Rh4AG132500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0012165
Physical Location & Seq
Forward (+)
28856535 .. 28862714
6180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG132500.1

Sequence Viewer

Length: 1620 bp
ATGGTGCAGTTGATGAAATCCGGCGATCTCCGCCAAACGACGCCGTGTAAGGATAAGCTGCCGGTGAAATTGGAGATCGAAGACTCCTTGGAGGAGGAGCATGGCCCCCTCAACAAGCGCTCCAAACCCTCTCCTGCTTTTCATGAGAGTTCGGGTGATAATGGGTTTGCTGTCCCAGCTCCCCAATACAATCCACTTGATGAGCCTAGCCCTTTGGGTTTGAGGCTCAGGAAGAGCCCGTCTTTATTGGAATTAATTCAAATGAAGCTTGCTCAGGGGGGTCCTCCTGCAGCGGGAGATGTACCAAGAGAAAATCTTAACTCTGGAGTTAAAAAGGAGATTAAAGCAAATGCTGCGGTGGGGGCTACTGATAAGCTTAAAGCATCAAATTTTCCAGCTTCAATGTTAAGGATCGGTAGCTGGGAGTACAAATCAAGGTACGAGGGTGATTTAGTGGCAAAGTGTTATTTTGCAAAGCATAAGCTCGTATGGGAGGTTCTTGAAGGAGGCCTCAAGAGCAAAATAGAAATCCAGTGGTCAGATATCATGGCCCTGAAAGCAAACTGTCCTGATGATGGACCTGGTACTTTGAATGTTGTGTTGGCTAGACAACCTCTGTTCTTCAGGGAGACTAATCCTCAGCCTAGAAAGCATACTTTATGGCAAGCAACCTCAGATTTTACTGATGGGCAAGCAAGCCTGCATAGGCAACATTTTCTGCAATGTCCACAAGGACTGTTAAACAAGCATTTTGAAAAGCTTATTCAGTGTGACACACGTCTTTGCTTCTTAAGTCGACAGCCAGAGATCGTTTTGGATTCACCTTATTTTGAACTGCGGACTTCTGTATTTGAAGACCAAGGAGAATCCAAAGGCCTTGGTTTTGACCAACCGGCAAGTGGTAAAGGATCCTCCATTTCTGGTTTCCAGGATATAGCATCACCATCTGCAAGTCAGTCATCATCTTTGAAGGCAGACCAGCAGGATTCAGCTGGTATGACATTAGAGCATCGGTCCAGAGAAGCTCCTTCCCCTAGCTCAGGAAATGGAAGTTCTGAAGTTGTTGATTCCAGGGGGCCAAGAAACTGGGAACAGATAAAAGTGCCTGGGCTCCATCCTTCAATGTCGATGAGTGATTTTATGAGTCACATTGGGAACTGTATTTCAGAACAAATGACTTCCGGAAATCCACCTTCTGCCGATCAACAGTCGGAATACCAGGATATTCTTGAGGACATTGCGCAGTACCTGTTAAGTGACACTCAATTAACTGCAGCAGATGAGAGTCATGAGACATCTCTCATGTCCAGGGTCAATTCTCTTTGTTGCCTTTTGCAGAAGGACCCCACTTCTCTTCAGACTTCGCAGGTTGAAGTTGAAGGCATAGATGGTGGAAGGGATATTCAGCTTAACCACACTCCTGGTGGTATATCTGGAGCTGATATGCAGGCTCCTGAAGAGGATATGAAGGATGTTCCTGGAGGAAAGAAGGCACCGGGAATGTCGAGAAAAGACTCCTTCGGGGAATTGCTGTTTCACCTCCCTCGCATCGCCTCTCTTCCCTCACTTCCAAAAGTCTTGTTCAACATTTCAGAAGATTGTGAGAGTCAAGGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

539

Amino Acids

58.96

Weight (kDa)

5.37

Isoelectric Point (pI)

55.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_TRF2_HOY1 PF24818 130 - 248 8.8e-62 TRF2/HOY1 PH-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24100 AT4G30780
fragaria_vesca FvH4_4g10180
malus_domestica MD00G1164000.v1.1 MD16G1259500.v1.1
prunus_persica Prupe.1G102300_v2.0.a1
pyrus_communis pycom13g28620 pycom16g22740
rosa_chinensis RchiOBHm_Chr4g0405561
rosa_laevigata RLG00000008836
rosa_multiflora Rmu_sc0005023.1_g000013
rosa_roxburghii Rroxscaffold_5G00350070
rosa_rugosa Rorug04G0060600 Rorug04G0060600
rosa_samantha Rh4AG132500 Rh4BG127400 Rh4CG140200 Rh4DG126800
rosa_wichuraiana Rw4G010730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1242
Acc36I ACCTGC 1 cut(s) 1357
AccB1I GGYRCC 1 cut(s) 1492
AccI GTMKAC 1 cut(s) 796
AccIII TCCGGA 1 cut(s) 1181
AciI CCGC 4 cut(s) 31, 293, 356, 838
AclWI GGATC 3 cut(s) 419, 903, 916
AcsI RAATTY 1 cut(s) 388
AcuI CTGAAG 4 cut(s) 607, 1077, 1340, 1476
AcyI GRCGYC 1 cut(s) 41
AfaI GTAC 5 cut(s) 303, 428, 440, 586, 1247
AfeI AGCGCT 1 cut(s) 119
AfiI CCNNNNNNNGG 4 cut(s) 293, 575, 899, 1040
AflII CTTAAG 1 cut(s) 790
AflIII ACRYGT 1 cut(s) 776
AjiI CACGTC 1 cut(s) 779
AjnI CCWGG 8 cut(s) 580, 927, 1070, 1105, 1218, 1307, 1420, 1477
AjuI GAANNNNNNNTTGG 2 cut(s) 584, 616
Alw26I GTCTC 2 cut(s) 623, 1286
AlwI GGATC 3 cut(s) 419, 903, 916
AlwNI CAGNNNCTG 1 cut(s) 1249
Aor13HI TCCGGA 1 cut(s) 1181
Aor51HI AGCGCT 1 cut(s) 119
AoxI GGCC 5 cut(s) 103, 508, 549, 874, 1076
ApeKI GCWGC 4 cut(s) 58, 290, 353, 1274
ApoI RAATTY 1 cut(s) 388
AseI ATTAAT 1 cut(s) 254
Asp700I GAANNNNTTC 1 cut(s) 255
AspLEI GCGC 2 cut(s) 120, 1243
AspS9I GGNCC 7 cut(s) 104, 281, 550, 578, 1014, 1076, 1342
AsuC2I CCSGG 1 cut(s) 1497
AsuHPI GGTGA 6 cut(s) 76, 167, 458, 813, 933, 1529
AvaII GGWCC 4 cut(s) 281, 578, 1014, 1342
BaeI ACNNNNGTAYC 2 cut(s) 430, 463
BamHI GGATCC 1 cut(s) 908
BanI GGYRCC 1 cut(s) 1492
BanII GRGCYC 2 cut(s) 239, 1113
BbsI GAAGAC 2 cut(s) 87, 861
BbvCI CCTCAGC 1 cut(s) 639
BbvI GCAGC 4 cut(s) 45, 302, 340, 1286
BccI CCATC 5 cut(s) 569, 680, 952, 1122, 1382
BceAI ACGGC 1 cut(s) 28
BciT130I CCWGG 8 cut(s) 582, 929, 1072, 1107, 1220, 1309, 1422, 1479
BcnI CCSGG 1 cut(s) 1497
BcoDI GTCTC 2 cut(s) 623, 1286
BfaI CTAG 4 cut(s) 207, 606, 645, 1035
BfmI CTRYAG 2 cut(s) 288, 1272
BfoI RGCGCY 1 cut(s) 121
BfrI CTTAAG 1 cut(s) 790
BfuAI ACCTGC 1 cut(s) 1357
BisI GCNGC 4 cut(s) 59, 291, 354, 1275
BlsI GCNGC 4 cut(s) 60, 292, 355, 1276
Bme1390I CCNGG 9 cut(s) 582, 929, 1072, 1107, 1220, 1309, 1422, 1479, 1497
Bme18I GGWCC 4 cut(s) 281, 578, 1014, 1342
BmgBI CACGTC 1 cut(s) 779
BmgT120I GGNCC 7 cut(s) 104, 281, 550, 578, 1014, 1076, 1342
BmiI GGNNCC 8 cut(s) 106, 282, 910, 1077, 1112, 1344, 1452, 1494
BmrFI CCNGG 9 cut(s) 582, 929, 1072, 1107, 1220, 1309, 1422, 1479, 1497
BmrI ACTGGG 1 cut(s) 1096
BmsI GCATC 4 cut(s) 392, 947, 1018, 1557
BmuI ACTGGG 1 cut(s) 1096
BoxI GACNNNNGTC 1 cut(s) 777
BpiI GAAGAC 2 cut(s) 87, 861
BpmI CTGGAG 3 cut(s) 345, 1455, 1500
Bpu10I CCTNAGC 4 cut(s) 227, 273, 639, 1039
BpuEI CTTGAG 2 cut(s) 497, 1250
BpuMI CCSGG 1 cut(s) 1497
BsaHI GRCGYC 1 cut(s) 41
BsaJI CCNNGG 6 cut(s) 87, 859, 877, 1071, 1106, 1308
BsaWI WCCGGW 1 cut(s) 1181
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 4 cut(s) 293, 575, 899, 1040
Bse118I RCCGGY 2 cut(s) 61, 892
Bse1I ACTGG 2 cut(s) 532, 1091
Bse3DI GCAATG 2 cut(s) 728, 1236
BseAI TCCGGA 1 cut(s) 1181
BseBI CCWGG 8 cut(s) 582, 929, 1072, 1107, 1220, 1309, 1422, 1479
BseDI CCNNGG 6 cut(s) 87, 859, 877, 1071, 1106, 1308
BseGI GGATG 2 cut(s) 1114, 1477
BseLI CCNNNNNNNGG 4 cut(s) 293, 575, 899, 1040
BseMI GCAATG 2 cut(s) 728, 1236
BseMII CTCAG 5 cut(s) 241, 287, 653, 687, 1053
BseNI ACTGG 2 cut(s) 532, 1091
BseRI GAGGAG 2 cut(s) 107, 110
BseXI GCAGC 4 cut(s) 45, 302, 340, 1286
BseYI CCCAGC 2 cut(s) 175, 420
BsgI GTGCAG 1 cut(s) 26
BshFI GGCC 5 cut(s) 105, 510, 551, 876, 1078
BshNI GGYRCC 1 cut(s) 1492
BsiSI CCGG 5 cut(s) 21, 62, 893, 1182, 1496
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 4 cut(s) 293, 575, 899, 1040
BsmAI GTCTC 2 cut(s) 623, 1286
BsmFI GGGAC 1 cut(s) 158
BsnI GGCC 5 cut(s) 105, 510, 551, 876, 1078
Bsp1286I GDGCHC 2 cut(s) 239, 1113
Bsp13I TCCGGA 1 cut(s) 1181
Bsp143I GATC 6 cut(s) 25, 75, 411, 807, 908, 1201
BspACI CCGC 4 cut(s) 31, 293, 356, 838
BspANI GGCC 5 cut(s) 105, 510, 551, 876, 1078
BspCNI CTCAG 5 cut(s) 240, 286, 652, 686, 1052
BspEI TCCGGA 1 cut(s) 1181
BspHI TCATGA 2 cut(s) 142, 1288
BspLI GGNNCC 8 cut(s) 106, 282, 910, 1077, 1112, 1344, 1452, 1494
BspMAI CTGCAG 2 cut(s) 292, 1276
BspMI ACCTGC 1 cut(s) 1357
BspPI GGATC 3 cut(s) 419, 903, 916
BspQI GCTCTTC 1 cut(s) 227
BspT107I GGYRCC 1 cut(s) 1492
BspTI CTTAAG 1 cut(s) 790
BsrDI GCAATG 2 cut(s) 728, 1236
BsrFI RCCGGY 2 cut(s) 61, 892
BsrI ACTGG 2 cut(s) 532, 1091
BssAI RCCGGY 2 cut(s) 61, 892
BssECI CCNNGG 6 cut(s) 87, 859, 877, 1071, 1106, 1308
BssMI GATC 6 cut(s) 25, 75, 411, 807, 908, 1201
BssNI GRCGYC 1 cut(s) 41
BssT1I CCWWGG 3 cut(s) 87, 859, 877
Bst2UI CCWGG 8 cut(s) 582, 929, 1072, 1107, 1220, 1309, 1422, 1479
Bst4CI ACNGT 4 cut(s) 566, 738, 1160, 1209
Bst6I CTCTTC 4 cut(s) 227, 1359, 1452, 1563
BstACI GRCGYC 1 cut(s) 41
BstAFI CTTAAG 1 cut(s) 790
BstAPI GCANNNNNTGC 1 cut(s) 353
BstC8I GCNNGC 6 cut(s) 270, 666, 693, 697, 701, 1449
BstDEI CTNAG 5 cut(s) 227, 273, 639, 673, 1039
BstENI CCTNNNNNAGG 1 cut(s) 1038
BstF5I GGATG 2 cut(s) 1114, 1477
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 2 cut(s) 120, 1243
BstKTI GATC 6 cut(s) 28, 78, 414, 810, 911, 1204
BstMAI GTCTC 2 cut(s) 623, 1286
BstMBI GATC 6 cut(s) 25, 75, 411, 807, 908, 1201
BstMWI GCNNNNNNNGC 7 cut(s) 30, 176, 353, 362, 516, 557, 649
BstNI CCWGG 8 cut(s) 582, 929, 1072, 1107, 1220, 1309, 1422, 1479
BstPAI GACNNNNGTC 1 cut(s) 777
BstSCI CCNGG 9 cut(s) 580, 927, 1070, 1105, 1218, 1307, 1420, 1477, 1495
BstSFI CTRYAG 2 cut(s) 288, 1272
BstV1I GCAGC 4 cut(s) 45, 302, 340, 1286
BstV2I GAAGAC 2 cut(s) 87, 861
BstX2I RGATCY 1 cut(s) 908
BstXI CCANNNNNNTGG 2 cut(s) 1086, 1421
BstYI RGATCY 1 cut(s) 908
BsuRI GGCC 5 cut(s) 105, 510, 551, 876, 1078
BtgZI GCGATG 1 cut(s) 1534
BtrI CACGTC 1 cut(s) 779
BtsCI GGATG 2 cut(s) 1114, 1477
BtsIMutI CAGTG 2 cut(s) 539, 773
BveI ACCTGC 1 cut(s) 1357
Cac8I GCNNGC 6 cut(s) 270, 666, 693, 697, 701, 1449
CaiI CAGNNNCTG 1 cut(s) 1249
CciI TCATGA 2 cut(s) 142, 1288
CfoI GCGC 2 cut(s) 120, 1243
Cfr10I RCCGGY 2 cut(s) 61, 892
Cfr13I GGNCC 7 cut(s) 104, 281, 550, 578, 1014, 1076, 1342
CseI GACGC 1 cut(s) 49
CsiI ACCWGGT 1 cut(s) 580
Csp6I GTAC 5 cut(s) 302, 427, 439, 585, 1246
CviAII CATG 5 cut(s) 101, 143, 547, 1289, 1303
CviQI GTAC 5 cut(s) 302, 427, 439, 585, 1246
DdeI CTNAG 5 cut(s) 227, 273, 639, 673, 1039
DpnI GATC 6 cut(s) 27, 77, 413, 809, 910, 1203
DpnII GATC 6 cut(s) 25, 75, 411, 807, 908, 1201
Eam1104I CTCTTC 4 cut(s) 227, 1359, 1452, 1563
EarI CTCTTC 4 cut(s) 227, 1359, 1452, 1563
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 87, 859, 877
Eco147I AGGCCT 2 cut(s) 510, 876
Eco24I GRGCYC 2 cut(s) 239, 1113
Eco32I GATATC 1 cut(s) 544
Eco47I GGWCC 4 cut(s) 281, 578, 1014, 1342
Eco47III AGCGCT 1 cut(s) 119
Eco57I CTGAAG 4 cut(s) 607, 1077, 1340, 1476
EcoNI CCTNNNNNAGG 1 cut(s) 1038
EcoO109I RGGNCCY 2 cut(s) 281, 1342
EcoRII CCWGG 8 cut(s) 580, 927, 1070, 1105, 1218, 1307, 1420, 1477
EcoRV GATATC 1 cut(s) 544
EcoT14I CCWWGG 3 cut(s) 87, 859, 877
EcoT38I GRGCYC 2 cut(s) 239, 1113
ErhI CCWWGG 3 cut(s) 87, 859, 877
FaeI CATG 5 cut(s) 104, 146, 550, 1292, 1306
FaqI GGGAC 1 cut(s) 158
FatI CATG 5 cut(s) 100, 142, 546, 1288, 1302
FauI CCCGC 1 cut(s) 286
FblI GTMKAC 1 cut(s) 796
Fnu4HI GCNGC 4 cut(s) 59, 291, 354, 1275
FokI GGATG 2 cut(s) 1101, 1484
FriOI GRGCYC 2 cut(s) 239, 1113
Fsp4HI GCNGC 4 cut(s) 59, 291, 354, 1275
FspBI CTAG 4 cut(s) 207, 606, 645, 1035
FspI TGCGCA 1 cut(s) 1242
GlaI GCGC 2 cut(s) 119, 1242
GluI GCNGC 4 cut(s) 59, 291, 354, 1275
GsaI CCCAGC 2 cut(s) 179, 424
GsuI CTGGAG 3 cut(s) 345, 1455, 1500
HaeII RGCGCY 1 cut(s) 121
HaeIII GGCC 5 cut(s) 105, 510, 551, 876, 1078
HapII CCGG 5 cut(s) 21, 62, 893, 1182, 1496
HgaI GACGC 1 cut(s) 49
HhaI GCGC 2 cut(s) 120, 1243
Hin1I GRCGYC 1 cut(s) 41
Hin1II CATG 5 cut(s) 104, 146, 550, 1292, 1306
Hin6I GCGC 2 cut(s) 118, 1241
HinP1I GCGC 2 cut(s) 118, 1241
HincII GTYRAC 1 cut(s) 797
HindII GTYRAC 1 cut(s) 797
HindIII AAGCTT 3 cut(s) 266, 374, 758
HinfI GANTC 9 cut(s) 83, 818, 866, 986, 1067, 1144, 1285, 1514, 1606
HpaII CCGG 5 cut(s) 21, 62, 893, 1182, 1496
HphI GGTGA 6 cut(s) 76, 167, 458, 813, 933, 1529
Hpy166II GTNNAC 2 cut(s) 728, 797
Hpy188I TCNGA 7 cut(s) 541, 676, 1057, 1168, 1213, 1359, 1594
Hpy8I GTNNAC 2 cut(s) 728, 797
Hpy99I CGWCG 1 cut(s) 43
HpyCH4III ACNGT 4 cut(s) 566, 738, 1160, 1209
HpyCH4IV ACGT 1 cut(s) 778
HpyCH4V TGCA 9 cut(s) 7, 290, 473, 703, 721, 950, 1274, 1336, 1447
HpyF10VI GCNNNNNNNGC 7 cut(s) 30, 176, 353, 362, 516, 557, 649
HpyF3I CTNAG 5 cut(s) 227, 273, 639, 673, 1039
HpySE526I ACGT 1 cut(s) 778
Hsp92I GRCGYC 1 cut(s) 41
Hsp92II CATG 5 cut(s) 104, 146, 550, 1292, 1306
HspAI GCGC 2 cut(s) 118, 1241
Kpn2I TCCGGA 1 cut(s) 1181
Kzo9I GATC 6 cut(s) 25, 75, 411, 807, 908, 1201
LguI GCTCTTC 1 cut(s) 227
LmnI GCTCC 7 cut(s) 97, 125, 184, 1030, 1116, 1436, 1456
Lsp1109I GCAGC 4 cut(s) 45, 302, 340, 1286
LweI GCATC 4 cut(s) 392, 947, 1018, 1557
MabI ACCWGGT 1 cut(s) 580
MaeI CTAG 4 cut(s) 207, 606, 645, 1035
MaeII ACGT 1 cut(s) 778
MaeIII GTNAC 3 cut(s) 770, 1145, 1256
MalI GATC 6 cut(s) 27, 77, 413, 809, 910, 1203
MboI GATC 6 cut(s) 25, 75, 411, 807, 908, 1201
MboII GAAGA 8 cut(s) 92, 244, 613, 866, 1346, 1469, 1550, 1607
MflI RGATCY 1 cut(s) 908
MhlI GDGCHC 2 cut(s) 239, 1113
MluCI AATT 7 cut(s) 68, 251, 255, 388, 1265, 1315, 1526
MlyI GAGTC 5 cut(s) 77, 1153, 1294, 1508, 1615
MmeI TCCRAC 1 cut(s) 1191
MroI TCCGGA 1 cut(s) 1181
MroXI GAANNNNTTC 1 cut(s) 255
MspA1I CMGCKG 2 cut(s) 293, 992
MspCI CTTAAG 1 cut(s) 790
MspI CCGG 5 cut(s) 21, 62, 893, 1182, 1496
MspR9I CCNGG 9 cut(s) 582, 929, 1072, 1107, 1220, 1309, 1422, 1479, 1497
MvaI CCWGG 8 cut(s) 582, 929, 1072, 1107, 1220, 1309, 1422, 1479
MwoI GCNNNNNNNGC 7 cut(s) 30, 176, 353, 362, 516, 557, 649
NciI CCSGG 1 cut(s) 1497
NdeII GATC 6 cut(s) 25, 75, 411, 807, 908, 1201
NlaIII CATG 5 cut(s) 104, 146, 550, 1292, 1306
NlaIV GGNNCC 8 cut(s) 106, 282, 910, 1077, 1112, 1344, 1452, 1494
NmuCI GTSAC 3 cut(s) 770, 1145, 1256
NsbI TGCGCA 1 cut(s) 1242
PagI TCATGA 2 cut(s) 142, 1288
PceI AGGCCT 2 cut(s) 510, 876
PciSI GCTCTTC 1 cut(s) 227
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 4 cut(s) 818, 866, 986, 1067
PfoI TCCNGGA 2 cut(s) 927, 1477
PkrI GCNGC 4 cut(s) 60, 292, 355, 1276
PleI GAGTC 5 cut(s) 77, 1152, 1293, 1508, 1614
PpsI GAGTC 5 cut(s) 77, 1152, 1293, 1508, 1614
PpuMI RGGWCCY 2 cut(s) 281, 1342
PshAI GACNNNNGTC 1 cut(s) 777
PshBI ATTAAT 1 cut(s) 254
Psp5II RGGWCCY 2 cut(s) 281, 1342
Psp6I CCWGG 8 cut(s) 580, 927, 1070, 1105, 1218, 1307, 1420, 1477
PspFI CCCAGC 2 cut(s) 175, 420
PspGI CCWGG 8 cut(s) 580, 927, 1070, 1105, 1218, 1307, 1420, 1477
PspN4I GGNNCC 8 cut(s) 106, 282, 910, 1077, 1112, 1344, 1452, 1494
PspPI GGNCC 7 cut(s) 104, 281, 550, 578, 1014, 1076, 1342
PspPPI RGGWCCY 2 cut(s) 281, 1342
PsrI GAACNNNNNNTAC 2 cut(s) 480, 512
PstI CTGCAG 2 cut(s) 292, 1276
PstNI CAGNNNCTG 1 cut(s) 1249
PsuI RGATCY 1 cut(s) 908
PvuII CAGCTG 1 cut(s) 992
RsaI GTAC 5 cut(s) 303, 428, 440, 586, 1247
RsaNI GTAC 5 cut(s) 302, 427, 439, 585, 1246
SalI GTCGAC 1 cut(s) 795
SapI GCTCTTC 1 cut(s) 227
SatI GCNGC 4 cut(s) 59, 291, 354, 1275
Sau3AI GATC 6 cut(s) 25, 75, 411, 807, 908, 1201
Sau96I GGNCC 7 cut(s) 104, 281, 550, 578, 1014, 1076, 1342
SchI GAGTC 5 cut(s) 77, 1153, 1294, 1508, 1615
ScrFI CCNGG 9 cut(s) 582, 929, 1072, 1107, 1220, 1309, 1422, 1479, 1497
SduI GDGCHC 2 cut(s) 239, 1113
SexAI ACCWGGT 1 cut(s) 580
SfaNI GCATC 4 cut(s) 392, 947, 1018, 1557
SfcI CTRYAG 2 cut(s) 288, 1272
SinI GGWCC 4 cut(s) 281, 578, 1014, 1342
SmlI CTYRAG 3 cut(s) 512, 790, 1229
SmoI CTYRAG 3 cut(s) 512, 790, 1229
Sse9I AATT 7 cut(s) 68, 251, 255, 388, 1265, 1315, 1526
SseBI AGGCCT 2 cut(s) 510, 876
SsiI CCGC 4 cut(s) 31, 293, 356, 838
SspMI CTAG 4 cut(s) 207, 606, 645, 1035
StuI AGGCCT 2 cut(s) 510, 876
StyD4I CCNGG 9 cut(s) 580, 927, 1070, 1105, 1218, 1307, 1420, 1477, 1495
StyI CCWWGG 3 cut(s) 87, 859, 877
TaaI ACNGT 4 cut(s) 566, 738, 1160, 1209
TaiI ACGT 1 cut(s) 781
TaqI TCGA 4 cut(s) 78, 796, 1127, 1505
TaqII GACCGA 1 cut(s) 1002
TasI AATT 7 cut(s) 68, 251, 255, 388, 1265, 1315, 1526
TatI WGTACW 1 cut(s) 426
TfiI GAWTC 4 cut(s) 818, 866, 986, 1067
TscAI CASTG 2 cut(s) 539, 773
TseFI GTSAC 3 cut(s) 770, 1145, 1256
TseI GCWGC 4 cut(s) 58, 290, 353, 1274
Tsp45I GTSAC 3 cut(s) 770, 1145, 1256
TspDTI ATGAA 4 cut(s) 29, 131, 278, 1481
TspRI CASTG 2 cut(s) 539, 773
Vha464I CTTAAG 1 cut(s) 790
VpaK11BI GGWCC 4 cut(s) 281, 578, 1014, 1342
VspI ATTAAT 1 cut(s) 254
XagI CCTNNNNNAGG 1 cut(s) 1038
XapI RAATTY 1 cut(s) 388
XcmI CCANNNNNNNNNTGG 2 cut(s) 896, 1421
XmiI GTMKAC 1 cut(s) 796
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 4 cut(s) 207, 606, 645, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.