 1216
EcoRV GATATC 1 cut(s) 283
EcoT14I CCWWGG 2 cut(s) 598, 616
EcoT38I GRGCYC 1 cut(s) 852
ErhI CCWWGG 2 cut(s) 598, 616
FaeI CATG 3 cut(s) 289, 1031, 1045
FatI CATG 3 cut(s) 285, 1027, 1041
FauI CCCGC 1 cut(s) 25
FblI GTMKAC 1 cut(s) 535
Fnu4HI GCNGC 3 cut(s) 30, 93, 1014
FokI GGATG 2 cut(s) 840, 1223
FriOI GRGCYC 1 cut(s) 852
Fsp4HI GCNGC 3 cut(s) 30, 93, 1014
FspBI CTAG 3 cut(s) 345, 384, 774
FspI TGCGCA 1 cut(s) 981
GlaI GCGC 1 cut(s) 981
GluI GCNGC 3 cut(s) 30, 93, 1014
GsaI CCCAGC 1 cut(s) 163
GsuI CTGGAG 3 cut(s) 84, 1194, 1239
HaeIII GGCC 4 cut(s) 249, 290, 615, 817
HapII CCGG 3 cut(s) 632, 921, 1235
HhaI GCGC 1 cut(s) 982
Hin1II CATG 3 cut(s) 289, 1031, 1045
Hin6I GCGC 1 cut(s) 980
HinP1I GCGC 1 cut(s) 980
HincII GTYRAC 1 cut(s) 536
HindII GTYRAC 1 cut(s) 536
HindIII AAGCTT 3 cut(s) 5, 113, 497
HinfI GANTC 8 cut(s) 557, 605, 725, 806, 883, 1024, 1253, 1345
HpaII CCGG 3 cut(s) 632, 921, 1235
HphI GGTGA 4 cut(s) 197, 552, 672, 1268
Hpy166II GTNNAC 2 cut(s) 467, 536
Hpy188I TCNGA 7 cut(s) 280, 415, 796, 907, 952, 1098, 1333
Hpy8I GTNNAC 2 cut(s) 467, 536
HpyCH4III ACNGT 4 cut(s) 305, 477, 899, 948
HpyCH4IV ACGT 1 cut(s) 517
HpyCH4V TGCA 8 cut(s) 29, 212, 442, 460, 689, 1013, 1075, 1186
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 101, 255, 296, 388
HpyF3I CTNAG 4 cut(s) 12, 378, 412, 778
HpySE526I ACGT 1 cut(s) 517
Hsp92II CATG 3 cut(s) 289, 1031, 1045
HspAI GCGC 1 cut(s) 980
Kpn2I TCCGGA 1 cut(s) 920
Kzo9I GATC 4 cut(s) 150, 546, 647, 940
LmnI GCTCC 4 cut(s) 769, 855, 1175, 1195
Lsp1109I GCAGC 3 cut(s) 41, 79, 1025
LweI GCATC 4 cut(s) 131, 686, 757, 1296
MabI ACCWGGT 1 cut(s) 319
MaeI CTAG 3 cut(s) 345, 384, 774
MaeII ACGT 1 cut(s) 517
MaeIII GTNAC 3 cut(s) 509, 884, 995
MalI GATC 4 cut(s) 152, 548, 649, 942
MboI GATC 4 cut(s) 150, 546, 647, 940
MboII GAAGA 6 cut(s) 352, 605, 1085, 1208, 1289, 1346
MflI RGATCY 1 cut(s) 647
MhlI GDGCHC 1 cut(s) 852
MluCI AATT 4 cut(s) 127, 1004, 1054, 1265
MlyI GAGTC 4 cut(s) 892, 1033, 1247, 1354
MmeI TCCRAC 1 cut(s) 930
MroI TCCGGA 1 cut(s) 920
MspA1I CMGCKG 2 cut(s) 32, 731
MspCI CTTAAG 1 cut(s) 529
MspI CCGG 3 cut(s) 632, 921, 1235
MspR9I CCNGG 9 cut(s) 321, 668, 811, 846, 959, 1048, 1161, 1218, 1236
MvaI CCWGG 8 cut(s) 321, 668, 811, 846, 959, 1048, 1161, 1218
MwoI GCNNNNNNNGC 5 cut(s) 92, 101, 255, 296, 388
NciI CCSGG 1 cut(s) 1236
NdeII GATC 4 cut(s) 150, 546, 647, 940
NlaIII CATG 3 cut(s) 289, 1031, 1045
NlaIV GGNNCC 7 cut(s) 21, 649, 816, 851, 1083, 1191, 1233
NmuCI GTSAC 3 cut(s) 509, 884, 995
NsbI TGCGCA 1 cut(s) 981
PagI TCATGA 1 cut(s) 1027
PceI AGGCCT 2 cut(s) 249, 615
PfeI GAWTC 4 cut(s) 557, 605, 725, 806
PfoI TCCNGGA 2 cut(s) 666, 1216
PkrI GCNGC 3 cut(s) 31, 94, 1015
PleI GAGTC 4 cut(s) 891, 1032, 1247, 1353
PpsI GAGTC 4 cut(s) 891, 1032, 1247, 1353
PpuMI RGGWCCY 2 cut(s) 20, 1081
PshAI GACNNNNGTC 1 cut(s) 516
Psp5II RGGWCCY 2 cut(s) 20, 1081
Psp6I CCWGG 8 cut(s) 319, 666, 809, 844, 957, 1046, 1159, 1216
PspFI CCCAGC 1 cut(s) 159
PspGI CCWGG 8 cut(s) 319, 666, 809, 844, 957, 1046, 1159, 1216
PspN4I GGNNCC 7 cut(s) 21, 649, 816, 851, 1083, 1191, 1233
PspPI GGNCC 6 cut(s) 20, 289, 317, 753, 815, 1081
PspPPI RGGWCCY 2 cut(s) 20, 1081
PsrI GAACNNNNNNTAC 2 cut(s) 219, 251
PstI CTGCAG 2 cut(s) 31, 1015
PstNI CAGNNNCTG 1 cut(s) 988
PsuI RGATCY 1 cut(s) 647
PvuII CAGCTG 1 cut(s) 731
RsaI GTAC 5 cut(s) 42, 167, 179, 325, 986
RsaNI GTAC 5 cut(s) 41, 166, 178, 324, 985
SalI GTCGAC 1 cut(s) 534
SatI GCNGC 3 cut(s) 30, 93, 1014
Sau3AI GATC 4 cut(s) 150, 546, 647, 940
Sau96I GGNCC 6 cut(s) 20, 289, 317, 753, 815, 1081
SchI GAGTC 4 cut(s) 892, 1033, 1247, 1354
ScrFI CCNGG 9 cut(s) 321, 668, 811, 846, 959, 1048, 1161, 1218, 1236
SduI GDGCHC 1 cut(s) 852
SexAI ACCWGGT 1 cut(s) 319
SfaNI GCATC 4 cut(s) 131, 686, 757, 1296
SfcI CTRYAG 2 cut(s) 27, 1011
SinI GGWCC 4 cut(s) 20, 317, 753, 1081
SmlI CTYRAG 3 cut(s) 251, 529, 968
SmoI CTYRAG 3 cut(s) 251, 529, 968
Sse9I AATT 4 cut(s) 127, 1004, 1054, 1265
SseBI AGGCCT 2 cut(s) 249, 615
SsiI CCGC 3 cut(s) 32, 95, 577
SspMI CTAG 3 cut(s) 345, 384, 774
StuI AGGCCT 2 cut(s) 249, 615
StyD4I CCNGG 9 cut(s) 319, 666, 809, 844, 957, 1046, 1159, 1216, 1234
StyI CCWWGG 2 cut(s) 598, 616
TaaI ACNGT 4 cut(s) 305, 477, 899, 948
TaiI ACGT 1 cut(s) 520
TaqI TCGA 3 cut(s) 535, 866, 1244
TaqII GACCGA 1 cut(s) 741
TasI AATT 4 cut(s) 127, 1004, 1054, 1265
TatI WGTACW 1 cut(s) 165
TfiI GAWTC 4 cut(s) 557, 605, 725, 806
TscAI CASTG 2 cut(s) 278, 512
TseFI GTSAC 3 cut(s) 509, 884, 995
TseI GCWGC 3 cut(s) 29, 92, 1013
Tsp45I GTSAC 3 cut(s) 509, 884, 995
TspDTI ATGAA 2 cut(s) 17, 1220
TspRI CASTG 2 cut(s) 278, 512
Vha464I CTTAAG 1 cut(s) 529
VpaK11BI GGWCC 4 cut(s) 20, 317, 753, 1081
XagI CCTNNNNNAGG 1 cut(s) 777
XapI RAATTY 1 cut(s) 127
XcmI CCANNNNNNNNNTGG 2 cut(s) 635, 1160
XmiI GTMKAC 1 cut(s) 535
XspI CTAG 3 cut(s) 345, 384, 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.